Transient receptor potential cation channel subfamily A member 1

Overview
NameTransient receptor potential cation channel subfamily A member 1
Smed IDSMED30005445
Length (bp)2619
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transient receptor potential cation channel subfamily A member 1 (SMED30005445) t-SNE clustered cells

Violin plots show distribution of expression levels for Transient receptor potential cation channel subfamily A member 1 (SMED30005445) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transient receptor potential cation channel subfamily A member 1 (SMED30005445) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transient receptor potential cation channel subfamily A member 1 (SMED30005445) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005445

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30005445h1SMcG0016652 SmedASXL_019544SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30005445h1SMcG0016652 SmedASXL_016876SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30005445h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005445h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005445h1SMcG0016652 dd_Smed_v4_37397_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30005445h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005445h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005445h1SMcG0016652 dd_Smed_v4_18706_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30005445h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005445h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005445h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005445h1SMcG0016652 dd_Smed_v4_12031_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: TRPA1 (transient receptor potential cation channel subfamily A member 1 [Source:HGNC Symbol;Acc:HGNC:497])

HSP 1 Score: 201.445 bits (511), Expect = 4.242e-53
Identity = 201/824 (24.39%), Postives = 380/824 (46.12%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----DPAS--ISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD----LVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSFIFTKSE 2462
            +E+G + +  ++  +N     L  AVQN +L++IK+CLD    I+  +    T  H A+      + KLM      S DI +N  D    + LH A + +  ++   L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D   +   H L V++   +     E +   Q  EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L +A+ N ++ VV   +    W   +  + H++  +   I  +I+   +    LLD C++ +T +K   +Y ++Y F  +    +     P    I E   AL +M +  R++LL HP+  + L +KW  +G     ++   + + +I +T  V++ +  +  +S  +     IN +S  +    + ++  + + ++++ +  I         I Q+ RN F   +N          II   P   ++P   +W+     ++  +  F+ YLQ   + GI ++M + +LKT++R    F  +   F   FYI+   QDPF +  L+ +++ +MM G+ +     +  ++  +  + V S   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P    R +        P + +   ++  +F   F+F   E
Sbjct:   74 EEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDI-VNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASW-----NIVNLLLSKGAQVDIKDNFGRNFLH-LTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGI-----INETSDHSEILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIF--CFLFCTGE 880          

HSP 2 Score: 74.3294 bits (181), Expect = 8.229e-13
Identity = 70/267 (26.22%), Postives = 115/267 (43.07%), Query Frame = 3
Query:  270 LMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECF---RDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L++ R I++N+   + N+ +  A   N  + +++LLK GA     NK    PI  A    S                                   KE +E+I    E    S+++  IN  +    +PLH AV  G+LE+ ++ L+ GA  +      C  +H AA +G  +IVKL+++S   S       D    + +H A+   ++E+  +LI +G D    D  GR+ L+ A  S    I+  LL+K   V++ D      LH
Sbjct:    2 LLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGS-----------------------------------KECMEIILRFGEEHGYSRQLH-INFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRNFLH 232          

HSP 3 Score: 68.5514 bits (166), Expect = 4.668e-11
Identity = 82/387 (21.19%), Postives = 168/387 (43.41%), Query Frame = 3
Query:  189 VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA--------NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEI------FKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLW---TSGAILSD-NMFGQSPLDIAISNNQENVVK-------FFLNDDNWQNVMAYRHDNGSSHIENLI 1274
            +D+N +  +G T    A   N     +++  +       +     P+H A  +   + ++++L+FG         +IN  N    TP+ LA ++   L+    +IK+ +   A ++  +    T  H+   +   E++K +  + S S  ++++N+ D   ++ LH A    + E+   L+  GA+ N   +    PL +A      +IV LLL+   + +  +D+   + +H+  +   Y +      F  +  I +  + +D  G   L +A R G    +  LL    +++   + K + LH AA+ G +   Q L    +   +L++ ++ G +PL +A  N  + VV+        FL+D N    + +    G +    +I
Sbjct:    8 IDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLA-VQNGDLE----MIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGS--VDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVD-IKDNFGRNFLHLTVQQ-PYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVI 385          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: TRPA1 (transient receptor potential cation channel subfamily A member 1 [Source:HGNC Symbol;Acc:HGNC:497])

HSP 1 Score: 200.29 bits (508), Expect = 2.510e-52
Identity = 202/824 (24.51%), Postives = 379/824 (46.00%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----DPAS--ISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSFIFTKSE 2462
            +E+G + +  ++  +N     L  AVQN +L++IK+CLD    I+  +    T  H A+      + KLM      S DI +N  D    + LH A + +  ++   L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D   +   H L V++   +     E +   Q  EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L +A+ N ++ VV   +    W   +  + H++  +   I  +I+   +    LLD C++ +T +K   +Y ++Y F  +    +     P    I E   AL +M +  R++LL HP+  + L +KW  +G     ++   + + +I +T  V++ +  +  +S  +     IN +S  +    + ++  + + ++++ +  I         I Q+ RN F   +N          II   P   ++P   +W+     ++  +  F+ YLQ   + GI ++M + +LKT++R    F  +   F   FYI+   QDPF +  L+ +++ +MM G+ +    F+      +  + V S   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P    R +        P + +   ++  +F   F+F   E
Sbjct:  222 EEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDI-VNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASW-----NIVNLLLSKGAQVDIKDNFGRNFLH-LTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGI-----INETSDHSEILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIF--CFLFCTGE 1028          

HSP 2 Score: 88.9669 bits (219), Expect = 2.213e-17
Identity = 90/336 (26.79%), Postives = 157/336 (46.73%), Query Frame = 3
Query:   81 NVKNALDEKDNDGQCCLSYAVQNKNLDII-KICLDFCVDINTKQAD-GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVV---KEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECF---RDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            N +  L   D+     L YA     ++++ KI  D  +++  +  D G T  H A   N     K + SR  N N+ + ++ +PLH A+     +++KVLL+    ++NL  ++  T +++AC       N S  +++L+ + A     +       H       KE +E+I    E    S+++  IN  +    +PLH AV  G+LE+ ++ L+ GA  +      C  +H AA +G  +IVKL+++S   S       D    + +H A+   ++E+  +LI +G D    D  GR+ L+ A  S    I+  LL+K   V++ D      LH
Sbjct:   51 NKQKKLKRCDDMDTFFLHYAAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACT-----TNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLH-INFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRNFLH 380          

HSP 3 Score: 85.8853 bits (211), Expect = 2.276e-16
Identity = 65/237 (27.43%), Postives = 114/237 (48.10%), Query Frame = 3
Query:  525 DMKTIF-HYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSG---CLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLN 1205
            DM T F HY   + +IE++    E I+    +E+++  D    +PLH AV    +E  + LL  GANPN+   N   PLHIA +  + +++K+LL            N ++A+ IA    N E  + L+  G      +++G   +  AA SG   C+ II     E+  +++ ++N  +  K T LH A   G L++++    +GA +     G+ + +  A +     +VK  ++
Sbjct:   61 DMDTFFLHYAAAEGQIELM----EKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMIS 293          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: RIPK4 (receptor interacting serine/threonine kinase 4 [Source:HGNC Symbol;Acc:HGNC:496])

HSP 1 Score: 128.642 bits (322), Expect = 8.907e-30
Identity = 89/336 (26.49%), Postives = 161/336 (47.92%), Query Frame = 3
Query:  111 NDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            + G   L  AV+    +  K  L    + N     G T  H A       + +L+ +R I++N  D D  + LH A  N      ++LL+  A++N  + + +TP+ +AC           I+++L+    D++++  D     HY   +  + ++K + +    S     +N+Q +  ++PLH A   G+  + ++L++  ++ N+ +     PLH+AAE GH    +LLL+     E    S+  +A+H+AA NG+    K L+    D LA+    +  L  AA  G   ++E L++ +  ++L D+  ++ALH AA   H Q V+ L   GA
Sbjct:  436 DSGASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQH-----GQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVS-----VNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAM-TSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADV-IDLFDEQGLSALHLAAQGRHAQTVETLLRHGA 759          

HSP 2 Score: 106.686 bits (265), Expect = 6.414e-23
Identity = 95/377 (25.20%), Postives = 165/377 (43.77%), Query Frame = 3
Query:  261 LFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-----------------------------------GIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNE 1286
            L K+++ +D+++ +      S LH A+   + +  K LL   AN NL N+   TP+ +A       + + G+++LL+  K  +N +D D  T  H+          + + E  +       +N  D   ++P+H A   G   I ++LL  G + ++   +  LPLH AA +GHL IVKLL      S   +  +  + +H+AA+ G+Y + + LI                                   G GK+ +  D  G   L  AAR+G L  ++ L+ ++ +V     +  TALH AAA GH +VV+ L ++  I   +  G S L +A        V+  L      N+ + +   G      L++ ++
Sbjct:  422 LMKILQPQDVDLALDSG--ASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVE-----RRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNAS------VNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSD--GYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADVIDLFDEQGLSALHLAAQGRHAQTVETLLRHGAHINLQSLKFQGGHGPAATLLRRSK 783          

HSP 3 Score: 91.2781 bits (225), Expect = 3.734e-18
Identity = 82/331 (24.77%), Postives = 140/331 (42.30%), Query Frame = 3
Query:   87 KNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLK-FGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECF-RDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA 1073
            K +++ KD D    L +A QN +    ++ L+    +N    +G+T  H A       + +++  R +++++   D   PLH+A     L +VK+L K  G ++N    D +TP+ LA                                   HY V +  I++                +N   ++ ++PLH A   G+    ++LL  GA      ++    LH+AA  GHL  VKLL+    K++   R     +A+H+AA +G+ E+ + L+      L  DE G + L  AA+      +E LL    ++NL   +K    H  AA
Sbjct:  494 KISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQR-------------------------------GHYRVARILIDLCS-------------DVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSDGYTALHLAARNGHLATVKLLVEE--KADVLARGPLNQTALHLAAAHGHSEVVEELVSADVIDLF-DEQGLSALHLAAQGRHAQTVETLLRHGAHINL-QSLKFQGGHGPAA 776          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: RIPK4 (receptor interacting serine/threonine kinase 4 [Source:HGNC Symbol;Acc:HGNC:496])

HSP 1 Score: 128.642 bits (322), Expect = 9.119e-30
Identity = 95/356 (26.69%), Postives = 170/356 (47.75%), Query Frame = 3
Query:   51 SSKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            +SKL+K   I     +D   + G   L  AV+    +  K  L    + N     G T  H A       + +L+ +R I++N  D D  + LH A  N      ++LL+  A++N  + + +TP+ +AC           I+++L+    D++++  D     HY   +  + ++K + +    S     +N+Q +  ++PLH A   G+  + ++L++  ++ N+ +     PLH+AAE GH    +LLL+     E    S+  +A+H+AA NG+    K L+    D LA+    +  L  AA  G   ++E L++ +  ++L D+  ++ALH AA   H Q V+ L   GA
Sbjct:  467 TSKLMK---ILQPQDVDLALDSGASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQH-----GQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVS-----VNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAM-TSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADV-IDLFDEQGLSALHLAAQGRHAQTVETLLRHGA 807          

HSP 2 Score: 106.686 bits (265), Expect = 6.395e-23
Identity = 95/377 (25.20%), Postives = 165/377 (43.77%), Query Frame = 3
Query:  261 LFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-----------------------------------GIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNE 1286
            L K+++ +D+++ +      S LH A+   + +  K LL   AN NL N+   TP+ +A       + + G+++LL+  K  +N +D D  T  H+          + + E  +       +N  D   ++P+H A   G   I ++LL  G + ++   +  LPLH AA +GHL IVKLL      S   +  +  + +H+AA+ G+Y + + LI                                   G GK+ +  D  G   L  AAR+G L  ++ L+ ++ +V     +  TALH AAA GH +VV+ L ++  I   +  G S L +A        V+  L      N+ + +   G      L++ ++
Sbjct:  470 LMKILQPQDVDLALDSG--ASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVE-----RRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNAS------VNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSD--GYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADVIDLFDEQGLSALHLAAQGRHAQTVETLLRHGAHINLQSLKFQGGHGPAATLLRRSK 831          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 126.331 bits (316), Expect = 8.616e-29
Identity = 90/341 (26.39%), Postives = 167/341 (48.97%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASII-----NYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL 1103
            +++ DN+G+  L  A    + +I++  LD   ++N +  DG+T    A++       +  +  L+  R   ++  D D  +PL  A     +D+V +LL+ GA+++  + + +TP+L A     A    + ++  L+   A ++  D + +T+      +  +E+++ + +   +       N +D    +PLH A   G+  IC+ L+E GA  N    +  +P  +A+++GH D V++LL +    +  R  +  +A+ +AA  G+ +I + L   G D   KD  GR TL   A    L + EY L    NV   D    TALH +  +GH+++VQ L
Sbjct:  491 VNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAA-----ASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE------NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNID-QRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVL 819          

HSP 2 Score: 110.538 bits (275), Expect = 5.515e-24
Identity = 80/306 (26.14%), Postives = 151/306 (49.35%), Query Frame = 3
Query:   93 ALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSR--KSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYL 1004
            A+D  D++G+  LS A    N+++++  LD  +D N +   G T  H A+   + ++ + +  +    N  DND   P   A      D V++LL+  +NI+    D +       + ++AL+    I++LL    AD+N +D D +   + L ++ ++ M ++  EN +       + + D   ++ LH +   G++E+ QVL+   A+ N         L  AA +GH+ +V+LL+         C   +  ++A+ IAA+ G+ ++ + L+  G D    D+FGR  +  AA++G   II+ L
Sbjct:  627 AVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRN-----ALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGAN------VEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTC---NQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 918          

HSP 3 Score: 88.9669 bits (219), Expect = 2.762e-17
Identity = 90/405 (22.22%), Postives = 172/405 (42.47%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLA----------------------------CMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYA-----VSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLND 1208
            ++ +D+   C +  A++ +  D I+  LD    +N   ++G+T+   A+      +  L+ SR  ++ + D   ++PL  A       +V  L+  GANIN  ++D  T +  A                             +  +A      I+  L+   A++N  D + +T           E+++ + ++ +E      +N +D+  ++ L  A      S G+  +  +L++ GA  +    +   PL +AA +GH+D+V LLL      +   D+N  + +  AA  G+  +   L+  G    + D  GR  L  A+  G + ++  LL +  + N  D    T LH AA EGH  + + L   GA  ++ +  G+ P  +A      + V+  L +
Sbjct:  328 VNSEDDRTSCIVRQALERE--DSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAE------VNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVD-HTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN 723          

HSP 4 Score: 84.3445 bits (207), Expect = 7.482e-16
Identity = 61/253 (24.11%), Postives = 123/253 (48.62%), Query Frame = 3
Query:   45 F*SSKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
            F   +LI E  I      +E DNDG+     A Q  + D ++I L+   +I+ +  DG+     A++  +  + +L+ S   ++N  D D    L+   + N+L M +  L+ GAN+   + + +T + ++C +         ++++LI   AD+N  D + ++       +  +++++ + E+ +      +++       + L  A   G++++ QVLLE GA+PN         + +AA+ GH  I+KLL
Sbjct:  677 FEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQ-----GHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGA------VVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 918          

HSP 5 Score: 79.337 bits (194), Expect = 2.110e-14
Identity = 79/294 (26.87%), Postives = 139/294 (47.28%), Query Frame = 3
Query:  339 IINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHD-----QMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFL 1202
            +I  + +++++L+K GA++N  ++D +T    +C+   AL+    I + L+   A +N  D + +T+         ++++  +   +S    +E+   +D    +PL  A   G+ ++   L+  GAN N    +    L  AA  GH ++V  LL +  K +C  D++  +A+  AA  G+ +I   L+  G +    D  GR  L+ AA  G   I+E+LL     VN  D      + V AL   A++GH  VV  L   GA +   +  G +PL +A      +VV   L
Sbjct:  310 LIPKEQEVLQLLVKAGAHVN--SEDDRT----SCIVRQALEREDSI-RTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLL---VSRGADLEI---EDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCA-DADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLL 589          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: trpa-1 (Transient receptor potential cation channel subfamily A member 1 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18297])

HSP 1 Score: 216.468 bits (550), Expect = 5.366e-58
Identity = 216/869 (24.86%), Postives = 376/869 (43.27%), Query Frame = 3
Query:   60 LIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADG-KTIFHTASIINYPILFKLMKSRDININVGDNDLNS----PLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKT----------------IFHYLVVKEK---IEMIKWIYEN----ISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG-AILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAY-------RHDNG-----SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLID---------------------AGPDDPASISE-------------RYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILIT---------YFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFACNSLALIII------SNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKN---------YYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQR 2360
            ++  + +    AL+  +N+ +  L  AV+  + + +K  L      + K  D  K + H A+   +  + K +    +      N+LN     PLH A   N+L++V  L++    NI++ ++   TP+++A    S        ++ LI +KA+L I D D +T                I  +L  K K      +K    N    +SE  R  ++N  D    +P+H   S G LE+ Q+L + GA+      ++   LH AA  G    V+ LL    +    +D   +SA+H+AA +G+    K L+  G D  AK+ + +  L  A  SG L   + L+ K   +      K T LH AA  G+  +V+     G  I   +  G++  DIA  N+ ++V + FL  D W+N+M         +H N       +    L+    +L   ++D CI E +  + D    V Y F  +D                     +  D+   + +             +YH LK M + ++L LL HPLS  LLK KW++ G   +     ++LV+I+ +T         Y V + E   DS+  D    C   N++           P+V   I+I  +   + ++    L  +KF  L  +E W +C+    S ALI +      S  S +   ++W      IF  +   ++ ++ +P  GI ++MF  ++KT  R    F      F+  FY+I Q++  F  + ++ +K+  MM GEF+    F   +           +   +  +   F +IM+I L NL++GLAV DI  +  +A ++   ++V  ++++E ++   IQR
Sbjct:  258 MMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKAL----VEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDS-----KKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTK-TVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCI-EKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTK----------PDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIY--STALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQR 1103          

HSP 2 Score: 103.605 bits (257), Expect = 2.831e-22
Identity = 115/508 (22.64%), Postives = 212/508 (41.73%), Query Frame = 3
Query:   93 ALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTAS--IINYPIL--------------FKLMKSRDININVGDN----DLN----SPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVK------------EKIEMIKWI-------------------YENISESQRIELINSQDIVDKSP--LHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL-----------------------------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLNDDNWQ-NVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPN 1352
            A++ +D D    L YA +  N D +K+ L      NTK  +G T  H AS  I  Y  +              +     + IN  V +N     +N    +PLH+A + +    +  L+K  A+++  + +  TP+LLAC+  S       II+ LI   +++  RD  + T+FH + ++            + +E IK +                    E + +  ++E  NS   +D+    +H+A   G LE+ + L+EAG N N       +PLH+AA+   L++V  L+   + +    D    + + +A  + + +  ++LI    +    D+  R  +   A+   L  +EY+L                              + T VN+ D+ + T +H  A+ G+L+++Q L   GA ++  N   ++ L  A    Q   V+  L    W   ++  + + G+S +    +S  D T     K +++N ++K   N
Sbjct:   75 AVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGS-----QEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLL---EWDIRLLLMKDEMGNSALHLAARSGHDATT----KVLLDNGADKEAKN 570          

HSP 3 Score: 76.2554 bits (186), Expect = 8.767e-14
Identity = 91/399 (22.81%), Postives = 162/399 (40.60%), Query Frame = 3
Query:  189 VDINTKQADGKTIFH-TASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNL-------------------SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGAN--------------------P------------------NIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKET-NVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            +D  T+  + ++I H +A   N   L + +    + +N  D D  +PLH+A      D VK+LL   A  N  N++  TP+ +A   I    ++                     II  L+ E A+++  +    T  HY  +K     +  + +  ++      ++++D    +PL  A   G+ EI Q L++A +N                    P                  N+F      PL +A E  H + +K +L   +K+ C     E   IH AAE G  E+ K L+  G +    +E     L  AA+   L ++ YL+ +E  N+++ D+  +T L  A      + V+ L    A L+  +   ++P+ I    N  + V++ L+
Sbjct:   40 LDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKAD------VDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILD 432          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: trpa-1 (Transient receptor potential cation channel subfamily A member 1 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q18297])

HSP 1 Score: 216.468 bits (550), Expect = 5.887e-58
Identity = 216/869 (24.86%), Postives = 376/869 (43.27%), Query Frame = 3
Query:   60 LIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADG-KTIFHTASIINYPILFKLMKSRDININVGDNDLNS----PLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKT----------------IFHYLVVKEK---IEMIKWIYEN----ISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG-AILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAY-------RHDNG-----SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLID---------------------AGPDDPASISE-------------RYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILIT---------YFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFACNSLALIII------SNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKN---------YYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQR 2360
            ++  + +    AL+  +N+ +  L  AV+  + + +K  L      + K  D  K + H A+   +  + K +    +      N+LN     PLH A   N+L++V  L++    NI++ ++   TP+++A    S        ++ LI +KA+L I D D +T                I  +L  K K      +K    N    +SE  R  ++N  D    +P+H   S G LE+ Q+L + GA+      ++   LH AA  G    V+ LL    +    +D   +SA+H+AA +G+    K L+  G D  AK+ + +  L  A  SG L   + L+ K   +      K T LH AA  G+  +V+     G  I   +  G++  DIA  N+ ++V + FL  D W+N+M         +H N       +    L+    +L   ++D CI E +  + D    V Y F  +D                     +  D+   + +             +YH LK M + ++L LL HPLS  LLK KW++ G   +     ++LV+I+ +T         Y V + E   DS+  D    C   N++           P+V   I+I  +   + ++    L  +KF  L  +E W +C+    S ALI +      S  S +   ++W      IF  +   ++ ++ +P  GI ++MF  ++KT  R    F      F+  FY+I Q++  F  + ++ +K+  MM GEF+    F   +           +   +  +   F +IM+I L NL++GLAV DI  +  +A ++   ++V  ++++E ++   IQR
Sbjct:  259 MMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKAL----VEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDS-----KKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTK-TVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCI-EKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTK----------PDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIY--STALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQR 1104          

HSP 2 Score: 103.605 bits (257), Expect = 3.221e-22
Identity = 115/508 (22.64%), Postives = 212/508 (41.73%), Query Frame = 3
Query:   93 ALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTAS--IINYPIL--------------FKLMKSRDININVGDN----DLN----SPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVK------------EKIEMIKWI-------------------YENISESQRIELINSQDIVDKSP--LHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL-----------------------------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLNDDNWQ-NVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPN 1352
            A++ +D D    L YA +  N D +K+ L      NTK  +G T  H AS  I  Y  +              +     + IN  V +N     +N    +PLH+A + +    +  L+K  A+++  + +  TP+LLAC+  S       II+ LI   +++  RD  + T+FH + ++            + +E IK +                    E + +  ++E  NS   +D+    +H+A   G LE+ + L+EAG N N       +PLH+AA+   L++V  L+   + +    D    + + +A  + + +  ++LI    +    D+  R  +   A+   L  +EY+L                              + T VN+ D+ + T +H  A+ G+L+++Q L   GA ++  N   ++ L  A    Q   V+  L    W   ++  + + G+S +    +S  D T     K +++N ++K   N
Sbjct:   76 AVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGS-----QEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLL---EWDIRLLLMKDEMGNSALHLAARSGHDATT----KVLLDNGADKEAKN 571          

HSP 3 Score: 75.8702 bits (185), Expect = 9.796e-14
Identity = 91/399 (22.81%), Postives = 162/399 (40.60%), Query Frame = 3
Query:  189 VDINTKQADGKTIFH-TASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNL-------------------SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGAN--------------------P------------------NIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKET-NVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            +D  T+  + ++I H +A   N   L + +    + +N  D D  +PLH+A      D VK+LL   A  N  N++  TP+ +A   I    ++                     II  L+ E A+++  +    T  HY  +K     +  + +  ++      ++++D    +PL  A   G+ EI Q L++A +N                    P                  N+F      PL +A E  H + +K +L   +K+ C     E   IH AAE G  E+ K L+  G +    +E     L  AA+   L ++ YL+ +E  N+++ D+  +T L  A      + V+ L    A L+  +   ++P+ I    N  + V++ L+
Sbjct:   41 LDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKAD------VDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILD 433          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 121.709 bits (304), Expect = 1.130e-27
Identity = 102/361 (28.25%), Postives = 168/361 (46.54%), Query Frame = 3
Query:  129 LSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLND 1208
            L  A +   + ++++ L +   I      G T  H A+ +    +   +  +  N +V      +PLH A   N+ D+V+VL++ GA ++   ++ QTP+ +A    S L N + I+ LL+   A+ N    D  +  H +  KE  E +  I   +  +    L+  +     +PLH A   GNLE+ ++LLE G   +I   N   PLH+AA   +  +  LLL +   ++     N  + +HIAA+    EI   L+    D  AK   G   L  +A+ G   I   L+   ++V       +TA+H  A E H+ V Q L+ +GA I S    G +PL +A    Q N+VKF + +
Sbjct:  396 LHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIA----SRLGN-TDIVILLLQAGANSNATTRDNYSPLH-IAAKEGQEEVAGIL--LDHNADKTLLTKKGF---TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKA-AAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 2 Score: 104.375 bits (259), Expect = 2.740e-22
Identity = 90/350 (25.71%), Postives = 158/350 (45.14%), Query Frame = 3
Query:  117 GQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNM-FGQSPLDIA 1163
            G+  L  A +    D++++ +     ++ +  + +T  H AS +    +  L+     N N    D  SPLH A    + ++  +LL   A+  L  K   TP+ LA    S   NL  +++LL+     ++I   +  T  H        ++   + EN   S +    N       +PLH A     +EI   LL+  A+PN  +     PLH++A++GH +I  LL+ +       + +N  +A+H+ A+  +  + + L   G +  +K   G   L  A   G L ++++L+    +V    +   T LHQAA +GH   V+ L  +GA  ++    GQ+PL IA
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLA----SKYGNLE-VVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN-GASAKAAAKNGY-----TPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGA-KANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795          

HSP 3 Score: 98.2117 bits (243), Expect = 2.211e-20
Identity = 98/379 (25.86%), Postives = 166/379 (43.80%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKS---PLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFL 1202
            IN  NA      +G   L  A +  + ++++  +     ++     G T  H AS+    ++  ++     N+NV   +  +PL+ A   N  ++VK LLK GAN  L  +D  TP+ +A  +         ++ +L+   +   +R        H    K+       + +N          ++ D+  KS   PLH A   G+  + Q+LLE GAN N  A ++  PLH+A + G  ++  LLL+     +  R  +  + +H AA +G+ ++   L+  G    AK + G   L  AA+   +     LL     V+      +T LH AA  GH++V + L    A   S  + G +PL IA   N+  VV+  L
Sbjct:   59 INTSNA------NGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQ-----GHDRVVAVLLENDSKGKVR----LPALHIAAKKDDTTAATLLLQN---------EHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDS-RTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 4 Score: 96.2857 bits (238), Expect = 7.763e-20
Identity = 89/344 (25.87%), Postives = 154/344 (44.77%), Query Frame = 3
Query:  192 DINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG----AILSDNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            D+ +K   G T  H A+   +  + +L+  +  N+N       SPLH A    + +M  +LL  GA I+   KD  TP  L C   +A      ++ LL+++ A ++ +  +     H     + ++  + +  + +       ++   +   +PLH A   G++ + ++LL+  A+PN  A N   PLHIA +K  + +V+LLL      E   +S   + +H+AA  G   I  +L+  G +   +   G   L  AAR+    ++  L+     V+   +   T LH A+  G+  +V  L  +G    A   DN    SPL IA    QE V    L+ +
Sbjct:  221 DVTSKS--GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTP--LHC---AARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAP------VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESG-LTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY---SPLHIAAKEGQEEVAGILLDHN 547          

HSP 5 Score: 90.1225 bits (222), Expect = 6.662e-18
Identity = 94/399 (23.56%), Postives = 165/399 (41.35%), Query Frame = 3
Query:   99 DEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLAC----------------------------MEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLND 1208
            D     G   L  A    + ++ ++ L+   ++N +     +  H A+      +  L+ SR   I+    DL +PLH A  +    +V +L+  GA I+   K+   P+ +A                             + ++A      + KLL+   AD N R  +  T  H    K +I++++ +   +     IE      +   +PLH A  +G + I   LL+ GANP++       PLH+AA     D+V++L+ +  K +  +     + +HIA+  GN +I   L+  G +  A      + L  AA+ G   +   LL    +  L  +   T LH A+  G+L+VV+ L   G  +      Q +PL +A   N + V    L +
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL---LKYRAAIEATTESGL---TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDA-QARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 6 Score: 53.5286 bits (127), Expect = 7.709e-7
Identity = 47/173 (27.17%), Postives = 80/173 (46.24%), Query Frame = 3
Query:  705 LEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG---AILSDNMFGQSPLDIAISNNQENVVKFFLNDDN 1214
            ++A A P    A        AA  G L+ V  LL +        ++N  +++H+A++ G+ E+ + LI       A    G   L  A+ +G   I+  L+    NVN+      T L+ AA E H +VV+ L   G   A+ +++ F  +PL +A+    + VV   L +D+
Sbjct:   20 VQAPAAPEPGRAEGSASFLRAARAGDLEKVLELLRAGTDINT-SNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGF--TPLAVALQQGHDRVVAVLLENDS 189          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 121.709 bits (304), Expect = 1.130e-27
Identity = 102/361 (28.25%), Postives = 168/361 (46.54%), Query Frame = 3
Query:  129 LSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLND 1208
            L  A +   + ++++ L +   I      G T  H A+ +    +   +  +  N +V      +PLH A   N+ D+V+VL++ GA ++   ++ QTP+ +A    S L N + I+ LL+   A+ N    D  +  H +  KE  E +  I   +  +    L+  +     +PLH A   GNLE+ ++LLE G   +I   N   PLH+AA   +  +  LLL +   ++     N  + +HIAA+    EI   L+    D  AK   G   L  +A+ G   I   L+   ++V       +TA+H  A E H+ V Q L+ +GA I S    G +PL +A    Q N+VKF + +
Sbjct:  396 LHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIA----SRLGN-TDIVILLLQAGANSNATTRDNYSPLH-IAAKEGQEEVAGIL--LDHNADKTLLTKKGF---TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKA-AAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 2 Score: 104.375 bits (259), Expect = 2.740e-22
Identity = 90/350 (25.71%), Postives = 158/350 (45.14%), Query Frame = 3
Query:  117 GQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNM-FGQSPLDIA 1163
            G+  L  A +    D++++ +     ++ +  + +T  H AS +    +  L+     N N    D  SPLH A    + ++  +LL   A+  L  K   TP+ LA    S   NL  +++LL+     ++I   +  T  H        ++   + EN   S +    N       +PLH A     +EI   LL+  A+PN  +     PLH++A++GH +I  LL+ +       + +N  +A+H+ A+  +  + + L   G +  +K   G   L  A   G L ++++L+    +V    +   T LHQAA +GH   V+ L  +GA  ++    GQ+PL IA
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLA----SKYGNLE-VVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN-GASAKAAAKNGY-----TPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGA-KANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795          

HSP 3 Score: 98.2117 bits (243), Expect = 2.211e-20
Identity = 98/379 (25.86%), Postives = 166/379 (43.80%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKS---PLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFL 1202
            IN  NA      +G   L  A +  + ++++  +     ++     G T  H AS+    ++  ++     N+NV   +  +PL+ A   N  ++VK LLK GAN  L  +D  TP+ +A  +         ++ +L+   +   +R        H    K+       + +N          ++ D+  KS   PLH A   G+  + Q+LLE GAN N  A ++  PLH+A + G  ++  LLL+     +  R  +  + +H AA +G+ ++   L+  G    AK + G   L  AA+   +     LL     V+      +T LH AA  GH++V + L    A   S  + G +PL IA   N+  VV+  L
Sbjct:   59 INTSNA------NGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQ-----GHDRVVAVLLENDSKGKVR----LPALHIAAKKDDTTAATLLLQN---------EHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDS-RTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 4 Score: 96.2857 bits (238), Expect = 7.763e-20
Identity = 89/344 (25.87%), Postives = 154/344 (44.77%), Query Frame = 3
Query:  192 DINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG----AILSDNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            D+ +K   G T  H A+   +  + +L+  +  N+N       SPLH A    + +M  +LL  GA I+   KD  TP  L C   +A      ++ LL+++ A ++ +  +     H     + ++  + +  + +       ++   +   +PLH A   G++ + ++LL+  A+PN  A N   PLHIA +K  + +V+LLL      E   +S   + +H+AA  G   I  +L+  G +   +   G   L  AAR+    ++  L+     V+   +   T LH A+  G+  +V  L  +G    A   DN    SPL IA    QE V    L+ +
Sbjct:  221 DVTSKS--GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTP--LHC---AARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAP------VDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESG-LTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY---SPLHIAAKEGQEEVAGILLDHN 547          

HSP 5 Score: 90.1225 bits (222), Expect = 6.662e-18
Identity = 94/399 (23.56%), Postives = 165/399 (41.35%), Query Frame = 3
Query:   99 DEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLAC----------------------------MEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLND 1208
            D     G   L  A    + ++ ++ L+   ++N +     +  H A+      +  L+ SR   I+    DL +PLH A  +    +V +L+  GA I+   K+   P+ +A                             + ++A      + KLL+   AD N R  +  T  H    K +I++++ +   +     IE      +   +PLH A  +G + I   LL+ GANP++       PLH+AA     D+V++L+ +  K +  +     + +HIA+  GN +I   L+  G +  A      + L  AA+ G   +   LL    +  L  +   T LH A+  G+L+VV+ L   G  +      Q +PL +A   N + V    L +
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL---LKYRAAIEATTESGL---TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDA-QARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN 612          

HSP 6 Score: 53.5286 bits (127), Expect = 7.709e-7
Identity = 47/173 (27.17%), Postives = 80/173 (46.24%), Query Frame = 3
Query:  705 LEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG---AILSDNMFGQSPLDIAISNNQENVVKFFLNDDN 1214
            ++A A P    A        AA  G L+ V  LL +        ++N  +++H+A++ G+ E+ + LI       A    G   L  A+ +G   I+  L+    NVN+      T L+ AA E H +VV+ L   G   A+ +++ F  +PL +A+    + VV   L +D+
Sbjct:   20 VQAPAAPEPGRAEGSASFLRAARAGDLEKVLELLRAGTDINT-SNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGF--TPLAVALQQGHDRVVAVLLENDS 189          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 120.939 bits (302), Expect = 1.716e-27
Identity = 103/366 (28.14%), Postives = 170/366 (46.45%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLND 1208
            +G   L  A +   + ++++ L +   I      G T  H A+ +    +   +  +  N +V      +PLH A   N+ D+V+VL++ GA ++   ++ QTP+ +A    S L N + I+ LL+   A+ N    D  +  H +  KE  E +  I   +  +    L+  +     +PLH A   GNLE+ ++LLE G   +I   N   PLH+AA   +  +  LLL +   ++     N  + +HIAA+    EI   L+    D  AK   G   L  +A+ G   I   L+   ++V       +TA+H  A E H+ V Q L+ +GA I S    G +PL +A    Q N+VKF + +
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIA----SRLGN-TDIVILLLQAGANSNATTRDNYSPLH-IAAKEGQEEVAGIL--LDHNADKTLLTKKGF---TPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAA-KNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVEN 744          

HSP 2 Score: 106.686 bits (265), Expect = 4.402e-23
Identity = 91/351 (25.93%), Postives = 159/351 (45.30%), Query Frame = 3
Query:  117 GQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES-SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNM-FGQSPLDIA 1163
            G+  L  A +    D++++ +     ++ +  + +T  H AS +    +  L+     N N    D  SPLH A    + ++  +LL   A+  L  K   TP+ LA    S   NL  +++LL+     ++I   +  T  H        ++   + EN   S +    N       +PLH A     +EI   LL+  A+PN  +     PLH++A++GH +I  LL+ +   S+    +N   +A+H+ A+  +  + + L   G +  +K   G   L  A   G L ++++L+    +V    +   T LHQAA +GH   V+ L  +GA  ++    GQ+PL IA
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLA----SKYGNLE-VVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN-GASAKAAAKNGY-----TPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENG--SDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795          

HSP 3 Score: 104.76 bits (260), Expect = 1.709e-22
Identity = 97/386 (25.13%), Postives = 165/386 (42.75%), Query Frame = 3
Query:   87 KNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR-DSNESS-----AIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLNDDNWQN 1223
            + A++     G   L  A     ++I+   L    + + +   G+T  H A+  N   + +++      ++    +L +PLH A      D+V +LL+ GAN N   +D  +P+ +A  E    + ++GI   L+   AD  +      T  H       +E+++ + E      R   ++ +     +PLH A    N ++  +LLE GA+    A N   PLHIAA+K  ++I   LL        F+ D N  S      +H++A+ G+ EI   LI  G D  AK   G   +   A+   +P+ + L      +N       T LH A   G L +V+ L  +GA + +      +PL  A      N V++ L +    N
Sbjct:  415 RAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKE--GQEEVAGI---LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE------RGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQ-------FKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPN 782          

HSP 4 Score: 98.5969 bits (244), Expect = 1.330e-20
Identity = 101/404 (25.00%), Postives = 175/404 (43.32%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQ-RIELI-------------------NSQDIVDKS---PLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNS-----SRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFL 1202
            +    A +    +G      A +  +L+ +   L    DINT  A+G    H AS   +  + + +  R   ++      N+ LH A +  +  +V +L++ GAN+N+ + +  TP+ +A  E     N   ++K L+   A+  +   D  T     + +    ++  + EN S+ + R+  +                   ++ D+  KS   PLH A   G+  + Q+LLE GAN N  A ++  PLH+A + G  ++  LLL+      SR  +        + +H AA +G+ ++   L+  G    AK + G   L  AA+   +     LL     V+      +T LH AA  GH++V + L    A   S  + G +PL IA   N+  VV+  L
Sbjct:   20 VQAPAAPEPGRAEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQE-----NHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLL------TPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 5 Score: 96.6709 bits (239), Expect = 4.309e-20
Identity = 91/343 (26.53%), Postives = 152/343 (44.31%), Query Frame = 3
Query:  192 DINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDK-SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVV----QCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            D+ +K   G T  H A+   +  + +L+  +  N+N       SPLH A    + +M  +LL  GA I+   KD  TP  L C   +A      ++ LL+++ A ++ +  +     H     + ++  + +  + +    +        VD  +PLH A   G++ + ++LL+  A+PN  A N   PLHIA +K  + +V+LLL      E   +S   + +H+AA  G   I  +L+  G +   +   G   L  AAR+    ++  L+     V+   +   T LH A+  G+  +V    Q    S A   DN    SPL IA    QE V    L+
Sbjct:  221 DVTSK--SGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTP--LHC---AARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------TVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESG-LTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY---SPLHIAAKEGQEEVAGILLD 545          

HSP 6 Score: 88.9669 bits (219), Expect = 1.007e-17
Identity = 106/455 (23.30%), Postives = 185/455 (40.66%), Query Frame = 3
Query:   99 DEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACM--EISALKNL--------------------------SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFS 1376
            D     G   L  A    + ++ ++ L+   ++N +     +  H A+      +  L+ SR   I+    DL +PLH A  +    +V +L+  GA I+   K+   P+ +A     + A + L                            + KLL+   AD N R  +  T  H    K +I++++ +   +     IE      +   +PLH A  +G + I   LL+ GANP++       PLH+AA     D+V++L+ +  K +  +     + +HIA+  GN +I   L+  G +  A      + L  AA+ G   +   LL    +  L  +   T LH A+  G+L+VV+ L   G  +      Q +PL +A   N + V    L +       A    NG + +    K N+   +E+    +      K DPN + +  F+
Sbjct:  221 DVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELL---LKYRAAIEATTESGL---TPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDA-QARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAA---KNGYTPLHIAAKKNQ---MEIASTLL----QFKADPNAKSRAGFT 658          

HSP 7 Score: 52.7582 bits (125), Expect = 1.307e-6
Identity = 48/177 (27.12%), Postives = 81/177 (45.76%), Query Frame = 3
Query:  705 LEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG---AILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNV 1226
            ++A A P    A        AA  G L+ V  LL +        ++N  +++H+A++ G+ E+ + LI       A    G   L  A+ +G   I+  L+    NVN+      T L+ AA E H +VV+ L   G   A+ +++ F  +PL +A+    + VV   L +D+   V
Sbjct:   20 VQAPAAPEPGRAEGSASFLRAARAGDLEKVLELLRAGTDINT-SNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGF--TPLAVALQQGHDRVVAVLLENDSKGKV 193          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: TrpA1 (gene:FBgn0035934 transcript:FBtr0331825)

HSP 1 Score: 253.832 bits (647), Expect = 2.861e-70
Identity = 225/858 (26.22%), Postives = 408/858 (47.55%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH------------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYF---VLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +    K   ++ ++Y F+ +   P   A I E+              AL +M  + R++LL HPLS K L++KW+ +G  F   + L++ ++++ +T +   +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  249 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQC-PFMFAKIDEKTGESITTASPIPLPALNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1096          

HSP 2 Score: 85.5001 bits (210), Expect = 1.333e-16
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   63 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 500          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: TrpA1 (gene:FBgn0035934 transcript:FBtr0331823)

HSP 1 Score: 253.447 bits (646), Expect = 4.549e-70
Identity = 225/858 (26.22%), Postives = 408/858 (47.55%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISER------------YHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYF---VLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +    K   ++ ++Y F+ +   P   A I E+              AL +M  + R++LL HPLS K L++KW+ +G  F   + L++ ++++ +T +   +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  284 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQC-PFMFAKIDEKTGESITTASPIPLPALNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1131          

HSP 2 Score: 85.5001 bits (210), Expect = 1.360e-16
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   98 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 535          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: TrpA1 (gene:FBgn0035934 transcript:FBtr0331824)

HSP 1 Score: 253.062 bits (645), Expect = 5.887e-70
Identity = 224/856 (26.17%), Postives = 403/856 (47.08%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----------DPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLL---FLVYIILITYFVLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +    K   ++ ++Y F      P+          DP         + +M  + R++LL HPLS K L++KW+ +G  F   + L+   FLV++ + +  +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  284 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFWPYQKTPEQIEAKRKEFNDPKWRPAPLAVVNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1130          

HSP 2 Score: 85.8853 bits (211), Expect = 1.062e-16
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   98 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 535          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: TrpA1 (gene:FBgn0035934 transcript:FBtr0331826)

HSP 1 Score: 252.292 bits (643), Expect = 8.544e-70
Identity = 222/856 (25.93%), Postives = 404/856 (47.20%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----------DPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYF---VLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +    K   ++ ++Y F      P+          DP         + +M  + R++LL HPLS K L++KW+ +G  F   + L++ ++++ +T +   +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  249 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFWPYQKTPEQIEAKRKEFNDPKWRPAPLAVVNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1095          

HSP 2 Score: 85.5001 bits (210), Expect = 1.553e-16
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   63 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 500          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Match: TrpA1 (gene:FBgn0035934 transcript:FBtr0331827)

HSP 1 Score: 239.58 bits (610), Expect = 1.663e-65
Identity = 219/846 (25.89%), Postives = 395/846 (46.69%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLL---FLVYIILITYFVLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +                          A+  +   +  +M  + R++LL HPLS K L++KW+ +G  F   + L+   FLV++ + +  +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  284 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITK--------------------------ANCKKDSKSFYTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1094          

HSP 2 Score: 85.5001 bits (210), Expect = 1.355e-16
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   98 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 535          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: trpa1a (transient receptor potential cation channel, subfamily A, member 1a [Source:ZFIN;Acc:ZDB-GENE-050105-6])

HSP 1 Score: 208.764 bits (530), Expect = 2.476e-55
Identity = 200/831 (24.07%), Postives = 378/831 (45.49%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSR-----DININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRF--------SLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNC-----YFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEF----DLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQ---HKKRFIVSWLFQNSFIFTKSEF 2465
            +E G+++   ++  D      L  AV+  NLDIIK+C+ +   I+ +Q D  T  H A       + K+M S      D+ IN+ D    +PLH A+I +  ++ + L+  GANI+  +    +P+LLA     A +     + LL+   ADL  +D       H  +++ +   +K +   + + + + EL+N +DI   +PLHYA  +G  +  + +L    + +  +      LH AAE G ++    LL     +    + +E   + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    ++ LLT    + N  D    TALH AA  GH+  V+ L   GA +  N    S L  A+ N +  V    +  D  +  M     N +    + ++I+   +    LLD CI E+  +    NY ++Y F        +L   G +   +      AL +M  + R+ LL HP+  K L++KW  +G+    ++  ++ + +  +TY +++    L    V       +N   T   ++   +    TS++L+++ +   N+  V   I+Q F+    +  +      + A     L ++     L   + W+        S+   + YLQ    +GI ++MF+++ +T++ ++  FF +  GFA  FY +  +Q  F  + L+ +++  MM GE     + +  ++ G   +   +L +   FV+++ I L NL+IGLAVGDI ++ + A ++   +++     +E  +P    + +    I + P +    KKR    W F  + + +++  
Sbjct:  231 EEAGLSIDAHINYVDKSCSSPLHLAVRGGNLDIIKLCIGYGAKIDQQQCDKSTALHFACSQGATEVVKIMLSSYPKVCDL-INITDGANQTPLHKAVIFDHFELSEYLMSQGANIDFVDCKGHSPLLLAT-SCGAWRT----VNLLLSHGADLTKKDKSGCNFLHLAILQPR--GLKNLPTEVLQHESVRELLNDEDIEGCTPLHYACRLGIPDSVKNMLGLEVSLDQKSKEKKSALHFAAEFGRINTCHRLLEMVTDTRLLNEGDEKGLTPLHLASREGHVKVVELLLRKGA-LFHSDYRGWSGLHHAASEGYTQTMDTLLTSNIKLLNKTDGDGNTALHLAARAGHVAAVRLLLYRGAKIILNKNDASFLHEAVHNARREVTNMVIESDRCEEAMTTYKPNSTKRCIVMDMIEFLPESFKHLLDTCIRESEEDVNCTNYYIEYNFRWLQHPLQNLKKTGMEKDMAYKP-LSALNAMVNFNRVNLLTHPVCKKYLEMKWSAYGIKAHLLNMTVYALGVFPLTYLIVN----LKPTLVTSRNVTSVNMVCTSLYKQSYLT----TSSMLLVLAM---NMYAVGKEILQMFQQRLNYLRDLSNYMDWAAAICALLFVVPLLMNLKSSWHWQAGALAALTSWLNLLLYLQRFERIGIYVVMFREISRTLLSIIVLFFYLILGFALSFYALMIEQQHFGRMFLSLLQTFVMMVGEMNYQDNFMKPYLQGDLPFPDLTLAIFVWFVLLVPILLMNLLIGLAVGDIAEVQTNACLKRIAMQIELHTNLEERLPYWFMKRVDQVTIREYPNRCFSGKKR----WFFGGNEVKSRTRL 1036          

HSP 2 Score: 73.1738 bits (178), Expect = 1.221e-12
Identity = 91/357 (25.49%), Postives = 152/357 (42.58%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEK-ADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLL----EAG----ANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-GIGKDCLA---KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS 1127
            LD +DN G   L YA  N +  II+                    H   I+ +             +NV D + N+PLH A+  ++     VLL  GA+ N+ N   Q PI +A   +S  KN   +++ L+  K  D+N+      T        +  E +  +Y++ ++  R      Q+ +   P+H A   G  +  +V+L    EAG    A+ N    +   PLH+A   G+LDI+KL +    K +  +  ++S+A+H A   G  E+ K ++    K C      D   +  L  A       + EYL+++  N++  D    + L  A + G  + V  L + GA L+
Sbjct:   65 LDTRDNIGASPLHYASANGHFRIIR--------------------HIVQIVGHQ-----------ELNVRDEEGNTPLHWAVQKDQPGSCSVLLSLGADPNVLNNSHQAPIHMA---VSLGKNF--VLEQLVSHKQTDVNLEGDLGNTPVILSAALDNHEALGILYKHGAKFCR------QNNLGHFPIHAAAFSGAKKSMEVILLKGEEAGLSIDAHINYVDKSCSSPLHLAVRGGNLDIIKLCIGYGAKIDQ-QQCDKSTALHFACSQGATEVVKIMLSSYPKVCDLINITDGANQTPLHKAVIFDHFELSEYLMSQGANIDFVDCKGHSPLLLATSCGAWRTVNLLLSHGADLT 378          

HSP 3 Score: 63.1586 bits (152), Expect = 1.334e-9
Identity = 57/242 (23.55%), Postives = 102/242 (42.15%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFLN 1205
            L+ RD    +  HY        +I+ I + +   +    +N +D    +PLH+AV       C VLL  GA+PN+   +   P+H+A   G   +++ L++  +          ++ + ++A   N+E    L   G     ++  G   +  AA SG    +E +L K        + ++N  D+   + LH A   G+L +++     GA +      +S  L  A S     VVK  L+
Sbjct:   65 LDTRDNIGASPLHYASANGHFRIIRHIVQIVGHQE----LNVRDEEGNTPLHWAVQKDQPGSCSVLLSLGADPNVLNNSHQAPIHMAVSLGKNFVLEQLVSHKQTDVNLEGDLGNTPVILSAALDNHEALGILYKHGAKFCRQNNLGHFPIHAAAFSGAKKSMEVILLKGEEAGLSIDAHINYVDKSCSSPLHLAVRGGNLDIIKLCIGYGAKIDQQQCDKSTALHFACSQGATEVVKIMLS 302          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: trpa1b (transient receptor potential cation channel, subfamily A, member 1b [Source:ZFIN;Acc:ZDB-GENE-050106-1])

HSP 1 Score: 205.297 bits (521), Expect = 3.233e-54
Identity = 190/796 (23.87%), Postives = 359/796 (45.10%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCL---------PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNC--YFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            K+ +E G++    ++  D      L  AV+  N+++IK+C+     +   Q+   T  H A         K+M     ++ DI +N+ D    +PLH A + + +++ + L+  GA I+  +    +P+LLA    SA K ++ ++ +     AD  I++   +   H+++++ K   +K + E + +S  + E+++ +D+   +PLHYA  +G  +  +         N+   N CL          LH AAE G ++    LL +   S+   D +E   + +H+A+  G+ ++   L+  G      D  G   L  AA  G    ++ LL    NV L D+      TALH AA  GH+  V  L   GA ++ N    S L  A+ N +  VV   +  +     M          + ++I+   +    LLD+CI E+  +    +Y + Y F  + A          D     +   AL +M  Y RL+LL HPLS K L++KW  +G     + FL   +Y++ +         +  S       G  +       SE++      ++  I++++++ + +I   +  + Q+  N F  ++N   + A  S  + ++     +   ++WE     I  S+  F+ Y Q    +GI ++MF  +++T++ ++  F  +   F   FY +   +  F ++ LA  ++  M  GE +  + F++    G   +   + ++   FV++M I L NL+IGLAVGDI ++   A ++   +++     +E  +P
Sbjct:  215 KIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDI-VNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLAS-SCSAWKTVAYLLSI----GADFKIKEKTGRNFLHFVILQPK--GLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVK---------NMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGA-LFQSDYKGWTCLHHAAAEGYTQTMKILLA--ANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGS---KVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYL----ISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLP 983          

HSP 2 Score: 88.9669 bits (219), Expect = 1.605e-17
Identity = 89/360 (24.72%), Postives = 151/360 (41.94%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTP--------------ILLACMEISALKNLSG-----------------IIKLLIIEKADLNIRD----YDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D +G   L YA    NLDII++ +                    SI+   +L           NV D    +PLH A+  ++     VLL  GA+ N+ N     P              +LL+C + ++  NL G                  +++LI   A + I++    Y + T+  +   KE +EM+  I E +  S  +  IN  D    +PLH AV  GN+E+ ++ +  GA      +  C  LH A  +G L+ VK++L+S  ++E     RD    + +H A    + E+ ++LI  G +  + D  G + L+ A+       + YLL+   +  + ++     LH
Sbjct:   52 LSLRDENGASLLHYASAGGNLDIIRLIV--------------------SIVGPEVL-----------NVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNS--NLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVA-FAGAKEAMEMVLKIGEELGVSSTLH-INYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLH 376          

HSP 3 Score: 86.2705 bits (212), Expect = 1.125e-16
Identity = 65/258 (25.19%), Postives = 122/258 (47.29%), Query Frame = 3
Query:  468 SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLNDDN 1214
            S I +L+      L++RD +  ++ HY      +++I+ I   +      E++N QD   ++PLH+AV     + C VLL+ GA+PNI       PLH+A  K +  + ++LL+  + +        ++ + +A  N N +  + LI  G     +++ G    +T+ FA     + ++     E  ++   ++N  D+ K T LH A   G+++V++     GA +  +  G+ + L  A S      VK  L+  N
Sbjct:   39 SAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGP----EVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYN 292          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: trpa1b (transient receptor potential cation channel, subfamily A, member 1b [Source:ZFIN;Acc:ZDB-GENE-050106-1])

HSP 1 Score: 205.297 bits (521), Expect = 3.233e-54
Identity = 190/796 (23.87%), Postives = 359/796 (45.10%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCL---------PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNC--YFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            K+ +E G++    ++  D      L  AV+  N+++IK+C+     +   Q+   T  H A         K+M     ++ DI +N+ D    +PLH A + + +++ + L+  GA I+  +    +P+LLA    SA K ++ ++ +     AD  I++   +   H+++++ K   +K + E + +S  + E+++ +D+   +PLHYA  +G  +  +         N+   N CL          LH AAE G ++    LL +   S+   D +E   + +H+A+  G+ ++   L+  G      D  G   L  AA  G    ++ LL    NV L D+      TALH AA  GH+  V  L   GA ++ N    S L  A+ N +  VV   +  +     M          + ++I+   +    LLD+CI E+  +    +Y + Y F  + A          D     +   AL +M  Y RL+LL HPLS K L++KW  +G     + FL   +Y++ +         +  S       G  +       SE++      ++  I++++++ + +I   +  + Q+  N F  ++N   + A  S  + ++     +   ++WE     I  S+  F+ Y Q    +GI ++MF  +++T++ ++  F  +   F   FY +   +  F ++ LA  ++  M  GE +  + F++    G   +   + ++   FV++M I L NL+IGLAVGDI ++   A ++   +++     +E  +P
Sbjct:  215 KIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDI-VNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLAS-SCSAWKTVAYLLSI----GADFKIKEKTGRNFLHFVILQPK--GLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVK---------NMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGA-LFQSDYKGWTCLHHAAAEGYTQTMKILLA--ANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGS---KVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYL----ISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLP 983          

HSP 2 Score: 88.9669 bits (219), Expect = 1.605e-17
Identity = 89/360 (24.72%), Postives = 151/360 (41.94%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTP--------------ILLACMEISALKNLSG-----------------IIKLLIIEKADLNIRD----YDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D +G   L YA    NLDII++ +                    SI+   +L           NV D    +PLH A+  ++     VLL  GA+ N+ N     P              +LL+C + ++  NL G                  +++LI   A + I++    Y + T+  +   KE +EM+  I E +  S  +  IN  D    +PLH AV  GN+E+ ++ +  GA      +  C  LH A  +G L+ VK++L+S  ++E     RD    + +H A    + E+ ++LI  G +  + D  G + L+ A+       + YLL+   +  + ++     LH
Sbjct:   52 LSLRDENGASLLHYASAGGNLDIIRLIV--------------------SIVGPEVL-----------NVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNS--NLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVA-FAGAKEAMEMVLKIGEELGVSSTLH-INYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLH 376          

HSP 3 Score: 86.2705 bits (212), Expect = 1.125e-16
Identity = 65/258 (25.19%), Postives = 122/258 (47.29%), Query Frame = 3
Query:  468 SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLNDDN 1214
            S I +L+      L++RD +  ++ HY      +++I+ I   +      E++N QD   ++PLH+AV     + C VLL+ GA+PNI       PLH+A  K +  + ++LL+  + +        ++ + +A  N N +  + LI  G     +++ G    +T+ FA     + ++     E  ++   ++N  D+ K T LH A   G+++V++     GA +  +  G+ + L  A S      VK  L+  N
Sbjct:   39 SAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGP----EVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYN 292          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: trpa1b (transient receptor potential cation channel, subfamily A, member 1b [Source:ZFIN;Acc:ZDB-GENE-050106-1])

HSP 1 Score: 197.208 bits (500), Expect = 1.555e-51
Identity = 191/809 (23.61%), Postives = 359/809 (44.38%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVL-------------LKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCL---------PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNC--YFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            K+ +E G++    ++  D      L  AV+  N+++IK+C+     +   Q+   T  H A         K+M     ++ DI +N+ D    +PLH A + + +++ + L             L  GA I+  +    +P+LLA    SA K ++ ++ +     AD  I++   +   H+++++ K   +K + E + +S  + E+++ +D+   +PLHYA  +G  +  +         N+   N CL          LH AAE G ++    LL +   S+   D +E   + +H+A+  G+ ++   L+  G      D  G   L  AA  G    ++ LL    NV L D+      TALH AA  GH+  V  L   GA ++ N    S L  A+ N +  VV   +  +     M          + ++I+   +    LLD+CI E+  +    +Y + Y F  + A          D     +   AL +M  Y RL+LL HPLS K L++KW  +G     + FL   +Y++ +         +  S       G  +       SE++      ++  I++++++ + +I   +  + Q+  N F  ++N   + A  S  + ++     +   ++WE     I  S+  F+ Y Q    +GI ++MF  +++T++ ++  F  +   F   FY +   +  F ++ LA  ++  M  GE +  + F++    G   +   + ++   FV++M I L NL+IGLAVGDI ++   A ++   +++     +E  +P
Sbjct:  215 KIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDI-VNIRDGANRTPLHRATLFDHVELAEYLISKPNTLSLDLICLGKGAEIDSIDCKGLSPLLLAS-SCSAWKTVAYLLSI----GADFKIKEKTGRNFLHFVILQPK--GLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVK---------NMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGA-LFQSDYKGWTCLHHAAAEGYTQTMKILLA--ANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGS---KVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYL----ISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLP 996          

HSP 2 Score: 86.6557 bits (213), Expect = 9.136e-17
Identity = 65/258 (25.19%), Postives = 122/258 (47.29%), Query Frame = 3
Query:  468 SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLNDDN 1214
            S I +L+      L++RD +  ++ HY      +++I+ I   +      E++N QD   ++PLH+AV     + C VLL+ GA+PNI       PLH+A  K +  + ++LL+  + +        ++ + +A  N N +  + LI  G     +++ G    +T+ FA     + ++     E  ++   ++N  D+ K T LH A   G+++V++     GA +  +  G+ + L  A S      VK  L+  N
Sbjct:   39 SAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGP----EVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYN 292          

HSP 3 Score: 82.4185 bits (202), Expect = 1.662e-15
Identity = 90/373 (24.13%), Postives = 152/373 (40.75%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTP--------------ILLACMEISALKNLSG-----------------IIKLLIIEKADLNIRD----YDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLI-------------GIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D +G   L YA    NLDII++ +                    SI+   +L           NV D    +PLH A+  ++     VLL  GA+ N+ N     P              +LL+C + ++  NL G                  +++LI   A + I++    Y + T+  +   KE +EM+  I E +  S  +  IN  D    +PLH AV  GN+E+ ++ +  GA      +  C  LH A  +G L+ VK++L+S  ++E     RD    + +H A    + E+ ++LI             G G +  + D  G + L+ A+       + YLL+   +  + ++     LH
Sbjct:   52 LSLRDENGASLLHYASAGGNLDIIRLIV--------------------SIVGPEVL-----------NVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNS--NLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVA-FAGAKEAMEMVLKIGEELGVSSTLH-INYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKPNTLSLDLICLGKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLH 389          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Match: ripk4 (receptor-interacting serine-threonine kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040426-2042])

HSP 1 Score: 130.954 bits (328), Expect = 1.186e-30
Identity = 93/338 (27.51%), Postives = 161/338 (47.63%), Query Frame = 3
Query:  111 NDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDI-NINVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            + G   L YAV   N + +K  L    + N   A G T  H A+      + +++ SR   N+N  D D  +PLH A  N    + ++LL   A+IN  +   +TP  +AC           ++++L+   AD++++  D  T  H    K  + ++K + +          ++ Q    +SPLH A   G   + ++L+E GAN ++ + +   PLH+AAE GH    +LL+      +  R +N  +A+H+A++ G+    K L+  G D  + +   R     AA++G   +++ LL   ++V N  D+  +TALH A + GH   +  L   GA
Sbjct:  447 DGGSNLLHYAVSLANEEAVKFLLLSNCNPNLANAQGATPLHQAAEKRLKGVSEILLSRKTTNVNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHH-----GQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGAD-----VDGQTSDGRSPLHLASQRGQYRVARILVELGANVHLTSDDLYAPLHVAAETGHTSTSRLLVKHDADIKS-RTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGA 773          

HSP 2 Score: 115.161 bits (287), Expect = 1.079e-25
Identity = 92/319 (28.84%), Postives = 153/319 (47.96%), Query Frame = 3
Query:  261 LFKLMKSRDININV-GDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKA-DLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDC-LAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            L K+++ +D+++ + G ++L   LH+A+     + VK LL    N NL N    TP+  A     A K L G+ ++L+  K  ++N +D D  T  H+        + + + +      R   IN  D   ++P H A   G   + +VLL  GA+ ++   +D   LH+AA KGHL IVKLL+  +      + S+  S +H+A++ G Y + + L+ +G +  L  D+     L  AA +G       L+  + ++        TALH A+ +GHL  V+ L   GA   S N   ++P  +A  N    V+K  L 
Sbjct:  433 LMKILQPQDVDLLLDGGSNL---LHYAVSLANEEAVKFLLLSNCNPNLANAQGATPLHQA-----AEKRLKGVSEILLSRKTTNVNAKDEDQYTPLHFAAQNGDEALTRLLLD------RSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQRGQYRVARILVELGANVHLTSDDL-YAPLHVAAETGHTSTSRLLVKHDADIKSRTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCEVLKELLR 736          

HSP 3 Score: 91.6633 bits (226), Expect = 2.210e-18
Identity = 72/297 (24.24%), Postives = 135/297 (45.45%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLK-FGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDC--LAKDEFGRNTLMFAARS 977
            ++ KD D    L +A QN +  + ++ LD    IN   A G+T  H A       + +++ SR  +++V   D  + LH A     L +VK+L+K  GA+++    D ++P+ LA       +    + ++L+   A++++   D+    H           + + ++ ++      I S+     + LH A   G+L   ++LL  GA+P     +   P H+AA+ GH +++K LL S       +D N  +A+H+A   G+ +    L+  G D   L     G  +  + + S
Sbjct:  509 VNAKDEDQYTPLHFAAQNGDEALTRLLLDRSASINETDAQGRTPTHIACHHGQENVVRVLLSRGADVHVKGKDDWTALHLAAWKGHLGIVKLLVKQAGADVDGQTSDGRSPLHLASQ-----RGQYRVARILVELGANVHLTSDDLYAPLHVAAETGHTSTSRLLVKHDAD------IKSRTANGCTALHLASQKGHLPTVKMLLAEGADPESVNHDLRTPCHLAAQNGHCEVLKELLRSCSDVANAQDRNGLTALHLAVSGGHKDAICVLLEGGADAAPLTPQPVGDRSFDYTSES 794          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:NCBI gene;Acc:100158526])

HSP 1 Score: 228.409 bits (581), Expect = 9.180e-62
Identity = 215/798 (26.94%), Postives = 385/798 (48.25%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKS--RDIN--INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIF--AANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNL---HDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH-----------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERI--LDSDSVDVETG---C----KINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G  + + ++  +N+    L  AVQN N+D++K C+ +   I+ +Q+D  T  H A+      + K M S   D N  +++ D +  +PLH +++ + +++ + L+  GANI+  +K+ +TP+LLA    SA K+    + LL+ + AD+ ++D+  +   H +V++     +K +     + + I +L++ +D    +PLHYA   G       LL  G N +++  + N   PLH AA  G  +  + LL          D +E   + +H+AAENG+ +I  FL+      L  D  G   L +AA SG    I+ LL  ET++ L    D+ + TA+H A  EGH + V  L  +GA +  N  G S +  AI   +++ V   +  + W+ ++  + H +     I  +I    +    LLD+C+ E+  +K  P++ V+Y F+ +      P S  + +             L +M +++R+ LL HP+  + L +KW  +G     ++  ++ + +I +T  +++  R   LD+    V  G   C     I   + Q  ++K A   +  SNI+   I  I +II V +L                  C+S   I++S         +W+     +F S+  F+ YLQ   S GI ++MF ++L+T++R++F FF +   F   FY+    Q  F     A M+S  MM G+ +    F+      +  Y   + I + +F +++ I L NL+IGLAVGDI ++   A ++   ++V     +E  +P
Sbjct:  183 EEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLA-TSCSAWKS----VNLLLSKGADVKLKDHYGRNFLHLMVLQPG--GLKNLNSEFLQMENIKKLVSDEDCEGCTPLHYACRHGVPNSVNNLL--GLNMSLYSKSKNKRSPLHFAACYGRFNTCQRLLRFMSDGRLLNDGDEKGMTPLHLAAENGHEKIV-FLLLKRGALLLSDHRGWTALHYAALSGYTRTIKTLL--ETSIGLIDKTDKEQNTAMHLAGREGHAKAVSLLLENGASILLNANGASYIHEAIRFGKKDTVLVSIQSERWEEILQTFSHTSSYKCAILEMIYHLPESFKTLLDRCMTESIGDKKSPDFHVEYNFTYLQC----PLSFRKAHKRENNVHYEPLTTLNAMVQHQRIDLLSHPVCKEYLLMKWMAYGFKAHLLNLAIYSLGLIPLTLLIINAVRPESLDTQYTRVCMGLVFCMSLFGIVKEAVQLCQQKLAYIGD-GSNIIDWSIY-ISSIIFVSSL------------------CHS--PIVVSG-------LQWQFGAIAVFASWVNFLIYLQRFESYGIYIVMFWEILRTLLRIVFLFFFLILAFGLSFYVFLYPQVTFSRPYFALMQSFTMMLGDINYEEGFLKPLLDDQIEYPFFTFIHLIMFTLLIPILLMNLLIGLAVGDIAEVQRNACLKRIAMQVSLHTSLEKKLP 935          

HSP 2 Score: 71.2478 bits (173), Expect = 7.061e-12
Identity = 72/311 (23.15%), Postives = 128/311 (41.16%), Query Frame = 3
Query:  441 MEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNI------------------------------FAAN--------------DCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETN----VNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            +E+ A  N+  +   ++     L  RD    T  H+      I+++  I    +E    E IN+ D    +PLH+AV    L+  +VLL  GA+PNI                              F+                 C P+H+ A  G L  + ++L    +          F ++ +SS +H+A +NGN ++ K  I  G     +       L FAA  G   I++++++  ++    V+L D    T LH++    H+++ + L + GA I S++   ++PL +A S +    V   L+
Sbjct:   18 LELIAEGNVCRVRNFVLKNPGSLAARDDSEATPLHHAAKLGNIDLMLMIVNESAE----EGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFHNSIAEVRFFQGICFSLRTLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLS 324          

HSP 3 Score: 64.6994 bits (156), Expect = 6.956e-10
Identity = 70/292 (23.97%), Postives = 130/292 (44.52%), Query Frame = 3
Query:  306 DNDLNSPLHHAIINNKLDMVKVLLKFGA--NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRD-YDMKT------IFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNI-------FAAND-CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKD----CLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            D+   +PLHHA     +D++ +++   A   IN  +    TP+  A       KN    +K+L+   AD NI++ Y +        +FH  +   ++   + I  ++    R   +  Q+ +   P+H    +G+L+   ++L+ G            F  N+   PLH+A + G++D+VK  ++   K +  R S+ ++A+H AA  G  EI KF++    D        D      L  +     + + EYL+++  N++  D+   T L  A +    + V  L + GA
Sbjct:   44 DDSEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQ-----KNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFHNSIA--EVRFFQGICFSLRTLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKID-LRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLSKGA 327          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:NCBI gene;Acc:100158526])

HSP 1 Score: 228.024 bits (580), Expect = 2.179e-61
Identity = 209/811 (25.77%), Postives = 388/811 (47.84%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSR--DIN--INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIE-LINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAA--NDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNL---HDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH-----------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCK-INNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSL------------------ALIIISNPSKLPCL---YEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G  + + ++  +N+    L  AVQN N+D++K C+ +   I+ +Q+D  T  H A+      + K M S   D N  +++ D +  +PLH +++ + +++ + L+  GANI+  +K+ +TP+LLA    SA K+    + LL+ + AD+ ++D+  +   H +V++     +K +     + + I+ L++ +D    +PLHYA   G       LL  G N ++++   N   PLH AA  G  +  + LL          D +E   + +H+AAENG+ +I  FL+      L  D  G   L +AA SG    I+ LL  ET++ L    D+ + TA+H A  EGH + V  L  +GA +  N  G S +  AI   +++ V   +  + W+ ++  + H +     I  +I    +    LLD+C+ E+  +K  P++ V+Y F+ +      P S  + +             L +M +++R+ LL HP+  + L +KW  +G     ++  ++ + +I +T  +++  R    +S+ +   C+ +  +   NS  K   A NV+  + ++ +   +   + M L+     +LF         C                     ++I +S+    P +    +W+     +F S+  F+ YLQ   S GI ++MF ++L+T++R++F FF +   F   FY+    Q  F     A M+S  MM G+ +    F+      +  Y   + I + +F +++ I L NL+IGLAVGDI ++   A ++   ++V     +E  +P
Sbjct:  228 EEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLA-TSCSAWKS----VNLLLSKGADVKLKDHYGRNFLHLMVLQPG--GLKNLNSEFLQMENIKKLVSDEDCEGCTPLHYACRHGVPNSVNNLL--GLNMSLYSKSKNKRSPLHFAACYGRFNTCQRLLRFMSDGRLLNDGDEKGMTPLHLAAENGHEKIV-FLLLKRGALLLSDHRGWTALHYAALSGYTRTIKTLL--ETSIGLIDKTDKEQNTAMHLAGREGHAKAVSLLLENGASILLNANGASYIHEAIRFGKKDTVLVSIQSERWEEILQTFSHTSSYKCAILEMIYHLPESFKTLLDRCMTESIGDKKSPDFHVEYNFTYLQC----PLSFRKAHKRENNVHYEPLTTLNAMVQHQRIDLLSHPVCKEYLLMKWMAYGFKAHLLNLAIYSLGLIPLTLLIINAVR---PESLAIIEECEHLKRTDFYNSSMKQECA-NVSRKLDLLQLKDTQYTRVCMGLVF--CMSLFGIVKEAVQLCQQKLAYIGDGSNIIDWSIYISSIIFVSSLCHSPIVVSGLQWQFGAIAVFASWVNFLIYLQRFESYGIYIVMFWEILRTLLRIVFLFFFLILAFGLSFYVFLYPQVTFSRPYFALMQSFTMMLGDINYEEGFLKPLLDDQIEYPFFTFIHLIMFTLLIPILLMNLLIGLAVGDIAEVQRNACLKRIAMQVSLHTSLEKKLP 1016          

HSP 2 Score: 78.9518 bits (193), Expect = 3.164e-14
Identity = 82/348 (23.56%), Postives = 148/348 (42.53%), Query Frame = 3
Query:  210 ADGKTIFHTASIINYPILFKLM-KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISES---QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETN----VNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            ++   + H A + N  ++  ++ +S +  IN  D+  N+PLH A+  NKLD +KVLL  GA+ N+ N    +P+ LA                                              I+  + +I+E+        IN +  +  +P+  A    N E   +LL  GA         C P+H+ A  G L  + ++L    +          F ++ +SS +H+A +NGN ++ K  I  G     +       L FAA  G   I++++++  ++    V+L D    T LH++    H+++ + L + GA I S++   ++PL +A S +    V   L+
Sbjct:   68 SEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLA----------------------------------------------IQLFHNSIAEALILHSTTDINLEGDLGNTPVMQACCKDNHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLS 369          

HSP 3 Score: 71.633 bits (174), Expect = 5.475e-12
Identity = 76/297 (25.59%), Postives = 130/297 (43.77%), Query Frame = 3
Query:  369 VLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL----SDNMFGQSPLDIAISNNQENVVKFFL---NDDN 1214
            V L+ G+   +C+ D   P+L+   ++ A  N+  +   ++     L  RD    T  H+      I+++  I    +E    E IN+ D    +PLH+AV    L+  +VLL  GA+PNI       PLH+A +  H  I + L+  S           ++ +  A    N+E    L+G G     +++ G   +   A  G L  ++ +L K        + ++N  +  K + LH A   G++ VV+   + GA +    SDN    + L  A +     +VKF +   +DDN
Sbjct:   22 VFLQTGS---VCSLD-DNPLLI--FKLIAEGNVCRVRNFVLKNPGSLAARDDSEATPLHHAAKLGNIDLMLMIVNESAE----EGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFHNSIAEALILHSTTDINLEGDLGNTPVMQACCKDNHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDN---ATALHFAATQGATEIVKFMVSSYSDDN 305          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:NCBI gene;Acc:100158526])

HSP 1 Score: 226.483 bits (576), Expect = 4.915e-61
Identity = 210/789 (26.62%), Postives = 382/789 (48.42%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKS--RDIN--INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIF--AANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNL---HDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH-----------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G  + + ++  +N+    L  AVQN N+D++K C+ +   I+ +Q+D  T  H A+      + K M S   D N  +++ D +  +PLH +++ + +++ + L+  GANI+  +K+ +TP+LLA    SA K+    + LL+ + AD+ ++D+  +   H +V++     +K +     + + I +L++ +D    +PLHYA   G       LL  G N +++  + N   PLH AA  G  +  + LL          D +E   + +H+AAENG+ +I  FL+      L  D  G   L +AA SG    I+ LL  ET++ L    D+ + TA+H A  EGH + V  L  +GA +  N  G S +  AI   +++ V   +  + W+ ++  + H +     I  +I    +    LLD+C+ E+  +K  P++ V+Y F+ +      P S  + +             L +M +++R+ LL HP+  + L +KW  +G     ++  ++ + +I +T  + ++  +L      ++    I   + Q  ++K A   +  SNI+   I  I +II V +L                  C+S   I++S         +W+     +F S+  F+ YLQ   S GI ++MF ++L+T++R++F FF +   F   FY+    Q  F     A M+S  MM G+ +    F+      +  Y   + I + +F +++ I L NL+IGLAVGDI ++   A ++   ++V     +E  +P
Sbjct:  204 EEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLA-TSCSAWKS----VNLLLSKGADVKLKDHYGRNFLHLMVLQPG--GLKNLNSEFLQMENIKKLVSDEDCEGCTPLHYACRHGVPNSVNNLL--GLNMSLYSKSKNKRSPLHFAACYGRFNTCQRLLRFMSDGRLLNDGDEKGMTPLHLAAENGHEKIV-FLLLKRGALLLSDHRGWTALHYAALSGYTRTIKTLL--ETSIGLIDKTDKEQNTAMHLAGREGHAKAVSLLLENGASILLNANGASYIHEAIRFGKKDTVLVSIQSERWEEILQTFSHTSSYKCAILEMIYHLPESFKTLLDRCMTESIGDKKSPDFHVEYNFTYLQC----PLSFRKAHKRENNVHYEPLTTLNAMVQHQRIDLLSHPVCKEYLLMKWMAYGFKAHLLNLAIYSLGLIPLTLMISNDFYVL------IQLLFGIVKEAVQLCQQKLAYIGD-GSNIIDWSIY-ISSIIFVSSL------------------CHS--PIVVSG-------LQWQFGAIAVFASWVNFLIYLQRFESYGIYIVMFWEILRTLLRIVFLFFFLILAFGLSFYVFLYPQVTFSRPYFALMQSFTMMLGDINYEEGFLKPLLDDQIEYPFFTFIHLIMFTLLIPILLMNLLIGLAVGDIAEVQRNACLKRIAMQVSLHTSLEKKLP 941          

HSP 2 Score: 79.337 bits (194), Expect = 2.168e-14
Identity = 79/316 (25.00%), Postives = 143/316 (45.25%), Query Frame = 3
Query:  210 ADGKTIFHTASIINYPILFKLM-KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNI-------FAAND-CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKD----CLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            ++   + H A + N  ++  ++ +S +  IN  D+  N+PLH A+  NKLD +KVLL  GA+ N+ N    +P+ L      A++     I L++    D+N+      T       K+  E +  +        R   +  Q+ +   P+H    +G+L+   ++L+ G            F  N+   PLH+A + G++D+VK  ++   K +  R S+ ++A+H AA  G  EI KF++    D        D      L  +     + + EYL+++  N++  D+   T L  A +    + V  L + GA
Sbjct:   46 SEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHL------AIQLFHNSIALILHSTTDINLEGDLGNTPVMQACCKDNHEALNLLL------GRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKID-LRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLSKGA 348          

HSP 3 Score: 73.9442 bits (180), Expect = 9.472e-13
Identity = 77/314 (24.52%), Postives = 141/314 (44.90%), Query Frame = 3
Query:  306 DNDLNSPLHHAIINNKLDMVKVLLKFGA--NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETN----VNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            D+   +PLHHA     +D++ +++   A   IN  +    TP+  A       KN    +K+L+   AD NI++    +  H L ++     I  I  + ++      IN +  +  +P+  A    N E   +LL  GA         C P+H+ A  G L  + ++L    +          F ++ +SS +H+A +NGN ++ K  I  G     +       L FAA  G   I++++++  ++    V+L D    T LH++    H+++ + L + GA I S++   ++PL +A S +    V   L+
Sbjct:   44 DDSEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQ-----KNKLDCLKVLLCRGADPNIQNCYRLSPLH-LAIQLFHNSIALILHSTTD------INLEGDLGNTPVMQACCKDNHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLS 345          

HSP 4 Score: 72.0182 bits (175), Expect = 3.372e-12
Identity = 70/273 (25.64%), Postives = 122/273 (44.69%), Query Frame = 3
Query:  441 MEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL----SDNMFGQSPLDIAISNNQENVVKFFL---NDDN 1214
            +E+ A  N+  +   ++     L  RD    T  H+      I+++  I    +E    E IN+ D    +PLH+AV    L+  +VLL  GA+PNI       PLH+A +  H + + L+L+S+       D   +  +    ++ N+E    L+G G     +++ G   +   A  G L  ++ +L K        + ++N  +  K + LH A   G++ VV+   + GA +    SDN    + L  A +     +VKF +   +DDN
Sbjct:   18 LELIAEGNVCRVRNFVLKNPGSLAARDDSEATPLHHAAKLGNIDLMLMIVNESAE----EGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFH-NSIALILHSTTDINLEGDLGNTPVMQACCKD-NHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDN---ATALHFAATQGATEIVKFMVSSYSDDN 281          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:NCBI gene;Acc:100158526])

HSP 1 Score: 225.713 bits (574), Expect = 8.719e-61
Identity = 215/796 (27.01%), Postives = 385/796 (48.37%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKS--RDIN--INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIF--AANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNL---HDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH-----------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETG---C----KINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G  + + ++  +N+    L  AVQN N+D++K C+ +   I+ +Q+D  T  H A+      + K M S   D N  +++ D +  +PLH +++ + +++ + L+  GANI+  +K+ +TP+LLA    SA K+    + LL+ + AD+ ++D+  +   H +V++     +K +     + + I +L++ +D    +PLHYA   G       LL  G N +++  + N   PLH AA  G  +  + LL          D +E   + +H+AAENG+ +I  FL+      L  D  G   L +AA SG    I+ LL  ET++ L    D+ + TA+H A  EGH + V  L  +GA +  N  G S +  AI   +++ V   +  + W+ ++  + H +     I  +I    +    LLD+C+ E+  +K  P++ V+Y F+ +      P S  + +             L +M +++R+ LL HP+  + L +KW  +G     ++  ++ + +I +T  ++SN+   D+    V  G   C     I   + Q  ++K A   +  SNI+   I  I +II V +L                  C+S   I++S         +W+     +F S+  F+ YLQ   S GI ++MF ++L+T++R++F FF +   F   FY+    Q  F     A M+S  MM G+ +    F+      +  Y   + I + +F +++ I L NL+IGLAVGDI ++   A ++   ++V     +E  +P
Sbjct:  226 EEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLA-TSCSAWKS----VNLLLSKGADVKLKDHYGRNFLHLMVLQPG--GLKNLNSEFLQMENIKKLVSDEDCEGCTPLHYACRHGVPNSVNNLL--GLNMSLYSKSKNKRSPLHFAACYGRFNTCQRLLRFMSDGRLLNDGDEKGMTPLHLAAENGHEKIV-FLLLKRGALLLSDHRGWTALHYAALSGYTRTIKTLL--ETSIGLIDKTDKEQNTAMHLAGREGHAKAVSLLLENGASILLNANGASYIHEAIRFGKKDTVLVSIQSERWEEILQTFSHTSSYKCAILEMIYHLPESFKTLLDRCMTESIGDKKSPDFHVEYNFTYLQC----PLSFRKAHKRENNVHYEPLTTLNAMVQHQRIDLLSHPVCKEYLLMKWMAYGFKAHLLNLAIYSLGLIPLT-LMISND-FYDTQYTRVCMGLVFCMSLFGIVKEAVQLCQQKLAYIGD-GSNIIDWSIY-ISSIIFVSSL------------------CHS--PIVVSG-------LQWQFGAIAVFASWVNFLIYLQRFESYGIYIVMFWEILRTLLRIVFLFFFLILAFGLSFYVFLYPQVTFSRPYFALMQSFTMMLGDINYEEGFLKPLLDDQIEYPFFTFIHLIMFTLLIPILLMNLLIGLAVGDIAEVQRNACLKRIAMQVSLHTSLEKKLP 974          

HSP 2 Score: 79.337 bits (194), Expect = 2.019e-14
Identity = 79/316 (25.00%), Postives = 143/316 (45.25%), Query Frame = 3
Query:  210 ADGKTIFHTASIINYPILFKLM-KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNI-------FAAND-CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKD----CLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            ++   + H A + N  ++  ++ +S +  IN  D+  N+PLH A+  NKLD +KVLL  GA+ N+ N    +P+ L      A++     I L++    D+N+      T       K+  E +  +        R   +  Q+ +   P+H    +G+L+   ++L+ G            F  N+   PLH+A + G++D+VK  ++   K +  R S+ ++A+H AA  G  EI KF++    D        D      L  +     + + EYL+++  N++  D+   T L  A +    + V  L + GA
Sbjct:   68 SEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHL------AIQLFHNSIALILHSTTDINLEGDLGNTPVMQACCKDNHEALNLLL------GRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKID-LRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLSKGA 370          

HSP 3 Score: 73.9442 bits (180), Expect = 8.736e-13
Identity = 77/314 (24.52%), Postives = 141/314 (44.90%), Query Frame = 3
Query:  306 DNDLNSPLHHAIINNKLDMVKVLLKFGA--NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETN----VNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            D+   +PLHHA     +D++ +++   A   IN  +    TP+  A       KN    +K+L+   AD NI++    +  H L ++     I  I  + ++      IN +  +  +P+  A    N E   +LL  GA         C P+H+ A  G L  + ++L    +          F ++ +SS +H+A +NGN ++ K  I  G     +       L FAA  G   I++++++  ++    V+L D    T LH++    H+++ + L + GA I S++   ++PL +A S +    V   L+
Sbjct:   66 DDSEATPLHHAAKLGNIDLMLMIVNESAEEGINALDSRGNTPLHWAVQ-----KNKLDCLKVLLCRGADPNIQNCYRLSPLH-LAIQLFHNSIALILHSTTD------INLEGDLGNTPVMQACCKDNHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLS 367          

HSP 4 Score: 71.2478 bits (173), Expect = 6.092e-12
Identity = 76/297 (25.59%), Postives = 134/297 (45.12%), Query Frame = 3
Query:  369 VLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL----SDNMFGQSPLDIAISNNQENVVKFFL---NDDN 1214
            V L+ G+   +C+ D   P+L+   ++ A  N+  +   ++     L  RD    T  H+      I+++  I    +E    E IN+ D    +PLH+AV    L+  +VLL  GA+PNI       PLH+A +  H + + L+L+S+       D   +  +    ++ N+E    L+G G     +++ G   +   A  G L  ++ +L K        + ++N  +  K + LH A   G++ VV+   + GA +    SDN    + L  A +     +VKF +   +DDN
Sbjct:   22 VFLQTGS---VCSLD-DNPLLI--FKLIAEGNVCRVRNFVLKNPGSLAARDDSEATPLHHAAKLGNIDLMLMIVNESAE----EGINALDSRGNTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFH-NSIALILHSTTDINLEGDLGNTPVMQACCKD-NHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDN---ATALHFAATQGATEIVKFMVSSYSDDN 303          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Match: trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:NCBI gene;Acc:100158526])

HSP 1 Score: 205.682 bits (522), Expect = 3.382e-54
Identity = 203/792 (25.63%), Postives = 379/792 (47.85%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKS--RDIN--INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVK---------EKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIF--AANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNL---HDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNG--SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYE-VQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCK-INNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCL---YEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G  + + ++  +N+    L  AVQN N+D++K C+ +   I+ +Q+D  T  H A+      + K M S   D N  +++ D +  +PLH +++ + +++ + L+  GANI+  +K+ +TP+LLA    SA K+    + LL+ + AD+ ++D+  +   H +V++         E ++M     ENI      +L++ +D    +PLHYA   G       LL  G N +++  + N   PLH AA  G  +  + LL          D +E   + +H+AAENG+ +I  FL+      L  D  G   L +AA SG    I+ LL  ET++ L    D+ + TA+H A  EGH + V  L  +GA +  N  G S +  AI   +++ V   +  + W+ ++  + H +    + +E +    E   + +L+          ++ N+  +Q   S   A   +     E    L +M +++R+ LL HP+  + L +KW  +G     ++  ++ + +I +T  +++  R    +S+ +   C+ +  +   NS  K      +   +  + + GI  +   + L  QK   + +      ++    ++I +S+    P +    +W+     +F S+  F+ YLQ   S GI ++MF ++L+T++R++F FF +   F   FY+    Q  F     A M+S  MM G+ +    F+      +  Y   + I + +F +++ I L NL+IGLAVGDI ++   A ++   ++V     +E  +P
Sbjct:  151 EEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLA-TSCSAWKS----VNLLLSKGADVKLKDHYGRNFLHLMVLQPGGLKNLNSEFLQM-----ENIK-----KLVSDEDCEGCTPLHYACRHGVPNSVNNLL--GLNMSLYSKSKNKRSPLHFAACYGRFNTCQRLLRFMSDGRLLNDGDEKGMTPLHLAAENGHEKIV-FLLLKRGALLLSDHRGWTALHYAALSGYTRTIKTLL--ETSIGLIDKTDKEQNTAMHLAGREGHAKAVSLLLENGASILLNANGASYIHEAIRFGKKDTVLVSIQSERWEEILQTFSHTSSYKCAILEMIYHLPESFKVGILN----------VEYNFTYLQCPLSFRKAHKRENNVHYEPLTTLNAMVQHQRIDLLSHPVCKEYLLMKWMAYGFKAHLLNLAIYSLGLIPLTLLIINAVR---PESLAIIEECEHLKRTDFYNSSMKQEFCMGL---VFCMSLFGI--VKEAVQLCQQKLAYIGDGSNIIDWSIYISSIIFVSSLCHSPIVVSGLQWQFGAIAVFASWVNFLIYLQRFESYGIYIVMFWEILRTLLRIVFLFFFLILAFGLSFYVFLYPQVTFSRPYFALMQSFTMMLGDINYEEGFLKPLLDDQIEYPFFTFIHLIMFTLLIPILLMNLLIGLAVGDIAEVQRNACLKRIAMQVSLHTSLEKKLP 904          

HSP 2 Score: 72.0182 bits (175), Expect = 3.487e-12
Identity = 72/279 (25.81%), Postives = 125/279 (44.80%), Query Frame = 3
Query:  318 NSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNI-------FAAND-CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKD----CLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            N+PLH A+  NKLD +KVLL  GA+ N+ N    +P+ L      A++     I L++    D+N+      T       K+  E +  +        R   +  Q+ +   P+H    +G+L+   ++L+ G            F  N+   PLH+A + G++D+VK  ++   K +  R S+ ++A+H AA  G  EI KF++    D        D      L  +     + + EYL+++  N++  D+   T L  A +    + V  L + GA
Sbjct:   30 NTPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHL------AIQLFHNSIALILHSTTDINLEGDLGNTPVMQACCKDNHEALNLLL------GRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKID-LRQSDNATALHFAATQGATEIVKFMVSSYSDDNKIVDLPDGNDETPLHKSVLFDHVELAEYLISEGANIDSEDKESRTPLLLATSCSAWKSVNLLLSKGA 295          

HSP 3 Score: 62.7734 bits (151), Expect = 2.421e-9
Identity = 55/203 (27.09%), Postives = 95/203 (46.80%), Query Frame = 3
Query:  651 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL----SDNMFGQSPLDIAISNNQENVVKFFL---NDDN 1214
            +PLH+AV    L+  +VLL  GA+PNI       PLH+A +  H + + L+L+S+       D   +  +    ++ N+E    L+G G     +++ G   +   A  G L  ++ +L K        + ++N  +  K + LH A   G++ VV+   + GA +    SDN    + L  A +     +VKF +   +DDN
Sbjct:   31 TPLHWAVQKNKLDCLKVLLCRGADPNIQNCYRLSPLHLAIQLFH-NSIALILHSTTDINLEGDLGNTPVMQACCKD-NHEALNLLLGRGAKLCKQNKLGCYPIHMTAFMGSLKCMDIILKKGEEMGYTIDHHINFTNNEKSSPLHLAVQNGNIDVVKACISYGAKIDLRQSDN---ATALHFAATQGATEIVKFMVSSYSDDN 228          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:MGI Symbol;Acc:MGI:3522699])

HSP 1 Score: 197.593 bits (501), Expect = 1.216e-51
Identity = 194/790 (24.56%), Postives = 364/790 (46.08%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHA-----------LKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPS--KLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            ++NG + +  ++  ++     L  AVQ+ +LD+IK+CLD    I+  +       H A+      + KLM      S DI +N  D +  + LH A + +  D+ + L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D+  +   H L V++   +     E +      EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L IA+ N ++ VV   + +  W   +  + H++ S+   I  +++   +    LLD C+I +T +K   +Y ++Y F  +      P S++++              L  M ++ R++LL HP+  + L +KW  +G     ++   + + +I +T  V+  +  +  +S  +  G     SST      + ++  +   ++++ +  I      +  I Q+ RN F  + N          II   P    +P   +W+     IF  +  F+ YLQ   + GI ++M + + KT++R    F  +   F   FY++   QD F    L+ +++ +MM G+ +    F+      +  Y V +   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P
Sbjct:  223 EKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDI-VNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASW-----NIVNLLLCKGAKVDIKDHLGRNFLH-LTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQC----PLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIING----TSSTHEERIDTLNSFPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLP 994          

HSP 2 Score: 106.301 bits (264), Expect = 7.421e-23
Identity = 92/343 (26.82%), Postives = 169/343 (49.27%), Query Frame = 3
Query:   69 ENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD--FCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYE--NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR---DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            E+ I  +  L + +++  C L +A     ++++++ ++   C  +N     G T  H A+  N     K + S+  N N+ + ++ SPLH A+     +++KVL +  A NINL  ++  T ++  C      K+ S  +++L+ + A L       DY +     +   K+ +E+I    E    S    I  +N +     SPLH AV  G+L++ ++ L+ GA+ ++     C+ LH AA +G  DIVKL+++S   S       D N+ + +H A+   ++++ ++LI +G D  + D  GR+ L+ A  S    I+  LL K   V++ D +    LH
Sbjct:   48 EDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCA-----KDNSEALQILLEKGAKLCKSNKWGDYPVHQAA-FSGAKKCMELILAYGEKNGYSRETHINFVNHKK---ASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLH 381          

HSP 3 Score: 72.0182 bits (175), Expect = 3.139e-12
Identity = 65/264 (24.62%), Postives = 115/264 (43.56%), Query Frame = 3
Query:  540 FHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL--------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS--PLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLE 1301
             H+   + ++E+++ I    S     E++N  D    +PLH A     +E  + LL  GANPN+   N   PLHIA    + +++K+L      +      N ++A+       N E  + L+  G      +++G   +  AA SG    +E +L        ++ET++N  +  K + LH A   G L +++    +GA + D M       L  A +    ++VK  ++        +Y    GSS I N +  N++  L 
Sbjct:   68 LHHAAAEGQVELMELIINGSS----CEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHI-DMMENAKCMALHFAATQGATDIVKLMIS--------SY---TGSSDIVNAVDGNQETLLH 315          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Trpa1 (transient receptor potential cation channel, subfamily A, member 1 [Source:MGI Symbol;Acc:MGI:3522699])

HSP 1 Score: 190.274 bits (482), Expect = 3.264e-49
Identity = 190/790 (24.05%), Postives = 359/790 (45.44%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHA-----------LKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPN--VTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            ++NG + +  ++  ++     L  AVQ+ +LD+IK+CLD    I+  +       H A+      + KLM      S DI +N  D +  + LH A + +  D+ + L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D+  +   H L V++   +     E +      EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L IA+ N ++ VV   + +  W   +  + H++ S+   I  +++   +    LLD C+I +T +K   +Y ++Y F  +      P S++++              L  M ++ R++LL HP+  + L +KW  +G     ++   + + +I +T  V+  +  +  +S  +     IN +S+ + E+      N  +  N  +  ++   +II V+ L +                              +P   +W+     IF  +  F+ YLQ   + GI ++M + + KT++R    F  +   F   FY++   QD F    L+ +++ +MM G+ +    F+      +  Y V +   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P
Sbjct:  223 EKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDI-VNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASW-----NIVNLLLCKGAKVDIKDHLGRNFLH-LTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQC----PLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGI-----INGTSSTHEERIDTLKRNYFLDYNNALEWVIYTTSIIFVLPLFLN-----------------------------IPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLP 964          

HSP 2 Score: 106.301 bits (264), Expect = 7.349e-23
Identity = 92/343 (26.82%), Postives = 169/343 (49.27%), Query Frame = 3
Query:   69 ENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD--FCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYE--NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR---DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            E+ I  +  L + +++  C L +A     ++++++ ++   C  +N     G T  H A+  N     K + S+  N N+ + ++ SPLH A+     +++KVL +  A NINL  ++  T ++  C      K+ S  +++L+ + A L       DY +     +   K+ +E+I    E    S    I  +N +     SPLH AV  G+L++ ++ L+ GA+ ++     C+ LH AA +G  DIVKL+++S   S       D N+ + +H A+   ++++ ++LI +G D  + D  GR+ L+ A  S    I+  LL K   V++ D +    LH
Sbjct:   48 EDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCA-----KDNSEALQILLEKGAKLCKSNKWGDYPVHQAA-FSGAKKCMELILAYGEKNGYSRETHINFVNHKK---ASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLH 381          

HSP 3 Score: 72.0182 bits (175), Expect = 2.783e-12
Identity = 65/264 (24.62%), Postives = 115/264 (43.56%), Query Frame = 3
Query:  540 FHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL--------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS--PLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLE 1301
             H+   + ++E+++ I    S     E++N  D    +PLH A     +E  + LL  GANPN+   N   PLHIA    + +++K+L      +      N ++A+       N E  + L+  G      +++G   +  AA SG    +E +L        ++ET++N  +  K + LH A   G L +++    +GA + D M       L  A +    ++VK  ++        +Y    GSS I N +  N++  L 
Sbjct:   68 LHHAAAEGQVELMELIINGSS----CEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHI-DMMENAKCMALHFAATQGATDIVKLMIS--------SY---TGSSDIVNAVDGNQETLLH 315          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 125.176 bits (313), Expect = 1.164e-28
Identity = 104/349 (29.80%), Postives = 160/349 (45.85%), Query Frame = 3
Query:  216 GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDK---SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLL--NSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFLN---DDNWQNVMAY 1235
            G T  H AS + +  + K +  R  + NV +  + +PLH A      ++ K LL+  A  N   KD QTP  L C   +A    +G++KLL+   A  N+      T  H    +  ++    + E  +         SQ  + K   +PLH A   G + + ++LLE  A+PN    N   PLH+A    +LDIVKLLL    S  S  +   N  + +HIAA+    E+ + L+  G    A+   G   L  AA+ G   ++  LL+K+ N NL ++  +T LH  + EGH+ V   L   G  + +    G +PL +A       +VKF L    D N +  + Y
Sbjct:    1 GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTP--LHC---AARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEA---------SQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAW---NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGY 332          

HSP 2 Score: 106.301 bits (264), Expect = 6.934e-23
Identity = 87/349 (24.93%), Postives = 158/349 (45.27%), Query Frame = 3
Query:  120 QCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIA 1163
            +  L  A +  + ++ K  L      N K  D +T  H A+ I +  + KL+     + N+     ++PLH A     +D    LL+  A+     K   TP     + ++A      + +LL+   A  N    +  T  H  V    ++++K +        R    +S      +PLH A     +E+ + LL+ G + N  +     PLH+AA++GH ++V LLL S + +    + +  + +H+ ++ G+  +   LI  G    A    G   L  A+  G + ++++LL  + +VN   ++  + LHQAA +GH  +V  L  +GA  ++ +  G +PL IA
Sbjct:   35 ETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTP-----LHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLP------RGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLL-SKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIA 371          

HSP 3 Score: 87.8113 bits (216), Expect = 4.053e-17
Identity = 78/330 (23.64%), Postives = 144/330 (43.64%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL 1103
            D Q  L  A +  +  ++K+ L+     N     G T  HTA+   +      +  ++ +         +PLH A    K+ + ++LL+  A+ N   K+  TP+ +A        NL  I+KLL+      +   ++  T  H    + +IE+ + + +    +      N++ +   +PLH A   G+ E+  +LL   AN N+   +   PLH+ +++GH+ +  +L+      +        + +H+A+  GN ++ KFL+    D  AK + G + L  AA+ G   I+  LL    + N       T L  A   G++ V   L
Sbjct:   66 DDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHH----NNLD-IVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSA------NAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMG-YTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVL 383          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ankrd50 (ankyrin repeat domain 50 [Source:MGI Symbol;Acc:MGI:2139777])

HSP 1 Score: 124.405 bits (311), Expect = 2.228e-28
Identity = 91/342 (26.61%), Postives = 168/342 (49.12%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASII-----NYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYE-NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL 1103
            +++ DN+G+  L  A    + +I++  LD   ++N +  DG+T    A++       +  +  L+  R   ++  D D  +PL  A     +D+V +LL+ GA+++  + + +TP+L A     A    + ++  L+   A ++  D + +T+      +  +E+++ + +  + ES R       D    +PLH A   G+  IC+ L+E GA  N    +  +P  +A+++GH D V++LL +    +  R  +  +A+ +AA  G+ +I + L   G D   KD  GR TL   A    L + EY L    NV   D    TALH +  +GH+++V+ L
Sbjct:  633 VNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAA-----ASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDESHR-------DDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNID-QRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHVEMVRVL 961          

HSP 2 Score: 106.686 bits (265), Expect = 7.106e-23
Identity = 78/306 (25.49%), Postives = 151/306 (49.35%), Query Frame = 3
Query:   93 ALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSR--KSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYL 1004
            A+D  D++G+  LS A    N+++++  LD  +D + +   G T  H A+   + ++ + +  +    N  DND   P   A      D V++LL+  +NI+    D +       + ++AL+    I++LL    AD+N +D D +   + L ++ ++ M ++  EN +       + + D   ++ LH +   G++E+ +VL+   A+ N         L  AA +GH+ +V+LL+         C   +  ++A+ IAA+ G+ ++ + L+  G D    D+FGR  +  AA++G   II+ L
Sbjct:  769 AVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDESHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRN-----ALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGAN------VEASDAEGRTALHVSCWQGHVEMVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTC---NQGATALCIAAQEGHVDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 1060          

HSP 3 Score: 86.6557 bits (213), Expect = 9.160e-17
Identity = 90/411 (21.90%), Postives = 173/411 (42.09%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLA----------------------------CMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYA-----VSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLND 1208
            I     ++ +D+   C +  A++ +  D I+  LD    +N   ++G+T+   A+      +  L+ SR  ++ + D   ++PL  A       +V  L+  GANIN  ++D  T +  A                             +  +A      I+  L+   A++N  D + +T           E+++ + ++ +E      +N +D+  ++ L  A      S G+  +  +L++ GA  +    +   PL +AA +GH+D+V LLL      +   D+N  + +  AA  G+  +   L+  G    + D  GR  L  A+  G + ++  LL +  + +  D    T LH AA EGH  + + L   GA  ++ +  G+ P  +A      + V+  L +
Sbjct:  464 IRAGAHVNSEDDHTSCIVRQALERE--DSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAE------VNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVD-HTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDESHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN 865          

HSP 4 Score: 83.5741 bits (205), Expect = 9.443e-16
Identity = 61/253 (24.11%), Postives = 123/253 (48.62%), Query Frame = 3
Query:   45 F*SSKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
            F   +LI E  I      +E DNDG+     A Q  + D ++I L+   +I+ +  DG+     A++  +  + +L+ S   ++N  D D    L+   + N+L M +  L+ GAN+   + + +T + ++C +         ++++LI   AD+N  D + ++       +  +++++ + E+ +      +++       + L  A   G++++ QVLLE GA+PN         + +AA+ GH  I+KLL
Sbjct:  819 FEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHVE-----MVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGA------VVDHTCNQGATALCIAAQEGHVDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLL 1060          

HSP 5 Score: 78.1814 bits (191), Expect = 3.730e-14
Identity = 78/294 (26.53%), Postives = 138/294 (46.94%), Query Frame = 3
Query:  339 IINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHD-----QMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFL 1202
            +I  + +++++L++ GA++N  ++D  T    +C+   AL+    I + L+   A +N  D + +T+         ++++  +   +S    +E+   +D    +PL  A   G+ ++   L+  GAN N    +    L  AA  GH ++V  LL +  K +C  D++  +A+  AA  G+ +I   L+  G +    D  GR  L+ AA  G   I+E+LL     VN  D      + V AL   A++GH  VV  L   GA +   +  G +PL +A      +VV   L
Sbjct:  452 LIPKEQEVLQLLIRAGAHVN--SEDDHT----SCIVRQALEREDSI-RTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLL---VSRGADLEI---EDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCA-DADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLL 731          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 122.479 bits (306), Expect = 8.058e-28
Identity = 101/362 (27.90%), Postives = 172/362 (47.51%), Query Frame = 3
Query:  120 QCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSA-----IHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARS--GCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQE 1181
            Q  L  A+ N + D ++  +    D+N +  + +T  H A+ +    + +L+      +N  D+   +PLH A+ +   + V++LLK  A++N  +K++QTP+ +A     A+K    ++ LL    +++N+ D   +T  H+       EM+K +        R   IN+ D  D+  +H+A  +G++E+ ++L+  GA           PLH AA  G + +VK LL      +   D NE +A     +H+A  NG   +   LI  G +   K+E G   L FAA S  G L  +E L+    +VN+  +   T LH  A  G     Q +  SGA++  ++  G +PL IA     E
Sbjct:    9 QPSLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAAN-KAVKCAESLVPLL----SNVNVSDRAGRTALHHAAFSGHGEMVKLLL------SRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLL------DLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGAL-CLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTIIQSGAVIDCEDKNGNTPLHIAARYGHE 352          

HSP 2 Score: 107.071 bits (266), Expect = 4.270e-23
Identity = 102/388 (26.29%), Postives = 175/388 (45.10%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYE---NISESQ-------RIELINSQDIV-----------------DKSPLHYAVSIGNLEIC-QVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISN 1172
            ++ KD+     L  AV + + + ++I L    D+N +  + +T  H A+        + +     N+NV D    + LHHA  +   +MVK+LL  GANIN  +K  +  I  A     A      ++KLL+   A++  +D    T  H       I ++K++ +   +++E          +   N QD+V                   +PLH+A +  +  +C ++L+  GA+ N+ + +   PLH+ A  G     + ++ S    +C  D N ++ +HIAA  G+  +   LI  G D   +   G   L  AA SG       LL+   +++  D    T LH AAA G+L+ +  L  +GA  +  + FG+SPL  A +N
Sbjct:   67 VNAKDSKWLTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWA-----AYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTIIQSGAVIDC-EDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAAN 448          

HSP 3 Score: 105.145 bits (261), Expect = 1.481e-22
Identity = 108/441 (24.49%), Postives = 178/441 (40.36%), Query Frame = 3
Query:  108 DNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISAL--------------KNLSGI---------------IKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA----------------------RSGCLPI------------IEYLLTKETNVNLHDQMKVTALHQAAAEGH---LQVVQC-------LWTSGAIL---SDNMFGQSPLDIAISNNQENVVKFFL 1202
            D  G+  L +A  + + +++K+ L    +IN      +   H A+ + +  + KL+ S    +   D    +PLH A  +  + +VK LL  G ++N  N    TP+ +AC     +              KN  G                ++LL+   AD+N++  D KT  H   +  +    + I       Q   +I+ +D    +PLH A   G+  +   L+ +GA+      +   PLH+AA  G  D  + LL+S    +   D    + +H AA  GN E    L+  G D   KD+FGR+ L +AA                        GC P+            +EYLL  + N  + D+    A+H +AA GH   LQ++         + TSG  +   SDN    SPL +A  +     ++  +
Sbjct:  137 DRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTII------QSGAVIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDTPDDFGR-TCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAATSDTDGKCLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLQLIASETPLDVLMETSGTDMLSDSDNRATISPLHLAAYHGHHQALEVLV 570          

HSP 4 Score: 95.9005 bits (237), Expect = 1.183e-19
Identity = 95/360 (26.39%), Postives = 163/360 (45.28%), Query Frame = 3
Query:  159 DIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYEN-----ISESQRIELINSQD-IVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSN-ESSAIHIAAENGNYEIFKFLIGIGKDCLA---KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA--ILSDNMFGQSPLDIAISNNQENVVKFFL 1202
            D  +  L    DI+T    G+T  H A+         L+ +   + N  D    SPLH+A  N     +  L+  GA++N  ++   TP+  A    +  K     ++ L+   A+  IRD       HY         ++ I        + E+   ++++  D     SPLH A   G+ +  +VL+++  + ++  ++   PL +AA KGH++ V +L+N    S   +D   + + IH AA NG+ E  + LIG  +   A   +D  G+  LM +  +G    +  LL K  NV+  D+   TALH+ A  GH + V  L   GA  +L D+  G++P+ ++ +     V+   L
Sbjct:  386 DCCRKLLSSGFDIDTPDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAANCNYQCLFALVGSGASVNDLDERGCTPLHYAATSDTDGK----CLEYLLRNDANPGIRDKQGYNAVHYSAAYGHRLCLQLIASETPLDVLMETSGTDMLSDSDNRATISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQG-ASILVKDYVLKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSR-GRTPIHLSAACGHIGVLGALL 739          

HSP 5 Score: 92.8189 bits (229), Expect = 1.103e-18
Identity = 67/226 (29.65%), Postives = 116/226 (51.33%), Query Frame = 3
Query:  546 YLVVKEKIEMIKWIYENISESQRI-----ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            +L ++++  +++ I+    +  R      E +N QD   ++PLH A  +G+ EI ++L+ +GA  N   +    PLH A      + V++LL  S      RD N  + +HIAA N   +  + L+ +  +    D  GR  L  AA SG   +++ LL++  N+N  D+    A+H AA  GH++VV+ L + GA +   +    +PL  A S+   +VVK+ L+
Sbjct:    3 FLKLRDQPSLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAYLGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEEAVQILLKHSADVNA-RDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLD 227          

HSP 6 Score: 73.1738 bits (178), Expect = 1.210e-12
Identity = 73/321 (22.74%), Postives = 144/321 (44.86%), Query Frame = 3
Query:   87 KNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFG----ANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI--GIGKDCL--AKDEFGRNTLMFAARSGCLPIIEYLLTKETNV 1025
            +NA+D +D +GQ  L  +V N + D +   L+   +++ K   G+T  H  ++  +      +        + D+   +P+H +     + ++  LL+      AN  + +    T +  AC       N       L++E+      D +  +  H  V+ +     + + +++  S    ++N+ D   ++PLH A    ++E  Q+LL   A  N   +    PL +AAE G  + V++L++S+      +D ++++A+H+A   G +E    LI   I    L  A +   +  L  AAR+G   +++ LL K  +V
Sbjct:  643 QNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACY------NGHETCVELLLEQDVFQKIDGNAFSPLHCAVINDNEGAAEMLIDSLGAS----IVNATDSKGRTPLHAAAFTDHVECLQLLLSQNAQVNSADSTGKTPLMMAAENGQTNTVEMLVSSASADLTLQDKSKNTALHLACGKG-HETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLGKGASV 952          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q7Z020|TRPA1_DROME (Transient receptor potential cation channel subfamily A member 1 OS=Drosophila melanogaster OX=7227 GN=TrpA1 PE=2 SV=4)

HSP 1 Score: 253.832 bits (647), Expect = 2.868e-69
Identity = 225/858 (26.22%), Postives = 408/858 (47.55%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEK--IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV-NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNN-QENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYH------------ALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYF---VLSNERILDSDSVDVETGCKIN-NSSTQNSEKKSASAP-----------NVTSNILIIMIVGIRNIIL-----VMTLIVQKFRNLFEFWTNCYFACNSLALIIIS---NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQ---DQDP----FKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYY-------VTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKR-----FIVSWLFQNSF 2444
            ++ G   +  +   D++G   L  AV   ++  +++CL     I+T+Q D  T  H A       + KLM       + + ++  D    +PLH A + +  D+V  L+  GA+IN  +K+ ++P+LLA     A ++    + LLI   A ++++D   + + H++++      +  + +    +++Q   L+N +D +  SPLHYA   G++   + L+  GA  N+   N+  PLH AA  G  + V+ LL+S + S    +S+ +  + +HI+++ G+  + + L+  G   L +D  GRN L  AA SG    IE L +  +++ +  D+   TALH A  E     +  L + G  L  N+   S +D AI     E  +    +++    VMA R D        LI S   +   + DKCI +    K   ++ ++Y F+ +   P   A I E+              AL +M  + R++LL HPLS K L++KW+ +G  F   + L++ ++++ +T +   +++N  +   D+  +   C +     T N  +  + A            N T+ IL   +V +  I+L     ++ +  QK   + E      +     AL++++    P        +      +FLS+F  + +LQ    VGI ++MF ++L+T+I+VL  F  +   F   FYI+     D  P    F N+ ++ +++ +MM GE D V  +V+    YY       +TS +++S+F+++M I L NL+IGLAVGDI  +   A ++   ++V    E+E  +P V  + +   E+ + P + K +     FI+   F N F
Sbjct:  249 EQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLA-----ASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGA-LLHRDHTGRNPLQLAAMSGYTETIELLHSVHSHLLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQC-PFMFAKIDEKTGESITTASPIPLPALNTMVTHGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVTIYSSLMMNNIELKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVTPAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQVGIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQPNHLSFSNIPMSLLRTFSMMLGELDFVGTYVN---TYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQLKRLAMQVVLHTELERKLPHVWLQRVDKMELIEYPNETKCKLGFCDFILRKWFSNPF 1096          

HSP 2 Score: 85.5001 bits (210), Expect = 1.336e-15
Identity = 105/445 (23.60%), Postives = 184/445 (41.35%), Query Frame = 3
Query:  138 AVQNKNLDIIKICLDFCVD---INTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS--GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI---------------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL--LNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAA-RSG------------CLPI----------------------IEYLLTKETNVNL------HDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDD 1211
            A ++ NLD  K    F  D   I  K A G+T  H A+  N   + + ++ ++ + N  DN  N+PLH A+ ++  D +  LL    +  + N+  Q P+ LA  E++ +K+L   G  + +I    D+       +T  H   + +  E  + +               Y  I E+                      R E+I+  D     PLH AV  G+++  ++ L++GA  +    +   P+H+A  +G +DIVKL+  +    K  C    D  + + +H A+   + +I  +L+  G D  A D+  R+ L+ AA RSG            C+ +                       E +   +T   L       D M  + LH A+ +GH++ ++ L   GA ++  N   +SPL  A    + N V+  L+ +
Sbjct:   63 AAESGNLDDFKRL--FMADNSRIALKDAKGRTAAHQAAARNRVNILRYIRDQNGDFNAKDNAGNTPLHIAVESDAYDALDYLLSIPVDTGVLNEKKQAPVHLAT-ELNKVKSLRVMGQYRNVI----DIQQGGEHGRTALHLAAIYDHEECARILITEFDACPRKPCNNGYYPIHEAAKNASSKTMEVFFQWGEQRGCTREEMISFYDSEGNVPLHSAVHGGDIKAVELCLKSGAKISTQQHDLSTPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEGADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSE 500          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q18297|TRPA1_CAEEL (Transient receptor potential cation channel subfamily A member 1 homolog OS=Caenorhabditis elegans OX=6239 GN=trpa-1 PE=2 SV=5)

HSP 1 Score: 216.468 bits (550), Expect = 7.488e-57
Identity = 216/869 (24.86%), Postives = 376/869 (43.27%), Query Frame = 3
Query:   60 LIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADG-KTIFHTASIINYPILFKLMKSRDININVGDNDLNS----PLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKT----------------IFHYLVVKEK---IEMIKWIYEN----ISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG-AILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAY-------RHDNG-----SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLID---------------------AGPDDPASISE-------------RYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILIT---------YFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFACNSLALIII------SNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKN---------YYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQR 2360
            ++  + +    AL+  +N+ +  L  AV+  + + +K  L      + K  D  K + H A+   +  + K +    +      N+LN     PLH A   N+L++V  L++    NI++ ++   TP+++A    S        ++ LI +KA+L I D D +T                I  +L  K K      +K    N    +SE  R  ++N  D    +P+H   S G LE+ Q+L + GA+      ++   LH AA  G    V+ LL    +    +D   +SA+H+AA +G+    K L+  G D  AK+ + +  L  A  SG L   + L+ K   +      K T LH AA  G+  +V+     G  I   +  G++  DIA  N+ ++V + FL  D W+N+M         +H N       +    L+    +L   ++D CI E +  + D    V Y F  +D                     +  D+   + +             +YH LK M + ++L LL HPLS  LLK KW++ G   +     ++LV+I+ +T         Y V + E   DS+  D    C   N++           P+V   I+I  +   + ++    L  +KF  L  +E W +C+    S ALI +      S  S +   ++W      IF  +   ++ ++ +P  GI ++MF  ++KT  R    F      F+  FY+I Q++  F  + ++ +K+  MM GEF+    F   +           +   +  +   F +IM+I L NL++GLAV DI  +  +A ++   ++V  ++++E ++   IQR
Sbjct:  259 MMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKAL----VEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDS-----KKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLLEWDIRLLLMKDEMGNSALHLAARSGHDATTKVLLDNGADKEAKNSYQKTPLQVAVDSGKLETCQRLVAKGAQIESSSDTK-TVLHTAAFYGNESIVRYFIAEGVTIDRRDEEGKTAFDIACENDHKDVARAFLETDQWKNLMIPCDVIPLDKHRNPVNMKRRTPFRTLLTKFPELASFVMDNCI-EKSKEETDSTQSVAYNFEFLDDTYMMRCVSDDGTGEQLIGCKSAYDEDFKLEKDAQSYASNYDRVYKYHPLKLMADAEKLHLLNHPLSKALLKYKWNRLGRPMYYFALFMYLVFIVSLTQYVRHTKAPYNVWNEESYYDSEYFDENETCPQINTTK----------PDVVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIY--STALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPRFGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPEFSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASLHFFIQR 1104          

HSP 2 Score: 103.605 bits (257), Expect = 4.097e-21
Identity = 115/508 (22.64%), Postives = 212/508 (41.73%), Query Frame = 3
Query:   93 ALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTAS--IINYPIL--------------FKLMKSRDININVGDN----DLN----SPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVK------------EKIEMIKWI-------------------YENISESQRIELINSQDIVDKSP--LHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL-----------------------------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLNDDNWQ-NVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPN 1352
            A++ +D D    L YA +  N D +K+ L      NTK  +G T  H AS  I  Y  +              +     + IN  V +N     +N    +PLH+A + +    +  L+K  A+++  + +  TP+LLAC+  S       II+ LI   +++  RD  + T+FH + ++            + +E IK +                    E + +  ++E  NS   +D+    +H+A   G LE+ + L+EAG N N       +PLH+AA+   L++V  L+   + +    D    + + +A  + + +  ++LI    +    D+  R  +   A+   L  +EY+L                              + T VN+ D+ + T +H  A+ G+L+++Q L   GA ++  N   ++ L  A    Q   V+  L    W   ++  + + G+S +    +S  D T     K +++N ++K   N
Sbjct:   76 AVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKADVDAEDDNKMTPLLLACVHGS-----QEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILDHLRKKNKETERSALKSPTRNTLRIVSEDVRRTMVNMVDRDQNTPMHIVASNGYLEMMQLLQKHGASITQVNEDEETALHRAAIGGQTGAVRQLL---EWDIRLLLMKDEMGNSALHLAARSGHDATT----KVLLDNGADKEAKN 571          

HSP 3 Score: 75.8702 bits (185), Expect = 1.246e-12
Identity = 91/399 (22.81%), Postives = 162/399 (40.60%), Query Frame = 3
Query:  189 VDINTKQADGKTIFH-TASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNL-------------------SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGAN--------------------P------------------NIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKET-NVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            +D  T+  + ++I H +A   N   L + +    + +N  D D  +PLH+A      D VK+LL   A  N  N++  TP+ +A   I    ++                     II  L+ E A+++  +    T  HY  +K     +  + +  ++      ++++D    +PL  A   G+ EI Q L++A +N                    P                  N+F      PL +A E  H + +K +L   +K+ C     E   IH AAE G  E+ K L+  G +    +E     L  AA+   L ++ YL+ +E  N+++ D+  +T L  A      + V+ L    A L+  +   ++P+ I    N  + V++ L+
Sbjct:   41 LDARTEADNLRSIIHQSAREGNVNALQEALLKAPLAVNAQDGDFMTPLHYAARYGNYDAVKLLLSKNALPNTKNREGDTPLHIASKYIYGYSDICSIIDEDQADSARKYNTATKKIINALVSENAEIDPVNKYQLTPLHYAAMKSNFSALHALIKLKAD------VDAEDDNKMTPLLLACVHGSQEIIQELIKANSNVTKRDQRLNTVFHIVALRGEPEYLEMMMDHDPVEAIKALNLFNNEKKTPLRMAVEGNHPETLKKILQMEKKNSCKWMDREKELIHFAAEKGFLEVLKALVEAGGNKNELNEVKAVPLHVAAQMNQLEVVSYLIEEEKDNIDVVDEQGLTPLMMAVTHDSKKCVEYLIAKKANLTITDKDERTPVFIGAKFNALSSVEYILD 433          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|O75762|TRPA1_HUMAN (Transient receptor potential cation channel subfamily A member 1 OS=Homo sapiens OX=9606 GN=TRPA1 PE=1 SV=3)

HSP 1 Score: 200.29 bits (508), Expect = 1.205e-51
Identity = 202/824 (24.51%), Postives = 379/824 (46.00%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----DPAS--ISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSFIFTKSE 2462
            +E+G + +  ++  +N     L  AVQN +L++IK+CLD    I+  +    T  H A+      + KLM      S DI +N  D    + LH A + +  ++   L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D   +   H L V++   +     E +   Q  EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L +A+ N ++ VV   +    W   +  + H++  +   I  +I+   +    LLD C++ +T +K   +Y ++Y F  +    +     P    I E   AL +M +  R++LL HP+  + L +KW  +G     ++   + + +I +T  V++ +  +  +S  +     IN +S  +    + ++  + + ++++ +  I         I Q+ RN F   +N          II   P   ++P   +W+     ++  +  F+ YLQ   + GI ++M + +LKT++R    F  +   F   FYI+   QDPF +  L+ +++ +MM G+ +    F+      +  + V S   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P    R +        P + +   ++  +F   F+F   E
Sbjct:  222 EEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDI-VNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASW-----NIVNLLLSKGAQVDIKDNFGRNFLH-LTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGI-----INETSDHSEILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIF--CFLFCTGE 1028          

HSP 2 Score: 88.9669 bits (219), Expect = 1.063e-16
Identity = 90/336 (26.79%), Postives = 157/336 (46.73%), Query Frame = 3
Query:   81 NVKNALDEKDNDGQCCLSYAVQNKNLDII-KICLDFCVDINTKQAD-GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVV---KEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECF---RDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            N +  L   D+     L YA     ++++ KI  D  +++  +  D G T  H A   N     K + SR  N N+ + ++ +PLH A+     +++KVLL+    ++NL  ++  T +++AC       N S  +++L+ + A     +       H       KE +E+I    E    S+++  IN  +    +PLH AV  G+LE+ ++ L+ GA  +      C  +H AA +G  +IVKL+++S   S       D    + +H A+   ++E+  +LI +G D    D  GR+ L+ A  S    I+  LL+K   V++ D      LH
Sbjct:   51 NKQKKLKRCDDMDTFFLHYAAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACT-----TNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLH-INFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRNFLH 380          

HSP 3 Score: 85.8853 bits (211), Expect = 1.093e-15
Identity = 65/237 (27.43%), Postives = 114/237 (48.10%), Query Frame = 3
Query:  525 DMKTIF-HYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSG---CLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ-SPLDIAISNNQENVVKFFLN 1205
            DM T F HY   + +IE++    E I+    +E+++  D    +PLH AV    +E  + LL  GANPN+   N   PLHIA +  + +++K+LL            N ++A+ IA    N E  + L+  G      +++G   +  AA SG   C+ II     E+  +++ ++N  +  K T LH A   G L++++    +GA +     G+ + +  A +     +VK  ++
Sbjct:   61 DMDTFFLHYAAAEGQIELM----EKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMIS 293          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q8BLA8|TRPA1_MOUSE (Transient receptor potential cation channel subfamily A member 1 OS=Mus musculus OX=10090 GN=Trpa1 PE=1 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 8.503e-51
Identity = 194/790 (24.56%), Postives = 364/790 (46.08%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHA-----------LKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPS--KLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            ++NG + +  ++  ++     L  AVQ+ +LD+IK+CLD    I+  +       H A+      + KLM      S DI +N  D +  + LH A + +  D+ + L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D+  +   H L V++   +     E +      EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L IA+ N ++ VV   + +  W   +  + H++ S+   I  +++   +    LLD C+I +T +K   +Y ++Y F  +      P S++++              L  M ++ R++LL HP+  + L +KW  +G     ++   + + +I +T  V+  +  +  +S  +  G     SST      + ++  +   ++++ +  I      +  I Q+ RN F  + N          II   P    +P   +W+     IF  +  F+ YLQ   + GI ++M + + KT++R    F  +   F   FY++   QD F    L+ +++ +MM G+ +    F+      +  Y V +   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P
Sbjct:  223 EKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDI-VNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASW-----NIVNLLLCKGAKVDIKDHLGRNFLH-LTVQQPYGLRNLRPEFMQMQHIKELVMDEDNDGCTPLHYACRQGVPVSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRNKRWDECLQVFTHNSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQC----PLSMTKKVAPTQDVVYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGIING----TSSTHEERIDTLNSFPIKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALEWVIYTTSIIFVLPLFLNIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLP 994          

HSP 2 Score: 106.301 bits (264), Expect = 5.191e-22
Identity = 92/343 (26.82%), Postives = 169/343 (49.27%), Query Frame = 3
Query:   69 ENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD--FCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYE--NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR---DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            E+ I  +  L + +++  C L +A     ++++++ ++   C  +N     G T  H A+  N     K + S+  N N+ + ++ SPLH A+     +++KVL +  A NINL  ++  T ++  C      K+ S  +++L+ + A L       DY +     +   K+ +E+I    E    S    I  +N +     SPLH AV  G+L++ ++ L+ GA+ ++     C+ LH AA +G  DIVKL+++S   S       D N+ + +H A+   ++++ ++LI +G D  + D  GR+ L+ A  S    I+  LL K   V++ D +    LH
Sbjct:   48 EDFIKNRRKLSKYEDENLCPLHHAAAEGQVELMELIINGSSCEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCA-----KDNSEALQILLEKGAKLCKSNKWGDYPVHQAA-FSGAKKCMELILAYGEKNGYSRETHINFVNHKK---ASPLHLAVQSGDLDMIKMCLDNGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLAEYLISVGADINSTDSEGRSPLILATASASWNIVNLLLCKGAKVDIKDHLGRNFLH 381          

HSP 3 Score: 72.0182 bits (175), Expect = 2.196e-11
Identity = 65/264 (24.62%), Postives = 115/264 (43.56%), Query Frame = 3
Query:  540 FHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL--------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS--PLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLE 1301
             H+   + ++E+++ I    S     E++N  D    +PLH A     +E  + LL  GANPN+   N   PLHIA    + +++K+L      +      N ++A+       N E  + L+  G      +++G   +  AA SG    +E +L        ++ET++N  +  K + LH A   G L +++    +GA + D M       L  A +    ++VK  ++        +Y    GSS I N +  N++  L 
Sbjct:   68 LHHAAAEGQVELMELIINGSS----CEVLNIMDGYGNTPLHCAAEKNQVESVKFLLSQGANPNLRNRNMMSPLHIAVHGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKKCMELILAYGEKNGYSRETHINFVNHKKASPLHLAVQSGDLDMIKMCLDNGAHI-DMMENAKCMALHFAATQGATDIVKLMIS--------SY---TGSSDIVNAVDGNQETLLH 315          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Match: sp|Q6RI86|TRPA1_RAT (Transient receptor potential cation channel subfamily A member 1 OS=Rattus norvegicus OX=10116 GN=Trpa1 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 6.994e-50
Identity = 189/770 (24.55%), Postives = 357/770 (46.36%), Query Frame = 3
Query:  129 LSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR--DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVM-AYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHA-----------LKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEK-KSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNC--YFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVS----GKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            L  AVQ+ +LD+IK+CLD    I+  +       H A+      + KLM      S DI +N  D +  + LH A + +  D+   L+  GA+IN  + + ++P++LA    S       I+ LL+ + A ++I+D+  +   H L V++   +     E +      EL+  +D    +PLHYA   G       LL    + +  + +   PLH AA  G ++  + LL     +      D +  + +H+AA+NG+ ++ + L+  G   L+ D  G   L  A+  G    ++ +L  +TN+   D++     TALH AA EGH + V  L +  A +  N    S L IA+ N ++ VV   +    W   +  + HD+ S+   I  +++   +    LLD C+I +T +K   +Y ++Y F  +      P S++++              L  M ++ R++LL HP+  + L +KW  +G     ++   + + +I +T  V+  +  +  +S  +     IN + + + E+  + ++  +   ++++ +  I      +  I Q+ RN F  + N   +    +  + ++     +P   +W+     IF  +  F+ YLQ   + GI ++M + + KT++R    F  +   F   FY++   QD F    L+ +++ +MM G+ +    F+      +  Y V +   +  F + + I L NL+IGLAVGDI ++   A+++   ++V     +E  +P
Sbjct:  244 LHLAVQSGDLDMIKMCLDSGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDI-VNAVDGNQETLLHRASLFDHHDLADYLISVGADINSTDSEGRSPLILATASASW-----NIVNLLLSKGAKVDIKDHLGRNFLH-LTVQQPYGLRNLRPEFLQMQHIKELVMDEDNDGCTPLHYACRQGAPVSVNNLLRFNVSVHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLS-DHNGWTALHHASMGGYTQTMKVIL--DTNLKCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRSKRWDECLQVFTHDSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQC----PLSMTKKVTPTQDVIYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMTLLVVKIQPGMAFNSTGI-----INETISTHEERINTLNSFPLKICMILVFLSSIFGYCKEVVQIFQQKRNYFLDYNNALEWVIYTTSMIFVLPLFLDIPAYMQWQCGAIAIFFYWMNFLLYLQRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDAFSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKLP 994          

HSP 2 Score: 107.071 bits (266), Expect = 2.999e-22
Identity = 91/341 (26.69%), Postives = 168/341 (49.27%), Query Frame = 3
Query:   69 ENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD--FCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFR---DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            E  I  +  L + +++  C L +A     ++++++ ++   C  +N     G T  H A+  N     K + S+  N N+ + ++ +PLH A+     +++KVL +  A NINL  ++  T ++  C      K+ S  +++L+ + A L       DY +     +   K  +E+I    E    S+    IN  +    SPLH AV  G+L++ ++ L++GA+ ++     C+ LH AA +G  DIVKL+++S   S       D N+ + +H A+   ++++  +LI +G D  + D  GR+ L+ A  S    I+  LL+K   V++ D +    LH
Sbjct:   48 EAFIKNRRKLSKYEDENLCLLHHAAAEGQVELMQLIINGSSCEALNVMDDYGNTPLHWAAEKNQVESVKFLLSQGANPNLRNRNMMAPLHIAVQGMYNEVIKVLTEHKATNINLEGENGNTALMSTCA-----KDNSEALQILLEKGAKLCKSNKWGDYPVHQAA-FSGAKRCMELILAYGEKTGYSREAH-INFVNHKKASPLHLAVQSGDLDMIKMCLDSGAHIDMMENAKCMALHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLADYLISVGADINSTDSEGRSPLILATASASWNIVNLLLSKGAKVDIKDHLGRNFLH 381          

HSP 3 Score: 75.485 bits (184), Expect = 1.873e-12
Identity = 65/264 (24.62%), Postives = 116/264 (43.94%), Query Frame = 3
Query:  537 IFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL--------TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS--PLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTL 1298
            + H+   + ++E+++ I    S     E +N  D    +PLH+A     +E  + LL  GANPN+   N   PLHIA +  + +++K+L      +      N ++A+       N E  + L+  G      +++G   +  AA SG    +E +L        ++E ++N  +  K + LH A   G L +++    SGA + D M       L  A +    ++VK  ++        +Y    GSS I N +  N++  L
Sbjct:   67 LLHHAAAEGQVELMQLIINGSS----CEALNVMDDYGNTPLHWAAEKNQVESVKFLLSQGANPNLRNRNMMAPLHIAVQGMYNEVIKVLTEHKATNINLEGENGNTALMSTCAKDNSEALQILLEKGAKLCKSNKWGDYPVHQAAFSGAKRCMELILAYGEKTGYSREAHINFVNHKKASPLHLAVQSGDLDMIKMCLDSGAHI-DMMENAKCMALHFAATQGATDIVKLMIS--------SY---TGSSDIVNAVDGNQETLL 314          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267E899 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025935g1 PE=3 SV=1)

HSP 1 Score: 376.711 bits (966), Expect = 1.067e-110
Identity = 244/783 (31.16%), Postives = 416/783 (53.13%), Query Frame = 3
Query:   81 NVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNI---RDYDMKTIFHYLVVKEKIEMIKWIYENISE-SQRIE-LINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRK----SECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA--EGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILII---MIVGIRNIILVMTLIVQKFRNL--FEFWTNCYF-ACNSLALIIISNPSKLPCLYEWEKVPGL--IFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMI-SLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLY 2369
            N+   L+ KD +G   + YA ++    +I+   +   DIN +  +  T  H AS + +      +     + N  D    +PLH   I+ + ++  +LLK G++    + +F TP   AC         + + +   +  ADL++    D  M+T  H+ V+   IE++ ++ +  +E  +  E ++N+ D  DK P+HYAV +GN E+   LL  GA+ ++       P+ +A + G +++V+ +   S K    +   RD +  +++ IAAE G+ E+F +L+  G D + KDE GR  LM +  SG   I   ++ K  +VN  D  + TALH A    +     V+ L  +GA L  N  G++P+D+A+ NN    ++ F +D +   VM YR +NG +H++ LI+ +      +L++ + E+ ++ MD N+ V Y F+ +D GPDDPAS   RY AL++M + +R++LL HP+   L++ KW      FF  D L + V++  +T FV  N   L    +D +TGC+  N +T      S +   +T+  LI+   +++GI +I LV+  +   F+ L  F+ + N +F        I +  P   PC Y W  + G+  + +++ L I+  +HLP +GI  +MFQ+V+ ++++VL  F     GFA  F I    Q PFK +  + +K+  M  GE D VTYF+S       T L+++  +FV+IM I L NL++G+AVGDI  I+ EA+++   +++    E+ETN+P   QR L+
Sbjct:  336 NIVQMLNIKDAEGNSAMHYAARSDKPSMIQGLKNCGADINIRNLENSTPLHAASALGHKESVMKLLELGADPNQQDKLGRTPLHSCAISKQEEICGILLKAGSDPQAADAEFMTPFHWACRR-------NQLNEAAHLADADLDVIAATDIQMRTGLHWAVLAGSIELVNYLLDKAAELPEGCETVLNASDAQDKYPVHYAVEVGNTELLVALLSRGADFHVVDMEGRTPVQLAVKMGCIELVQKIFEHSEKLKHQALKHRDMSGMTSLLIAAEQGHSELFDYLLEQGADFMVKDEIGRTALMLSVGSGHHEIASVMIDKGVDVNASDSSRNTALHYACKHEDSDASCVELLLKNGAQLQQNYNGETPVDVAVLNNNNPAIQLFFSDAHRNWVMTYRKENGYTHLDVLIQHSPQTAELILNQAMTESGADHMDKNFMVVYNFTDLDPGPDDPASAHRRYLALRTMVKRRRVELLEHPVCQALIESKW-AVARYFFWFDLLCYAVFLCCLTAFVADNGP-LPLWEIDTQTGCR--NYTTDGV--SSGTRQKLTNPTLILEKAIVIGILSIRLVIETVKVAFKRLQYFKNYENFFFIVLYIFTFIFVYPPDSEPCQYNW--ICGMFSVIMAWCLLIFQFEHLPKIGIYSLMFQQVIVSLVKVLVIFSFCIIGFALAFNIGMVAQTPFKYITYSILKTFDMTVGELDFVTYFISNSYGGMQTGLLVVFVIFVIIMPIGLMNLLVGIAVGDIDGISKEASLKLIDIRITLFYELETNLPRYFQRRLH 1103          

HSP 2 Score: 80.8777 bits (198), Expect = 1.198e-11
Identity = 76/337 (22.55%), Postives = 144/337 (42.73%), Query Frame = 3
Query:  219 KTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLA--------KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFL 1202
            K  FH+    N  ++  L+K+    ++  D+ L S LH+A+ ++++ +V  L++ GAN+N+  ++   P     + ++A  N  G +K L+                                      S R   +++ D   ++ LH A    + E+ Q+LL   ANP +   +    LH+ + + +L + ++L N +    C RD +  + + +AA     E+    + + K            KD  G + + +AARS    +I+ L     ++N+ +    T LH A+A GH + V  L   GA  +  +  G++PL     + QE +    L
Sbjct:  142 KLFFHSVREGNTDLVLMLIKNDSSLVSELDDQLMSGLHYAVKSDRVGVVAALIQAGANVNMKGREGSRP-----LHVAANFNQIGALKELL--------------------------------------SSRKVRVDATDDRGQTALHVACRRNHEEVVQILLGHKANPQLKELDGTSVLHLPSRRNNLQLCQILTNDTALISC-RDKHGVTPLMVAASESGEELLSHFVSVCKLSDKNIVQMLNIKDAEGNSAMHYAARSDKPSMIQGLKNCGADINIRNLENSTPLHAASALGHKESVMKLLELGADPNQQDKLGRTPLHSCAISKQEEICGILL 434          

HSP 3 Score: 63.5438 bits (153), Expect = 2.203e-6
Identity = 47/184 (25.54%), Postives = 86/184 (46.74%), Query Frame = 3
Query:  525 DMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAE 1076
            + + +F + V +   +++  + +N S      L++  D    S LHYAV    + +   L++AGAN N+       PLH+AA    +  +K LL+S +      D    +A+H+A    + E+ + L+G   +   K+  G + L   +R   L + + L      ++  D+  VT L  AA+E
Sbjct:  139 EQRKLFFHSVREGNTDLVLMLIKNDSS-----LVSELDDQLMSGLHYAVKSDRVGVVAALIQAGANVNMKGREGSRPLHVAANFNQIGALKELLSSRKVRVDATDDRGQTALHVACRRNHEEVVQILLGHKANPQLKELDGTSVLHLPSRRNNLQLCQILTNDTALISCRDKHGVTPLMVAASE 317          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267FPG6 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025935g2 PE=3 SV=1)

HSP 1 Score: 375.941 bits (964), Expect = 1.930e-110
Identity = 244/783 (31.16%), Postives = 417/783 (53.26%), Query Frame = 3
Query:   81 NVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNI---RDYDMKTIFHYLVVKEKIEMIKWIYENISE-SQRIE-LINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRK----SECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA--EGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILII---MIVGIRNIILVMTLIVQKFRNL--FEFWTNCYF-ACNSLALIIISNPSKLPCLYEWEKVPGL--IFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMI-SLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLY 2369
            N+   L+ KD +G   + YA ++    +I+   +   DIN +  +  T  H AS + +      +     + N  D    +PLH   I+ + ++  +LLK G++    + +F TP   AC         + + +   +  ADL++    D  M+T  H+ V+   IE++ ++ +  +E  +  E ++N+ D  DK P+HYAV +GN E+   LL  GA+ ++       P+ +A + G +++V+ +   S K    +   RD +  +++ IAAE G+ E+F++L+  G D + KDE GR  LM +  SG   I   ++ K  +VN  D  + TALH A    +     V+ L  +GA L  N  G++P+D+A+ NN    ++ F +D +   VM YR +NG +H++ LI+ +      +L++ + E+ ++ MD N+ V Y F+ +D GPDDPAS   RY AL++M + +R++LL HP+   L++ KW      FF  D L + V++  +T FV  N   L    +D +TGC+  N +T      S +   +T+  LI+   +++GI +I LV+  +   F+ L  F+ + N +F        I +  P   PC Y W  + G+  + +++ L I+  +HLP +GI  +MFQ+V+ ++++VL  F     GFA  F I    Q PFK +  + +K+  M  GE D VTYF+S       T L+++  +FV+IM I L NL++G+AVGDI  I+ EA+++   +++    E+ETN+P   QR L+
Sbjct:  336 NIVQMLNIKDAEGNSAMHYAARSDKPSMIQGLKNCGADINIRNLENSTPLHAASALGHKESVIKLLELGADPNQQDKLGRTPLHSCAISKQEEICGILLKAGSDPQAADAEFMTPFHWACRR-------NQLNEAAHLADADLDVIAATDIQMRTGLHWAVLAGSIELVNYLLDKAAELPEGCETVLNASDAQDKYPVHYAVEVGNTELLVALLSRGADFHVVDMEGRTPVQLAVKMGCIELVQNIFEHSEKLKHQALKHRDMSGMTSLLIAAEQGHSELFEYLLEQGADFMVKDEIGRTALMLSVGSGHHEIASVMIDKGVDVNASDSSRNTALHYACKHEDSDASCVELLLKNGAQLQQNYNGETPVDVAVLNNNNPAIQLFFSDAHRTWVMTYRKENGYTHLDVLIQHSPQTAELILNQAMTESGADHMDKNFMVVYNFTDLDPGPDDPASAHRRYLALRTMVKRRRVELLEHPVCQALIESKW-AVARYFFWFDLLCYAVFLCCLTAFVADNGP-LPLWEIDTQTGCR--NYTTDGV--SSGTRQKLTNPTLILEKAIVIGILSIRLVIETVKVAFKRLQYFKNYENFFFIVLYIFTFIFVYPPDSEPCQYNW--ICGMFSVIMAWCLLIFQFEHLPKIGIYSLMFQQVIVSLVKVLVIFSFCIIGFALAFNIGMVAQTPFKYITYSILKTFDMTVGELDFVTYFISNSYGGMQTGLLVVFVIFVIIMPIGLMNLLVGIAVGDIDGISKEASLKLIDIRITLFYELETNLPRYFQRRLH 1103          

HSP 2 Score: 79.7221 bits (195), Expect = 2.603e-11
Identity = 76/337 (22.55%), Postives = 144/337 (42.73%), Query Frame = 3
Query:  219 KTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLA--------KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFL 1202
            K  FH+    N  ++  L+K+    ++  D+ L S LH+A+ ++++ +V  L++ GAN+N+  ++   P     + ++A  N  G +K L+                                      S R   +++ D   ++ LH A    + E+ Q+LL   ANP +   +    LH+ + + +L + ++L N +    C RD +  + + +AA     E+    + + K            KD  G + + +AARS    +I+ L     ++N+ +    T LH A+A GH + V  L   GA  +  +  G++PL     + QE +    L
Sbjct:  142 KLFFHSVREGNTDLVLMLIKNDSSLVSELDDQLMSGLHYAVKSDRVGVVAALIQAGANVNMKGREGSRP-----LHVAANFNQIGALKELL--------------------------------------SSRKVRVDATDDRGQTALHVACRRNHEEVVQILLGHKANPQLKELDGTSVLHLPSRRNNLQLCQILTNDTALISC-RDKHGVTPLMVAASESGEELLSHFVSVCKLSDKNIVQMLNIKDAEGNSAMHYAARSDKPSMIQGLKNCGADINIRNLENSTPLHAASALGHKESVIKLLELGADPNQQDKLGRTPLHSCAISKQEEICGILL 434          

HSP 3 Score: 63.929 bits (154), Expect = 2.058e-6
Identity = 47/184 (25.54%), Postives = 86/184 (46.74%), Query Frame = 3
Query:  525 DMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAE 1076
            + + +F + V +   +++  + +N S      L++  D    S LHYAV    + +   L++AGAN N+       PLH+AA    +  +K LL+S +      D    +A+H+A    + E+ + L+G   +   K+  G + L   +R   L + + L      ++  D+  VT L  AA+E
Sbjct:  139 EQRKLFFHSVREGNTDLVLMLIKNDSS-----LVSELDDQLMSGLHYAVKSDRVGVVAALIQAGANVNMKGREGSRPLHVAANFNQIGALKELLSSRKVRVDATDDRGQTALHVACRRNHEEVVQILLGHKANPQLKELDGTSVLHLPSRRNNLQLCQILTNDTALISCRDKHGVTPLMVAASE 317          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267G9R9 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000210g4 PE=3 SV=1)

HSP 1 Score: 355.14 bits (910), Expect = 2.215e-103
Identity = 240/788 (30.46%), Postives = 408/788 (51.78%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS-GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGH----LDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAA--AEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILII---MIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFAC-NSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLI-MISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFI 2417
            LD +D++G   + +A +N     I+I  +  V+++ +  +     H A+ + +      +       N+ D +  +PLH  II  +L++   LL  GA     ++DF TP+  AC      + L+    +LL ++   L++ D  M+T  H+ V+   +++++ +     +S   E  N+ D  DK P+HYAV + + ++ ++LL  GA+ +           +A + GH     DI  L     R++   RD    +++ IAAE+G+  +  FL+  G D  AKDEFGR  LM +   G   I+  ++ K  +VN  D  + TALH A   A    + ++ L ++GA L  N  G++P+D+A+ NN    +    +D     VM+YR D G SH++ LI+ +    L +L++ + ++ ++  + N+ V + F  +D GPDDPAS   RY  L++M E +R  LL+HPL A LL+ KW  F   FF  DF  + V++  +T FV +++  L    VD  TGC+  +SS  + +++      +T+  LI+   +++G   + LV+  I   F+ L   + + N +F     L  + I  P   PC+  W      + L++ L I+  +HLP  GI  +MFQKV+ ++++VL  F     GF   F I    Q PFK    + +K+  M  GE + VTYF+SG       +L+ +  +FVV+M I L NL++G+AVGDI  I+ EA ++  ++++  + E+ETN+P   QR LY   +   P +   R +
Sbjct:  258 LDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVNLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRAADEDFMTPLHWACR-----RGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSD--ESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRDLRGVTSLLIAAEHGHTRVLHFLLEQGADFTAKDEFGRTALMLSCGGGHSDIVATMIDKGVDVNASDANRNTALHYACKHAGSSARSLELLLSNGAQLQQNYNGETPVDVAVLNNNGPAISLLFSDAYCDWVMSYRKDTGMSHMDILIQHSPQTALTILNRSMTKSRADNFEKNFMVTHNFFYLDPGPDDPASKPNRYLGLRTMVERQREDLLKHPLCAALLEYKW-SFTRYFFWCDFFFYAVFLCCLTAFV-ADQGPLPISLVDANTGCRNTSSSGGSGQRQ-----KLTNGRLILEKSVVIGFLCVRLVLEFIKLVFKRLQYLKNYENLFFVTLYILTFVFIYPPDSEPCIDNWIFGIFSVILAWCLLIFQFEHLPVTGIYSLMFQKVIISLVKVLLIFAFFIIGFGLAFNIGLVSQTPFKYPMYSILKTFDMTVGEMEFVTYFISGTSGRMQLALLVVFVIFVVVMPIGLMNLLVGIAVGDIEGISKEAGLRLVEIRINLVYEMETNLPRYFQRRLYRRFLQFYPNRTGNRLV 1031          

HSP 2 Score: 80.1073 bits (196), Expect = 1.854e-11
Identity = 98/428 (22.90%), Postives = 176/428 (41.12%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYP-ILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN--------------------INLC-------------NKDFQTPILLACMEISA--LKNLSGIIKLLIIEKAD--LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLA-KDEFGRNTLMFAARSGCLPIIEYLLTK----ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDN 1247
            LDE+   G   L YAV+   +D++ + +    ++N K  +G    H A+  N    L +++KSR   ++  D+   + LH A   N    VK+LL   AN                    + LC             +K   TP+++A  E S   L     + K      A   L+++D +  +  H+    +K   I+ + E+         ++ Q++   +P+H A ++G+ E    L+  GA PN+   N   PLH       L+I   LL +  +S    D +  + +H A   G  +  + L+ +   CL+  D   R  L +A  +  + ++E LL +    + + N  D      +H A      Q+V+ L T GA   + +  G++   +A+    E +V+       +    A RH +
Sbjct:   86 LDEQLMSG---LHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVAANFNQSDALREILKSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGTSVLHLPSRRNNLELCQMLTTDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKTTHGAAGRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVN------LDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRA-ADEDFMTPLHWACRRGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSDESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRD 503          

HSP 3 Score: 74.3294 bits (181), Expect = 1.262e-9
Identity = 80/326 (24.54%), Postives = 140/326 (42.94%), Query Frame = 3
Query:  216 GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAAND---CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAI--LSDNMFGQSPLDIAISNNQ 1178
             +  FH+    N   +  L+K     ++  D  L S LH+A+  N++DMV +L++ GAN+N+  ++   PI +A    +       + ++L   +  ++  D   +T  H    +     +K +  + + SQ  EL  +      S LH      NLE+CQ+L     +  +    D     PL +AA +G  D++   ++  +K+            H AA              G+    +D  G +++ FAA++     IE L     N++L +  +   +H AA  GH + V  L   GA+  LSD   G++PL   I   Q
Sbjct:   57 ARRFFHSVREGNSDQVSLLLKCNSSLVSKLDEQLMSGLHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVA----ANFNQSDALREILKSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGT------SVLHLPSRRNNLELCQMLT---TDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKT-----------THGAA--------------GRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVNLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKN-GRTPLHCCIIARQ 343          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267EGL5 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000210g1 PE=3 SV=1)

HSP 1 Score: 354.755 bits (909), Expect = 2.948e-103
Identity = 240/788 (30.46%), Postives = 408/788 (51.78%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS-GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGH----LDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAA--AEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILII---MIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFAC-NSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLI-MISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFI 2417
            LD +D++G   + +A +N     I+I  +  V+++ +  +     H A+ + +      +       N+ D +  +PLH  II  +L++   LL  GA     ++DF TP+  AC      + L+    +LL ++   L++ D  M+T  H+ V+   +++++ +     +S   E  N+ D  DK P+HYAV + + ++ ++LL  GA+ +           +A + GH     DI  L     R++   RD    +++ IAAE+G+  +  FL+  G D  AKDEFGR  LM +   G   I+  ++ K  +VN  D  + TALH A   A    + ++ L ++GA L  N  G++P+D+A+ NN    +    +D     VM+YR D G SH++ LI+ +    L +L++ + ++ ++  + N+ V + F  +D GPDDPAS   RY  L++M E +R  LL+HPL A LL+ KW  F   FF  DF  + V++  +T FV +++  L    VD  TGC+  +SS  + +++      +T+  LI+   +++G   + LV+  I   F+ L   + + N +F     L  + I  P   PC+  W      + L++ L I+  +HLP  GI  +MFQKV+ ++++VL  F     GF   F I    Q PFK    + +K+  M  GE + VTYF+SG       +L+ +  +FVV+M I L NL++G+AVGDI  I+ EA ++  ++++  + E+ETN+P   QR LY   +   P +   R +
Sbjct:  258 LDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVNLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRAADEDFMTPLHWACR-----RGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSD--ESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRDLRGVTSLLIAAEHGHTRVLHFLLEQGADFTAKDEFGRTALMLSCGGGHSDIVATMIDKGVDVNASDANRNTALHYACKHAGSSARSLELLLSNGAQLQQNYNGETPVDVAVLNNNGPAISLLFSDAYCDWVMSYRKDTGMSHMDILIQHSPQTALTILNRSMTKSGADNFEKNFMVTHNFFYLDPGPDDPASKPNRYLGLRTMVERQREDLLKHPLCAALLEYKW-SFTRYFFWCDFFFYAVFLCCLTAFV-ADQGPLPISLVDANTGCRNTSSSGGSGQRQ-----KLTNGRLILEKSVVIGFLCVRLVLEFIKLVFKRLQYLKNYENLFFVTLYILTFVFIYPPDSEPCIDNWIFGIFSVILAWCLLIFQFEHLPVTGIYSLMFQKVIISLVKVLLIFAFFIIGFGLAFNIGLVSQTPFKYPMYSILKTFDMTVGEMEFVTYFISGTSGRMQLALLVVFVIFVVVMPIGLMNLLVGIAVGDIEGISKEAGLRLVEIRINLVYEMETNLPRYFQRRLYRRFLQFYPNRTGNRLV 1031          

HSP 2 Score: 78.9518 bits (193), Expect = 4.697e-11
Identity = 98/428 (22.90%), Postives = 175/428 (40.89%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYP-ILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN--------------------INLC-------------NKDFQTPILLACMEISA--LKNLSGIIKLLIIEKAD--LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLA-KDEFGRNTLMFAARSGCLPIIEYLLTK----ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDN 1247
            LDE+   G   L YAV+   +D++ + +    ++N K  +G    H A+  N    L +L+ SR   ++  D+   + LH A   N    VK+LL   AN                    + LC             +K   TP+++A  E S   L     + K      A   L+++D +  +  H+    +K   I+ + E+         ++ Q++   +P+H A ++G+ E    L+  GA PN+   N   PLH       L+I   LL +  +S    D +  + +H A   G  +  + L+ +   CL+  D   R  L +A  +  + ++E LL +    + + N  D      +H A      Q+V+ L T GA   + +  G++   +A+    E +V+       +    A RH +
Sbjct:   86 LDEQLMSG---LHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVAANFNQSDALRELLNSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGTSVLHLPSRRNNLELCQMLTTDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKTTHGAAGRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVN------LDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRA-ADEDFMTPLHWACRRGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSDESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRD 503          

HSP 3 Score: 74.7146 bits (182), Expect = 8.597e-10
Identity = 81/326 (24.85%), Postives = 140/326 (42.94%), Query Frame = 3
Query:  216 GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAAND---CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAI--LSDNMFGQSPLDIAISNNQ 1178
             +  FH+    N   +  L+K     ++  D  L S LH+A+  N++DMV +L++ GAN+N+  ++   PI +A    +       + +LL   +  ++  D   +T  H    +     +K +  + + SQ  EL  +      S LH      NLE+CQ+L     +  +    D     PL +AA +G  D++   ++  +K+            H AA              G+    +D  G +++ FAA++     IE L     N++L +  +   +H AA  GH + V  L   GA+  LSD   G++PL   I   Q
Sbjct:   57 ARRFFHSVREGNSDQVSLLLKCNSSLVSKLDEQLMSGLHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVA----ANFNQSDALRELLNSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGT------SVLHLPSRRNNLELCQMLT---TDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKT-----------THGAA--------------GRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGVNLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKN-GRTPLHCCIIARQ 343          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Match: A0A267H4S2 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000210g3 PE=3 SV=1)

HSP 1 Score: 353.984 bits (907), Expect = 5.492e-103
Identity = 239/788 (30.33%), Postives = 408/788 (51.78%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLS-GIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGH----LDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAA--AEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILII---MIVGIRNIILVMTLIVQKFRNL--FEFWTNCYFAC-NSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLI-MISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFI 2417
            LD +D++G   + +A +N     I+I  +   +++ +  +     H A+ + +      +       N+ D +  +PLH  II  +L++   LL  GA     ++DF TP+  AC      + L+    +LL ++   L++ D  M+T  H+ V+   +++++ +     +S   E  N+ D  DK P+HYAV + + ++ ++LL  GA+ +           +A + GH     DI  L     R++   RD    +++ IAAE+G+  +  FL+  G D  AKDEFGR  LM +   G   I+  ++ K  +VN  D  + TALH A   A    + ++ L ++GA L  N  G++P+D+A+ NN    +    +D     VM+YR D G SH++ LI+ +    L +L++ + ++ ++ ++ N+ V + F  +D GPDDPAS   RY  L++M E +R  LL+HPL A LL+ KW  F   FF  DF  + V++  +T FV +++  L    VD  TGC+  +SS  + +++      +T+  LI+   +++G   + LV+  I   F+ L   + + N +F     L  + I  P   PC+  W      + L++ L I+  +HLP  GI  +MFQKV+ ++++VL  F     GF   F I    Q PFK    + +K+  M  GE + VTYF+SG       +L+ +  +FVV+M I L NL++G+AVGDI  I+ EA ++  ++++  + E+ETN+P   QR LY   +   P +   R +
Sbjct:  258 LDMQDSEGNSSVHFAAKNDKFLAIEILSEHGANLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRAADEDFMTPLHWACR-----RGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSD--ESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRDLRGVTSLLIAAEHGHTRVLHFLLEQGADFTAKDEFGRTALMLSCGGGHSDIVATMIDKGVDVNASDVNRNTALHYACKHAGSSARSLELLLSNGAQLQQNYNGETPVDVAVLNNNGPAISLLFSDAYCDWVMSYRKDTGMSHMDILIQHSPQTALTILNRSMTKSGADNLEKNFMVTHNFFYLDPGPDDPASKPNRYLGLRTMVERQREDLLKHPLCAALLEYKW-SFTRYFFWCDFFFYAVFLCCLTAFV-ADQGPLPISLVDANTGCRNTSSSGGSGQRQ-----KLTNGRLILEKSVVIGFLCVRLVLEFIKLVFKRLQYLKNYENLFFVTLYILTFVFIYPPDSEPCIDNWIFGIFSVILAWCLLIFQFEHLPVTGIYSLMFQKVIISLVKVLLIFAFFIIGFGLAFNIGLVSQTPFKYPMYSILKTFDMTVGEMEFVTYFISGTSGRMQLALLVVFVIFVVVMPIGLMNLLVGIAVGDIEGISKEAGLRLVEIRINLVYEMETNLPRYFQRRLYRRFLQFYPNRTGNRLV 1031          

HSP 2 Score: 82.4185 bits (202), Expect = 3.308e-12
Identity = 102/442 (23.08%), Postives = 185/442 (41.86%), Query Frame = 3
Query:   54 SKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYP-ILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN--------------------INLC-------------NKDFQTPILLACMEISA--LKNLSGIIKLLIIEKAD--LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLA-KDEFGRNTLMFAARSGCLPIIEYLLTK----ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDN 1247
            S+L+K N  ++ + LDE+   G   L YAV+   +D++ + +    ++N K  +G    H A+  N    L +L+ SR   ++  D+   + LH A   N    VK+LL   AN                    + LC             +K   TP+++A  E S   L     + K      A   L+++D +  +  H+    +K   I+ + E+ +       ++ Q++   +P+H A ++G+ E    L+  GA PN+   N   PLH       L+I   LL +  +S    D +  + +H A   G  +  + L+ +   CL+  D   R  L +A  +  + ++E LL +    + + N  D      +H A      Q+V+ L T GA   + +  G++   +A+    E +V+       +    A RH +
Sbjct:   73 SRLLKCNS-SLVSKLDEQLMSG---LHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVAANFNQSDALRELLNSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGTSVLHLPSRRNNLELCQMLTTDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKTTHGAAGRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGAN------LDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKNGRTPLHCCIIARQLEICDTLLLAGAQSRA-ADEDFMTPLHWACRRGLTQAARRLLRVDPGCLSLVDIHMRTGLHWAVLACAVDLVELLLAQAGDSDESANASDFHDKYPVHYAVEMNDSQLVRILLTHGADFHAVDTEGRTATQLAVKLGHEGLVRDICGLGQYIKRQALRHRD 503          

HSP 3 Score: 77.411 bits (189), Expect = 1.252e-10
Identity = 81/326 (24.85%), Postives = 141/326 (43.25%), Query Frame = 3
Query:  216 GKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAAND---CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAI--LSDNMFGQSPLDIAISNNQ 1178
             +  FH+    N   + +L+K     ++  D  L S LH+A+  N++DMV +L++ GAN+N+  ++   PI +A    +       + +LL   +  ++  D   +T  H    +     +K +  + + SQ  EL  +      S LH      NLE+CQ+L     +  +    D     PL +AA +G  D++   ++  +K+            H AA              G+    +D  G +++ FAA++     IE L     N++L +  +   +H AA  GH + V  L   GA+  LSD   G++PL   I   Q
Sbjct:   57 ARRFFHSVREGNSDQVSRLLKCNSSLVSKLDEQLMSGLHYAVKCNRVDMVSLLMQAGANVNIKGREGSRPIHVA----ANFNQSDALRELLNSRRTRVDATDDRGQTALHVACRRNHEPSVKILLSHKANSQLKELDGT------SVLHLPSRRNNLELCQMLT---TDTGLITCKDKYGVTPLMVAAAEGSEDLLNYFISVCKKT-----------THGAA--------------GRILDMQDSEGNSSVHFAAKNDKFLAIEILSEHGANLDLQNLEQSAPIHLAATLGHRESVAKLIHLGALPNLSDKN-GRTPLHCCIIARQ 343          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: trpa1b (transient receptor potential cation channel subfamily A member 1 [Source:NCBI gene;Acc:103042231])

HSP 1 Score: 209.92 bits (533), Expect = 8.129e-56
Identity = 199/802 (24.81%), Postives = 374/802 (46.63%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHD---QMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD----LVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRP 2393
            +E GI     ++  D      L  AV+  N ++IK+C+     ++ +Q D  T  H A         K+M     +  +I IN+ D    +PLH A + + +++ + L+  GA+I+  +    +P+LLA    SA K +S     L+++ AD  ++D   +   H ++ + K   +K + EN+ +S+ + EL++ +D    +PLHYA  +G  +  + +L         +      LH AAE G L+    LL +   S    +  E+  + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    +  LL    NV L D   +   TALH AA  GH+  V+ L   GA+   N  G S L  A+ N ++ V    + ++     ++ ++   G    + +LI+   + T  LLD C++E+  +  + NY + Y F  + A          D +   +   AL +M +Y RL+LL HP+  K L++KW  +G     ++  ++L+ ++ +TY ++ N +   + SV+         + T  S      +  ++  I++++++ +  I   +  + Q+    F   TN     ++++ ++   P    +   + WE     I  ++  F+ Y Q     GI ++MF +++KT+I  +  F  +   F   FY +   +  F ++ L+  ++  M  GE +    L+  +  G+  +   S  +  LFV++M I L NL+IGLAVGDI ++   AT++   +++     +E  +P     Y Y   + D+P
Sbjct:  212 EEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEI-INIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLA-TTCSAWKTVS----FLLLKGADFRVKDKAGRNFLHLVIFQPK--GLKNLPENLLQSKAVRELMSDEDYEGCTPLHYACKLGIHDSVKNMLSQKICLGRKSKQKKSGLHFAAEYGRLNTCIRLLETISDSRLLNEGEENGLTPLHLASRGGHTKVVELLLRRGA-LFQSDYKGWSCLHHAAAEGYTQTMVILLA--ANVKLLDYTNEEGNTALHLAARAGHVAAVKLLLNRGALFMLNKNGASFLHEAVRNGKKEVANAAIENERSSEALSLFKKGPGFRCVVLDLIEFLPESTKHLLDLCMVESEHDPNNINYHITYNFKWLQAPILLKKYAKTDKSMNFQPLAALNAMVQYNRLELLTHPVCQKYLEMKWKAYGSKVHLLNLAVYLLGLLPLTYLII-NLKPTQNTSVN-------GTTVTMASVSFRTQSYMISVCIVMVLVMNVYAICKELVQLAQQRLIYFMDSTNVSDWASAISSLVFVIPLFFSVDNKWHWEAGALAILFAWINFLLYFQRFERFGIYVVMFGEIIKTLISTIVLFVFLLLAFGLSFYALMLHRVEFNSISLSLAQTFVMTVGELNYQNTLLEAYEQGQLAFPEISYFIFVLFVLLMPILLMNLMIGLAVGDIAEVQRNATLKRIAMQINLHTNLEEKLP-----YWYMKRV-DKP 988          

HSP 2 Score: 73.9442 bits (180), Expect = 6.072e-13
Identity = 80/332 (24.10%), Postives = 142/332 (42.77%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFC--VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFG-ANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D  G   + +A  + NL +I++ +      ++NT+   G+T  H A   N      L+     + N+    L +PLH AI     ++V++LL     +++L      TP ++AC       N    +++L    A L     +  + + T+  +   KE +E+I    E +  +     IN  D    SPLH AV  GN E+ ++ +  GA  +    +    LH A  +G  + VK++L    + E     RD    + +H A    + E+ ++L+  G D    D  G + L+ A        + +LL K  +  + D+     LH
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGPEELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACS-----NNNCQALQILFKYNAKLCHQNKLGHFAIHTV-AFAGAKEAMEVILQKGEEMGITTSGH-INYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEIINIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLATTCSAWKTVSFLLLKGADFRVKDKAGRNFLH 370          

HSP 3 Score: 73.559 bits (179), Expect = 7.643e-13
Identity = 61/242 (25.21%), Postives = 104/242 (42.98%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFLN 1205
            L  RD       H+      + +I+ I   +      E +N+QD   ++PLH+AV       C +LLE GA+PNI + +   PLH+A    H ++V+LLL   R          ++   +A  N N +  + L         +++ G    +T+ FA     + +I     E  +T   ++N  D+ K + LH A   G+ +V++   + GA +      +S  L  A +      VK  L 
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGP----EELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACSNNNCQALQILFKYNAKLCHQNKLGHFAIHTVAFAGAKEAMEVILQKGEEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQ 283          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: trpa1b (transient receptor potential cation channel subfamily A member 1 [Source:NCBI gene;Acc:103042231])

HSP 1 Score: 209.92 bits (533), Expect = 9.193e-56
Identity = 199/802 (24.81%), Postives = 374/802 (46.63%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHD---QMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD----LVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRP 2393
            +E GI     ++  D      L  AV+  N ++IK+C+     ++ +Q D  T  H A         K+M     +  +I IN+ D    +PLH A + + +++ + L+  GA+I+  +    +P+LLA    SA K +S     L+++ AD  ++D   +   H ++ + K   +K + EN+ +S+ + EL++ +D    +PLHYA  +G  +  + +L         +      LH AAE G L+    LL +   S    +  E+  + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    +  LL    NV L D   +   TALH AA  GH+  V+ L   GA+   N  G S L  A+ N ++ V    + ++     ++ ++   G    + +LI+   + T  LLD C++E+  +  + NY + Y F  + A          D +   +   AL +M +Y RL+LL HP+  K L++KW  +G     ++  ++L+ ++ +TY ++ N +   + SV+         + T  S      +  ++  I++++++ +  I   +  + Q+    F   TN     ++++ ++   P    +   + WE     I  ++  F+ Y Q     GI ++MF +++KT+I  +  F  +   F   FY +   +  F ++ L+  ++  M  GE +    L+  +  G+  +   S  +  LFV++M I L NL+IGLAVGDI ++   AT++   +++     +E  +P     Y Y   + D+P
Sbjct:  212 EEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEI-INIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLATT-CSAWKTVS----FLLLKGADFRVKDKAGRNFLHLVIFQPK--GLKNLPENLLQSKAVRELMSDEDYEGCTPLHYACKLGIHDSVKNMLSQKICLGRKSKQKKSGLHFAAEYGRLNTCIRLLETISDSRLLNEGEENGLTPLHLASRGGHTKVVELLLRRGA-LFQSDYKGWSCLHHAAAEGYTQTMVILLA--ANVKLLDYTNEEGNTALHLAARAGHVAAVKLLLNRGALFMLNKNGASFLHEAVRNGKKEVANAAIENERSSEALSLFKKGPGFRCVVLDLIEFLPESTKHLLDLCMVESEHDPNNINYHITYNFKWLQAPILLKKYAKTDKSMNFQPLAALNAMVQYNRLELLTHPVCQKYLEMKWKAYGSKVHLLNLAVYLLGLLPLTYLII-NLKPTQNTSVN-------GTTVTMASVSFRTQSYMISVCIVMVLVMNVYAICKELVQLAQQRLIYFMDSTNVSDWASAISSLVFVIPLFFSVDNKWHWEAGALAILFAWINFLLYFQRFERFGIYVVMFGEIIKTLISTIVLFVFLLLAFGLSFYALMLHRVEFNSISLSLAQTFVMTVGELNYQNTLLEAYEQGQLAFPEISYFIFVLFVLLMPILLMNLMIGLAVGDIAEVQRNATLKRIAMQINLHTNLEEKLP-----YWYMKRV-DKP 988          

HSP 2 Score: 73.9442 bits (180), Expect = 5.858e-13
Identity = 80/332 (24.10%), Postives = 142/332 (42.77%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFC--VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFG-ANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D  G   + +A  + NL +I++ +      ++NT+   G+T  H A   N      L+     + N+    L +PLH AI     ++V++LL     +++L      TP ++AC       N    +++L    A L     +  + + T+  +   KE +E+I    E +  +     IN  D    SPLH AV  GN E+ ++ +  GA  +    +    LH A  +G  + VK++L    + E     RD    + +H A    + E+ ++L+  G D    D  G + L+ A        + +LL K  +  + D+     LH
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGPEELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACS-----NNNCQALQILFKYNAKLCHQNKLGHFAIHTV-AFAGAKEAMEVILQKGEEMGITTSGH-INYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEIINIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLATTCSAWKTVSFLLLKGADFRVKDKAGRNFLH 370          

HSP 3 Score: 73.559 bits (179), Expect = 7.499e-13
Identity = 61/242 (25.21%), Postives = 104/242 (42.98%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFLN 1205
            L  RD       H+      + +I+ I   +      E +N+QD   ++PLH+AV       C +LLE GA+PNI + +   PLH+A    H ++V+LLL   R          ++   +A  N N +  + L         +++ G    +T+ FA     + +I     E  +T   ++N  D+ K + LH A   G+ +V++   + GA +      +S  L  A +      VK  L 
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGP----EELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACSNNNCQALQILFKYNAKLCHQNKLGHFAIHTVAFAGAKEAMEVILQKGEEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQ 283          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: trpa1b (transient receptor potential cation channel subfamily A member 1 [Source:NCBI gene;Acc:103042231])

HSP 1 Score: 209.92 bits (533), Expect = 9.193e-56
Identity = 199/802 (24.81%), Postives = 374/802 (46.63%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHD---QMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA-YRHDNG-SSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDA-------GPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNP--SKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD----LVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRP 2393
            +E GI     ++  D      L  AV+  N ++IK+C+     ++ +Q D  T  H A         K+M     +  +I IN+ D    +PLH A + + +++ + L+  GA+I+  +    +P+LLA    SA K +S     L+++ AD  ++D   +   H ++ + K   +K + EN+ +S+ + EL++ +D    +PLHYA  +G  +  + +L         +      LH AAE G L+    LL +   S    +  E+  + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    +  LL    NV L D   +   TALH AA  GH+  V+ L   GA+   N  G S L  A+ N ++ V    + ++     ++ ++   G    + +LI+   + T  LLD C++E+  +  + NY + Y F  + A          D +   +   AL +M +Y RL+LL HP+  K L++KW  +G     ++  ++L+ ++ +TY ++ N +   + SV+         + T  S      +  ++  I++++++ +  I   +  + Q+    F   TN     ++++ ++   P    +   + WE     I  ++  F+ Y Q     GI ++MF +++KT+I  +  F  +   F   FY +   +  F ++ L+  ++  M  GE +    L+  +  G+  +   S  +  LFV++M I L NL+IGLAVGDI ++   AT++   +++     +E  +P     Y Y   + D+P
Sbjct:  212 EEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEI-INIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLATT-CSAWKTVS----FLLLKGADFRVKDKAGRNFLHLVIFQPK--GLKNLPENLLQSKAVRELMSDEDYEGCTPLHYACKLGIHDSVKNMLSQKICLGRKSKQKKSGLHFAAEYGRLNTCIRLLETISDSRLLNEGEENGLTPLHLASRGGHTKVVELLLRRGA-LFQSDYKGWSCLHHAAAEGYTQTMVILLA--ANVKLLDYTNEEGNTALHLAARAGHVAAVKLLLNRGALFMLNKNGASFLHEAVRNGKKEVANAAIENERSSEALSLFKKGPGFRCVVLDLIEFLPESTKHLLDLCMVESEHDPNNINYHITYNFKWLQAPILLKKYAKTDKSMNFQPLAALNAMVQYNRLELLTHPVCQKYLEMKWKAYGSKVHLLNLAVYLLGLLPLTYLII-NLKPTQNTSVN-------GTTVTMASVSFRTQSYMISVCIVMVLVMNVYAICKELVQLAQQRLIYFMDSTNVSDWASAISSLVFVIPLFFSVDNKWHWEAGALAILFAWINFLLYFQRFERFGIYVVMFGEIIKTLISTIVLFVFLLLAFGLSFYALMLHRVEFNSISLSLAQTFVMTVGELNYQNTLLEAYEQGQLAFPEISYFIFVLFVLLMPILLMNLMIGLAVGDIAEVQRNATLKRIAMQINLHTNLEEKLP-----YWYMKRV-DKP 988          

HSP 2 Score: 73.9442 bits (180), Expect = 5.858e-13
Identity = 80/332 (24.10%), Postives = 142/332 (42.77%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFC--VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFG-ANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADL----NIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---CFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALH 1061
            L  +D  G   + +A  + NL +I++ +      ++NT+   G+T  H A   N      L+     + N+    L +PLH AI     ++V++LL     +++L      TP ++AC       N    +++L    A L     +  + + T+  +   KE +E+I    E +  +     IN  D    SPLH AV  GN E+ ++ +  GA  +    +    LH A  +G  + VK++L    + E     RD    + +H A    + E+ ++L+  G D    D  G + L+ A        + +LL K  +  + D+     LH
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGPEELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACS-----NNNCQALQILFKYNAKLCHQNKLGHFAIHTV-AFAGAKEAMEVILQKGEEMGITTSGH-INYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQPYERMEEIINIRDGANQTPLHRATLFDHVELAEYLVSKGADIDCIDCKGLSPLLLATTCSAWKTVSFLLLKGADFRVKDKAGRNFLH 370          

HSP 3 Score: 73.559 bits (179), Expect = 7.499e-13
Identity = 61/242 (25.21%), Postives = 104/242 (42.98%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPII-----EYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFLN 1205
            L  RD       H+      + +I+ I   +      E +N+QD   ++PLH+AV       C +LLE GA+PNI + +   PLH+A    H ++V+LLL   R          ++   +A  N N +  + L         +++ G    +T+ FA     + +I     E  +T   ++N  D+ K + LH A   G+ +V++   + GA +      +S  L  A +      VK  L 
Sbjct:   46 LKSRDECGAGPIHHAAASANLAVIRLIVTVVGP----EELNTQDDHGRTPLHWAVEKNQEGSCSLLLELGADPNILSLSLTAPLHLAISLEHNNLVQLLLLCDRTDVDLEGDLGNTPAMVACSNNNCQALQILFKYNAKLCHQNKLGHFAIHTVAFAGAKEAMEVILQKGEEMGITTSGHINYLDKSKSSPLHLAVRGGNTEVIKLCISKGAKVDQQQCDKSTALHFACTQGATEAVKIMLQ 283          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: trpa1a (transient receptor potential cation channel subfamily A member 1-like [Source:NCBI gene;Acc:103022174])

HSP 1 Score: 202.986 bits (515), Expect = 1.970e-53
Identity = 190/797 (23.84%), Postives = 368/797 (46.17%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLID------------AGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPN---VTSNILIIMIVGIRNIILVMTLIVQKFRNLFEF---WTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLI--FLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEF----DLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G+++++ ++  D      L  AV+  NLD+I++C+     IN +Q D  T  H A         KLM     +  DI IN+ D    +PLH A I +  ++ + L+  GA+I+  +    +P+LLA     A +     + LL+   A+L +RD       H  +++ +   +K +   + + + + EL+N +D    +PLHYA  +G  +  + +L    + +  +      LH AAE G ++    LL     +    + +E   + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    +E LL   +N+ L D++     TALH AA  GH+  V+ L   GA    N    S L  A+ N ++ V    ++ D  +  +     N +    + ++I+   +    LLD CI E+  +     Y ++Y F  +                DD   ++    AL +M E+ R++LL HP+  K L++KW  +GM    ++ +++ + ++ +TY +           V++     +N + T  +   ++       +TS + +++ +   N   V   IVQ F+   ++    +N     +++  ++   P  L     W    G +   +S+   + YLQ     GI ++MF+++ +T++ ++  F  +   FA  FY +  +Q  F  + L+ M++  MM GE     + +  ++S +  + + +  +   FV+++ I L NL+IGLAVGDI ++ + A ++   +++     +E  +P
Sbjct:  204 QEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRVCDI-INITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLA-TSCGAWRT----VNLLLSHGAELKVRDKAGCNFLHLAILQPR--GLKNLPTEVLQHESVRELLNDEDSEGCTPLHYACRLGIPDSVKNMLGLEVSLDQKSKQKKSALHFAAEYGRINTCHRLLEPMTDTRLLNEGDEKGMTPLHLASRGGHVKVVELLLRKGA-LFHSDYKGWSCLHHAAAEGFTQTMETLLI--SNIKLLDKIDGDGNTALHLAARAGHVAAVRQLLCRGAQFVLNKGDASFLHEAVHNGRKEVTSAVIDSDRCEEAITTFKVNSAKRCIVLDMIEFLPESYKHLLDTCIKESDDDVNSCTYNIEYNFQWLQHTNHRIQNTDNIEEDDDYKPLA----ALNAMVEFNRVELLTHPVCKKFLEMKWKAYGMKAHLLNMIVYTLGVLPLTYLI-----------VNLRPKLTVNKNVTTVTMVTTSIDKQCYFITSCMFLVLAM---NTYAVGKEIVQMFQQRLKYLRDMSNLMDWSSTICSLLFVIPLLLNVESSWHWQAGALAGLISWINLLLYLQRFERFGIYVVMFREICRTLLSIIVLFIYLILAFALAFYALMVEQRHFGRMFLSLMQTFVMMVGEISYQDNFLKPYMSSQLPFPLMTYGIFVWFVLLVPILLMNLLIGLAVGDIAEVQTNACLKQIGMQIELHSNLEERLP 971          

HSP 2 Score: 74.3294 bits (181), Expect = 3.980e-13
Identity = 63/241 (26.14%), Postives = 107/241 (44.40%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFL 1202
            L +RD    +  HY   K  +  IK I + I+  Q + +++ +     +PLH+AV       C  LL  GA+PNI   +   PLH+A    H  +V+ L++ S+ +        ++ + +AA   N+E    L   G     +++ G   +  AA +G    +E +L K        E+++N  D+   + LH A   G+L V+Q     GA ++     +S  L  A +      VK  L
Sbjct:   38 LAVRDDTGASPLHYAACKGHLRAIKLIVQ-IAGQQELNVVDEEG---NTPLHWAVQKDQRGSCSALLSLGADPNILNKSLLSPLHLAVSLRHNGLVEQLVSHSQTNVNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVVLQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLML 274          

HSP 3 Score: 66.2402 bits (160), Expect = 1.480e-10
Identity = 75/300 (25.00%), Postives = 127/300 (42.33%), Query Frame = 3
Query:  294 INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIE-------KADL---------NIRDYDMKTIF--------------HYLV-------VKEKIEMIKWIYENISESQRIEL-INSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC----FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQA 1067
            +NV D + N+PLH A+  ++      LL  GA+ N+ NK   +P+ LA   +S   N  G+++ L+         + DL         ++ +++   I               H+ +        K+ +E++  + +       IE  IN  D    SPLH AV  GNL++ Q+ +  GA  N    +    LH A  +G  + VKL+L +  +  C      D    + +H A    +YE+ ++LI  G D    D  G + L+ A   G    +  LL+    + + D+     LH A
Sbjct:   73 LNVVDEEGNTPLHWAVQKDQRGSCSALLSLGADPNILNKSLLSPLHLA---VSLRHN--GLVEQLVSHSQTNVNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVV--LQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRV-CDIINITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLATSCGAWRTVNLLLSHGAELKVRDKAGCNFLHLA 364          

HSP 4 Score: 62.7734 bits (151), Expect = 1.550e-9
Identity = 73/319 (22.88%), Postives = 133/319 (41.69%), Query Frame = 3
Query:  291 NINVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN--INLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV----NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
             + V D+   SPLH+A     L  +K++++      +N+ +++  TP+  A       K+  G    L+   AD NI +  + +  H  V      +++ +   +S SQ    +N +  +  +P+  A S+ N E   +L   GA           P+H AA  G    ++++L   +++         + D + SS +H+A   GN ++ +  I  G     +       L FA   G    ++ +L     V    N+ D    T LH+A    H ++ + L + GA +   +  G SPL +A S      V   L+
Sbjct:   37 QLAVRDDTGASPLHYAACKGHLRAIKLIVQIAGQQELNVVDEEGNTPLHWAVQ-----KDQRGSCSALLSLGADPNILNKSLLSPLHLAVSLRHNGLVEQL---VSHSQTN--VNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVVLQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRVCDIINITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLATSCGAWRTVNLLLS 345          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Match: trpa1a (transient receptor potential cation channel subfamily A member 1-like [Source:NCBI gene;Acc:103022174])

HSP 1 Score: 193.741 bits (491), Expect = 1.422e-50
Identity = 190/791 (24.02%), Postives = 358/791 (45.26%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRI-ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLID------------AGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLI--FLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEF----DLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVP 2345
            +E G+++++ ++  D      L  AV+  NLD+I++C+     IN +Q D  T  H A         KLM     +  DI IN+ D    +PLH A I +  ++ + L+  GA+I+  +    +P+LLA     A +     + LL+   A+L +RD       H  +++ +   +K +   + + + + EL+N +D    +PLHYA  +G  +  + +L    + +  +      LH AAE G ++    LL     +    + +E   + +H+A+  G+ ++ + L+  G      D  G + L  AA  G    +E LL   +N+ L D++     TALH AA  GH+  V+ L   GA    N    S L  A+ N ++ V    ++ D  +  +     N +    + ++I+   +    LLD CI E+  +     Y ++Y F  +                DD   ++    AL +M E+ R++LL HP+  K L++KW  +GM    ++ +++ + ++ +TY ++ N R                              P +T N  +  +  +   I      ++   NL + W++    C+ L +I    P  L     W    G +   +S+   + YLQ     GI ++MF+++ +T++ ++  F  +   FA  FY +  +Q  F  + L+ M++  MM GE     + +  ++S +  + + +  +   FV+++ I L NL+IGLAVGDI ++ + A ++   +++     +E  +P
Sbjct:  204 QEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRVCDI-INITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLA-TSCGAWRT----VNLLLSHGAELKVRDKAGCNFLHLAILQPR--GLKNLPTEVLQHESVRELLNDEDSEGCTPLHYACRLGIPDSVKNMLGLEVSLDQKSKQKKSALHFAAEYGRINTCHRLLEPMTDTRLLNEGDEKGMTPLHLASRGGHVKVVELLLRKGA-LFHSDYKGWSCLHHAAAEGFTQTMETLLI--SNIKLLDKIDGDGNTALHLAARAGHVAAVRQLLCRGAQFVLNKGDASFLHEAVHNGRKEVTSAVIDSDRCEEAITTFKVNSAKRCIVLDMIEFLPESYKHLLDTCIKESDDDVNSCTYNIEYNFQWLQHTNHRIQNTDNIEEDDDYKPLA----ALNAMVEFNRVELLTHPVCKKFLEMKWKAYGMKAHLLNMIVYTLGVLPLTYLIV-NLR------------------------------PKLTVNKNVTTVTMVTTSIDKRLKYLRDMSNLMD-WSSTI--CSLLFVI----PLLLNVESSWHWQAGALAGLISWINLLLYLQRFERFGIYVVMFREICRTLLSIIVLFIYLILAFALAFYALMVEQRHFGRMFLSLMQTFVMMVGEISYQDNFLKPYMSSQLPFPLMTYGIFVWFVLLVPILLMNLLIGLAVGDIAEVQTNACLKQIGMQIELHSNLEERLP 941          

HSP 2 Score: 74.3294 bits (181), Expect = 3.937e-13
Identity = 63/241 (26.14%), Postives = 107/241 (44.40%), Query Frame = 3
Query:  507 LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTK--------ETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQS-PLDIAISNNQENVVKFFL 1202
            L +RD    +  HY   K  +  IK I + I+  Q + +++ +     +PLH+AV       C  LL  GA+PNI   +   PLH+A    H  +V+ L++ S+ +        ++ + +AA   N+E    L   G     +++ G   +  AA +G    +E +L K        E+++N  D+   + LH A   G+L V+Q     GA ++     +S  L  A +      VK  L
Sbjct:   38 LAVRDDTGASPLHYAACKGHLRAIKLIVQ-IAGQQELNVVDEEG---NTPLHWAVQKDQRGSCSALLSLGADPNILNKSLLSPLHLAVSLRHNGLVEQLVSHSQTNVNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVVLQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLML 274          

HSP 3 Score: 65.855 bits (159), Expect = 1.505e-10
Identity = 75/300 (25.00%), Postives = 127/300 (42.33%), Query Frame = 3
Query:  294 INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIE-------KADL---------NIRDYDMKTIF--------------HYLV-------VKEKIEMIKWIYENISESQRIEL-INSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC----FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQA 1067
            +NV D + N+PLH A+  ++      LL  GA+ N+ NK   +P+ LA   +S   N  G+++ L+         + DL         ++ +++   I               H+ +        K+ +E++  + +       IE  IN  D    SPLH AV  GNL++ Q+ +  GA  N    +    LH A  +G  + VKL+L +  +  C      D    + +H A    +YE+ ++LI  G D    D  G + L+ A   G    +  LL+    + + D+     LH A
Sbjct:   73 LNVVDEEGNTPLHWAVQKDQRGSCSALLSLGADPNILNKSLLSPLHLA---VSLRHN--GLVEQLVSHSQTNVNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVV--LQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRV-CDIINITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLATSCGAWRTVNLLLSHGAELKVRDKAGCNFLHLA 364          

HSP 4 Score: 62.7734 bits (151), Expect = 1.460e-9
Identity = 73/319 (22.88%), Postives = 133/319 (41.69%), Query Frame = 3
Query:  291 NINVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN--INLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNV----NLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
             + V D+   SPLH+A     L  +K++++      +N+ +++  TP+  A       K+  G    L+   AD NI +  + +  H  V      +++ +   +S SQ    +N +  +  +P+  A S+ N E   +L   GA           P+H AA  G    ++++L   +++         + D + SS +H+A   GN ++ +  I  G     +       L FA   G    ++ +L     V    N+ D    T LH+A    H ++ + L + GA +   +  G SPL +A S      V   L+
Sbjct:   37 QLAVRDDTGASPLHYAACKGHLRAIKLIVQIAGQQELNVVDEEGNTPLHWAVQ-----KDQRGSCSALLSLGADPNILNKSLLSPLHLAVSLRHNGLVEQL---VSHSQTN--VNLEGDLGNTPVILAASVDNHEALCILQRNGAKLCRQNKLGHFPIHAAAFAGAKKTMEVVLQKGQEAGMSIESHINYVDKSISSPLHLAVRGGNLDVIQLCIAHGAKINQQQCDKSTALHFACTQGATEAVKLMLLAYDRVCDIINITDGACQTPLHKATIFDHYELAEYLISQGADIDFIDCKGHSPLLLATSCGAWRTVNLLLS 345          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 127.487 bits (319), Expect = 2.122e-30
Identity = 101/379 (26.65%), Postives = 175/379 (46.17%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQ---SPLDIAISNNQENVVKFFLN 1205
            +N+  A+D    +G   L  A +  N +++K+ LD    I+TK  DG T  H A+   +  + +++  RD  I     +  SPLH A     L  V++LL   A ++    D+ TP     + ++A      + KLL+    + N R  +  T  H    K +I++++ + ++ +       I++      +P+H A  +G+L + Q+LL+ GA+ N        PLH+A+  G L++VK LL S  +    +   + + +HIA+  G  EI + L+  G D  A    G   L FA+R G   +   LL    +++   +   T LH A+  G L+V   L    A     + GQ   +PL +A   + + V    L+
Sbjct:  225 LNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTP-----LHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGAS------IHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARV-GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLD 591          

HSP 2 Score: 117.472 bits (293), Expect = 2.675e-27
Identity = 103/388 (26.55%), Postives = 184/388 (47.42%), Query Frame = 3
Query:  111 NDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQ-RIELI-------------------NSQDIVDKS---PLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF-GQSPLDIAISNNQENVVKFFL 1202
            +D       A +N NL+ +   L   +DINT   +G    H AS   +  +   +  R+  I+      N+ LH A +  + D+V+VL++FGANIN  +++  TP+ +A  E     N   I+K L+   A+ ++   D  T     + +   +++  + E+ ++ + R+  +                   ++ D+  KS   PLH A   GN+ +  +LL  GA  +  A N   PLH+AA++G+ ++VKLLL+   K +  +  +  + +H AA +G+ ++ + L+      LAK + G + L  A +   L  ++ LL ++  V+      +T LH AA  GH +V + L   G   +     G +PL IA   N+  V++  L
Sbjct:    9 SDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQE-----NHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDT-KTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLL 390          

HSP 3 Score: 112.079 bits (279), Expect = 1.511e-25
Identity = 93/374 (24.87%), Postives = 169/374 (45.19%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLE--AGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNV 1226
            +G   L  A +   + ++++ L     I+T    G T  H AS + +  + +L+     + N  +    +PLH A    +L++VK LL+ GA + +  ++ QTP     + I++    + I++LL+   +D N       T  ++   +   ++   + EN ++   I  +  +     +PLH A   G LE+  +LLE  A A+P +   N   PLH+A    H  +  LLL+    S      N  + +HIAA+    E+ K L+ +G +     + G   L  AA+ G   ++  LL +  +++L  +  +TA+H AA E  + V + L    A + +      +PL +A       +V F L +    N 
Sbjct:  369 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTP-----LHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGAD---ISPVTKKGF---TPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKG-ASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNA 730          

HSP 4 Score: 104.76 bits (260), Expect = 2.623e-23
Identity = 97/373 (26.01%), Postives = 172/373 (46.11%), Query Frame = 3
Query:  105 KDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLL-NSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIG--IGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF---GQSPLDIAISNNQENVVKFFLN 1205
            K  +G   L  A Q ++L  +++ L     ++    D  T  H A+   +  + KL+  R    N    +  +PLH A   N++ ++++LLK GA+I+   +   TPI +A    S + +L+ +++LL+   A  N  +   +T  H      ++E++K + ++ +       +  +   D++PLH A  +G  EI Q+LL+ G++PN   A+   PL+ A+ +GH D+  LLL N +  S   +     + +HIA++ G  E+   L+      D     + G   L  A       +   LL K  + +   +   T LH AA +  L+V + L   GA    NM    G +PL +A      ++V   L 
Sbjct:  300 KTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVA----SFMGHLN-VVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGAR------VGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKG--FTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA--ETNMLTQQGVAPLHLAAQEGHADMVALLLG 657          

HSP 5 Score: 64.6994 bits (156), Expect = 7.117e-11
Identity = 58/230 (25.22%), Postives = 98/230 (42.61%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
            +G   L  A    +  +  + LD     ++   +G T  H A+  N   + K++       N+      +PLH A      DMV +LL   A+I+L  K       L  M ++A ++   + ++L   KAD+N       T  H       I+M+ ++  N +      L+N++     +PLH A   G+  +   LL+  A+PN   AN    L IA   G++ +V  L
Sbjct:  569 NGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGG-----LTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRA------LVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 787          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 124.79 bits (312), Expect = 1.867e-29
Identity = 102/381 (26.77%), Postives = 175/381 (45.93%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLA--CMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL---WTSGAILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            +N+  A+D    +G   L  A +  N +++K+ LD    I+TK  DG T  H A+   +  + +++  RD  I     +  SPLH A     L  V++LL   A ++    D+ TP+ +A  C           + KLL+    + N R  +  T  H    K +I++++ + ++ +       I++      +P+H A  +G+L + Q+LL+ GA+ N        PLH+A+  G L++VK LL S  +    +   + + +HIA+  G  EI + L+  G D  A    G   L FA+R G   +   LL    +++   +   T LH A+  G L+V   L   + S      N  G +PL +A   + + V    L+
Sbjct:  232 LNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYR-------VAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGAS------IHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARV-GVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKN--GLTPLHVATHYDHQKVALLLLD 596          

HSP 2 Score: 113.62 bits (283), Expect = 4.690e-26
Identity = 104/397 (26.20%), Postives = 188/397 (47.36%), Query Frame = 3
Query:  111 NDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINY-PILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQ-RIELI-------------------NSQDIVDK-----------SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF-GQSPLDIAISNNQENVVKFFL 1202
            +D       A +N NL+ +   L   +DINT   +G    H AS   +  ++ +L+K R+  I+      N+ LH A +  + D+V+VL++FGANIN  +++  TP+ +A  E     N   I+K L+   A+ ++   D  T     + +   +++  + E+ ++ + R+  +                   ++ D+  K           +PLH A   GN+ +  +LL  GA  +  A N   PLH+AA++G+ ++VKLLL+   K +  +  +  + +H AA +G+ ++ + L+      LAK + G + L  A +   L  ++ LL ++  V+      +T LH AA  GH +V + L   G   +     G +PL IA   N+  V++  L
Sbjct:    8 SDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLK-REAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQE-----NHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDT-KTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLL 397          

HSP 3 Score: 107.842 bits (268), Expect = 3.654e-24
Identity = 90/366 (24.59%), Postives = 166/366 (45.36%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLND 1208
            +G   L  A +   + ++++ L     I+T    G T  H AS + +  + +L+     + N  +    +PLH A    +L++VK LL+ GA + +  ++ QTP     + I++    + I++LL+   +D N       T  ++   +   ++   + EN ++   I  +  +     +PLH A   G LE+  +LLE  A+ +    N   PLH+A    H  +  LLL+    S      N  + +HIAA+    E+ K L+ +G +     + G   L  AA+ G   ++  LL +  +++L  +  +TA+H AA E  + V + L    A + +      +PL +A       +V F L +
Sbjct:  376 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTP-----LHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGAD---ISPVTKKGF---TPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKG-ASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRN 729          

HSP 4 Score: 107.457 bits (267), Expect = 4.194e-24
Identity = 97/371 (26.15%), Postives = 172/371 (46.36%), Query Frame = 3
Query:  105 KDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLL-NSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF---GQSPLDIAISNNQENVVKFFLN 1205
            K  +G   L  A Q ++L  +++ L     ++    D  T  H A+   +  + KL+  R    N    +  +PLH A   N++ ++++LLK GA+I+   +   TPI +A    S + +L+ +++LL+   A  N  +   +T  H      ++E++K + ++ +       +  +   D++PLH A  +G  EI Q+LL+ G++PN   A+   PL+ A+ +GH D+  LLL N +  S   +     + +HIA++ G  E+   L+        K + G   L  A       +   LL K  + +   +   T LH AA +  L+V + L   GA    NM    G +PL +A      ++V   L 
Sbjct:  307 KTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVA----SFMGHLN-VVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGAR------VGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKG--FTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA--ETNMLTQQGVAPLHLAAQEGHADMVALLLG 662          

HSP 5 Score: 68.5514 bits (166), Expect = 5.196e-12
Identity = 60/235 (25.53%), Postives = 100/235 (42.55%), Query Frame = 3
Query:   99 DEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
            D K  +G   L  A    +  +  + LD     ++   +G T  H A+  N   + K++       N+      +PLH A      DMV +LL   A+I+L  K       L  M ++A ++   + ++L   KAD+N       T  H       I+M+ ++  N +      L+N++     +PLH A   G+  +   LL+  A+PN   AN    L IA   G++ +V  L
Sbjct:  569 DPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGG-----LTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRA------LVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVVDTL 792          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 114.775 bits (286), Expect = 3.023e-26
Identity = 97/400 (24.25%), Postives = 171/400 (42.75%), Query Frame = 3
Query:  105 KDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-------GIGKDCL--------------------------AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFL 1202
            K  +G   L  A Q  +++ +++ + +   ++    D  T  H A+   +    K++  +  N NV   +  +PLH A   N++ ++++LLK+GA+I    +   TP     M +SA      I+ +L+   A  N  +   +T  H      + E+++ +  N ++      ++++   +++PLH A  +G LEI Q+LL+  A+P+   +N   PLH+AA +GH D+  +LL     S         + +H+A++ G  E+   L+         GK+ L                          A  + G   L  AA+   L I   LL      N   +  V  LH  A EGH  V   L    A +      G SPL +A   ++ NV +  +
Sbjct:   35 KTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTP-----MHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQ------VDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHG-ASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 422          

HSP 2 Score: 103.99 bits (258), Expect = 6.815e-23
Identity = 91/370 (24.59%), Postives = 154/370 (41.62%), Query Frame = 3
Query:  153 NLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENIS-------------------ESQRIELI-----NSQDIVDK---SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES-----SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIA 1163
            NL+I+ I L      N     G+T  H A+      + + +      ++    +  +PLH A    KL++V++LL+  A+ +    +  TP     + ++A +    +  +L+   A L +      T  H      K+E+   + +  +                   ++Q++ L+      S     K   +PLH A     LEI   LLE GA  N        PLH+ A++GH D+  LLL        + D N +     S +H+AA+     + + L+  G    A  + G   L+ A   G + ++ +LL    +VN   +   T LHQAA +GH  +V  L   GA  +D  + G + L IA
Sbjct:  150 NLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTP-----LHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR------YADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIA 508          

HSP 3 Score: 69.707 bits (169), Expect = 2.329e-12
Identity = 58/245 (23.67%), Postives = 110/245 (44.90%), Query Frame = 3
Query:  129 LSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLN-SSRKSECFRDSNESSAI 860
            L  A +   +++  + L      +    +G T  H AS  +   +  L+  +  + +    +  +PLH A   ++L++   LL++GA  N   +    P+ L   E  A      +  LL+   AD+N       +  H    ++K+ + + + ++ +       +N+   +  +PL  A   GN+++   LL+ GA+ N    N   PLH AA++GH  IV LLL   +R ++   + N + AI
Sbjct:  274 LHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHA-----DVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAH------VNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAI 507          

HSP 4 Score: 67.3958 bits (163), Expect = 1.181e-11
Identity = 55/211 (26.07%), Postives = 98/211 (46.45%), Query Frame = 3
Query:  651 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES------SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHI 1262
            +PLH A   G+ ++ ++LLE GA       N   PLH+A +  H++ V++L+        +R   +       +A+H+AA  G+Y   K L+  G +   +   G   L  A +   + ++E LL    ++    +  +T +H +A  G+L +V  L   GA   + N+ G++ L +A    Q  VV+  L +    +  A R +    HI
Sbjct:    8 TPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQ-------YRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKA-REEQTPLHI 210          

HSP 5 Score: 65.4698 bits (158), Expect = 4.798e-11
Identity = 53/230 (23.04%), Postives = 95/230 (41.30%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
            +G   L  A +   L+I    L++  + N     G    H  +   +  +  L+ +R  ++N       SPLH A   +K+++ ++L+K GA++N   K   TP+++AC                                  HY      I+M+ ++ ++ ++      +NS+     +PLH A   G+  I  +LL+ GA PN    N    L IA   G++ +V  L
Sbjct:  335 NGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVAC----------------------------------HY----GNIKMVNFLLQHGAD------VNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTL 520          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 2.487e-25
Identity = 98/359 (27.30%), Postives = 163/359 (45.40%), Query Frame = 3
Query:  120 QCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNE--SSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARS--GCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL-WTSGAILSDNMFGQSPLDIAISNNQE 1181
            Q  L  A+ N + D ++  +    ++N    + +T  H A+ +    + +L+      +N  DN   +PLH A  +     V+ LLK  A++N  +K++QTP+ +A     A+K    II +L    +  N+ D   +T  H+       EM+ ++    +       IN+ D  D+  +H+A  +G+ E+ +VL+  GA+          PLH A+  G + +VKLLL+         D N   ++A+H+A  NG   +   LI  G +    +E G   L FAA S  G L  +E L+    +VN+  +   + LH  A  G     Q L    G I  ++  G +PL IA     E
Sbjct:    7 QAPLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAAN-RAVKCAEAIIPML----SSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGAN------INAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVD---IDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGAL-CLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHE 350          

HSP 2 Score: 110.923 bits (276), Expect = 3.855e-25
Identity = 93/350 (26.57%), Postives = 157/350 (44.86%), Query Frame = 3
Query:   75 GINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRK-------------SECFR----DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA 1073
            G N+ NA D+KD      + +A    + +++K+ +    D+  K   G +  H AS      + KL+    ++I+  ++  N+ LH A  N +  +V  L+  GAN+N  N+   TP+  A    +A  + +  ++LL+   AD+N++  D K+  H   V  +    + + +N  E      I+ +D    +PLH A   G+  +   L+  GA+      +   PLH+AA  G  D  + LL++ +K             S  F     D    + +H AA  GN E    L+  G D   KD F R  L +AA +  +  +  L+     +N  D+ + T LH AAA
Sbjct:  161 GANI-NAFDKKDRRA---IHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFA----AASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGE------IDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAA 496          

HSP 3 Score: 88.5817 bits (218), Expect = 3.723e-18
Identity = 98/418 (23.44%), Postives = 170/418 (40.67%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASI-INYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGI-IKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSS----------RKS-----------ECFR---------------DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQV----VQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLNDDNWQ 1220
            +D  D  G+ CL  A    N++ + + L    D+N K    +T  H A+   N   +  L+ S    +N  D    +PLH+A   +           G    +    + T  + + +++ +  + + I ++ L+ + AD  I+D    T  HY         ++ + E     Q     + +     SPLH A   G+    +VL+ +  + ++   N   PL +AA +GH D V++L+             R++           EC R               D+NE + + +A  NG+ +    L+  G    AKD  G   L   A +G    ++ LL +  +V+L ++     LH AAA GH+ +    VQ    SGA +   +  G +PL  A  N  +  V+  L  D +Q
Sbjct:  414 IDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVAS-GARLNEQDERRCTPLHYAAAAD-----------GDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQS----DGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDVFQ 815          

HSP 4 Score: 83.9593 bits (206), Expect = 9.236e-17
Identity = 64/197 (32.49%), Postives = 95/197 (48.22%), Query Frame = 3
Query:  618 ELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            E +N+ D   ++PLH A  +G  EI ++L+ +GA  N        PLH A        V+ LL  S      RD N  + +H+AA N   +  + +I +       D  GR  L  AA +G   ++ +L+ K  N+N  D+    A+H AA  GH +VV+ L   GA +   +  G SPL  A S+ Q  VVK  L+
Sbjct:   30 EEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNA-RDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLD 225          

HSP 5 Score: 81.6481 bits (200), Expect = 4.878e-16
Identity = 70/247 (28.34%), Postives = 122/247 (49.39%), Query Frame = 3
Query:  477 IKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKL---LLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFLN 1205
            ++ LI +K ++N  D + +T  H      + E+I+ +   +S ++    +NS+D    +PLH A +  +    Q LL+  A+ N    N   PLH+AA    +   +    +L+SS  S    D    +A+H AA NG+ E+  FL+  G +  A D+  R  + +AA  G   +++ L+    +V   D+   + LH A++ G + VV+ L   G  + D N FG + L +A  N Q+ VV   ++
Sbjct:   22 VRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLV--LSGAR----VNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVS----DRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELID 258          

HSP 6 Score: 79.7221 bits (195), Expect = 1.710e-15
Identity = 77/330 (23.33%), Postives = 144/330 (43.64%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLD----MVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCL---AKDEFGRNTLMFAARSGCLPIIEYLLTKETNV 1025
            +L+ EN  +   A+D  D + Q  +  AV N + D   + L     IN K   G T  H  +   +     L+  R +++++ +    +PLH A     +     +V+  +  GA I L +++  TP+  AC          G ++LL+ E      ++ +  +  H  V+ +     + + +        ++IN  D   + PLH A    ++E  Q+LL   A  N+       PL +A E G    V++L+NS++     +D+N ++A+H+A    +      ++    D     A +   +  L  AARSG   +++ LL K  ++
Sbjct:  667 RLLVENA-DSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACY-----NGHDGCVELLL-ELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFG----AKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGASI 985          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.768 bits (273), Expect = 1.022e-24
Identity = 95/362 (26.24%), Postives = 165/362 (45.58%), Query Frame = 3
Query:  129 LSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDF-QTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNT-LMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            L  A     +DI+++ L    D+N + + G T    A    Y  + +++     N+   +   ++PL  A     +++ ++LL+ GA IN  + +F ++ + LAC      K    +++ L+   AD   +  +M T      +   +E+ + + E+ ++      +N      +SPL  A   G++E+  +L+E GAN          PL  AA +GH ++V LLL          +  + +A+ +A   G  E+  FLI  G D     E G +T LM AA+ G L +++YLL    NV+       TAL  A   GH  V   L  +GA L  ++  G++PL  A        V+F ++
Sbjct:  171 LMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACY-----KGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQ------VNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADL----ELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLIS 517          

HSP 2 Score: 101.293 bits (251), Expect = 5.118e-22
Identity = 98/392 (25.00%), Postives = 169/392 (43.11%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDL-NSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPN--------------------------IFAAND-----CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTA------LHQAAAEGHLQVVQCLWTSGA 1118
            +L+  +G +V    + + + G   L+YA     +D++++ L+   ++      G T    A+   +  + +L+  R   IN   N+   S L  A     L+MV+ LL+ GA+      +  T ++ ACM+         + +LL+   A +N+     ++          +E+   + E       +E +N +     +PL  A   G+ E+  +LL  GAN N                          I A  D       PL  AA++GHL++VK LL +         + ++ A+  A ENG+ ++   L+  G D   + E GR  LM AAR+G +  +++L++K  +VN     + TA      L  A A GHL VV+ L   GA
Sbjct:  184 RLLLAHGADV----NAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMD-----GHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIER---GANLEEVNDEGY---TPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLIKAGADLELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDT-ALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVN-----RATANNDHTVLSLACAGGHLSVVELLLAHGA 554          

HSP 3 Score: 78.1814 bits (191), Expect = 5.442e-15
Identity = 78/302 (25.83%), Postives = 137/302 (45.36%), Query Frame = 3
Query:  318 NSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLL-IIEKADLNIRDYDMKTIFHYLVVKEK---IEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNE--SSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIAISNNQENVVKFFL 1202
            N  L  A  +  ++ V+ LL  G +++   ++ ++ + LAC         +G  +L  ++     N+ D  +K     L+       +++++ +  + ++      +N+Q     + L YA + G +++ +VLLEAGAN      +   PL  AA  GH+++ +LLL   R +     SNE   SA+ +A   G+ E+ +FL+  G D   K +     LM A   G + +   LL     VN+      + L  AA  GH+++   L   GA L + N  G +PL  A     E +V   L
Sbjct:  101 NRSLAEACSDGDVNAVRKLLGEGRSVHETTEEGESLLCLACS--------AGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGAD------VNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLE--RGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLL 386          

HSP 4 Score: 71.2478 bits (173), Expect = 9.029e-13
Identity = 83/343 (24.20%), Postives = 149/343 (43.44%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGK-TIFHTASIINYPILFKLMKSRDIN---INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANIN--LCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIR-DYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL 1103
            ++ +D  G   L  A    +++++++ LD   DI  +    K T    A       + +L+ +R  N    NV D    +PL  A     ++++K+LL  GA IN    +K   +P++LA M        +  +KLL+   +D+N + + +  T       + + E++  + E      R   +  +     +PL  A S G  E+ +VLLE GA                      D+    + SSR           +A+ IAA+ G+Y+  + +I  G     +++ G   L  AA  G L +++ L+    +V+  D  K+T L  A  +GH++VV+ L
Sbjct:  959 IEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEHRNVSDY---TPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAM-----NGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVE------RKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGA----------------------DVNAAPVPSSR----------DTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYL 1255          

HSP 5 Score: 69.3218 bits (168), Expect = 2.943e-12
Identity = 83/326 (25.46%), Postives = 136/326 (41.72%), Query Frame = 3
Query:  357 DMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMK-TIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAAN--DCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVN-----------------------------------LHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL--SDNMFGQSPLDIAISNNQENVVKFFLNDDN 1214
            D+V VL+  GANI   +K   TP++LA     A      +++LL+   AD+  +    K T         + E+++ +    +  +       +++ D +PL  A S G + I ++LL AGA  N    +     PL +AA  GH   VKLLL+         ++N ++A+ +A   G +E+   L+    +   + + G   LM AA  G   +   LL +  +VN                                   + ++   T L  AA  GHL VVQ L  SGA +  +DN    +PL  A       VV++ + + N
Sbjct:  947 DLVTVLIARGANIEHRDKKGFTPLILA-----ATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEH------RNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRK-ITPLMAAFRKGHVKVVRYLVKEVN 1260          

HSP 6 Score: 67.781 bits (164), Expect = 1.071e-11
Identity = 65/304 (21.38%), Postives = 132/304 (43.42%), Query Frame = 3
Query:   99 DEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQ-TPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL 1007
            + K ++    L  A  + ++++ ++ L+    +N      ++    A+   +  L  L+  R  N+   +++  +PL  A      +MV +LL  GANIN   ++ Q T + LAC           +   LI   ADL +      T       +  +E++K++    +       +++      + L YA   G+ ++  +LL+AGA+    +     PL  AA  GH+  V+ L++          +N+ + + +A   G+  + + L+  G D   + + G   L+ AA+ G   ++ YL+
Sbjct:  294 EHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLAC-----CGGFLEVADFLIKAGADLEL---GCSTPLMEAAQEGHLELVKYLLAAGAN------VHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLLAHGADPNHRLKDGSTMLIEAAKGGHTSVVCYLI 583          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 75.8702 bits (185), Expect = 6.657e-16
Identity = 67/207 (32.37%), Postives = 100/207 (48.31%), Query Frame = 3
Query:  612 RIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAAND---CLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES-SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLT-KETNVNLHDQMKVTALHQAAAEGHLQVVQCL---WTSGAILSDNMFGQSPLDIAISNNQENVVKFFLND 1208
            +  L+  +D   + PLH++VS    EI   LL    N N+    D     P HIA   G+L++VK L +   K +  + +N+  + +H+A     +E+ +FLI  G     KD+F +  L  AA  G L +IE L    ++ VN  D+   T L  A AEGH      L   + +   L DN  G    D+A++   E V KFFLN+
Sbjct:   25 KPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNK-GAKAEDVALN---EQVKKFFLNN 227          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672])

HSP 1 Score: 58.151 bits (139), Expect = 3.178e-9
Identity = 44/136 (32.35%), Postives = 61/136 (44.85%), Query Frame = 3
Query:  651 SPLHYAVSIGNLEICQVLLEAGANPN----IFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMK 1046
            S LHYA   G   IC  L++ G + +     F  N C+  H+A  KGH   + LLL    +    R  N  + IHIA  N  Y+    LIG+G D    D  G   L      G +  ++ LL  E  V+L D ++
Sbjct:   42 SSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCV--HLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLN-EGEVSLDDNVR 174          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 56.9954 bits (136), Expect = 1.289e-8
Identity = 40/126 (31.75%), Postives = 64/126 (50.79%), Query Frame = 3
Query:  837 DSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKET-NVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA---ILSDNMFGQSPLDIAISNNQENVVKFFL 1202
            DSN+SS I ++                K  + +D  GR  L  A   G   +++ ++ +   ++N  D    TALH+AA +GH+++V+ L  +GA   I S  MFG +PL  A +N   +VVK+ L
Sbjct:  305 DSNKSSHIAVSKRPKQ-----------KKGIYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLL 419          

HSP 2 Score: 50.8322 bits (120), Expect = 9.267e-7
Identity = 35/104 (33.65%), Postives = 53/104 (50.96%), Query Frame = 3
Query:  816 RKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGK-DCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLH--DQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            +K   +RDS   + + IA + G Y++ K +I  G  D   +D  G   L  AA  G + I+E L+    +VN+   +    T L  A+A GHL VV+ L  +GA
Sbjct:  320 QKKGIYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGA 423          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560])

HSP 1 Score: 53.9138 bits (128), Expect = 8.485e-8
Identity = 53/185 (28.65%), Postives = 82/185 (44.32%), Query Frame = 3
Query:  585 IYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAA-NDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGK------DCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            + +++ ES  ++ IN+  I + S LH+A       + + LL  GANPN  A       LH AA  G++ IV LLL         +D    + +H +  + N  +  +++          D  +KD   R  L++AA  G    +E LL   + V   D     ALH A   G  +V+  L  SGA
Sbjct:   65 VVKDVVESGAVD-INNDRIDELSGLHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHG-ADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGA 247          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 48.9062 bits (115), Expect = 3.457e-6
Identity = 57/230 (24.78%), Postives = 86/230 (37.39%), Query Frame = 3
Query:  618 ELINSQDIVDKSPLHYAVSIGNLEICQVLL--------------------------------------EAGANP----------NIFAANDCL--PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESS----------------AIHIAAE-NGNYEIFKFLIGIGKDCLAKDEFGRNTLMF-AARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL 1103
            +L+N+QDI  + PLHYA  +G LE    LL                                       A A+P          N+ ++      PL++A +  + D  KLLL    K       N+SS                 +HI A+  G+ ++ + LI  G D    D F + T +F A RSG   +I  LL     +++ D    + L  A  E H+ V+  L
Sbjct:  415 KLLNAQDIHSRVPLHYAAELGKLEFVHSLLITNLLEDVDPIDSDSKTPLVLAITNNHIDVVRDLLTIGGANASPIEKPILDYSKNVISSTKVQFDPLNVACKFNNHDAAKLLLEIRSKQNADNAKNKSSQHLCQPLFKKNSTGLCTLHIVAKIGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNAL 644          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO39112 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB40])

HSP 1 Score: 125.946 bits (315), Expect = 7.364e-30
Identity = 90/291 (30.93%), Postives = 148/291 (50.86%), Query Frame = 3
Query:  531 KTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAV-SIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD 1397
            +T  H          ++ I  N  +S    +INS+D   K+PLHYAV      +I Q+L+E+GAN      ++ LPLH AAE+G LD + +LL ++ ++    D+   +A+HIAA     E  + L+  G +   +D+     L + A  GC   ++ LL  + ++   D+ + T L  AA  G L+V + L  SGA I ++   G + LD+A+ NN  ++    +  ++W+ ++     +G S ++ LI+    L   +LDKCI  +T    D  Y V Y F L+   PD
Sbjct:    4 RTCLHVAAACSDFRTVEIILRNGGKS----IINSKDSYGKTPLHYAVIRCQGGKIIQLLIESGANVLAVDFDERLPLHHAAEQGMLDNISILLQANPETINEVDNRSLTALHIAAAAARTEACRLLLEKGAEVDYRDDERLTPLFYVASVGCPLTVKVLLEYDADLEARDKNRKTPLISAAYFGRLEVFRTLLDSGADIAAETSSGWNALDVALDNNNLDITNEIIRHESWKTLLLNTKADGVSPLKKLIERVPCLAKVVLDKCIEHSTWVDTDKEYSVTYDFQLLSPPPD 290          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO27354 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T9H1])

HSP 1 Score: 117.087 bits (292), Expect = 2.122e-27
Identity = 122/514 (23.74%), Postives = 224/514 (43.58%), Query Frame = 3
Query:  627 NSQDIVDKSPLHYAVSIGNLEICQVLLEAGAN---PNIFAANDCLPLHIAAE-KGHLDIVKLLLNSSRKSEC-FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVT-SNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIIS-----NPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD 2135
            +++   + +P   AV     +    LL+ GA+    N+F  +    LH+A       + +KLLL+    S    RD +  + +H+AA  G+ E    L+G  K+C    +   +   F           Y++  E N+                     V  CL     +   N   ++ LD+AI   +  V K  +  D W  V+  R+  G   +  LI++  D+    LD+CI+         +Y + +   L+D GP      S    A + M E    +LL HP++  LL+ KW  FG V + I  ++FL+++IL +YF+++ ER  DS ++   T    +    Q  EK+ +S  ++T   IL   I  ++    +  + +  F+++        + C   A+I IS      P        W      +FL+Y   + Y++ + S G+ + M+ +V  + ++V+  F  +  G    +Y++ +++D F  +  A++++  MM GE D
Sbjct:   31 DTRGFENNTPFLLAVKYDKTQAAAELLKRGADIKAKNLFFLSS---LHLAVSLHKDCETLKLLLDWDDGSLLNARDLDLQTPLHVAAARGSPESINLLLG--KNCTIDAKDANDQYPF-----------YIV--EVNI---------------------VKLCLAEGADVTXRNKDDKTCLDVAIDWKRTEVAKVLIQHDRWWEVICTRNSAGELPMVGLIEALPDVAKLALDQCIVRCPLPTSHQDYCLTFHTVLLDPGPCKEGQTSR--DASEVMVENDCEELLSHPVTQGLLRYKWFAFGKVPYYIYLMVFLIFVILFSYFIIT-ER--DSSTLYHPTHPNASALEKQYLEKRPSSTNSITIVLILFATIEFLKEFYQIFMIRLDYFKDITNLVELVLYTC---AIIFISPYLAEEPLYTAAYGHWNAGVVTLFLAYLNVLLYIKGIGSSGLYVTMYFEVWYSFLKVITVFLVVLLGSTLVYYLLLKEEDDFSGVHYAFVRTFVMMVGEID 497          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO33180 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST04])

HSP 1 Score: 100.908 bits (250), Expect = 5.315e-22
Identity = 91/358 (25.42%), Postives = 161/358 (44.97%), Query Frame = 3
Query:  936 DEFGRNTLMFAARSGCLPIIEYLLTKETN-VNLHDQM-KVTALHQAAAEGHLQVVQCLWTS-GAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPD----DPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYF-VLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEF--WTNCYFACNSLALIIISNPSKLPCLYEWEKVP---GLIFLSYFLFIYYLQHLPSVGIQLIM 1970
            D  GR  L FAA  G L  ++ +     N +N  D   + T+LH AA  GH +VV  L +     ++ N + Q+ LD+AI   +ENVV    N   W+  M +R + G + +++L+        + LD+C+ E  + K D +Y V + F  +   PD    DP    +  H   +M +YKR+  L H +   ++ IKW   G   + ++   +L++++L+T   ++ + RI D+  +D+                     P  T    II++     +  +  +  +KF+ L +   W   +   +SL  +I             E  P     IF+++ +F+ YL+   + GI +IM
Sbjct:   15 DTEGRTALHFAAAKGSLRSVQLICMANPNCINDEDNADQDTSLHLAAKNGHSEVVDFLLSDPNQKITYNGYNQNGLDVAIEAGRENVVMVIANHTRWRE-MCHRVEEGLTQLQHLVIKMPSAAEKFLDRCVEECGAGK-DEDYSVTFDFMFLQGMPDIRKDDPKKFLDCLH---TMVKYKRVNCLSHQVCGAIMNIKWRSVGWKAYVMNLTFYLIFLLLLTSMTIMLDARIEDNLVIDI---------------------PKYT----IIVMSLFHLLKELFQIWDEKFKYLLKADNWMEWFLYMSSLVYMIQYEVFDNHVSVTGETRPLAATAIFIAWVIFVLYLRRFSTFGIYIIM 342          

HSP 2 Score: 50.447 bits (119), Expect = 3.109e-6
Identity = 31/112 (27.68%), Postives = 58/112 (51.79%), Query Frame = 3
Query: 2076 FKNLGLAYMKSLAMMTGE--FDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHK 2405
            F  L  +   +  ++ GE  +    Y      N +++ +I I +F V M I + NL+IGL+VGD+  I   A M+ + ++V  ++E+E   P  + R ++ +   D P ++K
Sbjct:  419 FDELPYSLFTTFMLILGEMKYPHTVYKYQPIPNPWISYIIYI-IFCVCMPIIIKNLLIGLSVGDVNRILQTAKMEQHAMQVELLLELERATPRRVLRKIWVAYFVDFPNRNK 529          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 89.3521 bits (220), Expect = 1.424e-18
Identity = 107/425 (25.18%), Postives = 191/425 (44.94%), Query Frame = 3
Query:   81 NVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDIN-INVGDNDLNSPLHHAIINNKLDMVKVL---LKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDY-DMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEI------CQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA--ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNG---SSHIENLIKSNEDLTLELL 1307
             V   + ++D + Q  L  AV+  + D+ K+CL+   ++N  +      FH   ++    +F ++K   +  ++   ++    +   I+    D+V ++   ++F A I   +KD  TP++     I+A KN    ++ L+   AD+  +D  D  ++F     +   E      E   E Q+        I D   L+  V   N  I       Q+LL+ GA  +     D  P+H AA+ G +  V  LL          D   ++ +H+AA +G+  + K L+  G    A++      L  AA  G + +   LL ++  V+  D+ K T LH A+ EGH+++V+ L +  A   L+D+  G++ LDIAI ++ + V    ++  +W+  M  R   G   +S +  LIK      L LL
Sbjct:   68 TVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSE---KIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLM-----IAAEKNHVDTVRTLLQHGADIAAKDINDRNSVF-----RAAAEGCMETLEAALEHQK----PVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSA-GRNCLDIAIESSHKEVAVAIIDHVDWKQAMRNRCKLGNAITSPMRKLIKKLPGQKLYLL 474          

HSP 2 Score: 51.6026 bits (122), Expect = 1.165e-6
Identity = 30/98 (30.61%), Postives = 49/98 (50.00%), Query Frame = 3
Query:  651 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSR---------KSECFRDSNESSAIHIAAENGNYEIFKFLIGIG 917
            +PLH A S G + + + L+EAGA+          PLH A  +G+L + KLL  ++          K    +D  E +A+H+A E G+ ++ K  +  G
Sbjct:    6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKG 103          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84])

HSP 1 Score: 78.1814 bits (191), Expect = 3.575e-16
Identity = 65/240 (27.08%), Postives = 102/240 (42.50%), Query Frame = 3
Query:  423 PILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLD---IVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGR---NTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL 1124
            P+ LAC     L   +   K L+    D+N +D + +T  H       +  ++ +     +       N  D + ++PLHYA    N      L+ AG+N N      C PLH AA    LD   +  LL N    S+  RD    SA+H A+ NG+    + L+ +  + L     G      L  AA  G  P +E L+    ++ + D    T L  A+ +GH++ V+CL    A+ 
Sbjct:    3 PLHLAC-----LGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGD------FNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASD-LDAKCVEHLLRNRGNASK--RDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNAMF 228          

HSP 2 Score: 54.299 bits (129), Expect = 4.027e-8
Identity = 51/231 (22.08%), Postives = 106/231 (45.89%), Query Frame = 3
Query:  228 FHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKE-KIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANP--NIFAANDCL-PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI 908
             H A +  +    K +    +++N  DN+  +PLH A  +  ++ +++L+    + N  +K  + P+  A    +   N   ++  L+   +++N  D    T  HY    +   + ++ +  N   + +      +D    S +HYA + G+    ++LL+  AN   N  + + C+ PLH+AA  GH   +++L+ S    E  RD    + + +A+  G+ E  + L+
Sbjct:    4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYA----AGHTNFRCVMS-LVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASK------RDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLE-VRDDRGRTPLDLASFQGHVECVECLL 222          

HSP 3 Score: 52.373 bits (124), Expect = 1.838e-7
Identity = 55/222 (24.77%), Postives = 100/222 (45.05%), Query Frame = 3
Query:  159 DIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSG-IIKLLIIEKADLNIRDYDMKTIFHYLVV---KEKIEMIKWIYENISESQRIELIN-SQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLN 809
            +  K  L + VD+N K  + +T  H A+        +L+ S+  + N  D    +PLH+A  +     V  L+  G+N+N  +    TP     +  +A  +L    ++ L+  + + + RD    +  HY      K  IEM+  +  N       +L+N S      +PLH A   G+    +VL+++  +  +       PL +A+ +GH++ V+ LLN
Sbjct:   14 ECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTP-----LHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSAN-------DLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLN 223          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: trpa1b (transient receptor potential cation channel subfamily A member 1 [Source:NCBI gene;Acc:101174541])

HSP 1 Score: 206.838 bits (525), Expect = 9.535e-55
Identity = 209/847 (24.68%), Postives = 402/847 (47.46%), Query Frame = 3
Query:   57 KLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSR----DININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIE-LINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIF--AANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNES--SAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKV---TALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSH--IENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISER------YHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINN----SSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEF------WTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFL-FIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFD----LVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSFIFTKSEFMFNALIWSR 2492
            K  +E+G      ++  D      L  AV+  N++ I++C+     I+ +Q D  T  H A       + KLM S     +  IN+ D    +PLH A I +  ++ + L+  GA++N C+    TP+LLA     A K     + LL+ + A++N++D       H  + + K   +K I E + +   ++ L++ +D    +PLHYA  +G  +  + +L       +   + +    LH AA+ G ++  + LL +   S    + +E   + +H+A++ G+ ++ + L+  G      D  G + L  AA  G    +  LL+   N+ L D+      TALH AA  GH+  V+ +   GA L  N    S L  A+ N +++VV   ++ D  +  +       S    I ++I+   +    LLD C+ E+  +   P+Y ++Y F  + A P  P  +S+         A+ +M +Y R++LL HP+  K L +KW  +G     ++  L+L+ ++ +T+ ++S    +++     +TG  + N    S T+ +  KS S       +++++I+ I +I   +  + Q+  N F        W +  ++   +  ++I+    L     W+    L  L Y++ F+ YLQ   +VGI ++MF ++++T++R++  FF +   F+  F+ +  +Q  F+ + L+ +++  MM GE +    ++  F+  +  + + +  +   FV++M I L NL+IGLAVGDI ++   A+++   +++     +E  +P    + +    I   P +   R  ++ LF       K+E      +WSR
Sbjct:  216 KAGEEHGHPAAVHINYLDKSNSSPLHLAVRGGNIEAIRLCIATGAKIDQQQNDRSTPLHLACTQGATEVVKLMLSTVDQVEDFINLTDGACQTPLHRATIFDHSELAEYLISLGADLNSCDCKGNTPLLLA-TSCGAWK----CVALLLSKGANVNLKDKCGCNFLHLAIHQPK--GLKNIPEEVLQRNSVKALLSCEDNEGCTPLHYACRLGIHDSVKNMLGLSGQLGLACKSKDKKSALHFAAQYGRINTCQRLLETITDSRLLNEGDERGLTPLHLASKEGHTKVVQLLLRKGA-LFHSDYKGWSCLHHAASEGYTQTMAILLS--ANLKLLDKTDEDGNTALHIAARAGHVAAVRLMLVKGAELVLNKNHTSFLHEAVQNGRKDVVNAVVDSDRCEEALQLFTPGTSQRCPILDMIEFLPETYKHLLDCCVRESDDDHNSPDYHIEYNFQWLQA-PISPQKLSDESLKIQPLVAVNAMVKYNRIELLNHPVCKKYLAMKWVAYGSKAHALNMFLYLLGLLPLTHLIVSLRPSMNT----TDTGDHVINMVPVSFTEQTVFKSFS-------MVMVLIMNIYSIGKEIVQLSQQRWNYFRDHSNHNDWASAVYSLLFIIPLMINAEGSL----HWQ-AGALAVLHYWIGFLLYLQRFENVGIFVVMFGEIMRTLVRIVMLFFFLMLAFSLAFHALMLNQSQFETVPLSVIQTFVMMVGELNYQNNILDAFLKNELPFGILTYFIFVQFVMLMPILLVNLMIGLAVGDIAEVQRNASLKRIAMQIDLHTSLEDKLPYWFMKRVDKPSIIIYPNRKCSRHFLTHLFSGE--EEKNE------VWSR 1027          

HSP 2 Score: 93.9745 bits (232), Expect = 3.661e-19
Identity = 89/332 (26.81%), Postives = 149/332 (44.88%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFC--VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA-NINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIEL--INSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECF---RDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQA 1067
            L EKD  G   L +A    ++ +I+  +      ++N     G    H A   N P   + +     + NV +  L SPLH A+   + D++K+LL + A + NL      TP+++AC    ++ N   +  +L+   A L  ++       H        + ++ I +   E        IN  D  + SPLH AV  GN+E  ++ +  GA  +    +   PLH+A  +G  ++VKL+L++  + E F    D    + +H A    + E+ ++LI +G D  + D  G   L+ A   G    +  LL+K  NVNL D+     LH A
Sbjct:   53 LSEKDECGASPLHHAAAGGHVTLIQFIITVLDPKELNCSDDQGNVPLHWAVERNKPESCRALLDLGADPNVLNTALLSPLHLAVSLRQNDLMKLLLSYNATDCNLQGDLGNTPLMMAC----SINNCEALT-ILLKHGAKLCQQNKLGHFPMHAAAFAGAKKAMEVILKAGEEHGHPAAVHINYLDKSNSSPLHLAVRGGNIEAIRLCIATGAKIDQQQNDRSTPLHLACTQGATEVVKLMLSTVDQVEDFINLTDGACQTPLHRATIFDHSELAEYLISLGADLNSCDCKGNTPLLLATSCGAWKCVALLLSKGANVNLKDKCGCNFLHLA 379          

HSP 3 Score: 64.3142 bits (155), Expect = 5.773e-10
Identity = 61/271 (22.51%), Postives = 117/271 (43.17%), Query Frame = 3
Query:  420 TPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL--------TKETNVNLHDQMKVTALHQAAAEGHLQVVQ-CLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            +P  L  +E++   +L+ +  L+      L+ +D    +  H+      + +I++I   +   +    +N  D     PLH+AV     E C+ LL+ GA+PN+       PLH+A      D++KLLL+ +      +    ++ + +A    N E    L+  G     +++ G   +  AA +G    +E +L            ++N  D+   + LH A   G+++ ++ C+ T   I        +PL +A +     VVK  L+
Sbjct:   24 SPASLNAVELAEKGDLAHLENLVRKSPEVLSEKDECGASPLHHAAAGGHVTLIQFIITVLDPKE----LNCSDDQGNVPLHWAVERNKPESCRALLDLGADPNVLNTALLSPLHLAVSLRQNDLMKLLLSYNATDCNLQGDLGNTPLMMACSINNCEALTILLKHGAKLCQQNKLGHFPMHAAAFAGAKKAMEVILKAGEEHGHPAAVHINYLDKSNSSPLHLAVRGGNIEAIRLCIATGAKIDQQQNDRSTPLHLACTQGATEVVKLMLS 290          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 138.658 bits (348), Expect = 6.525e-33
Identity = 101/365 (27.67%), Postives = 172/365 (47.12%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            +G   L  A +  +L ++ + L     I      G T  H AS + +  + K++  +  + +  +  + +PLH A  +   ++ + LL+  A ++   KD QTP  L C      K L   +KLL+  KA+ N       +  H    +  ++ ++ + +   E+Q+ ++         +PLH A   G +++ ++LLE GANPN    N   PLH+A    +LD+V LL+ S   S      N  +A+HIAA+    E+   L+  G    A+   G   L  A++ G   I+  L++K+ NVNL ++  +T LH  A EGH+ +   L   GA + +    G +PL +A       +VKF L 
Sbjct:  308 NGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTP--LHCAARMGHKEL---VKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLD--MEAQQTKMTKK----GFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLV-SKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQ 660          

HSP 2 Score: 104.375 bits (259), Expect = 2.643e-22
Identity = 107/399 (26.82%), Postives = 170/399 (42.61%), Query Frame = 3
Query:   78 INVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLN-----------SPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAIL-SDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYR 1238
            +N    ++ +   G   L  A Q  +L+++K  L+   + +    DG T    A    +  +  L+ S      V    L            +PLH A     L++ ++LL  GAN  L      TP  L C   +A      II++L+   A +  +  +  +  H     + ++ +K + +  +E   I L +       +PLH A   G+  + +VLL+ GA PN  A N   PLHIA +K HL ++ LLL  S   E   +S   + +H+A+  G+  I K L+  G    A +      L  A+RSG   + E+LL     V+   +   T LH AA  GH ++V+ L    A   S    G SPL IA        V+  L+ +  Q  M  +
Sbjct:   93 VNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDEL------TP--LHC---AARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDH------LTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESG-LTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKK 473          

HSP 3 Score: 103.99 bits (258), Expect = 3.413e-22
Identity = 97/382 (25.39%), Postives = 177/382 (46.34%), Query Frame = 3
Query:   69 ENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEM-IKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLND 1208
            +NGI++  A    + +G   L  A +  ++ ++   L   + + T    G T  H A++     +   + +   N+N       +PL+ A   N L++VK LL+ GAN ++  +D  TP+ +A  +         ++ LLI       +R   +     +L V     + I   YEN++ +Q   L+N     + +PLH A   G+  I ++LL+ GA       N   P+H+AA+  H+D VK LL  + + +     +  + +H+AA  G++ + K L+  G    ++   G   L  A +   L +++ LL    ++    +  +T LH A+  GHL +V+ L   GA  S  N+  ++PL +A  +    V +F L +
Sbjct:   28 KNGIDINTA----NQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQ-----GHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQL--LLNRGANDELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDI-TLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQN 397          

HSP 4 Score: 56.6102 bits (135), Expect = 1.136e-7
Identity = 54/192 (28.12%), Postives = 88/192 (45.83%), Query Frame = 3
Query:  666 AVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG-------AILSDNMF-----GQSPLDIAISNNQENVVKFFLN 1205
            A   GNL+     ++ G + N    N    LH+A+++GH+ +V  LL++    E       ++A+HIAA  G  ++   L+  G +  A+ + G   L  AA+   L ++++LL    N ++  +   T L  A  +GH  VV  L + G         L+D  F     G +PL IA      NV +  LN
Sbjct:   14 AARSGNLDKALEHIKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKG-NTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLN 204          

HSP 5 Score: 51.2174 bits (121), Expect = 5.933e-6
Identity = 32/133 (24.06%), Postives = 62/133 (46.62%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLN 512
            +G   L  A +   +++    L      N +   G T  H AS    P +  L+ S+  N+N+G+    +PLH       + +  +L+K GA++    +   TP+ +AC       N+  ++K L+ ++A++N
Sbjct:  539 NGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACH----YGNIK-MVKFLLQQQANVN 666          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 131.339 bits (329), Expect = 1.445e-30
Identity = 102/378 (26.98%), Postives = 173/378 (45.77%), Query Frame = 3
Query:  114 DGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDD---NWQNVMAY 1235
            +G   L  A +  ++  + + L     +      G T  H A+ + +  + K +  R  + N  +  + +PLH A      ++ + LL+  A ++   KD QTP  L C      K L   +KLL+  KA+ +       T  H    +  I  I+ + +  + +Q++++         +PLH A   G +++ ++LLE GANPN    N   PLH+A    +LD+VKLL+ S   S      N  + +HIAA+    E+   L+  G     +   G   L  A++ G   ++  L++K+ NVNL ++  +T LH  A EGH+ +   L   GA + + +  G +PL +A       +VKF L      N +  M Y
Sbjct:  368 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTP--LHCAARMGHKEL---VKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLD--AGAQQVKMTKK----GFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLV-SKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGY 733          

HSP 2 Score: 122.479 bits (306), Expect = 7.662e-28
Identity = 88/350 (25.14%), Postives = 163/350 (46.57%), Query Frame = 3
Query:  120 QCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGAN-INLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSD-NMFGQSPLDIA 1163
            +  L  A +  + ++ +  L     ++ +  D +T  H A+ + +  L KL+     N +      ++PLH A     +  +++LL  GA  + +  K F TP     + +++      + +LL+   A+ N    +  T  H  V    ++++K +      +      +S      +PLH A     +E+  VLL+ GA+PN  +     PLH+A+++G  D+V +L+ S + +    + N  + +H+ A+ G+  I   L+  G    A    G   L  A   G + ++++LL ++ +VN   +M  T LHQAA +GH  +V  L   GA+ ++    G SPL IA
Sbjct:  436 ETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGF-TP-----LHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSA------HSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLI-SKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIA 772          

HSP 3 Score: 116.316 bits (290), Expect = 6.628e-26
Identity = 104/425 (24.47%), Postives = 176/425 (41.41%), Query Frame = 3
Query:  117 GQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLAC----------------------------MEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWI------YENISES---------------------QRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWT-SGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQN 1223
            G   L  A   +N+ + ++ L+   ++N    +G T  H AS     ++ +L+  R   I+    D  +PLH A  N  + ++++LL+ GA I    K+  +PI +A                             + ++A      + K+L+ + A  N R  +  T  H    K  +  +  +       E ++ES                     QR    N+ ++  ++PLH A   G+ E+ Q LL+  A  +  A +D  PLH AA  GH ++VKLLL+     +    +  +  +HIAA  G+    + L+  G   +   + G   L  A++ G + + E LL +  N N   +  +T LH A    +L VV+ L +  G+  S    G +PL IA   NQ  V    L +    N
Sbjct:  204 GFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTP-LHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPN 627          

HSP 4 Score: 104.76 bits (260), Expect = 2.391e-22
Identity = 99/372 (26.61%), Postives = 164/372 (44.09%), Query Frame = 3
Query:  126 CLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYR 1238
             L  A +N +     + L    + +     G T  H A+      + +L+ +R  N+N    +  +PLH A     + MV++LL  GA I+   KD  TP  L C   +A      II++L+   A +  +  +  +  H     + ++ ++ + +  +E   I L +       +PLH A   G+  + +VLL+ GA  N  A N   PLHIA +K H+  + LLL  S   E   +S   + +H+AA  G+  I K L+  G    A +      L  A+R+G   + ++LL     V+   +   T LH AA  GH ++V+ L    A   S    G +PL IA      + ++  L+    Q  M  +
Sbjct:  174 ALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTP--LHC---AARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDH------LTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESG-LTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKK 533          

HSP 5 Score: 75.485 bits (184), Expect = 2.165e-13
Identity = 79/326 (24.23%), Postives = 139/326 (42.64%), Query Frame = 3
Query:  336 AIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKL---LIIEKADLNIRDYDMKTIFH--YLVVKEKI--EMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSE---------CFRDSNESSAI-------------------HIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            A  +  LD     +K G NIN+ N++    + LA  E        G +K+   L+    +L        T  H   L  +EK+  E++ +             +N+Q     SPL+ A    +LE+ + LLE GAN ++   +   PL +A ++GH ++V LL+N   K +            D   ++A+                   HIAA   N  + + L+  G +     + G   L  A+R G + ++  LL +   ++   + ++T LH AA  GH+++++ L   GA I +    G SP+ +A   +  + V+  L 
Sbjct:   17 AARSGNLDKALDHIKNGININIANQNGLNGLHLASKE--------GHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGAN----------VNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQ 324          

HSP 6 Score: 69.3218 bits (168), Expect = 1.833e-11
Identity = 51/231 (22.08%), Postives = 97/231 (41.99%), Query Frame = 3
Query:  111 NDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLL 803
             +G   L  A +   +++  + L      N +   G T  H AS    P +  ++ S+  N+N+G+ +  +PLH       + +   L+K GA++   ++   TP+ +AC                                  HY      I+M+K++ +  +       +N++  +  +PLH A   G+ +I  +LL+ GA PN   +N   PL IA   G++ ++ +L
Sbjct:  598 RNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVAC----------------------------------HY----GNIKMVKFLLQQQAH------VNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVL 784          

HSP 7 Score: 58.9214 bits (141), Expect = 2.714e-8
Identity = 53/189 (28.04%), Postives = 87/189 (46.03%), Query Frame = 3
Query:  666 AVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMA 1232
            A   GNL+     ++ G N NI   N    LH+A+++GH+ +V  LL+S  + E       ++A+HIAA  G  ++   L+  G +  A+   G + L  AA+   L ++++LL    N +L  +   T L  A  +GH  VV  L   G   +        L IA  N+        L +D   +V++
Sbjct:   17 AARSGNLDKALDHIKNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKG-NTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLINYG---TKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLS 201          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ankrd44 (ankyrin repeat domain 44 [Source:NCBI gene;Acc:101173198])

HSP 1 Score: 119.013 bits (297), Expect = 5.732e-27
Identity = 96/338 (28.40%), Postives = 160/338 (47.34%), Query Frame = 3
Query:   60 LIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA 1073
            L+   G N+ NA D+KD      L +A    +LD++ + +    +++ K   G T  H A+      + K + S  + I+  +   N+ LH A  N +  +V  L+ +GAN++  N    TP+  A    +A  + +  ++ L+   AD+NI+  D K+  H   V  +    + + +N  E      INS D    +PLH A   G+  +   L+ +GA+      +   PLH+AA   H D  + LL+S  + +   DS   + +H AA  GN +  + L+  G +    D+ GR  L +AA S     +E L+   T++N  DQ   +ALH AAA
Sbjct:  158 LLLAKGANI-NAFDKKDGRA---LHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFA----AASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGE------INSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQID-TPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAA 480          

HSP 2 Score: 115.931 bits (289), Expect = 5.064e-26
Identity = 95/333 (28.53%), Postives = 159/333 (47.75%), Query Frame = 3
Query:  192 DINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARS--GCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCL-WTSGAILSDNMFGQSPLDIAISNNQE 1181
            DIN   A+ +T  H A+ +    + +L+      +N  DN   +PLH A+ +   D V+VL++  A++N  +K++QTP+ +A    +AL+    II LL      +N+ D   +T  H+  +    EM+  +    +       IN+ D  D   LH+A  +G+L++  VL+  GA  +        PLH AA  G + +VK LL+ S + +   ++  ++A+H+A  NG   +   LI  G +    +  G   L FAA S  G L  +E+L+    ++N+  +   + LH  A  G     Q L    G I S +  G +PL +A     E
Sbjct:   33 DINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAAAN-NALRCAEVIIPLLT----SVNVSDRGGRTALHHAALNGHTEMVNLLLAKGAN------INAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEID-EPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGAL-CLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHE 352          

HSP 3 Score: 98.9821 bits (245), Expect = 1.036e-20
Identity = 97/400 (24.25%), Postives = 175/400 (43.75%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCN-KDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYE---NISESQ-------RIELINSQDIVDK-----------------SPLHYAVSIGNLEIC-QVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLL-NSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFL 1202
            ++ KDN     L  AV +++ D +++ +    D+N +  + +T  H A+  N     +++     ++NV D    + LHHA +N   +MV +LL  GANIN  + KD +       +  +A      ++ +L+   A+++ +D    T  H      +I ++K +      I E          +   N QD V                   +PLH+A +  +  +C + L+  GA+ NI + +   PLH+ A  G     + L+ N    +   +D N  + +H+AA  G+  +   LI  G DC  +   G   L  AA +        LL+    ++  D +  T LH AAA G++  VQ L  SG   +  +  G++PL  A ++   + ++  +
Sbjct:   67 VNAKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRA------LHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGN--TPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLV 458          

HSP 4 Score: 93.5893 bits (231), Expect = 4.562e-19
Identity = 100/444 (22.52%), Postives = 172/444 (38.74%), Query Frame = 3
Query:   45 F*SSKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIE-----------LINS------QDIVDKSPLHYAVSIGNLEICQVLLEA--GANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEF-------------------------------------GRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            F  S+ + +NG  + +     D DG   L  A +  +  +I   +    D   +   G    H A++  +    + + S    I+  D+   + LH A     +D V++LLK G   N  +K  +TP     +  +A       ++ L++   D+N  D   ++  HY    +     +   E  SE  + E           L+ S      +D    SP+HYA + G+    ++LL+   G        N   PLH+AA  GH   +++LL   R+ +  RD    + + +AA  G+ +    L+  G   L+ D+                                      GR  LM A   G +  +  LL +E NVN  D+  + ALH A      + VQCL    A +L  +  G++P+ +A +    + +   L 
Sbjct:  319 FTRSQTLIQNGGEINSV----DKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTP-----LHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVD-ERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLT 752          

HSP 5 Score: 90.5077 bits (223), Expect = 4.442e-18
Identity = 74/228 (32.46%), Postives = 112/228 (49.12%), Query Frame = 3
Query:  549 LVVKEKIEMIKWIYENISESQRIELINSQDI-----VDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCL---PLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            L + ++  +I+ I+    E  R+ +  S+DI       ++PLH A  +G+ EI ++L+ +GA  N   A D +   PLH A      D V++L+  S      RD N  + +H+AA N      + +I +       D  GR  L  AA +G   ++  LL K  N+N  D+    ALH AA  GHL VV  L + GA +S  +  G +PL  A S+ Q  VVK  L+
Sbjct:    4 LKLADQPALIQAIFSGDPEEIRMLIYKSEDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVN---AKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNA-RDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLS 227          

HSP 6 Score: 80.1073 bits (196), Expect = 6.283e-15
Identity = 101/440 (22.95%), Postives = 169/440 (38.41%), Query Frame = 3
Query:  132 SYAVQNKNLDIIKICLDFCVDINTKQA----DGKTIFHTASIINY----PILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKAD-LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPN--------------IFAANDCL-------------------PLHIAAEKGHLDIVKLLLN-------------------------------------SSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-GIGKDCLA-KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            S  VQ +       CL+F +      A     G +  H A+   +     +L      R    + G+    SPLH A  +     ++VLL+ G  ++  ++  +TP+ LA     AL+  +  +  L+ + A  L+      +T  H  V+      ++ +   + E    E+++  D   ++PL  AV  G+++   +LLE  AN N              + A ++C+                   P+H+AA +GH   +  LL                                        R   C  D N  + +H A  NG+      L+  +G D    +D  GR  L  AA +G    I+ LL+ +  V+  D    T L  AAA+G +  ++ L  +GA LS  +  G +PL +A SN  E+ V   L 
Sbjct:  495 SEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGN--AQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALA-----ALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLL---LDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRCI-DGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVACSNGTEDGVLMILQ 923          

HSP 7 Score: 62.003 bits (149), Expect = 2.425e-9
Identity = 81/351 (23.08%), Postives = 142/351 (40.46%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANIN-----LCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRK-SECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCL---AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVT-ALHQAAAEGHLQVVQCLWTSGA 1118
            LD  D  G+  L  AV   ++D + + L+   ++NT    G    H A +       + +  +D  +  GD+   +P+H A        +  LL    + +     L +    TP+  AC          G +++L +E+      D +  T  H  VV         + + +      ++   +D   ++PLH A   G  E  Q+LL   A  +    +   PL  AA KG +  ++ LL++    S   RD N  + +H+A  NG  +    ++    D     A +   +  L  AA+ G    ++ LL++  +    D+  +T AL  A +    QV  CL  SG+
Sbjct:  658 LDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWAC-----FYGQDGCVEVL-LEQRGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGS----DITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGN--TPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQTVDEDGLTPALACAPSR---QVADCLAHSGS 993          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Match: ankrd44 (ankyrin repeat domain 44 [Source:NCBI gene;Acc:101173198])

HSP 1 Score: 118.627 bits (296), Expect = 7.364e-27
Identity = 96/338 (28.40%), Postives = 160/338 (47.34%), Query Frame = 3
Query:   60 LIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAA 1073
            L+   G N+ NA D+KD      L +A    +LD++ + +    +++ K   G T  H A+      + K + S  + I+  +   N+ LH A  N +  +V  L+ +GAN++  N    TP+  A    +A  + +  ++ L+   AD+NI+  D K+  H   V  +    + + +N  E      INS D    +PLH A   G+  +   L+ +GA+      +   PLH+AA   H D  + LL+S  + +   DS   + +H AA  GN +  + L+  G +    D+ GR  L +AA S     +E L+   T++N  DQ   +ALH AAA
Sbjct:  108 LLLAKGANI-NAFDKKDGRA---LHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFA----AASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGE------INSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQID-TPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAA 430          

HSP 2 Score: 102.064 bits (253), Expect = 1.046e-21
Identity = 102/433 (23.56%), Postives = 180/433 (41.57%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACM--EISALKNL--------------------------SGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEM-IKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESS-AIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLE 1301
            ++ +D + Q  L  A  N  L   ++ +     +N     G+T  H A++  +  +  L+ ++  NIN  D      LH A     LD+V VL+  GA ++  +K   TP+  A    +I+ +K+L                            ++  LI   A+++  +    T  H+        + ++++  N ++      IN Q    KSPLH     G     Q L++ G   N    +   PLH+AA  GH  ++  L+ S   ++C R        +H+AA N + +  + L+  G      D  GR  L  AA  G +  ++ LL      N  D+   T LH AAA  H   ++ L   G  I + + +G+S L  A +++ +   +  L  D+ + V A +    +  +E L++S     L+
Sbjct:   50 VNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGAD------INIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSG--ADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDS-EGVQAEKEKEAAQCLEFLLQSGAAAALK 473          

HSP 3 Score: 92.8189 bits (229), Expect = 7.704e-19
Identity = 100/444 (22.52%), Postives = 172/444 (38.74%), Query Frame = 3
Query:   45 F*SSKLIKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIE-----------LINS------QDIVDKSPLHYAVSIGNLEICQVLLEA--GANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEF-------------------------------------GRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA-ILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            F  S+ + +NG  + +     D DG   L  A +  +  +I   +    D   +   G    H A++  +    + + S    I+  D+   + LH A     +D V++LLK G   N  +K  +TP     +  +A       ++ L++   D+N  D   ++  HY    +     +   E  SE  + E           L+ S      +D    SP+HYA + G+    ++LL+   G        N   PLH+AA  GH   +++LL   R+ +  RD    + + +AA  G+ +    L+  G   L+ D+                                      GR  LM A   G +  +  LL +E NVN  D+  + ALH A      + VQCL    A +L  +  G++P+ +A +    + +   L 
Sbjct:  269 FTRSQTLIQNGGEINSV----DKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTP-----LHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVD-ERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLT 702          

HSP 4 Score: 80.1073 bits (196), Expect = 7.651e-15
Identity = 101/440 (22.95%), Postives = 169/440 (38.41%), Query Frame = 3
Query:  132 SYAVQNKNLDIIKICLDFCVDINTKQA----DGKTIFHTASIINY----PILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKAD-LNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPN--------------IFAANDCL-------------------PLHIAAEKGHLDIVKLLLN-------------------------------------SSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-GIGKDCLA-KDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            S  VQ +       CL+F +      A     G +  H A+   +     +L      R    + G+    SPLH A  +     ++VLL+ G  ++  ++  +TP+ LA     AL+  +  +  L+ + A  L+      +T  H  V+      ++ +   + E    E+++  D   ++PL  AV  G+++   +LLE  AN N              + A ++C+                   P+H+AA +GH   +  LL                                        R   C  D N  + +H A  NG+      L+  +G D    +D  GR  L  AA +G    I+ LL+ +  V+  D    T L  AAA+G +  ++ L  +GA LS  +  G +PL +A SN  E+ V   L 
Sbjct:  445 SEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGN--AQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALA-----ALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLL---LDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRCI-DGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVACSNGTEDGVLMILQ 873          

HSP 5 Score: 66.6254 bits (161), Expect = 1.011e-10
Identity = 50/142 (35.21%), Postives = 70/142 (49.30%), Query Frame = 3
Query:  783 LDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS-DNMFGQSPLDIAISNNQENVVKFFLN 1205
            LD V++L+  S      RD N  + +H+AA N      + +I +       D  GR  L  AA +G   ++  LL K  N+N  D+    ALH AA  GHL VV  L + GA +S  +  G +PL  A S+ Q  VVK  L+
Sbjct:   37 LDAVQVLIRHSADVN-ARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLS 177          

HSP 6 Score: 62.003 bits (149), Expect = 2.852e-9
Identity = 81/351 (23.08%), Postives = 142/351 (40.46%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANIN-----LCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRK-SECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCL---AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVT-ALHQAAAEGHLQVVQCLWTSGA 1118
            LD  D  G+  L  AV   ++D + + L+   ++NT    G    H A +       + +  +D  +  GD+   +P+H A        +  LL    + +     L +    TP+  AC          G +++L +E+      D +  T  H  VV         + + +      ++   +D   ++PLH A   G  E  Q+LL   A  +    +   PL  AA KG +  ++ LL++    S   RD N  + +H+A  NG  +    ++    D     A +   +  L  AA+ G    ++ LL++  +    D+  +T AL  A +    QV  CL  SG+
Sbjct:  608 LDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWAC-----FYGQDGCVEVL-LEQRGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGS----DITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGN--TPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQTVDEDGLTPALACAPSR---QVADCLAHSGS 943          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078036.1 (SMESG000078036.1)

HSP 1 Score: 974.156 bits (2517), Expect = 0.000e+0
Identity = 480/497 (96.58%), Postives = 483/497 (97.18%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIK----WI--YENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTI 1538
            KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIK    WI   +NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKW  +   F+ I
Sbjct:  279 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKVIIYWITYLKNISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWFVYLFKFYFI 775          

HSP 2 Score: 243.047 bits (619), Expect = 1.881e-68
Identity = 118/178 (66.29%), Postives = 142/178 (79.78%), Query Frame = 3
Query: 1827 LALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQR 2360
            +AL+ I +PSKLPCL E +K PG+IFLSYF F+YYLQHLP VGIQ+IMF KVLKTMI+                       DPFKN+ L+YMKSLAMMTGEFDLVTYF++GKKNYYVTS++MISLFV+IMSI+LFNLIIGLAVGDIG++ASEATM+AYKLKVGY +EVE+ VP  +++
Sbjct:    1 MALLFIDDPSKLPCLQESQKAPGIIFLSYFQFLYYLQHLPVVGIQIIMFLKVLKTMIK-----------------------DPFKNIALSYMKSLAMMTGEFDLVTYFLTGKKNYYVTSIVMISLFVIIMSITLFNLIIGLAVGDIGEMASEATMEAYKLKVGYFLEVESRVPKALKK 155          

HSP 3 Score: 77.411 bits (189), Expect = 2.928e-14
Identity = 103/413 (24.94%), Postives = 174/413 (42.13%), Query Frame = 3
Query:  138 AVQNKNLDIIKI-CLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISAL-------------------------------------KNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLP-----LHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-------GIGKDCL-----AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTS---GAILSDNMFGQSPLDIAISNNQENVVKFFL 1202
            A Q  +++II++ C    + I+     G+T  H +    +  + K++     +++  ++D  S +HH +  N L+M+ +L  + A +N+ N + QTPI++A +E  A                                      KNL  IIK+ +    D+N +  D KTIFH   +     + K +     +S+ I  IN  D    SPLH+A+    L++ +VLL+ GAN N+   +   P     + I+A K    I+KLL+   +     RD +  +  H        E+ K +I        I +        ++D   ++ L +A   G L I + LL    N N+        LH AA +GHL +V+ L  S        D+    S + IA  N    + KF +
Sbjct:  162 ASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLD-IIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDIN-INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLI-IEKADLNIRDYDMKTIFHYLVVKEKIEMIKVIIYWITYLKNISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSN-ESSAIHIAAENGNYEIFKFLI 565          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078036.1 (SMESG000078036.1)

HSP 1 Score: 968.763 bits (2503), Expect = 0.000e+0
Identity = 480/497 (96.58%), Postives = 483/497 (97.18%), Query Frame = 3
Query:   66 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIK----WI--YENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTI 1538
            KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDF VDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIK    WI   +NISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKW  +   F+ I
Sbjct:  199 KENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKVIIYWITYLKNISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWFVYLFKFYFI 695          

HSP 2 Score: 76.6406 bits (187), Expect = 4.750e-14
Identity = 102/412 (24.76%), Postives = 174/412 (42.23%), Query Frame = 3
Query:  138 AVQNKNLDIIKI-CLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISAL-------------------------------------KNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLP-----LHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-------GIGKDCL-----AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSG--AILSDNMFGQSPLDIAISNNQENVVKFFL 1202
            A Q  +++II++ C    + I+     G+T  H +    +  + K++     +++  ++D  S +HH +  N L+M+ +L  + A +N+ N + QTPI++A +E  A                                      KNL  IIK+ +    D+N +  D KTIFH   +     + K +     +S+ I  IN  D    SPLH+A+    L++ +VLL+ GAN N+   +   P     + I+A K    I+KLL+   +     RD +  +  H        E+ K +I        I +        ++D   ++ L +A   G L I + LL    N N+        LH AA +GHL +V+ L  S   +    +    S + IA  N    + KF +
Sbjct:   82 ASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLESDEISVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLD-IIKICLDFYVDINTKQADGKTIFHTASIINYPILFKLM-----KSRDIN-INVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLI-IEKADLNIRDYDMKTIFHYLVVKEKIEMIKVIIYWITYLKNISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI 485          

HSP 3 Score: 66.6254 bits (161), Expect = 6.556e-11
Identity = 55/196 (28.06%), Postives = 87/196 (44.39%), Query Frame = 3
Query:  651 SPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDC--LAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLW---------TSGAILSDNMFGQSPLDIAISNNQENVVKFFLN 1205
            S LHYAV   +L+I   LLE   N NI A     P+H+A++KGH++I++LL           D    +A+HI+   G+ E+ K L+  G     L  DE     +    R   L +I  L      +N+ +    T +  AA EG+ ++   L             A+   +  GQ  L  A+ N   +++K  L+
Sbjct:   44 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQKGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLESDEIS--VMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLD 237          

HSP 4 Score: 64.6994 bits (156), Expect = 2.234e-10
Identity = 72/275 (26.18%), Postives = 129/275 (46.91%), Query Frame = 3
Query:  321 SPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKA-DLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSEC-------FRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLL-TKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGA 1118
            S LH+A++ N LD++  LL+F  NIN+       PI LA       K    II+LL  +K+  ++  D   +T  H    +   E++K + +N +   ++E   S +I   S +H+ V   +LE+  +L    A  N+   N   P+ IAA +G+ ++  +L+   +++          +D++    +  A +N N +I K  +    D   K   G+ T+   A     PI+  L+ +++ N+N+ D    + LH A     L +V+ L   GA
Sbjct:   44 SLLHYAVLENHLDILSFLLEFKPNINIIALKKVRPIHLASQ-----KGHVEIIELLCKQKSLYIDATDDKGQTALHISCRRGHKEVLKVLLQNGASVHKLE---SDEI---SVMHHPVRRNDLEMINILFLYNAPLNVKNNNGQTPIMIAAIEGYAELFSILILKFKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFYVDINTKQADGK-TIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGA 306          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078033.1 (SMESG000078033.1)

HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0
Identity = 425/801 (53.06%), Postives = 584/801 (72.91%), Query Frame = 3
Query:   63 IKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLF-QNSFIFTKSE 2462
            +K+ G N+ + L+EKDNDG  CLS+AVQN+N +IIKIC+   VD++ KQ DGKTIFH+A+II Y  LF L+++   ++N+ DN+LN+P+HHAI+NN L MVK+LL  GANIN+CN+ FQTPI+LAC+EI+  +N + IIK L    A+L I+D++MKTIFHYL+ KE IEM+++  + ++    +ELINSQD    SPLH A  IGN EICQ+LL+AGA+P +F+ ND L LH+A  KGHLD V +LL +S+++  +RD+NE+SAI++AAENG YEIFK L+ IGKD ++KD+FGRNTLMFAA+SGCLPI+E LL  E N+NL+DQMK TALHQAA  GHL +V+ LW  GA LS++MFG + LD+AI N Q +VV+FFLN++NW+ VM +RH NG  HI+ LIK NE++TLELLDKCIIEN SN MDPNYE+++ F ++D+GPDD ASIS+RY ALKSM +  +L LL+HPLS KLL+ KW++FG+ F+ IDF+++L+++I I  ++     + D+DS    +GCK  NSS  +S+K  A    V  N LI  I+  + ++L++ L  Q+ R   E W   +   + L    I NP +LPCLYEW+  PGLIF+ +FL +YY++  P +GIQ++MFQKVL ++I+    FF +F GF FGFYIIN  QDPFK  GL  +KS+AMM GE ++V+YFV+GKKNY   S  MI  F++IM I+ FNL+IGLAVGDI ++ +EA ++  KL+V ++ E+E+ +P   QR LY SEI +     KK  I++ +F +NS I    E
Sbjct:  237 MKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYHLNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIKTLNQANANLKIKDFEMKTIFHYLIEKENIEMLEYFTQTLNSEILLELINSQDDSHNSPLHCAAKIGNAEICQILLKAGADPFLFSQNDFLALHVATIKGHLDCVIILLEASKETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRNTLMFAAKSGCLPIVEDLLNDEVNINLYDQMKSTALHQAAERGHLDIVKLLWKQGAKLSEDMFGSTQLDVAILNQQWHVVRFFLNNENWKKVMNFRHQNGHGHIKVLIKYNEEITLELLDKCIIENMSNVMDPNYEIKFWFGILDSGPDDKASISKRYLALKSMADCGQLDLLKHPLSEKLLQCKWNKFGLAFYLIDFIVYLLFLICINVYMSIYTPMTDTDSYTSYSGCKQTNSSV-SSDKLDAPVGVVLMNSLIYAIITGQLLLLLLKLFSQRIRFFMEVWNYIFITNDILVFFFIKNPLQLPCLYEWQFAPGLIFVVWFLLLYYMRFFPYIGIQVMMFQKVLVSIIKAFAIFFGLFVGFGFGFYIINSSQDPFKTQGLGIIKSIAMMIGELEIVSYFVTGKKNYANVSYAMIVCFMIIMPITFFNLMIGLAVGDISNVNAEAELEMIKLRVNFVWEMESQLPVRWQRILYVSEITEHSHNFKKFKILNKIFSKNSNIIQDKE 1036          

HSP 2 Score: 83.5741 bits (205), Expect = 4.371e-16
Identity = 106/434 (24.42%), Postives = 190/434 (43.78%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLL----KFGANI--NLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAA-----EKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-GIGKDCL-----AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS--DNMFGQSPLDIAISNNQENVVKFFLN--------DDNWQNVMAYRHDNGSSHI-ENLIKSNEDLTLELLDK 1313
            +D  D  GQ  L  A +  +L+++KI L +   +   + D  ++ H     N   + K++ +    +NV +N+  +PL  A I    ++  +LL    K G NI   L  KD       +C+  +     S IIK+ I    DL+ +  D KTIFH   +    E+   I  N         +N  D    +P+H+A+   NL++ ++LL+ GAN N+   +   P+ +A       +   +I+K  LN +  +   +D    +  H   E  N E+ ++    +  + L     ++D+   + L  AA+ G   I + LL    +  L  Q    ALH A  +GHL  V  L  +    S   +    S +++A  N +  + K  ++        DD  +N + +   +G   I E+L+  N+++ + L D+
Sbjct:  141 IDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDG---FSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYH------LNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIK-TLNQANANLKIKDFEMKTIFHYLIEKENIEMLEYFTQTLNSEILLELINSQDDSHNSPLHCAAKIGNAEICQILLKAGADPFLFSQNDFLALHVATIKGHLDCVIILLEASKETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRNTLMFAAKSGCLPIVEDLL--NDEVNINLYDQ 562          

HSP 3 Score: 61.6178 bits (148), Expect = 2.615e-9
Identity = 60/252 (23.81%), Postives = 109/252 (43.25%), Query Frame = 3
Query:  480 KLLIIEKADLNIRDYDMKTIFH-----YLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF---------GQSPLDIAISNNQENVVK 1193
            K+L I+K D +I  Y  K +FH     YL   + + +  W       ++R+ +I+ ++  + S LHYA    + +I   LL    + N+  AN   P+H+AA++GH++IV+LL           D    + +H A   G+  + K L+         +    + +    R     +++ L+   + +N+ +    T L  AA EGH ++   L        +N+          G S L  A+ N    ++K
Sbjct:   32 KVLKIQKLD-DI-SYQTKQLFHAVQHGYLQFLQSVPLKVW-------AKRLNVIDEKN--NFSLLHYACLENHKDIVSFLLIFKPDVNVIGANKIRPIHLAAQQGHMEIVELLCKYKSTHIDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIK 272          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078033.1 (SMESG000078033.1)

HSP 1 Score: 639.032 bits (1647), Expect = 0.000e+0
Identity = 312/556 (56.12%), Postives = 423/556 (76.08%), Query Frame = 3
Query:   63 IKENGINVKNALDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISNNQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCIIENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLRHPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIV 1730
            +K+ G N+ + L+EKDNDG  CLS+AVQN+N +IIKIC+   VD++ KQ DGKTIFH+A+II Y  LF L+++   ++N+ DN+LN+P+HHAI+NN L MVK+LL  GANIN+CN+ FQTPI+LAC+EI+  +N + IIK L    A+L I+D++MKTIFHYL+ KE IEM+++  + ++    +ELINSQD    SPLH A  IGN EICQ+LL+AGA+P +F+ ND L LH+A  KGHLD V +LL +S+++  +RD+NE+SAI++AAENG YEIFK L+ IGKD ++KD+FGRNTLMFAA+SGCLPI+E LL  E N+NL+DQMK TALHQAA  GHL +V+ LW  GA LS++MFG + LD+AI N Q +VV+FFLN++NW+ VM +RH NG  HI+ LIK NE++TLELLDKCIIEN SN MDPNYE+++ F ++D+GPDD ASIS+RY ALKSM +  +L LL+HPLS KLL+ KW++FG+ F+ IDF+++L+++I I  ++     + D+DS    +GCK  NSS  +S+K  A    V  N LI  I+
Sbjct:  237 MKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYHLNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIKTLNQANANLKIKDFEMKTIFHYLIEKENIEMLEYFTQTLNSEILLELINSQDDSHNSPLHCAAKIGNAEICQILLKAGADPFLFSQNDFLALHVATIKGHLDCVIILLEASKETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRNTLMFAAKSGCLPIVEDLLNDEVNINLYDQMKSTALHQAAERGHLDIVKLLWKQGAKLSEDMFGSTQLDVAILNQQWHVVRFFLNNENWKKVMNFRHQNGHGHIKVLIKYNEEITLELLDKCIIENMSNVMDPNYEIKFWFGILDSGPDDKASISKRYLALKSMADCGQLDLLKHPLSEKLLQCKWNKFGLAFYLIDFIVYLLFLICINVYMSIYTPMTDTDSYTSYSGCKQTNSSV-SSDKLDAPVGVVLMNSLIYAII 791          

HSP 2 Score: 80.8777 bits (198), Expect = 2.522e-15
Identity = 106/434 (24.42%), Postives = 190/434 (43.78%), Query Frame = 3
Query:   96 LDEKDNDGQCCLSYAVQNKNLDIIKICLDFCVDINTKQADGKTIFHTASIINYPILFKLMKSRDININVGDNDLNSPLHHAIINNKLDMVKVLL----KFGANI--NLCNKDFQTPILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAA-----EKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLI-GIGKDCL-----AKDEFGRNTLMFAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILS--DNMFGQSPLDIAISNNQENVVKFFLN--------DDNWQNVMAYRHDNGSSHI-ENLIKSNEDLTLELLDK 1313
            +D  D  GQ  L  A +  +L+++KI L +   +   + D  ++ H     N   + K++ +    +NV +N+  +PL  A I    ++  +LL    K G NI   L  KD       +C+  +     S IIK+ I    DL+ +  D KTIFH   +    E+   I  N         +N  D    +P+H+A+   NL++ ++LL+ GAN N+   +   P+ +A       +   +I+K  LN +  +   +D    +  H   E  N E+ ++    +  + L     ++D+   + L  AA+ G   I + LL    +  L  Q    ALH A  +GHL  V  L  +    S   +    S +++A  N +  + K  ++        DD  +N + +   +G   I E+L+  N+++ + L D+
Sbjct:  141 IDAADVKGQTPLHTACRRGHLNVVKILLKYQASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDG---FSCLSHAVQNENSEIIKICIGNAVDLDAKQNDGKTIFHSAAIIGYSELFDLIETNSYH------LNITDNELNTPIHHAIMNNNLKMVKLLLDKGANINVCNRHFQTPIMLACIEITQSRNTAEIIK-TLNQANANLKIKDFEMKTIFHYLIEKENIEMLEYFTQTLNSEILLELINSQDDSHNSPLHCAAKIGNAEICQILLKAGADPFLFSQNDFLALHVATIKGHLDCVIILLEASKETSYYRDANENSAINLAAENGRYEIFKHLVDIGKDKMSKDDFGRNTLMFAAKSGCLPIVEDLL--NDEVNINLYDQ 562          

HSP 3 Score: 59.6918 bits (143), Expect = 8.626e-9
Identity = 62/253 (24.51%), Postives = 112/253 (44.27%), Query Frame = 3
Query:  480 KLLIIEKADLNIRDYDMKTIFH-----YLVVKEKIEMIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGANPNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAAENGNYEIFKFLIGIGKDCLAKDEFGRNTLM-FAARSGCLPIIEYLLTKETNVNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMF---------GQSPLDIAISNNQENVVK 1193
            K+L I+K  L+   Y  K +FH     YL   + + +  W       ++R+ +I+ ++  + S LHYA    + +I   LL    + N+  AN   P+H+AA++GH++IV+LL           D    + +H A   G+  + K L+   +  + K E    ++M    R     +++ L+   + +N+ +    T L  AA EGH ++   L        +N+          G S L  A+ N    ++K
Sbjct:   32 KVLKIQK--LDDISYQTKQLFHAVQHGYLQFLQSVPLKVW-------AKRLNVIDEKN--NFSLLHYACLENHKDIVSFLLIFKPDVNVIGANKIRPIHLAAQQGHMEIVELLCKYKSTHIDAADVKGQTPLHTACRRGHLNVVKILLKY-QASVQKLELDSISVMHHPVRRNDTDMVKVLVAYGSPLNVRNNNGQTPLMVAAIEGHFELFDLLLNEMKKQGENIISILNEKDNDGFSCLSHAVQNENSEIIK 272          
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Match: SMESG000078034.1 (SMESG000078034.1)

HSP 1 Score: 592.423 bits (1526), Expect = 0.000e+0
Identity = 307/312 (98.40%), Postives = 312/312 (100.00%), Query Frame = 3
Query: 1521 MVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLXXXXXXXXXXXXXXYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSFIFTK 2456
            MVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLFSFFTIFFGFAFGFYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNS+++++
Sbjct:    1 MVFFTIDFLLFLVYIILITYFVLSNERILDSDSVDVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQKFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLFIYYLQHLPSVGIQLIMFQKVLKTMIRVLFSFFTIFFGFAFGFYIINQDQDPFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSISLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYHSEICDRPKQHKKRFIVSWLFQNSYLYSR 312          
The following BLAST results are available for this feature:
BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRPA14.242e-5324.39transient receptor potential cation channel subfam... [more]
TRPA12.510e-5224.51transient receptor potential cation channel subfam... [more]
RIPK48.907e-3026.49receptor interacting serine/threonine kinase 4 [So... [more]
RIPK49.119e-3026.69receptor interacting serine/threonine kinase 4 [So... [more]
ANKRD508.616e-2926.39ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trpa-15.366e-5824.86Transient receptor potential cation channel subfam... [more]
trpa-15.887e-5824.86Transient receptor potential cation channel subfam... [more]
unc-441.130e-2728.25AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.130e-2728.25AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.716e-2728.14AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TrpA12.861e-7026.22gene:FBgn0035934 transcript:FBtr0331825[more]
TrpA14.549e-7026.22gene:FBgn0035934 transcript:FBtr0331823[more]
TrpA15.887e-7026.17gene:FBgn0035934 transcript:FBtr0331824[more]
TrpA18.544e-7025.93gene:FBgn0035934 transcript:FBtr0331826[more]
TrpA11.663e-6525.89gene:FBgn0035934 transcript:FBtr0331827[more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trpa1a2.476e-5524.07transient receptor potential cation channel, subfa... [more]
trpa1b3.233e-5423.87transient receptor potential cation channel, subfa... [more]
trpa1b3.233e-5423.87transient receptor potential cation channel, subfa... [more]
trpa1b1.555e-5123.61transient receptor potential cation channel, subfa... [more]
ripk41.186e-3027.51receptor-interacting serine-threonine kinase 4 [So... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trpa19.180e-6226.94transient receptor potential cation channel, subfa... [more]
trpa12.179e-6125.77transient receptor potential cation channel, subfa... [more]
trpa14.915e-6126.62transient receptor potential cation channel, subfa... [more]
trpa18.719e-6127.01transient receptor potential cation channel, subfa... [more]
trpa13.382e-5425.63transient receptor potential cation channel, subfa... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Trpa11.216e-5124.56transient receptor potential cation channel, subfa... [more]
Trpa13.264e-4924.05transient receptor potential cation channel, subfa... [more]
Ank11.164e-2829.80ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
Ankrd502.228e-2826.61ankyrin repeat domain 50 [Source:MGI Symbol;Acc:MG... [more]
Ankrd288.058e-2827.90ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7Z020|TRPA1_DROME2.868e-6926.22Transient receptor potential cation channel subfam... [more]
sp|Q18297|TRPA1_CAEEL7.488e-5724.86Transient receptor potential cation channel subfam... [more]
sp|O75762|TRPA1_HUMAN1.205e-5124.51Transient receptor potential cation channel subfam... [more]
sp|Q8BLA8|TRPA1_MOUSE8.503e-5124.56Transient receptor potential cation channel subfam... [more]
sp|Q6RI86|TRPA1_RAT6.994e-5024.55Transient receptor potential cation channel subfam... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E8991.067e-11031.16ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267FPG61.930e-11031.16ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267G9R92.215e-10330.46ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267EGL52.948e-10330.46ANK_REP_REGION domain-containing protein OS=Macros... [more]
A0A267H4S25.492e-10330.33ANK_REP_REGION domain-containing protein OS=Macros... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trpa1b8.129e-5624.81transient receptor potential cation channel subfam... [more]
trpa1b9.193e-5624.81transient receptor potential cation channel subfam... [more]
trpa1b9.193e-5624.81transient receptor potential cation channel subfam... [more]
trpa1a1.970e-5323.84transient receptor potential cation channel subfam... [more]
trpa1a1.422e-5024.02transient receptor potential cation channel subfam... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000095.12.122e-3026.65pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000000093.11.867e-2926.77pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000007446.13.023e-2624.25pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000003991.12.487e-2527.30pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1... [more]
ENSPMAT00000002048.11.022e-2426.24pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 g... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
NAS66.657e-1632.37Evolutionarily conserved 19S regulatory particle a... [more]
AVO23.178e-932.35Component of a complex containing the Tor2p kinase... [more]
HOS41.289e-831.75Subunit of the Set3 complex; complex is a meiotic-... [more]
AKR28.485e-828.65Ankyrin repeat-containing protein; member of a fam... [more]
PHO813.457e-624.78Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO391127.364e-3030.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO273542.122e-2723.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO331805.315e-2225.42Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387021.424e-1825.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494853.575e-1627.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trpa1b9.535e-5524.68transient receptor potential cation channel subfam... [more]
ank1a6.525e-3327.67ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ank1b1.445e-3026.98ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ankrd445.732e-2728.40ankyrin repeat domain 44 [Source:NCBI gene;Acc:101... [more]
ankrd447.364e-2728.40ankyrin repeat domain 44 [Source:NCBI gene;Acc:101... [more]
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BLAST of Transient receptor potential cation channel subfamily A member 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078036.11.881e-6896.58SMESG000078036.1[more]
SMESG000078036.14.750e-1496.58SMESG000078036.1[more]
SMESG000078033.14.371e-1653.06SMESG000078033.1[more]
SMESG000078033.12.522e-1556.12SMESG000078033.1[more]
SMESG000078034.10.000e+098.40SMESG000078034.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005445 ID=SMED30005445|Name=Transient receptor potential cation channel subfamily A member 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2619bp
ATTGCATTTAGTCTATTTTAAATTTAAATAATTGGTTGTTAAATTTCTAA
TCTTCGAAACTTATCAAAGAAAATGGCATCAATGTCAAAAATGCACTTGA
TGAAAAAGACAATGATGGGCAGTGTTGCCTTTCTTATGCAGTTCAAAATA
AAAATCTTGATATAATTAAAATATGTCTGGATTTCTGTGTAGATATAAAT
ACAAAACAAGCTGATGGTAAAACAATATTTCACACTGCTTCAATAATTAA
TTATCCAATTCTTTTTAAATTGATGAAATCAAGGGATATTAATATCAATG
TCGGCGATAATGATTTGAATTCACCTCTTCATCATGCCATTATTAACAAT
AAGTTGGATATGGTTAAAGTTTTGTTGAAATTCGGTGCCAATATTAATTT
GTGCAACAAAGATTTCCAAACTCCAATCTTACTTGCGTGCATGGAAATAA
GCGCTTTAAAAAATCTTTCGGGTATTATTAAACTGTTAATCATTGAAAAA
GCCGACTTGAATATCCGAGATTATGATATGAAAACAATTTTTCATTATTT
GGTGGTAAAAGAGAAGATTGAAATGATCAAGTGGATATATGAGAATATTT
CTGAAAGCCAACGAATAGAGCTTATTAATTCTCAAGACATCGTTGATAAA
AGTCCCCTTCATTATGCAGTATCTATTGGAAATTTAGAAATTTGTCAAGT
ACTTTTGGAAGCGGGCGCAAATCCAAATATTTTTGCCGCAAACGATTGCT
TGCCTTTGCATATTGCTGCAGAAAAGGGCCATTTGGATATTGTGAAGTTG
TTATTAAATTCAAGCAGGAAATCGGAATGCTTCCGAGATAGCAATGAAAG
TTCTGCAATTCACATTGCAGCTGAAAATGGAAATTACGAAATTTTCAAAT
TTCTGATCGGGATAGGCAAAGATTGCCTTGCAAAAGATGAATTCGGTAGA
AATACATTAATGTTCGCGGCTCGGAGTGGATGTTTACCAATTATTGAATA
TTTGTTAACAAAAGAAACTAATGTTAATTTACATGATCAAATGAAAGTAA
CTGCTCTGCATCAAGCTGCTGCTGAAGGACATTTACAGGTCGTTCAATGT
TTGTGGACAAGTGGAGCCATTCTTTCCGATAACATGTTTGGACAATCTCC
GTTAGATATTGCAATTTCGAACAATCAAGAAAATGTGGTGAAATTTTTTC
TGAATGATGACAATTGGCAAAATGTGATGGCTTATCGCCATGATAATGGC
AGCAGCCACATTGAAAATTTGATAAAATCGAATGAAGATTTAACATTAGA
GCTTTTAGACAAATGTATCATAGAAAATACTAGCAATAAAATGGATCCGA
ATTATGAAGTACAATATCGGTTCAGTTTAATTGATGCCGGCCCTGACGAC
CCTGCAAGTATAAGTGAACGATATCACGCGTTAAAGAGCATGACAGAATA
CAAACGATTAAAATTATTGAGACATCCTTTGTCTGCAAAATTGTTAAAAA
TTAAATGGGATCAATTTGGAATGGTATTTTTCACAATAGATTTCCTTTTG
TTTCTTGTTTACATTATATTGATAACGTATTTTGTTTTATCCAATGAACG
TATATTGGATTCAGATTCAGTTGATGTAGAAACTGGTTGCAAGATCAACA
ACTCTTCAACTCAAAATTCCGAGAAAAAAAGTGCATCTGCACCAAATGTC
ACGTCAAACATCCTGATCATAATGATTGTCGGAATAAGAAATATAATTTT
AGTGATGACATTAATTGTTCAGAAATTTCGAAATTTATTTGAATTTTGGA
CCAACTGTTATTTTGCATGCAACTCCTTAGCCTTAATTATTATTAGCAAC
CCATCCAAATTACCCTGTCTTTATGAGTGGGAGAAAGTACCCGGATTAAT
ATTTTTGTCGTATTTCCTATTTATATATTATCTGCAACATTTACCATCAG
TTGGCATTCAATTAATTATGTTCCAGAAAGTTCTAAAGACAATGATTAGA
GTTCTCTTTTCTTTTTTTACAATTTTTTTTGGATTCGCTTTTGGCTTTTA
TATAATTAATCAGGATCAGGATCCGTTTAAAAATCTTGGTTTAGCATATA
TGAAAAGTTTGGCAATGATGACAGGCGAATTTGATCTTGTTACGTATTTT
GTGTCTGGAAAGAAAAATTATTACGTTACGTCATTGATTATGATTTCTCT
TTTTGTTGTAATAATGTCAATCTCTCTTTTCAATTTAATTATTGGACTTG
CTGTTGGGGATATTGGTGATATCGCATCTGAGGCAACAATGCAGGCATAC
AAGCTTAAGGTCGGATATATAATGGAAGTAGAGACCAACGTTCCGACAGT
GATTCAACGATATCTATACCACTCAGAAATATGCGATCGTCCAAAACAAC
ATAAAAAACGTTTTATAGTTAGCTGGCTATTTCAAAACTCATTTATATTC
ACGAAAAGTGAATTTATGTTTAATGCATTAATCTGGTCACGATGAATTGA
TATAAATTTAGACTGTGATTTATCCATTTCTTTTATTTATTCTGTTTCTA
CACAATTGTATTTTGGGTACCCTCATTGATATACACTGTTGGAAAGAAAT
TTCGGCAAATGCCAATTTT
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protein sequence of SMED30005445-orf-1

>SMED30005445-orf-1 ID=SMED30005445-orf-1|Name=SMED30005445-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=741bp
MKSRDININVGDNDLNSPLHHAIINNKLDMVKVLLKFGANINLCNKDFQT
PILLACMEISALKNLSGIIKLLIIEKADLNIRDYDMKTIFHYLVVKEKIE
MIKWIYENISESQRIELINSQDIVDKSPLHYAVSIGNLEICQVLLEAGAN
PNIFAANDCLPLHIAAEKGHLDIVKLLLNSSRKSECFRDSNESSAIHIAA
ENGNYEIFKFLIGIGKDCLAKDEFGRNTLMFAARSGCLPIIEYLLTKETN
VNLHDQMKVTALHQAAAEGHLQVVQCLWTSGAILSDNMFGQSPLDIAISN
NQENVVKFFLNDDNWQNVMAYRHDNGSSHIENLIKSNEDLTLELLDKCII
ENTSNKMDPNYEVQYRFSLIDAGPDDPASISERYHALKSMTEYKRLKLLR
HPLSAKLLKIKWDQFGMVFFTIDFLLFLVYIILITYFVLSNERILDSDSV
DVETGCKINNSSTQNSEKKSASAPNVTSNILIIMIVGIRNIILVMTLIVQ
KFRNLFEFWTNCYFACNSLALIIISNPSKLPCLYEWEKVPGLIFLSYFLF
IYYLQHLPSVGIQLIMFQKVLKTMIRVLFSFFTIFFGFAFGFYIINQDQD
PFKNLGLAYMKSLAMMTGEFDLVTYFVSGKKNYYVTSLIMISLFVVIMSI
SLFNLIIGLAVGDIGDIASEATMQAYKLKVGYIMEVETNVPTVIQRYLYH
SEICDRPKQHKKRFIVSWLFQNSFIFTKSEFMFNALIWSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005216ion channel activity
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0034220ion transmembrane transport
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane