SMED30005514

Overview
NameSMED30005514
Smed IDSMED30005514
Length (bp)827
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005514 (SMED30005514) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005514 (SMED30005514) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005514 (SMED30005514) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005514 (SMED30005514) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005514

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30005514h1SMcG0010365 SmedASXL_001031SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30005514h1SMcG0010365 dd_Smed_v4_26249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005514h1SMcG0010365 dd_Smed_v4_26249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005514h1SMcG0010365 dd_Smed_v4_26249_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005514 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001978:14510..15385 +4029
Homology
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BLAST of SMED30005514 vs. Planmine SMEST
Match: SMESG000003380.1 (SMESG000003380.1)

HSP 1 Score: 466.463 bits (1199), Expect = 7.761e-169
Identity = 229/231 (99.13%), Postives = 229/231 (99.13%), Query Frame = 3 
Query:   75 MISILEVVIFFGCFSGKLKSAFLWTPKINSNQICNTSEINIGSRDLLDCLAECQLIGCCKVKYFRKTIRCILISGSIPVNNPTGIYDGEFISVSMARSKNKTSISCQNSIKNNTKLKQMGVYFVIETVRTFDEAFQVCKSYHAEVAEFKNTSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDRTTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC 767
            MISILEVVIFFGCFSGKLKSAFLWTPKINSNQICNTSEINIGSRDLLDCLAECQLIGCCKVKYFRKTIRCILISGSIPVNNPTG YDGEFISVSMARSKNKT ISCQNSIKNNTKLKQMGVYFVIETVRTFDEAFQVCKSYHAEVAEFKNTSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDRTTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC
Sbjct:    1 MISILEVVIFFGCFSGKLKSAFLWTPKINSNQICNTSEINIGSRDLLDCLAECQLIGCCKVKYFRKTIRCILISGSIPVNNPTGFYDGEFISVSMARSKNKTFISCQNSIKNNTKLKQMGVYFVIETVRTFDEAFQVCKSYHAEVAEFKNTSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDRTTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC 231
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BLAST of SMED30005514 vs. Planmine SMEST
Match: SMESG000003374.1 (SMESG000003374.1)

HSP 1 Score: 83.1889 bits (204), Expect = 6.401e-19
Identity = 60/206 (29.13%), Postives = 94/206 (45.63%), Query Frame = 3 
Query:  174 CNTSEINIGSRDLLDCLAECQLIGCCKVKYFRKTIRCILISGSIPVNNP---TGIY-----DGEFISVSMARSKNKTSISCQNSIKNNTKLKQMGVYFVIETVRTFDEAFQVCKSYHAEVAEFKNTSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDRTTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC 767
            CN ++ +     LL CL EC    CCKVKY+    RC L   +  + N      IY     +    S +   S   T + C   + NN ++K   +YF+++ V ++ +A + C  Y A +AEFK+ S  K +L    K  +  ++MIGA R     F W+T +               +YL VN   S   +++  + +F VLC C
Sbjct:   16 CNFTKSSAYFDGLLRCLVECTSTKCCKVKYWTSKERCSLFYDNEYLRNSDIRQNIYTLVIGNARNASTTSTLSPKPTKVKCIAEVSNNNQIK-CDLYFMLKNVESYSKANESCGEYFANIAEFKSISNMKKLLIACKKIFLGNLFMIGALRNENGKFIWMTLREELKFSDEPPSGKPGDYLAVNYDASVTSLIDVNSKNFAVLCTC 220
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BLAST of SMED30005514 vs. Planmine SMEST
Match: SMESG000003369.1 (SMESG000003369.1)

