G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30005622
Length (bp)1277
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30005622) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30005622) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30005622) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30005622) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005622

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30005622SMESG000051347.1 SmedASXL_000601SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30005622SMESG000051347.1 dd_Smed_v4_16800_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30005622SMESG000051347.1 dd_Smed_v6_16800_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3W7Y4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS6427 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 5.785e-39
Identity = 74/189 (39.15%), Postives = 121/189 (64.02%), Query Frame = 1 
Query:  106 VYVQAPLCLIGFILNLINFSIFAQKKFSAAP-YVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            VYVQ  +C +GFILN+IN  +F ++ FS+ P Y+L++A+S+ D+ITL  RI + +      S      + +  Y++A + EVPITN+TE++S W  V LA ERY+AM ++N ++QYST  N K  ++ + L+S+  N P+F I+   IE+    +  G + VI ++ ++L  S+FYT ++WFR   V++
Sbjct:   33 VYVQPCICFVGFILNIINSYVFQRRTFSSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSSVTQT-MAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFTRQYSTRQNTKRLIISIALVSVFFNLPYFMIHR--IEFRRFAN--GSMRVITNT-TALRRSQFYTVYSWFRMTTVQI 215

HSP 2 Score: 61.2326 bits (147), Expect = 5.785e-39
Identity = 51/143 (35.66%), Postives = 78/143 (54.55%), Query Frame = 3 
Query:  666 TLVWEHKKKLKQ------------FQSKRSGY--LNTKD----NNKITQRKQR---AQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLMQSV 1031
            T+V++H KK  +              ++ SG   L+ KD    ++ +TQR+Q+   AQ KL +LLI +I+LFL G IP +FSY++++E       A+ Y   CP Y +Y  V+  + +  Y   FF+Y  LN HFRN L + V
Sbjct:  230 TIVYQHNKKFGKNTEMEQKGNRLSLGAEGSGAEELSLKDTTMGDSWLTQRRQKKRNAQRKLNILLIAIILLFLFGAIPQAFSYIRIFEAFGTCKEAFHY---CPTYVIYRMVTTNIALISYGLTFFLYFFLNRHFRNELQKCV 369
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P6TEG8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS3003 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 3.428e-34
Identity = 76/188 (40.43%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  106 VYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            VYVQ  +CL+GF LN++N  IFA+K F+A  Y+L+ A+S+ DSITL  RI + ++     S  +   + +  Y++A ++EVPITN++E++S W  V+LA ER++AM ++NLSKQY    NAK  +L +T +S++LN P+F I+ + ++  +     G V ++ SS +    S FYT  +W R F+V++
Sbjct:   29 VYVQPFICLVGFALNVVNSCIFARKTFAAPAYLLMFALSISDSITLGLRIPQGMIYYRGNSMTQK-MIAIYAYIYATYVEVPITNMSENMSAWLTVSLAIERFVAMKHWNLSKQYCHRQNAKRLVLAITAISVILNLPYFMIHQLQVQKRSN----GAVRIL-SSTTRFGDSSFYTVFSWLRIFLVQI 210
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183SN57 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS5655 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.089e-33
Identity = 74/188 (39.36%), Postives = 124/188 (65.96%), Query Frame = 1 
Query:  106 VYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            VYVQ  +CL+G +LN++N  IFA+K F+   Y+L+ A+S+ DSITL  RI + ++     S  +   + + +Y++A ++EVPITN++E++S W  V+LA ER++AM ++NLSKQY    NAK  +  +T++SI+LN P+F I+ + ++  +     G V ++ SS +    S FYT ++W R F+V++
Sbjct:   29 VYVQPFICLVGLVLNIVNSCIFARKTFAPPAYLLMFALSISDSITLGLRIPQGLIYYQGNSMTQK-MIAIYVYIYATYVEVPITNMSENMSAWLTVSLAIERFVAMKHWNLSKQYCHRRNAKRLVFVITVISIILNLPYFMIHQLQVQKRSN----GAVRIV-SSTTRFGDSSFYTVYSWLRIFLVQI 210
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: W6UXS1 (Putative G-protein coupled receptor OS=Echinococcus granulosus OX=6210 GN=EGR_01868 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 1.266e-31
Identity = 77/189 (40.74%), Postives = 123/189 (65.08%), Query Frame = 1 
Query:  106 VYVQAPLCLIGFILNLINFSIFAQKKFSAAP-YVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            VYVQ  +CLIGFILN+IN  +F +K F + P Y+L++A+S+ D+ITL  RI + +      S      + +  Y++A + EVPITN+TE++S W  V LA ERY+AM ++N ++QYST  N K  ++ + L+S++ N P+F I+   IE++   +  G + VI ++ ++L  S FYT ++WFR  IV++
Sbjct:   33 VYVQPCICLIGFILNIINIYVFQRKTFCSTPAYMLVLALSIADAITLGLRIPQGLTYYRGSSSVTQT-MAIYAYIYATYAEVPITNMTENMSAWLTVCLALERYVAMRHWNFARQYSTRQNTKRLIISIALVSVLFNLPYFMIHR--IEFKRFAN--GSMRVITNA-TALRRSTFYTVYSWFRMTIVQI 215

HSP 2 Score: 61.6178 bits (148), Expect = 1.115e-6
Identity = 42/100 (42.00%), Postives = 59/100 (59.00%), Query Frame = 3 
Query:  729 NTKDNNKITQRKQR---AQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSL 1019
             T  + ++ QR+Q+   AQ KL +LLI VI+LFL G IP +FSY++++E       A+ Y   CP Y VY  V+  + +  Y   FF+Y  LN HFRN L
Sbjct:  269 TTVGDLQLIQRRQKKRNAQRKLNILLIAVILLFLFGAIPQAFSYIRIFEAFGTCEEAFHY---CPTYVVYRMVTTNIALISYGLTFFLYFFLNQHFRNEL 365
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A564XYB0 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS811 PE=4 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 1.286e-31
Identity = 73/189 (38.62%), Postives = 120/189 (63.49%), Query Frame = 1 
Query:  106 VYVQAPLCLIGFILNLINFSIFAQKKFSAAP-YVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            VY+Q  +C+IGF+LN+IN  IF +K FS+ P YVL++A+S+VD+ITL  RI + +         +   + +  Y++A + E+PITN+TE++S W  V LA ERY+AM ++N ++QY +  N    ++ + ++SI+ N P+F I+ + ++     S  G + V  SS + L  SKFY F++WFR   V++
Sbjct:   34 VYMQPWICIIGFVLNIINSYIFCRKTFSSTPAYVLVLALSIVDAITLGLRIPQGLTHYRGSQTAQT--IAINAYIYATYAEIPITNMTENMSAWLTVCLALERYVAMRHWNFARQYCSRRNTIRVIIGIAVVSIIFNLPYFMIHRINVQ----RSQNGTLKVT-SSVTELKRSKFYAFYSWFRMATVQI 215
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000051347.1 (SMESG000051347.1)

HSP 1 Score: 407.142 bits (1045), Expect = 6.390e-140
Identity = 202/203 (99.51%), Postives = 203/203 (100.00%), Query Frame = 1 
Query:   61 MSTNISSTFPYEFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            MSTNISSTFPYEFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVK+
Sbjct:    1 MSTNISSTFPYEFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKV 203

HSP 2 Score: 336.265 bits (861), Expect = 3.613e-112
Identity = 207/222 (93.24%), Postives = 210/222 (94.59%), Query Frame = 3 
Query:  615 IVKVLHISHM-VSTIHCQTLVWEHKKKLKQFQSKRSGYLNTKDNNKITQRKQRAQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLMQSVVSCFCHCNRCYRTRXXXXXXXXXXXXXXXMQSTAFLPANRXXXXXXXXXXXXVKDNIISQNSLHPNACLRTSENTIETYLCS 1277
            IVKV+ +  + V       LVWEHKKKLKQFQSKRSGYLNTKDNNKITQRKQRAQNKLTMLLIVVIVLFLIGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLMQSV SCFCHCNRCYRTRILIINENEIALNSILMQSTAFLPANRSSTLSSNSPMLSVKDNIISQNSLHPNACLRTSENTIETYLCS
Sbjct:  200 IVKVIPLLFLCVCNFLLLALVWEHKKKLKQFQSKRSGYLNTKDNNKITQRKQRAQNKLTMLLIVVIVLFLIGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLMQSVASCFCHCNRCYRTRILIINENEIALNSILMQSTAFLPANRSSTLSSNSPMLSVKDNIISQNSLHPNACLRTSENTIETYLCS 421
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000068527.1 (SMESG000068527.1)

HSP 1 Score: 192.2 bits (487), Expect = 6.944e-56
Identity = 96/205 (46.83%), Postives = 148/205 (72.20%), Query Frame = 1 
Query:   55 NSMSTNISSTFPYEFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            N  + N+ +T   E L V++Q P+C++GF LN+INFS+F ++K+ AAPYVLL A+SL D++TL+   +E + KC  L+FC+ PYLQ+  Y+FA++++ P++NITE+IS+   VALATERY+AM +YN  K Y TV N +LF+L +++ S + N+PFF+   + I WEN TS +  VPV+YS+++SL  S FY+ H+W R   +++
Sbjct:    5 NGTNDNVPTT---ELLVVFLQGPICIVGFGLNIINFSVFVKRKYFAAPYVLLSALSLADAVTLLLYFTETMAKCQHLAFCKQPYLQLSAYVFALYVKNPLSNITETISSIITVALATERYLAMRHYNFRKIYFTVKNTRLFVLLISVFSTLFNTPFFFTTTIKISWENETSTKQKVPVVYSNYTSLIDSYFYSTHSWTRAIAIQM 206

HSP 2 Score: 80.1073 bits (196), Expect = 5.677e-16
Identity = 43/97 (44.33%), Postives = 68/97 (70.10%), Query Frame = 3 
Query:  729 NTKDNNKITQRKQRAQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSL 1019
            N  + + I +RKQ AQ KLT+LL ++I L+LIGQIP + +Y+K+Y    + C+ ++ + +CP+Y +Y +V+ ++M+  Y  NFFVY  LN+HFR SL
Sbjct:  257 NQTNQSSIRERKQIAQTKLTILLSIIIFLYLIGQIPSALAYIKIYRKF-HSCDNFEVIHECPSYDMYRTVTSIMMLASYALNFFVYFFLNSHFRKSL 352
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000035759.1 (SMESG000035759.1)

HSP 1 Score: 150.984 bits (380), Expect = 3.150e-41
Identity = 76/192 (39.58%), Postives = 119/192 (61.98%), Query Frame = 1 
Query:   94 EFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            +FL +Y Q P+C+IG ILN INF +  +   S A Y+L+ A++  D+ TL+ R  +  V+C +L FC  P L+  +Y+F ++ EVPITNITE++S W  +ALA E YI M ++  S+ Y TV N+K  +  +T LS ++N PFF++    +++E    +Q  +PV+Y+       S FY  H+W R  IV++
Sbjct:   20 DFLLIYFQFPICIIGSILNFINFLVLTRIGISNATYILMSALAFTDASTLLLRTVQTYVRCETLPFCEMPNLKYKLYIFVLYAEVPITNITENLSCWITLALAVEHYIQMKFFTKSRLYCTVENSKKLIFLLTFLSTLVNLPFFFLMTTSVKYETNQRNQS-IPVVYAKKRQFMDSIFYPIHSWLRFCIVQM 210

HSP 2 Score: 69.707 bits (169), Expect = 9.312e-13
Identity = 45/91 (49.45%), Postives = 66/91 (72.53%), Query Frame = 3 
Query:  750 ITQRKQRAQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLM 1022
            +  +KQ+AQ KLT+LLIV+I+LFLIGQIP +F+Y+ ++    N C    YL DCP +++Y SV+ +L + CY  NFFVY+ LN+H++NS  
Sbjct:  280 LISKKQKAQYKLTILLIVIIILFLIGQIPHAFAYVGIHRFFSN-CKDLTYLKDCPKHELYRSVTMILTLACYSLNFFVYIFLNSHYQNSFF 369
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000044466.1 (SMESG000044466.1)

HSP 1 Score: 150.984 bits (380), Expect = 4.215e-41
Identity = 71/190 (37.37%), Postives = 123/190 (64.74%), Query Frame = 1 
Query:   97 FLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLLMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVK 666
            FL  Y+Q P+CLIGF LN++N  +F +    ++ Y L+ +++L D++TLI R S+ ++       C +P L+M +Y+F +F+E P+TNITE+ISNW  ++LA ER++AM +++ SK Y T+ N K+   F+ ++++++N P+F+   V+++ E     Q     IY++ S  +LS+ Y  H+W R  IV+
Sbjct:   27 FLSFYIQIPICLIGFCLNVVNVIVFYKNIHHSSLYFLVSSLALADAVTLILRASQGLLGKECWPLCSSPQLRMYVYMFVIFLEFPVTNITETISNWLTLSLAIERFLAMKHHSFSKVYCTLKNFKILTSFICIIALIINLPYFFTYNVILKKEYNNESQMYEKAIYTNESHFSLSQIYEVHSWIRQVIVQ 216

HSP 2 Score: 66.2402 bits (160), Expect = 1.322e-11
Identity = 42/95 (44.21%), Postives = 65/95 (68.42%), Query Frame = 3 
Query:  735 KDNNKITQRKQRAQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSL 1019
            K+ +   ++K RAQ KLT+LLI VI+LFLIGQIP SF+Y+K+Y+ +       K+  +   Y+++ + + +L + CY  NFF+YL LN HFR+S+
Sbjct:  275 KEKSTAIEKKLRAQKKLTILLITVILLFLIGQIPHSFAYIKIYKAVSKYS---KFDPEGFEYRMFRTSTMVLTLLCYSLNFFIYLFLNTHFRHSV 366
[Close]
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000035759.1 (SMESG000035759.1)

HSP 1 Score: 117.087 bits (292), Expect = 2.944e-29
Identity = 58/154 (37.66%), Postives = 94/154 (61.04%), Query Frame = 1 
Query:  208 LMAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITNITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVLNSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFIVKL 669
            + A++  D+ TL+ R  +  V+C +L FC  P L+  +Y+F ++ EVPITNITE++S W  +ALA E YI M ++  S+ Y TV N+K  +  +T LS ++N PFF++    +++E    +Q  +PV+Y+       S FY  H+W R  IV++
Sbjct:    1 MSALAFTDASTLLLRTVQTYVRCETLPFCEMPNLKYKLYIFVLYAEVPITNITENLSCWITLALAVEHYIQMKFFTKSRLYCTVENSKKLIFLLTFLSTLVNLPFFFLMTTSVKYETNQRNQS-IPVVYAKKRQFMDSIFYPIHSWLRFCIVQM 153

HSP 2 Score: 70.0922 bits (170), Expect = 4.819e-13
Identity = 45/91 (49.45%), Postives = 66/91 (72.53%), Query Frame = 3 
Query:  750 ITQRKQRAQNKLTMXXXXXXXXXXXGQIPVSFSYLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALNNHFRNSLM 1022
            +  +KQ+AQ KLT+LLIV+I+LFLIGQIP +F+Y+ ++    N C    YL DCP +++Y SV+ +L + CY  NFFVY+ LN+H++NS  
Sbjct:  223 LISKKQKAQYKLTILLIVIIILFLIGQIPHAFAYVGIHRFFSN-CKDLTYLKDCPKHELYRSVTMILTLACYSLNFFVYIFLNSHYQNSFF 312
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3W7Y45.785e-3939.15G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P6TEG83.428e-3440.43G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183SN571.089e-3339.36G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
W6UXS11.266e-3140.74Putative G-protein coupled receptor OS=Echinococcu... [more]
A0A564XYB01.286e-3138.62G_PROTEIN_RECEP_F1_2 domain-containing protein (Fr... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000051347.16.390e-14099.51SMESG000051347.1[more]
SMESG000068527.16.944e-5646.83SMESG000068527.1[more]
SMESG000035759.13.150e-4139.58SMESG000035759.1[more]
SMESG000044466.14.215e-4137.37SMESG000044466.1[more]
SMESG000035759.12.944e-2937.66SMESG000035759.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005622 ID=SMED30005622|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1277bp
GTTTGGGTTTTTTGTACGTTAACAAATTTGAAAGTAATATTGTAGAAGAA
CAAAAATAGTATGTCGACTAACATATCTTCGACATTTCCATATGAATTCC
TGGACGTTTATGTTCAAGCTCCATTGTGTTTGATCGGTTTCATTTTGAAT
TTGATTAATTTCTCAATATTTGCCCAGAAAAAGTTTTCAGCTGCACCTTA
CGTTTTACTGATGGCCATGTCACTAGTGGATTCCATCACTTTGATATTCA
GAATTTCCGAAGCTGTTGTCAAGTGCACCAGCCTTTCATTTTGTCGGAAT
CCTTATTTACAAATGGTAATGTATCTGTTTGCAGTTTTCATAGAGGTTCC
TATTACTAACATAACGGAGTCCATATCAAACTGGACCGCGGTGGCCTTGG
CCACGGAGCGCTACATTGCAATGTGTTATTATAATCTCAGCAAGCAATAT
TCAACTGTTTATAATGCGAAATTATTTCTCTTATTCGTTACATTGTTATC
AATAGTGCTAAATTCTCCGTTTTTCTACATTAATTATGTCGTTATCGAAT
GGGAAAATACGACAAGTCATCAGGGAATGGTGCCAGTAATTTACAGTTCT
TTCAGTTCCTTGGCATTGTCAAAGTTCTACACATTTCACACATGGTTTCG
ACCATTCATTGTCAAACTCTCGTTTGGGAACACAAAAAGAAATTGAAACA
ATTTCAATCGAAAAGATCGGGCTATTTAAACACGAAAGACAACAACAAAA
TTACCCAGAGAAAACAACGGGCACAAAACAAATTGACAATGCTACTGATA
GTTGTCATTGTTTTGTTTCTCATCGGACAGATTCCGGTTTCTTTTTCTTA
TCTGAAAGTCTATGAAAATCTCCCTAACAGATGCAATGCCTATAAGTACC
TAACAGACTGCCCTAATTACAAGGTTTACAGCAGTGTCAGTCATCTGTTG
ATGATAACGTGCTATTGCACAAATTTCTTTGTATATTTGGCTTTGAATAA
TCACTTCAGAAACTCCCTGATGCAATCTGTTGTCAGCTGCTTCTGTCATT
GCAACCGATGTTATAGAACGAGAATATTAATAATAAACGAAAATGAAATC
GCTCTTAATTCTATTCTGATGCAGTCAACAGCCTTTCTGCCAGCAAATCG
CAGCTCTACGTTGTCGTCGAATTCTCCAATGCTTTCTGTCAAAGACAATA
TCATCTCTCAAAATTCACTTCATCCCAACGCATGCCTGAGGACTTCTGAA
AATACTATTGAAACTTATTTATGCAGC
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protein sequence of SMED30005622-orf-1

>SMED30005622-orf-1 ID=SMED30005622-orf-1|Name=SMED30005622-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=212bp
MSTNISSTFPYEFLDVYVQAPLCLIGFILNLINFSIFAQKKFSAAPYVLL
MAMSLVDSITLIFRISEAVVKCTSLSFCRNPYLQMVMYLFAVFIEVPITN
ITESISNWTAVALATERYIAMCYYNLSKQYSTVYNAKLFLLFVTLLSIVL
NSPFFYINYVVIEWENTTSHQGMVPVIYSSFSSLALSKFYTFHTWFRPFI
VKLSFGNTKRN*
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protein sequence of SMED30005622-orf-2

>SMED30005622-orf-2 ID=SMED30005622-orf-2|Name=SMED30005622-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MGKYDKSSGNGASNLQFFQFLGIVKVLHISHMVSTIHCQTLVWEHKKKLK
QFQSKRSGYLNTKDNNKITQRKQRAQNKLTMLLIVVIVLFLIGQIPVSFS
YLKVYENLPNRCNAYKYLTDCPNYKVYSSVSHLLMITCYCTNFFVYLALN
NHFRNSLMQSVVSCFCHCNRCYRTRILIINENEIALNSILMQSTAFLPAN
RSSTLSSNSPMLSVKDNIISQNSLHPNACLRTSENTIETYLCS
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
GO:0008528G-protein coupled peptide receptor activity
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 7..175
e-value: 8.0E-12
score: 46.6
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 47..172
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 47..157
e-value: 1.96636E-10
score: 57.6428
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 31..172
NoneNo IPR availableTMHMMTMhelixcoord: 79..101
NoneNo IPR availableTMHMMTMhelixcoord: 127..149