Delta-1-pyrroline-5-carboxylate synthase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005571 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 23Note: Hover over icons to view figure legend
Homology
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Human
Match: ALDH18A1 (aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]) HSP 1 Score: 828.935 bits (2140), Expect = 0.000e+0 Identity = 416/731 (56.91%), Postives = 547/731 (74.83%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGVIS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + +++IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +AV LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVDNV DAIDHI +GS HTD I+TE+++TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRGK H V DFS+ G + H++L
Sbjct: 58 GKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAAPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENL 785
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Human
Match: ALDH18A1 (aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722]) HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0 Identity = 416/733 (56.75%), Postives = 547/733 (74.62%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGV IS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + +++IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +AV LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVDNV DAIDHI +GS HTD I+TE+++TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRGK H V DFS+ G + H++L
Sbjct: 58 GKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAAPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENL 787
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Celegans
Match: alh-13 (Probable delta-1-pyrroline-5-carboxylate synthase Glutamate 5-kinase Gamma-glutamyl phosphate reductase [Source:UniProtKB/Swiss-Prot;Acc:P54889]) HSP 1 Score: 801.971 bits (2070), Expect = 0.000e+0 Identity = 410/744 (55.11%), Postives = 535/744 (71.91%), Query Frame = 2
Query: 101 SPASVHNKNFNTSNILGR-VASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTP-QEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLN-SDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSL 2320
+P V N N+ T + ++R+DLK ++R+VVKLGSAVITR DECGLALGRLASIVEQ+SE+ Q G+++++V+SGAVAFG+QKL + M+MS++QTL+ ++ D RACAA+GM GLMSLYEQLFQQYG+ A++LLTKPD +DQ R NL+ T+ LL LNIIPI+N NDAVAP P L+ + I DNDSLAAR++AE+ +LLII+S+V+G+Y+ PP+ EGS L+ TY + S + FG S+ GTGGMESK A AL G++ VI G ++A+ + V GKK+GT F T E +E AE R A +L ++ +R +R LA LL++ + I+ AN D AK + LD +L+RLK+T K+Q L GLN IADS + L+ RVL+K +++ L LE++T+PIG L+VIFESRPDCLPQVASLA+ASGN +LLKGG EA SNK L LV++AL + G DAV LV RED+ LLQL IDL+IPRGSS ++ ++ SK IPVLGH+EGVCHV++D+ + + A++IV+DSKCDYP+ACNA ET+LIHK L FF+ L M V++ +GPKLA LKF A M EYGSL CT+EVVDNV +A+ HI +GS HT++IITEN +TA FL++VDSAC FHNASTRFADGYRFGLGAEVGIST RIHARGPVGV+GLLT+KW+LRG+GH V+DF GK+S+ H++L
Sbjct: 50 TPVGVGNGNYCTKTRQKHPLINTRNDLKKAQRVVVKLGSAVITREDECGLALGRLASIVEQVSELQQSGRQMLIVSSGAVAFGRQKLRQELVMSMSMRQTLRGPSGMT-----ADKRACAASGMPGLMSLYEQLFQQYGITVAQVLLTKPDIDDDQRRKNLQATIESLLSLNIIPIVNANDAVAPDPKLN------MHISDNDSLAARLSAEIEAELLIILSNVNGVYTGPPDLEGSRLLYTYVPSENS-GVTFGANSKFGTGGMESKVTACVNALNNGVTTVITNGLAQDAITDAVAGKKIGTMFCNTKGYEGPPIEEVAEKCRDAGRQLAALSNKERGAMVRHLAALLVDKEKYIIEANQTDLANAKSAGLDPQLLNRLKMTPEKIQDLHAGLNTIADSAETLVGRVLKKVKISEGLFLEQVTVPIGSLMVIFESRPDCLPQVASLAMASGNALLLKGGKEAEESNKALHALVQEALGTHGFEMRDAVTLVRSREDVADLLQLKDLIDLIIPRGSSDLVRSMQEKSKGIPVLGHAEGVCHVYIDKDCDEQKAIQIVRDSKCDYPSACNAAETILIHKDLATAPFFDSLCSMFKAEGVKLHAGPKLAALLKFAPPP-AESMSFEYGSLECTLEVVDNVEEAVAHIIRYGSGHTESIITENTNTAEHFLKHVDSACAFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRGEGHLVEDFKNGKYSYLHENL 780
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Celegans
Match: alh-13 (Probable delta-1-pyrroline-5-carboxylate synthase Glutamate 5-kinase Gamma-glutamyl phosphate reductase [Source:UniProtKB/Swiss-Prot;Acc:P54889]) HSP 1 Score: 800.816 bits (2067), Expect = 0.000e+0 Identity = 410/746 (54.96%), Postives = 536/746 (71.85%), Query Frame = 2
Query: 101 SPASVHNKNFNTSNILGR---VASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTP-QEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLN-SDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSL 2320
+P V N N+ S + + ++R+DLK ++R+VVKLGSAVITR DECGLALGRLASIVEQ+SE+ Q G+++++V+SGAVAFG+QKL + M+MS++QTL+ ++ D RACAA+GM GLMSLYEQLFQQYG+ A++LLTKPD +DQ R NL+ T+ LL LNIIPI+N NDAVAP P L+ + I DNDSLAAR++AE+ +LLII+S+V+G+Y+ PP+ EGS L+ TY + S + FG S+ GTGGMESK A AL G++ VI G ++A+ + V GKK+GT F T E +E AE R A +L ++ +R +R LA LL++ + I+ AN D AK + LD +L+RLK+T K+Q L GLN IADS + L+ RVL+K +++ L LE++T+PIG L+VIFESRPDCLPQVASLA+ASGN +LLKGG EA SNK L LV++AL + G DAV LV RED+ LLQL IDL+IPRGSS ++ ++ SK IPVLGH+EGVCHV++D+ + + A++IV+DSKCDYP+ACNA ET+LIHK L FF+ L M V++ +GPKLA LKF A M EYGSL CT+EVVDNV +A+ HI +GS HT++IITEN +TA FL++VDSAC FHNASTRFADGYRFGLGAEVGIST RIHARGPVGV+GLLT+KW+LRG+GH V+DF GK+S+ H++L
Sbjct: 50 TPVGVGNGNYCYSTKTRQKHPLINTRNDLKKAQRVVVKLGSAVITREDECGLALGRLASIVEQVSELQQSGRQMLIVSSGAVAFGRQKLRQELVMSMSMRQTLRGPSGMT-----ADKRACAASGMPGLMSLYEQLFQQYGITVAQVLLTKPDIDDDQRRKNLQATIESLLSLNIIPIVNANDAVAPDPKLN------MHISDNDSLAARLSAEIEAELLIILSNVNGVYTGPPDLEGSRLLYTYVPSENS-GVTFGANSKFGTGGMESKVTACVNALNNGVTTVITNGLAQDAITDAVAGKKIGTMFCNTKGYEGPPIEEVAEKCRDAGRQLAALSNKERGAMVRHLAALLVDKEKYIIEANQTDLANAKSAGLDPQLLNRLKMTPEKIQDLHAGLNTIADSAETLVGRVLKKVKISEGLFLEQVTVPIGSLMVIFESRPDCLPQVASLAMASGNALLLKGGKEAEESNKALHALVQEALGTHGFEMRDAVTLVRSREDVADLLQLKDLIDLIIPRGSSDLVRSMQEKSKGIPVLGHAEGVCHVYIDKDCDEQKAIQIVRDSKCDYPSACNAAETILIHKDLATAPFFDSLCSMFKAEGVKLHAGPKLAALLKFAPPP-AESMSFEYGSLECTLEVVDNVEEAVAHIIRYGSGHTESIITENTNTAEHFLKHVDSACAFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRGEGHLVEDFKNGKYSYLHENL 782
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Fly
Match: CG7470 (gene:FBgn0037146 transcript:FBtr0309869) HSP 1 Score: 799.275 bits (2063), Expect = 0.000e+0 Identity = 408/729 (55.97%), Postives = 541/729 (74.21%), Query Frame = 2
Query: 161 SSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSD-----LQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQV--ELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
+ RS LK ++R+VVKLGSAVITR D GLALGRLASIVEQ++E +G+E+++VTSGAVAFGKQKL + M++S+++TL ++PRA AA G SGLMSLY+ +F QYG++ A++L+TKPDFYN++TR NL TL+EL+ LNI+PIINTNDAV+PP + D + I IKDNDSL+A +AAE+ DLLI+MSDVDG+Y++PP E+G+ L+ TY T+ S ++EFG KS+VGTGGM+SK KAA WAL +G+SVVIC G E A+ I+ G+KVGTFFT + N V E+ AE AR S ++ T QRA+++ LADLL+ + IL AN +D A+ S L + +LSRL L +KL+ L++GL QIA+ + RVLR+T LA L L+++T+PIGVLLVIFESRPD LPQVA+LA+AS NG+LLKGG EA +SNK L +LVK+AL + G AV+LVS RE+I LL + +IDL+IPRGSS ++ I+ S IPVLGH+EGVCHV++D A+LE ALRI +D+KCDYPAACNAMETLLIH+ L+ F + +ML + V+I++GP+L + L F A +K EYG+L C +EVV ++++AI+HI +GS HTD I+TEND+ A FL +VDSACVFHNAS+RFADG+RFGLGAEVGIST+RIHARGPVGV+GLLT+KWIL G+ H DF++ G ++ H++L
Sbjct: 48 TERSQLKYARRLVVKLGSAVITREDNHGLALGRLASIVEQVAECHLEGREVMMVTSGAVAFGKQKLAQELLMSLSMRETLNPKDSKEFDGATLEPRAAAAVGQSGLMSLYDAMFAQYGVKIAQVLVTKPDFYNEETRNNLFCTLSELISLNIVPIINTNDAVSPPMFIRDDEPAGGARRGIPIKDNDSLSAMLAAEVQADLLILMSDVDGIYNKPPWEDGAKLMHTY-TSDDSNSIEFGKKSKVGTGGMDSKVKAATWALDRGVSVVICNGMQEKAIKTIIGGRKVGTFFTEATESANAVPVEVMAENARTGSRQMQALTPAQRASAVNTLADLLVSREKFILDANAKDLAEAQKSGLAKPLLSRLSLNPAKLKNLSVGLKQIAEDSHKNVGRVLRRTRLADQLELKQVTVPIGVLLVIFESRPDSLPQVAALAMASANGLLLKGGKEAAHSNKALMELVKEALATVGAEH-AVSLVSTREEISDLLSMENHIDLIIPRGSSDLVRSIQQQSLHIPVLGHAEGVCHVYIDRDADLEKALRIARDAKCDYPAACNAMETLLIHEDLMSGAIFGDVCNMLKREGVKIYAGPRLNQQLTF-GPPAAKSLKHEYGALECCIEVVPSLDEAINHIHTYGSSHTDVIVTENDAAARQFLGSVDSACVFHNASSRFADGFRFGLGAEVGISTARIHARGPVGVEGLLTTKWILEGQDHAAADFAEGGGRTWLHETL 773
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Fly
Match: CG7470 (gene:FBgn0037146 transcript:FBtr0078477) HSP 1 Score: 799.275 bits (2063), Expect = 0.000e+0 Identity = 408/729 (55.97%), Postives = 541/729 (74.21%), Query Frame = 2
Query: 161 SSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSD-----LQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQV--ELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
+ RS LK ++R+VVKLGSAVITR D GLALGRLASIVEQ++E +G+E+++VTSGAVAFGKQKL + M++S+++TL ++PRA AA G SGLMSLY+ +F QYG++ A++L+TKPDFYN++TR NL TL+EL+ LNI+PIINTNDAV+PP + D + I IKDNDSL+A +AAE+ DLLI+MSDVDG+Y++PP E+G+ L+ TY T+ S ++EFG KS+VGTGGM+SK KAA WAL +G+SVVIC G E A+ I+ G+KVGTFFT + N V E+ AE AR S ++ T QRA+++ LADLL+ + IL AN +D A+ S L + +LSRL L +KL+ L++GL QIA+ + RVLR+T LA L L+++T+PIGVLLVIFESRPD LPQVA+LA+AS NG+LLKGG EA +SNK L +LVK+AL + G AV+LVS RE+I LL + +IDL+IPRGSS ++ I+ S IPVLGH+EGVCHV++D A+LE ALRI +D+KCDYPAACNAMETLLIH+ L+ F + +ML + V+I++GP+L + L F A +K EYG+L C +EVV ++++AI+HI +GS HTD I+TEND+ A FL +VDSACVFHNAS+RFADG+RFGLGAEVGIST+RIHARGPVGV+GLLT+KWIL G+ H DF++ G ++ H++L
Sbjct: 48 TERSQLKYARRLVVKLGSAVITREDNHGLALGRLASIVEQVAECHLEGREVMMVTSGAVAFGKQKLAQELLMSLSMRETLNPKDSKEFDGATLEPRAAAAVGQSGLMSLYDAMFAQYGVKIAQVLVTKPDFYNEETRNNLFCTLSELISLNIVPIINTNDAVSPPMFIRDDEPAGGARRGIPIKDNDSLSAMLAAEVQADLLILMSDVDGIYNKPPWEDGAKLMHTY-TSDDSNSIEFGKKSKVGTGGMDSKVKAATWALDRGVSVVICNGMQEKAIKTIIGGRKVGTFFTEATESANAVPVEVMAENARTGSRQMQALTPAQRASAVNTLADLLVSREKFILDANAKDLAEAQKSGLAKPLLSRLSLNPAKLKNLSVGLKQIAEDSHKNVGRVLRRTRLADQLELKQVTVPIGVLLVIFESRPDSLPQVAALAMASANGLLLKGGKEAAHSNKALMELVKEALATVGAEH-AVSLVSTREEISDLLSMENHIDLIIPRGSSDLVRSIQQQSLHIPVLGHAEGVCHVYIDRDADLEKALRIARDAKCDYPAACNAMETLLIHEDLMSGAIFGDVCNMLKREGVKIYAGPRLNQQLTF-GPPAAKSLKHEYGALECCIEVVPSLDEAINHIHTYGSSHTDVIVTENDAAARQFLGSVDSACVFHNASSRFADGFRFGLGAEVGISTARIHARGPVGVEGLLTTKWILEGQDHAAADFAEGGGRTWLHETL 773
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Zebrafish
Match: aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-5602]) HSP 1 Score: 808.905 bits (2088), Expect = 0.000e+0 Identity = 420/737 (56.99%), Postives = 549/737 (74.49%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT----LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+L+ +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + L D+S P ++ RACAAAG SGLM+LYE +F QY TA+IL+T DF+++Q R NL TL ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DLLI +SDV+GLY PP + + L+DT+ ++ +G KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR A L QR++ I LADLL E + +IL AN +D A + L ML RL L+SSKL +L+IGL QIA S + + RVLR+T +A +L LE+IT+PIGVLLVIFESRPDCLPQV++LAIASGN +LLKGG EA N+N+ L +L ++AL G+ DA+ LVS RE+++ L +L K IDL+IPRGSSQ++ I+ +K IPVLGHSEG+CHV+VD A+++ A++I++DSKCDYPAACNAMETLL+H+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L C +EVVD++ +AIDHI +GS HTD I+TEN++TA FLQ +DSACVF NAS+RFADGYRFGLGAEVGIST+RIHARGPVG++GLLT+KW+LRG+GH V DFS+ G ++ H++L
Sbjct: 38 GKSFAHRSELRQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMS--LPVLEARACAAAGQSGLMALYEAMFTQYSTCTAQILVTNLDFHDEQKRRNLNSTLYELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMRADLLIALSDVEGLYDSPPGSDDAKLLDTFYPGD-QQSITYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTHPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARSAGRTLASLEPEQRSDIICALADLLTERKEEILSANKKDMENAVNTGRLSPAMLKRLSLSSSKLNSLSIGLRQIAVSSQDSVGRVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVSALAIASGNALLLKGGKEAANTNRILHELAQEALSIHGVK-DAIQLVSTREEVEDLCRLEKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGICHVYVDHEASIDKAIKIIRDSKCDYPAACNAMETLLVHRDLLRTPVFDQIIDMLRTEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLECCIEVVDSMQEAIDHIHKYGSSHTDVIVTENENTAEQFLQQLDSACVFWNASSRFADGYRFGLGAEVGISTARIHARGPVGLEGLLTTKWVLRGEGHTVADFSEHGSMTYLHENL 769
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Zebrafish
Match: aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-5602]) HSP 1 Score: 808.905 bits (2088), Expect = 0.000e+0 Identity = 420/737 (56.99%), Postives = 549/737 (74.49%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT----LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+L+ +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + L D+S P ++ RACAAAG SGLM+LYE +F QY TA+IL+T DF+++Q R NL TL ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DLLI +SDV+GLY PP + + L+DT+ ++ +G KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR A L QR++ I LADLL E + +IL AN +D A + L ML RL L+SSKL +L+IGL QIA S + + RVLR+T +A +L LE+IT+PIGVLLVIFESRPDCLPQV++LAIASGN +LLKGG EA N+N+ L +L ++AL G+ DA+ LVS RE+++ L +L K IDL+IPRGSSQ++ I+ +K IPVLGHSEG+CHV+VD A+++ A++I++DSKCDYPAACNAMETLL+H+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L C +EVVD++ +AIDHI +GS HTD I+TEN++TA FLQ +DSACVF NAS+RFADGYRFGLGAEVGIST+RIHARGPVG++GLLT+KW+LRG+GH V DFS+ G ++ H++L
Sbjct: 38 GKSFAHRSELRQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMS--LPVLEARACAAAGQSGLMALYEAMFTQYSTCTAQILVTNLDFHDEQKRRNLNSTLYELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMRADLLIALSDVEGLYDSPPGSDDAKLLDTFYPGD-QQSITYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTHPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARSAGRTLASLEPEQRSDIICALADLLTERKEEILSANKKDMENAVNTGRLSPAMLKRLSLSSSKLNSLSIGLRQIAVSSQDSVGRVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVSALAIASGNALLLKGGKEAANTNRILHELAQEALSIHGVK-DAIQLVSTREEVEDLCRLEKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGICHVYVDHEASIDKAIKIIRDSKCDYPAACNAMETLLVHRDLLRTPVFDQIIDMLRTEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLECCIEVVDSMQEAIDHIHKYGSSHTDVIVTENENTAEQFLQQLDSACVFWNASSRFADGYRFGLGAEVGISTARIHARGPVGLEGLLTTKWVLRGEGHTVADFSEHGSMTYLHENL 769
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:550060]) HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0 Identity = 429/762 (56.30%), Postives = 553/762 (72.57%), Query Frame = 2
Query: 104 PASVHNKNFNTSNIL----------GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT----LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLA--KYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
P S H + SNI G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + L D+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DL+I++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE QAE+AR L QRA I LADLL +H+ +IL AN +D A K L +L RL L+++KL +L+IGL QIA S + + RVLR+T +A DL LEE+T+PIGVLLVIFESRPDCLPQV++LAIASGNG+LLKGG EA N+N+ L++L ++AL G+ DAV LV+ RE+++ L +LNK IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CHV+VD A+++ RIV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD V DAI+HI +GS HTD IITEN+ TA FLQ+VDSACVF N STRF+DGYRFGLGAEVGIST+RIHARGPVG++GLLT+KWILRG+ H V DFS QG + H+ L
Sbjct: 34 PGSTHQTARHWSNIPFITVPHMRSHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMQV--PVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMKADLIIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLATLLPEQRAEIIFHLADLLTDHREEILSANKKDMDEAEDKGRLAAPLLKRLSLSTAKLNSLSIGLRQIAASSQDSVGRVLRRTRIAKDLELEEVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEAANTNQILYRLTQEALSLHGVR-DAVQLVNTREEVEDLCRLNKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHVYVDSDASVDKVTRIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRVEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCLEVVDGVQDAIEHIHKYGSSHTDAIITENEQTAEYFLQHVDSACVFWNTSTRFSDGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWILRGENHVVSDFSEQGSKKYLHEKL 790
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:550060]) HSP 1 Score: 802.742 bits (2072), Expect = 0.000e+0 Identity = 414/756 (54.76%), Postives = 535/756 (70.77%), Query Frame = 2
Query: 104 PASVHNKNFNTSNIL----------GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLA--KYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
P S H + SNI G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + + + P + R C M L Y +IL+T DF+++Q R NL GTL+ELL +NI+PIINTNDAV PPP+ +SDLQGVISIKDNDSLAAR+A EM DL+I++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE QAE+AR L QRA I LADLL +H+ +IL AN +D A K L +L RL L+++KL +L+IGL QIA S + + RVLR+T +A DL LEE+T+PIGVLLVIFESRPDCLPQV++LAIASGNG+LLKGG EA N+N+ L++L ++AL G+ DAV LV+ RE+++ L +LNK IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CHV+VD A+++ RIV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD V DAI+HI +GS HTD IITEN+ TA FLQ+VDSACVF N STRF+DGYRFGLGAEVGIST+RIHARGPVG++GLLT+KWILRG+ H V DFS QG + H+ L
Sbjct: 44 PGSTHQTARHWSNIPFITVPHMRSHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMPTIYVRNCIPCTMC--------LPDSYLFWCFQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVISIKDNDSLAARLAVEMKADLIIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLATLLPEQRAEIIFHLADLLTDHREEILSANKKDMDEAEDKGRLAAPLLKRLSLSTAKLNSLSIGLRQIAASSQDSVGRVLRRTRIAKDLELEEVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEAANTNQILYRLTQEALSLHGVR-DAVQLVNTREEVEDLCRLNKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHVYVDSDASVDKVTRIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRVEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCLEVVDGVQDAIEHIHKYGSSHTDAIITENEQTAEYFLQHVDSACVFWNTSTRFSDGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWILRGENHVVSDFSEQGSKKYLHEKL 788
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:550060]) HSP 1 Score: 796.964 bits (2057), Expect = 0.000e+0 Identity = 417/765 (54.51%), Postives = 538/765 (70.33%), Query Frame = 2
Query: 83 RFLNRISPASVHNKNFNTSNIL----------GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLA--KYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
RF N P S H + SNI G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + + + P + R C M L Y +IL+T DF+++Q R NL GTL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DL+I++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE QAE+AR L QRA I LADLL +H+ +IL AN +D A K L +L RL L+++KL +L+IGL QIA S + + RVLR+T +A DL LEE+T+PIGVLLVIFESRPDCLPQV++LAIASGNG+LLKGG EA N+N+ L++L ++AL G+ DAV LV+ RE+++ L +LNK IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CHV+VD A+++ RIV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD V DAI+HI +GS HTD IITEN+ TA FLQ+VDSACVF N STRF+DGYRFGLGAEVGIST+RIHARGPVG++GLLT+KWILRG+ H V DFS QG + H+ L
Sbjct: 7 RFGN--FPGSTHQTARHWSNIPFITVPHMRSHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMPTIYVRNCIPCTMC--------LPDSYLFWCFQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMKADLIIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLATLLPEQRAEIIFHLADLLTDHREEILSANKKDMDEAEDKGRLAAPLLKRLSLSTAKLNSLSIGLRQIAASSQDSVGRVLRRTRIAKDLELEEVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEAANTNQILYRLTQEALSLHGVR-DAVQLVNTREEVEDLCRLNKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHVYVDSDASVDKVTRIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRVEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCLEVVDGVQDAIEHIHKYGSSHTDAIITENEQTAEYFLQHVDSACVFWNTSTRFSDGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWILRGENHVVSDFSEQGSKKYLHEKL 758
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:550060]) HSP 1 Score: 796.193 bits (2055), Expect = 0.000e+0 Identity = 414/758 (54.62%), Postives = 535/758 (70.58%), Query Frame = 2
Query: 104 PASVHNKNFNTSNIL----------GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLA--KYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
P S H + SNI G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + + + P + R C M L Y +IL+T DF+++Q R NL GTL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DL+I++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE QAE+AR L QRA I LADLL +H+ +IL AN +D A K L +L RL L+++KL +L+IGL QIA S + + RVLR+T +A DL LEE+T+PIGVLLVIFESRPDCLPQV++LAIASGNG+LLKGG EA N+N+ L++L ++AL G+ DAV LV+ RE+++ L +LNK IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CHV+VD A+++ RIV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD V DAI+HI +GS HTD IITEN+ TA FLQ+VDSACVF N STRF+DGYRFGLGAEVGIST+RIHARGPVG++GLLT+KWILRG+ H V DFS QG + H+ L
Sbjct: 31 PGSTHQTARHWSNIPFITVPHMRSHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMPTIYVRNCIPCTMC--------LPDSYLFWCFQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMKADLIIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLATLLPEQRAEIIFHLADLLTDHREEILSANKKDMDEAEDKGRLAAPLLKRLSLSTAKLNSLSIGLRQIAASSQDSVGRVLRRTRIAKDLELEEVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEAANTNQILYRLTQEALSLHGVR-DAVQLVNTREEVEDLCRLNKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHVYVDSDASVDKVTRIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRVEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCLEVVDGVQDAIEHIHKYGSSHTDAIITENEQTAEYFLQHVDSACVFWNTSTRFSDGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWILRGENHVVSDFSEQGSKKYLHEKL 777
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:550060]) HSP 1 Score: 796.193 bits (2055), Expect = 0.000e+0 Identity = 414/758 (54.62%), Postives = 535/758 (70.58%), Query Frame = 2
Query: 104 PASVHNKNFNTSNIL----------GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLA--KYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
P S H + SNI G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + + + P + R C M L Y +IL+T DF+++Q R NL GTL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DL+I++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE QAE+AR L QRA I LADLL +H+ +IL AN +D A K L +L RL L+++KL +L+IGL QIA S + + RVLR+T +A DL LEE+T+PIGVLLVIFESRPDCLPQV++LAIASGNG+LLKGG EA N+N+ L++L ++AL G+ DAV LV+ RE+++ L +LNK IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CHV+VD A+++ RIV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD V DAI+HI +GS HTD IITEN+ TA FLQ+VDSACVF N STRF+DGYRFGLGAEVGIST+RIHARGPVG++GLLT+KWILRG+ H V DFS QG + H+ L
Sbjct: 35 PGSTHQTARHWSNIPFITVPHMRSHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMPTIYVRNCIPCTMC--------LPDSYLFWCFQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMKADLIIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKISGHVITDIVEGKKVGTFFSEVKPAGPTVEQQAEMARTGGRSLATLLPEQRAEIIFHLADLLTDHREEILSANKKDMDEAEDKGRLAAPLLKRLSLSTAKLNSLSIGLRQIAASSQDSVGRVLRRTRIAKDLELEEVTVPIGVLLVIFESRPDCLPQVSALAIASGNGLLLKGGKEAANTNQILYRLTQEALSLHGVR-DAVQLVNTREEVEDLCRLNKLIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHVYVDSDASVDKVTRIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRVEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCLEVVDGVQDAIEHIHKYGSSHTDAIITENEQTAEYFLQHVDSACVFWNTSTRFSDGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWILRGENHVVSDFSEQGSKKYLHEKL 781
