Malic enzyme

Overview


Name	Malic enzyme
Smed ID	SMED30005657
Length (bp)	2047

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Malic enzyme (SMED30005657) t-SNE clustered cells

Violin plots show distribution of expression levels for Malic enzyme (SMED30005657) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Malic enzyme (SMED30005657) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Malic enzyme (SMED30005657) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005657

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30005657	SMESG000045895.1	dd_Smed_v4_2140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30005657	SMESG000045895.1	dd_Smed_v4_2140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30005657	SMESG000045895.1	dd_Smed_v4_2140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30005657	SMESG000045895.1	dd_Smed_v4_2140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
reproductive organ	SMED30005657	SMESG000045895.1	Contig22502	newmark_ests	PMID:28434803 Rouhana et al., 2017
reproductive organ	SMED30005657	SMESG000045895.1	Contig22502	uc_Smed_v2	PMID:28434803 Rouhana et al., 2017
epidermis	SMED30005657	SMESG000045895.1	dd_Smed_v4_2140_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

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Homology

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BLAST of Malic enzyme vs. Ensembl Human
Match: ME1 (malic enzyme 1 [Source:HGNC Symbol;Acc:HGNC:6983])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 296/568 (52.11%), Postives = 395/568 (69.54%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            +  QRG  + R+P +NK  AF L+ERQ L IHGLLPP  N+ E Q LR + N   ++ D +R+L L+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+Y L+FR+PRGL+ITIHD+ +I  +L  WPE ++KA+VV+DGERILGLGDLG  GMGIPVGKL+LYTA  G+ P   LPV+LDVGT+N++LL DPLY+G+R +R+RG  Y DF+D FM+AV  KYG  CLIQFEDFANVNAFR+L+KYRN YCTFNDDIQGTASV + GLL A +I   KL D   LFQGAG AA GIA L+  AL + G  +E+AI KI++ D  GL+VK R           + + +   F  + +  K L   V+EIKPT LIG A + G F+  IL  +  F +RPI+FALSNPTSKAEC+A + Y  T G+ I+A GSPF PV   + +     Q NNSY+FPG+AL ++   +R +T+++F+  AE +A+QVSD+ L +GR++P L TI+DVSL+IA  I + A+    A  +PEP++K  ++    Y+  Y   LP+ Y +P E+ K +
Sbjct:   10 HTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGR----------ASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQ 567

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BLAST of Malic enzyme vs. Ensembl Human
Match: ME3 (malic enzyme 3 [Source:HGNC Symbol;Acc:HGNC:6985])

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 298/569 (52.37%), Postives = 400/569 (70.30%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M    +   DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK ++  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+ GK Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   KL +++F+FQGAG AA GIA LL  AL + G  + EA  KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D K FI  Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IA+ + +YA+   +A+++PEPKDK  ++    Y P Y     + Y +P E    + +
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV 604

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Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M    +   DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK ++  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+ GK Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   KL +++F+FQGAG AA GIA LL  AL + G  + EA  KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D K FI  Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IA+ + +YA+   +A+++PEPKDK  ++    Y P Y     + Y +P E    + +
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV 604

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BLAST of Malic enzyme vs. Ensembl Human
Match: ME3 (malic enzyme 3 [Source:HGNC Symbol;Acc:HGNC:6985])

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 292/540 (54.07%), Postives = 390/540 (72.22%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYI 1789
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M    +   DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK ++  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+ GK Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   KL +++F+FQGAG AA GIA LL  AL + G  + EA  KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D K FI  Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IA+ + +YA+   +A+++PEPKDK  ++
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFV 575

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BLAST of Malic enzyme vs. Ensembl Human
Match: ME2 (malic enzyme 2 [Source:HGNC Symbol;Acc:HGNC:6984])

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 276/561 (49.20%), Postives = 397/561 (70.77%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++K++G  ++ +PR NKG AF L+ERQ+LG+ GLLPP + T + QALR   NL+K+   L ++++++ +Q+RNE LFY+++ D++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +++ I++NWPE  VKA+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P   LPV +DVGTDN  LL DP Y+G+  KR R + Y D +D FM+A+ ++YGR  LIQFEDF N NAFR L KYR  YCTFNDDIQGTA+V + GLL A K+ ++ + ++  LF GAG AA GIA L+  +++E+G +E+EA  KI+MFD  GLLVK R       +   T S   +I            +AV  +KP+ +IG AG   +F  +++  + +  +RP++FALSNPT++AECTA EAYT T+G+C++A GSPF PV   D +VF   Q NN YIFPG+ALA+I+   R +++ +F+ AA+ L  Q++DE+LAQGR++P L  I++VS+ IA+ + EY ++  +A  +PEP+DK +Y+ +  +   Y   LP+ Y++P
Sbjct:   20 HIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESI-------PDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWP 573

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BLAST of Malic enzyme vs. Ensembl Celegans
Match: men-1 (Malic enzyme [Source:UniProtKB/TrEMBL;Acc:Q9U296])

HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0
Identity = 274/574 (47.74%), Postives = 388/574 (67.60%), Query Frame = 2

Query:  137 SFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMY--IKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDL-AQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            + +KL++   +  ++RGID+L+ P +NKG AF+L ERQ LG+HGLLPP   T E+QA R +  LR+  D+L +++ L SLQDRNE L+Y+V+ DNV+  +PI+YTPTVG ACQ +G ++R P+GLYITI+D     I +IL NWP + V+A+V++DGERILGLGDLG YG+GIPVGKL+LY ALAG+ P   LPV+LDVGTDN +LL+DP Y G+R KR+RG  Y   +D FM+A  +++GR  LIQFEDF N NA+R+L +Y+ +YC FNDDIQGTA+V + GLL + +I  +KL     +F GAG AATG+A +  R +++ G +EEEA  +IYM D++GL+   R     E         ++  F KD+   K L E VK +KP  LIGA+ V G F  +I+  +     RPI+FALSNPTSKAECTA  AY  T+G  ++A GSPF  V    K++   Q NN+YIFPG+AL  ++F+ + + + LF+ AA  +A+ VS++ L    RV+P L  I+++S++IAV + EY +   +A  HP+P D   +I ++ Y+  Y   +   Y++P
Sbjct:   35 ALHKLYRPERTTPQKRGIDLLKSPGLNKGMAFSLHERQYLGVHGLLPPAFMTEEQQAYRIITKLRQQPDNLAKYIQLDSLQDRNEKLYYRVLCDNVKELMPIVYTPTVGQACQHFGFIYRNPKGLYITINDNSISKIHQILANWPTENVRAIVITDGERILGLGDLGTYGIGIPVGKLALYVALAGIRPEWCLPVILDVGTDNSELLNDPFYTGLRRKRVRGPEYDTLVDNFMKAATKRFGRDTLIQFEDFGNQNAYRLLDRYKGEYCMFNDDIQGTAAVVVAGLLASTRITKKKLSQERIVFLGAGGAATGVAEMCVRQMMDEGLSEEEACGRIYMVDIEGLITTSRSKSLGE---------RHVKFAKDLPDTKNLLEVVKTVKPGALIGASTVAGAFTEDIIKEMARLNPRPIIFALSNPTSKAECTAETAYRCTNGAVLFASGSPFENVEMNGKLYKPGQGNNAYIFPGVALGAVLFRTKNIPDKLFLLAARMVAEAVSEKSLNTYSRVYPRLKDIRELSVKIAVEVGEYCYRHDLATLHPKPDDMEMFIRQNLYSVEYDELINKTYEWP 599

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Query:  137 SFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMY--IKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDL-AQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            + +KL++   +  ++RGID+L+ P +NKG AF+L ERQ LG+HGLLPP   T E+QA R +  LR+  D+L +++ L SLQDRNE L+Y+V+ DNV+  +PI+YTPTVG ACQ +G ++R P+GLYITI+D     I +IL NWP + V+A+V++DGERILGLGDLG YG+GIPVGKL+LY ALAG+ P   LPV+LDVGTDN +LL+DP Y G+R KR+RG  Y   +D FM+A  +++GR  LIQFEDF N NA+R+L +Y+ +YC FNDDIQGTA+V + GLL + +I  +KL     +F GAG AATG+A +  R +++ G +EEEA  +IYM D++GL+   R     E         ++  F KD+   K L E VK +KP  LIGA+ V G F  +I+  +     RPI+FALSNPTSKAECTA  AY  T+G  ++A GSPF  V    K++   Q NN+YIFPG+AL  ++F+ + + + LF+ AA  +A+ VS++ L    RV+P L  I+++S++IAV + EY +   +A  HP+P D   +I ++ Y+  Y   +   Y++P
Sbjct:   35 ALHKLYRPERTTPQKRGIDLLKSPGLNKGMAFSLHERQYLGVHGLLPPAFMTEEQQAYRIITKLRQQPDNLAKYIQLDSLQDRNEKLYYRVLCDNVKELMPIVYTPTVGQACQHFGFIYRNPKGLYITINDNSISKIHQILANWPTENVRAIVITDGERILGLGDLGTYGIGIPVGKLALYVALAGIRPEWCLPVILDVGTDNSELLNDPFYTGLRRKRVRGPEYDTLVDNFMKAATKRFGRDTLIQFEDFGNQNAYRLLDRYKGEYCMFNDDIQGTAAVVVAGLLASTRITKKKLSQERIVFLGAGGAATGVAEMCVRQMMDEGLSEEEACGRIYMVDIEGLITTSRSKSLGE---------RHVKFAKDLPDTKNLLEVVKTVKPGALIGASTVAGAFTEDIIKEMARLNPRPIIFALSNPTSKAECTAETAYRCTNGAVLFASGSPFENVEMNGKLYKPGQGNNAYIFPGVALGAVLFRTKNIPDKLFLLAARMVAEAVSEKSLNTYSRVYPRLKDIRELSVKIAVEVGEYCYRHDLATLHPKPDDMEMFIRQNLYSVEYDELINKTYEWP 599

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Query:  137 SFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMY--IKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDL-AQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            + +KL++   +  ++RGID+L+ P +NKG AF+L ERQ LG+HGLLPP   T E+QA R +  LR+  D+L +++ L SLQDRNE L+Y+V+ DNV+  +PI+YTPTVG ACQ +G ++R P+GLYITI+D     I +IL NWP + V+A+V++DGERILGLGDLG YG+GIPVGKL+LY ALAG+ P   LPV+LDVGTDN +LL+DP Y G+R KR+RG  Y   +D FM+A  +++GR  LIQFEDF N NA+R+L +Y+ +YC FNDDIQGTA+V + GLL + +I  +KL     +F GAG AATG+A +  R +++ G +EEEA  +IYM D++GL+   R     E         ++  F KD+   K L E VK +KP  LIGA+ V G F  +I+  +     RPI+FALSNPTSKAECTA  AY  T+G  ++A GSPF  V    K++   Q NN+YIFPG+AL  ++F+ + + + LF+ AA  +A+ VS++ L    RV+P L  I+++S++IAV + EY +   +A  HP+P D   +I ++ Y+  Y   +   Y++P
Sbjct:   35 ALHKLYRPERTTPQKRGIDLLKSPGLNKGMAFSLHERQYLGVHGLLPPAFMTEEQQAYRIITKLRQQPDNLAKYIQLDSLQDRNEKLYYRVLCDNVKELMPIVYTPTVGQACQHFGFIYRNPKGLYITINDNSISKIHQILANWPTENVRAIVITDGERILGLGDLGTYGIGIPVGKLALYVALAGIRPEWCLPVILDVGTDNSELLNDPFYTGLRRKRVRGPEYDTLVDNFMKAATKRFGRDTLIQFEDFGNQNAYRLLDRYKGEYCMFNDDIQGTAAVVVAGLLASTRITKKKLSQERIVFLGAGGAATGVAEMCVRQMMDEGLSEEEACGRIYMVDIEGLITTSRSKSLGE---------RHVKFAKDLPDTKNLLEVVKTVKPGALIGASTVAGAFTEDIIKEMARLNPRPIIFALSNPTSKAECTAETAYRCTNGAVLFASGSPFENVEMNGKLYKPGQGNNAYIFPGVALGAVLFRTKNIPDKLFLLAARMVAEAVSEKSLNTYSRVYPRLKDIRELSVKIAVEVGEYCYRHDLATLHPKPDDMEMFIRQNLYSVEYDELINKTYEWP 599

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BLAST of Malic enzyme vs. Ensembl Fly
Match: Men-b (gene:FBgn0029155 transcript:FBtr0301708)

HSP 1 Score: 598.201 bits (1541), Expect = 0.000e+0
Identity = 286/556 (51.44%), Postives = 388/556 (69.78%), Query Frame = 2

Query:  182 RGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            RGID +RDPR+NKG AF L+ERQ LGIHGL P    T EEQ   C I + +  + LN++L+L  L DRNE LF++ +++N+E  +PI+YTPTVGLACQ++GL++RRP GL+IT +D+ +I  +++NWPE  V+A+ V+DGERILGLGDLGA GMGIPVGKL+LYTALAG+ P   LP+++DVGT+N DLL+DPLY+G+R KR+ G+ Y +F+D FM+AVV++YG+  LIQFEDF N NAFR L KYRN YCTFNDDIQGTASV + GL  + +I  +  KD  FLF GAG AA GIA L  +A+++ G   EEA ++IYM D+DGLL K R +                 + KD+     LAE V  IKP+VLIGA+   G+F   IL  +    +RP+VFALSNPTSKAECTA +AY HTD + I++ GSPF PV   DK F   Q NN+YIFPG+ L +I      + +++F+ AA+ LA  V   D+ +G ++P L +I++VS+ IAV + + A+  G+A+ +PEP+DKR+++    YN  Y  S+P  + +P
Sbjct:   52 RGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVG--------NLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWP 599

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BLAST of Malic enzyme vs. Ensembl Fly
Match: Men-b (gene:FBgn0029155 transcript:FBtr0301707)

HSP 1 Score: 597.816 bits (1540), Expect = 0.000e+0
Identity = 286/556 (51.44%), Postives = 388/556 (69.78%), Query Frame = 2

Query:  182 RGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            RGID +RDPR+NKG AF L+ERQ LGIHGL P    T EEQ   C I + +  + LN++L+L  L DRNE LF++ +++N+E  +PI+YTPTVGLACQ++GL++RRP GL+IT +D+ +I  +++NWPE  V+A+ V+DGERILGLGDLGA GMGIPVGKL+LYTALAG+ P   LP+++DVGT+N DLL+DPLY+G+R KR+ G+ Y +F+D FM+AVV++YG+  LIQFEDF N NAFR L KYRN YCTFNDDIQGTASV + GL  + +I  +  KD  FLF GAG AA GIA L  +A+++ G   EEA ++IYM D+DGLL K R +                 + KD+     LAE V  IKP+VLIGA+   G+F   IL  +    +RP+VFALSNPTSKAECTA +AY HTD + I++ GSPF PV   DK F   Q NN+YIFPG+ L +I      + +++F+ AA+ LA  V   D+ +G ++P L +I++VS+ IAV + + A+  G+A+ +PEP+DKR+++    YN  Y  S+P  + +P
Sbjct:   52 RGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVG--------NLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWP 599

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BLAST of Malic enzyme vs. Ensembl Fly
Match: Men-b (gene:FBgn0029155 transcript:FBtr0085110)

HSP 1 Score: 597.816 bits (1540), Expect = 0.000e+0
Identity = 286/556 (51.44%), Postives = 388/556 (69.78%), Query Frame = 2

