GCR115
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005705 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of GCR115 vs. TrEMBL
Match: A0A193KUN2 (GCR115 OS=Schmidtea mediterranea OX=79327 GN=gcr115 PE=2 SV=1) HSP 1 Score: 356.295 bits (913), Expect = 2.471e-113 Identity = 199/490 (40.61%), Postives = 284/490 (57.96%), Query Frame = 3 Query: 153 QPICWPMTSVSYLSMISSCSDINTDPILRRKCEDPNLFLPYIVDYIP-LHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLC--------YANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVXXXXXXXXXXXXYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKFCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMR--TPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPI-NHERHCKSHQRFFIGLKLCFISGITWILNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQS 1586 ICWP T +LSMIS C + L KCE+PN+FL IVDY P + + +VLYRN YC +C+ I +Y+++ N +C + K K + +Y A ML+ C Y+ F C +P C N ++F GY N ++WF F CC N S IC+ S R+ L Y++N ++Y + E V N++ + E+S L Y + LSL ++ LLI Y I +LQTSTSYMI+ IG NIA +E+ +L F C C+ C++ SI+FHISE ICIS Y+ SFW+DL+QMR + N RRN KR V F+ S +IP+ L+F + DF + ++C LK+ PL FYI+P+ ++L++TT++ +KIC YL SS+ I ++E ++ Q F +GLKLCFISGITWI N +M+ PN I+ D G+++SHFNGFVIVVCFLS + VRK I +++ Sbjct: 48 DEICWPFTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQF------SCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTS---NHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKA 528
BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000051772.1 (SMESG000051772.1) HSP 1 Score: 1045.03 bits (2701), Expect = 0.000e+0 Identity = 526/531 (99.06%), Postives = 527/531 (99.25%), Query Frame = 3 Query: 3 PISSHNYQQLPAYTSPFSIIYKFMKTMSPRNLISFLHILILVEVSNLQSQQPICWPMTSVSYLSMISSCSDINTDPILRRKCEDPNLFLPYIVDYIPLHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLCYANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVXXXXXXXXXXXXYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKFCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMRTPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPINHERHCKSHQRFFIGLKLCFISGITWILNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQSFME 1595 PISSHNYQQLPAYTS FSIIYKFMKTMSPRNLISFLHILILVEVSNLQSQQPICWPMTSVSYL MIS+CSDINTDPILRRKCEDPNLFLPYIVDYIPLHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLCYANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVNNNTNSSLNISIYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCK CELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMRTPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPINHERHCKSHQRFFIGLKLCFISGITWI NFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQSFME Sbjct: 25 PISSHNYQQLPAYTSTFSIIYKFMKTMSPRNLISFLHILILVEVSNLQSQQPICWPMTSVSYLFMISNCSDINTDPILRRKCEDPNLFLPYIVDYIPLHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLCYANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVNNNTNSSLNISIYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKLCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMRTPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPINHERHCKSHQRFFIGLKLCFISGITWIFNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQSFME 555
BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000051770.1 (SMESG000051770.1) HSP 1 Score: 356.295 bits (913), Expect = 6.871e-117 Identity = 199/490 (40.61%), Postives = 284/490 (57.96%), Query Frame = 3 Query: 153 QPICWPMTSVSYLSMISSCSDINTDPILRRKCEDPNLFLPYIVDYIP-LHRRGSKSVLYRNRYCIRCNKIKEYEYVNTNNTFRCSKKIVLGKGKQKENYLALMLFKRLC--------YANFHFPESLRCSLPEEKCKIPETVNEDTFIGYGIDNSILRWFRFQGSECCLNYSEICMPISRRRTPLLIYALNEMIYPDDFETVXXXXXXXXXXXXYRQEESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKFCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMR--TPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQVCSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYLRSSIPI-NHERHCKSHQRFFIGLKLCFISGITWILNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQS 1586 ICWP T +LSMIS C + L KCE+PN+FL IVDY P + + +VLYRN YC +C+ I +Y+++ N +C + K K + +Y A ML+ C Y+ F C +P C N ++F GY N ++WF F CC N S IC+ S R+ L Y++N ++Y + E V N++ + E+S L Y + LSL ++ LLI Y I +LQTSTSYMI+ IG NIA +E+ +L F C C+ C++ SI+FHISE ICIS Y+ SFW+DL+QMR + N RRN KR V F+ S +IP+ L+F + DF + ++C LK+ PL FYI+P+ ++L++TT++ +KIC YL SS+ I ++E ++ Q F +GLKLCFISGITWI N +M+ PN I+ D G+++SHFNGFVIVVCFLS + VRK I +++ Sbjct: 48 DEICWPFTKNFFLSMISKCPSSYKNKYLSEKCENPNIFLSDIVDYRPVIAQSQPMNVLYRNMYCAKCHNIVKYDWIKVNRKLKCKINLKSRKIKDENSYYARMLYNHQCEFVIDQRQYSQF------SCMIPIGVCDGYLLNNVESFYGYIEKNPTIKWFEFPSLNCCKNNSRICIVNSVGRSKLFYYSINSLLYNNQIEVVKNSSLKNTTS---NHEDSFLVYALDIAATSLSLVASILLISAYLIIPELQTSTSYMIVAIGFNIACLELTNLLSTFKCDSCQFLACKFHGIFSIFFHISETICISNYITSFWNDLNQMRRHSQNGIHEKRRNLKRTVRFYLSSAVIPLIFSSSYLLFDRFDFINFKEKNKTRMCFSLKMIPLICFYIIPVFVVLIYTTIVNMKICFYLHSSVKISSNETQNQTRQHFLLGLKLCFISGITWIFNLIMSVFPNRIVLDCGMIISHFNGFVIVVCFLSGRMVRKCIFKKA 528
BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000033614.1 (SMESG000033614.1) HSP 1 Score: 56.6102 bits (135), Expect = 3.709e-8 Identity = 57/273 (20.88%), Postives = 124/273 (45.42%), Query Frame = 3 Query: 780 ESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKFCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMRTPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLIFQKNDFFKTGRSRRLQV----CSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYL-RSSIPINHERHCKSHQRFFIGLKLCFISGITWILNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQ 1583 E+ ++++S F LSL S F LII+Y T + + Y+ I + I + + +L+ + + + C+Y + + +S + ++ Y L T + + R +K +F C + + ++I + D KT + R Q C + +Y +P+ L+LL+ + C + L +++ + + ++ ++L + G+ ++L + FT I+ LG +V+ GF++ +CF+ +K VR ++ + Sbjct: 279 ETVIYHISFAFTC-LSLVSLFFLIILYLTHQVFKNLGGYLTICLSICLWLINFMSLLVTNT-QIVKSASCKYLAIFHHFVALSTFVWMAVYALDL---LQTFGTKSASINKNRGWKTFCIYFICVFAVSGCVVGISVIIEYFD--KTNSTLRPQYGGPHCWFTSKFGGLIWYFIPVCLLLLWNIITCGFVFYQLFKTAKECRIINNNDLNSTLWLCMRLTAVFGVGYVLQIIAIFTQLNILISLGSMVNCLQGFMLTLCFVCTKRVRSQLRNR 544
BLAST of GCR115 vs. Planmine SMEST
Match: SMESG000033615.1 (SMESG000033615.1) HSP 1 Score: 53.1434 bits (126), Expect = 2.917e-7 Identity = 63/276 (22.83%), Postives = 130/276 (47.10%), Query Frame = 3 Query: 780 ESTLFYVSHLFVVFLSLGSAFLLIIVYATIRDLQTSTSYMIIVIGINIAFMEIQEILMIFLCKFCELSICEYFSTLSIYFHISEAICISGYVYSFWSDLHQMRTPNKQSSMRRNYKRNVGFFSCSILIPMALCCGNLI---FQKNDFFKTGRSRRLQV----CSVLKLYPLFGFYIVPLLLILLFTTVICIKICLYL-RSSIPINHERHCKSHQRFFIGLKLCFISGITWILNFLMAFTPNTIIRDLGVVVSHFNGFVIVVCFLSSKTVRKYIKEQ 1583 E+ ++++S F LSL S F LII+Y T + + Y+ I + I + + L++ + E + C+Y L+I+ H + +S +V+ L ++T + + N KR + FS LI + G+++ F KT + R Q C + +Y +P+ L+LL+ ++C + L +++ + ++ ++ ++L + G+ ++L FT I+ LG +V+ GF++ +CF+ +K R ++ + Sbjct: 122 ETVIYHISFAFTC-LSLVSLFFLIILYLTHQVFKNFGGYLTICLSICLWLINFMS-LLVTNTQIVESASCKY---LAIFHHF---VALSTFVWMAVYALDLLQTFGTKL-VNANKKRGLKSFSVYCLIGFS-TSGSVVGISVITEYFDKTNSTLRPQYGGPYCWFTSKFGGLIYYFIPVCLLLLWNFIMCSFVFYQLFKTAKECRIVKKKDANSTLWLCMRLTAVFGVGYVLQIFAIFTQLEILISLGSMVNCLQGFMLTLCFVCTKRARSLLRNR 387 The following BLAST results are available for this feature:
BLAST of GCR115 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of GCR115 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of GCR115 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of GCR115 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of GCR115 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR115 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of GCR115 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of GCR115 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005705 ID=SMED30005705|Name=GCR115|organism=Schmidtea mediterranea sexual|type=transcript|length=1628bpback to top protein sequence of SMED30005705-orf-1 >SMED30005705-orf-1 ID=SMED30005705-orf-1|Name=SMED30005705-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=532bp PISSHNYQQLPAYTSPFSIIYKFMKTMSPRNLISFLHILILVEVSNLQSQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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