SMED30005459

Overview


Name	SMED30005459
Smed ID	SMED30005459
Length (bp)	1942

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30005459 (SMED30005459) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005459 (SMED30005459) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005459 (SMED30005459) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005459 (SMED30005459) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005459

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
protonephridia	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30005459	SMESG000014884.1	Contig46404	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30005459	SMESG000014884.1	Contig46404	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
epidermis	SMED30005459	SMESG000014884.1	dd_Smed_v4_2661_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30005459 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.002637:4777..15120 +	9492

Homology

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BLAST of SMED30005459 vs. Ensembl Human
Match: IFIH1 (interferon induced with helicase C domain 1 [Source:HGNC Symbol;Acc:HGNC:18873])

HSP 1 Score: 51.6026 bits (122), Expect = 4.496e-6
Identity = 35/123 (28.46%), Postives = 57/123 (46.34%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNN----------CSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHD 1362
            F CKNC    C   DI V   +H+V +  E F  + I +    + Q KC +          C    G    + G   P +KI+ F +V +N +++ Q +KKW ++P+  P +  +E   FS +
Sbjct:  905 FLCKNCSVLACSGEDIHVIEKMHHVNMTPE-FKELYI-VRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQ-YKKWVELPITFPNLDYSECCLFSDE 1024

[Close]

BLAST of SMED30005459 vs. Ensembl Human
Match: IFIH1 (interferon induced with helicase C domain 1 [Source:HGNC Symbol;Acc:HGNC:18873])

HSP 1 Score: 51.6026 bits (122), Expect = 4.531e-6
Identity = 35/123 (28.46%), Postives = 57/123 (46.34%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNN----------CSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHD 1362
            F CKNC    C   DI V   +H+V +  E F  + I +    + Q KC +          C    G    + G   P +KI+ F +V +N +++ Q +KKW ++P+  P +  +E   FS +
Sbjct:  866 FLCKNCSVLACSGEDIHVIEKMHHVNMTPE-FKELYI-VRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQ-YKKWVELPITFPNLDYSECCLFSDE 985

[Close]

BLAST of SMED30005459 vs. TrEMBL
Match: G3UQR7 (Interferon induced with helicase C domain 1 OS=Meleagris gallopavo OX=9103 GN=IFIH1 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.316e-7
Identity = 35/114 (30.70%), Postives = 54/114 (47.37%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKI---------EILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSA 1338
            F CKNC + IC   DIRV  N+H+V +  +      I             Q + +I C +C    G+   Y G   P +KI+ F +V E+  +  + FKKW ++P+  P +  A
Sbjct:  879 FLCKNCHKVICSGEDIRVIENMHHVSVKKDFQHLYHIRENRTLQDKHADYQTNVEIICKDCGQPWGNMMVYRGLDLPCLKIRNFVVVFEDKKTTKEIFKKWGELPITFPNLDYA 992

[Close]

BLAST of SMED30005459 vs. TrEMBL
Match: A0A093J523 (Interferon-induced helicase C domain-containing protein 1 (Fragment) OS=Fulmarus glacialis OX=30455 GN=N327_11813 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.340e-7
Identity = 35/115 (30.43%), Postives = 58/115 (50.43%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSM----------KIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSA 1338
            F CKNC + IC   DI+V  N+H+V +  + F S+             +  Q + +I C +C    G+   + G   P +KI+ F +V E+  +  Q FKKW ++P++ P+   A
Sbjct:  796 FLCKNCHKLICSGEDIQVIENMHHVSVKKD-FQSLYHTRENKTLQDKHVDYQTNGEIICKDCGQAWGNMMVHRGLDLPCLKIRNFVVVFEDKKTTKQIFKKWGELPVRFPSFDYA 909

[Close]

BLAST of SMED30005459 vs. TrEMBL
Match: G1NEI2 (Interferon induced with helicase C domain 1 OS=Meleagris gallopavo OX=9103 GN=IFIH1 PE=4 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 2.360e-7
Identity = 35/114 (30.70%), Postives = 56/114 (49.12%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTE---CFSSMKIEILN------QNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSA 1338
            F CKNC + IC   DIRV  N+H+V +  +    +   +   L       Q + +I C +C    G+   Y G   P +KI+ F +V E+  +  + FKKW ++P+  P +  A
Sbjct:  884 FLCKNCHKVICSGEDIRVIENMHHVSVKKDFQHLYHIRENRTLQDKHADYQTNVEIICKDCGQPWGNMMVYRGLDLPCLKIRNFVVVFEDKKTTKEIFKKWGELPITFPNLDYA 997

[Close]

BLAST of SMED30005459 vs. TrEMBL
Match: A0A091JAB7 (Interferon-induced helicase C domain-containing protein 1 (Fragment) OS=Egretta garzetta OX=188379 GN=Z169_02803 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.609e-7
Identity = 36/115 (31.30%), Postives = 58/115 (50.43%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILN----------QNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSA 1338
            F CKNC + IC   DI+V  N+H+V +  + F S+     N          Q + +I C +C    G+   + G   P +KI+ F +V E+  +  Q FKKW ++P++ P+   A
Sbjct:  809 FLCKNCHKLICSGDDIQVIENMHHVSVKKD-FQSLYHTRENKTLQDKHADYQTNGEIICKDCGQAWGNMMVHRGLDLPCLKIRNFVVVFEDKKTTKQIFKKWGELPIRFPSFDYA 922

[Close]

BLAST of SMED30005459 vs. TrEMBL
Match: A0A093BYE3 (Interferon-induced helicase C domain-containing protein 1 OS=Pterocles gutturalis OX=240206 GN=N339_08600 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.943e-7
Identity = 36/115 (31.30%), Postives = 58/115 (50.43%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILN----------QNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSA 1338
            F CKNC + IC   DI+V  N+H+V +  + F S+     N          Q + +I C +C    G+   + G   P +KI+ F +V E+  +  Q FKKW ++P++ P+   A
Sbjct:  878 FLCKNCHKLICSGEDIQVIENMHHVSVKKD-FQSLYHTRENKTLQDKHADYQTNGEIICKDCGQAWGNMMVHRGLDLPCLKIRNFVVVFEDKKTTKQIFKKWGELPVRFPSFDYA 991

[Close]

BLAST of SMED30005459 vs. Ensembl Sea Lamprey
Match: DDX58 (DExD/H-box helicase 58 [Source:HGNC Symbol;Acc:HGNC:19102])

HSP 1 Score: 51.9878 bits (123), Expect = 3.787e-7
Identity = 36/120 (30.00%), Postives = 52/120 (43.33%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQ-------------IKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAEL 1344
             +C  C++ +CKS+DIR+  N HY +I     SS  +E+++    Q             I    C+   G    Y     P IKIQ+F    EN+   +    KWKD P  I   S  E+
Sbjct:  455 LHCIKCKELVCKSSDIRLVENTHYTVI-----SSAFMELVDHKPHQKPMSFDGISKIGKIHHKTCTKDWGITAIYKSLKLPIIKIQSFIFKKENSNVHIV-LNKWKDFPGIIAVFSFEEI 568

[Close]

BLAST of SMED30005459 vs. Ensembl Nematostella
Match: EDO44228 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWA2])

HSP 1 Score: 51.9878 bits (123), Expect = 1.622e-7
Identity = 31/114 (27.19%), Postives = 48/114 (42.11%), Query Frame = 1

Query: 1021 NFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQN----------SCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAIS 1332
             FYC+ C    C++ D R  S  HYV+I+ E F   KIE+                ++ C  C    G      G  +P +KI +F +  E    +L  ++KW +  L I  + 
Sbjct:  123 RFYCRKCNTFACEACDFRCVSGSHYVVISPE-FKRDKIELKPHKKPKLITGITMDTKVHCKKCGQDWGRTAKIHGAEWPLLKINSFVVQIEGGPRKL--YRKWTEAALNIKQVD 233

[Close]

BLAST of SMED30005459 vs. Ensembl Nematostella
Match: EDO47177 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMY2])

HSP 1 Score: 51.9878 bits (123), Expect = 7.186e-7
Identity = 32/119 (26.89%), Postives = 56/119 (47.06%), Query Frame = 1

Query: 1030 CKNCRQNICKSTDI-RVASNIHYVI-------INTECFSSMKIEILN--QNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSL-VCENAASQLQKFKKWKDVPLQIPAISSAELLSF 1353
            C+ C   IC++ +I RV  + H  I       ++TE      +++ +    + ++KC  C+  LG   +Y G   P IKI  F + +  N   +   +K+WK VP ++  +   ELL  
Sbjct:  568 CRKCHVFICQAHEIKRVRDSYHVTIADDFSERVDTEEIPPEHVKVFDGFMFNKKMKCKKCAEPLGAMVNYKGMELPCIKIAQFQVAIMRNGRREFATYKQWKKVPFEVEDMDFCELLEL 686

[Close]

BLAST of SMED30005459 vs. Ensembl Nematostella
Match: EDO44279 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWA7])

HSP 1 Score: 50.8322 bits (120), Expect = 1.287e-6
Identity = 31/114 (27.19%), Postives = 48/114 (42.11%), Query Frame = 1

Query: 1024 FYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQN----------SCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISS 1335
            FYC+ C    C++ D R  S  HYV+I+ E F   KIE+                ++ C  C    G      G  +P +KI +F +  E    +L  ++KW +  L I  +  
Sbjct:  536 FYCRKCNTFACEARDFRCVSGSHYVVISPE-FKRDKIELKPHKKPKLIDGITMDTKVHCKKCGEDWGVTAKIHGAEWPLLKINSFVVQIEGGPRKL--YRKWTEAALNIKQVDD 646

[Close]

BLAST of SMED30005459 vs. Ensembl Medaka
Match: dhx58 (DExH-box helicase 58 [Source:NCBI gene;Acc:101162602])

HSP 1 Score: 36.5798 bits (83), Expect = 6.322e-6
Identity = 13/27 (48.15%), Postives = 19/27 (70.37%), Query Frame = 3

Query:  366 CKNCFQGICKSTDIRLAENIYYIVINP 446
            C+NCFQ +   +DIRL E  +++ INP
Sbjct:  558 CRNCFQPVASGSDIRLLEKAHFVNINP 584

HSP 2 Score: 33.113 bits (74), Expect = 6.322e-6
Identity = 21/59 (35.59%), Postives = 29/59 (49.15%), Query Frame = 1

Query:  478 SCQIKC--SKCSSTLGT*RTYKERL-FPTLKIDSLRLELEGDASSSRLFKKWKDVPFQV 645
             C+I C    C+   G    YK+ +  P + I +  LE  G+  +    KKWKDVPF V
Sbjct:  608 GCRISCNNGNCNKEWGFQMKYKKAIILPNMAICNFALETPGERMT---VKKWKDVPFAV 663

[Close]

BLAST of SMED30005459 vs. Ensembl Medaka
Match: dhx58 (DExH-box helicase 58 [Source:NCBI gene;Acc:101162602])

HSP 1 Score: 36.965 bits (84), Expect = 6.353e-6
Identity = 13/27 (48.15%), Postives = 19/27 (70.37%), Query Frame = 3

Query:  366 CKNCFQGICKSTDIRLAENIYYIVINP 446
            C+NCFQ +   +DIRL E  +++ INP
Sbjct:  608 CRNCFQPVASGSDIRLLEKAHFVNINP 634

HSP 2 Score: 33.113 bits (74), Expect = 6.353e-6
Identity = 21/59 (35.59%), Postives = 29/59 (49.15%), Query Frame = 1

Query:  478 SCQIKC--SKCSSTLGT*RTYKERL-FPTLKIDSLRLELEGDASSSRLFKKWKDVPFQV 645
             C+I C    C+   G    YK+ +  P + I +  LE  G+  +    KKWKDVPF V
Sbjct:  658 GCRISCNNGNCNKEWGFQMKYKKAIILPNMAICNFALETPGERMT---VKKWKDVPFAV 713

[Close]

BLAST of SMED30005459 vs. Planmine SMEST
Match: SMESG000014884.1 (SMESG000014884.1)

HSP 1 Score: 558.14 bits (1437), Expect = 0.000e+0
Identity = 294/324 (90.74%), Postives = 300/324 (92.59%), Query Frame = 1

Query:  415 QKIFIILLSIR---SLMTVFTNPASCQIKCSKCSSTLGT*RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSXXXXXXXXXXXWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 1377
            + I+ I+++ +   SLMTVFTNPASCQIKCSKCSSTLGT                  LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN
Sbjct:  187 ENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLGT------------------LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 492

HSP 2 Score: 194.126 bits (492), Expect = 1.495e-54
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 3

Query:  111 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT-SVKLKEKSNKQAXXXXXXXXXXXXXXNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 446
            CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT SVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP
Sbjct:   84 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTASVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 196

HSP 3 Score: 118.627 bits (296), Expect = 5.483e-28
Identity = 60/118 (50.85%), Postives = 75/118 (63.56%), Query Frame = 1

Query: 1018 WNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSL 1371
            WNF CKNC Q ICKSTDIR+A NI+Y++IN +CFSS+     N  SCQIKC+ CSS LG                  +L  E  AS  + FKKWKDVP Q+PAI+  ELL+F+ ++SL
Sbjct:  166 WNFNCKNCFQGICKSTDIRLAENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLG------------------TLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSL 265

HSP 4 Score: 57.7658 bits (138), Expect = 1.515e-8
Identity = 23/32 (71.88%), Postives = 28/32 (87.50%), Query Frame = 3

Query:  348 PVWNFNCKNCFQGICKSTDIRLAENIYYIVIN 443
            P+WNF CKNC Q ICKSTDIR+A NI+Y++IN
Sbjct:  371 PLWNFYCKNCRQNICKSTDIRVASNIHYVIIN 402

[Close]

BLAST of SMED30005459 vs. Planmine SMEST
Match: SMESG000014884.1 (SMESG000014884.1)

HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0
Identity = 294/324 (90.74%), Postives = 300/324 (92.59%), Query Frame = 1

Query:  415 QKIFIILLSIR---SLMTVFTNPASCQIKCSKCSSTLGT*RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSXXXXXXXXXXXWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 1377
            + I+ I+++ +   SLMTVFTNPASCQIKCSKCSSTLGT                  LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN
Sbjct:  186 ENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLGT------------------LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 491

HSP 2 Score: 198.749 bits (504), Expect = 3.023e-56
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 3

Query:  111 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTSVKLKEKSNKQAXXXXXXXXXXXXXXNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 446
            CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTSVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP
Sbjct:   84 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTSVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 195

HSP 3 Score: 118.242 bits (295), Expect = 5.686e-28
Identity = 60/118 (50.85%), Postives = 75/118 (63.56%), Query Frame = 1

Query: 1018 WNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSL 1371
            WNF CKNC Q ICKSTDIR+A NI+Y++IN +CFSS+     N  SCQIKC+ CSS LG                  +L  E  AS  + FKKWKDVP Q+PAI+  ELL+F+ ++SL
Sbjct:  165 WNFNCKNCFQGICKSTDIRLAENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLG------------------TLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSL 264

HSP 4 Score: 58.151 bits (139), Expect = 1.238e-8
Identity = 27/46 (58.70%), Postives = 34/46 (73.91%), Query Frame = 3

Query:  306 DSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVIN 443
            D   L+  P L+  P+WNF CKNC Q ICKSTDIR+A NI+Y++IN
Sbjct:  357 DQQSLATLPPLTP-PLWNFYCKNCRQNICKSTDIRVASNIHYVIIN 401

[Close]

BLAST of SMED30005459 vs. Planmine SMEST
Match: SMESG000014884.1 (SMESG000014884.1)

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 1

Query:  526 RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSXXXXXXXXXXXWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 1377
            RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN
Sbjct:  199 RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSLCN 482

HSP 2 Score: 196.052 bits (497), Expect = 0.000e+0
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 3

Query:  111 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT-SVKLKEKSNKQAXXXXXXXXXXXXXXNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPL 449
            CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT SVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPL
Sbjct:   84 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTASVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPL 197

HSP 3 Score: 81.6481 bits (200), Expect = 1.338e-26
Identity = 42/83 (50.60%), Postives = 55/83 (66.27%), Query Frame = 1

Query:  448 SLMTVFTNPASCQIKCSKCSSTLGT*RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSL 696
            S+     N  SCQIKC+ CSS LG  ++Y   LFPT+KI +  L  E  AS  + FKKWKDVP Q+PAI+  ELL+F+ ++SL
Sbjct:  398 SMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSL 480

HSP 4 Score: 58.151 bits (139), Expect = 1.338e-26
Identity = 23/32 (71.88%), Postives = 28/32 (87.50%), Query Frame = 3

Query:  348 PVWNFNCKNCFQGICKSTDIRLAENIYYIVIN 443
            P+WNF CKNC Q ICKSTDIR+A NI+Y++IN
Sbjct:  361 PLWNFYCKNCRQNICKSTDIRVASNIHYVIIN 392

HSP 5 Score: 95.9005 bits (237), Expect = 1.392e-20
Identity = 51/118 (43.22%), Postives = 66/118 (55.93%), Query Frame = 1

Query: 1018 WNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSL 1371
            WNF CKNC Q ICKSTDIR+A NI+Y++IN                               ++Y   LFPT+KI +  L  E  AS  + FKKWKDVP Q+PAI+  ELL+F+ ++SL
Sbjct:  166 WNFNCKNCFQGICKSTDIRLAENIYYIVINP----------------------------LPRTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSL 255

[Close]

BLAST of SMED30005459 vs. Planmine SMEST
Match: SMESG000014884.1 (SMESG000014884.1)

HSP 1 Score: 375.941 bits (964), Expect = 7.070e-125
Identity = 205/235 (87.23%), Postives = 211/235 (89.79%), Query Frame = 1

Query:  415 QKIFIILLSIR---SLMTVFTNPASCQIKCSKCSSTLGT*RTYKERLFPTLKIDSLRLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSXXXXXXXXXXXWNFYCKNCRQNICKSTDIRVASNIHYVIINT 1110
            + I+ I+++ +   SLMTVFTNPASCQIKCSKCSSTLGT                  LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINT
Sbjct:  187 ENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLGT------------------LELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSLSYCKSLTAKKLEFKKEYTSNSGNSTVGEYTHEFKAVDITGQTEENVEEKTLENNLEYKITLTKFQETSKDGKSEEIKNCQSGRELQETVPNLDQQSLATLPPLTPPLWNFYCKNCRQNICKSTDIRVASNIHYVIINT 403

HSP 2 Score: 194.897 bits (494), Expect = 1.398e-55
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 3

Query:  111 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT-SVKLKEKSNKQAXXXXXXXXXXXXXXNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 446
            CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT SVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP
Sbjct:   84 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTASVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINP 196

HSP 3 Score: 118.242 bits (295), Expect = 2.105e-28
Identity = 60/118 (50.85%), Postives = 75/118 (63.56%), Query Frame = 1

Query: 1018 WNFYCKNCRQNICKSTDIRVASNIHYVIINTECFSSMKIEILNQNSCQIKCNNCSSLLGHRQSYIGCLFPTIKIQAFSLVCENAASQLQKFKKWKDVPLQIPAISSAELLSFSHDHSL 1371
            WNF CKNC Q ICKSTDIR+A NI+Y++IN +CFSS+     N  SCQIKC+ CSS LG                  +L  E  AS  + FKKWKDVP Q+PAI+  ELL+F+ ++SL
Sbjct:  166 WNFNCKNCFQGICKSTDIRLAENIYYIVINPKCFSSLMTVFTNPASCQIKCSKCSSTLG------------------TLELEGDASSSRLFKKWKDVPFQVPAITEPELLNFTENNSL 265

HSP 4 Score: 59.3066 bits (142), Expect = 4.062e-9
Identity = 24/34 (70.59%), Postives = 29/34 (85.29%), Query Frame = 3

Query:  348 PVWNFNCKNCFQGICKSTDIRLAENIYYIVINPL 449
            P+WNF CKNC Q ICKSTDIR+A NI+Y++IN L
Sbjct:  371 PLWNFYCKNCRQNICKSTDIRVASNIHYVIINTL 404

[Close]

BLAST of SMED30005459 vs. Planmine SMEST
Match: SMESG000014884.1 (SMESG000014884.1)

HSP 1 Score: 206.453 bits (524), Expect = 2.025e-62
Identity = 119/120 (99.17%), Postives = 119/120 (99.17%), Query Frame = 3

Query:  111 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT-SVKLKEKSNKQAXXXXXXXXXXXXXXNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPLLDDGIY 467
            CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNT SVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPLLDDGIY
Sbjct:   84 CGYDESEDFEGQCNQLSNVHITPGLQLLNSKRKETRNTASVKLKEKSNKQAKEIIKKEKEKVKVKNDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPLLDDGIY 203

HSP 2 Score: 55.0694 bits (131), Expect = 2.437e-8
Identity = 22/30 (73.33%), Postives = 26/30 (86.67%), Query Frame = 1

Query: 1018 WNFYCKNCRQNICKSTDIRVASNIHYVIIN 1107
            WNF CKNC Q ICKSTDIR+A NI+Y++IN
Sbjct:  166 WNFNCKNCFQGICKSTDIRLAENIYYIVIN 195

The following BLAST results are available for this feature:

BLAST of SMED30005459 vs. Ensembl Human
BLAST of SMED30005459 vs. Ensembl Celegans
BLAST of SMED30005459 vs. Ensembl Fly
BLAST of SMED30005459 vs. Ensembl Zebrafish
BLAST of SMED30005459 vs. Ensembl Xenopus
BLAST of SMED30005459 vs. Ensembl Mouse
BLAST of SMED30005459 vs. UniProt/SwissProt
BLAST of SMED30005459 vs. TrEMBL
BLAST of SMED30005459 vs. Ensembl Cavefish
BLAST of SMED30005459 vs. Ensembl Sea Lamprey
BLAST of SMED30005459 vs. Ensembl Yeast
BLAST of SMED30005459 vs. Ensembl Nematostella
BLAST of SMED30005459 vs. Ensembl Medaka
BLAST of SMED30005459 vs. Planmine SMEST

BLAST of SMED30005459 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
IFIH1	4.496e-6	28.46	interferon induced with helicase C domain 1 [Sourc...	[more]
IFIH1	4.531e-6	28.46	interferon induced with helicase C domain 1 [Sourc...	[more]

BLAST of SMED30005459 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
G3UQR7	2.316e-7	30.70	Interferon induced with helicase C domain 1 OS=Mel...	[more]
A0A093J523	2.340e-7	30.43	Interferon-induced helicase C domain-containing pr...	[more]
G1NEI2	2.360e-7	30.70	Interferon induced with helicase C domain 1 OS=Mel...	[more]
A0A091JAB7	2.609e-7	31.30	Interferon-induced helicase C domain-containing pr...	[more]
A0A093BYE3	2.943e-7	31.30	Interferon-induced helicase C domain-containing pr...	[more]

BLAST of SMED30005459 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
DDX58	3.787e-7	30.00	DExD/H-box helicase 58 [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of SMED30005459 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005459 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3


Match Name	E-value	Identity	Description
EDO44228	1.622e-7	27.19	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO47177	7.186e-7	26.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO44279	1.287e-6	27.19	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30005459 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dhx58	6.322e-6	48.15	DExH-box helicase 58 [Source:NCBI gene;Acc:1011626...	[more]
dhx58	6.353e-6	48.15	DExH-box helicase 58 [Source:NCBI gene;Acc:1011626...	[more]

BLAST of SMED30005459 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000014884.1	1.495e-54	90.74	SMESG000014884.1	[more]
SMESG000014884.1	3.023e-56	90.74	SMESG000014884.1	[more]
SMESG000014884.1	1.338e-26	100.00	SMESG000014884.1	[more]
SMESG000014884.1	7.070e-125	87.23	SMESG000014884.1	[more]
SMESG000014884.1	2.025e-62	99.17	SMESG000014884.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005459 ID=SMED30005459|Name=SMED30005459|organism=Schmidtea mediterranea sexual|type=transcript|length=1942bp
TATATAACGGCTATGGCTGTGATGACTATGACGACTTTGATAACGGCTAT
GGCTATGATGACTATGACGACTTTGATGATGGCTTTCTTTATGACGACTT
GCACGATGGTTGTGGGTACGACGAATCCGAAGACTTTGAAGGTCAATGCA
ACCAATTAAGCAATGTTCACATAACTCCAGGGCTTCAATTGTTGAATTCC
AAAAGAAAAGAAACACGAAATACATCGGTGAAGTTAAAAGAAAAATCTAA
TAAGCAAGCAAAAGAAATTATTAAAAAAGAAAAAGAAAAGGTAAAGGTAA
AAAATGATAGTTTTCAGTTGTCTCTGGATCCTAATTTATCGGGCCTTCCT
GTGTGGAATTTTAATTGTAAAAATTGTTTTCAAGGTATCTGTAAGTCAAC
GGATATTAGGCTGGCAGAAAATATTTATTATATTGTTATCAATCCGCTCC
TTGATGACGGTATTTACTAATCCAGCTTCCTGTCAGATCAAGTGCAGCAA
ATGCAGCAGTACTTTAGGGACGTAGAGAACCTATAAAGAGCGCTTATTTC
CTACATTAAAAATCGACTCATTGAGATTGGAACTCGAAGGAGACGCATCA
TCATCACGGCTATTCAAAAAATGGAAAGATGTACCTTTTCAAGTTCCAGC
TATTACAGAGCCAGAATTGCTGAATTTTACAGAGAATAACTCACTATCTT
ATTGCAAAAGTCTTACTGCTAAGAAACTAGAATTCAAGAAAGAGTATACT
TCTAACTCTGGAAATTCAACTGTTGGAGAATACACTCATGAGTTCAAAGC
GGTTGACATAACCGGTCAAACGGAAGAGAACGTTGAGGAGAAAACTTTGG
AAAATAATTTGGAATATAAAATAACTTTGACAAAGTTTCAAGAAACAAGT
AAAGACGGAAAATCAGAAGAAATCAAAAACTGTCAAAGTGGAAGGGAGTT
GCAAGAAACTGTCCCAAACCTTGATCAACAGTCCCTTGCAACGTTACCGC
CACTAACGCCACCATTGTGGAATTTCTACTGTAAAAATTGCAGACAAAAC
ATCTGTAAATCCACGGATATTAGAGTGGCTAGCAATATTCATTATGTCAT
CATAAATACCGAATGTTTCAGCTCCATGAAAATTGAAATTCTCAATCAGA
ATTCTTGTCAAATCAAATGCAACAATTGCAGTAGCCTTCTTGGACATCGG
CAAAGTTACATCGGCTGTTTATTCCCGACAATAAAAATTCAGGCTTTCAG
TTTGGTTTGTGAAAATGCCGCTTCTCAACTCCAGAAATTCAAAAAATGGA
AGGATGTACCTTTACAAATACCGGCCATCTCATCGGCAGAATTGTTGAGT
TTCAGTCATGACCACTCGCTGTGCAACTGAAACCAGTGAAGCATAAATGG
ACACAAATTAAAAATTGCACCTTTTGTAATGTTAAATTAAAACAAAATAA
GATTTTGTGATTTAATTTGTTTGATGAAGCGTTAAATTATAAATCTCACA
TAAATCAAGAAGGATAATTTCCTATATTTGTCGTAATTGTTGAAAATGCT
CTACTTTTTATATCTCTTTAATAGAGAAGATTTATGTAAATTGCTCATTT
TTGGTTTTTCCTCTCATTCTCATACAAAGTAACGTTTCTCACTCAGCTGG
CTAAGGAATTAAATGCGAAATGTGAATGTGTACCCTGTTTGTTCAAGATT
TTCATAAAATGGCTACTTCTCTCGCAGAGGTTGGTCGTCCAGCTCATATA
TAACTCGATAATTTTATTCCTCAAAGTAATATAGATATGACACGTGTAAG
CCCCCCGCCATCAGCTCAGAACCCCTCATTGCACCCTGTACGAATTCAAA
CGAAGAAAATCCCACCAACCTCCGATAATCAAAACCGAAAGCTAGTTTTT
CAATTACGTGAATAACTCTAGAATTATCGAGTCAGTATCTCC

protein sequence of SMED30005459-orf-1

>SMED30005459-orf-1 ID=SMED30005459-orf-1|Name=SMED30005459-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
YNGYGCDDYDDFDNGYGYDDYDDFDDGFLYDDLHDGCGYDESEDFEGQCN
QLSNVHITPGLQLLNSKRKETRNTSVKLKEKSNKQAKEIIKKEKEKVKVK
NDSFQLSLDPNLSGLPVWNFNCKNCFQGICKSTDIRLAENIYYIVINPLL
DDGIY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000020	protonephridia
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000101	muscle cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR038557	RLR_C_sf
IPR021673	RLR_CTR

Vocabulary: **molecular function**
Term	Definition
GO:0016817	hydrolase activity, acting on acid anhydrides
GO:0003677	DNA binding
GO:0004386	helicase activity
GO:0005524	ATP binding
GO:0016787	hydrolase activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 61..96
IPR038557	RIG-I-like receptor, C-terminal domain superfamily	GENE3D	G3DSA:2.170.150.30		coord: 116..154 e-value: 1.3E-5 score: 26.9
IPR021673	RIG-I-like receptor, C-terminal regulatory domain	PFAM	PF11648	RIG-I_C-RD	coord: 119..150 e-value: 6.6E-6 score: 26.4
IPR021673	RIG-I-like receptor, C-terminal regulatory domain	PROSITE	PS51789	RLR_CTR	coord: 103..155 score: 11.991

Main menu

SMED30005459

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Owlery

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