Query:  182 RGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            RGID +RDPR+NKG AF L+ERQ LGIHGL P    T EEQ   C I + +  + LN++L+L  L DRNE LF++ +++N+E  +PI+YTPTVGLACQ++GL++RRP GL+IT +D+ +I  +++NWPE  V+A+ V+DGERILGLGDLGA GMGIPVGKL+LYTALAG+ P   LP+++DVGT+N DLL+DPLY+G+R KR+ G+ Y +F+D FM+AVV++YG+  LIQFEDF N NAFR L KYRN YCTFNDDIQGTASV + GL  + +I  +  KD  FLF GAG AA GIA L  +A+++ G   EEA ++IYM D+DGLL K R +                 + KD+     LAE V  IKP+VLIGA+   G+F   IL  +    +RP+VFALSNPTSKAECTA +AY HTD + I++ GSPF PV   DK F   Q NN+YIFPG+ L +I      + +++F+ AA+ LA  V   D+ +G ++P L +I++VS+ IAV + + A+  G+A+ +PEP+DKR+++    YN  Y  S+P  + +P
Sbjct:   52 RGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVG--------NLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWP 599

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BLAST of Malic enzyme vs. Ensembl Fly
Match: Men (gene:FBgn0002719 transcript:FBtr0082660)

HSP 1 Score: 583.563 bits (1503), Expect = 0.000e+0
Identity = 288/553 (52.08%), Postives = 383/553 (69.26%), Query Frame = 2

Query:  185 GIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYK 1843
            G   L D R +KG AF  +ERQ LGIHG+LP  V    EQ   C   L ++D DL+++++LISL +RNE LFY V++ ++ Y +P++YTPTVGLACQ+Y L+ +  +G++I+I DK +I  +L+NWPE  V+A+VV+DGERILGLGDLGA GMGIPVGKLSLYTALAG+ PS  LP+ LDVGT+ + +L+DPLY+G+R +R  G +Y +F+D FM A V ++G+ CLIQFEDFAN NAFR+LSKYR+ +CTFNDDIQGTASV + GLL + KI   +LKDN  LF GAG AA GIA L   A+   G TEEEA ++I+M D  G++ + R       + G+T       F +  +    LAEAV++++P VLIGAA   G FN  IL ++    + PI+FALSNPTSKAECTA EAYT+T G+CI+A GSPFAPV Y +K F   Q NNSYIFPG+AL ++   +  + E +F+ AAE LA+ VS +DLA+G ++P L +I   S+ IA  I EYA+  G+A   PEP +K  +I    Y+  Y  S+P  YK
Sbjct:  218 GFTRLLDKRYSKGLAFTHEERQQLGIHGMLPYVVREPSEQVEHCRALLARLDQDLDKYMYLISLSERNERLFYNVLSSDIAYMMPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERRATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFCTFNDDIQGTASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWMVDSRGVITRDR------PKGGLT--EHKLHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQGGAFNQEILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYPGQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYPPLSSIVSCSMAIAERIVEYAYKNGLATVRPEPVNKLAFIKAQMYDLDYPRSVPATYK 762

[Close]

BLAST of Malic enzyme vs. Ensembl Fly
Match: Men (gene:FBgn0002719 transcript:FBtr0082661)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 288/553 (52.08%), Postives = 383/553 (69.26%), Query Frame = 2

Query:  185 GIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYK 1843
            G   L D R +KG AF  +ERQ LGIHG+LP  V    EQ   C   L ++D DL+++++LISL +RNE LFY V++ ++ Y +P++YTPTVGLACQ+Y L+ +  +G++I+I DK +I  +L+NWPE  V+A+VV+DGERILGLGDLGA GMGIPVGKLSLYTALAG+ PS  LP+ LDVGT+ + +L+DPLY+G+R +R  G +Y +F+D FM A V ++G+ CLIQFEDFAN NAFR+LSKYR+ +CTFNDDIQGTASV + GLL + KI   +LKDN  LF GAG AA GIA L   A+   G TEEEA ++I+M D  G++ + R       + G+T       F +  +    LAEAV++++P VLIGAA   G FN  IL ++    + PI+FALSNPTSKAECTA EAYT+T G+CI+A GSPFAPV Y +K F   Q NNSYIFPG+AL ++   +  + E +F+ AAE LA+ VS +DLA+G ++P L +I   S+ IA  I EYA+  G+A   PEP +K  +I    Y+  Y  S+P  YK
Sbjct:  214 GFTRLLDKRYSKGLAFTHEERQQLGIHGMLPYVVREPSEQVEHCRALLARLDQDLDKYMYLISLSERNERLFYNVLSSDIAYMMPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERRATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFCTFNDDIQGTASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWMVDSRGVITRDR------PKGGLT--EHKLHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQGGAFNQEILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYPGQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYPPLSSIVSCSMAIAERIVEYAYKNGLATVRPEPVNKLAFIKAQMYDLDYPRSVPATYK 758

[Close]

BLAST of Malic enzyme vs. Ensembl Zebrafish
Match: me3 (malic enzyme 3, NADP(+)-dependent, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-041111-294])

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 308/570 (54.04%), Postives = 403/570 (70.70%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            + ++RG D+ R+P +NKG AF L+ER  LGIHGLLPP   + + Q LR M +     + L++++ L++LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHDK +I  +L +WPE+ +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GVPP   LPVLLDVGTDNQ LLDDPLY+G++HKR+RGK Y D +D FMQAV +KYG  CLIQFEDFAN NAFRIL+KYRN YCTFNDDIQGTASV + G+L A KI   KL D+ F+FQGAG AA GIA LL  A+ + G    EA  +I+M D  GL+VK R     E             F  D  H K L E V+ IKPT +IG A + G F   I+  +    +RPI+FALSNPTSKAECTA + YT T+G+ I+A GSPF  V   D + F   Q NN+Y+FPG+AL +I   +R +++D+F+  AE +++ V++E LA+GR++P L TI++VS +IAV I ++A+  GIA+ +PEPKDK  +I+ H YN  Y     + Y +P +  K +++
Sbjct:   44 FTRKRGYDITRNPHLNKGMAFTLEERLQLGIHGLLPPCFLSQDVQVLRVMKSYETRSNPLDKYILLMTLQDRNEKLFYRVLTSDIEEFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDKGHIATMLNSWPEEDIKAIVVTDGERILGLGDLGGYGMGIPVGKLALYTACGGVPPQQCLPVLLDVGTDNQTLLDDPLYIGLKHKRVRGKEYDDLIDEFMQAVTDKYGMNCLIQFEDFANSNAFRILNKYRNRYCTFNDDIQGTASVAVAGILAALKITKNKLSDHKFVFQGAGEAALGIAHLLIMAMAKEGIPHAEAAQRIWMVDSKGLIVKGRSHLNHEKEE----------FAHDHPHIKTLEEVVETIKPTAIIGVAAIGGAFTEKIIKNMAANNERPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFKKVTLADGRSFYPGQGNNAYVFPGVALGVIACGVRHISDDIFLTTAEAISEMVTEEHLAEGRLYPPLKTIREVSFKIAVKIVDHAYKQGIASWYPEPKDKEAFILSHVYNSDYDSFTLDSYSWPKDAMKVQNV 603

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BLAST of Malic enzyme vs. Ensembl Zebrafish
Match: me1 (malic enzyme 1, NADP(+)-dependent, cytosolic [Source:ZFIN;Acc:ZDB-GENE-061013-438])

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 294/565 (52.04%), Postives = 403/565 (71.33%), Query Frame = 2

Query:  158 RSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++F Y K+RG DV R+P +NKG AF+L+ER  LGIHGLLPP   + + Q LR + +   + +DL+R++FL+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  +L++WPE+ ++A+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  G+PP   LPV+LDVGTD ++LL DPLY+G+RHKR+RG+ Y + +D FM+A+ ++YG +CLIQFEDFAN+NAFR+LSKYRN Y TFNDDIQGTA+V + GLL A +I   K+ D+  +FQGAG AA GI  L+  A+ + G   E+ I KI+M D  GL+VK RD            + +   F  +    K L + VKE+KPT +IG A + G F   I+  + +F   PI+FALSNPTSKAECTA + YT T+G+ I+A GSPF PV   D + F   Q NNSY+FPG+ L +   ++R + ED+F+  AE +A+ V+D+DLA+GR++P L +I++VS+++AV I EYA+   +A  HPEP+DK  +I    Y+  Y     + Y +P
Sbjct:   23 KTFVYTKKRGYDVTRNPHLNKGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRD----------HLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWP 577

[Close]

BLAST of Malic enzyme vs. Ensembl Zebrafish
Match: me1 (malic enzyme 1, NADP(+)-dependent, cytosolic [Source:ZFIN;Acc:ZDB-GENE-061013-438])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 294/565 (52.04%), Postives = 403/565 (71.33%), Query Frame = 2

Query:  158 RSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++F Y K+RG DV R+P +NKG AF+L+ER  LGIHGLLPP   + + Q LR + +   + +DL+R++FL+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  +L++WPE+ ++A+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  G+PP   LPV+LDVGTD ++LL DPLY+G+RHKR+RG+ Y + +D FM+A+ ++YG +CLIQFEDFAN+NAFR+LSKYRN Y TFNDDIQGTA+V + GLL A +I   K+ D+  +FQGAG AA GI  L+  A+ + G   E+ I KI+M D  GL+VK RD            + +   F  +    K L + VKE+KPT +IG A + G F   I+  + +F   PI+FALSNPTSKAECTA + YT T+G+ I+A GSPF PV   D + F   Q NNSY+FPG+ L +   ++R + ED+F+  AE +A+ V+D+DLA+GR++P L +I++VS+++AV I EYA+   +A  HPEP+DK  +I    Y+  Y     + Y +P
Sbjct:   23 KTFVYTKKRGYDVTRNPHLNKGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRSYELLKEDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDRSHIPTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDKEELLKDPLYIGLRHKRVRGQAYDELLDEFMKAITDRYGMKCLIQFEDFANINAFRLLSKYRNKYLTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIGELIVMAMEKEGLPREQCIKKIWMVDSKGLIVKGRD----------HLTHEKERFAHEHAQMKKLEDVVKELKPTAIIGVAAIAGAFTEEIIKAMASFNKNPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLPDGRKFYPGQGNNSYVFPGVGLGVTACEMRHIPEDVFLVTAEAIAELVTDKDLAEGRLYPPLSSIREVSIKLAVKIVEYAYKHKMATLHPEPQDKDAFIRSLIYSTDYQEFAVDSYSWP 577

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BLAST of Malic enzyme vs. Ensembl Zebrafish
Match: me2 (malic enzyme 2, NAD(+)-dependent, mitochondrial [Source:NCBI gene;Acc:445233])

HSP 1 Score: 602.053 bits (1551), Expect = 0.000e+0
Identity = 288/563 (51.15%), Postives = 401/563 (71.23%), Query Frame = 2

Query:  164 FSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            + + K++G  ++ +PR NKG AF LKERQ+LGIHGLLP  + T + QA+R   NL+K+ D L ++++L+ +Q+RNE LFY+V+ +++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +I+ IL+NWP+  VKA+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P + LPV +DVGTDN+ LL DP Y+G+  +R R + Y D +D FM+AVV+KYG+  LIQFEDF N NAFR L KYR  YCTFNDDIQGTASV + GLL A +   + L ++  LF GAG AA GIA L+  A++ESG ++ +A  KI+MFD  GLLVK R  +        T S Q      D    K   +AV  IKPT +IG +G   +F  +++  +    +RPI+FALSNPT+KAECTA +AY+ T G+C++A GSPFAPV  +D ++    Q NN+YIFPG+ALA+I+  +R +++ +F+ AA+TLA Q++DE+L+QGR++P L  I++VSL++A+ + EY +S G+A  +PEP DK  Y+    +N  Y   LP+ Y +P
Sbjct:   18 WVHTKEKGKPLMLNPRTNKGMAFTLKERQMLGIHGLLPSKIETQDTQAMRFQKNLKKMSDPLQKYIYLMGIQERNERLFYRVLMEDIEALMPIVYTPTVGLACTQYGHIFRRPKGLFISIKDQGHIRSILDNWPDTSVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPESCLPVCIDVGTDNEKLLRDPFYMGLYQRRDRSQRYDDLIDEFMEAVVDKYGQDTLIQFEDFGNHNAFRFLKKYREKYCTFNDDIQGTASVALAGLLAAQRAVGKPLTEHKVLFLGAGEAALGIANLIVMAMMESGMSQADARKKIWMFDKYGLLVKDRAYE--------TDSNQEAFVHPDPGDVKSFLDAVNVIKPTAIIGVSGAGRLFTHDVIKAMGNLNERPIIFALSNPTAKAECTAEDAYSLTQGRCLFASGSPFAPVSLEDGRILTPGQGNNAYIFPGVALAVILSGVRHISDTVFLEAAKTLADQLTDEELSQGRLYPPLSNIREVSLQMAIKVVEYVYSKGMAFRYPEPVDKESYVRSVVWNTNYDSFLPDIYDWP 572

[Close]

Query:  164 FSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            + + K++G  ++ +PR NKG AF LKERQ+LGIHGLLP  + T + QA+R   NL+K+ D L ++++L+ +Q+RNE LFY+V+ +++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +I+ IL+NWP+  VKA+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P + LPV +DVGTDN+ LL DP Y+G+  +R R + Y D +D FM+AVV+KYG+  LIQFEDF N NAFR L KYR  YCTFNDDIQGTASV + GLL A +   + L ++  LF GAG AA GIA L+  A++ESG ++ +A  KI+MFD  GLLVK R  +        T S Q      D    K   +AV  IKPT +IG +G   +F  +++  +    +RPI+FALSNPT+KAECTA +AY+ T G+C++A GSPFAPV  +D ++    Q NN+YIFPG+ALA+I+  +R +++ +F+ AA+TLA Q++DE+L+QGR++P L  I++VSL++A+ + EY +S G+A  +PEP DK  Y+    +N  Y   LP+ Y +P
Sbjct:   18 WVHTKEKGKPLMLNPRTNKGMAFTLKERQMLGIHGLLPSKIETQDTQAMRFQKNLKKMSDPLQKYIYLMGIQERNERLFYRVLMEDIEALMPIVYTPTVGLACTQYGHIFRRPKGLFISIKDQGHIRSILDNWPDTSVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPESCLPVCIDVGTDNEKLLRDPFYMGLYQRRDRSQRYDDLIDEFMEAVVDKYGQDTLIQFEDFGNHNAFRFLKKYREKYCTFNDDIQGTASVALAGLLAAQRAVGKPLTEHKVLFLGAGEAALGIANLIVMAMMESGMSQADARKKIWMFDKYGLLVKDRAYE--------TDSNQEAFVHPDPGDVKSFLDAVNVIKPTAIIGVSGAGRLFTHDVIKAMGNLNERPIIFALSNPTAKAECTAEDAYSLTQGRCLFASGSPFAPVSLEDGRILTPGQGNNAYIFPGVALAVILSGVRHISDTVFLEAAKTLADQLTDEELSQGRLYPPLSNIREVSLQMAIKVVEYVYSKGMAFRYPEPVDKESYVRSVVWNTNYDSFLPDIYDWP 572

[Close]

BLAST of Malic enzyme vs. Ensembl Xenopus
Match: abhd4 (abhydrolase domain containing 4 [Source:Xenbase;Acc:XB-GENE-921768])

HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0
Identity = 302/580 (52.07%), Postives = 408/580 (70.34%), Query Frame = 2

Query:  113 RENLK*MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            R+N     + N+  +++F Y K+RG D+ RDP +NKG AF+L+ER  LGIHGLLPP   + + Q LR + N  +   DL+R++ L+ LQDRNE LFYKV+T ++E  +P++YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  ++++WPE  +KA+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDNQ+LL+DPLY+G+RH+R+RGK Y + +D FM+AV E+YG  CLIQFEDFAN NAFR+L+ YRN YCTFNDDIQGTA+V + GLLGA +I   KL D+  LFQGAG AA GIA L+  A+ + G   + AI  I+M D  GL+VK R           + + +   F    K  K L E VK+IKPTVLIG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T G+ I+A GSPF PV   D +     Q NN+Y+FPG+AL ++   +R +++D+F+  AE +A+QVSD+ L +GR++P L TI++VS++IAV I E A+    A+  PEP++K  ++    Y+  Y   + + Y +P
Sbjct:    9 RQNQSGQQAENQDAKQTFHYTKKRGYDITRDPHLNKGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRNYERQKCDLDRYILLMGLQDRNEKLFYKVLTSDIERFMPVVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHISTVMKSWPEDNIKAVVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQECLPVMLDVGTDNQELLNDPLYIGLRHRRVRGKEYDELVDEFMKAVTERYGINCLIQFEDFANANAFRLLNMYRNKYCTFNDDIQGTAAVAVAGLLGALRITKNKLSDHTVLFQGAGEAAMGIADLIVMAMEKEGTPRDAAIKNIWMVDSKGLIVKGR----------ASLNHEKERFAHPHKEMKSLEEVVKDIKPTVLIGVAAIGGAFTEQIIKDMASFNNRPIIFALSNPTSKAECTAEQCYRLTQGRGIFASGSPFDPVTLPDGRTLYPGQGNNAYVFPGVALGVVACGVRHISDDVFLTTAEIIAQQVSDKHLEEGRLYPPLQTIQEVSVKIAVKIVENAYKDNSASVFPEPENKEAFVRSQMYSTDYDQFMVDSYSWP 578

[Close]

BLAST of Malic enzyme vs. Ensembl Xenopus
Match: abhd4 (abhydrolase domain containing 4 [Source:Xenbase;Acc:XB-GENE-921768])

HSP 1 Score: 627.091 bits (1616), Expect = 0.000e+0
Identity = 299/566 (52.83%), Postives = 402/566 (71.02%), Query Frame = 2

Query:  155 QRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +++F Y K+RG D+ RDP +NKG AF+L+ER  LGIHGLLPP   + + Q LR + N  +   DL+R++ L+ LQDRNE LFYKV+T ++E  +P++YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  ++++WPE  +KA+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDNQ+LL+DPLY+G+RH+R+RGK Y + +D FM+AV E+YG  CLIQFEDFAN NAFR+L+ YRN YCTFNDDIQGTA+V + GLLGA +I   KL D+  LFQGAG AA GIA L+  A+ + G   + AI  I+M D  GL+VK R           + + +   F    K  K L E VK+IKPTVLIG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T G+ I+A GSPF PV   D +     Q NN+Y+FPG+AL ++   +R +++D+F+  AE +A+QVSD+ L +GR++P L TI++VS++IAV I E A+    A+  PEP++K  ++    Y+  Y   + + Y +P
Sbjct:    6 KQTFHYTKKRGYDITRDPHLNKGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRNYERQKCDLDRYILLMGLQDRNEKLFYKVLTSDIERFMPVVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHISTVMKSWPEDNIKAVVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQECLPVMLDVGTDNQELLNDPLYIGLRHRRVRGKEYDELVDEFMKAVTERYGINCLIQFEDFANANAFRLLNMYRNKYCTFNDDIQGTAAVAVAGLLGALRITKNKLSDHTVLFQGAGEAAMGIADLIVMAMEKEGTPRDAAIKNIWMVDSKGLIVKGR----------ASLNHEKERFAHPHKEMKSLEEVVKDIKPTVLIGVAAIGGAFTEQIIKDMASFNNRPIIFALSNPTSKAECTAEQCYRLTQGRGIFASGSPFDPVTLPDGRTLYPGQGNNAYVFPGVALGVVACGVRHISDDVFLTTAEIIAQQVSDKHLEEGRLYPPLQTIQEVSVKIAVKIVENAYKDNSASVFPEPENKEAFVRSQMYSTDYDQFMVDSYSWP 561

[Close]

BLAST of Malic enzyme vs. Ensembl Xenopus
Match: abhd4 (abhydrolase domain containing 4 [Source:Xenbase;Acc:XB-GENE-921768])

HSP 1 Score: 600.897 bits (1548), Expect = 0.000e+0
Identity = 290/546 (53.11%), Postives = 388/546 (71.06%), Query Frame = 2

Query:  218 KGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHR-DLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +G AF+L+ER  LGIHGLLPP   + + Q LR + N  +   DL+R++ L+ LQDRNE LFYKV+T ++E  +P++YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  ++++WPE  +KA+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDNQ+LL+DPLY+G+RH+R+RGK Y + +D FM+AV E+YG  CLIQFEDFAN NAFR+L+ YRN YCTFNDDIQGTA+V + GLLGA +I   KL D+  LFQGAG AA GIA L+  A+ + G   + AI  I+M D  GL+VK R  L+ ++ R           F    K  K L E VK+IKPTVLIG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T G+ I+A GSPF PV   D +     Q NN+Y+FPG+AL ++   +R +++D+F+  AE +A+QVSD+ L +GR++P L TI++VS++IAV I E A+    A+  PEP++K  ++    Y+  Y   + + Y +P
Sbjct:    1 RGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRNYERQKCDLDRYILLMGLQDRNEKLFYKVLTSDIERFMPVVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHISTVMKSWPEDNIKAVVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQECLPVMLDVGTDNQELLNDPLYIGLRHRRVRGKEYDELVDEFMKAVTERYGINCLIQFEDFANANAFRLLNMYRNKYCTFNDDIQGTAAVAVAGLLGALRITKNKLSDHTVLFQGAGEAAMGIADLIVMAMEKEGTPRDAAIKNIWMVDSKGLIVKGRASLNHEKER-----------FAHPHKEMKSLEEVVKDIKPTVLIGVAAIGGAFTEQIIKDMASFNNRPIIFALSNPTSKAECTAEQCYRLTQGRGIFASGSPFDPVTLPDGRTLYPGQGNNAYVFPGVALGVVACGVRHISDDVFLTTAEIIAQQVSDKHLEEGRLYPPLQTIQEVSVKIAVKIVENAYKDNSASVFPEPENKEAFVRSQMYSTDYDQFMVDSYSWP 535

[Close]

BLAST of Malic enzyme vs. Ensembl Xenopus
Match: abhd4 (abhydrolase domain containing 4 [Source:Xenbase;Acc:XB-GENE-921768])

HSP 1 Score: 600.127 bits (1546), Expect = 0.000e+0
Identity = 290/548 (52.92%), Postives = 389/548 (70.99%), Query Frame = 2

Query:  212 INKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHR-DLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++ G AF+L+ER  LGIHGLLPP   + + Q LR + N  +   DL+R++ L+ LQDRNE LFYKV+T ++E  +P++YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  ++++WPE  +KA+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDNQ+LL+DPLY+G+RH+R+RGK Y + +D FM+AV E+YG  CLIQFEDFAN NAFR+L+ YRN YCTFNDDIQGTA+V + GLLGA +I   KL D+  LFQGAG AA GIA L+  A+ + G   + AI  I+M D  GL+VK R  L+ ++ R           F    K  K L E VK+IKPTVLIG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T G+ I+A GSPF PV   D +     Q NN+Y+FPG+AL ++   +R +++D+F+  AE +A+QVSD+ L +GR++P L TI++VS++IAV I E A+    A+  PEP++K  ++    Y+  Y   + + Y +P
Sbjct:    1 MSPGMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRNYERQKCDLDRYILLMGLQDRNEKLFYKVLTSDIERFMPVVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHISTVMKSWPEDNIKAVVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQECLPVMLDVGTDNQELLNDPLYIGLRHRRVRGKEYDELVDEFMKAVTERYGINCLIQFEDFANANAFRLLNMYRNKYCTFNDDIQGTAAVAVAGLLGALRITKNKLSDHTVLFQGAGEAAMGIADLIVMAMEKEGTPRDAAIKNIWMVDSKGLIVKGRASLNHEKER-----------FAHPHKEMKSLEEVVKDIKPTVLIGVAAIGGAFTEQIIKDMASFNNRPIIFALSNPTSKAECTAEQCYRLTQGRGIFASGSPFDPVTLPDGRTLYPGQGNNAYVFPGVALGVVACGVRHISDDVFLTTAEIIAQQVSDKHLEEGRLYPPLQTIQEVSVKIAVKIVENAYKDNSASVFPEPENKEAFVRSQMYSTDYDQFMVDSYSWP 537

[Close]

BLAST of Malic enzyme vs. Ensembl Xenopus
Match: abhd4 (abhydrolase domain containing 4 [Source:Xenbase;Acc:XB-GENE-921768])

HSP 1 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 290/545 (53.21%), Postives = 387/545 (71.01%), Query Frame = 2

Query:  221 GTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHR-DLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            G AF+L+ER  LGIHGLLPP   + + Q LR + N  +   DL+R++ L+ LQDRNE LFYKV+T ++E  +P++YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  ++++WPE  +KA+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDNQ+LL+DPLY+G+RH+R+RGK Y + +D FM+AV E+YG  CLIQFEDFAN NAFR+L+ YRN YCTFNDDIQGTA+V + GLLGA +I   KL D+  LFQGAG AA GIA L+  A+ + G   + AI  I+M D  GL+VK R  L+ ++ R           F    K  K L E VK+IKPTVLIG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T G+ I+A GSPF PV   D +     Q NN+Y+FPG+AL ++   +R +++D+F+  AE +A+QVSD+ L +GR++P L TI++VS++IAV I E A+    A+  PEP++K  ++    Y+  Y   + + Y +P
Sbjct:   20 GMAFSLEERLQLGIHGLLPPCFLSQDVQVLRVLRNYERQKCDLDRYILLMGLQDRNEKLFYKVLTSDIERFMPVVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHISTVMKSWPEDNIKAVVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQECLPVMLDVGTDNQELLNDPLYIGLRHRRVRGKEYDELVDEFMKAVTERYGINCLIQFEDFANANAFRLLNMYRNKYCTFNDDIQGTAAVAVAGLLGALRITKNKLSDHTVLFQGAGEAAMGIADLIVMAMEKEGTPRDAAIKNIWMVDSKGLIVKGRASLNHEKER-----------FAHPHKEMKSLEEVVKDIKPTVLIGVAAIGGAFTEQIIKDMASFNNRPIIFALSNPTSKAECTAEQCYRLTQGRGIFASGSPFDPVTLPDGRTLYPGQGNNAYVFPGVALGVVACGVRHISDDVFLTTAEIIAQQVSDKHLEEGRLYPPLQTIQEVSVKIAVKIVENAYKDNSASVFPEPENKEAFVRSQMYSTDYDQFMVDSYSWP 553

[Close]

BLAST of Malic enzyme vs. Ensembl Mouse
Match: Me3 (malic enzyme 3, NADP(+)-dependent, mitochondrial [Source:MGI Symbol;Acc:MGI:1916679])

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 299/569 (52.55%), Postives = 400/569 (70.30%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M        DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK +I  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+RG+ Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   +L +++F+FQGAG AA GIA LL  AL + G  + EAI KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPIVFALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D + F   Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IAV + +YA+   +A+++PEPKDK  ++    Y P Y     + Y +P E    + +
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYENQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHIATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGEEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNRLSNHVFVFQGAGEAAMGIAHLLVMALEKEGIPKTEAIKKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIVFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFTPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAVKVLDYAYKHNLASYYPEPKDKEAFVKSLIYTPDYDSFSLDTYSWPKEAMSVQKV 604

[Close]

BLAST of Malic enzyme vs. Ensembl Mouse
Match: Me1 (malic enzyme 1, NADP(+)-dependent, cytosolic [Source:MGI Symbol;Acc:MGI:97043])

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 292/568 (51.41%), Postives = 389/568 (68.49%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            +  QRG  + RDP +NK  AF L+ERQ L IHGLLPP + + E Q LR + N  +++ D +R+L L+ LQDRNE LFY V+  +VE  +PI+YTPTVGLACQ+Y L FR+PRGL+I+IHDK +I  +L  WPE +VKA+VV+DGERILGLGDLG  GMGIPVGKL+LYTA  GV P   LP+ LDVGT+N++LL DPLY+G+RH+R+RG  Y  F+D FM+A   KYG  CLIQFEDFAN NAFR+L+KYRN YCTFNDDIQGTASV + GLL A +I   KL D   LFQGAG AA GIA L+  A+ + G ++E A  KI++ D  GL+VK R           + + +  +F  + +  K L   V++IKPT LIG A + G F   IL  +  F +RPI+FALSNPTSKAEC+A + Y  T G+ I+A GSPF PV   D +     Q NNSY+FPG+AL ++   +R + + +F+  AE +++QVSD+ L +GR++P L TI+ VSL+IAV I + A+   +A  +PEP++K E++    Y+  Y   LP+ Y +P E+ K +
Sbjct:   10 HTHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIISQELQVLRIIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEKEGLSKENARKKIWLVDSKGLIVKGR----------ASLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKVTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDKHLQEGRLYPPLNTIRGVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWPAEVQKIQ 567

[Close]

BLAST of Malic enzyme vs. Ensembl Mouse
Match: Me2 (malic enzyme 2, NAD(+)-dependent, mitochondrial [Source:MGI Symbol;Acc:MGI:2147351])

HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0
Identity = 277/561 (49.38%), Postives = 400/561 (71.30%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++K++G  ++ +PR NKG AF L+ERQ+LG+ GLLPP + T + QALR   NL+K+   L ++++++ +Q+RNE LFY+++ D++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +++ I++NWPE  VKA+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P   LPV +DVGTDN  LL DP Y+G+  KR R ++Y D MD FM+A+ ++YGR  LIQFEDF N NAFR L KY+  YCTFNDDIQGTA+V + GLL A ++ N+ + ++  LF GAG AA GIA L+  +++ESG +EEEA  KI+MFD  GLLVK R       +     +   +I            +AV ++KP+V+IG AG   +F   ++  + +  +RPI+FALSNPT++AECTA +AYT T+G+C++A GSPF PV  +D +VF   Q NN+YIFPG+ALA+I+ + R +++ +F+ AA+ L  Q++D +LAQGR++P+L  I++VS  IA+ +AEY ++  +A  +PEP+DK  Y+ +  +   Y+  LP+ Y +P
Sbjct:   20 HLKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFYMGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTFNDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSEEEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESI-------PATFEDAVNKLKPSVIIGVAGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFEPVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELAQGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLLPDVYDWP 573

[Close]

BLAST of Malic enzyme vs. Ensembl Mouse
Match: Me1 (malic enzyme 1, NADP(+)-dependent, cytosolic [Source:MGI Symbol;Acc:MGI:97043])

HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 284/549 (51.73%), Postives = 378/549 (68.85%), Query Frame = 2