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Match: Aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:MGI Symbol;Acc:MGI:1888908]) HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0 Identity = 418/759 (55.07%), Postives = 557/759 (73.39%), Query Frame = 2
Query: 92 NRISPASV-HNKNFNT--------SNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
NR+ P+++ H ++++ S G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGVIS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + + +IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +A+ LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVD+V +AIDHI +GS HTD I+TEN+ TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRG+ H V DFS+ G + H++L
Sbjct: 30 NRVQPSAIRHVRSWSNIPFITVPLSRAHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGLGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIINHLADLLTDQREEILLANKKDLEEAEGRLASPLLKRLSLSTSKLNSLAIGLRQIAASSQESVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAIQLVNTREEVEDLCRLDKIIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLEVCIEVVDSVQEAIDHIHKYGSSHTDVIVTENEKTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGQDHVVSDFSEHGSLKYLHENL 785
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Match: Aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:MGI Symbol;Acc:MGI:1888908]) HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0 Identity = 418/761 (54.93%), Postives = 557/761 (73.19%), Query Frame = 2
Query: 92 NRISPASV-HNKNFNT--------SNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
NR+ P+++ H ++++ S G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGV IS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + + +IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +A+ LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVD+V +AIDHI +GS HTD I+TEN+ TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRG+ H V DFS+ G + H++L
Sbjct: 30 NRVQPSAIRHVRSWSNIPFITVPLSRAHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGLGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIINHLADLLTDQREEILLANKKDLEEAEGRLASPLLKRLSLSTSKLNSLAIGLRQIAASSQESVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAIQLVNTREEVEDLCRLDKIIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLEVCIEVVDSVQEAIDHIHKYGSSHTDVIVTENEKTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGQDHVVSDFSEHGSLKYLHENL 787
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Match: Aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:MGI Symbol;Acc:MGI:1888908]) HSP 1 Score: 139.813 bits (351), Expect = 1.117e-37 Identity = 75/144 (52.08%), Postives = 106/144 (73.61%), Query Frame = 2
Query: 92 NRISPASV-HNKNFNT--------SNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLY 490
NR+ P+++ H ++++ S G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LY
Sbjct: 30 NRVQPSAIRHVRSWSNIPFITVPLSRAHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEARACAAAGQSGLMALY 173
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Match: Aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:MGI Symbol;Acc:MGI:1888908]) HSP 1 Score: 110.153 bits (274), Expect = 3.124e-28 Identity = 49/73 (67.12%), Postives = 59/73 (80.82%), Query Frame = 2
Query: 1952 MKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLG 2170
++ EYG L +EVVD+V +AIDHI +GS HTD I+TEN+ TA FLQ+VDSACVF NASTRF+DGYRFGLG
Sbjct: 11 LRTEYGDLEVCIEVVDSVQEAIDHIHKYGSSHTDVIVTENEKTAEFFLQHVDSACVFWNASTRFSDGYRFGLG 83
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Match: Aldh18a1 (aldehyde dehydrogenase 18 family, member A1 [Source:MGI Symbol;Acc:MGI:1888908]) HSP 1 Score: 82.8037 bits (203), Expect = 6.422e-19 Identity = 42/83 (50.60%), Postives = 61/83 (73.49%), Query Frame = 2
Query: 311 LILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDF 553
++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF
Sbjct: 1 MMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDF 83
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Match: sp|Q5R4M8|P5CS_PONAB (Delta-1-pyrroline-5-carboxylate synthase OS=Pongo abelii OX=9601 GN=ALDH18A1 PE=2 SV=1) HSP 1 Score: 825.083 bits (2130), Expect = 0.000e+0 Identity = 417/733 (56.89%), Postives = 547/733 (74.62%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGV IS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + +++IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +AV LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVDNV DAIDHI +GS HTD I+TEN++TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRGK H V DFS+ G + H++L
Sbjct: 58 GKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAPPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTENENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENL 787
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Match: sp|Q9Z110|P5CS_MOUSE (Delta-1-pyrroline-5-carboxylate synthase OS=Mus musculus OX=10090 GN=Aldh18a1 PE=1 SV=2) HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0 Identity = 418/761 (54.93%), Postives = 557/761 (73.19%), Query Frame = 2
Query: 92 NRISPASV-HNKNFNT--------SNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
NR+ P+++ H ++++ S G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGV IS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + + +IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +A+ LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVD+V +AIDHI +GS HTD I+TEN+ TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRG+ H V DFS+ G + H++L
Sbjct: 30 NRVQPSAIRHVRSWSNIPFITVPLSRAHGKPFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGLGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIINHLADLLTDQREEILLANKKDLEEAEGRLASPLLKRLSLSTSKLNSLAIGLRQIAASSQESVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAIQLVNTREEVEDLCRLDKIIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLEVCIEVVDSVQEAIDHIHKYGSSHTDVIVTENEKTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGQDHVVSDFSEHGSLKYLHENL 787
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Match: sp|P54886|P5CS_HUMAN (Delta-1-pyrroline-5-carboxylate synthase OS=Homo sapiens OX=9606 GN=ALDH18A1 PE=1 SV=2) HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0 Identity = 416/733 (56.75%), Postives = 547/733 (74.62%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ+S + QG+E++LVTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + A+IL+T DF+++Q R NL GTL+ELL +NI+PI+NTNDAV PP + +SDLQGV IS+KDNDSLAAR+A EM DLLI++SDV+GL+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR L QRA I LADLL + +++IL+AN +D A+ L +L RL L++SKL +LAIGL QIA S + + RVLR+T +A +L LE++T+PIGVLLVIFESRPDCLPQVA+LAIASGNG+LLKGG EA +SN+ L L ++AL G+ +AV LV+ RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPV+GHSEG+CH++VD A+++ R+V+DSKC+YPAACNA+ETLLIH+ L+ T F+++ DML QV+I +GPK A L F +++ + ++ EYG L +EVVDNV DAIDHI +GS HTD I+TE+++TA FLQ+VDSACVF NASTRF+DGYRFGLGAEVGISTSRIHARGPVG++GLLT+KW+LRGK H V DFS+ G + H++L
Sbjct: 58 GKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGD-QQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAEGRLAAPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVK-EAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKS-LRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENL 787
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Match: sp|P54889|ALH13_CAEEL (Probable delta-1-pyrroline-5-carboxylate synthase OS=Caenorhabditis elegans OX=6239 GN=alh-13 PE=3 SV=2) HSP 1 Score: 800.816 bits (2067), Expect = 0.000e+0 Identity = 410/746 (54.96%), Postives = 536/746 (71.85%), Query Frame = 2
