TIGR00341 family protein

Overview
NameTIGR00341 family protein
Smed IDSMED30005537
Length (bp)1982
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TIGR00341 family protein (SMED30005537) t-SNE clustered cells

Violin plots show distribution of expression levels for TIGR00341 family protein (SMED30005537) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TIGR00341 family protein (SMED30005537) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TIGR00341 family protein (SMED30005537) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005537

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30005537SMESG000062412.1 dd_Smed_v4_9934_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30005537SMESG000062412.1 dd_Smed_v4_9934_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TIGR00341 family protein vs. Ensembl Fly
Match: CG34109 (gene:FBgn0083945 transcript:FBtr0300423)

HSP 1 Score: 162.925 bits (411), Expect = 1.113e-41
Identity = 115/370 (31.08%), Postives = 198/370 (53.51%), Query Frame = 3 
Query:  276 KRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNA-NATYDKSEDSQNA--FQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSIS--VTXXXXXXXXXXXXCHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISIL--TRRMEYDLKSNATICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLK 1364
            ++ + E+L   +I H TW  S+   F  V F +      +  +  L   G G+   SS+ VL  +  + D++T+A N   D SE++++A  +  F+ S+K+++TV Q+   V+  G L+FDYL  +VTA  +A L L  NN   IVA+ML+SPLM P+++I FG I+  K L++ G   L +   +++L    F   +    + + +   +PT  M  RG  + +   +L+A+ S   ++ ALL  +    IG AIS SLLPP+VN G  + L+ I ++   +R+ + LK+ A     +     +  LY +    L  +F+I G  S  ++  N++ I I    V KIK++++ Y    D ++F++ DL+
Sbjct:  157 EKVLEELLVKLEIEHVTWTTSKKGYFHHVVFPLQAGSPCETTLHCLTELGIGTKLNSSVSVLPCSVSY-DAITDAANMRDDYSEEAEDASKWNNFVESIKSKLTVKQVVDGVRAGGSLSFDYLLLIVTADSLAALGLVENNAPNIVAAMLVSPLMGPVMSITFGAIISDKELMRVGFLCLGLGMFISLLFGFIFGLILGTTEMPWGQNSDWPTEEMRGRGNVRALWMGVLWALTSGTGVAVALLQGSAGPLIGVAISASLLPPVVNCGLFWSLACIWLIYPEKRIPH-LKNEAMNATSA-----YPFLYTN---YLPTEFLINGIVSACLTIVNVICIFITAIIVLKIKEVSAPYTASPDLKRFWETDLR 516
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BLAST of TIGR00341 family protein vs. Ensembl Fly
Match: CG7384 (gene:FBgn0032262 transcript:FBtr0080135)

HSP 1 Score: 117.472 bits (293), Expect = 2.871e-27
Identity = 98/382 (25.65%), Postives = 185/382 (48.43%), Query Frame = 3 
Query:  252 DYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFC--------DSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISV-TXXXXXXXXXXXXCHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDLKSNATICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEA 1370
            D   +T +++ + +IL    I +A W  S +   + ++F++   ER + + E L+  G G  + S++ V+   A+          +     ++T   +   Q  + +F+NSV++R+ V QI ++V+    +TFD+   LV+AA +A+  L  N+   + +SML+SPLM PI+A +FG ++  + L + G+ N  I + T  +    F  ++C            T  M+ R     ++  +  AI S    +  +L  N  + +G AIS SLLPP VN G L+ ++ I        Y +  N        +  +    Y D+      +  + G  S+ ++ +N+L + ++G  V K+K++A   ++    R+F+  D+K A
Sbjct:   84 DIPLDTDQEQVVRQILHRFHIPNAVWLPSLEGNAYHISFSMDFDERYENLYEALQEFGIGDREGSTVSVVSCLAHRSYVQREDTEEQEDQPSSTEHINAQKQGIWDRFMNSVRSRLNVAQIVRDVRQDAAITFDFCILLVSAAVLASFGLVENSTIFLASSMLISPLMGPIIAAIFGTVIQDRSLRRLGMLNELIGILTATLVGFLFGLVVCTTDRKYGIGEGLTEEMLSRCDLHSLVVGVFTAIPSGAAAAIGILGGNIGSLVGVAISASLLPPAVNSGLLWAVACI--------YKIFENDDSLYVDVIKSRR---YSDNQAT---ELAVLGGISMCLTLSNVLCVYLMGILVLKVKEIAP--VIGRKNRQFWKHDIKLA 449
[Close]
BLAST of TIGR00341 family protein vs. Ensembl Fly
Match: CG12446 (gene:FBgn0031201 transcript:FBtr0077343)

HSP 1 Score: 96.6709 bits (239), Expect = 2.161e-20
Identity = 98/401 (24.44%), Postives = 186/401 (46.38%), Query Frame = 3 
Query:  240 NMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHV-----LESTAYFCDSMTNANATYDKSEDSQNAFQ----------------KFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXX-XXXXCHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSV-ISILTRRMEYDLKSNATICRYSWLSPKHQMLYFDDH-CKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEA 1370
            N+K D     S ++ +  I +  ++ +  W +          F++   ER ++++  L+  G G  D+   HV     LE+ A  C  +   +  +D   D                       F++SV+ R+ V Q+ + V+    LTFD++  L+ AA ++ + L  N++  + +SML+SPLM PI+A +FG ++  + L   GL+N  + +   + IGFF   M+C + ++       T  ++ R     +   +  A+ S    + A+L  N  + +G AIS SLLPP VN G L+ L+V + +L    E                P   M+    +  +L+++ ++    S+ ++  NI+ + ++G  V +IK++A      +  +KF+  D++ A
Sbjct:   95 NLKGD----ASLEKLLRAIAKELRLENVFWTHDISGRQVQARFDLLQDERYERLLCTLQDWGVG--DRPGTHVSALTCLETRAKPCLQVPRQD--HDNFVDQHQEHGEGQGQGQGQAQGQGWQSFMDSVRCRLNVNQVVRQVRRDATLTFDFVVLLIAAALLSCVGLVENSFLFLSSSMLISPLMGPIIAAIFGSVIGDRDLRWLGLKNELLGIAASVAIGFFFGAMVCGFGHFFAISKGLTEEIVSRCDTHSLAIGVCTALASGAAGAIAVLGGNTGSLVGVAISASLLPPAVNAGLLWALAVGVHVLPDDHE----------------PLASMVKHRSYSSQLSVELLVCAAVSMALTLLNIICVWLMGTVVLRIKEVAPAV---QRHQKFWHHDIRTA 468
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BLAST of TIGR00341 family protein vs. TrEMBL
Match: A0A0X3PJD2 (Uncharacterized protein MJ0678 OS=Schistocephalus solidus OX=70667 GN=Y678 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 6.391e-66
Identity = 163/446 (36.55%), Postives = 249/446 (55.83%), Query Frame = 3 
Query:  258 LQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIV-YPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTR------RMEYDLKSNAT--ICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTE-TTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELS--KAEKTQFIGDLKQLTHGVTEDSHYQ 1559
            L+ETS +  I +I   + I   TW  S +  FF V F        ++I++ L +   G    SSI ++E  A       +       S+     FQKF++S+K+R+TV Q+Y  + N+G   F+YL FL+ AA +A ++L TN+ A + ASMLLSPLM PI+ I+FG+ L +K + K+GLRN +IS+ I I +G  FG+  HYI+  +  V YPTN MM RG  K +++ ++ A  S  ++S A+LSN+ +A IGNAISLSLLPP VN G  F LSV+++L R       M  +   N T   C Y W+  K+ ++Y D+ C+   +F + G  S  ++  NI++I+I GY V  +K L       E+ R FY KDLKE       +  L+A D  +   R  ++  ++++ +N D L+  + E  Q   D + +  G+ +D H Q
Sbjct:   26 LEETSMEDLIHQIFAEENITQDTWNTSRNGNFFVVEFPALDQNFSERILDRLGYFNVGKTPDSSIMIIEPAAVV--HKKDKEPRRGTSDIPVKHFQKFVSSLKSRLTVAQVYHAISNQGNFNFNYLSFLICAAVVADIALVTNSAASVFASMLLSPLMEPIMCIIFGLSLREKRMTKKGLRNTAISLVICICVGLTFGIAAHYISIFQDTVPYPTNEMMGRGYAKSLIASVVVAAASGVSISFAVLSNSLAAMIGNAISLSLLPPAVNCGQFFLLSVLALLDRPYVMKTTMALEASKNTTQMYCDYWWIQ-KYNLVYVDNLCEAPKEFALLGTVSFCLTLLNIVLIIITGYSVNWLKDLVPKSFTNEETRIFYTKDLKEVRDNYKCVHKLEATDLASQAYREYLR-LSKVYPENGDHLALTEEENEQLTKDFQAVMEGIGKDRHLQ 467
[Close]
BLAST of TIGR00341 family protein vs. TrEMBL
Match: G7YV01 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CLF_111498 PE=4 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.223e-64
Identity = 165/484 (34.09%), Postives = 249/484 (51.45%), Query Frame = 3 
Query:  216 KDKTSSNENMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIV-YPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDL--KSNAT---------ICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTE-TTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEI 1628
            KD     ++  +    E S +  + +IL  + I   TW+ S +  FF V F+V G ++++ I++ L F   G    SSI V+E TA+    +            SQ   Q+F+ S+K+R+ V Q+Y  +K RG    +YLC L+ AA +A ++L TN+   + ASMLLSPLM+PI+ ILFG+ L +  ++K G RN +IS+   + +G  FG I H ++  E+I  YPT  M  RGQ    ++ +L A  S  +++ A LS   +A IGNAISLSLLPP VN G    L  +  LT++ E D+   +N+T          CRY W+   ++ +Y  D C    +F +    S  +   NIL+IL+ GY V K+K +A      E  R+FY KDL E       +  L A D    L     KEY +++  N D+LS  E  Q   D   +  GV  D H + +T  S       + + +++ EI
Sbjct:   18 KDANGGQDSDTHLTSMELSMEEAVQQILDEESIATDTWSTSRNGQFFKVEFSVIGEDKIESILDRLAFFNVGKTVDSSIMVIEPTAF----LRKRERPPKTGHVSQVGIQEFLRSLKSRLCVAQVYNEIKQRGRFDMNYLCNLICAAVVADIALVTNSAGVVFASMLLSPLMDPIMCILFGLTLREPHMLKTGARNTAISLLFCVLLGLSFGYIAHSVSNFERITPYPTGEMKMRGQADSFIASMLVASFSGISVAFATLSKRVAALIGNAISLSLLPPAVNSG-QLLLLSLLALTQKPEVDVIWTTNSTDIFHDSVLRQCRYPWIR-NYKFIYVKDECHAAREFALLSACSFLLVTMNILLILVTGYSVNKLKDMAPRSFTDETVRRFYQKDLPEVIGNYDCLHKLNAGD----LATRAYKEYLRVNQINPDQLSAEESKQIASDYCAVMQGVGRDQHLRTITAESGQEASQFLQSFIQRSEI 491
[Close]
BLAST of TIGR00341 family protein vs. TrEMBL
Match: A0A3R7C8P7 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_13364s PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 1.661e-64
Identity = 165/484 (34.09%), Postives = 249/484 (51.45%), Query Frame = 3 
