SMED30005647
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005647 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30005647 aligns in the following genomic locations:
Homology
BLAST of SMED30005647 vs. TrEMBL
Match: A0A4Z2DXE4 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_000735 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 4.171e-16 Identity = 65/250 (26.00%), Postives = 119/250 (47.60%), Query Frame = 3 Query: 39 SPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVS------------------SVAGDC-SNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLI 716 +P+ R+ +L S N T N +S +P+ KLR+MS F +D++ L+DP + F + +++ L +E+K+++ +EHL+ DYE SR + + RY L+ MIKRT+MH++++ + GD + K KE+ ++ E ++ T DE+ + + +++ E + RI ++I I +L+ Y+ KT + RY +K VK +I Sbjct: 20 TPVTRKSLEAISLSRKSLCNNVTINTSSNELISFSPSD------------------KLRRMSMLFSIDRVKTREAIMAALEDPARTEFKHLKIENEYLTSEWKQIRKILEHLQTDYETSRPLDPLRRYKNLQSMIKRTVMHIRITEPSSLKRLSIHPNNSGDIPLQGDVLQSETKTKEMQKRVGEICDNFTIDELTIQNRRLRDLNERLENRIQLIIQTINELDCLYHSVKTDGFFKRYTSLKVAVKRII 251
BLAST of SMED30005647 vs. TrEMBL
Match: A0A4V3SFK2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004115 PE=4 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 1.805e-15 Identity = 70/258 (27.13%), Postives = 126/258 (48.84%), Query Frame = 3 Query: 87 SCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPS--------------------KLRKMSQFFHVDKISET------LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVA---GDCSNA------------IKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVE 737 S T +K P RR S+ PA+ ++ + S N G S KLR+MS F +DK S+T +L+DP + + ++ ++ L E+K+L+ +VE L DYE +R + + RY +L+ M KRT+MHL+++ V GD A K +E+ ++ E+ T +E+ E + ++EN + + RI +++ I +L+ Y +K+ RY ++ VK+++ ++E Sbjct: 3 SLPTTSVQKFP--RRTSVIPAAASYTRPSYESSNKTGHSSIASASSDRNSIEGSGFNAGEKLRRMSTLFSIDK-SKTREAIMAVLEDPARTEYRKLKNTNEHLTAEWKQLRKFVEQLASDYESTRQLDPIRRYRQLQAMTKRTVMHLRLTEVGCLSGDPLKAEVPVQGSILQSEAKEREIEKRIMRMCENYTVEELGPENRRLRENNDRLETRIQLIVQTINELDVLYRSSKSDSFVKRYGALREAVKEIVTNKELIE 257
BLAST of SMED30005647 vs. TrEMBL
Match: A0A504YX25 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_10226 PE=4 SV=1) HSP 1 Score: 80.1073 bits (196), Expect = 1.931e-14 Identity = 50/158 (31.65%), Postives = 83/158 (52.53%), Query Frame = 3 Query: 84 SSCQNTPTKKVPQNRRVSLAPASGTF---------------IKDKRSSINSEG---TPS-KLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNA 485 +S N+ K P RR S+ PA+ F I + S N E +P+ KLR+MS F +DK L+DP + + ++ Q++ L TE+K+L+ ++E L+ DYE SR + + R+ +L+ M KRT+MHL+++ V ++A Sbjct: 2 ASGANSSATKFP--RRTSVTPAAAPFSRSMTDAGTSGRNTSISRRSMSQNPESGGFSPADKLRRMSMLFSIDKTRTRDAILAALEDPARTEYRRLKYQNEHLTTEWKQLRKFIEQLQTDYEASRQLDPIRRFRQLQAMTKRTVMHLRLTEVGSASADA 157
BLAST of SMED30005647 vs. TrEMBL
Match: A0A074ZIM9 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08901 PE=4 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 1.958e-14 Identity = 66/250 (26.40%), Postives = 123/250 (49.20%), Query Frame = 3 Query: 108 KKVPQNRRVSLAPASGTFIKDKRSSINSEGTPS--------------------KLRKMSQFFHVDKISET------LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVA---GDCSNA------------IKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIV 734 KK P RR S+ PA+ ++ + S N G S KLR+MS F +D+ S+T +L+DP + + ++ ++ L E+K+L+ +VE L DYE +R + + RY +L+ M KRT+MHL+++ V GD A K +E+ ++ E+ T +E+ E + ++EN + + RI +++ I +L+ Y +K+ RY ++ +K+++ ++ Sbjct: 23 KKFP--RRTSVTPAAASYTRPSNESSNKTGRLSLASASSDRNSIEASGFNAGEKLRRMSTLFSIDR-SKTREAIMAVLEDPARTEYRKLKNTNEHLTAEWKQLRKFVEQLASDYESTRQLDPIRRYRQLQAMTKRTVMHLRLTEVGCLSGDPLKAEVPVQGSILQSEAKEREIEKRIMRMCENYTVEELGPENRRLRENNDRLETRIQLIVQTINELDVLYRSSKSDSFVKRYGALREAIKEIVTNKELI 269
BLAST of SMED30005647 vs. TrEMBL
Match: A0A2H1CIT0 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_05927 PE=4 SV=1) HSP 1 Score: 80.4925 bits (197), Expect = 2.167e-14 Identity = 50/158 (31.65%), Postives = 83/158 (52.53%), Query Frame = 3 Query: 84 SSCQNTPTKKVPQNRRVSLAPASGTF---------------IKDKRSSINSEG---TPS-KLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNA 485 +S N+ K P RR S+ PA+ F I + S N E +P+ KLR+MS F +DK L+DP + + ++ Q++ L TE+K+L+ ++E L+ DYE SR + + R+ +L+ M KRT+MHL+++ V ++A Sbjct: 2 ASGANSSATKFP--RRTSVTPAAAPFSRSMTDAGTSGRNTSISRRSMSQNPESGGFSPADKLRRMSMLFSIDKTRTRDAILAALEDPARTEYRRLKYQNEHLTTEWKQLRKFIEQLQTDYEASRQLDPIRRFRQLQAMTKRTVMHLRLTEVGSASADA 157
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000069423.1 (SMESG000069423.1) HSP 1 Score: 462.225 bits (1188), Expect = 5.384e-167 Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 3 Query: 12 MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 743 MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII Sbjct: 1 MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 244
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000080507.1 (SMESG000080507.1) HSP 1 Score: 110.923 bits (276), Expect = 1.922e-29 Identity = 73/206 (35.44%), Postives = 126/206 (61.17%), Query Frame = 3 Query: 111 KVPQNRRVSLAPASGTFIKDKRSSINS-EGTPSKLRKMSQFFHVDKISETLLKDP-EKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGD-CSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIH 719 K Q RRVS+ + +SS N+ + P+K+RKMSQFF+++K S+++ D EKE F+Q++EQ D+L E +R S+++ LK YE + ++ Y K MI+ + HLK+S V G+ CSNA ++KE+ K+++ Y+ L++ E + K E+ +I +Q+I + AI LE+ Y + K + + +Y++MK ++K I+ Sbjct: 11 KASQARRVSMVSLT-------KSSYNTADELPNKIRKMSQFFNIEKFSDSIWNDDNEKEIFLQLFEQQDNLTLENRRFHSFIDDLKIKYENEKQRYSLNCYFNYKNMIQCAVNHLKISHVTGEICSNA-RLKEVVSKADKIYKKLSNQERLEYKNEMVIDINLLKQQISKFVYAIEQLEKDYENIKQFSFFRKYKLMKMIIKKCIN 208
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000023281.1 (SMESG000023281.1) HSP 1 Score: 105.145 bits (261), Expect = 5.162e-27 Identity = 71/180 (39.44%), Postives = 103/180 (57.22%), Query Frame = 3 Query: 210 LRKMSQFFHVDKISETLLKDPE-KEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYE-MSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 743 LR+MS+F H E+LL K+ FI++Y+ + EYKRLK +++ L DY + ++RY LK MIKR +MHLKV + K E+ RK EE+YE L S+ + +EK +K IE Q R IL +DL+ Y +K Y TRYQM+ A++KDL+ +P + E+I Sbjct: 63 LRRMSKFLH-----ESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAK--EINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELI 235
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000044339.1 (SMESG000044339.1) HSP 1 Score: 85.8853 bits (211), Expect = 4.655e-20 Identity = 61/174 (35.06%), Postives = 102/174 (58.62%), Query Frame = 3 Query: 258 LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCS----NAIKI----------------KELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIH 719 L+KDP +E + ++ E++D EYKRL+S+V+ L+ DY S+ + + RY LK MIKRT++HLK+ + S NA+ I E A+K EER ++L S+EI E ++++E+I++++++ ++ AI +LE Y +K YF RY +K ++K IH Sbjct: 24 LMKDPVREDYQRLAEEYDKSCEEYKRLRSFVDILEADYSSSKSLDVIRRYLSLKGMIKRTVLHLKLPHITSGKSDLNPNAVGISAMGNLLQGTRTFVKRSEQAKKWEERLQTLKSEEIIAENRKMEESIKDYKRKTAMITDAINNLEVEYTASKIYFPIRRYPALKQIIKRTIH 197
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000032554.1 (SMESG000032554.1) HSP 1 Score: 80.8777 bits (198), Expect = 2.651e-18 Identity = 48/169 (28.40%), Postives = 97/169 (57.40%), Query Frame = 3 Query: 258 LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLK-----------------VSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDL 713 +LKDP ++ F+++ ++D + + KR++ Y+E L+++Y + + RY +LK MIKR MHLK + ++A + +K E ARK EE+ + +TSDE+ +E + I+++++ + ++ + AI +E HY +++ + RYQ +K ++K++ Sbjct: 18 ILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNV 186 The following BLAST results are available for this feature:
BLAST of SMED30005647 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005647 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005647 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30005647 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005647 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005647 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005647 ID=SMED30005647|Name=SMED30005647|organism=Schmidtea mediterranea sexual|type=transcript|length=817bpback to top protein sequence of SMED30005647-orf-1 >SMED30005647-orf-1 ID=SMED30005647-orf-1|Name=SMED30005647-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=245bp MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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