Query: 101 SPASVHNKNFNTSNILGR---VASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTP-QEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLN-SDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSL 2320
+P V N N+ S + + ++R+DLK ++R+VVKLGSAVITR DECGLALGRLASIVEQ+SE+ Q G+++++V+SGAVAFG+QKL + M+MS++QTL+ ++ D RACAA+GM GLMSLYEQLFQQYG+ A++LLTKPD +DQ R NL+ T+ LL LNIIPI+N NDAVAP P L+ + I DNDSLAAR++AE+ +LLII+S+V+G+Y+ PP+ EGS L+ TY + S + FG S+ GTGGMESK A AL G++ VI G ++A+ + V GKK+GT F T E +E AE R A +L ++ +R +R LA LL++ + I+ AN D AK + LD +L+RLK+T K+Q L GLN IADS + L+ RVL+K +++ L LE++T+PIG L+VIFESRPDCLPQVASLA+ASGN +LLKGG EA SNK L LV++AL + G DAV LV RED+ LLQL IDL+IPRGSS ++ ++ SK IPVLGH+EGVCHV++D+ + + A++IV+DSKCDYP+ACNA ET+LIHK L FF+ L M V++ +GPKLA LKF A M EYGSL CT+EVVDNV +A+ HI +GS HT++IITEN +TA FL++VDSAC FHNASTRFADGYRFGLGAEVGIST RIHARGPVGV+GLLT+KW+LRG+GH V+DF GK+S+ H++L
Sbjct: 50 TPVGVGNGNYCYSTKTRQKHPLINTRNDLKKAQRVVVKLGSAVITREDECGLALGRLASIVEQVSELQQSGRQMLIVSSGAVAFGRQKLRQELVMSMSMRQTLRGPSGMT-----ADKRACAASGMPGLMSLYEQLFQQYGITVAQVLLTKPDIDDDQRRKNLQATIESLLSLNIIPIVNANDAVAPDPKLN------MHISDNDSLAARLSAEIEAELLIILSNVNGVYTGPPDLEGSRLLYTYVPSENS-GVTFGANSKFGTGGMESKVTACVNALNNGVTTVITNGLAQDAITDAVAGKKIGTMFCNTKGYEGPPIEEVAEKCRDAGRQLAALSNKERGAMVRHLAALLVDKEKYIIEANQTDLANAKSAGLDPQLLNRLKMTPEKIQDLHAGLNTIADSAETLVGRVLKKVKISEGLFLEQVTVPIGSLMVIFESRPDCLPQVASLAMASGNALLLKGGKEAEESNKALHALVQEALGTHGFEMRDAVTLVRSREDVADLLQLKDLIDLIIPRGSSDLVRSMQEKSKGIPVLGHAEGVCHVYIDKDCDEQKAIQIVRDSKCDYPSACNAAETILIHKDLATAPFFDSLCSMFKAEGVKLHAGPKLAALLKFAPPP-AESMSFEYGSLECTLEVVDNVEEAVAHIIRYGSGHTESIITENTNTAEHFLKHVDSACAFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRGEGHLVEDFKNGKYSYLHENL 782
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Match: sp|O04226|P5CS1_ORYSJ (Delta-1-pyrroline-5-carboxylate synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=P5CS1 PE=2 SV=2) HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0 Identity = 331/731 (45.28%), Postives = 470/731 (64.30%), Query Frame = 2
Query: 146 LGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFF-----TLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSL 2320
+ V SRS ++D KR+++K+G+AV++R D LALGR+ ++ EQ+ E++ G E+ILVTSGAV G+Q+L ++ K + D+ + +D +ACAA G SGLM+LY+ LF Q + ++++L+T DF N + R L T+ LL L +IPI N NDA++ D G+ DNDSLA +A E+ DLLI++SDVDGLYS PP+E S +I TY + FG KSRVG GGM +K KAA A G VVI G ++L+++HG+K+GT F +D A AR S L +S +R + +AD L +++ I N+ D A+ + ++ +++RL + K+ +LA + +A+ D + N++L+KTE+A DL+LE+ + P+GVLL++FESRPD L Q+ASLAI SGNG+LLKGG EA SN L K++ A+ + + + LV+ R++I LL+L+ IDLVIPRGS++++++IK +K IPVLGH++G+CHV++D+ A+++ A IV D+K DYPAACNAMETLL+HK L+ + + + L V I+ GP K+L F A EY S++CT+E VD+V AIDHI +GS HTD I+T +D A TFL+ VDSA VFHNASTRF+DG RFGLGAEVGIST RIHARGPVGV+GLLT++WILRG+G V +THKSL
Sbjct: 1 MASVDPSRSFVRDVKRVIIKVGTAVVSRQD-GRLALGRVGALCEQVKELNSLGYEVILVTSGAVGVGRQRLRYR-------KLVNSSFADLQKPQMELDGKACAAVGQSGLMALYDMLFNQLDVSSSQLLVTDSDFENPKFREQLTETVESLLDLKVIPIFNENDAISTRKAPYEDSSGIFW--DNDSLAGLLALELKADLLILLSDVDGLYSGPPSEPSSKIIHTYIKEKHQQEITFGDKSRVGRGGMTAKVKAAVLASNSGTPVVITSGFENRSILKVLHGEKIGTLFHKNANLWESSKDVSTREMAVAARDCSRHLQNLSSEERKKILLDVADALEANEDLIRSENEADVAAAQVAGYEKPLVARLTIKPGKIASLAKSIRTLANMEDPI-NQILKKTEVADDLVLEKTSCPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEAIRSNTILHKVITDAI-PRNVGEKLIGLVTTRDEIADLLKLDDVIDLVIPRGSNKLVSQIKASTK-IPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAACNAMETLLVHKDLMKSPGLDDILVALKTEGVNIYGGPIAHKALGFPKA---VSFHHEYSSMACTVEFVDDVQSAIDHIHRYGSAHTDCIVTTDDKVAETFLRRVDSAAVFHNASTRFSDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGRGQVVN--GDKDVVYTHKSL 713
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Match: A0A267FVY1 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000463g3 PE=3 SV=1) HSP 1 Score: 909.827 bits (2350), Expect = 0.000e+0 Identity = 449/725 (61.93%), Postives = 557/725 (76.83%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLA 2326
SR DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QG+ +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSDLQGV+SI+DNDSLAAR+A E+N DLLIIMSDV GLY+ PP+ E S L+ T+ L+FG KSRVGTGGMESK KAA+WA+ +G SVVIC+GT EN + ++ GKKVGTFFTL P E VE QA AR AS +L ++ RA+ IRKLADLLIE Q DIL+AN++D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ E ALR+V DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F A ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSAC FHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF+ G F H++LL+
Sbjct: 53 SRQDLKYAEHVVVKLGSAVITRADECGLALGRLASIVEQISDLCLQGRHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLRLNIVPIVNTNDAVAPPPSQDSDLQGVLSIQDNDSLAARLAVEVNADLLIIMSDVKGLYTSPPDREDSRLLHTFNPDFDGARLQFGSKSRVGTGGMESKVKAAQWAVRKGTSVVICEGTEENVIPSVLEGKKVGTFFTLAPPEGVGVEQQAWEARCASHQLQAMPADVRASVIRKLADLLIEQQADILLANNQDLSSAA-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADKEKALRVVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPAVSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSFNTFLANVDSACAFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFADGTKKFVHENLLS 773
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Match: A0A1I8GG97 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 909.827 bits (2350), Expect = 0.000e+0 Identity = 449/725 (61.93%), Postives = 557/725 (76.83%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLA 2326
SR DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QG+ +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSDLQGV+SI+DNDSLAAR+A E+N DLLIIMSDV GLY+ PP+ E S L+ T+ L+FG KSRVGTGGMESK KAA+WA+ +G SVVIC+GT EN + ++ GKKVGTFFTL P E VE QA AR AS +L ++ RA+ IRKLADLLIE Q DIL+AN++D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ E ALR+V DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F A ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSAC FHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF+ G F H++LL+
Sbjct: 880 SRQDLKYAEHVVVKLGSAVITRADECGLALGRLASIVEQISDLCLQGRHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLRLNIVPIVNTNDAVAPPPSQDSDLQGVLSIQDNDSLAARLAVEVNADLLIIMSDVKGLYTSPPDREDSRLLHTFNPDFDGARLQFGSKSRVGTGGMESKVKAAQWAVRKGTSVVICEGTEENVIPSVLEGKKVGTFFTLAPPEGVGVEQQAWEARCASHQLQAMPADVRASVIRKLADLLIEQQADILLANNQDLSSAA-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADKEKALRVVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPAVSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSFNTFLANVDSACAFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFADGTKKFVHENLLS 1600 HSP 2 Score: 887.486 bits (2292), Expect = 0.000e+0 Identity = 447/758 (58.97%), Postives = 557/758 (73.48%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDV---------------------------------DGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLA 2326
+R DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QGK +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSD+QGV+ I+DNDSLAAR+A E+N +LLIIMSDV GLY+ PP+ E S L+ T+ S L+FG KS VGTGGMESK KAA+WA+ +G SVVIC+GT N + +++GKKVGTFFTL P E VE QA AR AS +L +N RA+ IRKLADLLIE Q DIL+AN D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ + A+RIV DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F AT ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSACVFHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF++G F H++LL+
Sbjct: 55 TRQDLKYAENVVVKLGSAVITRADECGLALGRLASIVEQISDMCLQGKHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLQLNIVPIVNTNDAVAPPPRHDSDMQGVLRIQDNDSLAARLAVEVNAELLIIMSDVKVSLGVQAEHGWSQIQILFFSLSFSPCRYPPIRQGLYTSPPDREDSRLLHTFNPDFDSAMLQFGSKSSVGTGGMESKVKAAQWAVRKGTSVVICEGTAANVIPSVLNGKKVGTFFTLAPPEGVGVEQQAWEARSASQQLQAMPANDRASVIRKLADLLIERQADILLANSLDLGNAT-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADRDRAIRIVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPATSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSVNTFLTNVDSACVFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFAEGTKKFVHENLLS 808
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Match: A0A267FTM0 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000463g2 PE=3 SV=1) HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0 Identity = 447/725 (61.66%), Postives = 557/725 (76.83%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLA 2326
+R DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QGK +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSD+QGV+ I+DNDSLAAR+A E+N +LLIIMSDV GLY+ PP+ E S L+ T+ S L+FG KS VGTGGMESK KAA+WA+ +G SVVIC+GT N + +++GKKVGTFFTL P E VE QA AR AS +L +N RA+ IRKLADLLIE Q DIL+AN D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ + A+RIV DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F AT ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSACVFHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF++G F H++LL+
Sbjct: 46 TRQDLKYAENVVVKLGSAVITRADECGLALGRLASIVEQISDMCLQGKHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLQLNIVPIVNTNDAVAPPPRHDSDMQGVLRIQDNDSLAARLAVEVNAELLIIMSDVKGLYTSPPDREDSRLLHTFNPDFDSAMLQFGSKSSVGTGGMESKVKAAQWAVRKGTSVVICEGTAANVIPSVLNGKKVGTFFTLAPPEGVGVEQQAWEARSASQQLQAMPANDRASVIRKLADLLIERQADILLANSLDLGNAT-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADRDRAIRIVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPATSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSVNTFLTNVDSACVFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFAEGTKKFVHENLLS 766
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Match: A0A1I8G6V3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 905.59 bits (2339), Expect = 0.000e+0 Identity = 449/724 (62.02%), Postives = 554/724 (76.52%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLL 2323
SR DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QG+ +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSDLQGV+SI+DNDSLAAR+A E+N DLLIIMSDV GLY+ PP+ E S L+ T+ L+FG KSRVGTGGMESK KAA+WA+ +G SVVIC+GT EN + ++ GKKVGTFFTL P E VE QA AR AS +L ++ RA+ IRKLADLLIE Q DIL+AN++D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ E ALR+V DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F A ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+ + +TFL NVDSAC FHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF+ G F H++LL
Sbjct: 895 SRQDLKYAEHVVVKLGSAVITRADECGLALGRLASIVEQISDLCLQGRHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLRLNIVPIVNTNDAVAPPPSQDSDLQGVLSIQDNDSLAARLAVEVNADLLIIMSDVKGLYTSPPDREDSRLLYTFNPDFDGARLQFGSKSRVGTGGMESKVKAAQWAVRKGTSVVICEGTEENVIPSVLEGKKVGTFFTLAPPEGVGVEQQAWEARCASHQLQAMPADVRASIIRKLADLLIEQQADILLANNQDLSSAA-DLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAQQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADKEKALRVVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPAVSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENEPSVNTFLANVDSACAFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFADGTKKFVHENLL 1614 HSP 2 Score: 884.789 bits (2285), Expect = 0.000e+0 Identity = 447/757 (59.05%), Postives = 555/757 (73.32%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDV---------------------------------DGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLL 2323
+R DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QGK +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSD+QGV+ I+DNDSLAAR+A E+N +LLIIMSDV GLY+ PP+ E S L+ T+ S L+FG KS VGTGGMESK KAA+WA+ +G SVVIC+GT N + +++GKKVGTFFTL P E VE QA AR AS +L +N RA+ IRKLADLLIE Q DIL+AN D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ + A+RIV DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F AT ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSACVFHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF+ G F H++LL
Sbjct: 55 TRQDLKYAENVVVKLGSAVITRADECGLALGRLASIVEQISDMCLQGKHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLQLNIVPIVNTNDAVAPPPRHDSDMQGVLRIQDNDSLAARLAVEVNAELLIIMSDVKVSLGVQAEHGWSQIQILFFSLSFSPCRYPPIRQGLYTSPPDREDSRLLHTFNPDFDSAMLQFGSKSSVGTGGMESKVKAAQWAVRKGTSVVICEGTAANVIPSVLNGKKVGTFFTLAPPEGVGVEQQAWEARSASQQLQAMPANDRASVIRKLADLLIERQADILLANSLDLGNAT-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADRDRAIRIVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPATSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSVNTFLTNVDSACVFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFADGTKKFVHENLL 807
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Match: A0A267FXW8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027999g2 PE=3 SV=1) HSP 1 Score: 904.434 bits (2336), Expect = 0.000e+0 Identity = 447/725 (61.66%), Postives = 556/725 (76.69%), Query Frame = 2
Query: 164 SRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISE----IHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLA 2326
+R DLK ++ +VVKLGSAVITR DECGLALGRLASIVEQIS++ QGK +LVTSGAVAFGKQKL Q M+MSI +TL D++ +DPRACAA GMSGLM+LYE LF QYG+QTAEILLTKPDF + +RANLR TL ELL LNI+PI+NTNDAVAPPP DSD+QGV+ I+DNDSLAAR+A E+N +LLIIMSDV GLY+ PP+ E S L+ T+ S L+FG KS VGTGGMESK KAA+WA+ +G SVVIC+GT N + +++GKKVGTFFTL P E VE QA AR AS +L +N RA+ IRKLADLLIE Q DIL+AN D A L ++L+RLKLT KLQ LA GL QIADSC LL RVLR+T+LA L LE++T+PIGVLLVIFESRPDCLPQVA+L+IASGNG+LLKGGSEA+ +N L +LV++A+ + GL+ V LV RE + LL + +++DLVIPRGS+QMI I+ S IPVLGHS+GVCHV++DE A+ + A+RIV DSKCDYPAACNAMET+L+H+ + + FF++L+D L V++ +GP+LAK ++F AT ++ EY SL C +E+V V +A+DHI +GS HTD+I++EN+++ +TFL NVDSACVFHN STRF+DGYRFGLGAEVGISTSRIHARGPVGV+GLLT+KW+L G G V DF+ G F H++LL+
Sbjct: 47 TRQDLKYAENVVVKLGSAVITRADECGLALGRLASIVEQISDMCLQGKHCVLVTSGAVAFGKQKLRQQQMMSMSIGETLSA--DVNSRRKAFQSQIDPRACAATGMSGLMALYEALFMQYGIQTAEILLTKPDFEDANSRANLRSTLAELLQLNIVPIVNTNDAVAPPPRHDSDMQGVLRIQDNDSLAARLAVEVNAELLIIMSDVKGLYTSPPDREDSRLLHTFNPDFDSAMLQFGSKSSVGTGGMESKVKAAQWAVRKGTSVVICEGTAANVIPSVLNGKKVGTFFTLAPPEGVGVEQQAWEARSASQQLQAMPANDRASVIRKLADLLIERQADILLANSLDLGNAT-GLSPHLLARLKLTPEKLQVLADGLRQIADSCPGLLGRVLRRTKLAEGLKLEQVTVPIGVLLVIFESRPDCLPQVAALSIASGNGLLLKGGSEAHQTNGALHRLVEEAVRAHGLSPRLVGLVGGREQVSDLLAMGEHVDLVIPRGSAQMIRDIQRQSVGIPVLGHSDGVCHVYIDELADRDRAIRIVLDSKCDYPAACNAMETVLLHESHLKSGFFDELFDALRSRDVKVNTGPRLAKKIRF-GPSPATSLRTEYSSLECCIEIVSGVAEAVDHINTYGSHHTDSIVSENETSVNTFLTNVDSACVFHNCSTRFSDGYRFGLGAEVGISTSRIHARGPVGVEGLLTTKWLLHGSGDTVSDFADGTKKFVHENLLS 767
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Cavefish
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:103029980]) HSP 1 Score: 808.52 bits (2087), Expect = 0.000e+0 Identity = 423/751 (56.32%), Postives = 547/751 (72.84%), Query Frame = 2
Query: 107 ASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT----LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
