SMED30005565

Overview
NameSMED30005565
Smed IDSMED30005565
Length (bp)340
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30005565

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 21

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
non-ciliated neuronSMED30005565h1SMcG0020555 dd_Smed_v4_8271_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30005565h1SMcG0020555 dd_Smed_v4_7137_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0016241 Contig4650newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0016241 Contig4650uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0020555 Contig4650newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0020555 Contig4650uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0020555 Contig12277newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005565h1SMcG0020555 Contig12277uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
ovarySMED30005565h1SMcG0016193 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005565h1SMcG0016193 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0016193 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0016193 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005565h1SMcG0016196 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005565h1SMcG0016196 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0016196 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0016196 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005565h1SMcG0020555 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
ovarySMED30005565h1SMcG0020555 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0020555 Contig4649uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30005565h1SMcG0020555 Contig4649newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Category 3 cellSMED30005565h1SMcG0020555 dd_Smed_v4_8271_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005565 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001385:39575..40032 +1471
v31.001385:39990..40430 -514
v31.001385:39787..40318 +507
Homology
BLAST of SMED30005565 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 62.7734 bits (151), Expect = 3.271e-12
Identity = 33/108 (30.56%), Postives = 54/108 (50.00%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR   I
Sbjct:  203 LLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPI 310          

HSP 2 Score: 50.8322 bits (120), Expect = 4.968e-8
Identity = 25/109 (22.94%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 3 Score: 50.0618 bits (118), Expect = 8.809e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 277          

HSP 4 Score: 49.6766 bits (117), Expect = 1.156e-7
Identity = 28/111 (25.23%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  N       G+ P+H A      +++E+L     +I
Sbjct:  299 VVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 409          

HSP 5 Score: 45.8246 bits (107), Expect = 2.581e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  N
Sbjct:  265 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAN 375          
BLAST of SMED30005565 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 62.7734 bits (151), Expect = 3.437e-12
Identity = 33/108 (30.56%), Postives = 54/108 (50.00%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR   I
Sbjct:  214 LLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPI 321          

HSP 2 Score: 55.4546 bits (132), Expect = 1.364e-9
Identity = 25/110 (22.73%), Postives = 52/110 (47.27%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+ P+H A    ++ I+ +L   N +
Sbjct:  673 MVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 782          

HSP 3 Score: 53.9138 bits (128), Expect = 5.578e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  607 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 717          

HSP 4 Score: 50.8322 bits (120), Expect = 5.254e-8
Identity = 25/109 (22.94%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N
Sbjct:  243 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 351          

HSP 5 Score: 50.0618 bits (118), Expect = 9.406e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  182 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 288          

HSP 6 Score: 50.0618 bits (118), Expect = 1.153e-7
Identity = 26/106 (24.53%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ NLLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  541 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLE 646          

HSP 7 Score: 50.0618 bits (118), Expect = 1.187e-7
Identity = 28/111 (25.23%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  N       G+ P+H A      +++E+L     +I
Sbjct:  310 VVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 420          

HSP 8 Score: 46.2098 bits (108), Expect = 2.335e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  640 IATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 750          

HSP 9 Score: 46.2098 bits (108), Expect = 2.727e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  N
Sbjct:  276 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAN 386          

HSP 10 Score: 45.8246 bits (107), Expect = 3.184e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H +  + + ++   LL+    +     + + P+H A      E+ NLLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  508 IVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 614          

HSP 11 Score: 45.0542 bits (105), Expect = 7.191e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  572 LEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANV 684          
BLAST of SMED30005565 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 62.7734 bits (151), Expect = 3.438e-12
Identity = 33/108 (30.56%), Postives = 54/108 (50.00%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR   I
Sbjct:  203 LLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPI 310          

HSP 2 Score: 55.4546 bits (132), Expect = 1.364e-9
Identity = 25/110 (22.73%), Postives = 52/110 (47.27%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+ P+H A    ++ I+ +L   N +
Sbjct:  662 MVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 771          

HSP 3 Score: 53.9138 bits (128), Expect = 5.578e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  596 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 706          

HSP 4 Score: 50.8322 bits (120), Expect = 5.254e-8
Identity = 25/109 (22.94%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 5 Score: 50.0618 bits (118), Expect = 9.407e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 277          

HSP 6 Score: 50.0618 bits (118), Expect = 1.153e-7
Identity = 26/106 (24.53%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ NLLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  530 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLE 635          

HSP 7 Score: 50.0618 bits (118), Expect = 1.187e-7
Identity = 28/111 (25.23%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  N       G+ P+H A      +++E+L     +I
Sbjct:  299 VVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 409          

HSP 8 Score: 46.2098 bits (108), Expect = 2.335e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  629 IATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 739          

HSP 9 Score: 46.2098 bits (108), Expect = 2.727e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  N
Sbjct:  265 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAN 375          

HSP 10 Score: 45.8246 bits (107), Expect = 3.185e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H +  + + ++   LL+    +     + + P+H A      E+ NLLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  497 IVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 603          

HSP 11 Score: 45.0542 bits (105), Expect = 7.191e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  561 LEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANV 673          
BLAST of SMED30005565 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 62.7734 bits (151), Expect = 3.500e-12
Identity = 33/108 (30.56%), Postives = 54/108 (50.00%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR   I
Sbjct:  220 LLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPI 327          

HSP 2 Score: 55.4546 bits (132), Expect = 1.389e-9
Identity = 25/110 (22.73%), Postives = 52/110 (47.27%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+ P+H A    ++ I+ +L   N +
Sbjct:  679 MVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 788          

HSP 3 Score: 53.9138 bits (128), Expect = 5.679e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  613 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 723          

HSP 4 Score: 50.8322 bits (120), Expect = 5.349e-8
Identity = 25/109 (22.94%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N
Sbjct:  249 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 357          

HSP 5 Score: 50.0618 bits (118), Expect = 9.577e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  188 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 294          

HSP 6 Score: 50.0618 bits (118), Expect = 1.174e-7
Identity = 26/106 (24.53%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ NLLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  547 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLE 652          

HSP 7 Score: 50.0618 bits (118), Expect = 1.209e-7
Identity = 28/111 (25.23%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  N       G+ P+H A      +++E+L     +I
Sbjct:  316 VVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 426          

HSP 8 Score: 46.2098 bits (108), Expect = 2.377e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  646 IATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 756          

HSP 9 Score: 46.2098 bits (108), Expect = 2.776e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  N
Sbjct:  282 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAN 392          

HSP 10 Score: 45.8246 bits (107), Expect = 3.242e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H +  + + ++   LL+    +     + + P+H A      E+ NLLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  514 IVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 620          

HSP 11 Score: 45.0542 bits (105), Expect = 7.320e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  578 LEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANV 690          
BLAST of SMED30005565 vs. Ensembl Human
Match: ANKK1 (ankyrin repeat and kinase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:21027])

HSP 1 Score: 62.7734 bits (151), Expect = 4.102e-12
Identity = 29/109 (26.61%), Postives = 49/109 (44.95%), Query Frame = -2
Query:    4 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 330
            V+LL+ H  +++C+T     P+  A   +  ++  LLL    D    D   W P+H+A    +     LLLD    ++  +  GW P+H A  N    +  +L  R  +
Sbjct:  378 VRLLLAHEVDVDCQTASGYTPLLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLLDHGACVDAQEREGWTPLHLAAQNNFENVARLLVSRQAD 486          

HSP 2 Score: 53.9138 bits (128), Expect = 4.217e-9
Identity = 28/108 (25.93%), Postives = 48/108 (44.44%), Query Frame = -2
Query:   16 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 339
              I+ LL E + N+         P+H A       + + LL+   D    +   W P+H A+ +     V  LL+ + N+   +  GW P H A +  N+ IL++L +
Sbjct:  606 LEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQCGADPNAAEQSGWTPLHLAVQRSTFLSVINLLEHHANVHARNKVGWTPAHLAALKGNTAILKVLVE 713          

HSP 3 Score: 53.9138 bits (128), Expect = 4.384e-9
Identity = 26/95 (27.37%), Postives = 46/95 (48.42%), Query Frame = -2
Query:   55 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAI 339
            + I ++L+ +  ++E  T Q   P+H A  K + EI +LL E + ++       W P+H A       +V+ LL    +    +  GW P+H A+
Sbjct:  573 YLICKMLLRYGASLELPTHQGWTPLHLAAYKGHLEIIHLLAESHANMGALGAVNWTPLHLAARHGEEAVVSALLQCGADPNAAEQSGWTPLHLAV 667          

HSP 4 Score: 52.373 bits (124), Expect = 1.845e-8
Identity = 25/93 (26.88%), Postives = 44/93 (47.31%), Query Frame = -2
Query:   16 CKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 294
            C  + K+ P+HF + + + E   LLL    D++C+    + P+  A   +  ++  LLL    +    D  GW P+H+A  N +     +L D
Sbjct:  357 CIYENKVTPLHFLVAQGSVEQVRLLLAHEVDVDCQTASGYTPLLIAAQDQQPDLCALLLAHGADANRVDEDGWAPLHFAAQNGDDGTARLLLD 449          

HSP 5 Score: 47.7506 bits (112), Expect = 6.018e-7
Identity = 25/112 (22.32%), Postives = 52/112 (46.43%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            S+V+LL      ++ + +    P+H A+ +        LL+     +  D   + P+H A  +    +  +LL    ++E   ++GW P+H A    + EI+ +L + + N+
Sbjct:  508 SLVKLLTSQGAELDAQQRNLRTPLHLAVERGKVRAIQHLLKSGAVPDALDQSGYGPLHTAAARGKYLICKMLLRYGASLELPTHQGWTPLHLAAYKGHLEIIHLLAESHANM 619          
BLAST of SMED30005565 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 57.7658 bits (138), Expect = 8.534e-11
Identity = 30/105 (28.57%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  +L++HN +    TK+   P+H A    N E+  LLLER   ++ E   +  P+H A +  N ++  LLL+   + +     G+ P+H A      EI   L 
Sbjct:  539 VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 2 Score: 55.0694 bits (131), Expect = 7.590e-10
Identity = 27/107 (25.23%), Postives = 54/107 (50.47%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V++LI +   ++ + ++   P+H A    N++I  LLL+   +        + P+H A  +   E+  +LLD N +      +G+ P+H A    N E++ +L +R
Sbjct:  473 VVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 3 Score: 50.447 bits (119), Expect = 3.189e-8
Identity = 29/106 (27.36%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IV LL++   N    T+    P+H A  +   E+  +LL+ N D      + + P+H A    N E+V LLL+R   ++        P+H A    N ++  +L +
Sbjct:  506 IVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 4 Score: 48.9062 bits (115), Expect = 9.736e-8
Identity = 26/113 (23.01%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +V+LL+E    ++ + K ++ P+H A    N ++  LLLE     +      + P+H A  K   E+ + LL    +       G+ P+H +    + EI  +L +   ++
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDV 682          

HSP 5 Score: 48.1358 bits (113), Expect = 1.760e-7
Identity = 25/90 (27.78%), Postives = 42/90 (46.67%), Query Frame = -2
Query:   58 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 327
            QLL+E   N+  + +  + P+H A     + + NLLL R   I+        P+H A    + ++V+LL+ +   I      G  P+H A
Sbjct:  244 QLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMA 333          

HSP 6 Score: 46.9802 bits (110), Expect = 5.692e-7
Identity = 25/111 (22.52%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +IV  L++   N + +T +   P+H A     +++  +L+     ++ +  +   P+H A    N+++V LLL    N        + P+H A      E+  +L D N +
Sbjct:  439 NIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD 549          

HSP 7 Score: 45.4394 bits (106), Expect = 1.501e-6
Identity = 25/101 (24.75%), Postives = 48/101 (47.52%), Query Frame = -2
Query:   22 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 324
            LL+++  N +  +K    P+H A    +  +  LLLE+  ++  +      P+H A     + M NLLL R   I+        P+H A  + + +++++L
Sbjct:  212 LLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLL 312          

HSP 8 Score: 44.669 bits (104), Expect = 2.875e-6
Identity = 23/111 (20.72%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL+E+  + +   K    P+H A  K   EI + LL+   D   +    + P+H +  + + E+  LL++   ++    N G   +H     ++  + ++L++    I
Sbjct:  605 VAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEI 715          
BLAST of SMED30005565 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 57.7658 bits (138), Expect = 8.534e-11
Identity = 30/105 (28.57%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  +L++HN +    TK+   P+H A    N E+  LLLER   ++ E   +  P+H A +  N ++  LLL+   + +     G+ P+H A      EI   L 
Sbjct:  539 VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 2 Score: 55.0694 bits (131), Expect = 7.590e-10
Identity = 27/107 (25.23%), Postives = 54/107 (50.47%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V++LI +   ++ + ++   P+H A    N++I  LLL+   +        + P+H A  +   E+  +LLD N +      +G+ P+H A    N E++ +L +R
Sbjct:  473 VVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 3 Score: 50.447 bits (119), Expect = 3.189e-8
Identity = 29/106 (27.36%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IV LL++   N    T+    P+H A  +   E+  +LL+ N D      + + P+H A    N E+V LLL+R   ++        P+H A    N ++  +L +
Sbjct:  506 IVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 4 Score: 48.9062 bits (115), Expect = 9.736e-8
Identity = 26/113 (23.01%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +V+LL+E    ++ + K ++ P+H A    N ++  LLLE     +      + P+H A  K   E+ + LL    +       G+ P+H +    + EI  +L +   ++
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDV 682          

HSP 5 Score: 48.1358 bits (113), Expect = 1.760e-7
Identity = 25/90 (27.78%), Postives = 42/90 (46.67%), Query Frame = -2
Query:   58 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 327
            QLL+E   N+  + +  + P+H A     + + NLLL R   I+        P+H A    + ++V+LL+ +   I      G  P+H A
Sbjct:  244 QLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMA 333          

HSP 6 Score: 46.9802 bits (110), Expect = 5.692e-7
Identity = 25/111 (22.52%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +IV  L++   N + +T +   P+H A     +++  +L+     ++ +  +   P+H A    N+++V LLL    N        + P+H A      E+  +L D N +
Sbjct:  439 NIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD 549          

HSP 7 Score: 45.4394 bits (106), Expect = 1.501e-6
Identity = 25/101 (24.75%), Postives = 48/101 (47.52%), Query Frame = -2
Query:   22 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 324
            LL+++  N +  +K    P+H A    +  +  LLLE+  ++  +      P+H A     + M NLLL R   I+        P+H A  + + +++++L
Sbjct:  212 LLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLL 312          

HSP 8 Score: 44.669 bits (104), Expect = 2.875e-6
Identity = 23/111 (20.72%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL+E+  + +   K    P+H A  K   EI + LL+   D   +    + P+H +  + + E+  LL++   ++    N G   +H     ++  + ++L++    I
Sbjct:  605 VAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEI 715          
BLAST of SMED30005565 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 57.7658 bits (138), Expect = 8.538e-11
Identity = 30/105 (28.57%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  +L++HN +    TK+   P+H A    N E+  LLLER   ++ E   +  P+H A +  N ++  LLL+   + +     G+ P+H A      EI   L 
Sbjct:  539 VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 2 Score: 55.0694 bits (131), Expect = 7.593e-10
Identity = 27/107 (25.23%), Postives = 54/107 (50.47%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V++LI +   ++ + ++   P+H A    N++I  LLL+   +        + P+H A  +   E+  +LLD N +      +G+ P+H A    N E++ +L +R
Sbjct:  473 VVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 3 Score: 50.447 bits (119), Expect = 3.190e-8
Identity = 29/106 (27.36%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IV LL++   N    T+    P+H A  +   E+  +LL+ N D      + + P+H A    N E+V LLL+R   ++        P+H A    N ++  +L +
Sbjct:  506 IVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 4 Score: 48.9062 bits (115), Expect = 9.740e-8
Identity = 26/113 (23.01%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +V+LL+E    ++ + K ++ P+H A    N ++  LLLE     +      + P+H A  K   E+ + LL    +       G+ P+H +    + EI  +L +   ++
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDV 682          

HSP 5 Score: 48.1358 bits (113), Expect = 1.760e-7
Identity = 25/90 (27.78%), Postives = 42/90 (46.67%), Query Frame = -2
Query:   58 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 327
            QLL+E   N+  + +  + P+H A     + + NLLL R   I+        P+H A    + ++V+LL+ +   I      G  P+H A
Sbjct:  244 QLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMA 333          

HSP 6 Score: 46.9802 bits (110), Expect = 5.695e-7
Identity = 25/111 (22.52%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +IV  L++   N + +T +   P+H A     +++  +L+     ++ +  +   P+H A    N+++V LLL    N        + P+H A      E+  +L D N +
Sbjct:  439 NIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD 549          

HSP 7 Score: 45.4394 bits (106), Expect = 1.502e-6
Identity = 25/101 (24.75%), Postives = 48/101 (47.52%), Query Frame = -2
Query:   22 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 324
            LL+++  N +  +K    P+H A    +  +  LLLE+  ++  +      P+H A     + M NLLL R   I+        P+H A  + + +++++L
Sbjct:  212 LLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLL 312          

HSP 8 Score: 44.669 bits (104), Expect = 2.876e-6
Identity = 23/111 (20.72%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL+E+  + +   K    P+H A  K   EI + LL+   D   +    + P+H +  + + E+  LL++   ++    N G   +H     ++  + ++L++    I
Sbjct:  605 VAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEI 715          
BLAST of SMED30005565 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 57.7658 bits (138), Expect = 8.538e-11
Identity = 30/105 (28.57%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  +L++HN +    TK+   P+H A    N E+  LLLER   ++ E   +  P+H A +  N ++  LLL+   + +     G+ P+H A      EI   L 
Sbjct:  539 VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 2 Score: 55.0694 bits (131), Expect = 7.593e-10
Identity = 27/107 (25.23%), Postives = 54/107 (50.47%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V++LI +   ++ + ++   P+H A    N++I  LLL+   +        + P+H A  +   E+  +LLD N +      +G+ P+H A    N E++ +L +R
Sbjct:  473 VVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 3 Score: 50.447 bits (119), Expect = 3.190e-8
Identity = 29/106 (27.36%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IV LL++   N    T+    P+H A  +   E+  +LL+ N D      + + P+H A    N E+V LLL+R   ++        P+H A    N ++  +L +
Sbjct:  506 IVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 4 Score: 48.9062 bits (115), Expect = 9.740e-8
Identity = 26/113 (23.01%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +V+LL+E    ++ + K ++ P+H A    N ++  LLLE     +      + P+H A  K   E+ + LL    +       G+ P+H +    + EI  +L +   ++
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDV 682          

HSP 5 Score: 48.1358 bits (113), Expect = 1.760e-7
Identity = 25/90 (27.78%), Postives = 42/90 (46.67%), Query Frame = -2
Query:   58 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 327
            QLL+E   N+  + +  + P+H A     + + NLLL R   I+        P+H A    + ++V+LL+ +   I      G  P+H A
Sbjct:  244 QLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMA 333          

HSP 6 Score: 46.9802 bits (110), Expect = 5.695e-7
Identity = 25/111 (22.52%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +IV  L++   N + +T +   P+H A     +++  +L+     ++ +  +   P+H A    N+++V LLL    N        + P+H A      E+  +L D N +
Sbjct:  439 NIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD 549          

