SMED30005665
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005665 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of SMED30005665 vs. Planmine SMEST
Match: SMESG000074689.1 (SMESG000074689.1) HSP 1 Score: 765.377 bits (1975), Expect = 0.000e+0 Identity = 392/395 (99.24%), Postives = 393/395 (99.49%), Query Frame = 1 Query: 127 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILASSPGITRPPPKQFSTNTASIIETVFKERDXXXXXXXXXXXXXXXVNIWCSVQWCIKTSKKKNLKRNRTPNELQRSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFENNNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 1311 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSIL SSPGITRPPPKQFSTNTASIIETVFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQ+SYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFE NNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE Sbjct: 1 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILVSSPGITRPPPKQFSTNTASIIETVFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQKSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFEINNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 395
BLAST of SMED30005665 vs. Planmine SMEST
Match: SMESG000074689.1 (SMESG000074689.1) HSP 1 Score: 738.799 bits (1906), Expect = 0.000e+0 Identity = 381/395 (96.46%), Postives = 383/395 (96.96%), Query Frame = 1 Query: 127 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILASSPGITRPPPKQFSTNTASIIETVFKERDXXXXXXXXXXXXXXXVNIWCSVQWCIKTSKKKNLKRNRTPNELQRSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFENNNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 1311 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSIL SSPGITRPPPKQF +FKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQ+SYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFE NNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE Sbjct: 1 MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILVSSPGITRPPPKQF----------IFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQKSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFEINNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 385
BLAST of SMED30005665 vs. Planmine SMEST
Match: SMESG000074689.1 (SMESG000074689.1) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 361/364 (99.18%), Postives = 362/364 (99.45%), Query Frame = 1 Query: 220 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILASSPGITRPPPKQFSTNTASIIETVFKERDXXXXXXXXXXXXXXXVNIWCSVQWCIKTSKKKNLKRNRTPNELQRSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFENNNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 1311 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSIL SSPGITRPPPKQFSTNTASIIETVFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQ+SYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFE NNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE Sbjct: 1 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILVSSPGITRPPPKQFSTNTASIIETVFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQKSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFEINNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 364
BLAST of SMED30005665 vs. Planmine SMEST
Match: SMESG000074689.1 (SMESG000074689.1) HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0 Identity = 350/364 (96.15%), Postives = 352/364 (96.70%), Query Frame = 1 Query: 220 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILASSPGITRPPPKQFSTNTASIIETVFKERDXXXXXXXXXXXXXXXVNIWCSVQWCIKTSKKKNLKRNRTPNELQRSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFENNNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 1311 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSIL SSPGITRPPPKQF +FKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQ+SYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFE NNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE Sbjct: 1 MIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVSKDNSVPSILVSSPGITRPPPKQF----------IFKERDIILISLIAALLLSILVNIWCSVQWCIKTSKKKNLKRNRTPNELQKSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFEINNDICSGSRNSLSTPIYGHVVYAPVQYVDPSMLIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPRKPDEILSDKE 354
BLAST of SMED30005665 vs. Planmine SMEST
Match: SMESG000074692.1 (SMESG000074692.1) HSP 1 Score: 176.792 bits (447), Expect = 1.121e-50 Identity = 127/389 (32.65%), Postives = 203/389 (52.19%), Query Frame = 1 Query: 166 IFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQITENSIDKSFGVFLKCPFNNQIDFCIKDCFSLDSCTNAKCNSKAVEKQNFTCDFSSTVNSITFSYRFISLSESSLKYSKQVSFGCFFRGKTSSKIVNVS-------KDNSVPSILASSPGITRPPPKQFSTNTASIIETV-------FKERDXXXXXXXXXXXXXXXVNIWCSVQWCIKTSKKKNLKRNRTPNELQRSYPMTDLNSNHYSNSMSSQYDPPENIKRTRSQPTLPHDDRITLIKSNSNTSYNTGSISSRPKYSRPNTMTFENNNDICSGSRNSLSTPIYGH-VVYAPVQYVDPSM--LIYGPQYSTSYFPHSAAVENKSLNYLDIVNEQDCPERQNSSVFQPLPR 1281 + L I+ + C ++ I I P +++G +L V C +T+ S+DK + L CP N + +CIK+C SLD+CT KC+ K+++ Q F C+ TV S+TFSY+FI L S + KQ+ F C FRGK+ ++IV +S ++ I ++ I K+F+ N + + FK R +++ L+ L +S+ +NIWC Q C++ K + L++ + +S +N ++SQY E +KR +S P L ++D NS+ T S +P Y R + TFE + + +GS +SL PIY H +V AP+Q +D SM L+Y +S S+ + NKS + LD EQ+ Q S FQP + Sbjct: 7 LILTIYSFGYILCLDVNIYIEPKTVRVGEELQVTCAVTDESLDKGISMLLTCPNNGEYGYCIKNCNSLDACTADKCDRKSIKDQPFQCNLKETVKSLTFSYKFI-LRNSFINNRKQLPFKCSFRGKSITEIVEISTIPLNENTESRFAQIPMTTDKILTEYNKKFNDNEQDGNKNINQSDGNLFKFRSLLIFGLLLGLFISLFLNIWCGAQCCLRKLKFRKLEKMDQEKQCYQS-----------NNELNSQYYCQEYLKRAKSHPNLTNND------FNSSRKLTTSSAYRKPLY-RGSIQTFE-DVEPHNGSMHSLMNPIYNHQMVLAPIQCIDQSMLPLLYPTSFSPSHNIPTQVDYNKSQSNLDTFAEQEIHRNQGESQFQPFQK 375 The following BLAST results are available for this feature:
BLAST of SMED30005665 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005665 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005665 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005665 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005665 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005665 ID=SMED30005665|Name=SMED30005665|organism=Schmidtea mediterranea sexual|type=transcript|length=1355bpback to top protein sequence of SMED30005665-orf-1 >SMED30005665-orf-1 ID=SMED30005665-orf-1|Name=SMED30005665-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=400bp MGIFTIHFENKWQIFLQIFFLHSVSCQNLEIMIAPNQIQIGNDLNVICQIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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