A H K+F + RS+L+ +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + L D+S P ++ RACAAAG SGLM+LYE +F QY TA+IL+T DF++DQ R NL TL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DLLI +SDV+GLY PP + + L+DT+ ++ +G KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR A L QR+ I +ADLL E + +IL AN +D LA S + ML RL L+SSKL +LAIGL QIA S + + RVLR+T +A +L LE+IT+PIGVLLVIFESRPDCLPQV++LAIASGN +LLKGG EA N+N+ L ++ ++AL G+ DA+ LVS RE+++ L +L K IDL+IPRGSSQ++ I+ +K IPVLGHSEG+CHV+VD A+++ ++IV+DSKCDYPAACNAMETLL+H+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD++ +A+DHI +GS HTD IITEN+ TA FLQ VDSACVF NAS+RFADGYRFGLGAEVGIST+RIHARGPVG++GLLT+KW+LRG G DFS QG F H++L
Sbjct: 32 ARAHGKSF----------AHRSELRQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMS--LPVLEARACAAAGQSGLMALYEAMFTQYSTCTAQILVTNLDFHDDQKRRNLNSTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMRVDLLIALSDVEGLYDSPPGTDDAKLLDTFYPGD-QQSITYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTHPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARTAGRTLASLLPEQRSEIIYAMADLLTEKREEILSANKKDMELALNSGRMPAVMLKRLSLSSSKLNSLAIGLRQIAVSSQDSVGRVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVSALAIASGNALLLKGGKEAANTNRILHEVAQEALSMHGVK-DAIQLVSTREEVEDLCRLEKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGICHVYVDHEASIDKVIKIVRDSKCDYPAACNAMETLLVHRDVLRTPLFDQIIDMLRTEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCIEVVDSMQEAVDHIHKYGSSHTDVIITENEETAEQFLQLVDSACVFWNASSRFADGYRFGLGAEVGISTARIHARGPVGLEGLLTTKWVLRGDGQTAADFSEQGSMKFLHENL 767
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Cavefish
Match: aldh18a1 (aldehyde dehydrogenase 18 family member A1 [Source:NCBI gene;Acc:103029980]) HSP 1 Score: 736.487 bits (1900), Expect = 0.000e+0 Identity = 386/686 (56.27%), Postives = 503/686 (73.32%), Query Frame = 2
Query: 149 GRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT----LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLG 2170
G+ + RS+L+ +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + L D+S P ++ RACAAAG SGLM+LYE +F QY TA+IL+T DF++DQ R NL TL+ELL +NI+PIINTNDAV PPP+ +SDLQGV ISIKDNDSLAAR+A EM DLLI +SDV+GLY PP + + L+DT+ ++ +G KSRVG GGME+K KAA WAL G SVVI GTH + + +IV GKKVGTFF+ VE Q E+AR A L QR+ I +ADLL E + +IL AN +D LA S + ML RL L+SSKL +LAIGL QIA S + + RVLR+T +A +L LE+IT+PIGVLLVIFESRPDCLPQV++LAIASGN +LLKGG EA N+N+ L ++ ++AL G+ DA+ LVS RE+++ L +L K IDL+IPRGSSQ++ I+ +K IPVLGHSEG+CHV+VD A+++ ++IV+DSKCDYPAACNAMETLL+H+ ++ T F+++ DML QV+I +GP+ A L F +++ + ++ EYG L C +EVVD++ +A+DHI +GS HTD IITEN+ TA FLQ VDSACVF NAS+RFADGYRFGLG
Sbjct: 36 GKSFAHRSELRQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKDMS--LPVLEARACAAAGQSGLMALYEAMFTQYSTCTAQILVTNLDFHDDQKRRNLNSTLHELLRMNIVPIINTNDAVVPPPEPNSDLQGVNVISIKDNDSLAARLAVEMRVDLLIALSDVEGLYDSPPGTDDAKLLDTFYPGD-QQSITYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTHPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARTAGRTLASLLPEQRSEIIYAMADLLTEKREEILSANKKDMELALNSGRMPAVMLKRLSLSSSKLNSLAIGLRQIAVSSQDSVGRVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVSALAIASGNALLLKGGKEAANTNRILHEVAQEALSMHGVK-DAIQLVSTREEVEDLCRLEKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGICHVYVDHEASIDKVIKIVRDSKCDYPAACNAMETLLVHRDVLRTPLFDQIIDMLRTEQVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCIEVVDSMQEAVDHIHKYGSSHTDVIITENEETAEQFLQLVDSACVFWNASSRFADGYRFGLG 716
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Yeast
Match: PRO2 (Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis [Source:SGD;Acc:S000005850]) HSP 1 Score: 305.064 bits (780), Expect = 6.931e-96 Identity = 177/447 (39.60%), Postives = 270/447 (60.40%), Query Frame = 2
Query: 1055 AELARKASLELFQSTSNQ-RANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKL-TSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQAL----ESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKL----------------AKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDF--SQGKFSFTHKSL 2320
A+ ARKA + ++ SN+ R++ + K+ D L + + I AN D +AK + L +++L RL L K + + G+ +A+ ++ + +V EL L L ++T P+GVLLVIFESRP+ + + +L+I SGN +LKGG E+ N+ + + K+V + G+ +V L+ R+D+ LL ++YIDLV+PRGS+ ++ KIK +K IPVLGH++G+C +++DE A+L A RI D+K +YPA CNAMETLLI+ K ++++E L ++ + V I + L A K +A KE+ SL + V + AI HI S+HTD I+TEN + A F++ VDS+ V+ NASTRFADG+R+G GAEVGISTS+IHARGPVG+DGL++ ++ +RG G D+ + G +F HK L
Sbjct: 8 AKNARKAG-NILKTISNEGRSDILYKIHDALKANAHAIEEANKIDLAVAKETGLADSLLKRLDLFKGDKFEVMLQGIKDVAE-LEDPVGKVKMARELDDGLTLYQVTAPVGVLLVIFESRPEVIANITALSIKSGNAAILKGGKESVNTFREMAKIVNDTIAQFQSETGVPVGSVQLIETRQDVSDLLDQDEYIDLVVPRGSNALVRKIKDTTK-IPVLGHADGICSIYLDEDADLIKAKRISLDAKTNYPAGCNAMETLLINPKF--SKWWEVLENLTLEGGVTIHATKDLKTAYFDKLNELGKLTEAIQCKTVDADEEQDFDKEFLSLDLAAKFVTSTESAIQHINTHSSRHTDAIVTENKANAEKFMKGVDSSGVYWNASTRFADGFRYGFGAEVGISTSKIHARGPVGLDGLVSYQYQIRGDGQVASDYLGAGGNKAFVHKDL 449
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Yeast
Match: YHR033W_mRNA (Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication [Source:SGD;Acc:S000001075]) HSP 1 Score: 123.25 bits (308), Expect = 1.794e-30 Identity = 88/269 (32.71%), Postives = 132/269 (49.07%), Query Frame = 2
Query: 194 IVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYS-RP---PNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIV 988
IV+KLGS+ + L + IVE ++ + + G ++I+V+SG +A G L +I K L ++ +A AA G L++ + LF QYG QTA+ILLT+ D N R T+NELL + +IPI+N ND ++ I DND+L+A AA + D L +++DVD LY+ P P+ VL+ + G S VGTGGM +K AA+ A GI ++ K + +IV
Sbjct: 7 IVIKLGSSSLVDESTKEPKLSIMTLIVETVTNLKRMGHKVIIVSSGGIAVGLDAL--------NIPHKPKQLSEV---------QAIAAVGQGRLIARWNMLFSQYGEQTAQILLTRNDILRWNQYNNARNTINELLAMGVIPIVNENDTLS---------ISEIEFGDNDTLSAITAALVGADFLFLLTDVDCLYTDNPRTNPDARPIVLVPELSEGLPGVNTSSGSGSEVGTGGMRTKLIAADLASNAGIETIVMKSDRPEYVPKIV 249
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Yeast
Match: PRO1 (Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication [Source:SGD;Acc:S000002708]) HSP 1 Score: 112.849 bits (281), Expect = 5.877e-27 Identity = 85/268 (31.72%), Postives = 137/268 (51.12%), Query Frame = 2
Query: 185 SKRIVVKLGSA--VITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALE---------FGGKSRVGTGGMESKFKAAEWALTQGISVVICK 955
S IV+KLGS+ V + E LA+ ++ IVE + ++ + G ++I+V+SG +A G + TM + + K L ++ +A AA G L+ ++ LF Q+ + A+ILLT+ D + N + T+NELL++ +IPI+N ND ++ I DND+L+A +A ++ D L +++DVD LY+ P + +V P L G S VGTGGME+K AA+ A G+ +I K
Sbjct: 9 SYTIVIKLGSSSLVDEKTKEPKLAI--MSLIVETVVKLRRMGHKVIIVSSGGIAVGLR--------TMRMNKRPKHLAEV---------QAIAAIGQGRLIGRWDLLFSQFDQRIAQILLTRNDILDWTQYKNAQNTINELLNMGVIPIVNENDTLSVRE---------IKFGDNDTLSAITSALIHADYLFLLTDVDCLYTDNPRTNPDAM-----PILVVPDLSKGLPGVNTAGGSGSDVGTGGMETKLVAADLATNAGVHTLIMK 243
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Nematostella
Match: EDO38923 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBH3]) HSP 1 Score: 801.971 bits (2070), Expect = 0.000e+0 Identity = 414/748 (55.35%), Postives = 533/748 (71.26%), Query Frame = 2
Query: 134 TSNILGRVASSRSDLKDS-----------KRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKT---LHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHEN--ALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQ-GKFSFTHKSL 2320
+S + R SS D KDS KR++VKLGSAVITR DECG+ALGRL+SIVEQ++E+ G+E++LVTSGAVAFGKQKL H+ ++ S++QTLK L+ + ++ + PRACAA G GLMSLYE +F QYGL +ILLT+PD + R NLR T+ L+ + IPIIN NDAVAPPP LD DL GVIS+KDNDSLAA +A E+ DLLI++SDV+G+Y PP+ S ++ T+ + FG KSRVG GGMESK ++A WAL +G SVVI GT E+ + +I++G+ VGTFFT Q +E QA AR L ++ QR I KLADLLIE + DIL AN D A+ L M++RL LT SKL+ LA GL QIAD + RVLRKT +A DL+L++ T+PIGVL+VIFESRPD L QVASLAI++ NG+LLKGG EAY+SNKCL LV++ALE + +AV+L+S R+ + LL+L IDLVIPRGS +MI +I+ SK IPVLGHS+G+CHV++D++A+ E A+ IV DSKCDYPAACN+METLL+H+ L+GT +K+ L + V+I++GP+L K L A+ M EYG L C +E+V+NV DAI HI FGS HT++IIT +D TA+ FL++VDSACVFHNASTRFADGYRFGLGAEVGIST RIHARGPVGV+GLLT+KW LRG+GH V DF+ G +++ H++L
Sbjct: 3 SSRVAARSLSSGYDSKDSVYHRAELKKKAKRVIVKLGSAVITRGDECGVALGRLSSIVEQLAELQNSGREMLLVTSGAVAFGKQKLRHENLLSQSVRQTLKPQDGLNKVGKLKSY--PRACAAVGQGGLMSLYEAMFAQYGLTCGQILLTRPDLRDAYARENLRSTVEALISMKCIPIINENDAVAPPPGLDQDLAGVISLKDNDSLAALLAVEIRADLLILLSDVNGIYDGPPDNPQSQIMHTFHPDNFKD-VTFGSKSRVGRGGMESKVRSAVWALERGTSVVIANGTGEDYHVVRDIINGRNVGTFFTTAEQIGPSIEDQATKARDGGRALQALSAEQRKQIIYKLADLLIERKTDILDANTMDLEAARQEGKLPAPMMARLLLTPSKLEQLAQGLRQIADGSHKNIGRVLRKTRVAKDLVLQQETVPIGVLMVIFESRPDALVQVASLAISTANGLLLKGGKEAYHSNKCLHSLVQEALELHA-SPEAVSLISTRDQVGDLLKLEGLIDLVIPRGSPEMIRQIQQDSKGIPVLGHSDGICHVYMDKYADFEMAVDIVVDSKCDYPAACNSMETLLVHRDLLGTSALDKVLKALRERGVKIYAGPRLVKELPL-GPLPASSMSIEYGGLECAVEIVENVEDAIAHIHKFGSSHTESIITNDDETANKFLKSVDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWTLRGEGHTVADFADGGTYTYLHEAL 745
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Medaka
Match: ALDH18A1 (delta-1-pyrroline-5-carboxylate synthase [Source:NCBI gene;Acc:101173087]) HSP 1 Score: 820.846 bits (2119), Expect = 0.000e+0 Identity = 419/743 (56.39%), Postives = 547/743 (73.62%), Query Frame = 2
Query: 125 NFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHD-ISEIH-PHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS--LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSL 2320
F+ G+ + RS+LK +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + QG+E+++VTSGAVAFGKQ+L H+ ++ S++Q L + + + E+ P ++ RACAAAG SGLM+LYE +F QY + TA+IL+T DF+++Q R NL TL+ELL +NI+PIINTNDAV PPP +SDLQGV ISIKDNDSLAAR+A EM DLLI +SDV GLY PP + + LID + ++ +G KSRVG GGME+K KAA WAL G SVVI GT + + +IV GKKVGTFF+ VE Q E+AR A L QR I LA+LL E + +IL AN +D LA + L + +++RL L++SKL +LAIGL Q+A + + + RVLR+T +A +L LE+IT+PIGVLLVIFESRPDCLPQV++LAIASGN +LLKGG EA N+NK L +L ++AL G+ SDA+ LVS RE+++ L +L+K IDL+IPRGSSQ++ I+ +K IPVLGHSEGVCHV++D A+++ AL IV+DSKCDYPAACNAMETLLIH+ L+ T F+++ DML Q V+I +GP+ A L F +++ + ++ EYG L C +EVVD + DA+DHI +GS HTD I+TEN+ TA FLQ VDSACVF NAS+RFADGYRFGLGAEVGIST+RIHARGPVG++GLLT+KW+LRG+G V DFS QG + H+++
Sbjct: 35 KFSLPRPHGKSFAHRSELKQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMSVPVLEARACAAAGQSGLMALYEAMFTQYSICTAQILVTNLDFHDEQKRRNLNSTLHELLRMNIVPIINTNDAVVPPPVPNSDLQGVNVISIKDNDSLAARLAVEMKADLLIALSDVQGLYDSPPGTDDAKLIDIFYPGD-QQSVTYGTKSRVGIGGMEAKVKAALWALQGGTSVVIANGTDPKVTGHVITDIVEGKKVGTFFSEVKPAGPTVEQQTEMARHAGRTLASLLPEQREEIICCLAELLTERKEEILTANRKDMELASATGFLPQPLINRLSLSTSKLNSLAIGLRQLAVASRDSVGRVLRRTRVANNLELEQITVPIGVLLVIFESRPDCLPQVSALAIASGNALLLKGGKEASNTNKILHQLTQEALSIHGV-SDAIQLVSTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQRAAKGIPVLGHSEGVCHVYIDADASIDKALDIVKDSKCDYPAACNAMETLLIHRDLLRTPMFDQIIDMLRQEHVKIHAGPRFASYLTFSPSEVKS-LRTEYGDLECCIEVVDGMQDAVDHIHKYGSSHTDVIVTENEETAEQFLQQVDSACVFWNASSRFADGYRFGLGAEVGISTARIHARGPVGLEGLLTTKWVLRGEGQTVSDFSEQGSMKYLHENI 774
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Medaka
Match: aldh18a1 (delta-1-pyrroline-5-carboxylate synthase [Source:NCBI gene;Acc:101169240]) HSP 1 Score: 789.26 bits (2037), Expect = 0.000e+0 Identity = 408/729 (55.97%), Postives = 532/729 (72.98%), Query Frame = 2
Query: 176 LKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLH----DISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGV--ISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHE----NALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFS-QGKFSFTHKSLLAE 2329
L+ +KRIVVKLGSAV+TR DECGLALGRLASIVEQ++ + G+E+++VTSGAVAFGKQ+L H+ ++ S++Q L++ H D+S P ++ RACAAAG SGLM+LYE +F QY TA++L+T DF++D+ R NL TL ELL +NI+PIINTNDAV PPP+ +SDL GV ISIKDNDSLAAR+A EM DLLI +SDV+GLY+ PP + + LID + ++ FG KSRVG GGME+K KAA WAL G SVVI GT+ + + +IV GKKVGTFF+ T VE Q E+AR + L + +R+ I +LADLL E +IL AN D +A L ML RL L+ +K+ +L+IGL QIA S N RVLR+T +A +L LE+IT+PIGV+LVIFE+RPDCLPQV++LAIASGN +LLKGG EA N+N L +L ++AL GL +AV +VS RE+++ L +L+K IDL+IPRGS Q++ IK +K IPVLGHSEGVCHV+VD A+ + + IV+DSKC+YPAACNAMETLLIH+ ++ T F+++ DML +V+I +GP+ A L F ++ A ++ EY L C +EVVD++ +A++HI +GS HTD IITEN TA FLQ +DSACVF NAS+RFADGYRFGLGAEVGIST+RIHARGPVG++GLLT+KW+LRG GH V DFS QG ++ H++L E
Sbjct: 1 LRQAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVAMLQNHGREMMIVTSGAVAFGKQRLRHEILLSQSVRQALQSGHNQPKDMS--LPALEARACAAAGQSGLMALYEAMFSQYSTCTAQVLVTNLDFHDDEKRQNLHSTLQELLRMNIVPIINTNDAVVPPPEPNSDLHGVNVISIKDNDSLAARLAVEMKADLLIALSDVEGLYNSPPGTDDAKLIDIFYPGD-QHSITFGTKSRVGLGGMEAKVKAALWALQGGTSVVIANGTNPKVTGHVITDIVEGKKVGTFFSETKPAGPSVEEQTEMARSSGRILASLQAEKRSEIICRLADLLTEKTEEILAANKLDLEVASSRLPSAMLKRLSLSVAKINSLSIGLRQIATSAQNSAGRVLRRTRVAHNLELEQITVPIGVVLVIFEARPDCLPQVSALAIASGNALLLKGGKEAANTNHVLLQLTQEALSMHGLR-EAVQMVSTREEVEGLCRLDKMIDLIIPRGSFQLVQDIKKAAKGIPVLGHSEGVCHVYVDADASADKVIGIVRDSKCEYPAACNAMETLLIHRDILRTPLFDQIIDMLRTERVRIHAGPRFASYLTFSPSE-AKSLRTEYNDLECCVEVVDSMQEAVEHIHKYGSSHTDVIITENAKTAEQFLQQLDSACVFWNASSRFADGYRFGLGAEVGISTARIHARGPVGIEGLLTTKWVLRGDGHTVADFSEQGTQTYLHENLPLE 724
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Planmine SMEST
Match: SMESG000045474.1 (SMESG000045474.1) HSP 1 Score: 1551.18 bits (4015), Expect = 0.000e+0 Identity = 754/754 (100.00%), Postives = 754/754 (100.00%), Query Frame = 2
Query: 68 MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE 2329
MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE
Sbjct: 1 MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE 754
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Planmine SMEST
Match: SMESG000045474.1 (SMESG000045474.1) HSP 1 Score: 1546.56 bits (4003), Expect = 0.000e+0 Identity = 754/755 (99.87%), Postives = 754/755 (99.87%), Query Frame = 2
Query: 68 MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS-LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE 2329
MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYS LDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE
Sbjct: 1 MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAVITRVDECGLALGRLASIVEQISEISQQGKELILVTSGAVAFGKQKLLHQAKMTMSIKQTLKTLHDISEIHPHVDPRACAAAGMSGLMSLYEQLFQQYGLQTAEILLTKPDFYNDQTRANLRGTLNELLHLNIIPIINTNDAVAPPPDLDSDLQGVISIKDNDSLAARVAAEMNCDLLIIMSDVDGLYSRPPNEEGSVLIDTYCTTMVSPALEFGGKSRVGTGGMESKFKAAEWALTQGISVVICKGTHENALLEIVHGKKVGTFFTLTPQEDNQVELQAELARKASLELFQSTSNQRANSIRKLADLLIEHQNDILIANDEDFRLAKYSGLDENMLSRLKLTSSKLQTLAIGLNQIADSCDNLLNRVLRKTELAPDLILEEITIPIGVLLVIFESRPDCLPQVASLAIASGNGVLLKGGSEAYNSNKCLFKLVKQALESQGLNSDAVNLVSKREDIQSLLQLNKYIDLVIPRGSSQMINKIKCMSKSIPVLGHSEGVCHVFVDEHANLETALRIVQDSKCDYPAACNAMETLLIHKKLIGTEFFEKLYDMLGQNQVQIFSGPKLAKSLKFYNAQLATQMKKEYGSLSCTMEVVDNVNDAIDHIRIFGSQHTDTIITENDSTASTFLQNVDSACVFHNASTRFADGYRFGLGAEVGISTSRIHARGPVGVDGLLTSKWILRGKGHCVQDFSQGKFSFTHKSLLAE 755 The following BLAST results are available for this feature:
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of Delta-1-pyrroline-5-carboxylate synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005571 ID=SMED30005571|Name=Delta-1-pyrroline-5-carboxylate synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=2389bpback to top protein sequence of SMED30005571-orf-1 >SMED30005571-orf-1 ID=SMED30005571-orf-1|Name=SMED30005571-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=755bp MLKISRFLNRISPASVHNKNFNTSNILGRVASSRSDLKDSKRIVVKLGSAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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