HSP 1 Score: 67.781 bits (164), Expect = 2.930e-13
Identity = 53/210 (25.24%), Postives = 97/210 (46.19%), Query Frame = 3 
Query:  171 ICNTSEINIGSRDLLDCLAECQLIGCCKVKYFRKTIRCILISGS----IPVNNPTGIYDGEFISVSMARSKNKTSISCQNSIKNNTKLKQMGV-------YFVIETVRTFDEAFQVCKSYHAEVAEFKNTSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDRTTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC 767
            IC +  +    ++LL CL +C  +GCCK+ + ++T  CIL          V     +Y+  + S +   +    S++ + ++  N  L++M         +F++ T +T       C  Y A VAEF      K VL+       ++++MIGA+R+++  FE I +K  F    +       EYL  N + +    V++ +    +LC+C
Sbjct:   25 ICESRPVKEAFKELLGCLVKCHSLGCCKISFAKETDLCILYYDDNYKGGTVARLGNVYN--YASFATPLTSTGISVTTRGNLGGNC-LEEMQSADFKCEEFFLLRTTKTNQNILNKCDHYRATVAEFPAIFLLKTVLDNCKLVFNNEMFMIGAKRVSDKEFETIKTKKKFPVSISGKKAIYTEYLVTNVEATVFDTVSQYSNICSILCEC 231
The following BLAST results are available for this feature:
BLAST of SMED30005514 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005514 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000003380.17.761e-16999.13SMESG000003380.1[more]
SMESG000003374.16.401e-1929.13SMESG000003374.1[more]
SMESG000003369.12.930e-1325.24SMESG000003369.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005514 ID=SMED30005514|Name=SMED30005514|organism=Schmidtea mediterranea sexual|type=transcript|length=827bp
TCTTCCGATCTCAACTCATTTGATTAGTTGAATTTTATTTACACTAAAAA
TACTGAAAAAATTATTTACTGAAAATGATTTCCATTTTGGAAGTTGTAAT
TTTTTTCGGGTGTTTTTCGGGTAAATTGAAATCGGCATTCTTATGGACAC
CAAAAATTAATTCAAATCAAATTTGTAACACATCTGAAATTAATATCGGA
AGTCGAGACCTACTAGACTGTTTAGCCGAATGCCAACTTATTGGATGCTG
CAAAGTTAAATACTTCCGAAAGACAATACGGTGCATTCTAATATCTGGAA
GCATACCCGTTAATAATCCAACGGGAATTTATGATGGAGAGTTCATATCT
GTTTCAATGGCAAGGTCCAAAAACAAAACATCTATTTCTTGCCAAAACAG
CATAAAAAACAATACGAAATTGAAACAGATGGGAGTCTATTTTGTAATTG
AAACGGTAAGGACTTTCGATGAAGCCTTTCAGGTTTGTAAGTCATATCAT
GCAGAGGTTGCAGAATTTAAAAACACAAGTCAATCGAAAGGTGTCTTGAA
TGGTGTTATTAAACATCAAATTGATAAAGTATATATGATCGGTGCCAGGA
GATTAAATGAGACATCCTTCGAGTGGATAACGTCGAAGATAACTTTTGTT
GCTAAATTTACCGAACTGGATCGAACGACAAATGAGTACCTTGGAGTTAA
CAATAAAAATTCTGGATTATTTATTGTCAATGAAAAAACTATAGATTTTG
GAGTTTTGTGTCAGTGTTAAAAAAAGAAATCAGAAATTCTTGCAAAACTG
GACAACCTTTGTATGATTTTACTGGAG
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protein sequence of SMED30005514-orf-1

>SMED30005514-orf-1 ID=SMED30005514-orf-1|Name=SMED30005514-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=232bp
MISILEVVIFFGCFSGKLKSAFLWTPKINSNQICNTSEINIGSRDLLDCL
AECQLIGCCKVKYFRKTIRCILISGSIPVNNPTGIYDGEFISVSMARSKN
KTSISCQNSIKNNTKLKQMGVYFVIETVRTFDEAFQVCKSYHAEVAEFKN
TSQSKGVLNGVIKHQIDKVYMIGARRLNETSFEWITSKITFVAKFTELDR
TTNEYLGVNNKNSGLFIVNEKTIDFGVLCQC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR016186C-type_lectin-like/link_sf
IPR016187CTDL_fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016186C-type lectin-like/link domain superfamilyGENE3DG3DSA:3.10.100.10coord: 104..231
e-value: 6.2E-7
score: 31.4
NoneNo IPR availableCDDcd00037CLECTcoord: 122..185
e-value: 0.00110683
score: 35.6734
IPR016187C-type lectin foldSUPERFAMILYSSF56436C-type lectin-likecoord: 122..205