SMED30005448

Overview
NameSMED30005448
Smed IDSMED30005448
Length (bp)1749
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005448 (SMED30005448) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005448 (SMED30005448) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005448 (SMED30005448) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005448 (SMED30005448) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005448

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30005448h1SMcG0013700 Contig2808uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005448h1SMcG0013700 Contig2808newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30005448h1SMcG0022512 SmedASXL_011650SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30005448h1SMcG0022512 SmedASXL_011651SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30005448h1SMcG0022512 SmedASXL_077092SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30005448h1SMcG0022512 SmedASXL_008954SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022517 Contig44930newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022517 Contig44930uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022512 Contig43391uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022512 Contig43391newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022512 Contig44930newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005448h1SMcG0022512 Contig44930uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30005448h1SMcG0022512 dd_Smed_v4_21965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005448h1SMcG0022512 dd_Smed_v4_21965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30005448h1SMcG0022512 dd_Smed_v4_21965_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30005448h1SMcG0022512 dd_Smed_v6_22273_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30005448h1SMcG0022512 dd_Smed_v6_21965_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30005448h1SMcG0022517 dd_Smed_v6_2090_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30005448 vs. Planmine SMEST
Match: SMESG000009922.1 (SMESG000009922.1)

HSP 1 Score: 856.67 bits (2212), Expect = 0.000e+0
Identity = 447/485 (92.16%), Postives = 462/485 (95.26%), Query Frame = 1 
Query:  295 AFSVWKDSLKFIKFIDVNLMSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKPGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEIXXXXXXXXXXXXXXXXHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*LFLTKLYSFNATFYIENVEFKKLYLMTSNLTNYXXXXXXXXXXXXXXXXXXLSPLMIIYILSTHGHLYR 1749
            AFSV K SL FI+F+D N+MSC RYADEPM +YRD+ +NLY+ SGWGLY+PDK +PDVP+NNSTM+Y+DW+L+RNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPK GDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE             LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETK+RKRLSPLMIIYILSTHGHLYR
Sbjct:  160 AFSVNKTSLNFIQFVDRNVMSCGRYADEPMATYRDRSNNLYVASGWGLYRPDKKDPDVPDNNSTMLYADWKLYRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKLGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE-------------LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETKERKRLSPLMIIYILSTHGHLYR 631

HSP 2 Score: 53.9138 bits (128), Expect = 2.912e-7
Identity = 39/101 (38.61%), Postives = 51/101 (50.50%), Query Frame = 2 
Query:    5 FSAIMFLEILSFISTDYSV-VKEIDGVVYVGGRNSLSAYQSINEKLLPLVNETYKFPPE-----IFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQ 289
            F+    L ILSF +  +S  +KEID  ++V  +NSL  Y   +     ++N++  F        I NGSDIGF        SE    P SLAFD CLNWK 
Sbjct:    3 FTMNFLLIILSFSANLHSFFIKEIDNEIFVSRKNSLEKYVLSHSSKGIILNKSQVFNWREIEWNIMNGSDIGFRIN----GSEWKKGPYSLAFDNCLNWKH 99
[Close]
BLAST of SMED30005448 vs. Planmine SMEST
Match: SMESG000009922.1 (SMESG000009922.1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 447/485 (92.16%), Postives = 462/485 (95.26%), Query Frame = 1 
Query:  295 AFSVWKDSLKFIKFIDVNLMSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKPGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEIXXXXXXXXXXXXXXXXHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*LFLTKLYSFNATFYIENVEFKKLYLMTSNLTNYXXXXXXXXXXXXXXXXXXLSPLMIIYILSTHGHLYR 1749
            AFSV K SL FI+F+D N+MSC RYADEPM +YRD+ +NLY+ SGWGLY+PDK +PDVP+NNSTM+Y+DW+L+RNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPK GDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE             LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETK+RKRLSPLMIIYILSTHGHLYR
Sbjct:  160 AFSVNKTSLNFIQFVDRNVMSCGRYADEPMATYRDRSNNLYVASGWGLYRPDKKDPDVPDNNSTMLYADWKLYRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKLGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE-------------LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETKERKRLSPLMIIYILSTHGHLYR 631

HSP 2 Score: 53.9138 bits (128), Expect = 2.963e-7
Identity = 39/101 (38.61%), Postives = 51/101 (50.50%), Query Frame = 2 
Query:    5 FSAIMFLEILSFISTDYSV-VKEIDGVVYVGGRNSLSAYQSINEKLLPLVNETYKFPPE-----IFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQ 289
            F+    L ILSF +  +S  +KEID  ++V  +NSL  Y   +     ++N++  F        I NGSDIGF        SE    P SLAFD CLNWK 
Sbjct:    3 FTMNFLLIILSFSANLHSFFIKEIDNEIFVSRKNSLEKYVLSHSSKGIILNKSQVFNWREIEWNIMNGSDIGFRIN----GSEWKKGPYSLAFDNCLNWKH 99
[Close]
BLAST of SMED30005448 vs. Planmine SMEST
Match: SMESG000009922.1 (SMESG000009922.1)

HSP 1 Score: 855.514 bits (2209), Expect = 0.000e+0
Identity = 447/485 (92.16%), Postives = 462/485 (95.26%), Query Frame = 1 
Query:  295 AFSVWKDSLKFIKFIDVNLMSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKPGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEIXXXXXXXXXXXXXXXXHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*LFLTKLYSFNATFYIENVEFKKLYLMTSNLTNYXXXXXXXXXXXXXXXXXXLSPLMIIYILSTHGHLYR 1749
            AFSV K SL FI+F+D N+MSC RYADEPM +YRD+ +NLY+ SGWGLY+PDK +PDVP+NNSTM+Y+DW+L+RNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPK GDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE             LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETK+RKRLSPLMIIYILSTHGHLYR
Sbjct:  156 AFSVNKTSLNFIQFVDRNVMSCGRYADEPMATYRDRSNNLYVASGWGLYRPDKKDPDVPDNNSTMLYADWKLYRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKLGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE-------------LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETKERKRLSPLMIIYILSTHGHLYR 627

HSP 2 Score: 53.1434 bits (126), Expect = 6.136e-7
Identity = 38/96 (39.58%), Postives = 49/96 (51.04%), Query Frame = 2 
Query:   20 FLEILSFISTDYSV-VKEIDGVVYVGGRNSLSAYQSINEKLLPLVNETYKFPPE-----IFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQ 289
             L ILSF +  +S  +KEID  ++V  +NSL  Y   +     ++N++  F        I NGSDIGF        SE    P SLAFD CLNWK 
Sbjct:    4 LLIILSFSANLHSFFIKEIDNEIFVSRKNSLEKYVLSHSSKGIILNKSQVFNWREIEWNIMNGSDIGFRIN----GSEWKKGPYSLAFDNCLNWKH 95
[Close]
BLAST of SMED30005448 vs. Planmine SMEST
Match: SMESG000009922.1 (SMESG000009922.1)

HSP 1 Score: 855.514 bits (2209), Expect = 0.000e+0
Identity = 447/485 (92.16%), Postives = 462/485 (95.26%), Query Frame = 1 
Query:  295 AFSVWKDSLKFIKFIDVNLMSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKPGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEIXXXXXXXXXXXXXXXXHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*LFLTKLYSFNATFYIENVEFKKLYLMTSNLTNYXXXXXXXXXXXXXXXXXXLSPLMIIYILSTHGHLYR 1749
            AFSV K SL FI+F+D N+MSC RYADEPM +YRD+ +NLY+ SGWGLY+PDK +PDVP+NNSTM+Y+DW+L+RNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPK GDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE             LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETK+RKRLSPLMIIYILSTHGHLYR
Sbjct:  156 AFSVNKTSLNFIQFVDRNVMSCGRYADEPMATYRDRSNNLYVASGWGLYRPDKKDPDVPDNNSTMLYADWKLYRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKLGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLLNSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAE-------------LYSFNATFYIENVEFKKLYLMTSNLTNYKMNRMNNETENETKERKRLSPLMIIYILSTHGHLYR 627

HSP 2 Score: 53.1434 bits (126), Expect = 6.082e-7
Identity = 38/96 (39.58%), Postives = 49/96 (51.04%), Query Frame = 2 
Query:   20 FLEILSFISTDYSV-VKEIDGVVYVGGRNSLSAYQSINEKLLPLVNETYKFPPE-----IFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQ 289
             L ILSF +  +S  +KEID  ++V  +NSL  Y   +     ++N++  F        I NGSDIGF        SE    P SLAFD CLNWK 
Sbjct:    4 LLIILSFSANLHSFFIKEIDNEIFVSRKNSLEKYVLSHSSKGIILNKSQVFNWREIEWNIMNGSDIGFRIN----GSEWKKGPYSLAFDNCLNWKH 95
[Close]
BLAST of SMED30005448 vs. Planmine SMEST
Match: SMESG000016292.1 (SMESG000016292.1)

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 314/502 (62.55%), Postives = 369/502 (73.51%), Query Frame = 1 
Query:  295 AFSVWKDSLKFIKFIDVNLMSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNNQEDRQAWQSFMLRICKNDKGFK--------YKISTNGLDGLIGKKYHFDPKPGDTKILHENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQTNVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRPGISGTRASSMGALCQLQINENILDDENSKIA-YHKFNFLEAKNAREEIXXXXXXXXXXXXXXXXHRTNGLTLNPYWNQGVLIGNK-----FSDIPD-VGKCNQD--YKNMTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*LFLTKLYSFNATFYIENVEFKKLYLMTSNLTNYXXXXXXXXXXXXXXXXXXLSPLMIIYILSTHGHLYR 1749
            AFSVWKDSLKFIKFIDVNLMSCARY DEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDV+F++VVKE EKHLYFFGHEINNQYGLNNQEDR AWQSFM+RICKND GFK             +G    IG    FD     T   H+   ++  W+  T HLPT HFT+  K+NFGCYIGKRPR+ISDN+FWGQTNV+Y N L DVI++PN++IK+KT+  N+    C  E H QIPL G+FN PGI GT  SS GALCQLQIN NI ++  SK   YH  N L+A +A EE++LLNS+S SNFYSR+ HRT+GLT+ PYWNQGVLIG+      + ++ D +GKC     +K +TK+ FL ++N+SNT WPNELPNR+SS  SA+CQ+SAAE             LYSFNAT  I  VEF+ ++L+  N+TNY M+ +   T      RK  SPLM + I+STHGHLYR
Sbjct:  152 AFSVWKDSLKFIKFIDVNLMSCARYTDEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSDWRLFRNLFTLPSAMAWVLSSDVHFINVVKETEKHLYFFGHEINNQYGLNNQEDRNAWQSFMVRICKNDVGFKSVPMKEAWLMRHMSGHFYNIGSIGEFDYTLSPT---HDEAGHNPDWFHNTVHLPTKHFTSSQKINFGCYIGKRPRSISDNYFWGQTNVLYLNMLVDVIFDPNKTIKFKTKN-NNKYFGCSKEPHEQIPLIGLFNSPGIRGTAMSSTGALCQLQINSNIFNEIGSKKKPYHLTNILKANDAYEELMLLNSVSVSNFYSRTLHRTSGLTIYPYWNQGVLIGDNVHRKYYKNVFDNIGKCQNSSFFKTLTKDKFLKIMNFSNTGWPNELPNRLSSIPSAVCQFSAAE-------------LYSFNATVLIPGVEFRNVFLVADNITNYYMHELEIATTENPGHRKIFSPLMTVIIISTHGHLYR 636

HSP 2 Score: 188.734 bits (478), Expect = 1.005e-50
Identity = 91/93 (97.85%), Postives = 92/93 (98.92%), Query Frame = 2 
Query:   17 MFLEILSFISTDYSVVKEIDGVVYVGGRNSLSAYQSINEKLLPLVNETYKFPPEIFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQNQ 295
            MFLEILSFISTDYSVVKEIDGVVYVGGRNSLS+YQSINEKLLPLVNETYKFPPEIFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQN 
Sbjct:    1 MFLEILSFISTDYSVVKEIDGVVYVGGRNSLSSYQSINEKLLPLVNETYKFPPEIFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQNH 93
The following BLAST results are available for this feature:
BLAST of SMED30005448 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005448 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009922.12.912e-792.16SMESG000009922.1[more]
SMESG000009922.12.963e-792.16SMESG000009922.1[more]
SMESG000009922.16.136e-792.16SMESG000009922.1[more]
SMESG000009922.16.082e-792.16SMESG000009922.1[more]
SMESG000016292.11.005e-5062.55SMESG000016292.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005448 ID=SMED30005448|Name=SMED30005448|organism=Schmidtea mediterranea sexual|type=transcript|length=1749bp
GATTTTTAGTGCAATAATGTTTCTTGAAATTCTCTCATTCATTTCTACAG
ATTATTCCGTAGTCAAGGAAATTGACGGGGTGGTCTATGTCGGCGGAAGA
AACAGTTTATCGGCCTATCAATCCATAAATGAGAAGCTTCTGCCATTGGT
CAATGAAACGTATAAATTCCCGCCAGAAATATTCAACGGATCAGATATCG
GATTCGAATTTAAAATCCTGGAATTTCCCAGTGAAATCACAAACAATCCG
ACTTCTTTAGCGTTTGACACTTGCCTAAACTGGAAGCAGAATCAGCGTTT
TCCGTGTGGAAGGATTCATTGAAATTCATAAAGTTTATCGATGTAAACCT
GATGAGCTGTGCGCGGTATGCCGATGAACCGATGGTGTCATACAGGGACC
AGTTGGATAACCTATATATCGGTTCCGGTTGGGGACTCTACAAACCGGAT
AAATCAAATCCCGACGTGCCGAACAACAATTCTACAATGATGTATAGTGA
CTGGAGGTTATTCCGAAATTTATTTACTCTTCCTTCAGCAATGGCCTGGG
TGTTGTCAAGTGACGTAAATTTCCTCAGTGTGGTCAAGGAAGACGAAAAA
CATTTGTACTTTTTTGGTCATGAGATTAATAATCAGTACGGACTGAATAA
TCAAGAAGATAGACAAGCTTGGCAAAGTTTTATGTTAAGGATTTGCAAGA
ATGACAAAGGATTCAAATACAAAATTTCAACCAATGGATTGGATGGATTA
ATTGGTAAAAAGTATCATTTTGATCCAAAGCCTGGTGATACTAAAATCCT
ACATGAAAATCTCGACTATAGCGAGGCCTGGTATAAAGGAACGGCTCATT
TACCAACAACGCATTTTACAACTCCATTAAAAGTCAACTTCGGTTGTTAT
ATTGGAAAGAGACCTCGAGCTATCAGTGACAACCATTTCTGGGGGCAGAC
AAATGTCATTTATTTCAACCAATTAGCTGATGTGATTTATAATCCTAATG
AATCTATTAAATACAAAACCGAGAGACCAAATGATGATAATTCGGAATGT
CGGGATGAAAGTCATCGACAAATCCCTTTGACAGGAATATTCAACCGGCC
TGGAATTTCTGGAACAAGAGCCTCTTCTATGGGAGCTCTTTGTCAATTAC
AGATAAATGAGAACATTCTAGATGATGAGAACAGTAAAATTGCTTATCAT
AAATTCAATTTTTTAGAAGCTAAAAATGCACGCGAAGAAATTCTTTTACT
TAATTCAATGAGTTCTTCAAATTTCTATAGTAGATCTTTCCACAGAACTA
ATGGGCTGACATTGAATCCTTACTGGAACCAAGGTGTTCTGATAGGCAAT
AAGTTCAGTGATATTCCAGATGTCGGTAAATGCAATCAAGACTATAAAAA
TATGACAAAAGAAAATTTTTTGATGTTGCTTAACTATTCAAACACAGCCT
GGCCAAATGAGCTCCCCAACCGGATGAGCTCTCCCCTATCAGCCATTTGC
CAATATTCTGCCGCAGAGGTTATTCTTGCATTTTTAGATTAATTATTTCT
CACTAAGCTTTATTCTTTCAATGCGACGTTTTACATAGAAAATGTCGAGT
TCAAGAAACTTTACTTGATGACGTCGAATTTGACGAATTACAAAATGAAT
CGCATGAACAATGAAACGGAGAATGAAACTAAAAAAAGAAAGAGGCTTTC
ACCTCTGATGATCATCTATATCCTATCAACTCATGGCCATTTGTATCGA
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protein sequence of SMED30005448-orf-1

>SMED30005448-orf-1 ID=SMED30005448-orf-1|Name=SMED30005448-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=397bp
MSCARYADEPMVSYRDQLDNLYIGSGWGLYKPDKSNPDVPNNNSTMMYSD
WRLFRNLFTLPSAMAWVLSSDVNFLSVVKEDEKHLYFFGHEINNQYGLNN
QEDRQAWQSFMLRICKNDKGFKYKISTNGLDGLIGKKYHFDPKPGDTKIL
HENLDYSEAWYKGTAHLPTTHFTTPLKVNFGCYIGKRPRAISDNHFWGQT
NVIYFNQLADVIYNPNESIKYKTERPNDDNSECRDESHRQIPLTGIFNRP
GISGTRASSMGALCQLQINENILDDENSKIAYHKFNFLEAKNAREEILLL
NSMSSSNFYSRSFHRTNGLTLNPYWNQGVLIGNKFSDIPDVGKCNQDYKN
MTKENFLMLLNYSNTAWPNELPNRMSSPLSAICQYSAAEVILAFLD*
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protein sequence of SMED30005448-orf-2

>SMED30005448-orf-2 ID=SMED30005448-orf-2|Name=SMED30005448-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
IFSAIMFLEILSFISTDYSVVKEIDGVVYVGGRNSLSAYQSINEKLLPLV
NETYKFPPEIFNGSDIGFEFKILEFPSEITNNPTSLAFDTCLNWKQNQRF
PCGRIH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR001627Semap_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding