SMED30005647

Overview
NameSMED30005647
Smed IDSMED30005647
Length (bp)817
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005647 (SMED30005647) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005647 (SMED30005647) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005647 (SMED30005647) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005647 (SMED30005647) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005647

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30005647SMESG000069423.1 SmedASXL_007543SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30005647SMESG000069423.1 dd_Smed_v4_21258_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005647SMESG000069423.1 dd_Smed_v4_21258_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005647SMESG000069423.1 dd_Smed_v4_21258_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005647SMESG000069423.1 dd_Smed_v4_21258_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30005647SMESG000069423.1 dd_Smed_v6_21258_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30005647SMESG000069423.1 Contig12068newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30005647SMESG000069423.1 Contig12068uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005647 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.013305:10533..11327 +2725
Homology
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BLAST of SMED30005647 vs. TrEMBL
Match: A0A4Z2DXE4 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_000735 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.171e-16
Identity = 65/250 (26.00%), Postives = 119/250 (47.60%), Query Frame = 3 
Query:   39 SPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVS------------------SVAGDC-SNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLI 716
            +P+ R+     +L   S  N  T     N  +S +P+                   KLR+MS  F +D++         L+DP +  F  +  +++ L +E+K+++  +EHL+ DYE SR  + + RY  L+ MIKRT+MH++++                   + GD   +  K KE+ ++  E  ++ T DE+  + + +++  E  + RI ++I  I +L+  Y+  KT   + RY  +K  VK +I
Sbjct:   20 TPVTRKSLEAISLSRKSLCNNVTINTSSNELISFSPSD------------------KLRRMSMLFSIDRVKTREAIMAALEDPARTEFKHLKIENEYLTSEWKQIRKILEHLQTDYETSRPLDPLRRYKNLQSMIKRTVMHIRITEPSSLKRLSIHPNNSGDIPLQGDVLQSETKTKEMQKRVGEICDNFTIDELTIQNRRLRDLNERLENRIQLIIQTINELDCLYHSVKTDGFFKRYTSLKVAVKRII 251
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BLAST of SMED30005647 vs. TrEMBL
Match: A0A4V3SFK2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004115 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.805e-15
Identity = 70/258 (27.13%), Postives = 126/258 (48.84%), Query Frame = 3 
Query:   87 SCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPS--------------------KLRKMSQFFHVDKISET------LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVA---GDCSNA------------IKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVE 737
            S   T  +K P  RR S+ PA+ ++ +    S N  G  S                    KLR+MS  F +DK S+T      +L+DP +  + ++   ++ L  E+K+L+ +VE L  DYE +R  + + RY +L+ M KRT+MHL+++ V    GD   A             K +E+ ++     E+ T +E+  E + ++EN +  + RI +++  I +L+  Y  +K+     RY  ++  VK+++    ++E
Sbjct:    3 SLPTTSVQKFP--RRTSVIPAAASYTRPSYESSNKTGHSSIASASSDRNSIEGSGFNAGEKLRRMSTLFSIDK-SKTREAIMAVLEDPARTEYRKLKNTNEHLTAEWKQLRKFVEQLASDYESTRQLDPIRRYRQLQAMTKRTVMHLRLTEVGCLSGDPLKAEVPVQGSILQSEAKEREIEKRIMRMCENYTVEELGPENRRLRENNDRLETRIQLIVQTINELDVLYRSSKSDSFVKRYGALREAVKEIVTNKELIE 257
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BLAST of SMED30005647 vs. TrEMBL
Match: A0A504YX25 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_10226 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.931e-14
Identity = 50/158 (31.65%), Postives = 83/158 (52.53%), Query Frame = 3 
Query:   84 SSCQNTPTKKVPQNRRVSLAPASGTF---------------IKDKRSSINSEG---TPS-KLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNA 485
            +S  N+   K P  RR S+ PA+  F               I  +  S N E    +P+ KLR+MS  F +DK          L+DP +  + ++  Q++ L TE+K+L+ ++E L+ DYE SR  + + R+ +L+ M KRT+MHL+++ V    ++A
Sbjct:    2 ASGANSSATKFP--RRTSVTPAAAPFSRSMTDAGTSGRNTSISRRSMSQNPESGGFSPADKLRRMSMLFSIDKTRTRDAILAALEDPARTEYRRLKYQNEHLTTEWKQLRKFIEQLQTDYEASRQLDPIRRFRQLQAMTKRTVMHLRLTEVGSASADA 157
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BLAST of SMED30005647 vs. TrEMBL
Match: A0A074ZIM9 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08901 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.958e-14
Identity = 66/250 (26.40%), Postives = 123/250 (49.20%), Query Frame = 3 
Query:  108 KKVPQNRRVSLAPASGTFIKDKRSSINSEGTPS--------------------KLRKMSQFFHVDKISET------LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVA---GDCSNA------------IKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIV 734
            KK P  RR S+ PA+ ++ +    S N  G  S                    KLR+MS  F +D+ S+T      +L+DP +  + ++   ++ L  E+K+L+ +VE L  DYE +R  + + RY +L+ M KRT+MHL+++ V    GD   A             K +E+ ++     E+ T +E+  E + ++EN +  + RI +++  I +L+  Y  +K+     RY  ++  +K+++    ++
Sbjct:   23 KKFP--RRTSVTPAAASYTRPSNESSNKTGRLSLASASSDRNSIEASGFNAGEKLRRMSTLFSIDR-SKTREAIMAVLEDPARTEYRKLKNTNEHLTAEWKQLRKFVEQLASDYESTRQLDPIRRYRQLQAMTKRTVMHLRLTEVGCLSGDPLKAEVPVQGSILQSEAKEREIEKRIMRMCENYTVEELGPENRRLRENNDRLETRIQLIVQTINELDVLYRSSKSDSFVKRYGALREAIKEIVTNKELI 269
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BLAST of SMED30005647 vs. TrEMBL
Match: A0A2H1CIT0 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_05927 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.167e-14
Identity = 50/158 (31.65%), Postives = 83/158 (52.53%), Query Frame = 3 
Query:   84 SSCQNTPTKKVPQNRRVSLAPASGTF---------------IKDKRSSINSEG---TPS-KLRKMSQFFHVDKISE-----TLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNA 485
            +S  N+   K P  RR S+ PA+  F               I  +  S N E    +P+ KLR+MS  F +DK          L+DP +  + ++  Q++ L TE+K+L+ ++E L+ DYE SR  + + R+ +L+ M KRT+MHL+++ V    ++A
Sbjct:    2 ASGANSSATKFP--RRTSVTPAAAPFSRSMTDAGTSGRNTSISRRSMSQNPESGGFSPADKLRRMSMLFSIDKTRTRDAILAALEDPARTEYRRLKYQNEHLTTEWKQLRKFIEQLQTDYEASRQLDPIRRFRQLQAMTKRTVMHLRLTEVGSASADA 157
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BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000069423.1 (SMESG000069423.1)

HSP 1 Score: 462.225 bits (1188), Expect = 5.384e-167
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 3 
Query:   12 MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 743
            MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII
Sbjct:    1 MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTFIKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 244
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BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000080507.1 (SMESG000080507.1)

HSP 1 Score: 110.923 bits (276), Expect = 1.922e-29
Identity = 73/206 (35.44%), Postives = 126/206 (61.17%), Query Frame = 3 
Query:  111 KVPQNRRVSLAPASGTFIKDKRSSINS-EGTPSKLRKMSQFFHVDKISETLLKDP-EKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGD-CSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIH 719
            K  Q RRVS+   +       +SS N+ +  P+K+RKMSQFF+++K S+++  D  EKE F+Q++EQ D+L  E +R  S+++ LK  YE  +   ++  Y   K MI+  + HLK+S V G+ CSNA ++KE+  K+++ Y+ L++ E  + K E+  +I   +Q+I   + AI  LE+ Y + K +  + +Y++MK ++K  I+
Sbjct:   11 KASQARRVSMVSLT-------KSSYNTADELPNKIRKMSQFFNIEKFSDSIWNDDNEKEIFLQLFEQQDNLTLENRRFHSFIDDLKIKYENEKQRYSLNCYFNYKNMIQCAVNHLKISHVTGEICSNA-RLKEVVSKADKIYKKLSNQERLEYKNEMVIDINLLKQQISKFVYAIEQLEKDYENIKQFSFFRKYKLMKMIIKKCIN 208
[Close]
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000023281.1 (SMESG000023281.1)

HSP 1 Score: 105.145 bits (261), Expect = 5.162e-27
Identity = 71/180 (39.44%), Postives = 103/180 (57.22%), Query Frame = 3 
Query:  210 LRKMSQFFHVDKISETLLKDPE-KEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYE-MSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII 743
            LR+MS+F H     E+LL     K+ FI++Y+  +    EYKRLK +++ L  DY  +      ++RY  LK MIKR +MHLKV       +   K  E+ RK EE+YE L S+ + +EK  +K  IE  Q R  IL    +DL+  Y  +K Y   TRYQM+ A++KDL+ +P + E+I
Sbjct:   63 LRRMSKFLH-----ESLLGGENVKQEFIKLYDFQELTVMEYKRLKQHIDTLAKDYSCLQTSNNWLSRYRMLKSMIKRCVMHLKVGEDTQISAGVAK--EINRKCEEKYEKLNSETLIEEKTMLKNQIEMHQARTAILREVFSDLDTQYTASKKYVALTRYQMLNALIKDLLARPNVKELI 235
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BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000044339.1 (SMESG000044339.1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.655e-20
Identity = 61/174 (35.06%), Postives = 102/174 (58.62%), Query Frame = 3 
Query:  258 LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSSVAGDCS----NAIKI----------------KELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIH 719
            L+KDP +E + ++ E++D    EYKRL+S+V+ L+ DY  S+  + + RY  LK MIKRT++HLK+  +    S    NA+ I                 E A+K EER ++L S+EI  E ++++E+I++++++  ++  AI +LE  Y  +K YF   RY  +K ++K  IH
Sbjct:   24 LMKDPVREDYQRLAEEYDKSCEEYKRLRSFVDILEADYSSSKSLDVIRRYLSLKGMIKRTVLHLKLPHITSGKSDLNPNAVGISAMGNLLQGTRTFVKRSEQAKKWEERLQTLKSEEIIAENRKMEESIKDYKRKTAMITDAINNLEVEYTASKIYFPIRRYPALKQIIKRTIH 197
[Close]
BLAST of SMED30005647 vs. Planmine SMEST
Match: SMESG000032554.1 (SMESG000032554.1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.651e-18
Identity = 48/169 (28.40%), Postives = 97/169 (57.40%), Query Frame = 3 
Query:  258 LLKDPEKEAFIQMYEQHDDLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLK-----------------VSSVAGDCSNAIKIKELARKSEERYESLTSDXXXXXXXXXXXXXXXXXXRIDILISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDL 713
            +LKDP ++ F+++  ++D +  + KR++ Y+E L+++Y   +  +   RY +LK MIKR  MHLK                 + ++A    + +K  E ARK EE+ + +TSDE+ +E + I+++++  + ++  +  AI  +E HY +++    + RYQ +K ++K++
Sbjct:   18 ILKDPTRDNFLRLSAEYDKVNDDLKRIQGYIETLEEEYNQCKMMDVFRRYQKLKGMIKRITMHLKMPGMTACAGAKPLGISAIGNLAQGTKDMVKRNEQARKREEQIKKMTSDELIRETKRIEDSLKNKKSQVAFISDAIRKMEIHYEESRNMNPFLRYQALKKIIKNV 186
The following BLAST results are available for this feature:
BLAST of SMED30005647 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2DXE44.171e-1626.00Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4V3SFK21.805e-1527.13Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A504YX251.931e-1431.65Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A074ZIM91.958e-1426.40Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A2H1CIT02.167e-1431.65Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
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BLAST of SMED30005647 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005647 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069423.15.384e-167100.00SMESG000069423.1[more]
SMESG000080507.11.922e-2935.44SMESG000080507.1[more]
SMESG000023281.15.162e-2739.44SMESG000023281.1[more]
SMESG000044339.14.655e-2035.06SMESG000044339.1[more]
SMESG000032554.12.651e-1828.40SMESG000032554.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005647 ID=SMED30005647|Name=SMED30005647|organism=Schmidtea mediterranea sexual|type=transcript|length=817bp
AAAATTGAACAATGAAAAGTGACGATATCTATGGGAAATCGCCAATAAAT
CGACGAGTTTCACTTGTTGGAGCCCTCGAAAGTTCGTCATGCCAAAATAC
ACCAACCAAAAAAGTACCTCAAAATCGTCGAGTTTCCTTAGCACCAGCTT
CTGGGACTTTTATTAAAGACAAACGCAGCTCAATTAATTCTGAAGGGACA
CCGAGTAAATTACGAAAGATGTCACAGTTTTTTCACGTGGATAAAATATC
AGAAACGCTTTTAAAAGATCCAGAAAAAGAGGCCTTCATTCAGATGTATG
AACAGCACGATGATTTGCAAACAGAATATAAAAGATTAAAATCGTATGTT
GAACATTTGAAAGATGACTATGAAATGAGTCGAGGATTCGAAGCTATGAC
TCGATACAACAGATTAAAAGTCATGATTAAACGGACAATAATGCATTTGA
AAGTATCTAGTGTAGCTGGAGATTGTAGCAACGCCATTAAAATAAAAGAA
TTGGCTAGAAAAAGTGAAGAAAGATATGAAAGTCTGACATCCGATGAAAT
TGACAAAGAAAAACAAGAAATAAAAGAAAACATTGAGGAATTTCAACAGA
GAATAGATATTTTGATCAGTGCTATAACGGATCTTGAGGAGCATTACAAT
GACGCAAAGACCTATTTTATATGGACTCGTTATCAAATGATGAAAGCCAT
GGTGAAGGACCTAATTCACAAACCGACGATTGTAGAAATAATTTGAAGAT
TTAGTTTATTATTATTTGTGCAGATTTTGATAAATTATTATTAATATTAT
TATAAAAATGATTTTGA
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protein sequence of SMED30005647-orf-1

>SMED30005647-orf-1 ID=SMED30005647-orf-1|Name=SMED30005647-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=245bp
MKSDDIYGKSPINRRVSLVGALESSSCQNTPTKKVPQNRRVSLAPASGTF
IKDKRSSINSEGTPSKLRKMSQFFHVDKISETLLKDPEKEAFIQMYEQHD
DLQTEYKRLKSYVEHLKDDYEMSRGFEAMTRYNRLKVMIKRTIMHLKVSS
VAGDCSNAIKIKELARKSEERYESLTSDEIDKEKQEIKENIEEFQQRIDI
LISAITDLEEHYNDAKTYFIWTRYQMMKAMVKDLIHKPTIVEII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 99..119
NoneNo IPR availableCOILSCoilCoilcoord: 177..211
NoneNo IPR availablePANTHERPTHR21010FAMILY NOT NAMEDcoord: 88..144
coord: 156..243