HSP 7 Score: 45.4394 bits (106), Expect = 1.502e-6
Identity = 25/101 (24.75%), Postives = 48/101 (47.52%), Query Frame = -2
Query:   22 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 324
            LL+++  N +  +K    P+H A    +  +  LLLE+  ++  +      P+H A     + M NLLL R   I+        P+H A  + + +++++L
Sbjct:  212 LLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLL 312          

HSP 8 Score: 44.669 bits (104), Expect = 2.876e-6
Identity = 23/111 (20.72%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL+E+  + +   K    P+H A  K   EI + LL+   D   +    + P+H +  + + E+  LL++   ++    N G   +H     ++  + ++L++    I
Sbjct:  605 VAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEI 715          
BLAST of SMED30005565 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 57.7658 bits (138), Expect = 8.542e-11
Identity = 30/105 (28.57%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  +L++HN +    TK+   P+H A    N E+  LLLER   ++ E   +  P+H A +  N ++  LLL+   + +     G+ P+H A      EI   L 
Sbjct:  539 VAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 2 Score: 55.0694 bits (131), Expect = 7.596e-10
Identity = 27/107 (25.23%), Postives = 54/107 (50.47%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V++LI +   ++ + ++   P+H A    N++I  LLL+   +        + P+H A  +   E+  +LLD N +      +G+ P+H A    N E++ +L +R
Sbjct:  473 VVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 3 Score: 50.447 bits (119), Expect = 3.192e-8
Identity = 29/106 (27.36%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IV LL++   N    T+    P+H A  +   E+  +LL+ N D      + + P+H A    N E+V LLL+R   ++        P+H A    N ++  +L +
Sbjct:  506 IVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 4 Score: 48.9062 bits (115), Expect = 9.744e-8
Identity = 26/113 (23.01%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +V+LL+E    ++ + K ++ P+H A    N ++  LLLE     +      + P+H A  K   E+ + LL    +       G+ P+H +    + EI  +L +   ++
Sbjct:  570 LEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDV 682          

HSP 5 Score: 48.1358 bits (113), Expect = 1.761e-7
Identity = 25/90 (27.78%), Postives = 42/90 (46.67%), Query Frame = -2
Query:   58 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 327
            QLL+E   N+  + +  + P+H A     + + NLLL R   I+        P+H A    + ++V+LL+ +   I      G  P+H A
Sbjct:  244 QLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMA 333          

HSP 6 Score: 46.9802 bits (110), Expect = 5.697e-7
Identity = 25/111 (22.52%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +IV  L++   N + +T +   P+H A     +++  +L+     ++ +  +   P+H A    N+++V LLL    N        + P+H A      E+  +L D N +
Sbjct:  439 NIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNAD 549          

HSP 7 Score: 45.4394 bits (106), Expect = 1.503e-6
Identity = 25/101 (24.75%), Postives = 48/101 (47.52%), Query Frame = -2
Query:   22 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 324
            LL+++  N +  +K    P+H A    +  +  LLLE+  ++  +      P+H A     + M NLLL R   I+        P+H A  + + +++++L
Sbjct:  212 LLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLL 312          

HSP 8 Score: 44.669 bits (104), Expect = 2.878e-6
Identity = 23/111 (20.72%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL+E+  + +   K    P+H A  K   EI + LL+   D   +    + P+H +  + + E+  LL++   ++    N G   +H     ++  + ++L++    I
Sbjct:  605 VAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEI 715          
BLAST of SMED30005565 vs. Ensembl Fly
Match: Atac3 (gene:FBgn0052343 transcript:FBtr0299838)

HSP 1 Score: 63.929 bits (154), Expect = 7.453e-13
Identity = 33/106 (31.13%), Postives = 53/106 (50.00%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
              I ++L++   N++ KTK    P+H A    +  I +LLL   C +   D  R  P+HWA+ KK+  +V LLL    ++      G  PI  A+  E +++L  L
Sbjct:   99 LEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLLKCQADVTLVSKFGKTPIGLAVYTEQADVLAEL 204          

HSP 2 Score: 45.4394 bits (106), Expect = 1.791e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = -2
Query:   19 LRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 276
            +  +HFA M    EI  +LL+   +++ +      P+H A    +  +V+LLL   C++   D     P+HWA+  ++  I+ +L 
Sbjct:   87 MSALHFAAMNNQLEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLL 172          
BLAST of SMED30005565 vs. Ensembl Fly
Match: Atac3 (gene:FBgn0052343 transcript:FBtr0299831)

HSP 1 Score: 63.929 bits (154), Expect = 7.453e-13
Identity = 33/106 (31.13%), Postives = 53/106 (50.00%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
              I ++L++   N++ KTK    P+H A    +  I +LLL   C +   D  R  P+HWA+ KK+  +V LLL    ++      G  PI  A+  E +++L  L
Sbjct:   99 LEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLLKCQADVTLVSKFGKTPIGLAVYTEQADVLAEL 204          

HSP 2 Score: 45.4394 bits (106), Expect = 1.791e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = -2
Query:   19 LRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 276
            +  +HFA M    EI  +LL+   +++ +      P+H A    +  +V+LLL   C++   D     P+HWA+  ++  I+ +L 
Sbjct:   87 MSALHFAAMNNQLEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLL 172          
BLAST of SMED30005565 vs. Ensembl Fly
Match: Atac3 (gene:FBgn0052343 transcript:FBtr0309236)

HSP 1 Score: 63.929 bits (154), Expect = 7.536e-13
Identity = 33/106 (31.13%), Postives = 53/106 (50.00%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
              I ++L++   N++ KTK    P+H A    +  I +LLL   C +   D  R  P+HWA+ KK+  +V LLL    ++      G  PI  A+  E +++L  L
Sbjct:   99 LEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLLKCQADVTLVSKFGKTPIGLAVYTEQADVLAEL 204          

HSP 2 Score: 45.4394 bits (106), Expect = 1.793e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = -2
Query:   19 LRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 276
            +  +HFA M    EI  +LL+   +++ +      P+H A    +  +V+LLL   C++   D     P+HWA+  ++  I+ +L 
Sbjct:   87 MSALHFAAMNNQLEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLL 172          
BLAST of SMED30005565 vs. Ensembl Fly
Match: Atac3 (gene:FBgn0052343 transcript:FBtr0332840)

HSP 1 Score: 63.929 bits (154), Expect = 7.536e-13
Identity = 33/106 (31.13%), Postives = 53/106 (50.00%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
              I ++L++   N++ KTK    P+H A    +  I +LLL   C +   D  R  P+HWA+ KK+  +V LLL    ++      G  PI  A+  E +++L  L
Sbjct:   99 LEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLLKCQADVTLVSKFGKTPIGLAVYTEQADVLAEL 204          

HSP 2 Score: 45.4394 bits (106), Expect = 1.793e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = -2
Query:   19 LRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 276
            +  +HFA M    EI  +LL+   +++ +      P+H A    +  +V+LLL   C++   D     P+HWA+  ++  I+ +L 
Sbjct:   87 MSALHFAAMNNQLEICEILLQGGINMDGKTKVDRTPLHLACYYGHERIVSLLLALKCSVNSRDMLRMTPLHWAVEKKHKGIVRLLL 172          
BLAST of SMED30005565 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334062)

HSP 1 Score: 62.3882 bits (150), Expect = 2.200e-12
Identity = 32/108 (29.63%), Postives = 56/108 (51.85%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N +  +K    P+H A    N  I NLL+++  D+         P+H A     + MV+LLL++  NIE     G  P+H A  + + ++++ML +R   I
Sbjct:  190 LLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPI 297          

HSP 2 Score: 53.9138 bits (128), Expect = 2.374e-9
Identity = 28/106 (26.42%), Postives = 51/106 (48.11%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            +I  LLI+   ++    K  + P+H A     + + +LLLE+  +IE +      P+H A    + ++V++LL+R   I      G  P+H A   E+ +   +L 
Sbjct:  219 NIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324          

HSP 3 Score: 48.9062 bits (115), Expect = 1.223e-7
Identity = 34/145 (23.45%), Postives = 58/145 (40.00%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSE---------------------------------MVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++V LL+E   NIE KT+  L P+H A    + ++ ++LLER   I  +      P+H A   ++ +                                 +  LLLDRN +       G+ P+H A      +++E+L     +I
Sbjct:  252 NMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASI 396          

HSP 4 Score: 43.5134 bits (101), Expect = 8.821e-6
Identity = 23/108 (21.30%), Postives = 49/108 (45.37%), Query Frame = -2
Query:   10 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 333
            +V  L+     ++  TK+    +H A +    E+  LLLE N  +  +    + P++ A  + +  +V LLL    N       G+ P+  A+   + +++ +L + +
Sbjct:   59 VVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESD 166          
BLAST of SMED30005565 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 63.929 bits (154), Expect = 9.467e-13
Identity = 34/108 (31.48%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N  MV LLLDR   IE     G  P+H    + + +++EML DR   I
Sbjct:  221 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 328          

HSP 2 Score: 55.8398 bits (133), Expect = 7.490e-10
Identity = 27/105 (25.71%), Postives = 48/105 (45.71%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+    N+    K  L P+H A   + + +  +LL    +I+ +    + P+H A +  N +M N LL+           G+ P+H A    ++ I+ ML 
Sbjct:  680 LVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLL 784          

HSP 3 Score: 54.299 bits (129), Expect = 2.180e-9
Identity = 29/111 (26.13%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   EI   LLE   +      Q   P+H A  + + ++V+LLL +  N+  G+  G  P+H A  ++ + + E+L +    I
Sbjct:  614 VALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEI 724          

HSP 4 Score: 52.373 bits (124), Expect = 1.061e-8
Identity = 26/112 (23.21%), Postives = 50/112 (44.64%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++  LL+     ++   +  + P+H A  + N  +  LLL+R   IE +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N  +
Sbjct:  250 NVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPV 361          

HSP 5 Score: 48.9062 bits (115), Expect = 1.789e-7
Identity = 30/111 (27.03%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N  ++++L DR   I
Sbjct:  189 VVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKI 295          

HSP 6 Score: 48.521 bits (114), Expect = 2.302e-7
Identity = 31/112 (27.68%), Postives = 48/112 (42.86%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD--RNCN 333
            I  LL+E   ++   TK+   P+H A      E+ NLLL++    +        P+H A +  N  +  LLLD+  +       G+ P+H A      EI   L +    CN
Sbjct:  548 IAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECN 659          

HSP 7 Score: 47.7506 bits (112), Expect = 4.041e-7
Identity = 28/109 (25.69%), Postives = 47/109 (43.12%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              IVQ L++        T     P+H +  + + EI  LLLE+   +     + + P+H A      E+ NLLL +    +     G  P+H A   +N  +  +L D+
Sbjct:  513 LEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQ 621          

HSP 8 Score: 47.7506 bits (112), Expect = 5.101e-7
Identity = 25/111 (22.52%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +++D+  N       G+ P+H A      +++E+L     ++
Sbjct:  317 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASL 427          

HSP 9 Score: 47.3654 bits (111), Expect = 5.900e-7
Identity = 28/111 (25.23%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    IE KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ +++ D+  N
Sbjct:  283 NMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKAN 393          

HSP 10 Score: 44.669 bits (104), Expect = 4.935e-6
Identity = 22/102 (21.57%), Postives = 47/102 (46.08%), Query Frame = -2
Query:   16 LIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 321
            L+E+       T+Q + P+H A  + + ++ +LLL +  ++   +     P+H A     + +  +LL+    I+     G+ P+H A    N ++   L +
Sbjct:  651 LLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLE 752          
BLAST of SMED30005565 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 63.929 bits (154), Expect = 9.467e-13
Identity = 34/108 (31.48%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N  MV LLLDR   IE     G  P+H    + + +++EML DR   I
Sbjct:  221 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 328          

HSP 2 Score: 55.8398 bits (133), Expect = 7.490e-10
Identity = 27/105 (25.71%), Postives = 48/105 (45.71%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+    N+    K  L P+H A   + + +  +LL    +I+ +    + P+H A +  N +M N LL+           G+ P+H A    ++ I+ ML 
Sbjct:  680 LVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLL 784          

HSP 3 Score: 54.299 bits (129), Expect = 2.180e-9
Identity = 29/111 (26.13%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   EI   LLE   +      Q   P+H A  + + ++V+LLL +  N+  G+  G  P+H A  ++ + + E+L +    I
Sbjct:  614 VALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEI 724          

HSP 4 Score: 52.373 bits (124), Expect = 1.061e-8
Identity = 26/112 (23.21%), Postives = 50/112 (44.64%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++  LL+     ++   +  + P+H A  + N  +  LLL+R   IE +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N  +
Sbjct:  250 NVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPV 361          

HSP 5 Score: 48.9062 bits (115), Expect = 1.789e-7
Identity = 30/111 (27.03%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N  ++++L DR   I
Sbjct:  189 VVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKI 295          

HSP 6 Score: 48.521 bits (114), Expect = 2.302e-7
Identity = 31/112 (27.68%), Postives = 48/112 (42.86%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD--RNCN 333
            I  LL+E   ++   TK+   P+H A      E+ NLLL++    +        P+H A +  N  +  LLLD+  +       G+ P+H A      EI   L +    CN
Sbjct:  548 IAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECN 659          

HSP 7 Score: 47.7506 bits (112), Expect = 4.041e-7
Identity = 28/109 (25.69%), Postives = 47/109 (43.12%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              IVQ L++        T     P+H +  + + EI  LLLE+   +     + + P+H A      E+ NLLL +    +     G  P+H A   +N  +  +L D+
Sbjct:  513 LEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQ 621          

HSP 8 Score: 47.7506 bits (112), Expect = 5.101e-7
Identity = 25/111 (22.52%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +++D+  N       G+ P+H A      +++E+L     ++
Sbjct:  317 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASL 427          

HSP 9 Score: 47.3654 bits (111), Expect = 5.900e-7
Identity = 28/111 (25.23%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    IE KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ +++ D+  N
Sbjct:  283 NMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKAN 393          

HSP 10 Score: 44.669 bits (104), Expect = 4.935e-6
Identity = 22/102 (21.57%), Postives = 47/102 (46.08%), Query Frame = -2
Query:   16 LIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 321
            L+E+       T+Q + P+H A  + + ++ +LLL +  ++   +     P+H A     + +  +LL+    I+     G+ P+H A    N ++   L +
Sbjct:  651 LLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLE 752          
BLAST of SMED30005565 vs. Ensembl Zebrafish
Match: BX469921.1 (ankyrin 3a [Source:NCBI gene;Acc:103909035])

HSP 1 Score: 63.929 bits (154), Expect = 9.468e-13
Identity = 34/108 (31.48%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N  MV LLLDR   IE     G  P+H    + + +++EML DR   I
Sbjct:  221 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 328          

HSP 2 Score: 54.299 bits (129), Expect = 2.180e-9
Identity = 29/111 (26.13%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   EI   LLE   +      Q   P+H A  + + ++V+LLL +  N+  G+  G  P+H A  ++ + + E+L +    I
Sbjct:  614 VALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEI 724          

HSP 3 Score: 52.373 bits (124), Expect = 1.061e-8
Identity = 26/112 (23.21%), Postives = 50/112 (44.64%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++  LL+     ++   +  + P+H A  + N  +  LLL+R   IE +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N  +
Sbjct:  250 NVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPV 361          

HSP 4 Score: 48.9062 bits (115), Expect = 1.789e-7
Identity = 30/111 (27.03%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N  ++++L DR   I
Sbjct:  189 VVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKI 295          

HSP 5 Score: 48.521 bits (114), Expect = 2.302e-7
Identity = 31/112 (27.68%), Postives = 48/112 (42.86%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD--RNCN 333
            I  LL+E   ++   TK+   P+H A      E+ NLLL++    +        P+H A +  N  +  LLLD+  +       G+ P+H A      EI   L +    CN
Sbjct:  548 IAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECN 659          

HSP 6 Score: 47.7506 bits (112), Expect = 4.042e-7
Identity = 28/109 (25.69%), Postives = 47/109 (43.12%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              IVQ L++        T     P+H +  + + EI  LLLE+   +     + + P+H A      E+ NLLL +    +     G  P+H A   +N  +  +L D+
Sbjct:  513 LEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQ 621          

HSP 7 Score: 47.7506 bits (112), Expect = 5.102e-7
Identity = 25/111 (22.52%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +++D+  N       G+ P+H A      +++E+L     ++
Sbjct:  317 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASL 427          

HSP 8 Score: 47.3654 bits (111), Expect = 5.901e-7
Identity = 28/111 (25.23%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    IE KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ +++ D+  N
Sbjct:  283 NMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKAN 393          

HSP 9 Score: 44.669 bits (104), Expect = 4.936e-6
Identity = 22/102 (21.57%), Postives = 47/102 (46.08%), Query Frame = -2
Query:   16 LIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 321
            L+E+       T+Q + P+H A  + + ++ +LLL +  ++   +     P+H A     + +  +LL+    I+     G+ P+H A    N ++   L +
Sbjct:  651 LLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLE 752          

HSP 10 Score: 43.8986 bits (102), Expect = 9.450e-6
Identity = 20/73 (27.40%), Postives = 36/73 (49.32%), Query Frame = -2
Query:  115 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLD 333
            +V LL+    N+    K  L P+H A   + + +  +LL    +I+ +    + P+H A +  N +M N LL+
Sbjct:  680 LVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLE 752          
BLAST of SMED30005565 vs. Ensembl Zebrafish
Match: BX511162.1 (ankyrin 2a, neuronal [Source:NCBI gene;Acc:568926])

HSP 1 Score: 62.3882 bits (150), Expect = 1.997e-12
Identity = 31/111 (27.93%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+EH    + K K +L  +H A  K++++   LLL+ + + + +    + P+H A +  N  +  LLL+R   ++     G  P+H A    N+ ++ +L DR   I
Sbjct:  165 VVSLLLEH----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMIALLLDRGSQI 271          

HSP 2 Score: 51.9878 bits (123), Expect = 1.036e-8
Identity = 23/77 (29.87%), Postives = 39/77 (50.65%), Query Frame = -2
Query:   94 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIEC 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ M+ LLLDR   I+ 
Sbjct:  197 LLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMIALLLDRGSQIDA 273          
BLAST of SMED30005565 vs. Ensembl Zebrafish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 62.003 bits (149), Expect = 4.296e-12
Identity = 32/108 (29.63%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++ +      P+H A  + N+ MV LLL+R   I+     G  P+H    + + +++EML DR   I
Sbjct:  191 LLLQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 298          

HSP 2 Score: 52.373 bits (124), Expect = 9.511e-9
Identity = 26/105 (24.76%), Postives = 49/105 (46.67%), Query Frame = -2
Query:   22 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 336
            ++  LL+     ++ K +  + P+H A  + N+ +  LLLER   I+        P+H      + ++V +LLDR   I      G  P+H A   ++   +++L
Sbjct:  220 NVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLL 324          

HSP 3 Score: 48.1358 bits (113), Expect = 3.014e-7
Identity = 29/111 (26.13%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++ +L +R   I
Sbjct:  159 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARI 265          

HSP 4 Score: 44.669 bits (104), Expect = 4.649e-6
Identity = 25/111 (22.52%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LL+     ++   N     +H A +  + ++  +L+D+  N       G+ P+H A      +++E+L     +I
Sbjct:  287 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 397          
BLAST of SMED30005565 vs. Ensembl Xenopus
Match: gorasp1 (golgi reassembly stacking protein 1 [Source:Xenbase;Acc:XB-GENE-976361])

HSP 1 Score: 68.5514 bits (166), Expect = 3.062e-14
Identity = 31/93 (33.33%), Postives = 51/93 (54.84%), Query Frame = -2
Query:   55 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAI 333
            I +LLI+H  N + K+ Q   P+H A  K ++EI NLL +   +I+ E + +W P+H A+      +V+ LL    + E  +  GW  +H A+
Sbjct:  572 ICKLLIKHGANADAKSFQDWTPLHLATYKGHTEIINLLKDGGSNIDSEGDLKWTPLHLAVRYSEELVVSHLLTLGADPEIAEMSGWTALHLAV 664          

HSP 2 Score: 66.2402 bits (160), Expect = 1.958e-13
Identity = 30/95 (31.58%), Postives = 50/95 (52.63%), Query Frame = -2
Query:   55 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAI 339
            F + Q LI+   N+    +     +H A +K NS I  LL++   + + +  Q W P+H A  K ++E++NLL D   NI+   +  W P+H A+
Sbjct:  537 FRVAQHLIQKGANLNFPDQSNYTALHMAAVKGNSMICKLLIKHGANADAKSFQDWTPLHLATYKGHTEIINLLKDGGSNIDSEGDLKWTPLHLAV 631          

HSP 3 Score: 62.7734 bits (151), Expect = 3.246e-12
Identity = 30/102 (29.41%), Postives = 50/102 (49.02%), Query Frame = -2
Query:    4 NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 309
            N NI  K+     P+  A+ K+  +I   L+E   D+   D   W P+H+A    +  +V LLLD + N++  +   W  +H A  N    ++ +LF R+ N
Sbjct:  382 NLNINSKSISGFTPLILAVQKKLPDICMCLIENGADVNITDEDMWSPLHFAAQGGDDRIVRLLLDHSANVDSKERDDWTALHLAAQNGYENVVRVLFTRHTN 483          

HSP 4 Score: 56.225 bits (134), Expect = 6.920e-10
Identity = 28/113 (24.78%), Postives = 51/113 (45.13%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            + +V+LLI    N+      +   +H A  K    +   L+++  ++   D   +  +H A  K NS +  LL+    N +    + W P+H A    ++EI+ +L D   NI
Sbjct:  504 YKLVKLLISQGANVNSIQNDQRTALHIAADKGYFRVAQHLIQKGANLNFPDQSNYTALHMAAVKGNSMICKLLIKHGANADAKSFQDWTPLHLATYKGHTEIINLLKDGGSNI 616          

HSP 5 Score: 49.2914 bits (116), Expect = 2.047e-7
Identity = 23/107 (21.50%), Postives = 50/107 (46.73%), Query Frame = -2
Query:    1 LIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 321
            LIE+  ++    +    P+HFA    +  I  LLL+ + +++ ++   W  +H A       +V +L  R+ N    +  G   +H A    + +++++L  +  N+
Sbjct:  411 LIENGADVNITDEDMWSPLHFAAQGGDDRIVRLLLDHSANVDSKERDDWTALHLAAQNGYENVVRVLFTRHTNPNSQEVNGKTALHLATYFGHYKLVKLLISQGANV 517          

HSP 6 Score: 44.669 bits (104), Expect = 6.601e-6
Identity = 22/89 (24.72%), Postives = 43/89 (48.31%), Query Frame = -2
Query:    1 IHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 267
            +H A+ + N E  N  L  N +I  +    + P+  A+ KK  ++   L++   ++   D   W P+H+A    +  I+ +L D + N+
Sbjct:  363 LHVAVAQGNLEKVNHFLSLNLNINSKSISGFTPLILAVQKKLPDICMCLIENGADVNITDEDMWSPLHFAAQGGDDRIVRLLLDHSANV 451          
BLAST of SMED30005565 vs. Ensembl Xenopus
Match: cpsf1 (cleavage and polyadenylation specific factor 1 [Source:Xenbase;Acc:XB-GENE-5881751])

HSP 1 Score: 65.4698 bits (158), Expect = 2.658e-13
Identity = 30/106 (28.30%), Postives = 54/106 (50.94%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
             S V+LLI+    + C+      P+H A    ++ +  LLL+R  +          P+H A +  ++ +  LLL +N +   GDN  W P+HWA ++ +  I+E++
Sbjct:   81 LSTVKLLIQRGALLSCQDGSGCTPLHHAAHNGHTHLCELLLQRGANPGATTKGGLTPLHLAASNGHTLITQLLLKQNLDPCVGDNNQWSPLHWATVSNHFHIIELM 186          

HSP 2 Score: 58.9214 bits (141), Expect = 6.556e-11
Identity = 30/109 (27.52%), Postives = 51/109 (46.79%), Query Frame = -2
Query:    1 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 327
            +LL++   N    TK  L P+H A    ++ I  LLL++N D    DN +W P+HWA    +  ++ L+ +    ++   +    P+H A      E L  L D   ++
Sbjct:  118 ELLLQRGANPGATTKGGLTPLHLAASNGHTLITQLLLKQNLDPCVGDNNQWSPLHWATVSNHFHIIELMKEYGVPLDLEASGNLAPLHIAAETGRLEALGYLLDNRADV 226          

HSP 3 Score: 52.373 bits (124), Expect = 1.249e-8
Identity = 25/98 (25.51%), Postives = 44/98 (44.90%), Query Frame = -2
Query:    4 ECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 297
             C   ++ + +H A    N+ +   LL++   ++  D   W  +H A    +   V LL+ R   + C D  G  P+H A  N ++ + E+L  R  N
Sbjct:   29 SCPGVKEWKTLHLAAWNGNTRLVKQLLQQGEAVDSRDGLGWTSLHCAAANGHLSTVKLLIQRGALLSCQDGSGCTPLHHAAHNGHTHLCELLLQRGAN 126          

HSP 4 Score: 51.9878 bits (123), Expect = 1.937e-8
Identity = 29/111 (26.13%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I QLL++ N +       +  P+H+A +  +  I  L+ E    ++ E +    P+H A      E +  LLD   ++   D  G  P+  A  N N E++ +L ++N  I
Sbjct:  149 ITQLLLKQNLDPCVGDNNQWSPLHWATVSNHFHIIELMKEYGVPLDLEASGNLAPLHIAAETGRLEALGYLLDNRADVNVKDQLGRTPLAIAAGNGNLEVVRLLLEKNAEI 259          

HSP 5 Score: 51.2174 bits (121), Expect = 3.247e-8
Identity = 25/108 (23.15%), Postives = 49/108 (45.37%), Query Frame = -2
Query:   10 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 333
            +V+ L++    ++ +       +H A    +     LL++R   + C+D     P+H A +  ++ +  LLL R  N       G  P+H A  N ++ I ++L  +N
Sbjct:   50 LVKQLLQQGEAVDSRDGLGWTSLHCAAANGHLSTVKLLIQRGALLSCQDGSGCTPLHHAAHNGHTHLCELLLQRGANPGATTKGGLTPLHLAASNGHTLITQLLLKQN 157          
BLAST of SMED30005565 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182])

HSP 1 Score: 62.003 bits (149), Expect = 6.939e-12
Identity = 31/111 (27.93%), Postives = 60/111 (54.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++H+ ++E + K   R +H A   +   +  +L     D+   + +R  P+H A+NK + ++V  LLD  C+    D+ G  P+H AI  +  +IL +L +   ++
Sbjct:  491 ILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADV 601          

HSP 2 Score: 53.9138 bits (128), Expect = 4.743e-9
Identity = 25/108 (23.15%), Postives = 59/108 (54.63%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            ++V++L     ++  + K++  P+H A+ K + ++   LL+  C    +D++   P+H AI+KK  +++ +LL+   ++   +N G+  +H A +  N   + +L  +
Sbjct:  523 TVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSK 630          
BLAST of SMED30005565 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182])

HSP 1 Score: 62.003 bits (149), Expect = 7.269e-12
Identity = 31/111 (27.93%), Postives = 60/111 (54.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++H+ ++E + K   R +H A   +   +  +L     D+   + +R  P+H A+NK + ++V  LLD  C+    D+ G  P+H AI  +  +IL +L +   ++
Sbjct:  479 ILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADV 589          

HSP 2 Score: 53.9138 bits (128), Expect = 4.873e-9
Identity = 25/108 (23.15%), Postives = 59/108 (54.63%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            ++V++L     ++  + K++  P+H A+ K + ++   LL+  C    +D++   P+H AI+KK  +++ +LL+   ++   +N G+  +H A +  N   + +L  +
Sbjct:  511 TVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSK 618          
BLAST of SMED30005565 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097])

HSP 1 Score: 60.4622 bits (145), Expect = 2.113e-11
Identity = 32/108 (29.63%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++ ML DR   I
Sbjct:  203 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPI 310          

HSP 2 Score: 52.373 bits (124), Expect = 1.457e-8
Identity = 30/111 (27.03%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q   P+H A  + + +MV+LLL R+ N+   +  G  P+H A   +   + E+L ++   I
Sbjct:  596 VALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVI 706          

HSP 3 Score: 51.6026 bits (122), Expect = 2.848e-8
Identity = 25/109 (22.94%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 4 Score: 50.447 bits (119), Expect = 7.739e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKI 277          

HSP 5 Score: 50.0618 bits (118), Expect = 1.066e-7
Identity = 26/111 (23.42%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V+LL++ N + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G  P+H A    + +++ +L  R+ N+
Sbjct:  563 VVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANV 673          

HSP 6 Score: 49.6766 bits (117), Expect = 1.512e-7
Identity = 27/106 (25.47%), Postives = 47/106 (44.34%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +  +L+EH  +    TK+   P+H A      E+  LLL++N   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  530 VASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLE 635          

HSP 7 Score: 48.9062 bits (115), Expect = 2.629e-7
Identity = 28/111 (25.23%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V +L++    I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  N       G+ P+H A      +++E+L     +I
Sbjct:  299 VVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASI 409          

HSP 8 Score: 48.521 bits (114), Expect = 3.586e-7
Identity = 25/105 (23.81%), Postives = 47/105 (44.76%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+  + N+    K  L P+H A  ++   +  +L  +   I+      + P+H A +  N +MV  LL     +      G+ P+H A    ++ I+ +L 
Sbjct:  662 MVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 766          

HSP 9 Score: 46.595 bits (109), Expect = 1.493e-6
Identity = 26/107 (24.30%), Postives = 50/107 (46.73%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++     +  T     P+H +  + + ++ ++LLE          + + P+H A      E+V LLL +N + +     G  P+H A   +N ++  +L D+
Sbjct:  497 IVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDK 603          

HSP 10 Score: 46.2098 bits (108), Expect = 2.137e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  N
Sbjct:  265 NMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAN 375          
BLAST of SMED30005565 vs. Ensembl Mouse
Match: Ankk1 (ankyrin repeat and kinase domain containing 1 [Source:MGI Symbol;Acc:MGI:3045301])

HSP 1 Score: 66.2402 bits (160), Expect = 1.509e-13
Identity = 29/110 (26.36%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL+ H+ +++C+T     P+  A   +  ++  LLL    D    D   W P+H+A    +     LLLD    +   ++ GW P+H A  N    +  +L  R  ++
Sbjct:  386 VRLLLSHDVDVDCQTASGYTPLLIATQDQQPDLCALLLAHGADTNLADEDGWAPLHFAAQNGDDHTARLLLDHGALVNAREHEGWTPLHLAAQNNFENVARLLVSRQADL 495          

HSP 2 Score: 55.4546 bits (132), Expect = 7.939e-10
Identity = 25/93 (26.88%), Postives = 49/93 (52.69%), Query Frame = -2
Query:   55 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAI 333
            I ++L+ +  ++E +T+Q   P+H A  K + EI + L + + D++   + +W P+H A  +    ++  LL    N    +  GW P+H A+
Sbjct:  583 IFKMLLRYGASLELRTQQGWTPLHLATYKGHLEIIHQLAKSHVDLDALGSMQWTPLHLAAFQGEEGVMLALLQCGANPNAAEQSGWTPLHLAV 675          

HSP 3 Score: 52.373 bits (124), Expect = 1.041e-8
Identity = 23/90 (25.56%), Postives = 43/90 (47.78%), Query Frame = -2
Query:   16 KQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 285
            + ++ P+HF +   + E   LLL  + D++C+    + P+  A   +  ++  LLL    +    D  GW P+H+A  N +     +L D
Sbjct:  368 ENRVTPLHFLVAGGSLEQVRLLLSHDVDVDCQTASGYTPLLIATQDQQPDLCALLLAHGADTNLADEDGWAPLHFAAQNGDDHTARLLLD 457          

HSP 4 Score: 45.4394 bits (106), Expect = 3.417e-6
Identity = 20/71 (28.17%), Postives = 35/71 (49.30%), Query Frame = -2
Query:   58 PIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 270
            P+H A  +    IF +LL     +E    Q W P+H A  K + E+++ L   + +++   +  W P+H A
Sbjct:  571 PLHIAAARGKDLIFKMLLRYGASLELRTQQGWTPLHLATYKGHLEIIHQLAKSHVDLDALGSMQWTPLHLA 641          
BLAST of SMED30005565 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 64.6994 bits (156), Expect = 5.951e-13
Identity = 34/113 (30.09%), Postives = 61/113 (53.98%), Query Frame = -2
Query:    7 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLL-----DRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNC 330
            V LL+E   N++         +H  IM  + E   +LLE+   I C+D++   P+H+A  + ++  +N LL     + +C ++  DN+G+ P+HWA  N N   +E+L ++ C
Sbjct:  704 VSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGHATWLNELLQIALSEEDCCLK--DNQGYTPLHWACYNGNENCIEVLLEQKC 814          

HSP 2 Score: 51.9878 bits (123), Expect = 1.538e-8
Identity = 25/89 (28.09%), Postives = 46/89 (51.69%), Query Frame = -2
Query:    1 IHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 267
            +H A +  + E+ NLLL +  +I   D +  R +HWA    + ++V LL++    + C D +G+ P+H A  N    +++ L +    I
Sbjct:  144 LHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEI 232          

HSP 3 Score: 49.2914 bits (116), Expect = 1.366e-7
Identity = 27/110 (24.55%), Postives = 52/110 (47.27%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            VQ+LI+H+ ++  + K    P+H A   +  +   +++     +   D      +H A    + EMVNLLL +  NI   D +  R +HWA    + +++ +L +    +
Sbjct:   90 VQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 4 Score: 48.521 bits (114), Expect = 2.399e-7
Identity = 26/111 (23.42%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LLI     +  K    L P+H A+   + E   +L++ + D+   D     P+H A   K  +   +++    ++   D  G   +H A +N + E++ +L  +  NI
Sbjct:   56 IIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANI 166          

HSP 5 Score: 47.3654 bits (111), Expect = 5.746e-7
Identity = 24/103 (23.30%), Postives = 50/103 (48.54%), Query Frame = -2
Query:   22 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 330
            +++LI    ++     +K  P+H A    ++EI  LL+     +  +DN    P+H A+  ++ E V +L+  + ++   D     P+H A  N+  +  E++
Sbjct:   24 IRMLIHKTEDVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVI 126          

HSP 6 Score: 46.2098 bits (108), Expect = 1.591e-6
Identity = 29/114 (25.44%), Postives = 52/114 (45.61%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEI-LEMLFDRNCNI 339
              +V LLI H   + CK K+   P+H A       +   LL    +I+  +      +H A       +VN L+D   N+   +N G+ P+H+A  + +  + LE+L +   ++
Sbjct:  186 LDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANVNQPNNSGFTPLHFAAASTHGALCLELLVNNGADV 299          

HSP 7 Score: 45.0542 bits (105), Expect = 4.197e-6
Identity = 27/111 (24.32%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+    NI    K+  R +H+A    + ++  LL+    ++ C+D + + P+H A +     +V  LL+    I+  +  G   +H A  N    ++  L D   N+
Sbjct:  155 MVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          
BLAST of SMED30005565 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 64.6994 bits (156), Expect = 6.220e-13
Identity = 33/108 (30.56%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR+  I
Sbjct:  203 LLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPI 310          

HSP 2 Score: 53.5286 bits (127), Expect = 4.049e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  596 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 706          

HSP 3 Score: 52.7582 bits (125), Expect = 7.611e-9
Identity = 24/110 (21.82%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+  +H A    ++ I+ +L   N +
Sbjct:  662 MVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 4 Score: 51.9878 bits (123), Expect = 1.754e-8
Identity = 25/109 (22.94%), Postives = 51/109 (46.79%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR+  I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 5 Score: 50.8322 bits (120), Expect = 3.495e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 277          

HSP 6 Score: 48.521 bits (114), Expect = 2.956e-7
Identity = 27/111 (24.32%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++ +  I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  +       G+ P+H A       ++E+L     +I
Sbjct:  299 VVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASI 409          

HSP 7 Score: 47.3654 bits (111), Expect = 5.774e-7
Identity = 25/106 (23.58%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ +LLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  530 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLE 635          

HSP 8 Score: 46.595 bits (109), Expect = 1.242e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  629 IATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 739          

HSP 9 Score: 45.4394 bits (106), Expect = 3.152e-6
Identity = 22/110 (20.00%), Postives = 49/110 (44.55%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V+ L++    +E K K    P+H +     ++I   LL++           + P+H A  + + ++   LLD   ++     +G+ P+H A      E+  +L  ++ +
Sbjct:  464 VVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS 573          

HSP 10 Score: 45.0542 bits (105), Expect = 4.646e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H A  + + ++   LL+    +     + + P+H A      E+ +LLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  497 IVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 603          

HSP 11 Score: 44.669 bits (104), Expect = 5.021e-6
Identity = 26/111 (23.42%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R+  I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  +
Sbjct:  265 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAS 375          

HSP 12 Score: 44.669 bits (104), Expect = 5.426e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  561 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANV 673          
BLAST of SMED30005565 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 64.6994 bits (156), Expect = 6.224e-13
Identity = 33/108 (30.56%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR+  I
Sbjct:  214 LLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPI 321          

HSP 2 Score: 53.5286 bits (127), Expect = 4.051e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  607 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 717          

HSP 3 Score: 52.7582 bits (125), Expect = 7.616e-9
Identity = 24/110 (21.82%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+  +H A    ++ I+ +L   N +
Sbjct:  673 MVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 782          

HSP 4 Score: 51.9878 bits (123), Expect = 1.755e-8
Identity = 25/109 (22.94%), Postives = 51/109 (46.79%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR+  I      G  P+H A   ++   +++L   N
Sbjct:  243 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 351          

HSP 5 Score: 50.8322 bits (120), Expect = 3.497e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  182 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 288          