Query:  216 KDKTSSNENMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIV-YPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDL--KSNAT---------ICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTE-TTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEI 1628
            KD     ++  +    E S +  + +IL  + I   TW+ S +  FF V F+V G ++++ I++ L F   G    SSI V+E TA+    +            SQ   Q+F+ S+K+R+ V Q+Y  +K RG    +YLC L+ AA +A ++L TN+   + ASMLLSPLM+PI+ ILFG+ L +  ++K G RN +IS+   + +G  FG I H ++  E+I  YPT  M  RGQ    ++ +L A  S  +++ A LS   +A IGNAISLSLLPP VN G    L  +  LT++ E D+   +N+T          CRY W+   ++ +Y  D C    +F +    S  +   NIL+IL+ GY V K+K +A      E  R+FY KDL E       +  L A D    L     KEY +++  N D+LS  E  Q   D   +  GV  D H + +T  S       + + +++ EI
Sbjct:   18 KDANGGQDSDTHLTSMELSMEEAVQQILDEESIATDTWSTSRNGQFFKVEFSVIGEDKIESILDRLAFFNVGKTVDSSIMVIEPTAF----LRKRERPPKTGHVSQVGIQEFLRSLKSRLCVAQVYNEIKQRGRFDMNYLCNLICAAVVADIALVTNSAGVVFASMLLSPLMDPIMCILFGLTLREPHMLKTGARNTAISLLFCVLLGLSFGYIAHSVSNFERITPYPTGEMKMRGQADSFIASMLVASFSGISVAFATLSKRVAALIGNAISLSLLPPAVNSG-QLLLLSLLALTQKPEVDVIWTTNSTDIFHDSVLRQCRYPWIR-NYKFIYVKDECHAAREFALLSACSFLLVTMNILLILVTGYSVNKLKDMAPRSFTDETVRRFYQKDLPEVIGNYDCLHKLDAGD----LATRAYKEYLRVNQINPDQLSAEESKQIASDYCAVMQGVGRDQHLRTITAESGQEASQFLQSFIQRSEI 491
[Close]
BLAST of TIGR00341 family protein vs. TrEMBL
Match: A0A4S2LU18 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004867 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.004e-63
Identity = 162/468 (34.62%), Postives = 244/468 (52.14%), Query Frame = 3 
Query:  264 ETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIV-YPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDL--KSNAT---------ICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTE-TTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEI 1628
            E S +  + +IL  + I   TW+ S +  FF V F+V G ++++ I++ L F   G    SSI V+E TA+    +            SQ   Q+F+ S+K+R+ V Q+Y  +K RG    +YLC L+ AA +A ++L TN+   + ASMLLSPLM+PI+ ILFG+ L +  ++K G RN +IS+   + +G  FG I H ++  E+I  YPT  M  RGQ    ++ +L A  S  +++ A LS   +A IGNAISLSLLPP VN G    L  +  LT++ E D+   +N+T          CRY W+   ++ +Y  D C    +F +    S  +   NIL+IL+ GY V K+K +A      E  R+FY KD+ E       +  L A D    L     KEY +++  N D+LS  E  Q   D   +  GV  D H + +T  S       + + +++ EI
Sbjct:   34 ELSMEEAVQQILDEESITTDTWSTSRNGQFFKVEFSVIGEDKIESILDRLAFFNVGKTVDSSIMVIEPTAF----LRKRERPPKTGHVSQVGIQEFLRSLKSRLCVAQVYNEIKQRGRFDMNYLCNLICAAVVADIALVTNSAGVVFASMLLSPLMDPIMCILFGLTLREPHMLKTGARNTAISLLFCVLLGLSFGYIAHSVSNFERITPYPTGEMKMRGQADSFIASMLVASFSGISVAFATLSKRVAALIGNAISLSLLPPAVNSG-QLLLLSLLALTQKPEVDVIWTTNSTDIFHDSVIRQCRYPWIR-NYKFIYVKDECHAAREFALLSACSFLLVTMNILLILVTGYSVNKLKDMAPRSFTDETVRRFYQKDVPEVIGNYDCLHKLDAGD----LATRAYKEYLRVNQINPDQLSAEESKQIASDYCAVMQGVGRDQHIRTITTESGQEASQFLQSFIQRSEI 491
[Close]
BLAST of TIGR00341 family protein vs. TrEMBL
Match: A0A4E0RFU3 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_001465 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.226e-63
Identity = 164/462 (35.50%), Postives = 254/462 (54.98%), Query Frame = 3 
Query:  261 QETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIV-YPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGG---FLFFLSVISILTRRMEYDLKSNATI--CRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTE-TTMKTLKAKDPNTVLLRNMIKEYAQIH--NKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQ 1619
            +E S +  ++ +L  + +   TW+ S++  FF V F+V G E+M++I++ L F   G  + SSI V+E TA+    +  A         SQ AFQ F+ S+K+R+ V Q+Y  +  RG    +YLCFL+ AA +A ++L TN+   + ASMLLSPLM+PI+ ILFG+ L +  ++K+G++N +IS+   + +G  FG + H ++  E+I  YPTN M  RGQ    L+ +L A  S  +++ A LS   +A IGNAISLSLLPP VN G    L  L+    +T     D   N TI  C Y W+   +  +Y DD C+ + +F + G FS  +   NIL+IL+ GY V  +K +A         R+FY KD+ E       +  L A +    L     KEY +++  N + DELS  EK +   D + +  GV +D H + ++ N+   + + +   +KQ
Sbjct:   35 EELSMEEAVNLVLHEESVVTDTWSVSKNGKFFKVEFSVVGEEKMERILDRLAFYNVGKTEDSSIMVIEPTAFVYKRVKPAKC----GAVSQLAFQDFLRSLKSRLCVAQVYNEINQRGTFDMNYLCFLICAAVVADVALVTNSAGVVFASMLLSPLMDPIVCILFGLNLREPHMLKKGVKNTAISLMFCVGLGLSFGYVAHSVSNFERITPYPTNEMKARGQVISFLASMLVASFSGISVAFATLSKRVAALIGNAISLSLLPPAVNCGQLLLLSLLASSPTITGVTTSD-NPNMTITRCSYPWVR-DYNYIYVDDPCEASKEFAMLGAFSFLLVTMNILLILVTGYTVNTLKDMAPRAFTDATVRRFYQKDVPEVRANYDCLHKLDAGN----LASCAYKEYLRLNQINPDTDELSFEEKERIASDFRAVLQGVEQDHHLRTVSANAGQADCSFLQTFVKQ 486
[Close]
BLAST of TIGR00341 family protein vs. Planmine SMEST
Match: SMESG000062412.1 (SMESG000062412.1)

HSP 1 Score: 1171.76 bits (3030), Expect = 0.000e+0
Identity = 601/615 (97.72%), Postives = 602/615 (97.89%), Query Frame = 3 
Query:  120 MSFQIRVTALLTENERELLSKNGPFSFYNYNWKDKTSSNENMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTXXXXXXXXXXXXCHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVXXXXXXXXXXXXXXXXQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDLKSNATICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTETTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEISNKLPLDELNNLTVNYLTSRNPQERHDMMRYLAQNYQPADDTSRYKRTSIVNESMINDFEFKLQPKTLYASKKNNSVGRFEIFTTKDVNMSPMPIINEAKDKDIKFHLPGEN 1964
            MSFQIRVTALLTENERELLSKNGPFSFYNYNWKDKTSSNENMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTILIFIGFFFGMICHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVSAPNLSTALLSNNNSAQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDLKSNATICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTETTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEISNKLPLDELNNLTVNYLTSRNPQERHDMMRYLAQNYQPADDTSRYK+             FKLQPKTLYASKKNNSVGRFEIFTTKDVNMSPMPIINEAKDKDIKFHLPGEN
Sbjct:    1 MSFQIRVTALLTENERELLSKNGPFSFYNYNWKDKTSSNENMKYDYLQETSKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRFKGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKARMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLLSPLMNPILAILFGIILHQKGLIKEGLRNLSISVTILIFIGFFFGMICHYINYLEKIVYPTNLMMDRGQNKYILSILLFAIVSAPNLSTALLSNNNSAQIGNAISLSLLPPIVNGGFLFFLSVISILTRRMEYDLKSNATICRYSWLSPKHQMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTYLLKEDARKFYDKDLKEAYTETTMKTLKAKDPNTVLLRNMIKEYAQIHNKNVDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQMEISNKLPLDELNNLTVNYLTSRNPQERHDMMRYLAQNYQPADDTSRYKK-------------FKLQPKTLYASKKNNSVGRFEIFTTKDVNMSPMPIINEAKDKDIKFHLPGEN 602
The following BLAST results are available for this feature:
BLAST of TIGR00341 family protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG341091.113e-4131.08gene:FBgn0083945 transcript:FBtr0300423[more]
CG73842.871e-2725.65gene:FBgn0032262 transcript:FBtr0080135[more]
CG124462.161e-2024.44gene:FBgn0031201 transcript:FBtr0077343[more]
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BLAST of TIGR00341 family protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3PJD26.391e-6636.55Uncharacterized protein MJ0678 OS=Schistocephalus ... [more]
G7YV011.223e-6434.09Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
A0A3R7C8P71.661e-6434.09Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A4S2LU181.004e-6334.62Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4E0RFU31.226e-6335.50Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
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BLAST of TIGR00341 family protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIGR00341 family protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000062412.10.000e+097.72SMESG000062412.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005537 ID=SMED30005537|Name=TIGR00341 family protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1982bp
CTTCAATTGCAAATATAAATCAATAAACTAATCTTTAATCTAATTGGAGG
CATGCAAAACAGTGATTATTATTTTTGCACTAGCAGTATTTGTCAAACTT
AAAGCCAATAATCCAGCAAATGTCCTTTCAAATTAGAGTGACGGCGTTGT
TAACTGAAAATGAAAGAGAACTTCTATCAAAAAATGGACCCTTTTCATTC
TATAATTACAATTGGAAAGACAAAACTTCCAGCAATGAAAATATGAAATA
TGATTATCTACAGGAAACGTCAAAGAAAAGACAAATATCAGAAATTCTCC
AAAATCAGAAAATTCATCATGCTACATGGGCTTACAGTGAAGATCTTATG
TTTTTTACTGTAAACTTCAATGTTTATGGTCATGAAAGAATGGATCAAAT
TATGGAAGATTTACGATTCAAAGGATTCGGATCACATGACCAATCATCAA
TTCATGTATTAGAATCTACAGCCTACTTTTGTGATTCTATGACAAATGCA
AATGCTACATACGATAAATCTGAAGATAGTCAGAATGCCTTTCAAAAATT
TATAAATTCTGTCAAAGCAAGAATGACGGTGGTACAGATTTATCAAAATG
TTAAAAACAGAGGAGAGCTGACGTTTGATTATTTATGCTTCCTGGTGACT
GCTGCTGGTATAGCAACCTTATCATTGTCTACGAACAACTATGCAGGAAT
TGTAGCAAGCATGCTTCTTTCTCCACTTATGAATCCGATTTTAGCGATTC
TTTTTGGAATTATTCTCCATCAAAAAGGTCTTATTAAAGAAGGCTTGCGA
AATTTATCCATTTCAGTAACTATTCTTATTTTTATTGGTTTCTTCTTTGG
AATGATTTGTCATTATATCAATTATTTAGAAAAAATAGTTTATCCAACAA
ATTTAATGATGGATAGAGGCCAAAATAAATACATTTTATCAATTCTCTTA
TTTGCCATAGTTTCTGCTCCGAATTTGAGTACTGCTCTGCTGAGTAATAA
TAATTCTGCACAAATTGGAAATGCCATTTCATTATCTCTGCTTCCTCCTA
TTGTTAATGGAGGATTCTTATTTTTCCTTTCAGTTATTTCTATTCTTACA
CGCAGAATGGAATATGATTTAAAGTCTAATGCAACGATTTGCAGATATTC
GTGGCTGTCCCCAAAGCACCAAATGCTCTACTTTGATGATCATTGTAAAT
TGAATTTAGATTTCGTCATAGGAGGTTGTTTCAGTATTTTCATCTCATTT
GCAAATATTTTGGTCATTCTTATCATAGGTTATTTTGTATTCAAGATAAA
AAAACTGGCATCGACTTATTTGTTAAAAGAAGACGCAAGGAAATTTTATG
ACAAAGATCTAAAGGAAGCTTATACGGAGACAACAATGAAAACTTTAAAG
GCTAAAGATCCCAATACAGTTTTACTTCGTAATATGATTAAAGAATATGC
CCAAATACATAATAAAAATGTGGATGAACTTTCTAAAGCTGAAAAAACCC
AATTTATTGGTGATTTGAAGCAATTGACTCATGGAGTAACAGAAGATTCA
CATTACCAATTATTGACCGGAAACTCTGATAACAGAAATATGAATGATAT
TCCAAATAAACTTAAACAAATGGAAATTAGTAATAAATTACCTCTTGATG
AACTGAACAATCTAACAGTCAATTACCTTACGTCTAGAAACCCTCAAGAA
CGACATGATATGATGAGGTATTTAGCACAAAATTATCAACCAGCCGATGA
TACATCAAGATACAAAAGAACATCAATTGTTAATGAGTCCATGATCAATG
ATTTTGAATTCAAACTACAACCAAAGACACTTTATGCAAGCAAAAAAAAT
AACTCTGTTGGTAGATTTGAAATTTTTACTACAAAAGATGTTAATATGTC
ACCAATGCCAATTATTAACGAAGCTAAAGATAAAGACATTAAGTTTCACT
TACCTGGAGAAAACTAACGCTATTTATCGAAA
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protein sequence of SMED30005537-orf-1

>SMED30005537-orf-1 ID=SMED30005537-orf-1|Name=SMED30005537-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=616bp
MSFQIRVTALLTENERELLSKNGPFSFYNYNWKDKTSSNENMKYDYLQET
SKKRQISEILQNQKIHHATWAYSEDLMFFTVNFNVYGHERMDQIMEDLRF
KGFGSHDQSSIHVLESTAYFCDSMTNANATYDKSEDSQNAFQKFINSVKA
RMTVVQIYQNVKNRGELTFDYLCFLVTAAGIATLSLSTNNYAGIVASMLL
SPLMNPILAILFGIILHQKGLIKEGLRNLSISVTILIFIGFFFGMICHYI
NYLEKIVYPTNLMMDRGQNKYILSILLFAIVSAPNLSTALLSNNNSAQIG
NAISLSLLPPIVNGGFLFFLSVISILTRRMEYDLKSNATICRYSWLSPKH
QMLYFDDHCKLNLDFVIGGCFSIFISFANILVILIIGYFVFKIKKLASTY
LLKEDARKFYDKDLKEAYTETTMKTLKAKDPNTVLLRNMIKEYAQIHNKN
VDELSKAEKTQFIGDLKQLTHGVTEDSHYQLLTGNSDNRNMNDIPNKLKQ
MEISNKLPLDELNNLTVNYLTSRNPQERHDMMRYLAQNYQPADDTSRYKR
TSIVNESMINDFEFKLQPKTLYASKKNNSVGRFEIFTTKDVNMSPMPIIN
EAKDKDIKFHLPGEN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
Vocabulary: INTERPRO
TermDefinition
IPR005052Lectin_leg
IPR005240DUF389
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005240Protein of unknown function DUF389PFAMPF04087DUF389coord: 198..384
e-value: 2.0E-26
score: 92.8
IPR005240Protein of unknown function DUF389PANTHERPTHR20992UNCHARACTERIZEDcoord: 52..518
NoneNo IPR availableTMHMMTMhelixcoord: 365..387
NoneNo IPR availableTMHMMTMhelixcoord: 167..184
NoneNo IPR availableTMHMMTMhelixcoord: 194..216
NoneNo IPR availableTMHMMTMhelixcoord: 305..327
NoneNo IPR availableTMHMMTMhelixcoord: 271..293
NoneNo IPR availableTMHMMTMhelixcoord: 229..251