Query:  227 AFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            AF L+ERQ L IHGLLPP + + E Q LR + N  +++ D +R+L L+ LQDRNE LFY V+  +VE  +PI+YTPTVGLACQ+Y L FR+PRGL+I+IHDK +I  +L  WPE +VKA+VV+DGERILGLGDLG  GMGIPVGKL+LYTA  GV P   LP+ LDVGT+N++LL DPLY+G+RH+R+RG  Y  F+D FM+A   KYG  CLIQFEDFAN NAFR+L+KYRN YCTFNDDIQGTASV + GLL A +I   KL D   LFQGAG AA GIA L+  A+ + G ++E A  KI++ D  GL+VK R           + + +  +F  + +  K L   V++IKPT LIG A + G F   IL  +  F +RPI+FALSNPTSKAEC+A + Y  T G+ I+A GSPF PV   D +     Q NNSY+FPG+AL ++   +R + + +F+  AE +++QVSD+ L +GR++P L TI+ VSL+IAV I + A+   +A  +PEP++K E++    Y+  Y   LP+ Y +P E+ K +
Sbjct:    9 AFTLEERQQLNIHGLLPPCIISQELQVLRIIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEKEGLSKENARKKIWLVDSKGLIVKGR----------ASLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKVTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDKHLQEGRLYPPLNTIRGVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYPWPAEVQKIQ 547

[Close]

BLAST of Malic enzyme vs. Ensembl Mouse
Match: Me3 (malic enzyme 3, NADP(+)-dependent, mitochondrial [Source:MGI Symbol;Acc:MGI:1916679])

HSP 1 Score: 87.0409 bits (214), Expect = 1.334e-20
Identity = 36/54 (66.67%), Postives = 47/54 (87.04%), Query Frame = 2

Query:  344 DLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPR 505
            DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPR
Sbjct:    8 DLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPR 61

[Close]

BLAST of Malic enzyme vs. UniProt/SwissProt
Match: sp|P28227|MAOX_ANAPL (NADP-dependent malic enzyme OS=Anas platyrhynchos OX=8839 GN=ME1 PE=1 SV=1)

HSP 1 Score: 631.713 bits (1628), Expect = 0.000e+0
Identity = 305/565 (53.98%), Postives = 398/565 (70.44%), Query Frame = 2

Query:  179 QRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            +RG +VLRDP +NKG AF L+ERQ L IHGLLPP     + Q    + N  ++  DL+R++ L+SLQDRNE LFYKV+T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  +L++WPE ++KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDN+ LL DPLY+G+RHKRIRG+ Y D +D FM+AV  +YG  CLIQFEDFAN NAFR+L KYRN YCTFNDDIQGTASV + GLL A +I   +L D+  LFQGAG AA GIA L+  A+ + G ++E A+ +I+M D  GL+VK R           + + + T F  +    K L + VK+IKP+VLIG A + G F   IL  +  F  RPI+FALSNPTSKAECTA + Y +T+G+ I+A GSPF PV   + K     Q NNSY+FPG+AL +I   ++ + ED+F+  AE +A+QVS+E+L +GR++P L TI+ VSL+IAV IAE A+    A+ +P+PKD   +I    Y+  Y   + + Y +P E  K K
Sbjct:    2 KRGYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDVQVFSILKNFERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHIATMLKSWPESVIKAIVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVKPHECLPVMLDVGTDNEALLKDPLYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQFEDFANANAFRLLHKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANLIVMAMEKEGVSKEAAVKRIWMVDSKGLIVKGR----------ASLTAEKTRFAHEHAEMKNLEDIVKDIKPSVLIGVAAIGGAFTKEILQDMAAFNKRPIIFALSNPTSKAECTAEQCYKYTEGRGIFASGSPFDPVTLPNGKTLYPGQGNNSYVFPGVALGVIACGLKHIGEDVFLTTAEVIAEQVSEENLQEGRLYPPLVTIQHVSLKIAVRIAEEAYRNNTASTYPQPKDLEAFIQSQIYSTDYNSFVADSYTWPEEAMKVK 556

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BLAST of Malic enzyme vs. UniProt/SwissProt
Match: sp|P40927|MAOX_COLLI (NADP-dependent malic enzyme OS=Columba livia OX=8932 GN=ME1 PE=1 SV=1)

HSP 1 Score: 625.165 bits (1611), Expect = 0.000e+0
Identity = 302/565 (53.45%), Postives = 397/565 (70.27%), Query Frame = 2

Query:  179 QRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHY-KDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            ++G +VLRDP +NKG AF L+ERQ L IHGLLPP     + Q    + N  ++  DL+R++ L+SLQDRNE LFYKV+T ++E  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHD+ +I  +L++WPE ++KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPV+LDVGTDN+ LL DPLY+G+RHKRIRG+ Y D +D FM+AV  +YG  CLIQFEDFAN NAFR+L KYRN YCTFNDDIQGTASV + GLL A +I   +L D+  LFQGAG AA GIA L+  A+ + G ++EEAI +I+M D  GL+VK R           + +P+   F  +    K L + VK+IKPTVLIG A + G F   IL  +  F  RPI+FALSNPTSKAECTA + Y +T+G+ I+A GSPF PV     +     Q NNSY+FPG+AL +I   ++ + +D+F+  AE +A++VS+E+L +GR++P L TI+ VSL+IAV IA+ A+    A+ +P+P+D   +I    Y+  Y   + + Y +P E  K K
Sbjct:    2 KKGYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDAQVYSILKNFERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQHYGLAFRRPRGLFITIHDRGHIATMLQSWPESVIKAIVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVKPHQCLPVMLDVGTDNETLLKDPLYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQFEDFANANAFRLLHKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANLIVMAMQKEGVSKEEAIKRIWMVDSKGLIVKGR----------ASLTPEKEHFAHEHCEMKNLEDIVKDIKPTVLIGVAAIGGAFTQQILQDMAAFNKRPIIFALSNPTSKAECTAEQLYKYTEGRGIFASGSPFDPVTLPSGQTLYPGQGNNSYVFPGVALGVISCGLKHIGDDVFLTTAEVIAQEVSEENLQEGRLYPPLVTIQQVSLKIAVRIAKEAYRNNTASTYPQPEDLEAFIRSQVYSTDYNCFVADSYTWPEEAMKVK 556

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BLAST of Malic enzyme vs. UniProt/SwissProt
Match: sp|P48163|MAOX_HUMAN (NADP-dependent malic enzyme OS=Homo sapiens OX=9606 GN=ME1 PE=1 SV=1)

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 296/568 (52.11%), Postives = 395/568 (69.54%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFK 1870
            +  QRG  + R+P +NK  AF L+ERQ L IHGLLPP  N+ E Q LR + N   ++ D +R+L L+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+Y L+FR+PRGL+ITIHD+ +I  +L  WPE ++KA+VV+DGERILGLGDLG  GMGIPVGKL+LYTA  G+ P   LPV+LDVGT+N++LL DPLY+G+R +R+RG  Y DF+D FM+AV  KYG  CLIQFEDFANVNAFR+L+KYRN YCTFNDDIQGTASV + GLL A +I   KL D   LFQGAG AA GIA L+  AL + G  +E+AI KI++ D  GL+VK R           + + +   F  + +  K L   V+EIKPT LIG A + G F+  IL  +  F +RPI+FALSNPTSKAEC+A + Y  T G+ I+A GSPF PV   + +     Q NNSY+FPG+AL ++   +R +T+++F+  AE +A+QVSD+ L +GR++P L TI+DVSL+IA  I + A+    A  +PEP++K  ++    Y+  Y   LP+ Y +P E+ K +
Sbjct:   10 HTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGR----------ASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQ 567

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BLAST of Malic enzyme vs. UniProt/SwissProt
Match: sp|Q8BMF3|MAON_MOUSE (NADP-dependent malic enzyme, mitochondrial OS=Mus musculus OX=10090 GN=Me3 PE=1 SV=2)

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 299/569 (52.55%), Postives = 400/569 (70.30%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M        DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK +I  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+RG+ Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   +L +++F+FQGAG AA GIA LL  AL + G  + EAI KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPIVFALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D + F   Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IAV + +YA+   +A+++PEPKDK  ++    Y P Y     + Y +P E    + +
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYENQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHIATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGEEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNRLSNHVFVFQGAGEAAMGIAHLLVMALEKEGIPKTEAIKKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIVFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFTPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAVKVLDYAYKHNLASYYPEPKDKEAFVKSLIYTPDYDSFSLDTYSWPKEAMSVQKV 604

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BLAST of Malic enzyme vs. UniProt/SwissProt
Match: sp|Q16798|MAON_HUMAN (NADP-dependent malic enzyme, mitochondrial OS=Homo sapiens OX=9606 GN=ME3 PE=2 SV=2)

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 298/569 (52.37%), Postives = 400/569 (70.30%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            +K+RG DV R+P +NKG AF L+ER  LGIHGL+PP   + + Q LR M    +   DL++++ L++LQDRNE LFY+V+T +VE  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHDK ++  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GV P   LPVLLDVGT+N++LL DPLY+G++H+R+ GK Y D +D FMQAV +K+G  CLIQFEDFAN NAFR+L+KYRN YC FNDDIQGTASV + G+L A +I   KL +++F+FQGAG AA GIA LL  AL + G  + EA  KI+M D  GL+VK R             + +  +F +D      L E V+ +KPT +IG A + G F   IL  + +F +RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF  V  +D K FI  Q NN+Y+FPG+AL +I   IR + +++F+  AE +A++VS++ L+QGR++P L TI+DVSL IA+ + +YA+   +A+++PEPKDK  ++    Y P Y     + Y +P E    + +
Sbjct:   46 LKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRS----------HLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV 604

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BLAST of Malic enzyme vs. TrEMBL
Match: A0A3Q0KLC0 (Malic enzyme OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 334/569 (58.70%), Postives = 434/569 (76.27%), Query Frame = 2

Query:  158 RSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVK-DMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFA----PVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            R   +++  GIDV+RDPR N+GTAF L ERQLLGIHGLLPP V T+++Q  + M NL+ ++D+L R+++L SLQDRNE LFYK+V ++VEYC+P+IYTPTVGLACQ+YG++FRRPRGLYIT+HD+ +I  IL NWPE IVKA+V +DGERILGLGDLGAYGMGIP+GKLSLYTALAGV P + LP+LLDVGTDNQ+LL+DP+Y G+RHKRIR + Y + ++ FMQAV E+YG  CL+QFEDF N NAFR+L +Y++ YCTFNDDIQGTA+VT+ GLL A +I  RKL DNI+LF GAG AATGIA LL  +L  +G  E+EA+SKIYMFD DGLL   R    QE     + +  N +F + D+++ + L +AV+++KPTV+IGA+G  G+F   IL  +   ++RPI+F+LSNPTSK+ECTA  AY  T+G+C++A GSPF      V  K+  F   QCNNSYIFPG+ LAI + QIRPV ++LF+ AAE LA QV ++DL  GRVFP L  I++VSL IAV +AEYA+S GI ++ P+PKD   Y+ +  Y+P Y+P +P  Y +P
Sbjct:   32 RDSDFIRLHGIDVVRDPRTNRGTAFTLNERQLLGIHGLLPPSVLTLDQQVSKMMANLKNMNDNLQRYVYLTSLQDRNEALFYKLVIEHVEYCMPLIYTPTVGLACQRYGVVFRRPRGLYITVHDRHHIPEILNNWPEPIVKAIVFTDGERILGLGDLGAYGMGIPIGKLSLYTALAGVHPKHCLPILLDVGTDNQELLNDPVYTGIRHKRIRDERYDELIENFMQAVAERYGNHCLVQFEDFGNHNAFRLLERYQDSYCTFNDDIQGTAAVTVAGLLAAGRITKRKLTDNIYLFVGAGEAATGIAQLLATSLQLNGLDEKEALSKIYMFDKDGLLTHSR----QEG----SLTDHNKVFARDDIENIRKLEDAVEKLKPTVIIGASGQGGIFTDAILQQMVKNSERPIIFSLSNPTSKSECTAERAYRITEGRCVFAGGSPFNDVLLKVSGKEVHFQPGQCNNSYIFPGVGLAITLCQIRPVLQELFVIAAECLAGQVDEKDLDVGRVFPPLRDIRNVSLAIAVRVAEYAYSKGICHYEPQPKDIELYLSQKMYDPNYIPVMPEVYDWP 592

[Close]

BLAST of Malic enzyme vs. TrEMBL
Match: A0A0R3SQ67 (Malic enzyme OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7193 PE=3 SV=1)

HSP 1 Score: 682.945 bits (1761), Expect = 0.000e+0
Identity = 338/582 (58.08%), Postives = 429/582 (73.71%), Query Frame = 2

Query:  137 SFNKLFQ-RSF-SYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHY----KDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWK 1864
            S  + FQ R F S +   GI VLR+P  NKGTAF ++ERQLLGIHGLLPP V+ ++ Q  + + NL++++D+L R++FLI LQDRNE+LFYKVVT++ EYC+P+IYTPTVGLACQ+YGL+FRRPRGLYITIHD+  I  IL+NWPE+IV+A+VV+DGERILGLGDLGAYGMGIPVGKLSLY+ALAGV P + LP++LDVGTDNQ+L++DPLY+GV HKRIR   Y + +D FM+AVV +YGR CLIQFEDF N NAFRIL +YRNDYCTFNDDIQGTASVT+ GL+ A ++  R+L D+ +LF GAG AA+GIA LLK A++  G  E EAI++IYMFD DGLLV+ R     +  +G       T    DM+  K L E VK+IKPT LIGA+GV G+F   IL  +    DRPI+FALSNPT KAECTA  AY+ T G C++A GSPF  V      + K F   QCNNSYIFPG+ LAI   ++RP+ ++ F  AA+ LA+QVS+ DLA GRVFP L  I+ VSL IA  ++E+A+   +A+  P+P+D   Y+    Y PTY  +LPN  ++P E  +
Sbjct:    5 SLVQFFQKRCFASEIHSYGIGVLRNPATNKGTAFTIRERQLLGIHGLLPPAVSNLDAQVKKMINNLKRLEDNLQRYMFLIGLQDRNENLFYKVVTEHTEYCMPLIYTPTVGLACQRYGLVFRRPRGLYITIHDRYNIDDILKNWPEKIVRAIVVTDGERILGLGDLGAYGMGIPVGKLSLYSALAGVNPRHCLPIVLDVGTDNQELINDPLYMGVPHKRIRDARYDELLDNFMRAVVRRYGRNCLIQFEDFGNQNAFRILERYRNDYCTFNDDIQGTASVTVAGLIAAGRLTGRRLCDDTYLFVGAGEAASGIAHLLKTAMVMHGTPEHEAINRIYMFDKDGLLVQDRPEGNIDEHNG-------TFARTDMEPMKNLEEIVKKIKPTALIGASGVGGLFTHRILQQMKKNIDRPIIFALSNPTDKAECTAEAAYSTTGGACVFASGSPFGEVTIDVGGQRKTFKPGQCNNSYIFPGVGLAITACKLRPIPDECFAVAADCLARQVSESDLALGRVFPPLSQIRPVSLAIAHEVSEFAYDENVAHFMPKPEDLESYLQSRIYVPTYDDALPNYTEWPEEALR 579

[Close]

BLAST of Malic enzyme vs. TrEMBL
Match: A0A0V0J3Q3 (Malic enzyme OS=Schistocephalus solidus OX=70667 GN=TR150162 PE=3 SV=1)

HSP 1 Score: 679.863 bits (1753), Expect = 0.000e+0
Identity = 346/581 (59.55%), Postives = 421/581 (72.46%), Query Frame = 2

Query:  131 MASFNKLFQRSFSY-VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVK-DMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYK----DKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*E 1855
            +A F  L +R +S  V+  GI VLR+P  NKGTAF + ERQLLGIHGLLPP V+  + Q  R M NLR+++DDL R++FLI +QDRNE LFYKVVT+  EYC+P+IYTPTVGLACQ+YGL+FRRPRGLYITIHD+  I  IL NWPE IV+A+VV+DGERILGLGDLGAYGMGIPVGKLSLY+ALAGV P   LP++LDVG+DNQDLL DP YLGVRH R+R   Y + +D FM+AVV +YG  CLIQFEDF N NAFRIL KYR+DYCTFNDDIQGTASVT+ GL+ A ++  R+L D+I+LF GAG AATGIA LLK +++  G  EE+AI +IYMFD DGLLV        E R        N  F + D++    L E VK++KPTVLIGA+GV G+F   IL  +T  +D+PI+FALSNPT KAECTA  AY  T G C++A GSPF  V       +K F   QCNNSYIFPG+ LAI   ++RP+ E+ F  AAE LA+QV  EDLA GRVFP L  I+ VSL IAV +AEYA++A IA+  P+P++   YI    Y P Y  +LP  Y +P E
Sbjct:    3 LALFQGLLRRLYSTEVRAHGIGVLRNPSTNKGTAFPIHERQLLGIHGLLPPVVSDSKLQVKRMMANLRRLEDDLQRYIFLIGMQDRNETLFYKVVTEYTEYCMPLIYTPTVGLACQRYGLVFRRPRGLYITIHDRFNIDDILRNWPEPIVRAIVVTDGERILGLGDLGAYGMGIPVGKLSLYSALAGVNPRQCLPIVLDVGSDNQDLLSDPYYLGVRHNRVRDSRYDELLDNFMRAVVRRYGNNCLIQFEDFGNRNAFRILQKYRDDYCTFNDDIQGTASVTVAGLIAAGRLTGRQLNDDIYLFVGAGEAATGIAHLLKTSMVMHGVNEEDAIKRIYMFDKDGLLV--------EGRPKGALDDHNKTFARDDIEPMNNLEEIVKKVKPTVLIGASGVGGLFTHRILQQMTKNSDKPIIFALSNPTDKAECTAEMAYKVTQGNCVFASGSPFGDVTINVGGTEKTFRPGQCNNSYIFPGVGLAITACKLRPIAEEAFAVAAECLAEQVKPEDLALGRVFPPLSQIRPVSLAIAVRVAEYAYNANIAHQIPKPENLEAYICGQMYQPEYEAALPECYDWPAE 575

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BLAST of Malic enzyme vs. TrEMBL
Match: A0A5K3F5P2 (Malic enzyme OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 334/577 (57.89%), Postives = 420/577 (72.79%), Query Frame = 2

Query:  137 SFNKLFQRSF--SYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHY----KDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            S  + F + F  S +   GI V+R+P  NKGTAF ++ERQLLGIHGLLPP V+ +E Q+ + + NL++++D+L R++FLI LQDRNE LFYKVVT++ EYCLP+IYTPTVGLACQ+YGL+FRRPRGLYIT+HD+  I  IL+NWPE IV+A+VV+DGERILGLGDLGAYGMGIPVGKLSLY+ALAGV P + LPV+LDVG+DN+DLL+DP Y+GV HKRIR   Y + +D FM+AVV +YG  CLIQFEDF N NAFRIL +YRNDYCTFNDDIQGTASVT+ GL+ A ++  R + D+ +LF GAG AA+GIA LLK A++  G +E+EAI++IYM+D DGLLV+ R     +  +G       T    D++  + L E VK+ KPT LIGA+GV G+F   IL  +    DRPI+FALSNPT KAECTA  AY  T G C++A GSPF  V      + K F   QCNNSYIFPG+ LAI   ++RP+ ++ F  AAE LA QV DEDLA GRVFP L  I+ VSL IA  +AEYA+ A IA+  P+P +   YI    Y P+Y  +LP  Y +P
Sbjct:    5 SLVQFFSKRFFASEIHSYGIGVIRNPSTNKGTAFTIRERQLLGIHGLLPPAVSDLEAQSKKMISNLKRLEDNLQRYIFLIGLQDRNETLFYKVVTEHTEYCLPLIYTPTVGLACQRYGLVFRRPRGLYITVHDRYNIDDILKNWPEPIVRAIVVTDGERILGLGDLGAYGMGIPVGKLSLYSALAGVNPRHCLPVVLDVGSDNKDLLNDPFYMGVAHKRIRDSRYDELLDNFMRAVVRRYGNNCLIQFEDFGNRNAFRILERYRNDYCTFNDDIQGTASVTVAGLIAAGRLTGRSISDDTYLFVGAGEAASGIAHLLKTAMMMHGVSEDEAINRIYMYDKDGLLVQGRPEGNIDDHNG-------TFARTDVEPMRNLEEIVKKFKPTALIGASGVGGLFTHRILQQMKKNVDRPIIFALSNPTDKAECTAEAAYKITQGACVFASGSPFGEVTVDVAGQQKTFKPGQCNNSYIFPGVGLAITACKLRPIPDECFAIAAECLADQVKDEDLALGRVFPPLSQIRPVSLAIAHRVAEYAYEADIAHLIPKPNNLVSYIQNQMYVPSYDNALPEFYSWP 574

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BLAST of Malic enzyme vs. TrEMBL
Match: A0A183ME64 (Malic enzyme OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS14339 PE=3 SV=1)

HSP 1 Score: 675.626 bits (1742), Expect = 0.000e+0
Identity = 331/566 (58.48%), Postives = 430/566 (75.97%), Query Frame = 2

Query:  167 SYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKI-LAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAP----VHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++++  GIDV+RDPR N+GTAF L ERQLLGIHGLLPP V T+++Q  + M NL+ ++D+L R+++L SLQDRNE LFYK+V ++VEYC+P+IYTPTVGLACQ+YG++FRRPRGLYIT+HD+ +I  IL NWPE IVKA+V +DGERILGLGDLGAYGMGIP+GKLSLYTALAGV P + LP+LLDVGTDNQ+LL+DP+Y G+RHKRIR + Y + ++ FMQAV E+YG  CL+QFEDF N NAFR+L +Y++ YCTFNDDIQGTA+V + GLL A +I  RKL DNI+LF GAG AATGIA LL  +L  +G  E+EA+SK+YMFD DGLL          +R   + +  N +F +D   +   L +AVK +KPTV+IGA+G  G+F   IL  +T  ++RPI+F+LSNPTSK+ECTA  AY  T+G+C++A GSPF      V  K+  F   QCNNSYIFPG+ LAI + QIRPV ++LF+ AAE LA QV ++DL  GRVFP L  I++VSL IAV +A+YA+S GI ++ P+PKD   Y+ +  Y+P YLP +P+ Y +P
Sbjct:   30 NFIRLHGIDVVRDPRTNRGTAFTLNERQLLGIHGLLPPSVLTLDQQVSKMMANLKNMNDNLQRYVYLTSLQDRNEALFYKLVIEHVEYCMPLIYTPTVGLACQRYGVVFRRPRGLYITVHDRHHIPAILNNWPEPIVKAIVFTDGERILGLGDLGAYGMGIPIGKLSLYTALAGVHPKHCLPILLDVGTDNQELLNDPVYTGIRHKRIRDERYDELIENFMQAVTERYGNHCLVQFEDFGNHNAFRLLERYQDSYCTFNDDIQGTAAVAVAGLLAAGRITKRKLTDNIYLFVGAGEAATGIAQLLATSLQLNGLDEKEALSKMYMFDKDGLLT--------HSRQEGSLTDHNKVFARDDTENICKLEDAVKLLKPTVIIGASGQGGIFTDAILQQMTKNSERPIIFSLSNPTSKSECTAERAYKITEGRCVFAGGSPFNDVLLNVSGKEVHFQPGQCNNSYIFPGVGLAITLCQIRPVLQELFVIAAECLADQVDEKDLDVGRVFPPLRDIRNVSLAIAVHVAKYAYSKGICHYEPQPKDIELYLSQKMYDPNYLPVMPDLYDWP 587

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BLAST of Malic enzyme vs. Ensembl Cavefish
Match: me1 (NADP-dependent malic enzyme [Source:NCBI gene;Acc:103030051])

HSP 1 Score: 627.476 bits (1617), Expect = 0.000e+0
Identity = 296/567 (52.20%), Postives = 401/567 (70.72%), Query Frame = 2

Query:  158 RSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*E 1855
            ++F + K+RG D+ R+P +NKG AF+L+ER  LGIHGLLPP   + + Q LR + N     DDL+R++FL+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHD+ +I  +L++WPE+ ++A+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GVPP + LPV+LDVGTDN++LL DPLY+G+RH+R+RG  Y + ++ FM+AV ++YG  CLIQFEDFANVNAFR+L+KYRN Y TFNDDIQGTASV + GLL A ++   K+ D+  +FQGAG AA GIA L+  A+ + G +  E + KI+M D  GL+VK RD            + +   F       + L + V+E+KPT +IG A V G F   I+  +  F  RPI+FALSNPTSKAECTA + YT T+G+ I+A GSPF PV   D + F   Q NN+Y+FPG+ L +    +R ++ED+F+  AET+A+ V+++DLA+GR++P L +I+ VS +IAV I EYA+   IA H+PEP DK  ++    +N  Y     + Y +P E
Sbjct:   23 KTFVHTKKRGYDITRNPHLNKGMAFSLEERLQLGIHGLLPPAFLSQDVQLLRVLRNYELKKDDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDRHHISTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVPPQHCLPVMLDVGTDNEELLKDPLYIGLRHRRVRGSAYDELVEEFMKAVTDRYGMDCLIQFEDFANVNAFRLLTKYRNKYLTFNDDIQGTASVAVAGLLAALRVTKSKMSDHTIVFQGAGEAAMGIAELIAMAMEKEGLSHAECLKKIWMVDSKGLIVKGRD----------HLTAEKERFAHQHSQMRKLEDVVRELKPTAIIGVAAVAGAFTERIIRDMAEFNQRPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLADGRRFYPGQGNNAYVFPGVGLGVTACAMRNISEDIFLITAETIAQMVTEKDLAEGRLYPPLPSIQHVSFKIAVKIVEYAYQHKIATHYPEPADKEAFVRSLIFNTNYDNFAVDSYGWPDE 579

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BLAST of Malic enzyme vs. Ensembl Cavefish
Match: me3 (NADP-dependent malic enzyme-like [Source:NCBI gene;Acc:103029083])

HSP 1 Score: 601.668 bits (1550), Expect = 0.000e+0
Identity = 305/569 (53.60%), Postives = 402/569 (70.65%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
             K+RG D+ R+P +NKG AF L+ER  LGIHGLLPP   + + Q LR M +     + L++++ L++LQDRNE LFY+++T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHDK +I  +L +WPE  +KA+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GVPP   LPVLLDVGTDN+ LL+DPLY+G++HKR+RGK Y D +D FMQAV +KYG  CLIQFEDFAN NAFRIL+KYRN YCTFNDDIQGTASV + G+L A KI   KL D+ F+FQGAG AA GIA LL  A+ + G    EA  +I+M D  GL+VK R     E             F  +  H K L E V+ IKPT +IG A + G F   I+  + +F +RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF  V   D + F   Q NN+Y+FPG+AL +I   +R +++D+F+  AE ++  V++E LA+GR++P L TI++VS +IAV IA+YA+   IA+ +PEPKDK  +++ + Y+P Y     + Y +P +  K +D+
Sbjct:   57 TKKRGYDITRNPHLNKGMAFTLEERLQLGIHGLLPPCFLSQDVQVLRVMKSYETRINPLDKYILLMTLQDRNEKLFYRLLTSDIEEFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDKGHIATMLNSWPEDDIKAIVVTDGERILGLGDLGGYGMGIPVGKLALYTACGGVPPQQCLPVLLDVGTDNETLLNDPLYIGLKHKRVRGKEYDDLIDEFMQAVTDKYGMNCLIQFEDFANSNAFRILNKYRNRYCTFNDDIQGTASVAVAGVLAALKITKNKLSDHKFVFQGAGEAALGIAHLLIMAMAKEGVPHAEAAKRIWMVDSKGLIVKGRGHINHEKEE----------FAHEHPHIKTLEEVVETIKPTAIIGVAAIGGAFTEKIIKNMASFNERPIIFALSNPTSKAECTAEQCYKLTEGRGIFASGSPFQKVTLADGRTFYPGQGNNAYVFPGVALGVITCGVRHISDDIFLTTAEAISDMVTEEHLAEGRLYPPLSTIREVSFKIAVKIADYAYKQEIASWYPEPKDKEAFVLSNVYSPDYDSFTLDTYTWPKDAMKVQDV 615

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BLAST of Malic enzyme vs. Ensembl Cavefish
Match: me1 (NADP-dependent malic enzyme [Source:NCBI gene;Acc:103030051])

HSP 1 Score: 601.668 bits (1550), Expect = 0.000e+0
Identity = 286/544 (52.57%), Postives = 384/544 (70.59%), Query Frame = 2

Query:  227 AFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*E 1855
            AF+L+ER  LGIHGLLPP   + + Q LR + N     DDL+R++FL+ LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ YGL FRRPRGL+ITIHD+ +I  +L++WPE+ ++A+VV+DGERILGLGDLG YGMGIPVGKL+LYTA  GVPP + LPV+LDVGTDN++LL DPLY+G+RH+R+RG  Y + ++ FM+AV ++YG  CLIQFEDFANVNAFR+L+KYRN Y TFNDDIQGTASV + GLL A ++   K+ D+  +FQGAG AA GIA L+  A+ + G +  E + KI+M D  GL+VK RD            + +   F       + L + V+E+KPT +IG A V G F   I+  +  F  RPI+FALSNPTSKAECTA + YT T+G+ I+A GSPF PV   D + F   Q NN+Y+FPG+ L +    +R ++ED+F+  AET+A+ V+++DLA+GR++P L +I+ VS +IAV I EYA+   IA H+PEP DK  ++    +N  Y     + Y +P E
Sbjct:    2 AFSLEERLQLGIHGLLPPAFLSQDVQLLRVLRNYELKKDDLDRYVFLMGLQDRNEKLFYRVLTSDIERFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDRHHISTLLQSWPEKDIRAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVPPQHCLPVMLDVGTDNEELLKDPLYIGLRHRRVRGSAYDELVEEFMKAVTDRYGMDCLIQFEDFANVNAFRLLTKYRNKYLTFNDDIQGTASVAVAGLLAALRVTKSKMSDHTIVFQGAGEAAMGIAELIAMAMEKEGLSHAECLKKIWMVDSKGLIVKGRD----------HLTAEKERFAHQHSQMRKLEDVVRELKPTAIIGVAAVAGAFTERIIRDMAEFNQRPIIFALSNPTSKAECTAEQCYTLTEGRGIFASGSPFDPVTLADGRRFYPGQGNNAYVFPGVGLGVTACAMRNISEDIFLITAETIAQMVTEKDLAEGRLYPPLPSIQHVSFKIAVKIVEYAYQHKIATHYPEPADKEAFVRSLIFNTNYDNFAVDSYGWPDE 535

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BLAST of Malic enzyme vs. Ensembl Cavefish
Match: me2 (malic enzyme 2, NAD(+)-dependent, mitochondrial [Source:NCBI gene;Acc:445233])

HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0
Identity = 266/563 (47.25%), Postives = 378/563 (67.14%), Query Frame = 2

Query:  164 FSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +++ K++G  ++ +P  NKG AF LKERQ+LG+ GLLPP + T + QA+R   NLRKI D L ++++L+ +Q+RNE LFY+V+ +++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +++ IL+NWPE  VKA+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P + LPV +DVGTDNQ LL DP Y+G+  +R R + Y D +D FM+AVV+KYG++ LIQFEDF N NAFR L KYR+ YCTFNDDIQGTASV + GLL A ++  + + D+  LF GAG               +   + +EA                           V  SP   I        K   +AV  +KPT +IG +G   +F  +++  + +  +RPI+FALSNPT+KAECTA +AYT T G+C++A GSPF PV  +D ++    Q NN+YIFPG+ALA+I+  +R +++ +F+ AA+TLA Q++DE+L+QGR++P L  I++VS+++A+ + EY +  G+A  +PEP DK  Y+    +N  Y   LP+ Y +P
Sbjct:   18 WAHTKEKGKPLMLNPHTNKGMAFTLKERQMLGLQGLLPPKIETQDIQAMRFQRNLRKISDPLQKYIYLMGIQERNERLFYRVLMEDIEALMPIVYTPTVGLACTQYGHIFRRPKGLFISIMDRGHVRSILDNWPETNVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPESCLPVCIDVGTDNQTLLRDPFYMGLYRRRERSQAYDDLIDEFMEAVVQKYGQETLIQFEDFGNHNAFRFLKKYRHKYCTFNDDIQGTASVALAGLLAARRVVGKPITDHTVLFLGAGEDRE-----------QPTDSNQEAF--------------------------VHPSPGFGI--------KTFLDAVNILKPTAIIGVSGAGRLFTHDVIKTMGSLNERPIIFALSNPTAKAECTAEDAYTLTQGRCLFASGSPFGPVSLEDGQILTPGQGNNAYIFPGVALAVILSGVRHISDTVFLEAAKTLAGQLTDEELSQGRLYPPLSNIREVSVQMAIKVMEYLYMKGMAFQYPEPVDKEAYVRSTVWNTNYDSFLPDVYDWP 535

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BLAST of Malic enzyme vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003810.1 (pep scaffold:Pmarinus_7.0:GL476420:967784:1019810:1 gene:ENSPMAG00000003478.1 transcript:ENSPMAT00000003810.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 489.189 bits (1258), Expect = 3.302e-169
Identity = 232/409 (56.72%), Postives = 297/409 (72.62%), Query Frame = 2

Query:  227 AFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGK 1453
            AF L+ERQ LGIHGLLPP   + + Q L  + N  +  +DL+R++ L+ LQDRNE LFYKV+  NVE  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHDK +I  +L++WPE  ++A+V++DGERILGLGDLG+YGMGIPVGKL+LYT   GV P   LPV+LDVGTDNQ LL+DP Y+G++ KR+RG  Y +F+D FMQA+V KYG+ CLIQFEDFAN NAFR+L KYR+ YCTFNDDIQGTA+V + G+L A ++   +L D+ FLFQGAG AA GIA LL   +   G   ++A+S+I+M D  GL++K RD            + +   F +D      L+EAV  ++PTVLIG A + GVF   IL  + +F +RPIV+ALSNPTSKAECTA + Y  T GK
Sbjct:    2 AFGLEERQHLGIHGLLPPCFLSQDVQVLHVIKNFERQTNDLDRYILLMGLQDRNERLFYKVLMSNVERFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDKGHIATVLKSWPESDIRAVVMTDGERILGLGDLGSYGMGIPVGKLALYTVCGGVQPQQCLPVMLDVGTDNQSLLEDPFYIGLKQKRVRGAAYDEFIDEFMQAIVNKYGKNCLIQFEDFANTNAFRLLRKYRDHYCTFNDDIQGTAAVALAGILAALRVTKNRLSDHKFLFQGAGEAAMGIAGLLVMGMEVEGTPRQKALSRIWMLDSKGLIIKSRD----------NLTLEKKEFAQDHPAVTTLSEAVHTLRPTVLIGVAAIAGVFTEAILRDMASFNERPIVYALSNPTSKAECTAEQCYKATQGK 400

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BLAST of Malic enzyme vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001935.1 (pep scaffold:Pmarinus_7.0:GL490498:1324:3654:1 gene:ENSPMAG00000001760.1 transcript:ENSPMAT00000001935.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.872 bits (320), Expect = 7.941e-35
Identity = 63/137 (45.99%), Postives = 90/137 (65.69%), Query Frame = 2

Query: 1442 TDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            T G+ I+A GSPF  V   D +VF   Q NN+YIFPG AL  I   +R + + +F+ +A+ LA QV+D+ LA+GR+FP L TI+DVS  IA+ + E A+  G+A+  PEP DKR  I  H+++P Y+  LP+ Y +P
Sbjct:    1 TQGQAIFASGSPFGEVKLADGRVFHPGQGNNAYIFPGAALGTIACGLRHIPDQIFLCSAQALAGQVTDKHLAEGRLFPPLNTIRDVSFNIALKVVEEAYRIGLASVLPEPVDKRALIQSHFFSPEYISFLPDIYTWP 137

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BLAST of Malic enzyme vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009618.1 (pep scaffold:Pmarinus_7.0:GL487013:196:1118:1 gene:ENSPMAG00000008692.1 transcript:ENSPMAT00000009618.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.79 bits (312), Expect = 4.148e-34
Identity = 61/113 (53.98%), Postives = 76/113 (67.26%), Query Frame = 2

Query:  836 VVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHR 1174
            V  +YG   LIQFEDF N NAFR++ KYR  YCTFNDDIQGTA+V + G+L A K+    L  + FLF GAG AATGIA L+  AL E G    +A  +++M+D  GL+VK R
Sbjct:    1 VPRRYGYNTLIQFEDFGNHNAFRLMRKYREKYCTFNDDIQGTAAVALAGMLAAQKVTGTTLSQSRFLFLGAGEAATGIANLIVMALTEQGVPPVDAYGQVWMYDKHGLMVKVR 113

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BLAST of Malic enzyme vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002089.1 (pep scaffold:Pmarinus_7.0:GL488934:952:2243:1 gene:ENSPMAG00000001897.1 transcript:ENSPMAT00000002089.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 122.094 bits (305), Expect = 9.718e-33
Identity = 55/135 (40.74%), Postives = 92/135 (68.15%), Query Frame = 2

Query: 1448 GKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            G+ ++A GSPFA V     ++    Q NN+Y+FPG+AL +I  ++R +T++ F+  AE +A +V+D  LA+GR++P L +I+D+S +IAV I E A+++G+A+ +PEP+DK   +  H Y+P Y   +P+ Y +P
Sbjct:    2 GRGVFASGSPFAAVSLPSGRMLHPGQGNNAYVFPGLALGVIAARVRHITDEAFLATAEMIAGEVTDAHLAEGRLYPPLSSIRDISFKIAVKIVEQAYASGMASLYPEPQDKEALVRSHVYSPDYDSFVPDCYTWP 136

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BLAST of Malic enzyme vs. Ensembl Yeast
Match: MAE1 (Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids [Source:SGD;Acc:S000001512])

HSP 1 Score: 317.39 bits (812), Expect = 2.305e-99
Identity = 195/567 (34.39%), Postives = 299/567 (52.73%), Query Frame = 2

Query:  194 VLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIK-RILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMK-----HHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAK--QVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPE-------------PKDKRE---YIMKHWYNPTYLP 1822
            +L  P  NKG+AF  +ER+   +  LLPP VNT++EQ  R    L  +   L ++ F+ SL+ +N+ L++ ++  +++  +PIIYTPT G A   Y   FR+P G+++ I +   I+ R+     ++ V  +VVSD E ILG+GD G  G+ I + KL+L T   G+ P   LPV LDVGT+N+ L  D LY+G +  RIRGK Y DF++ F++AV + Y    ++ FEDF   NA R+L KYR +  +FNDDIQGT +V +  L+ A K  NR LKD   L  GAGSA  GIA  +   ++  G  +EEA  KI++ D  GL+++  + +          +P   ++ K        + + L + V+ +KPT L+G +   G F  +++  +     RPI+F LSNPT   E    +    T+   + A GSPF PV      +  S+ NN Y FPGI L  ++ +   +T+ +   A + LA+   + + D   G + P L TI + S  +A ++   A   G A    E             P+D  E   ++    + P Y P
Sbjct:  103 LLNSPLFNKGSAFTQEEREAFNLEALLPPQVNTLDEQLERSYKQLCYLKTPLAKNDFMTSLRVQNKVLYFALIRRHIKELVPIIYTPTEGDAIAAYSHRFRKPEGVFLDITEPDSIECRLATYGGDKDVDYIVVSDSEGILGIGDQGIGGVRIAISKLALMTLCGGIHPGRVLPVCLDVGTNNKKLARDELYMGNKFSRIRGKQYDDFLEKFIKAVKKVYP-SAVLHFEDFGVKNARRLLEKYRYELPSFNDDIQGTGAVVMASLIAALKHTNRDLKDTRVLIYGAGSAGLGIADQIVNHMVTHGVDKEEARKKIFLMDRRGLILQSYEAN---------STPAQHVYAKSDAEWAGINTRSLHDVVENVKPTCLVGCSTQAGAFTQDVVEEMHKHNPRPIIFPLSNPTRLHEAVPADLMKWTNNNALVATGSPFPPV----DGYRISENNNCYSFPGIGLGAVLSRATTITDKMISAAVDQLAELSPLREGDSRPG-LLPGLDTITNTSARLATAVILQALEEGTARIEQEQVPGGAPGETVKVPRDFDECLQWVKAQMWEPVYRP 654

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BLAST of Malic enzyme vs. Ensembl Nematostella
Match: EDO33525 (Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A7SRZ0])

HSP 1 Score: 578.941 bits (1491), Expect = 0.000e+0
Identity = 289/558 (51.79%), Postives = 392/558 (70.25%), Query Frame = 2

Query:  182 RGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQV-SDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            RG D++RD  +NKG AF L+ERQ+LGIHGLLPP V + E QA R    L++  +DL +++ L++L +RNE LF++V+ D  E  +PI+YTPTVGLAC+KYG++FRRPRGL+I+IHDK +I+ I+ NWP   VKA+V++DGERILGLGDLG  GMGIPVGKL+LYT   G+ P   LPV++DVGT+N++LLDDP Y+GVR KR   + Y + +D F+QA ++++G   LIQFEDF N NAFR L KYRN  CTFNDDIQGTA+V + G+L + KI   +L D   +FQGAG AA GIA LL  A+ + G TEE+A  KI++ D  GL+VK RD        G+T   Q   F K+ ++   L + VK IKPT ++G A VPG F   I   + +F DRP++FALSNPTSKAECTA+  YT T+G+ I+A GSPF PV   D + FI  Q NN+YIFPG+AL +I  +   VT+D+F+ AAE +  Q+ +D  L +G ++P L  I++VS+++A+S+ + A+S+GIA   PEP+DK E I +H Y P Y+  +P  Y +P
Sbjct:   14 RGTDIMRDSHLNKGLAFTLEERQILGIHGLLPPCVISQEIQAQRVYRELQRKPNDLEKYIQLMALLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFISIHDKGHIRDIVSNWPTTEVKAIVMTDGERILGLGDLGCCGMGIPVGKLALYTVCGGIDPEGCLPVMIDVGTNNEELLDDPFYIGVRQKRCNTEDYDELIDEFIQAALQRFGPTTLIQFEDFGNHNAFRFLEKYRNKICTFNDDIQGTAAVAVAGVLASLKITKTRLSDQRIVFQGAGEAAIGIANLLVLAMKKEGLTEEQAKKKIWLVDSRGLVVKERDC------GGLT--EQKLAFAKEHEYIDNLTDVVKHIKPTTIVGVAAVPGAFTEEICRDMASFNDRPVIFALSNPTSKAECTALNCYTWTEGRGIFASGSPFDPVTLPDGRHFIPGQGNNAYIFPGLALGVIACEAAHVTDDMFLHAAEIMPPQILTDSQLEKGCLYPPLGDIREVSIKVALSVVKDAYSSGIACDMPEPEDKEELIRRHVYTPDYMSYMPETYSWP 563

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BLAST of Malic enzyme vs. Ensembl Nematostella
Match: EDO28487 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6B8])

HSP 1 Score: 276.944 bits (707), Expect = 4.630e-87
Identity = 126/222 (56.76%), Postives = 172/222 (77.48%), Query Frame = 2

Query:  182 RGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEK 847
            RG D++RD  +NKG AF L+ERQ+LGIHGLLPP V + E QA R    L++  +DL +++ L++L +RNE LF++V+ D  E  +PI+YTPTVGLAC+KYG++FRRPRGL+I+IHDK +I+ I+ NWP   VKA+V++DGERILGLGDLG  GMGIPVGKL+LYT   G+ P   LPV++DVGT+N++LLDDP Y+GVR KR   + Y + +D F+QA +++
Sbjct:   14 RGTDIMRDSHLNKGLAFTLEERQILGIHGLLPPCVISQEIQAQRVYRELQRKPNDLEKYIQLMALLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFISIHDKGHIRDIVSNWPTTEVKAIVMTDGERILGLGDLGCCGMGIPVGKLALYTVCGGIDPEGCLPVMIDVGTNNEELLDDPFYIGVRQKRCNTEDYDELIDEFIQAALQR 235

HSP 2 Score: 114.39 bits (285), Expect = 1.411e-27
Identity = 62/132 (46.97%), Postives = 81/132 (61.36%), Query Frame = 2

Query: 1052 SAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTD 1447
             AA GIA LL  A+ + G TEE+A  KI++ D  GL+VK RD        G+T   Q   F K+ ++   L + VK IKPT ++G A VPG F   I   + +F DRP++FALSNPTSKAECTA+  YT T+
Sbjct:  242 KAAIGIANLLVLAMKKEGLTEEQAKKKIWLVDSRGLVVKDRDC------GGLT--EQKLAFAKEHEYIDNLTDVVKHIKPTTIVGVAAVPGAFTEEICRDMASFNDRPVIFALSNPTSKAECTALNCYTWTE 365

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BLAST of Malic enzyme vs. Ensembl Medaka
Match: me3 (NADP-dependent malic enzyme [Source:NCBI gene;Acc:101155546])

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 309/570 (54.21%), Postives = 402/570 (70.53%), Query Frame = 2

Query:  170 YVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP*ELWKFKDI 1876
            Y K+RG D+ R+P +NKG AF L+ER  LGIHGLLPP   + + Q LR M +     + L++++ L++LQDRNE LFY+V+T ++E  +PI+YTPTVGLACQ+YGL FRRPRGL+ITIHD+ +I  +L +WP Q ++A+VV+DGERILGLGDLG+YGMGIPVGKL+LYTA  GV P   LPVLLDVGTDNQ LLDDPLY+G++HKRIRGK Y + +D FMQAV +KYG  CLIQFEDFAN NAFRIL+KYRN YCTFNDDIQGTASV + G+L A KI   KL ++ F+FQGAG AA GIA LL  A+ + G + EEA  +I+M D  GL+VK R     E             F  D  H K L E V+ IKPT +IG A V G F   I+  +  F  RPI+FALSNPTSKAECTA + Y  T+G+ I+A GSPF+ V   D + F   Q NN+Y+FPG+AL I+   +R +++D+F+  AE +A  V++E LA+GR++P L TI++VS +IAV I  +A+   IA+ +PEPKDK  +++ H Y+P Y     + Y +P E  K +D+
Sbjct:   71 YTKKRGYDITRNPYLNKGMAFTLEERLQLGIHGLLPPCFLSQDVQVLRVMKSYEPRTNPLDKYILLMTLQDRNEKLFYRVLTSDIEEFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHIATMLNSWPVQNIRAIVVTDGERILGLGDLGSYGMGIPVGKLALYTACGGVQPQQCLPVLLDVGTDNQALLDDPLYIGLKHKRIRGKEYDELIDEFMQAVTDKYGMNCLIQFEDFANSNAFRILNKYRNRYCTFNDDIQGTASVAVAGILAALKITKNKLSEHTFVFQGAGEAALGIAHLLIMAMAKEGVSPEEAAKRIWMVDSKGLIVKGRSHVNHEKAE----------FAHDHPHLKTLEEVVQTIKPTAIIGVAAVGGAFTEKIITDMAAFNQRPIIFALSNPTSKAECTAEQCYKLTEGRGIFASGSPFSKVTLADGRTFYPGQGNNAYVFPGVALGIMACGVRHISDDIFLTTAEAIADMVTEEHLAEGRLYPPLSTIREVSFKIAVKIVNHAYKHNIASFYPEPKDKEAFVLSHVYSPDYDSFTVDTYSWPQEAMKVQDV 630

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BLAST of Malic enzyme vs. Ensembl Medaka
Match: me1 (malic enzyme 1 [Source:NCBI gene;Acc:101159698])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 305/565 (53.98%), Postives = 405/565 (71.68%), Query Frame = 2

Query:  158 RSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            ++F Y K+RG DV R+P +NKG AF+++ER  LGIHGLLPP   + + Q LR + N     DDL+R++FL+ LQDRNE LFY++++ +VE  +PIIYTPTVGLACQ+YGL+FRRPRGL+ITIHD  +I  IL+NWPE+ +KA+ V+DGERILGLGDLG +GMGIPVGKL+LYTA  G+PP   LPV+LDVGTDN+ LL DPLYLG+RHKR+RG+ Y D +D FM+AV ++YG  CLIQFEDFANVNAFR+LSKYRN YCTFNDDIQGTA+V + GLL A +I   K+ D+  +FQGAG AA GIA L+  ++ + G ++EE++ KI+M D  GL+VK RD            + +   F       K L + V+E++PT +IG A VPG F+  I+  + +F +RPI+FALSNPTSKAECTA + YT T+G+ I+A GSPF PV   D K     Q NN+YIFPG+ L++    IR +TE++F+ AAE LA  V++ DLA+GR++P L +I+DVSL++A  I EYA+   IA+  PEP DK  ++    +N  Y     + Y +P
Sbjct:   23 KTFVYTKKRGYDVTRNPNLNKGMAFSMEERLQLGIHGLLPPCFISQDVQLLRVLKNYDMKKDDLDRYVFLMGLQDRNEKLFYRLISSHVERFMPIIYTPTVGLACQQYGLIFRRPRGLFITIHDSGHIASILQNWPEKDIKAVCVTDGERILGLGDLGCHGMGIPVGKLALYTACGGMPPQQCLPVMLDVGTDNEALLKDPLYLGLRHKRVRGQPYDDLLDEFMKAVSDRYGMDCLIQFEDFANVNAFRLLSKYRNKYCTFNDDIQGTAAVAVAGLLAALRITKSKMCDHTIVFQGAGEAAMGIAELITMSMEKEGLSKEESLKKIWMVDSKGLIVKGRD----------HLTHEKERFAHSHSQMKNLEDVVRELRPTAIIGVAAVPGAFSEQIIKDMASFNERPIIFALSNPTSKAECTAEQCYTLTEGRAIFASGSPFDPVTLPDGKTLYPGQGNNAYIFPGVGLSVTACAIRHITEEIFLTAAEALAHMVTERDLAEGRLYPPLSSIRDVSLKLAAKIIEYAYKHNIASLRPEPSDKDAFVRSLCFNTAYDDFTVDTYHWP 577

[Close]

BLAST of Malic enzyme vs. Ensembl Medaka
Match: me2 (malic enzyme 2 [Source:NCBI gene;Acc:101168546])

HSP 1 Score: 602.823 bits (1553), Expect = 0.000e+0
Identity = 293/565 (51.86%), Postives = 404/565 (71.50%), Query Frame = 2

Query:  164 FSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKH--HKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +++ K++G  ++ +P  NKG AF L+ERQ+LGIHGLLPP V T + Q LR   NL+K+ D L ++++L+ +Q+RNE LFY+V+ +++E  +PI+YTPTVGLAC +YG +FRRP+GL+I+I D+ +++ IL+NWPE  V A+VV+DGERILGLGDLG YGMGIPVGKL LYTA AG+ P   LPV++DVGT+N+ LL DP Y+G+  KR R + Y D +D FM+AVV KYG+  LIQFEDF N NAFR L KYR  YCTFNDDIQGTASV + GLL A +   + +  +  LF GAG AA GIA L+  A++E+G T+ EA  KI+M+D  GLLVK R    Q+T +          FV D     H  L +AV  IKPT +IG AG   +F  +++ ++ TF +RPI+FALSNPT+KAECTA EAYT TDG+C++A GSPF PV   + +VF   Q NN+YIFPG+ALA+I+  +R +++ +F+ AA+ LA+Q++DE+LA+GR++P L  I++VS++IA+   EY +S G+A  +PEP DK  +I    +N +Y   LP+ Y +P
Sbjct:   20 WAHTKEKGKPLMLNPHTNKGMAFTLQERQILGIHGLLPPKVETQDIQTLRFQKNLKKMTDPLEKYIYLMGIQERNERLFYRVLMEDIEELMPIVYTPTVGLACTQYGHIFRRPKGLFISILDRGHVRSILDNWPETNVSAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPEKCLPVVIDVGTNNETLLKDPFYMGLYQKRDRSQAYDDLIDEFMEAVVNKYGQDTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTASVALAGLLAAQRAIGKPITQHKLLFLGAGEAALGIANLIVMAMMEAGMTQAEAQEKIWMYDAHGLLVKGRS---QKTDN------NQEAFVHDSPGDVHSFL-DAVNTIKPTAIIGVAGAGRLFTHDVIKVMGTFNERPIIFALSNPTNKAECTAEEAYTLTDGRCLFASGSPFGPVTLSNGQVFTPGQGNNAYIFPGVALAVILSGVRHISDTVFLEAAKALAEQLTDEELAEGRLYPPLSNIREVSVQIAIKAMEYVYSKGMAFRYPEPMDKNGFIRATVWNTSYDSFLPDTYDWP 574

[Close]

BLAST of Malic enzyme vs. Planmine SMEST
Match: SMESG000045895.1 (SMESG000045895.1)

HSP 1 Score: 1189.48 bits (3076), Expect = 0.000e+0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 2

Query:  131 MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP
Sbjct:    1 MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 573

[Close]

BLAST of Malic enzyme vs. Planmine SMEST
Match: SMESG000045895.1 (SMESG000045895.1)

HSP 1 Score: 1163.29 bits (3008), Expect = 0.000e+0
Identity = 565/573 (98.60%), Postives = 565/573 (98.60%), Query Frame = 2

Query:  131 MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVK        TRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP
Sbjct:    1 MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVK--------TRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 565

[Close]

BLAST of Malic enzyme vs. Planmine SMEST
Match: SMESG000026160.1 (SMESG000026160.1)

HSP 1 Score: 762.296 bits (1967), Expect = 0.000e+0
Identity = 357/561 (63.64%), Postives = 448/561 (79.86%), Query Frame = 2

Query:  167 SYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +Y+K RG+DVLR P  NK TAF +KER LLGIHGLLPP V+T+ EQ++R MI+L+K  DDL +HLFL SL +RNE LFYKV+TDN+EYC+P+IYTPTVGLACQKYGLMFRR RGLYITIHDKM+I++IL NWP  I+KA+VV+DGERILGLGDLGAYGMGIP+GKLSLYTALAGVPP  TLP+LLDVGT+N++LLDDP+YLG+RH+RIRG  Y +F+D FM  V+EKYGR  LIQFEDF NVNA+RIL+KYR+ YCTFNDDIQGTASV +G LLGA KI+ RKL+DN FLFQGAGSAATGIA L+ +AL+  G +E+EAIS+IYMFD+DGLL K        +R   TF+ +N  + KD+   K L EAV  IKPT +IG AGV  VF + IL  +    D P +FALSNPTSK+EC+A EAY +T+GKC++A GSPF PV      +  +QCNNSYIFPGIALAII+F+IRP+T ++F+ AAETLA  +S+E++  GR+FP+L +IK+VS+ IA+++AEYA+   ++N  PEP +K ++I K+ ++P Y  SLP+ Y YP
Sbjct:   21 TYIKVRGVDVLRAPEFNKDTAFTIKERHLLGIHGLLPPAVDTLSEQSVRTMISLKKSGDDLQKHLFLTSLMERNEVLFYKVITDNIEYCMPLIYTPTVGLACQKYGLMFRRSRGLYITIHDKMHIRQILNNWPSDIIKAIVVTDGERILGLGDLGAYGMGIPIGKLSLYTALAGVPPRYTLPILLDVGTNNEELLDDPMYLGLRHERIRGAAYDEFLDIFMANVIEKYGRNTLIQFEDFGNVNAYRILNKYRDKYCTFNDDIQGTASVCLGALLGAEKISKRKLQDNKFLFQGAGSAATGIAELISKALMNRGDSEDEAISRIYMFDIDGLLTK--------SRRKSTFTTENIKYAKDLSETKSLEEAVNLIKPTAIIGVAGVGQVFTAPILRKMKETVDVPYIFALSNPTSKSECSAEEAYKNTEGKCVFASGSPFDPVDLDQTRYYPTQCNNSYIFPGIALAIILFKIRPITIEMFVIAAETLASLISEEEIVNGRIFPSLDSIKNVSISIAIAVAEYAYKVQLSNRIPEPLNKEKFIKKYMFSPKYRKSLPDLYHYP 573

[Close]

BLAST of Malic enzyme vs. Planmine SMEST
Match: SMESG000034828.1 (SMESG000034828.1)

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 294/561 (52.41%), Postives = 399/561 (71.12%), Query Frame = 2

Query:  173 VKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRK-IDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKD-KVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTYLPSLPNPYKYP 1849
            +K  GI++LR+PRIN+GTAF L+ERQLLG+ GLLPP  +    Q +R + NL    ++DL+R  +L+SLQDRNE+LF+K V +NVEYC+P++YTPTVGLAC+ YGL+FRRPRGLYITIHDK +I  IL+NW E  VK +VV+DGERILGLGDLGAYGMGIPVGKL+LYTAL G+ P   LP++LDVGT+N+ L++DP Y+G+R  R  G  Y DF++ FM +VVEK+G   LIQFEDFAN NAF  L+KY++DYCTFNDDIQGTA+V +GGLL   ++   +L +  FLF GAG A+ GIA LL + ++E G +E EAISKI+M D  GLLVK+        RS          F  D+ H + L+E V+ IKPT LIGAA V  VF  +IL+M+  + +RPI+FALSNP  +AECTA EAY +T+GKC+++ GSPF  V   D + F  S  +N+Y+FPGI  A+    I+P+++D+F+ AA+TLA  +  +DL +G ++P +  I  +S  ++ +  EYA+   +A H+PEP+DK  ++ +  Y   Y  ++P PY +P
Sbjct:   22 LKHCGIEILRNPRINRGTAFTLRERQLLGLQGLLPPIESPQANQVVRILDNLNNSCENDLSRFNYLMSLQDRNENLFFKTVMENVEYCMPLVYTPTVGLACKNYGLVFRRPRGLYITIHDKDHISDILDNWMENDVKIIVVTDGERILGLGDLGAYGMGIPVGKLNLYTALGGIKPDKCLPIMLDVGTNNKQLIEDPNYIGLRKPRTVGSEYEDFINRFMTSVVEKFGPNVLIQFEDFANHNAFNFLNKYKDDYCTFNDDIQGTAAVALGGLLACMRLLENRLSNQRFLFAGAGEASVGIANLLIKLMMEDGISETEAISKIFMVDSKGLLVKN--------RSSEKLIGHKVKFAHDIPHIEGLSEIVENIKPTALIGAAAVGKVFTKDILHMMAKYNERPIIFALSNPVDRAECTAQEAYEYTEGKCLFSSGSPFGQVTLTDGRTFKPSTGHNAYLFPGIGFAVTTANIQPISDDIFVVAAKTLADFIELDDLEKGSLYPKVADIPKLSAVVSKNCIEYAYKNKMAAHYPEPEDKEAFLKRRRYLADYNEAVPFPYSWP 574

[Close]

BLAST of Malic enzyme vs. Planmine SMEST
Match: SMESG000033377.1 (SMESG000033377.1)

HSP 1 Score: 513.457 bits (1321), Expect = 5.006e-176
Identity = 253/544 (46.51%), Postives = 362/544 (66.54%), Query Frame = 2

Query:  185 GIDVLRDPRINKGTAFNLKERQLLGIHGLLPPGVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEYCLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVKALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVGTDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFEDFANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNIFLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDLDFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVFNSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPFAPVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAKQVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKREYIMKHWYNPTY 1816
            G  ++ +  +NKG  F   ER+ L + G+LP  V  +++Q    + NL++   +L ++++L+ L  +N  LF+ + + ++   LP+IYTPTVG AC K+G +F +P GLYI+I DK  I++IL  WPE  VKA+ V+DGERILGLGDLGA GMGIP+GKL+LYT + GV PS  LP+ LD GT+ + LL +  Y+G + +RI G+ Y +F+D FM+  ++ YG   LIQFEDF N  AF  L KYR  YC FNDDIQGTASV + GL+ A +I ++K+ +N FLF GAG A  GIA LL   + E G ++E A  +IY+ D  GL+V++R         GVT     +IF + M+  + L E VK++ PTVLIGA+G  G F + +L  +     +PI+ ALSNPTSK+ECTA EAYT T G  I++ GSPF PV Y ++V +  Q NN+Y+FPGIA A+I  +I+P+ + LF+ AA+ L+K+VS +DL  G++FP++  I DVSL +A+++ EYAF +  AN  PEP DK+ ++ + +YN  Y
Sbjct:    7 GYKIINNNFLNKGLGFTESERKALLLTGILPQLVLNIDQQEKLFLKNLKEKSSNLEKYIYLMDLHSKNIKLFFYIASRHISEILPLIYTPTVGTACLKFGEIFTKPCGLYISIKDKDNIEKILTMWPEANVKAICVTDGERILGLGDLGANGMGIPIGKLALYTMIGGVDPSYCLPITLDAGTNTKSLLQNEFYIGQKMERIIGEKYEEFVDIFMEICIKVYGADLLIQFEDFGNHYAFHFLQKYRMTYCMFNDDIQGTASVVVAGLINAIRIIDKKMSENKFLFFGAGEATLGIASLLVMIMQEDGLSDEGARERIYLVDSKGLVVENR------PSGGVTVF--KSIFARKMEPLESLLEIVKKVSPTVLIGASGQKGSFTAEVLKTMKDINSKPIIMALSNPTSKSECTAEEAYTSTKGAAIFSSGSPFNPVKYGNEVLLPGQANNAYVFPGIAFAVIHCKIKPIHDKLFLEAAKGLSKKVSSKDLEMGKIFPSMDQIADVSLGVALAVCEYAFRSKTANLRPEPVDKKLFLQQKFYNANY 542

The following BLAST results are available for this feature:

BLAST of Malic enzyme vs. Ensembl Human
BLAST of Malic enzyme vs. Ensembl Celegans
BLAST of Malic enzyme vs. Ensembl Fly
BLAST of Malic enzyme vs. Ensembl Zebrafish
BLAST of Malic enzyme vs. Ensembl Xenopus
BLAST of Malic enzyme vs. Ensembl Mouse
BLAST of Malic enzyme vs. UniProt/SwissProt
BLAST of Malic enzyme vs. TrEMBL
BLAST of Malic enzyme vs. Ensembl Cavefish
BLAST of Malic enzyme vs. Ensembl Sea Lamprey
BLAST of Malic enzyme vs. Ensembl Yeast
BLAST of Malic enzyme vs. Ensembl Nematostella
BLAST of Malic enzyme vs. Ensembl Medaka
BLAST of Malic enzyme vs. Planmine SMEST

BLAST of Malic enzyme vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ME1	0.000e+0	52.11	malic enzyme 1 [Source:HGNC Symbol;Acc:HGNC:6983]	[more]
ME3	0.000e+0	52.37	malic enzyme 3 [Source:HGNC Symbol;Acc:HGNC:6985]	[more]
ME3	0.000e+0	52.37	malic enzyme 3 [Source:HGNC Symbol;Acc:HGNC:6985]	[more]
ME3	0.000e+0	54.07	malic enzyme 3 [Source:HGNC Symbol;Acc:HGNC:6985]	[more]
ME2	0.000e+0	49.20	malic enzyme 2 [Source:HGNC Symbol;Acc:HGNC:6984]	[more]

BLAST of Malic enzyme vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3


Match Name	E-value	Identity	Description
men-1	0.000e+0	47.74	Malic enzyme [Source:UniProtKB/TrEMBL;Acc:Q9U296]	[more]
men-1	0.000e+0	47.74	Malic enzyme [Source:UniProtKB/TrEMBL;Acc:Q9U296]	[more]
men-1	0.000e+0	47.74	Malic enzyme [Source:UniProtKB/TrEMBL;Acc:Q9U296]	[more]

BLAST of Malic enzyme vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Men-b	0.000e+0	51.44	gene:FBgn0029155 transcript:FBtr0301708	[more]
Men-b	0.000e+0	51.44	gene:FBgn0029155 transcript:FBtr0301707	[more]
Men-b	0.000e+0	51.44	gene:FBgn0029155 transcript:FBtr0085110	[more]
Men	0.000e+0	52.08	gene:FBgn0002719 transcript:FBtr0082660	[more]
Men	0.000e+0	52.08	gene:FBgn0002719 transcript:FBtr0082661	[more]

BLAST of Malic enzyme vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
me3	0.000e+0	54.04	malic enzyme 3, NADP(+)-dependent, mitochondrial [...	[more]
me1	0.000e+0	52.04	malic enzyme 1, NADP(+)-dependent, cytosolic [Sour...	[more]
me1	0.000e+0	52.04	malic enzyme 1, NADP(+)-dependent, cytosolic [Sour...	[more]
me2	0.000e+0	51.15	malic enzyme 2, NAD(+)-dependent, mitochondrial [S...	[more]
me2	0.000e+0	51.15	malic enzyme 2, NAD(+)-dependent, mitochondrial [S...	[more]

BLAST of Malic enzyme vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
abhd4	0.000e+0	52.07	abhydrolase domain containing 4 [Source:Xenbase;Ac...	[more]
abhd4	0.000e+0	52.83	abhydrolase domain containing 4 [Source:Xenbase;Ac...	[more]
abhd4	0.000e+0	53.11	abhydrolase domain containing 4 [Source:Xenbase;Ac...	[more]
abhd4	0.000e+0	52.92	abhydrolase domain containing 4 [Source:Xenbase;Ac...	[more]
abhd4	0.000e+0	53.21	abhydrolase domain containing 4 [Source:Xenbase;Ac...	[more]

BLAST of Malic enzyme vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Me3	0.000e+0	52.55	malic enzyme 3, NADP(+)-dependent, mitochondrial [...	[more]
Me1	0.000e+0	51.41	malic enzyme 1, NADP(+)-dependent, cytosolic [Sour...	[more]
Me2	0.000e+0	49.38	malic enzyme 2, NAD(+)-dependent, mitochondrial [S...	[more]
Me1	0.000e+0	51.73	malic enzyme 1, NADP(+)-dependent, cytosolic [Sour...	[more]
Me3	1.334e-20	66.67	malic enzyme 3, NADP(+)-dependent, mitochondrial [...	[more]

BLAST of Malic enzyme vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P28227\|MAOX_ANAPL	0.000e+0	53.98	NADP-dependent malic enzyme OS=Anas platyrhynchos ...	[more]
sp\|P40927\|MAOX_COLLI	0.000e+0	53.45	NADP-dependent malic enzyme OS=Columba livia OX=89...	[more]
sp\|P48163\|MAOX_HUMAN	0.000e+0	52.11	NADP-dependent malic enzyme OS=Homo sapiens OX=960...	[more]
sp\|Q8BMF3\|MAON_MOUSE	0.000e+0	52.55	NADP-dependent malic enzyme, mitochondrial OS=Mus ...	[more]
sp\|Q16798\|MAON_HUMAN	0.000e+0	52.37	NADP-dependent malic enzyme, mitochondrial OS=Homo...	[more]

BLAST of Malic enzyme vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A3Q0KLC0	0.000e+0	58.70	Malic enzyme OS=Schistosoma mansoni OX=6183 PE=3 S...	[more]
A0A0R3SQ67	0.000e+0	58.08	Malic enzyme OS=Hymenolepis diminuta OX=6216 GN=HD...	[more]
A0A0V0J3Q3	0.000e+0	59.55	Malic enzyme OS=Schistocephalus solidus OX=70667 G...	[more]
A0A5K3F5P2	0.000e+0	57.89	Malic enzyme OS=Mesocestoides corti OX=53468 PE=4 ...	[more]
A0A183ME64	0.000e+0	58.48	Malic enzyme OS=Schistosoma margrebowiei OX=48269 ...	[more]

BLAST of Malic enzyme vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
me1	0.000e+0	52.20	NADP-dependent malic enzyme [Source:NCBI gene;Acc:...	[more]
me3	0.000e+0	53.60	NADP-dependent malic enzyme-like [Source:NCBI gene...	[more]
me1	0.000e+0	52.57	NADP-dependent malic enzyme [Source:NCBI gene;Acc:...	[more]
me2	0.000e+0	47.25	malic enzyme 2, NAD(+)-dependent, mitochondrial [S...	[more]

BLAST of Malic enzyme vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4


Match Name	E-value	Identity	Description
ENSPMAT00000003810.1	3.302e-169	56.72	pep scaffold:Pmarinus_7.0:GL476420:967784:1019810:...	[more]
ENSPMAT00000001935.1	7.941e-35	45.99	pep scaffold:Pmarinus_7.0:GL490498:1324:3654:1 gen...	[more]
ENSPMAT00000009618.1	4.148e-34	53.98	pep scaffold:Pmarinus_7.0:GL487013:196:1118:1 gene...	[more]
ENSPMAT00000002089.1	9.718e-33	40.74	pep scaffold:Pmarinus_7.0:GL488934:952:2243:1 gene...	[more]

BLAST of Malic enzyme vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
MAE1	2.305e-99	34.39	Mitochondrial malic enzyme; catalyzes the oxidativ...	[more]

BLAST of Malic enzyme vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO33525	0.000e+0	51.79	Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A7SRZ0]	[more]
EDO28487	4.630e-87	56.76	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Malic enzyme vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
me3	0.000e+0	54.21	NADP-dependent malic enzyme [Source:NCBI gene;Acc:...	[more]
me1	0.000e+0	53.98	malic enzyme 1 [Source:NCBI gene;Acc:101159698]	[more]
me2	0.000e+0	51.86	malic enzyme 2 [Source:NCBI gene;Acc:101168546]	[more]

BLAST of Malic enzyme vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000045895.1	0.000e+0	100.00	SMESG000045895.1	[more]
SMESG000045895.1	0.000e+0	98.60	SMESG000045895.1	[more]
SMESG000026160.1	0.000e+0	63.64	SMESG000026160.1	[more]
SMESG000034828.1	0.000e+0	52.41	SMESG000034828.1	[more]
SMESG000033377.1	5.006e-176	46.51	SMESG000033377.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005657 ID=SMED30005657|Name=Malic enzyme|organism=Schmidtea mediterranea sexual|type=transcript|length=2047bp
AAAACTTATTGCAGGAAATTTAAATGAAAATTATCTGTATCCCGACACTT
GTTTCAATCATAGATTTATGTGACCTTTATGGACACAAATATTTATTAAA
TTCAAGATCTTTCGTGAGAACTTAAAATAAATGGCATCTTTTAATAAATT
ATTTCAAAGGTCGTTCTCATATGTGAAACAAAGAGGAATAGATGTTCTTA
GAGACCCAAGAATTAATAAAGGCACAGCTTTTAATTTAAAAGAACGCCAG
TTGCTAGGTATTCATGGTTTATTGCCCCCAGGAGTTAATACAATGGAAGA
GCAAGCTTTACGCTGTATGATAAATTTAAGAAAAATTGATGACGATTTGA
ATCGTCACTTGTTTCTCATTTCCCTACAAGACAGAAATGAAGATTTATTT
TACAAAGTGGTTACAGATAACGTAGAATATTGTCTACCAATAATTTACAC
ACCCACAGTTGGTTTGGCTTGTCAGAAATACGGTCTAATGTTTCGACGCC
CAAGAGGTCTATATATCACGATTCATGACAAAATGTACATCAAAAGAATA
CTTGAAAACTGGCCAGAACAGATTGTTAAAGCTTTGGTGGTAAGTGATGG
TGAAAGGATTTTGGGACTGGGTGATCTAGGAGCCTATGGAATGGGTATAC
CCGTGGGAAAGTTGTCTTTATATACAGCACTTGCTGGTGTGCCTCCATCA
AATACACTTCCAGTGTTACTGGATGTCGGAACGGATAATCAAGATTTGTT
GGATGATCCCCTTTATTTGGGCGTAAGACACAAGCGAATACGTGGCAAAA
TGTACTTTGATTTTATGGATGCTTTTATGCAAGCTGTAGTGGAAAAATAC
GGTCGACAGTGTTTAATTCAATTTGAAGATTTTGCAAATGTAAACGCTTT
TAGAATTCTCAGTAAATATAGAAATGATTACTGTACATTCAACGATGACA
TACAAGGCACGGCATCTGTGACAATTGGTGGTTTGTTAGGAGCTTGTAAA
ATTGCGAATAGAAAGTTAAAAGACAATATATTTTTGTTTCAAGGAGCTGG
ATCCGCTGCTACAGGAATCGCTTTGTTACTAAAAAGGGCTCTTATTGAAA
GCGGAGCTACAGAAGAAGAAGCAATAAGCAAAATTTATATGTTTGATGTT
GACGGACTTCTTGTAAAACATCGAGATTTAGATTTTCAAGAGACAAGAAG
TGGAGTTACATTTTCTCCTCAAAACACTATCTTTGTCAAAGATATGAAAC
ATCACAAAATTTTAGCAGAAGCAGTTAAAGAAATAAAGCCGACTGTTTTG
ATTGGAGCCGCTGGTGTTCCTGGTGTTTTCAATTCAAATATTCTCAACAT
GCTAACCACATTTACTGATAGACCGATTGTTTTTGCATTAAGTAACCCAA
CATCGAAGGCTGAATGCACTGCTATTGAAGCTTATACTCACACTGATGGC
AAATGTATTTATGCTTGTGGAAGCCCATTTGCACCGGTACACTATAAGGA
CAAAGTTTTCATTGCAAGCCAGTGTAATAATTCTTACATATTTCCAGGAA
TAGCATTAGCTATAATTATGTTTCAAATCCGACCAGTAACAGAAGATTTA
TTTATAAGAGCAGCTGAGACATTAGCGAAGCAAGTTAGCGATGAAGATTT
AGCACAAGGACGAGTTTTTCCAGCTCTTTGGACTATAAAAGATGTATCAT
TGGAGATTGCGGTATCTATTGCGGAGTATGCATTTTCGGCGGGAATAGCT
AATCATCATCCTGAACCTAAAGATAAACGAGAATATATTATGAAACATTG
GTATAACCCAACTTATCTACCAAGCCTTCCAAATCCTTATAAATACCCAT
AAGAATTATGGAAATTTAAAGATATTCATCATATTTATCTTTTTATTTTA
TTTGTGAATTACAATTTATTACAACCTTTCGAAATATTTGTGAACGATTT
TCTGCAAAATAGGAATATTATATTTGAAATAATATTTTCAAGATGTTATT
TGTACTTCTATAATTTACCTTATATCCATTAAATAATACATTATGAC

protein sequence of SMED30005657-orf-1

>SMED30005657-orf-1 ID=SMED30005657-orf-1|Name=SMED30005657-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=574bp
MASFNKLFQRSFSYVKQRGIDVLRDPRINKGTAFNLKERQLLGIHGLLPP
GVNTMEEQALRCMINLRKIDDDLNRHLFLISLQDRNEDLFYKVVTDNVEY
CLPIIYTPTVGLACQKYGLMFRRPRGLYITIHDKMYIKRILENWPEQIVK
ALVVSDGERILGLGDLGAYGMGIPVGKLSLYTALAGVPPSNTLPVLLDVG
TDNQDLLDDPLYLGVRHKRIRGKMYFDFMDAFMQAVVEKYGRQCLIQFED
FANVNAFRILSKYRNDYCTFNDDIQGTASVTIGGLLGACKIANRKLKDNI
FLFQGAGSAATGIALLLKRALIESGATEEEAISKIYMFDVDGLLVKHRDL
DFQETRSGVTFSPQNTIFVKDMKHHKILAEAVKEIKPTVLIGAAGVPGVF
NSNILNMLTTFTDRPIVFALSNPTSKAECTAIEAYTHTDGKCIYACGSPF
APVHYKDKVFIASQCNNSYIFPGIALAIIMFQIRPVTEDLFIRAAETLAK
QVSDEDLAQGRVFPALWTIKDVSLEIAVSIAEYAFSAGIANHHPEPKDKR
EYIMKHWYNPTYLPSLPNPYKYP*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000101	muscle cell
PLANA:0002089	reproductive organ
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR037062	Malic_N_dom_sf
IPR036291	NAD(P)-bd_dom_sf
IPR001891	Malic_OxRdtase
IPR012302	Malic_NAD-bd
IPR012301	Malic_N_dom
IPR016040	NAD(P)-bd_dom
IPR015884	Malic_enzyme_CS

Vocabulary: **biological process**
Term	Definition
GO:0006108	malate metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **molecular function**
Term	Definition
GO:0004470	malic enzyme activity
GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0051287	NAD binding
GO:0016491	oxidoreductase activity
GO:0046872	metal ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 111..129 score: 65.79 coord: 51..73 score: 56.96 coord: 136..152 score: 70.88 coord: 167..183 score: 59.85 coord: 15..44 score: 71.75 coord: 278..294 score: 61.62
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 1..434 e-value: 1.5E-162 score: 539.8
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 1..130 e-value: 2.1E-50 score: 183.5
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 3..130 e-value: 4.0E-54 score: 183.2
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 140..401 e-value: 2.0E-80 score: 283.2
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 140..400 e-value: 3.4E-86 score: 288.7
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	G3DSA:3.40.50.10380		coord: 1..137 e-value: 6.4E-67 score: 227.3
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 138..439 e-value: 1.2E-103 score: 348.6
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 3..433
None	No IPR available	PANTHER	PTHR23406:SF60	MALIC ENZYME-RELATED	coord: 3..433
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 140..426 e-value: 8.50637E-122 score: 353.777
None	No IPR available	SUPERFAMILY	SSF53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 5..139
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 136..152
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 140..434