Vasodilator-stimulated phosphoprotein/sprouty protein

Overview
NameVasodilator-stimulated phosphoprotein/sprouty protein
Smed IDSMED30005640
Length (bp)1152
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vasodilator-stimulated phosphoprotein/sprouty protein (SMED30005640) t-SNE clustered cells

Violin plots show distribution of expression levels for Vasodilator-stimulated phosphoprotein/sprouty protein (SMED30005640) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vasodilator-stimulated phosphoprotein/sprouty protein (SMED30005640) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vasodilator-stimulated phosphoprotein/sprouty protein (SMED30005640) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005640

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005640SMESG000016725.1 SmedASXL_009752SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. TrEMBL
Match: A0A0R3X2V0 (BUD22 domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7624 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.451e-7
Identity = 41/122 (33.61%), Postives = 67/122 (54.92%), Query Frame = 3 
Query:  186 RMLIRVVS*KISKMKLIFLKRQT*IHYLKEYLPINIQLEKLGDRLSLEDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKVNKTAKKTSKAVTIPLAYGIQEIDVSKVES 551
            R+L ++   K+ K+ L  +      HYL         LE  G+ L+++DR+V+RL +  +I + I NFR+QH+DW  LV  +LYK T GK K +   ++ +K     L + +  +D  +VES
Sbjct:   77 RLLRKISDIKLCKLVLSNMN----PHYL---------LETGGENLTMQDRVVIRLAARPEISEFIRNFRTQHTDWPILVRYLLYKNTSGKWKTSDQKRRPNKRRKGDLPFPL--VDPKEVES 183
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. TrEMBL
Match: W6U5S3 (Vasodilator-stimulated phosphoprotein/sprouty protein OS=Echinococcus granulosus OX=6210 GN=EGR_08640 PE=4 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.269e-6
Identity = 28/56 (50.00%), Postives = 40/56 (71.43%), Query Frame = 3 
Query:  297 LEKLGDRLSLEDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKV 464
            LEK G+ L+ +DRIV+RL +  +I + ++NFR++HSDW  LV  +LYK T GK K 
Sbjct:  103 LEKGGEGLTKQDRIVIRLAARPEIAEFVSNFRTKHSDWPTLVQYLLYKNTSGKWKT 158
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. TrEMBL
Match: A0A0R3VUL3 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS1020 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.402e-6
Identity = 28/65 (43.08%), Postives = 44/65 (67.69%), Query Frame = 3 
Query:  297 LEKLGDRLSLEDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKVNKTAKKTSK 491
            LE+ G  LS +DRI++RL +  +I + ++NFR++H+DW  LV  +LYK T GK K  +  +K +K
Sbjct:  101 LEEGGKGLSKQDRIIIRLVARPEIAEFVSNFRTKHTDWPTLVQYLLYKNTSGKWKTPEQKRKVNK 165
[Close]
BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. TrEMBL
Match: A0A068XX24 (Vasodilator stimulated phosphoprotein:sprouty OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000415900 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.247e-6
Identity = 27/56 (48.21%), Postives = 39/56 (69.64%), Query Frame = 3 
Query:  297 LEKLGDRLSLEDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKV 464
            L+K G  L+ +DRIV+RL +  +I + ++NFR++HSDW  LV  +LYK T GK K 
Sbjct:  101 LKKGGKGLTKQDRIVIRLATRPEIAEFVSNFRTKHSDWSTLVQYLLYKNTSGKWKT 156
[Close]
BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Planmine SMEST
Match: SMESG000016725.1 (SMESG000016725.1)

HSP 1 Score: 353.599 bits (906), Expect = 5.457e-122
Identity = 208/213 (97.65%), Postives = 210/213 (98.59%), Query Frame = 3 
Query:  297 LEKLGDRLSLEDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKVNKTAKKTSKAVTIPLAYGIQEIDVSKVESEKIFITPSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVAMETFYISDENIREFIKNHDDLDEWERADNDADEIQEFDLMKPLNFSFNPIEKSASEKQVKAAKQPKKSMKRKFTHETQPKVWKKSA 935
            LEKLGDRLSL+DRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKVNKTAKKTSKAVTIPLAYGIQEIDVSKVESEKIF+TPSIREEKLPRVKKELKKGKVES PDGEKKGKPEPVAMETF ISDENIREFIKNHDDLDEWERADNDADEIQEFDLMKPLNFSFNPIEKSASEKQVKAAKQPKKSMKRKF HETQPKVWKKSA
Sbjct:   53 LEKLGDRLSLDDRIVVRLFSFNKIHQEITNFRSQHSDWEELVDVVLYKTTCGKMKVNKTAKKTSKAVTIPLAYGIQEIDVSKVESEKIFVTPSIREEKLPRVKKELKKGKVESIPDGEKKGKPEPVAMETFDISDENIREFIKNHDDLDEWERADNDADEIQEFDLMKPLNFSFNPIEKSASEKQVKAAKQPKKSMKRKFPHETQPKVWKKSA 265

HSP 2 Score: 75.8702 bits (185), Expect = 2.139e-15
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 2 
Query:  188 DADSCSKLKNKQDEVDFLEKADLDTLSKRVFANKYTT 298
            DADSCSKLKNKQDEVDFLEKADLDTLSKRVFANKYT+
Sbjct:   14 DADSCSKLKNKQDEVDFLEKADLDTLSKRVFANKYTS 50
The following BLAST results are available for this feature:
BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A0R3X2V02.451e-733.61BUD22 domain-containing protein OS=Hydatigena taen... [more]
W6U5S31.269e-650.00Vasodilator-stimulated phosphoprotein/sprouty prot... [more]
A0A0R3VUL32.402e-643.08Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
A0A068XX249.247e-648.21Vasodilator stimulated phosphoprotein:sprouty OS=E... [more]
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Vasodilator-stimulated phosphoprotein/sprouty protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016725.15.457e-12297.65SMESG000016725.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005640 ID=SMED30005640|Name=Vasodilator-stimulated phosphoprotein/sprouty protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1152bp
GCTTATCATGTTAAATATTGAAACTTTTGATATTAATGATTCGTCATGTA
AAGGTTATTCCATTGAATTCAGTAGTTTAATTGAATTTATCAAAAAACTA
AGATCACTTACTAAAATTGCAAAATTTCATATTATCAAATTATTAAATAT
GAAAATTAATAAGCTCAAGTCTAAATTAAAAGTAAAGGATGCTGATTCGT
GTAGTAAGTTGAAAAATAAGCAAGATGAAGTTGATTTTCTTGAAAAGGCA
GACTTAGATACATTATCTAAAAGAGTATTTGCCAATAAATATACAACTAG
AAAAGCTGGGGGATCGTTTGTCACTGGAAGATAGAATAGTTGTTCGATTG
TTTTCATTCAACAAAATCCATCAGGAAATAACGAATTTCAGGTCTCAGCA
TTCCGACTGGGAAGAACTGGTCGATGTCGTGTTGTATAAAACCACTTGCG
GTAAAATGAAAGTCAATAAAACGGCCAAGAAAACATCGAAAGCCGTCACA
ATTCCCCTGGCGTATGGAATCCAGGAAATTGACGTTTCCAAAGTCGAGAG
TGAAAAGATTTTCATCACTCCTTCTATCAGAGAGGAAAAACTTCCTCGTG
TTAAGAAAGAGTTAAAGAAAGGGAAAGTCGAATCAAAACCCGATGGGGAA
AAGAAGGGCAAACCCGAACCGGTTGCCATGGAAACGTTTTATATTTCCGA
TGAGAATATCAGAGAATTCATCAAGAATCACGACGATTTGGACGAGTGGG
AGCGCGCGGATAACGACGCCGATGAAATTCAGGAGTTCGATTTGATGAAG
CCGTTGAATTTCTCCTTCAATCCGATCGAGAAGTCTGCAAGTGAAAAGCA
GGTCAAAGCGGCAAAGCAACCGAAAAAATCAATGAAAAGGAAATTTACAC
ACGAAACGCAACCGAAAGTTTGGAAAAAATCTGCTTAATAAAAAATTTTG
GCTTTCATTTGATAAATTTTTATCTTGGATTTGGTTGCTAAGCAATGCAA
TTTTCCGGGATTTTTGAAATGTGTTTTATATATTGATTTGGATTACGCAA
CTGCAACGGTATTACGTGATTGGTTGTTTTGCAGTAATAATGAATACTTG
CTCTCGTTACCTATATCGGGAATGGGCAAATTGCCTCTCCTGTTCTATGA
GA
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protein sequence of SMED30005640-orf-1

>SMED30005640-orf-1 ID=SMED30005640-orf-1|Name=SMED30005640-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp
LIMLNIETFDINDSSCKGYSIEFSSLIEFIKKLRSLTKIAKFHIIKLLNM
KINKLKSKLKVKDADSCSKLKNKQDEVDFLEKADLDTLSKRVFANKYTTR
KAGGSFVTGR*
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protein sequence of SMED30005640-orf-2

>SMED30005640-orf-2 ID=SMED30005640-orf-2|Name=SMED30005640-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=161bp
MKVNKTAKKTSKAVTIPLAYGIQEIDVSKVESEKIFITPSIREEKLPRVK
KELKKGKVESKPDGEKKGKPEPVAMETFYISDENIREFIKNHDDLDEWER
ADNDADEIQEFDLMKPLNFSFNPIEKSASEKQVKAAKQPKKSMKRKFTHE
TQPKVWKKSA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 125..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..160
NoneNo IPR availableCDDcd18079S-AdoMet_syntcoord: 68..116
e-value: 0.00835348
score: 33.5309