HSP 6 Score: 48.521 bits (114), Expect = 2.958e-7
Identity = 27/111 (24.32%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++ +  I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  +       G+ P+H A       ++E+L     +I
Sbjct:  310 VVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASI 420          

HSP 7 Score: 47.3654 bits (111), Expect = 5.777e-7
Identity = 25/106 (23.58%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ +LLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  541 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLE 646          

HSP 8 Score: 46.595 bits (109), Expect = 1.243e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  640 IATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 750          

HSP 9 Score: 45.4394 bits (106), Expect = 3.154e-6
Identity = 22/110 (20.00%), Postives = 49/110 (44.55%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V+ L++    +E K K    P+H +     ++I   LL++           + P+H A  + + ++   LLD   ++     +G+ P+H A      E+  +L  ++ +
Sbjct:  475 VVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS 584          

HSP 10 Score: 45.0542 bits (105), Expect = 4.649e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H A  + + ++   LL+    +     + + P+H A      E+ +LLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  508 IVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 614          

HSP 11 Score: 44.669 bits (104), Expect = 5.024e-6
Identity = 26/111 (23.42%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R+  I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  +
Sbjct:  276 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAS 386          

HSP 12 Score: 44.669 bits (104), Expect = 5.429e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  572 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANV 684          
BLAST of SMED30005565 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 64.6994 bits (156), Expect = 6.229e-13
Identity = 33/108 (30.56%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR+  I
Sbjct:  203 LLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPI 310          

HSP 2 Score: 53.5286 bits (127), Expect = 4.055e-9
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  596 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 706          

HSP 3 Score: 52.7582 bits (125), Expect = 7.623e-9
Identity = 24/110 (21.82%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+  +H A    ++ I+ +L   N +
Sbjct:  662 MVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 4 Score: 51.9878 bits (123), Expect = 1.757e-8
Identity = 25/109 (22.94%), Postives = 51/109 (46.79%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR+  I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 5 Score: 50.8322 bits (120), Expect = 3.500e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 277          

HSP 6 Score: 48.521 bits (114), Expect = 2.961e-7
Identity = 27/111 (24.32%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++ +  I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  +       G+ P+H A       ++E+L     +I
Sbjct:  299 VVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASI 409          

HSP 7 Score: 47.3654 bits (111), Expect = 5.783e-7
Identity = 25/106 (23.58%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ +LLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  530 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLE 635          

HSP 8 Score: 46.595 bits (109), Expect = 1.244e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  629 IATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 739          

HSP 9 Score: 45.4394 bits (106), Expect = 3.157e-6
Identity = 22/110 (20.00%), Postives = 49/110 (44.55%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V+ L++    +E K K    P+H +     ++I   LL++           + P+H A  + + ++   LLD   ++     +G+ P+H A      E+  +L  ++ +
Sbjct:  464 VVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS 573          

HSP 10 Score: 45.0542 bits (105), Expect = 4.653e-6
Identity = 24/107 (22.43%), Postives = 49/107 (45.79%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQ L++   +    T     P+H A  + + ++   LL+    +     + + P+H A      E+ +LLL ++ + +     G  P+H A   +N ++  +L D+
Sbjct:  497 IVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 603          

HSP 11 Score: 44.669 bits (104), Expect = 5.029e-6
Identity = 26/111 (23.42%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KT+  L P+H      + ++  +LL+R+  I  +      P+H A    +   V LLL  N  ++   N     +H A    + ++ ++L D+  +
Sbjct:  265 NMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKAS 375          

HSP 12 Score: 44.669 bits (104), Expect = 5.434e-6
Identity = 24/113 (21.24%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL++ + + +   K  L P+H A   +N ++  LLL++           + P+H A  K   ++   LL+   +      +G   +H A    + +++ +L  RN N+
Sbjct:  561 LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANV 673          
BLAST of SMED30005565 vs. UniProt/SwissProt
Match: sp|Q9J504|V231_FOWPN (Putative ankyrin repeat protein FPV231 OS=Fowlpox virus (strain NVSL) OX=928301 GN=FPV231 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 4.729e-13
Identity = 33/111 (29.73%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            +I ++L+++   I+ + K++  P++ AI   N E+ +L L R  D    D      +H AI   N   V LLL+   +    DN G+  +H+AI  +N EI +ML     +
Sbjct:    6 TISKMLLDYGARIDSRNKEECLPLNHAIATNNKELTSLFLARGADTNIVDKYNRSVLHKAIGNNNITSVKLLLNHGIDYNLRDNHGYTALHYAITLQNREITDMLLSSGAD 116          
BLAST of SMED30005565 vs. UniProt/SwissProt
Match: sp|B0G124|Y9043_DICDI (Ankyrin repeat-containing protein DDB_G0279043 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0279043 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.749e-13
Identity = 36/112 (32.14%), Postives = 55/112 (49.11%), Query Frame = -2
Query:    7 FSIVQLLIEH-NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNC 339
            F  V+ +I+  N N+E      L P+HFA    N  I   LL++  DI  E+N    PI  A+ +   E V  L+ R  N+  GD     P+H A++ E+ E++ +L D   
Sbjct:   48 FEDVKKMIDSGNVNLEEGDFGGLHPLHFAARMGNIAIGQYLLDKGVDINAENNYGSTPILEAVRRGEVEFVKFLISRGANLSIGDIDDNTPLHLAVMCEDGELIPILLDAGS 159          
BLAST of SMED30005565 vs. UniProt/SwissProt
Match: sp|Q8BZ25|ANKK1_MOUSE (Ankyrin repeat and protein kinase domain-containing protein 1 OS=Mus musculus OX=10090 GN=Ankk1 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.055e-12
Identity = 29/110 (26.36%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL+ H+ +++C+T     P+  A   +  ++  LLL    D    D   W P+H+A    +     LLLD    +   ++ GW P+H A  N    +  +L  R  ++
Sbjct:  386 VRLLLSHDVDVDCQTASGYTPLLIATQDQQPDLCALLLAHGADTNLADEDGWAPLHFAAQNGDDHTARLLLDHGALVNAREHEGWTPLHLAAQNNFENVARLLVSRQADL 495          

HSP 2 Score: 55.4546 bits (132), Expect = 5.553e-9
Identity = 25/93 (26.88%), Postives = 49/93 (52.69%), Query Frame = -2
Query:   55 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAI 333
            I ++L+ +  ++E +T+Q   P+H A  K + EI + L + + D++   + +W P+H A  +    ++  LL    N    +  GW P+H A+
Sbjct:  583 IFKMLLRYGASLELRTQQGWTPLHLATYKGHLEIIHQLAKSHVDLDALGSMQWTPLHLAAFQGEEGVMLALLQCGANPNAAEQSGWTPLHLAV 675          

HSP 3 Score: 52.373 bits (124), Expect = 7.281e-8
Identity = 23/90 (25.56%), Postives = 43/90 (47.78%), Query Frame = -2
Query:   16 KQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 285
            + ++ P+HF +   + E   LLL  + D++C+    + P+  A   +  ++  LLL    +    D  GW P+H+A  N +     +L D
Sbjct:  368 ENRVTPLHFLVAGGSLEQVRLLLSHDVDVDCQTASGYTPLLIATQDQQPDLCALLLAHGADTNLADEDGWAPLHFAAQNGDDHTARLLLD 457          
BLAST of SMED30005565 vs. UniProt/SwissProt
Match: sp|P0DJE3|LATA_LATHE (Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=256737 PE=1 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 3.354e-12
Identity = 30/107 (28.04%), Postives = 55/107 (51.40%), Query Frame = -2
Query:    4 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 324
            LL   N N+  K    + P+H A+M+   ++ +L+     +IE + ++++ P+H A   K  E++ +LLD++ N E   N G  P+H A     S+   +L +   N
Sbjct:  715 LLRSSNVNVNLKALGGITPLHLAVMQGRKQVLSLMFNIGVNIEQQTDEKYTPLHLAAMSKYPELIQILLDQDSNFEAKTNSGATPLHLATFKGKSQAALILLNNEVN 821          

HSP 2 Score: 55.8398 bits (133), Expect = 4.272e-9
Identity = 29/92 (31.52%), Postives = 47/92 (51.09%), Query Frame = -2
Query:   58 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 333
            ++ L+     NIE +T +K  P+H A M +  E+  +LL+++ + E + N    P+H A  K  S+   +LL+   N    D  G  PIH A
Sbjct:  745 VLSLMFNIGVNIEQQTDEKYTPLHLAAMSKYPELIQILLDQDSNFEAKTNSGATPLHLATFKGKSQAALILLNNEVNWRDTDENGQMPIHGA 836          
BLAST of SMED30005565 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.369e-12
Identity = 33/108 (30.56%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N+ MV LLLDR   I+     G  P+H    + + +++EML DR+  I
Sbjct:  203 LLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPI 310          

HSP 2 Score: 53.5286 bits (127), Expect = 2.844e-8
Identity = 29/111 (26.13%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   +I   LLE   D      Q    +H A  + + +MV+LLL RN N+   +  G  P+H A   +   + E+L ++  ++
Sbjct:  596 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHV 706          

HSP 3 Score: 52.7582 bits (125), Expect = 5.346e-8
Identity = 24/110 (21.82%), Postives = 51/110 (46.36%), Query Frame = -2
Query:    4 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 333
            +V LL+  N N+    K  L P+H A  ++   +  +L+ +   ++ +    + P+H   +  N ++VN LL  +  +      G+  +H A    ++ I+ +L   N +
Sbjct:  662 MVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771          

HSP 4 Score: 51.9878 bits (123), Expect = 1.232e-7
Identity = 25/109 (22.94%), Postives = 51/109 (46.79%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H      + ++V +LLDR+  I      G  P+H A   ++   +++L   N
Sbjct:  232 NVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN 340          

HSP 5 Score: 50.8322 bits (120), Expect = 2.455e-7
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N+ ++++L DR   I
Sbjct:  171 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKI 277          

HSP 6 Score: 48.521 bits (114), Expect = 2.076e-6
Identity = 27/111 (24.32%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++ +  I  KTK  L P+H A   ++     LLL+ N  ++   N     +H A +  + ++  +LLD+  +       G+ P+H A       ++E+L     +I
Sbjct:  299 VVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASI 409          

HSP 7 Score: 47.3654 bits (111), Expect = 4.055e-6
Identity = 25/106 (23.58%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +   L++H  ++   TK+   P+H A      E+ +LLL+++   +        P+H A +  N ++  LLLD+  +       G+ P+H A      +I   L +
Sbjct:  530 VAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLE 635          

HSP 8 Score: 46.595 bits (109), Expect = 8.727e-6
Identity = 22/111 (19.82%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q +  +H A  + + ++ +LLL RN ++   +     P+H A  +    +  +L+++  +++     G+ P+H      N +I+  L   +  +
Sbjct:  629 IATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKV 739          
BLAST of SMED30005565 vs. TrEMBL
Match: A0A162PBA5 (Ankyrin repeat protein OS=Colletotrichum incanum OX=1573173 GN=CI238_12255 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.384e-22
Identity = 39/112 (34.82%), Postives = 75/112 (66.96%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            SIV+LL+E   ++E K  +   P+H+A+++E+  I  LL+E   D+E +DN+ + P+HWA+ +++  +V LL++   ++E  DN G+ P+HWA+I E+  I+++L +   ++
Sbjct: 1261 SIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADM 1372          

HSP 2 Score: 92.4337 bits (228), Expect = 1.967e-19
Identity = 38/113 (33.63%), Postives = 75/113 (66.37%), Query Frame = -2
Query:    1 SIVQLLI-EHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            SIV+LL  E +C+ + +  +   P+H+A+++E+  I  LL+E   D+E +DN+ + P+HWA+ +++  +V LL++   ++E  DN G+ P+HWA+I E+  I+++L +   ++
Sbjct: 1227 SIVKLLAGELSCDKDARDNEGWTPLHWAVIEEHESIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADM 1339          

HSP 3 Score: 79.7221 bits (195), Expect = 6.673e-15
Identity = 40/115 (34.78%), Postives = 67/115 (58.26%), Query Frame = -2
Query:    1 SIVQLLI-EHNCNIECKTKQKLRPIHFAIMKENSEIFNLLL-ERNCDIECEDNQRWRPIHWAINKKNSEMVNLLL-DRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            SIV+LL  E +C+ + K      P+H A+ + +  I  LL  E +CD +  DN+   P+HWA+ K    +V LL  + +C+ +  DN GW P+HWA+I E+  I+++L +   ++
Sbjct: 1159 SIVKLLAGELSCDKDTKDDSGWTPLHHAVREGHESIVKLLAGELSCDKDARDNEGRTPLHWAVTKGYESIVKLLAGELSCDKDARDNEGWTPLHWAVIEEHESIVKLLVEAGADM 1273          

HSP 4 Score: 77.0258 bits (188), Expect = 5.336e-14
Identity = 32/90 (35.56%), Postives = 59/90 (65.56%), Query Frame = -2
Query:   67 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPI 336
            SIV+LL+E   ++E K  +   P+H+A+++E+  I  LL+E   D+E +DN+ + P+HWA+ +++  +V LL++   ++E  DN G  P+
Sbjct: 1294 SIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADMEAKDNEGYTPLHWAVIEEHESIVKLLVEAGADMEAKDNDGNTPL 1383          
BLAST of SMED30005565 vs. TrEMBL
Match: A0A2P1P6S3 (ANK_REP_REGION domain-containing protein OS=Candidatus Phycorickettsia trachydisci OX=2115978 GN=phytr_90 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.720e-19
Identity = 46/110 (41.82%), Postives = 65/110 (59.09%), Query Frame = -2
Query:    1 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIEC-EDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 327
            QLLI +  NI    K K +PIHFA  +   EI +LLL +   IEC E+N  + P+HWA NK ++++++ L+ R  NIE  DN+   P+HWA   +  E L+ L D   NI
Sbjct: 1634 QLLISNGANIHTFNKAKYQPIHFAASRGYPEIIDLLLNQGAKIECKEENNYFTPLHWAANKGHAKIISTLVKRGANIESVDNKAKTPLHWAAGTDQLEALQTLLDLGANI 1743          

HSP 2 Score: 67.0106 bits (162), Expect = 1.619e-10
Identity = 36/112 (32.14%), Postives = 64/112 (57.14%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECG-DNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I+++LI++   IE KT+     +  AI K N E   LL+    +I   +  +++PIH+A ++   E+++LLL++   IEC  +N  + P+HWA    +++I+  L  R  NI
Sbjct: 1599 ILKILIKYKAFIEAKTEGDWTALLVAIEKGNLEATQLLISNGANIHTFNKAKYQPIHFAASRGYPEIIDLLLNQGAKIECKEENNYFTPLHWAANKGHAKIISTLVKRGANI 1710          

HSP 3 Score: 53.5286 bits (127), Expect = 9.376e-6
Identity = 29/109 (26.61%), Postives = 52/109 (47.71%), Query Frame = -2
Query:    1 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 327
            ++L+ +  NI    K     +H A +  N++I  +L++    IE +    W  +  AI K N E   LL+    NI   +   ++PIH+A      EI+++L ++   I
Sbjct: 1568 EVLLSNGANIAATLKVNETSLHIAALNNNTDILKILIKYKAFIEAKTEGDWTALLVAIEKGNLEATQLLISNGANIHTFNKAKYQPIHFAASRGYPEIIDLLLNQGAKI 1676          
BLAST of SMED30005565 vs. TrEMBL
Match: A0A072NUD0 (ANK_REP_REGION domain-containing protein OS=Exophiala aquamarina CBS 119918 OX=1182545 GN=A1O9_12954 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.435e-19
Identity = 36/111 (32.43%), Postives = 62/111 (55.86%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    +ECK +     + +A+   +  +  LLL+R  D+EC+D     P+ WA+   N  +V LLLDR   +EC D  GW P+ WA+   N  ++++L D+   
Sbjct:   72 TVVRLLLDRGAGLECKDRHGWTLLSWAVEYGDEALVQLLLDRGADLECKDRDGRTPLLWAVEYGNEALVQLLLDRGAGLECKDRHGWTPLLWAVEYGNEAVVQLLLDKGAT 182          

HSP 2 Score: 82.4185 bits (202), Expect = 1.111e-16
Identity = 35/111 (31.53%), Postives = 60/111 (54.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +VQ L++   ++ECK +    P+  A +     +  LLL+R   +EC+D   W  + WA+   +  +V LLLDR  ++EC D  G  P+ WA+   N  ++++L DR   +
Sbjct:   40 VVQQLLDKGADLECKDRDGRTPLWRAAINGCETVVRLLLDRGAGLECKDRHGWTLLSWAVEYGDEALVQLLLDRGADLECKDRDGRTPLLWAVEYGNEALVQLLLDRGAGL 150          

HSP 3 Score: 75.485 bits (184), Expect = 4.368e-14
Identity = 34/112 (30.36%), Postives = 61/112 (54.46%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++V+LL+E   ++ECK +    P+ +A   +N  +   LL++  D+EC+D     P+  A       +V LLLDR   +EC D  GW  + WA+   +  ++++L DR  ++
Sbjct:    6 TVVRLLLERRVDLECKDRDGRTPLLWAAHYQNDVVVQQLLDKGADLECKDRDGRTPLWRAAINGCETVVRLLLDRGAGLECKDRHGWTLLSWAVEYGDEALVQLLLDRGADL 117          

HSP 4 Score: 73.9442 bits (180), Expect = 1.642e-13
Identity = 33/108 (30.56%), Postives = 60/108 (55.56%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            ++VQLL++   ++ECK +    P+ +A+   N  +  LLL+R   +EC+D   W P+ WA+   N  +V LLLD+        + GW  + WA ++ + ++ + L +R
Sbjct:  105 ALVQLLLDRGADLECKDRDGRTPLLWAVEYGNEALVQLLLDRGAGLECKDRHGWTPLLWAVEYGNEAVVQLLLDKGAT-----HDGWTLLWWATMHGHKDVAKKLQER 207          

HSP 5 Score: 59.6918 bits (143), Expect = 3.797e-8
Identity = 29/84 (34.52%), Postives = 44/84 (52.38%), Query Frame = -2
Query:    1 MKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 252
            M     +  LLLER  D+EC+D     P+ WA + +N  +V  LLD+  ++EC D  G  P+  A IN    ++ +L DR   +
Sbjct:    1 MNGRETVVRLLLERRVDLECKDRDGRTPLLWAAHYQNDVVVQQLLDKGADLECKDRDGRTPLWRAAINGCETVVRLLLDRGAGL 84          
BLAST of SMED30005565 vs. TrEMBL
Match: A0A3G5A969 (ANK_REP_REGION domain-containing protein (Fragment) OS=Homavirus sp. OX=2487769 GN=Homavirus39_1 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.143e-18
Identity = 47/111 (42.34%), Postives = 67/111 (60.36%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            IV+ LI+ N ++EC+T  K RPIH A    N EIF  L+++N +IECE   R +PIH      N EM   L+D+N N+ECG      PIH A  N  ++I++ L D+N ++
Sbjct:   26 IVKYLIDKNVSLECETLCKHRPIHIACRNSNIEIFKYLMDKNVNIECEIFPRMKPIHIVCRNGNIEMFKYLMDKNVNLECGTIDHETPIHIACENNYTDIVKDLIDKNVDL 136          

HSP 2 Score: 79.7221 bits (195), Expect = 7.815e-16
Identity = 42/103 (40.78%), Postives = 60/103 (58.25%), Query Frame = -2
Query:    1 NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 309
            N ++EC+TK K RPIH A    N++I   L+++N  +ECE   + RPIH A    N E+   L+D+N NIEC      +PIH    N N E+ + L D+N N+
Sbjct:    1 NVSLECETKDKDRPIHLACKNNNTDIVKYLIDKNVSLECETLCKHRPIHIACRNSNIEIFKYLMDKNVNIECEIFPRMKPIHIVCRNGNIEMFKYLMDKNVNL 103          

HSP 3 Score: 64.3142 bits (155), Expect = 5.469e-10
Identity = 37/123 (30.08%), Postives = 62/123 (50.41%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDN-------RG-----WRPIHWAIINENSEILEMLFDRNCNI 333
            I + L++ N NIEC+   +++PIH      N E+F  L+++N ++EC       PIH A     +++V  L+D+N ++EC D        +G     WRPIH+     +   +  L D   N+
Sbjct:   59 IFKYLMDKNVNIECEIFPRMKPIHIVCRNGNIEMFKYLMDKNVNLECGTIDHETPIHIACENNYTDIVKDLIDKNVDLECEDGYKIVSFKKGEATYKWRPIHYISKFGDINTIMYLIDHGVNL 181          
BLAST of SMED30005565 vs. TrEMBL
Match: A0A428NB56 (ANK_REP_REGION domain-containing protein (Fragment) OS=Fusarium sp. AF-6 OX=1325737 GN=CEP53_015273 PE=4 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.637e-18
Identity = 41/112 (36.61%), Postives = 66/112 (58.93%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            + VQLL++    IE K ++   P+ +A++  N  I  LLL R+ DIE +D +   P+ WA+  +N   + LLL+R+ +IE  D RG   + WAI + +  I+++L DR  NI
Sbjct:  894 ATVQLLLDRGAEIEAKDREGWTPLFWAVVHRNEAIMRLLLSRDADIEAKDKEGRTPLFWAVVHRNEAAMRLLLNRDADIEVKDQRGQTLLFWAIQSRHKAIIQLLIDRGANI 1005          

HSP 2 Score: 88.9669 bits (219), Expect = 3.495e-18
Identity = 40/112 (35.71%), Postives = 66/112 (58.93%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            +I++LL+  + +IE K K+   P+ +A++  N     LLL R+ DIE +D +    + WAI  ++  ++ LL+DR  NIE  D  GW P+ +A  N +  I+++L DR  NI
Sbjct:  927 AIMRLLLSRDADIEAKDKEGRTPLFWAVVHRNEAAMRLLLNRDADIEVKDQRGQTLLFWAIQSRHKAIIQLLIDRGANIEAKDEDGWTPLLYAAANGHEAIMQLLIDRGANI 1038          

HSP 3 Score: 82.4185 bits (202), Expect = 6.891e-16
Identity = 34/112 (30.36%), Postives = 64/112 (57.14%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++++LL++    IE K ++   P+  A    +     LLL+R  +IE +D + W P+ WA+  +N  ++ LLL R+ +IE  D  G  P+ WA+++ N   + +L +R+ +I
Sbjct:  861 AVMRLLLDRGAEIEAKDQRGQTPLVLATSNGDKATVQLLLDRGAEIEAKDREGWTPLFWAVVHRNEAIMRLLLSRDADIEAKDKEGRTPLFWAVVHRNEAAMRLLLNRDADI 972          

HSP 4 Score: 76.6406 bits (187), Expect = 6.561e-14
Identity = 37/108 (34.26%), Postives = 59/108 (54.63%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            +I+QLLI+   NIE K +    P+ +A    +  I  LL++R  +IE +D     P+  A +  +   V LL+D+  NIE  D  GW P+ +A  N +  I+++L DR
Sbjct:  993 AIIQLLIDRGANIEAKDEDGWTPLLYAAANGHEAIMQLLIDRGANIEAKDKDGQTPLVLATSNGDEAAVQLLIDQGANIEAKDEDGWTPLSYAAANGHEAIMQLLIDR 1100          

HSP 5 Score: 73.1738 bits (178), Expect = 1.321e-12
Identity = 34/107 (31.78%), Postives = 57/107 (53.27%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V+LL+E   ++E K ++   P+ +A    +  +  LLLER  D+E +D +   P+ WA   +    V LLLDR   IE  +  G  P+ +A  N +   +++L DR
Sbjct:  705 VVRLLLERGADVEAKDEEGQTPLSYAAENGHEAVMRLLLERGADVEAKDEEGRTPLFWAAANRREAAVQLLLDRGARIEAANKGGQTPLSYAAANGDKADVQLLLDR 811          

HSP 6 Score: 66.6254 bits (161), Expect = 2.412e-10
Identity = 33/110 (30.00%), Postives = 59/110 (53.64%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            ++LL+  + +IE K ++    + +AI   +  I  LL++R  +IE +D   W P+ +A    +  ++ LL+DR  NIE  D  G  P+  A  N +   +++L D+  NI
Sbjct:  962 MRLLLNRDADIEVKDQRGQTLLFWAIQSRHKAIIQLLIDRGANIEAKDEDGWTPLLYAAANGHEAIMQLLIDRGANIEAKDKDGQTPLVLATSNGDEAAVQLLIDQGANI 1071          

HSP 7 Score: 64.6994 bits (156), Expect = 1.109e-9
Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++V LL+E   +      +    + +A +  +  +  LLLER  D+E +D +   P+ +A    +  ++ LLL+R  ++E  D  G  P+ WA  N     +++L DR   I
Sbjct:  671 TVVGLLLERGTDFNAADNEDRTSLAYAAVNGHEPVVRLLLERGADVEAKDEEGQTPLSYAAENGHEAVMRLLLERGADVEAKDEEGRTPLFWAAANRREAAVQLLLDRGARI 782          

HSP 8 Score: 64.3142 bits (155), Expect = 1.413e-9
Identity = 34/112 (30.36%), Postives = 55/112 (49.11%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            +I+QLLI+   NIE K K    P+  A    +     LL+++  +IE +D   W P+ +A    +  ++ LL+DR    E  D  G  P   A  N +  I+ +L DR  ++
Sbjct: 1026 AIMQLLIDRGANIEAKDKDGQTPLVLATSNGDEAAVQLLIDQGANIEAKDEDGWTPLSYAAANGHEAIMQLLIDRGAYTEMIDEEGQAPSLLAASNGDEAIVRLLLDRGADV 1137          

HSP 9 Score: 55.0694 bits (131), Expect = 2.504e-6
Identity = 28/101 (27.72%), Postives = 50/101 (49.50%), Query Frame = -2
Query:    1 NIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 303
            NI+ K      P+ +A  K +  +  LLLER  D    DN+    + +A    +  +V LLL+R  ++E  D  G  P+ +A  N +  ++ +L +R  ++
Sbjct:  649 NIDAKDWDGRTPLFWAAAKGHKTVVGLLLERGTDFNAADNEDRTSLAYAAVNGHEPVVRLLLERGADVEAKDEEGQTPLSYAAENGHEAVMRLLLERGADV 749          
BLAST of SMED30005565 vs. Ensembl Cavefish
Match: ankk1 (ankyrin repeat and kinase domain containing 1 [Source:NCBI gene;Acc:103036669])

HSP 1 Score: 64.6994 bits (156), Expect = 4.753e-13
Identity = 28/106 (26.42%), Postives = 54/106 (50.94%), Query Frame = -2
Query:   13 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 330
            VQ+++     + C++ +   P+  A+++   +I  LL++   D+   D+  W P+H+A+   N   V +LLD     +  D  GW P+H A  N +  ++ +L  R
Sbjct:  374 VQMVLNMGALVNCQSVRGYTPLIVALLRTYYDICALLIDHGADVNLGDHDLWTPLHFAVQAGNDRAVRMLLDHKATADAKDKDGWTPLHLAAQNGHEGVVRLLLPR 479          

HSP 2 Score: 54.299 bits (129), Expect = 1.980e-9
Identity = 28/92 (30.43%), Postives = 43/92 (46.74%), Query Frame = -2
Query:   58 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 333
            I +LL+ H  N    T QK  P+H A +K + E   +L E    ++      W P+H A +    E+V +LL    N    ++  W P+H A
Sbjct:  572 ICRLLMNHGANPNFTTNQKWTPMHLAALKGHPETVLILEENKGSMDARGQGGWTPLHLACHHMQEEVVAVLLTAGANPNLKEDGSWTPLHLA 663          

HSP 3 Score: 51.2174 bits (121), Expect = 2.265e-8
Identity = 27/112 (24.11%), Postives = 51/112 (45.54%), Query Frame = -2
Query:    4 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 339
            F + +LL+    ++          +HFA ++  + I  LL+    +     NQ+W P+H A  K + E V +L +   +++     GW P+H A  +   E++ +L     N
Sbjct:  537 FRVARLLVTKGADVNRVNGSNYSALHFAALRGCTGICRLLMNHGANPNFTTNQKWTPMHLAALKGHPETVLILEENKGSMDARGQGGWTPLHLACHHMQEEVVAVLLTAGAN 648          

HSP 4 Score: 47.7506 bits (112), Expect = 4.126e-7
Identity = 28/113 (24.78%), Postives = 50/113 (44.25%), Query Frame = -2
Query:    4 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNI-ECGDNRGWRPIHWAIINENSEILEMLFDRNCN 339
            + I  LLI+H  ++         P+HFA+   N     +LL+     + +D   W P+H A    +  +V LLL R  ++ E  +  G   +H A +     I ++L  +  +
Sbjct:  404 YDICALLIDHGADVNLGDHDLWTPLHFAVQAGNDRAVRMLLDHKATADAKDKDGWTPLHLAAQNGHEGVVRLLLPRLVSVDEQEETSGRTSLHMACMYGYLNIAKLLLAKGAD 516          
BLAST of SMED30005565 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 63.929 bits (154), Expect = 9.367e-13
Identity = 33/108 (30.56%), Postives = 55/108 (50.93%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++ +      P+H A  + NS MV LLL+R   I+     G  P+H    + + +++EML DR   I
Sbjct:  167 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 274          

HSP 2 Score: 54.299 bits (129), Expect = 2.015e-9
Identity = 27/106 (25.47%), Postives = 50/106 (47.17%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            ++  LL+     ++ K +  + P+H A  + NS +  LLLER   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L 
Sbjct:  196 NVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL 301          

HSP 3 Score: 53.5286 bits (127), Expect = 3.180e-9
Identity = 30/111 (27.03%), Postives = 47/111 (42.34%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   EI   LLE   D      Q   P+H A  + N +MV LL+ R+  I   +  G  P+H A   +   + E+L +    +
Sbjct:  560 VALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATV 670          

HSP 4 Score: 49.6766 bits (117), Expect = 6.903e-8
Identity = 30/111 (27.03%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    NS ++++L +R   I
Sbjct:  135 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARI 241          

HSP 5 Score: 48.1358 bits (113), Expect = 2.988e-7
Identity = 25/107 (23.36%), Postives = 51/107 (47.66%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            I+Q L++H    +  T     P+H A  + + ++ ++LL++   +     + + P+H A      E+ NLLL +    +     G  P+H A   +N ++  +L D+
Sbjct:  461 IIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQ 567          

HSP 6 Score: 47.7506 bits (112), Expect = 3.735e-7
Identity = 26/106 (24.53%), Postives = 47/106 (44.34%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +  +L++   ++   TK+   P+H A      E+ NLLL++    +        P+H A +  N ++  LLLD+  +       G+ P+H A      EI   L +
Sbjct:  494 VASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLE 599          

HSP 7 Score: 45.4394 bits (106), Expect = 2.141e-6
Identity = 23/111 (20.72%), Postives = 49/111 (44.14%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I   L+E+  +    T+Q + P+H A  + N ++  LL+ R+  I   +     P+H A  +    +  +L++    ++     G+ P+H A    N +++  L      +
Sbjct:  593 IATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKV 703          

HSP 8 Score: 45.4394 bits (106), Expect = 2.183e-6
Identity = 26/111 (23.42%), Postives = 48/111 (43.24%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++     +   K  L P+H A   +N ++  LLL++           + P+H A  K   E+   LL+   +      +G  P+H A    N +++ +L  R+  I
Sbjct:  527 VANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATI 637          

HSP 9 Score: 44.669 bits (104), Expect = 3.906e-6
Identity = 25/111 (22.52%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL     ++   N     +H A +  + ++  +++D+  N       G+ P+H A      +++E+L     +I
Sbjct:  263 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 373          

HSP 10 Score: 44.669 bits (104), Expect = 5.124e-6
Identity = 27/111 (24.32%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL+E    I+ KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL     ++   N     +H A    + ++ +++ D+  N
Sbjct:  229 NMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKAN 339          

HSP 11 Score: 44.2838 bits (103), Expect = 5.756e-6
Identity = 22/107 (20.56%), Postives = 51/107 (47.66%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V+ L+++   ++ K+K    P+H +      EI   LL+     +      + P+H A  + + ++ ++LLD+  ++     +G+ P+H A      E+  +L  +
Sbjct:  428 VVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQK 534          
BLAST of SMED30005565 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910])

HSP 1 Score: 63.1586 bits (152), Expect = 1.458e-12
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +++LL++HN ++E + K   R +H A   +   +  +L     D+   + +R  P+H A+NK + ++V  LLD  C+    D+ G  P+H AI  +  ++L +L +   ++
Sbjct:  476 VLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADV 586          

HSP 2 Score: 57.3806 bits (137), Expect = 1.457e-10
Identity = 26/108 (24.07%), Postives = 60/108 (55.56%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            S++++L     ++  + K++  P+H A+ K + ++   LL+  C    +D++   P+H AI+KK  +M+++LL+   ++   +N G+  +H A +  N   + +L  +
Sbjct:  508 SVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSK 615          
BLAST of SMED30005565 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910])

HSP 1 Score: 63.1586 bits (152), Expect = 1.499e-12
Identity = 30/111 (27.03%), Postives = 60/111 (54.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +++LL++HN ++E + K   R +H A   +   +  +L     D+   + +R  P+H A+NK + ++V  LLD  C+    D+ G  P+H AI  +  ++L +L +   ++
Sbjct:  479 VLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADV 589          

HSP 2 Score: 57.3806 bits (137), Expect = 1.513e-10
Identity = 26/108 (24.07%), Postives = 60/108 (55.56%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            S++++L     ++  + K++  P+H A+ K + ++   LL+  C    +D++   P+H AI+KK  +M+++LL+   ++   +N G+  +H A +  N   + +L  +
Sbjct:  511 SVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSK 618          
BLAST of SMED30005565 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 63.1586 bits (152), Expect = 1.508e-12
Identity = 33/108 (30.56%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + N  MV LLLDR   I+     G  P+H    + + +++EML DR   I
Sbjct:  222 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPI 329          

HSP 2 Score: 57.7658 bits (138), Expect = 1.205e-10
Identity = 28/105 (26.67%), Postives = 50/105 (47.62%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+  N N+    K  L P+H A  ++   +  +LL    +++      + P+H A +  N +MV+ LL+    I      G+ P+H A    ++ I+ ML 
Sbjct:  681 LVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLL 785          

HSP 3 Score: 57.3806 bits (137), Expect = 1.677e-10
Identity = 30/111 (27.03%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++H  +     K    P+H A  K   EI   LLE   +      Q   P+H A  + + ++V+LLL +N N+  G+  G  P+H A   +   + E+L +    +
Sbjct:  615 VALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEV 725          

HSP 4 Score: 49.6766 bits (117), Expect = 7.829e-8
Identity = 24/109 (22.02%), Postives = 49/109 (44.95%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + N  +  LLL+R   I+ +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   +
Sbjct:  251 NVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHD 359          

HSP 5 Score: 49.6766 bits (117), Expect = 9.056e-8
Identity = 30/111 (27.03%), Postives = 56/111 (50.45%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    N  ++++L DR   I
Sbjct:  190 VVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKI 296          

HSP 6 Score: 48.1358 bits (113), Expect = 2.533e-7
Identity = 28/106 (26.42%), Postives = 45/106 (42.45%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            IVQLL++        T     P+H A+ + + E  +LLLE    +     + + P+H A      E   LLL +    +     G  P+H A   +N  +  +L D
Sbjct:  516 IVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLD 621          

HSP 7 Score: 47.3654 bits (111), Expect = 5.779e-7
Identity = 24/107 (22.43%), Postives = 50/107 (46.73%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V+ L+++   ++ K K     +H A     +EI  LLL++           + P+H A+ + + E  +LLL+   ++     +G+ P+H A      E  ++L  +
Sbjct:  483 VVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQK 589          

HSP 8 Score: 46.9802 bits (110), Expect = 6.815e-7
Identity = 23/107 (21.50%), Postives = 49/107 (45.79%), Query Frame = -2
Query:    1 LIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 321
            L+E        T+Q + P+H A  + + ++ +LLL +N ++   +     P+H A  +    +  +LL+    ++     G+ P+H A    N +++  L +    I
Sbjct:  652 LLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKI 758          

HSP 9 Score: 46.2098 bits (108), Expect = 1.232e-6
Identity = 28/103 (27.18%), Postives = 43/103 (41.75%), Query Frame = -2
Query:   16 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 324
            LL+EH  ++   TK+   P+H A      E   LLL++    +        P+H A +  N  +  LLLD   +       G+ P+H A      EI   L +
Sbjct:  552 LLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLE 654          

HSP 10 Score: 45.4394 bits (106), Expect = 2.183e-6
Identity = 24/111 (21.62%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ +  ++   N     +H A +  + ++  +++D+  N       G+ P+H A      +++E+L     ++
Sbjct:  318 VVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASL 428          

HSP 11 Score: 44.2838 bits (103), Expect = 6.721e-6
Identity = 26/111 (23.42%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    I+ KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  +  ++   N     +H A    + ++ +++ D+  N
Sbjct:  284 NMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKAN 394          
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.7734 bits (151), Expect = 4.646e-13
Identity = 33/108 (30.56%), Postives = 53/108 (49.07%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++H  N +  +K    P+H A    N  +  LLL     ++        P+H A  + N+ MV LLLDR   I+     G  P+H A  + + +++EML +R+  I
Sbjct:  190 LLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPI 297          

HSP 2 Score: 51.2174 bits (121), Expect = 5.057e-9
Identity = 25/112 (22.32%), Postives = 53/112 (47.32%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H A    + +++ +LL+R+  I      G  P+H A   E+   +++L  R   +
Sbjct:  219 NVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV 330          

HSP 3 Score: 50.0618 bits (118), Expect = 1.121e-8
Identity = 30/111 (27.03%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            ++++L+E +  I  KTK  L P+H A   E+     LLL R   ++        P+H A +  +  +  LLLDR          G+ P+H A      +++E+L     +I
Sbjct:  286 VIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASI 396          

HSP 4 Score: 49.2914 bits (116), Expect = 2.066e-8
Identity = 29/108 (26.85%), Postives = 53/108 (49.07%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            ++V+LL++    I+ KT+  L P+H A    + ++  +LLER+  I  +      P+H A   ++   V LLL R   ++        P+H A    +  + ++L DR
Sbjct:  252 NMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDR 359          

HSP 5 Score: 46.2098 bits (108), Expect = 2.383e-7
Identity = 25/109 (22.94%), Postives = 52/109 (47.71%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              +V+ L++    +  K ++   P+H A     +EI  LLL+R  D          P+++A  + + ++ +LLL+   +I     +G+ P+H A      E+  +L ++
Sbjct:  449 LEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEK 557          

HSP 6 Score: 46.2098 bits (108), Expect = 2.783e-7
Identity = 29/111 (26.13%), Postives = 53/111 (47.75%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E     + K K +L  +H A  K+++    LLL+   + +      + P+H A +  N  +  LLL+    ++     G  P+H A    N+ ++++L DR   I
Sbjct:  158 VVGLLLES----DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKI 264          

HSP 7 Score: 45.0542 bits (105), Expect = 6.225e-7
Identity = 27/111 (24.32%), Postives = 48/111 (43.24%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   E+  +LLE   +      Q   P+H A  + +++MV LLL R+ +I      G   +H A   +   + E+L     ++
Sbjct:  585 VALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADV 695          

HSP 8 Score: 45.0542 bits (105), Expect = 7.269e-7
Identity = 25/105 (23.81%), Postives = 47/105 (44.76%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+  + +I   TK  L  +H A  ++   +  +L +   D+       + P+H A +  N +MVN LL     +      G+ P+H A    ++ ++  L 
Sbjct:  651 MVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755          

HSP 9 Score: 44.669 bits (104), Expect = 1.011e-6
Identity = 27/107 (25.23%), Postives = 47/107 (43.93%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +++LL++H  +I   T+  L PIH A    +  +  LLL+        + +   P+H A      E+V  LL     +         P+H A     +EI+++L  R
Sbjct:  385 VMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQR 491          
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 2.410e-11
Identity = 33/111 (29.73%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 EHNCNIECK------TKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 315
            EHN ++  K      T+    P+H A    N  +  LLL     ++        P+H A  + N+ MV LLLDR   I+     G  P+H A  + + +++EML +R+  I
Sbjct:  194 EHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPI 304          

HSP 2 Score: 51.2174 bits (121), Expect = 5.080e-9
Identity = 25/112 (22.32%), Postives = 53/112 (47.32%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++  LL+     ++   +  + P+H A  + N+ +  LLL+R   I+ +      P+H A    + +++ +LL+R+  I      G  P+H A   E+   +++L  R   +
Sbjct:  226 NVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV 337          

HSP 3 Score: 50.0618 bits (118), Expect = 1.126e-8
Identity = 30/111 (27.03%), Postives = 51/111 (45.95%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            ++++L+E +  I  KTK  L P+H A   E+     LLL R   ++        P+H A +  +  +  LLLDR          G+ P+H A      +++E+L     +I
Sbjct:  293 VIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASI 403          

HSP 4 Score: 49.2914 bits (116), Expect = 2.076e-8
Identity = 29/108 (26.85%), Postives = 53/108 (49.07%), Query Frame = -2
Query:   13 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 336
            ++V+LL++    I+ KT+  L P+H A    + ++  +LLER+  I  +      P+H A   ++   V LLL R   ++        P+H A    +  + ++L DR
Sbjct:  259 NMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDR 366          

HSP 5 Score: 48.521 bits (114), Expect = 4.257e-8
Identity = 28/107 (26.17%), Postives = 53/107 (49.53%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            IVQLL++   +    T     P++FA  + + ++ +LLLE   DI     + + P+H A      E+  LLL++  + +     G  P+H A   ++ ++  +L D+
Sbjct:  491 IVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDK 597          

HSP 6 Score: 46.595 bits (109), Expect = 2.110e-7
Identity = 27/106 (25.47%), Postives = 50/106 (47.17%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +  LL+E+  +I   TK+   P+H A      E+  LLLE+    + +      P+H A +  + ++  LLLD+  +       G+ P+H A      E+ ++L +
Sbjct:  524 VASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLE 629          

HSP 7 Score: 46.2098 bits (108), Expect = 2.394e-7
Identity = 25/109 (22.94%), Postives = 52/109 (47.71%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              +V+ L++    +  K ++   P+H A     +EI  LLL+R  D          P+++A  + + ++ +LLL+   +I     +G+ P+H A      E+  +L ++
Sbjct:  456 LEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEK 564          

HSP 8 Score: 45.0542 bits (105), Expect = 6.253e-7
Identity = 27/111 (24.32%), Postives = 48/111 (43.24%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K   E+  +LLE   +      Q   P+H A  + +++MV LLL R+ +I      G   +H A   +   + E+L     ++
Sbjct:  590 VALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADV 700          

HSP 9 Score: 45.0542 bits (105), Expect = 7.303e-7
Identity = 25/105 (23.81%), Postives = 47/105 (44.76%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +V LL+  + +I   TK  L  +H A  ++   +  +L +   D+       + P+H A +  N +MVN LL     +      G+ P+H A    ++ ++  L 
Sbjct:  656 MVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760          

HSP 10 Score: 44.669 bits (104), Expect = 1.016e-6
Identity = 27/107 (25.23%), Postives = 47/107 (43.93%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +++LL++H  +I   T+  L PIH A    +  +  LLL+        + +   P+H A      E+V  LL     +         P+H A     +EI+++L  R
Sbjct:  392 VMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQR 498          

HSP 11 Score: 44.2838 bits (103), Expect = 1.076e-6
Identity = 26/113 (23.01%), Postives = 52/113 (46.02%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +  LL+E   + + K K  L P+H A   ++ ++  LLL++           + P+H A  K   E+  +LL+          +G  P+H A    +++++ +L  R+ +I
Sbjct:  555 LEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASI 667          
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 1.247e-10
Identity = 25/89 (28.09%), Postives = 48/89 (53.93%), Query Frame = -2
Query:    1 IHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 267
            +H A    +SE+ N L+ +  +I   D +  R IHWA    ++E+V +L+    ++ C D +G+ P+H A  +    ++++L D   +I
Sbjct:  142 LHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDI 230          

HSP 2 Score: 54.6842 bits (130), Expect = 3.108e-10
Identity = 30/111 (27.03%), Postives = 54/111 (48.65%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V  L+    NI    K+  R IH+A    ++E+  +L+    D+ C+D + + P+H A +     +V LLLD   +I+  ++ G   +H A  N    ++  L D   N+
Sbjct:  153 MVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANV 263          

HSP 3 Score: 52.7582 bits (125), Expect = 1.414e-9
Identity = 29/112 (25.89%), Postives = 57/112 (50.89%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEI-LEMLFDRNCNI 333
            +V++L+ H  ++ CK K+   P+H A       +  LLL+   DI+  ++     +H A       +VN L+D   N+   + RG+ P+H+A  + +  + LE+L +   ++
Sbjct:  186 VVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADV 297          

HSP 4 Score: 43.5134 bits (101), Expect = 1.968e-6
Identity = 27/114 (23.68%), Postives = 54/114 (47.37%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLR-PIHFAIMKENSEIFNLLLER---NCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V++LI+H  ++  K     R  +H A    + E   LL+E    N  ++  D     P+  A+   +++  +LLL +  +I   DN+G   +H      + E +++L  R+ ++
Sbjct:  632 VRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDV 745          

HSP 5 Score: 43.5134 bits (101), Expect = 2.065e-6
Identity = 26/107 (24.30%), Postives = 47/107 (43.93%), Query Frame = -2
Query:   16 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKN----SEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 324
            LL+    +I  K  +    +H      + E  +LLL+R+ D+   + +   P+H A    +    S +V   +     I   D  G+ P+HWA  N +   +E+L +
Sbjct:  704 LLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLE 810          

HSP 6 Score: 43.1282 bits (100), Expect = 2.789e-6
Identity = 27/106 (25.47%), Postives = 52/106 (49.06%), Query Frame = -2
Query:   22 VQLLIEH---NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 330
            ++LL+E+   N  ++     +  P+  A+   +++  +LLL +   I  +DN+    +H      + E V+LLL R+ ++   + RG  P+H A    +  IL  L
Sbjct:  666 LRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSAL 771          

HSP 7 Score: 42.743 bits (99), Expect = 4.110e-6
Identity = 25/111 (22.52%), Postives = 46/111 (41.44%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL+     +  K  + L P+H A    +      LL+ + D+   D     P+H A   +  +    ++    +    D  G   +H A  N +SE++  L  +  NI
Sbjct:   54 IIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANI 164          

HSP 8 Score: 41.9726 bits (97), Expect = 8.667e-6
Identity = 27/111 (24.32%), Postives = 49/111 (44.14%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCN--IECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++VQ  +     I    +    P+H+A    +     LLLE +   + ++   + P+H A+   N     +L+D      I C D +G  P+H A   ++ E L++L   N
Sbjct:  770 ALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDV-FQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHN 879          
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007954.1 (pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gene:ENSPMAG00000007165.1 transcript:ENSPMAT00000007954.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.373 bits (124), Expect = 1.569e-9
Identity = 23/95 (24.21%), Postives = 47/95 (49.47%), Query Frame = -2
Query:   19 NIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 303
            N+    +     +H A    N E+ NLL+ +  ++   D +  R +HWA    ++E+V +LL    ++   D +G+ P+H A  + +  ++ +L 
Sbjct:   39 NVNVSDRAGRTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLL 133          

HSP 2 Score: 50.0618 bits (118), Expect = 1.094e-8
Identity = 26/106 (24.53%), Postives = 48/106 (45.28%), Query Frame = -2
Query:    1 IEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 318
            + H  ++  + K    P+H A      +  + L+    ++   D      +H A    N EMVNLL+ +  N+   D R  R +HWA    ++E+++ML     ++
Sbjct:    1 LRHAADVNARDKNWQTPLHVASANRAIKCADALIPLLSNVNVSDRAGRTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADV 106          

HSP 3 Score: 46.595 bits (109), Expect = 2.090e-7
Identity = 27/108 (25.00%), Postives = 49/108 (45.37%), Query Frame = -2
Query:   16 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 339
              +V LLI    N+    K++ R +H+A    ++E+  +LL    D+  +D + + P+H A    +  +V LLL     +   +  G   +H A  N    ++  L D
Sbjct:   60 LEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNTALHVACHNGQDAVVSELLD 167          

HSP 4 Score: 45.0542 bits (105), Expect = 6.501e-7
Identity = 29/107 (27.10%), Postives = 48/107 (44.86%), Query Frame = -2
Query:   16 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCD--IECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 330
            V+LL+E  C  +        P+H A+M +N     +L++      + C+D +   P+H A    N E   LLL     ++  D RG  P+    +   S I+E L +
Sbjct:  670 VELLLEQLC--QKFEGNPFTPLHCAVMNDNESCAEMLIDAMGAKIVMCQDGKGRTPLHAAAFADNVECAQLLLSHGARVDATDRRGRTPLMTGALQGQSGIVEYLVN 774          
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.6026 bits (122), Expect = 3.283e-9
Identity = 28/109 (25.69%), Postives = 52/109 (47.71%), Query Frame = -2
Query:   13 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 339
              IVQLL++H  + +  T     P+H A  + + ++  +LLE    +     + + P+H A      E+ +LLL R  + +     G  P+H A   +N ++  +L D+
Sbjct:  127 LEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 235          

HSP 2 Score: 50.0618 bits (118), Expect = 1.032e-8
Identity = 28/106 (26.42%), Postives = 49/106 (46.23%), Query Frame = -2
Query:   16 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFD 333
            +  +L+EH  ++   TK+   P+H A      E+ +LLL+R    +        P+H A +  N ++  LLLD+  +       G+ P+H A   +  EI   L +
Sbjct:  162 VTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLE 267          

HSP 3 Score: 45.8246 bits (107), Expect = 3.051e-7
Identity = 23/107 (21.50%), Postives = 51/107 (47.66%), Query Frame = -2
Query:   13 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDR 333
            +V+ L+ +   ++ K +++  P+H A      EI  LLL+     +   +  + P+H A  + + ++  +LL+   ++     +G+ P+H A      E+  +L  R
Sbjct:   96 VVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQR 202          

HSP 4 Score: 45.8246 bits (107), Expect = 4.082e-7
Identity = 23/105 (21.90%), Postives = 47/105 (44.76%), Query Frame = -2
Query:   19 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 333
            +  LL+    ++   TK  L P+H A  ++   +  +L++    +       + P+  A +  N +MVN LL    ++      G+ P+H A    ++ I+ +L 
Sbjct:  294 VASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          

HSP 5 Score: 44.669 bits (104), Expect = 7.893e-7
Identity = 25/111 (22.52%), Postives = 47/111 (42.34%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +  LL++   +     K    P+H A  K+  EI   LLE   +      Q   P+H    + ++++ +LLL R  ++      G  P+H A   +   + E+L     ++
Sbjct:  228 VALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHV 338          

HSP 6 Score: 43.5134 bits (101), Expect = 2.652e-6
Identity = 19/73 (26.03%), Postives = 39/73 (53.42%), Query Frame = -2
Query:  118 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLL 336
            ++ ++L++H  ++   TK    P+  A    N ++ N LL+   D+  +    + P+H A  + ++ +VNLLL
Sbjct:  326 NVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          
BLAST of SMED30005565 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560])

HSP 1 Score: 49.2914 bits (116), Expect = 1.500e-8
Identity = 30/107 (28.04%), Postives = 52/107 (48.60%), Query Frame = -2
Query:   31 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLL------ERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEIL 333
            IV LL++H  +   K +Q L  +HF++   N  +   +L        N DI+ +DN    P+ WA  + +   V LLL     +   DNRG+  +H A++  +  ++
Sbjct:  133 IVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVI 239          
BLAST of SMED30005565 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 46.9802 bits (110), Expect = 9.965e-8
Identity = 27/109 (24.77%), Postives = 49/109 (44.95%), Query Frame = -2
Query:   22 IVQLLIEHNCNIECKTKQKLRPIHFAIMKEN-----SEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 333
            IV  L++H  +      Q    +H ++   N       +FN++ +   DI+C D +    + WA  + +S  V  LL    +I+  D  G+ P+HW  +     +L+ L
Sbjct:  158 IVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYL 266          
BLAST of SMED30005565 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 45.4394 bits (106), Expect = 2.355e-7
Identity = 28/92 (30.43%), Postives = 50/92 (54.35%), Query Frame = -2
Query:   13 PIHFAIMKENSEIFNL--LLERNCDIECEDNQRWR-PIHWAINKKNSEMVNLLLDRNCNI---ECGDNRGWRPIHWAIINENSEILEMLFDR 270
            P+H A M+  +E F +  LL     +  + +Q  R P+HW+++ +  E+ + LL +  N+   +  D+ GW P H A    N E+++ L+DR
Sbjct:    5 PLHQACME--NEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDR 94          
BLAST of SMED30005565 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 42.3578 bits (98), Expect = 3.393e-6
Identity = 23/82 (28.05%), Postives = 44/82 (53.66%), Query Frame = -2
Query:   91 IVQLLIEHNCNI-ECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECG 333
            ++QLLI +  +  E     K  PI +A+   +SE+   LL+ N  ++ ED+    P+ +A+ + + +++N LL R  N+   
Sbjct:  573 LIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQRPLNLPSA 654          
BLAST of SMED30005565 vs. Ensembl Nematostella
Match: EDO35626 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SL14])

HSP 1 Score: 50.447 bits (119), Expect = 1.832e-9
Identity = 20/83 (24.10%), Postives = 43/83 (51.81%), Query Frame = -2
Query:   28 LRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILE 276
            +  +H +  +  S   N+L++   D++C D + W P+H A+   N +    LL +  N+   ++ G  PIH A+   + ++++
Sbjct:   16 VTALHESSYEGKSRCVNVLVQLGADVDCRDREGWTPLHAAVCGGNHKCAAYLLRQGANVRAKNDDGLSPIHIAVQQRDRQMVQ 98          

HSP 2 Score: 42.3578 bits (98), Expect = 1.943e-6
Identity = 15/67 (22.39%), Postives = 38/67 (56.72%), Query Frame = -2
Query:  130 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMV 330
            V +L++   +++C+ ++   P+H A+   N +    LL +  ++  +++    PIH A+ +++ +MV
Sbjct:   31 VNVLVQLGADVDCRDREGWTPLHAAVCGGNHKCAAYLLRQGANVRAKNDDGLSPIHIAVQQRDRQMV 97          
BLAST of SMED30005565 vs. Ensembl Nematostella
Match: EDO34863 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN65])

HSP 1 Score: 49.6766 bits (117), Expect = 1.063e-8
Identity = 21/76 (27.63%), Postives = 42/76 (55.26%), Query Frame = -2
Query:   58 QKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWR-PIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWA 282
            + L P+H A+ K +     LL++   +++C+D +  + P+H AI + + +++  LLD + +I   D  G  P+  A
Sbjct:   79 EGLMPLHIAVAKRDKYAIALLVQNGANVDCKDGKSGKTPLHHAIERNDLQIIKTLLDMSADINATDFSGVSPMAMA 154          
BLAST of SMED30005565 vs. Ensembl Nematostella
Match: EDO35987 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJZ9])

HSP 1 Score: 48.1358 bits (113), Expect = 1.595e-8
Identity = 24/90 (26.67%), Postives = 44/90 (48.89%), Query Frame = -2
Query:   19 PIHFAIMKENSEIFNLL------LERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 270
             +H+A+ +  ++I  LL      LE   D++  D      +H+A  + + EM+ LL+ R  +I   D+ G  P+  A I +  E + +L 
Sbjct:    2 ALHYAVREGFADIARLLIKKGSNLEATTDMDAHDMFGRTALHYAAQQGHMEMIKLLVTRGADIHTRDSEGQTPLFQATIFDQKEAVNVLL 91          
BLAST of SMED30005565 vs. Ensembl Nematostella
Match: EDO48849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHH4])

HSP 1 Score: 47.7506 bits (112), Expect = 1.641e-8
Identity = 29/87 (33.33%), Postives = 41/87 (47.13%), Query Frame = -2
Query:   34 KTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIEC-GDNRGWRPIHWAIINENSEI 291
            K K+    +H A++K N+E    LL    D    DN RW P+H A N  +  +  LLLD    I+  G      P+H A+ N   E+
Sbjct:    2 KNKRGETLLHIAVIKGNTEDVRRLLYEGADPNTRDNARWTPLHEACNHGHVIIAELLLDHGALIDTPGGFDHDTPLHDAVTNNQLEV 88          
BLAST of SMED30005565 vs. Ensembl Nematostella
Match: EDO30889 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZG9])

HSP 1 Score: 46.2098 bits (108), Expect = 1.108e-7
Identity = 22/106 (20.75%), Postives = 48/106 (45.28%), Query Frame = -2
Query:   22 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 339
            F    +L+    +++        P+H A      +I  LL+++  D+   + +   P+H +    +SE+  LLLD+  +    D +   P+  AI +   +++E +
Sbjct:    8 FGTADVLVRRGADVKAVDHHGNTPLHCAAAAGRDDIAKLLIDKWSDVNALNKEDQSPLHLSARVGSSEVTQLLLDKGADPRLLDAQARTPMEMAIDSGRIDVIEAI 113          
BLAST of SMED30005565 vs. Ensembl Medaka
Match: ENSORLT00000027359.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 65.855 bits (159), Expect = 1.623e-13
Identity = 30/106 (28.30%), Postives = 56/106 (52.83%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            S    LI H C+++   K    P+H+A    + E+ NLL+ +  ++  +D +  +PIHWA +    E+V LL+    ++ C D  G+ P+H A ++   +++  L 
Sbjct:   48 SCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 153          

HSP 2 Score: 56.6102 bits (135), Expect = 2.905e-10
Identity = 29/110 (26.36%), Postives = 54/110 (49.09%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL++H   +  + K    P+H A     +     L+   C ++  D     P+H+A +  + EMVNLL+ +  N+   D +  +PIHWA      E++++L     ++
Sbjct:   17 VELLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASV 126          
BLAST of SMED30005565 vs. Ensembl Medaka
Match: ENSORLT00000036677.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 65.855 bits (159), Expect = 1.623e-13
Identity = 30/106 (28.30%), Postives = 56/106 (52.83%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            S    LI H C+++   K    P+H+A    + E+ NLL+ +  ++  +D +  +PIHWA +    E+V LL+    ++ C D  G+ P+H A ++   +++  L 
Sbjct:   48 SCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 153          

HSP 2 Score: 56.6102 bits (135), Expect = 2.905e-10
Identity = 29/110 (26.36%), Postives = 54/110 (49.09%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL++H   +  + K    P+H A     +     L+   C ++  D     P+H+A +  + EMVNLL+ +  N+   D +  +PIHWA      E++++L     ++
Sbjct:   17 VELLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASV 126          
BLAST of SMED30005565 vs. Ensembl Medaka
Match: ENSORLT00000036692.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 65.855 bits (159), Expect = 1.630e-13
Identity = 30/106 (28.30%), Postives = 56/106 (52.83%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            S    LI H C+++   K    P+H+A    + E+ NLL+ +  ++  +D +  +PIHWA +    E+V LL+    ++ C D  G+ P+H A ++   +++  L 
Sbjct:  121 SCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 226          

HSP 2 Score: 56.6102 bits (135), Expect = 2.861e-10
Identity = 29/110 (26.36%), Postives = 54/110 (49.09%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL++H   +  + K    P+H A     +     L+   C ++  D     P+H+A +  + EMVNLL+ +  N+   D +  +PIHWA      E++++L     ++
Sbjct:   90 VELLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASV 199          

HSP 3 Score: 49.6766 bits (117), Expect = 7.462e-8
Identity = 27/111 (24.32%), Postives = 50/111 (45.05%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            ++++LI    NI  K    L P+H A    N     LLL+   ++   D     P+H A     +     L+   C+++  D  G  P+H+A  + + E++ +L  +  N+
Sbjct:   56 VMEILISSGANISAKDTGMLTPLHRAAASRNKRAVELLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANV 166          

HSP 4 Score: 45.4394 bits (106), Expect = 2.526e-6
Identity = 26/110 (23.64%), Postives = 46/110 (41.82%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V  L+ HN N+  + +++  P+H A    +  +  +L+    +I  +D     P+H A   +N   V LLL     +   D     P+H A  N  +     L    C++
Sbjct:   24 VTSLLNHNENVNAQDQEQGTPLHAASYLGDVHVMEILISSGANISAKDTGMLTPLHRAAASRNKRAVELLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSL 133          
BLAST of SMED30005565 vs. Ensembl Medaka
Match: ENSORLT00000018386.2 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 65.855 bits (159), Expect = 1.903e-13
Identity = 30/106 (28.30%), Postives = 56/106 (52.83%), Query Frame = -2
Query:   19 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 336
            S    LI H C+++   K    P+H+A    + E+ NLL+ +  ++  +D +  +PIHWA +    E+V LL+    ++ C D  G+ P+H A ++   +++  L 
Sbjct:  117 SCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 222          

HSP 2 Score: 54.6842 bits (130), Expect = 1.434e-9
Identity = 28/109 (25.69%), Postives = 53/109 (48.62%), Query Frame = -2
Query:    1 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 327
            +LL++H   +  + K    P+H A     +     L+   C ++  D     P+H+A +  + EMVNLL+ +  N+   D +  +PIHWA      E++++L     ++
Sbjct:   87 KLLLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASV 195          
BLAST of SMED30005565 vs. Ensembl Medaka
Match: ENSORLT00000039903.1 (pep primary_assembly:ASM223467v1:15:18469388:18484014:-1 gene:ENSORLG00000030042.1 transcript:ENSORLT00000039903.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 6.976e-13
Identity = 34/108 (31.48%), Postives = 54/108 (50.00%), Query Frame = -2
Query:    1 LLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 324
            LL++++ N + ++K    P+H A    N  +  LLL R   ++        P+H A  + NS MV LLLDR   IE     G  P+H    + + +++E+L DR   I
Sbjct:  196 LLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPI 303          

HSP 2 Score: 51.9878 bits (123), Expect = 1.250e-8
Identity = 26/109 (23.85%), Postives = 50/109 (45.87%), Query Frame = -2
Query:   10 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRN 336
            ++  LL+     ++   +  + P+H A  + NS +  LLL+R   IE +      P+H      + ++V +LLDR   I      G  P+H A   ++   +++L   +
Sbjct:  225 NVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHD 333          

HSP 3 Score: 51.2174 bits (121), Expect = 2.196e-8
Identity = 31/111 (27.93%), Postives = 57/111 (51.35%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V LL+E+    + K K +L  +H A  K++++   LLL+ + + + E    + P+H A +  N  +  LLL+R   ++        P+H A    NS ++++L DR   I
Sbjct:  164 VVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARI 270          

HSP 4 Score: 47.3654 bits (111), Expect = 4.812e-7
Identity = 26/111 (23.42%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            +V++L++    I  KTK  L P+H A   ++     LLL+ +  ++   N     +H A +  + ++  L++D+  N       G+ P+H A       ++E+L     +I
Sbjct:  292 VVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKHGASI 402          

HSP 5 Score: 45.8246 bits (107), Expect = 1.587e-6
Identity = 27/111 (24.32%), Postives = 52/111 (46.85%), Query Frame = -2
Query:    4 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCN 336
            ++V+LL++    IE KTK  L P+H      + ++  +LL+R   I  +      P+H A    +   V LLL  +  ++   N     +H A    + ++ +++ D+  N
Sbjct:  258 NMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDKKAN 368          
BLAST of SMED30005565 vs. Planmine SMEST
Match: SMESG000010564.1 (SMESG000010564.1)

HSP 1 Score: 212.231 bits (539), Expect = 4.792e-65
Identity = 104/109 (95.41%), Postives = 106/109 (97.25%), Query Frame = -2
Query:    1 QLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 327
            +LL   NCNIEC+TKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI
Sbjct:  301 ELLFNRNCNIECETKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 409          

HSP 2 Score: 138.272 bits (347), Expect = 5.628e-39
Identity = 67/111 (60.36%), Postives = 85/111 (76.58%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            IV+LL++HNCNIEC+  +  RP H AI   +SEI  LLL+ NC+IEC DN+ WRPIH+A N+ +SE++ LLLD NCNIEC DN GWRPIH+A    +SEIL++L D NCNI
Sbjct:  134 IVKLLLDHNCNIECEDNEGWRPSHCAIDGGHSEILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLLLDHNCNIECEDNEGWRPIHYATNGGHSEILKLLLDHNCNI 244          

HSP 3 Score: 134.42 bits (337), Expect = 1.162e-37
Identity = 65/110 (59.09%), Postives = 83/110 (75.45%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            ++LL++ NCNIEC   + LRPIH+A    +SEI  LLL+ NC+IECEDN+ WRP H AI+  +SE++ LLLD NCNIEC DN GWRPIH+A    +SEIL++L D NCNI
Sbjct:  102 MKLLLDRNCNIECVDNEGLRPIHYATNGGHSEIVKLLLDHNCNIECEDNEGWRPSHCAIDGGHSEILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLLLDHNCNI 211          

HSP 4 Score: 132.88 bits (333), Expect = 3.983e-37
Identity = 66/113 (58.41%), Postives = 87/113 (76.99%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            F I++LL++  CNIEC+  +K+RPIH+AI  +N EI  LLL+RNC IECED +R RPIH+AI+ +N E++  LLDRNCNIEC +N+G RPIH+AI   N EI ++L D NCNI
Sbjct:  429 FEILKLLLDRKCNIECEDIKKMRPIHYAISYQNFEILELLLDRNCIIECEDIKRMRPIHYAISCENLEVLKSLLDRNCNIECEENKGMRPIHYAISCGNLEIFKLLLDLNCNI 541          

HSP 5 Score: 131.724 bits (330), Expect = 9.631e-37
Identity = 66/113 (58.41%), Postives = 86/113 (76.11%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +++LL++ NC+IE + K   RPIH AI KE+ E   LL  RNC+IECE  Q+ RPIH+AI K+NSE+ NLLL+RNC+IEC DN+ WRPIHWAI  +NSE++ +L DRNCNI
Sbjct:  264 IDLLKLLLDRNCDIEYEDKSGWRPIHHAINKEDWETSELLFNRNCNIECETKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNI 376          

HSP 6 Score: 130.568 bits (327), Expect = 2.720e-36
Identity = 62/105 (59.05%), Postives = 85/105 (80.95%), Query Frame = -2
Query:    1 EHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 315
            + NCNI+C+  + +RPIH+AI  +  EIF LLL+RNC+I+CEDN+  RPIH++I+ +N E++ LLL+RNCNIEC DN+G RPIH+AI   N EIL++LFDR+CNI
Sbjct:  800 DRNCNIDCEDNEGMRPIHYAINCQKLEIFKLLLDRNCNIDCEDNRGMRPIHYSISGENFEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLLFDRSCNI 904          

HSP 7 Score: 129.028 bits (323), Expect = 8.465e-36
Identity = 64/111 (57.66%), Postives = 85/111 (76.58%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I+++L + NCNIEC+ +  +RPIHFAI  +N EI  LLL+R C+IECED ++ RPIH+AI+ +N E++ LLLDRNC IEC D +  RPIH+AI  EN E+L+ L DRNCNI
Sbjct:  398 ILEMLFDRNCNIECQNESGIRPIHFAISYKNFEILKLLLDRKCNIECEDIKKMRPIHYAISYQNFEILELLLDRNCIIECEDIKRMRPIHYAISCENLEVLKSLLDRNCNI 508          

HSP 8 Score: 128.642 bits (322), Expect = 1.260e-35
Identity = 62/113 (54.87%), Postives = 84/113 (74.34%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              I +LL++ NCNI+C+  + +RPIH++I  EN EI  LLLERNC+IEC+DN+  RPIH+AI+  N E++ LL DR+CNI+C DN G RPIH+AI  EN EIL++L  + C I
Sbjct:  825 LEIFKLLLDRNCNIDCEDNRGMRPIHYSISGENFEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLLFDRSCNIDCEDNEGMRPIHYAISCENLEILKLLLQQKCGI 937          

HSP 9 Score: 128.642 bits (322), Expect = 1.323e-35
Identity = 61/111 (54.95%), Postives = 85/111 (76.58%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++HNCNIEC   +  RPIH+A  + +SEI  LLL+ NC+IECEDN+ WRPIH+A N  +SE++ LLLD NCNI+C  N G RPI + I ++N ++L++L DRNC+I
Sbjct:  167 ILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLLLDHNCNIECEDNEGWRPIHYATNGGHSEILKLLLDHNCNIDCETNDGKRPIQFVIESKNIDLLKLLLDRNCDI 277          

HSP 10 Score: 128.642 bits (322), Expect = 1.349e-35
Identity = 69/113 (61.06%), Postives = 88/113 (77.88%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            F I++LL+E NCNIECK  + +RPIH+A   E  EI  LLL+RNC+I+CEDN+  RPIH+AIN +  E+  LLLDRNCNI+C DNRG RPIH++I  EN EIL++L +RNCNI
Sbjct:  759 FEILKLLLELNCNIECKDNKGMRPIHYASSCEKLEILKLLLDRNCNIDCEDNEGMRPIHYAINCQKLEIFKLLLDRNCNIDCEDNRGMRPIHYSISGENFEILKLLLERNCNI 871          

HSP 11 Score: 128.257 bits (321), Expect = 1.501e-35
Identity = 63/113 (55.75%), Postives = 85/113 (75.22%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            F I++LL++ NC IEC+  +++RPIH+AI  EN E+   LL+RNC+IECE+N+  RPIH+AI+  N E+  LLLD NCNIEC DN+G RPIH+ I   N EIL++L D +CNI
Sbjct:  462 FEILELLLDRNCIIECEDIKRMRPIHYAISCENLEVLKSLLDRNCNIECEENKGMRPIHYAISCGNLEIFKLLLDLNCNIECEDNKGMRPIHYVITCGNLEILKLLLDHHCNI 574          

HSP 12 Score: 122.865 bits (307), Expect = 1.478e-33
Identity = 61/103 (59.22%), Postives = 78/103 (75.73%), Query Frame = -2
Query:    1 NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 309
            NCNIECK  + +RPIH+AI   N E   LLL+R C+IEC+DN+  RPIH+AI+  N E++ LL DR+CNIEC DN+G RPIH+AI  EN EIL++L + NCNI
Sbjct:  670 NCNIECKDNKGMRPIHYAISCVNLEKLKLLLDRLCNIECKDNKGMRPIHYAISCGNLEILKLLFDRDCNIECKDNKGMRPIHYAISYENFEILKLLLELNCNI 772          

HSP 13 Score: 121.709 bits (304), Expect = 3.439e-33
Identity = 61/111 (54.95%), Postives = 80/111 (72.07%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++HNCNIEC+  +  RPIH+A    +SEI  LLL+ NC+I+CE N   RPI + I  KN +++ LLLDRNC+IE  D  GWRPIH AI  E+ E  E+LF+RNCNI
Sbjct:  200 ILKLLLDHNCNIECEDNEGWRPIHYATNGGHSEILKLLLDHNCNIDCETNDGKRPIQFVIESKNIDLLKLLLDRNCDIEYEDKSGWRPIHHAINKEDWETSELLFNRNCNI 310          

HSP 14 Score: 120.939 bits (302), Expect = 5.641e-33
Identity = 66/127 (51.97%), Postives = 82/127 (64.57%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTK-----------------QKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            V+LL++ NCNIEC+ K                 Q+ RPIH+A     +E   LLL+RNC+IEC DN+  RPIH+A N  +SE+V LLLD NCNIEC DN GWRP H AI   +SEIL++L D NCNI
Sbjct:   52 VRLLLQLNCNIECENKYGFRPIHCVINTECEDNQERRPIHYASSFGVTEAMKLLLDRNCNIECVDNEGLRPIHYATNGGHSEIVKLLLDHNCNIECEDNEGWRPSHCAIDGGHSEILKLLLDHNCNI 178          

HSP 15 Score: 120.939 bits (302), Expect = 6.849e-33
Identity = 64/113 (56.64%), Postives = 84/113 (74.34%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              I++LL + +CNIECK  + +RPIH+AI  EN EI  LLLE NC+IEC+DN+  RPIH+A + +  E++ LLLDRNCNI+C DN G RPIH+AI  +  EI ++L DRNCNI
Sbjct:  726 LEILKLLFDRDCNIECKDNKGMRPIHYAISYENFEILKLLLELNCNIECKDNKGMRPIHYASSCEKLEILKLLLDRNCNIDCEDNEGMRPIHYAINCQKLEIFKLLLDRNCNI 838          

HSP 16 Score: 119.783 bits (299), Expect = 1.756e-32
Identity = 65/113 (57.52%), Postives = 86/113 (76.11%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              I++LL+  NCNI+C+  + ++PIH+AI  EN EI  LLL+ NC+IEC+DN+  RPIH+AI+  N E + LLLDR CNIEC DN+G RPIH+AI   N EIL++LFDR+CNI
Sbjct:  627 LEILKLLLVRNCNIDCEDNEGMKPIHYAISCENLEILKLLLDLNCNIECKDNKGMRPIHYAISCVNLEKLKLLLDRLCNIECKDNKGMRPIHYAISCGNLEILKLLFDRDCNI 739          

HSP 17 Score: 115.161 bits (287), Expect = 7.513e-31
Identity = 62/110 (56.36%), Postives = 84/110 (76.36%), Query Frame = -2
Query:    1 VQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 330
            ++LL++  CNIECK  + +RPIH+AI   N EI  LL +R+C+IEC+DN+  RPIH+AI+ +N E++ LLL+ NCNIEC DN+G RPIH+A   E  EIL++L DRNCNI
Sbjct:  696 LKLLLDRLCNIECKDNKGMRPIHYAISCGNLEILKLLFDRDCNIECKDNKGMRPIHYAISYENFEILKLLLELNCNIECKDNKGMRPIHYASSCEKLEILKLLLDRNCNI 805          

HSP 18 Score: 112.849 bits (281), Expect = 3.620e-30
Identity = 61/113 (53.98%), Postives = 83/113 (73.45%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              +++ L++ NCNIEC+  + +RPIH+AI   N EIF LLL+ NC+IECEDN+  RPIH+ I   N E++ LLLD +CNIEC DN G +PIH++I  EN EIL++L D NC+I
Sbjct:  495 LEVLKSLLDRNCNIECEENKGMRPIHYAISCGNLEIFKLLLDLNCNIECEDNKGMRPIHYVITCGNLEILKLLLDHHCNIECEDNEGMKPIHYSISCENLEILKLLLDLNCSI 607          

HSP 19 Score: 110.153 bits (274), Expect = 4.020e-29
Identity = 60/113 (53.10%), Postives = 84/113 (74.34%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              I +LL++ NCNIEC+  + +RPIH+ I   N EI  LLL+ +C+IECEDN+  +PIH++I+ +N E++ LLLD NC+IEC +N+G RPIH+AI   N EIL++L  RNCNI
Sbjct:  528 LEIFKLLLDLNCNIECEDNKGMRPIHYVITCGNLEILKLLLDHHCNIECEDNEGMKPIHYSISCENLEILKLLLDLNCSIECEENKGMRPIHYAINCRNLEILKLLLVRNCNI 640          

HSP 20 Score: 108.612 bits (270), Expect = 1.393e-28
Identity = 64/113 (56.64%), Postives = 87/113 (76.99%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
              I++LL++H+CNIEC+  + ++PIH++I  EN EI  LLL+ NC IECE+N+  RPIH+AIN +N E++ LLL RNCNI+C DN G +PIH+AI  EN EIL++L D NCNI
Sbjct:  561 LEILKLLLDHHCNIECEDNEGMKPIHYSISCENLEILKLLLDLNCSIECEENKGMRPIHYAINCRNLEILKLLLVRNCNIDCEDNEGMKPIHYAISCENLEILKLLLDLNCNI 673          

HSP 21 Score: 107.842 bits (268), Expect = 2.422e-28
Identity = 60/103 (58.25%), Postives = 78/103 (75.73%), Query Frame = -2
Query:    1 NCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 309
            NC+IEC+  + +RPIH+AI   N EI  LLL RNC+I+CEDN+  +PIH+AI+ +N E++ LLLD NCNIEC DN+G RPIH+AI   N E L++L DR CNI
Sbjct:  604 NCSIECEENKGMRPIHYAINCRNLEILKLLLVRNCNIDCEDNEGMKPIHYAISCENLEILKLLLDLNCNIECKDNKGMRPIHYAISCVNLEKLKLLLDRLCNI 706          

HSP 22 Score: 96.2857 bits (238), Expect = 3.070e-24
Identity = 49/107 (45.79%), Postives = 71/107 (66.36%), Query Frame = -2
Query:   19 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLF 339
            F I++LL+E NCNIECK  + +RPIH+AI   N EI  LL +R+C+I+CEDN+  RPIH+AI+ +N E++ LLL + C I+     G  P+  A  N   E +++L 
Sbjct:  858 FEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLLFDRSCNIDCEDNEGMRPIHYAISCENLEILKLLLQQKCGIDHRRKDGKSPLDLAKENNRVECVQLLI 964          

HSP 23 Score: 90.8929 bits (224), Expect = 1.999e-22
Identity = 50/107 (46.73%), Postives = 64/107 (59.81%), Query Frame = -2
Query:    1 PIHFAIMKENSEIFNLLLERNCDI-----------------ECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 270
            PIH A  + + +   LLL+ NC+I                 ECEDNQ  RPIH+A +   +E + LLLDRNCNIEC DN G RPIH+A    +SEI+++L D NCNI
Sbjct:   39 PIHIACFEGDIDAVRLLLQLNCNIECENKYGFRPIHCVINTECEDNQERRPIHYASSFGVTEAMKLLLDRNCNIECVDNEGLRPIHYATNGGHSEIVKLLLDHNCNI 145          
BLAST of SMED30005565 vs. Planmine SMEST
Match: SMESG000076364.1 (SMESG000076364.1)

HSP 1 Score: 135.576 bits (340), Expect = 9.073e-42
Identity = 63/113 (55.75%), Postives = 87/113 (76.99%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            FSIVQLL+E NCNIEC+ K + RPIH+A    ++++  LL++RN +IECEDN++WRPIH+A    N+++V LL+DRN NIEC DN  WRPIH+A  N N++++ +L DR CNI
Sbjct:   42 FSIVQLLMERNCNIECEDKWQWRPIHYASWNGHADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNI 154          

HSP 2 Score: 101.293 bits (251), Expect = 2.544e-28
Identity = 52/101 (51.49%), Postives = 70/101 (69.31%), Query Frame = -2
Query:    1 NIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 303
            N++   + KL PIH A  K    I  LL+ERNC+IECED  +WRPIH+A    ++++V LL+DRN NIEC DN  WRPIH+A  N N++++ +L DRN NI
Sbjct:   22 NVKSDAEGKL-PIHIAREKGFFSIVQLLMERNCNIECEDKWQWRPIHYASWNGHADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNI 121          

HSP 3 Score: 94.3597 bits (233), Expect = 1.227e-25
Identity = 42/80 (52.50%), Postives = 62/80 (77.50%), Query Frame = -2
Query:   94 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIEC 333
            +V+LLI+ N NIEC+  ++ RPIH+A    N+++  LL++RN +IECEDN++WRPIH+A    N+++V LL+DR CNIEC
Sbjct:   77 VVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNIEC 156          

HSP 4 Score: 53.9138 bits (128), Expect = 4.921e-10
Identity = 23/49 (46.94%), Postives = 37/49 (75.51%), Query Frame = -2
Query:  187 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECED 333
            +V+LLI+ N NIEC+  ++ RPIH+A    N+++  LL++R C+IECE+
Sbjct:  110 VVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNIECEN 158          
BLAST of SMED30005565 vs. Planmine SMEST
Match: SMESG000076364.1 (SMESG000076364.1)

HSP 1 Score: 135.576 bits (340), Expect = 4.951e-41
Identity = 63/113 (55.75%), Postives = 87/113 (76.99%), Query Frame = -2
Query:    1 FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 339
            FSIVQLL+E NCNIEC+ K + RPIH+A    ++++  LL++RN +IECEDN++WRPIH+A    N+++V LL+DRN NIEC DN  WRPIH+A  N N++++ +L DR CNI
Sbjct:   97 FSIVQLLMERNCNIECEDKWQWRPIHYASWNGHADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNI 209          

HSP 2 Score: 101.293 bits (251), Expect = 9.603e-28
Identity = 52/101 (51.49%), Postives = 70/101 (69.31%), Query Frame = -2
Query:    1 NIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 303
            N++   + KL PIH A  K    I  LL+ERNC+IECED  +WRPIH+A    ++++V LL+DRN NIEC DN  WRPIH+A  N N++++ +L DRN NI
Sbjct:   77 NVKSDAEGKL-PIHIAREKGFFSIVQLLMERNCNIECEDKWQWRPIHYASWNGHADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNI 176          

HSP 3 Score: 93.9745 bits (232), Expect = 5.936e-25
Identity = 42/80 (52.50%), Postives = 62/80 (77.50%), Query Frame = -2
Query:   94 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIEC 333
            +V+LLI+ N NIEC+  ++ RPIH+A    N+++  LL++RN +IECEDN++WRPIH+A    N+++V LL+DR CNIEC
Sbjct:  132 VVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNIEC 211          

HSP 4 Score: 53.5286 bits (127), Expect = 1.295e-9
Identity = 23/49 (46.94%), Postives = 37/49 (75.51%), Query Frame = -2
Query:  187 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECED 333
            +V+LLI+ N NIEC+  ++ RPIH+A    N+++  LL++R C+IECE+
Sbjct:  165 VVRLLIDRNGNIECEDNEQWRPIHYASRNGNADVVRLLIDRKCNIECEN 213          
BLAST of SMED30005565 vs. Planmine SMEST
Match: SMESG000043474.1 (SMESG000043474.1)

HSP 1 Score: 142.895 bits (359), Expect = 1.125e-40
Identity = 64/111 (57.66%), Postives = 85/111 (76.58%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K+  RPIH A    +SEI  LLL+RNC++ECED   WRPIH+A    +S+++ LLLDRNCN+EC D RGWRPIHW   N +SEIL++L DRNCN+
Sbjct:  134 ILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNV 244          

HSP 2 Score: 141.354 bits (355), Expect = 3.850e-40
Identity = 64/112 (57.14%), Postives = 88/112 (78.57%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++V+LL++ NCNIEC+ K ++RPIH A    +SEI  LLL+RNC++ECED + WRPIH A +  +SE++ LLLDRNCN+EC D  GWRPIH+A  N +S+IL++L DRNCN+
Sbjct:  100 AVVRLLLDRNCNIECENKWRMRPIHLACSNGHSEILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNV 211          

HSP 3 Score: 128.642 bits (322), Expect = 1.355e-35
Identity = 60/111 (54.05%), Postives = 82/111 (73.87%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K   RPIH+A    +S+I  LLL+RNC++ECED + WRPIHW  +  +SE++ LLLDRNCN+E  D+ G R IH A  N  S+IL++L DRNCNI
Sbjct:  167 ILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNI 277          

HSP 4 Score: 111.694 bits (278), Expect = 1.038e-29
Identity = 56/111 (50.45%), Postives = 77/111 (69.37%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K+  RPIH+     +SEI  LLL+RNC++E ED+   R IH A +   S+++ LLLDRNCNIEC D RG RPIH A  N ++EIL++L    C I
Sbjct:  200 ILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNIECEDKRGERPIHLASSNGHTEILKLLLQCKCRI 310          

HSP 5 Score: 108.227 bits (269), Expect = 1.966e-28
Identity = 52/90 (57.78%), Postives = 65/90 (72.22%), Query Frame = -2
Query:    1 PIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 270
            PIH A       +  LLL+RNC+IECE+  R RPIH A +  +SE++ LLLDRNCN+EC D RGWRPIH A  N +SEIL++L DRNCN+
Sbjct:   89 PIHIACRNGFIAVVRLLLDRNCNIECENKWRMRPIHLACSNGHSEILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNV 178          

HSP 6 Score: 80.1073 bits (196), Expect = 1.197e-18
Identity = 42/104 (40.38%), Postives = 64/104 (61.54%), Query Frame = -2
Query:   22 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 333
            I++LL++ NCN+E +    +R IH A     S+I  LLL+RNC+IECED +  RPIH A +  ++E++ LLL   C I+C +  G  P+  A    N + +E+L
Sbjct:  233 ILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNIECEDKRGERPIHLASSNGHTEILKLLLQCKCRIDCKNKCGKSPLDLAKQFNNVDCVELL 336          
BLAST of SMED30005565 vs. Planmine SMEST
Match: SMESG000043474.1 (SMESG000043474.1)

HSP 1 Score: 142.895 bits (359), Expect = 1.223e-40
Identity = 64/111 (57.66%), Postives = 85/111 (76.58%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K+  RPIH A    +SEI  LLL+RNC++ECED   WRPIH+A    +S+++ LLLDRNCN+EC D RGWRPIHW   N +SEIL++L DRNCN+
Sbjct:  130 ILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNV 240          

HSP 2 Score: 141.354 bits (355), Expect = 4.270e-40
Identity = 64/112 (57.14%), Postives = 88/112 (78.57%), Query Frame = -2
Query:    1 SIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 336
            ++V+LL++ NCNIEC+ K ++RPIH A    +SEI  LLL+RNC++ECED + WRPIH A +  +SE++ LLLDRNCN+EC D  GWRPIH+A  N +S+IL++L DRNCN+
Sbjct:   96 AVVRLLLDRNCNIECENKWRMRPIHLACSNGHSEILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNV 207          

HSP 3 Score: 128.257 bits (321), Expect = 1.492e-35
Identity = 60/111 (54.05%), Postives = 82/111 (73.87%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K   RPIH+A    +S+I  LLL+RNC++ECED + WRPIHW  +  +SE++ LLLDRNCN+E  D+ G R IH A  N  S+IL++L DRNCNI
Sbjct:  163 ILKLLLDRNCNVECEDKLGWRPIHYASRNGHSDILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNI 273          

HSP 4 Score: 111.694 bits (278), Expect = 1.154e-29
Identity = 56/111 (50.45%), Postives = 77/111 (69.37%), Query Frame = -2
Query:    1 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 333
            I++LL++ NCN+EC+ K+  RPIH+     +SEI  LLL+RNC++E ED+   R IH A +   S+++ LLLDRNCNIEC D RG RPIH A  N ++EIL++L    C I
Sbjct:  196 ILKLLLDRNCNVECEDKRGWRPIHWTYSNGHSEILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNIECEDKRGERPIHLASSNGHTEILKLLLQCKCRI 306          

HSP 5 Score: 107.842 bits (268), Expect = 2.187e-28
Identity = 52/90 (57.78%), Postives = 65/90 (72.22%), Query Frame = -2
Query:    1 PIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEMLFDRNCNI 270
            PIH A       +  LLL+RNC+IECE+  R RPIH A +  +SE++ LLLDRNCN+EC D RGWRPIH A  N +SEIL++L DRNCN+
Sbjct:   85 PIHIACRNGFIAVVRLLLDRNCNIECENKWRMRPIHLACSNGHSEILKLLLDRNCNVECEDKRGWRPIHLACSNGHSEILKLLLDRNCNV 174          

HSP 6 Score: 80.1073 bits (196), Expect = 1.306e-18
Identity = 42/104 (40.38%), Postives = 64/104 (61.54%), Query Frame = -2
Query:   22 IVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECEDNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENSEILEML 333
            I++LL++ NCN+E +    +R IH A     S+I  LLL+RNC+IECED +  RPIH A +  ++E++ LLL   C I+C +  G  P+  A    N + +E+L
Sbjct:  229 ILKLLLDRNCNVESEDHSGMRLIHLASSNGRSDILKLLLDRNCNIECEDKRGERPIHLASSNGHTEILKLLLQCKCRIDCKNKCGKSPLDLAKQFNNVDCVELL 332          
The following BLAST results are available for this feature:
BLAST of SMED30005565 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK33.271e-1230.56ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK33.437e-1230.56ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK33.438e-1230.56ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK33.500e-1230.56ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANKK14.102e-1226.61ankyrin repeat and kinase domain containing 1 [Sou... [more]
back to top
BLAST of SMED30005565 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-448.534e-1128.57AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-448.534e-1128.57AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-448.538e-1128.57AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-448.538e-1128.57AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-448.542e-1128.57AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
back to top
BLAST of SMED30005565 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Atac37.453e-1331.13gene:FBgn0052343 transcript:FBtr0299838[more]
Atac37.453e-1331.13gene:FBgn0052343 transcript:FBtr0299831[more]
Atac37.536e-1331.13gene:FBgn0052343 transcript:FBtr0309236[more]
Atac37.536e-1331.13gene:FBgn0052343 transcript:FBtr0332840[more]
Ank22.200e-1229.63gene:FBgn0261788 transcript:FBtr0334062[more]
back to top
BLAST of SMED30005565 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank3a9.467e-1331.48ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank3a9.467e-1331.48ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
BX469921.19.468e-1331.48ankyrin 3a [Source:NCBI gene;Acc:103909035][more]
BX511162.11.997e-1227.93ankyrin 2a, neuronal [Source:NCBI gene;Acc:568926][more]
ank3b4.296e-1229.63ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
back to top
BLAST of SMED30005565 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gorasp13.062e-1433.33golgi reassembly stacking protein 1 [Source:Xenbas... [more]
cpsf12.658e-1328.30cleavage and polyadenylation specific factor 1 [So... [more]
mib16.939e-1227.93mindbomb E3 ubiquitin protein ligase 1 [Source:NCB... [more]
mib17.269e-1227.93mindbomb E3 ubiquitin protein ligase 1 [Source:NCB... [more]
tpm42.113e-1129.63tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097][more]
back to top
BLAST of SMED30005565 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ankk11.509e-1326.36ankyrin repeat and kinase domain containing 1 [Sou... [more]
Ankrd445.951e-1330.09ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG... [more]
Ank36.220e-1330.56ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank36.224e-1330.56ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank36.229e-1330.56ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
back to top
BLAST of SMED30005565 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9J504|V231_FOWPN4.729e-1329.73Putative ankyrin repeat protein FPV231 OS=Fowlpox ... [more]
sp|B0G124|Y9043_DICDI6.749e-1332.14Ankyrin repeat-containing protein DDB_G0279043 OS=... [more]
sp|Q8BZ25|ANKK1_MOUSE1.055e-1226.36Ankyrin repeat and protein kinase domain-containin... [more]
sp|P0DJE3|LATA_LATHE3.354e-1228.04Alpha-latrotoxin-Lhe1a OS=Latrodectus hesperus OX=... [more]
sp|G5E8K5|ANK3_MOUSE4.369e-1230.56Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV... [more]
back to top
BLAST of SMED30005565 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A162PBA58.384e-2234.82Ankyrin repeat protein OS=Colletotrichum incanum O... [more]
A0A2P1P6S31.720e-1941.82ANK_REP_REGION domain-containing protein OS=Candid... [more]
A0A072NUD02.435e-1932.43ANK_REP_REGION domain-containing protein OS=Exophi... [more]
A0A3G5A9691.143e-1842.34ANK_REP_REGION domain-containing protein (Fragment... [more]
A0A428NB562.637e-1836.61ANK_REP_REGION domain-containing protein (Fragment... [more]
back to top
BLAST of SMED30005565 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankk14.753e-1326.42ankyrin repeat and kinase domain containing 1 [Sou... [more]
ank3b9.367e-1330.56ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
mib11.458e-1227.03mindbomb E3 ubiquitin protein ligase 1 [Source:NCB... [more]
mib11.499e-1227.03mindbomb E3 ubiquitin protein ligase 1 [Source:NCB... [more]
ank3a1.508e-1230.56ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
back to top
BLAST of SMED30005565 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000095.14.646e-1330.56pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000000093.12.410e-1129.73pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000003991.11.247e-1028.09pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1... [more]
ENSPMAT00000007954.11.569e-924.21pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gen... [more]
ENSPMAT00000007449.13.283e-925.69pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
back to top
BLAST of SMED30005565 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
AKR21.500e-828.04Ankyrin repeat-containing protein; member of a fam... [more]
AKR19.965e-824.77Palmitoyl transferase involved in protein palmitoy... [more]
NAS62.355e-730.43Evolutionarily conserved 19S regulatory particle a... [more]
PHO813.393e-628.05Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
back to top
BLAST of SMED30005565 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO356261.832e-924.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348631.063e-827.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359871.595e-826.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO488491.641e-833.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308891.108e-720.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30005565 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000027359.11.623e-1328.30serine/threonine-protein phosphatase 6 regulatory ... [more]
ENSORLT00000036677.11.623e-1328.30serine/threonine-protein phosphatase 6 regulatory ... [more]
ENSORLT00000036692.11.630e-1328.30serine/threonine-protein phosphatase 6 regulatory ... [more]
ENSORLT00000018386.21.903e-1328.30serine/threonine-protein phosphatase 6 regulatory ... [more]
ENSORLT00000039903.16.976e-1331.48pep primary_assembly:ASM223467v1:15:18469388:18484... [more]
back to top
BLAST of SMED30005565 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000010564.14.792e-6595.41SMESG000010564.1[more]
SMESG000076364.19.073e-4255.75SMESG000076364.1[more]
SMESG000076364.14.951e-4155.75SMESG000076364.1[more]
SMESG000043474.11.125e-4057.66SMESG000043474.1[more]
SMESG000043474.11.223e-4057.66SMESG000043474.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005565 ID=SMED30005565|Name=SMED30005565|organism=Schmidtea mediterranea sexual|type=transcript|length=340bp
GATATTGCAGTTGCGATCAAATAACATTTCTAATATTTCTGAATTCTCAT
TAATAATTGCCCAATGAATCGGTCTCCACCCTCGATTGTCTCCACATTCA
ATATTGCAGTTACGATCAAGTAATAAATTCACCATTTCCGAATTCTTTTT
ATTAATCGCCCAGTGAATCGGTCTCCACCTTTGATTGTCTTCACATTCGA
TATCGCAGTTGCGCTCAAGCAATAAATTAAATATTTCTGAATTTTCTTTC
ATGATTGCAAAATGAATAGGTCGAAGTTTTTGTTTGGTCTTACATTCGAT
ATTGCAATTGTGCTCTATCAGCAACTGGACAATGCTGAAA
back to top

protein sequence of SMED30005565-orf-1

>SMED30005565-orf-1 ID=SMED30005565-orf-1|Name=SMED30005565-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp
FSIVQLLIEHNCNIECKTKQKLRPIHFAIMKENSEIFNLLLERNCDIECE
DNQRWRPIHWAINKKNSEMVNLLLDRNCNIECGDNRGWRPIHWAIINENS
EILEMLFDRNCNI
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
Vocabulary: INTERPRO
TermDefinition
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding