Leucine rich melanocyte differentiation associated

Overview


Name	Leucine rich melanocyte differentiation associated
Smed ID	SMED30005425
Length (bp)	1568

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Leucine rich melanocyte differentiation associated (SMED30005425) t-SNE clustered cells

Violin plots show distribution of expression levels for Leucine rich melanocyte differentiation associated (SMED30005425) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Leucine rich melanocyte differentiation associated (SMED30005425) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005425

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30005425	SMESG000076354.1	PL030013A10D05	ncbi_smed_ests	PMID:23079596 Forsthoefel et al., 2012
X1 cell	SMED30005425		SmedASXL_074423	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
gut	SMED30005425	SMESG000076354.1	dd_Smed_v4_1865_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30005425	SMESG000076354.1	dd_Smed_v4_1865_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Human
Match: LRMDA (leucine rich melanocyte differentiation associated [Source:HGNC Symbol;Acc:HGNC:23405])

HSP 1 Score: 49.6766 bits (117), Expect = 3.709e-6
Identity = 52/140 (37.14%), Postives = 74/140 (52.86%), Query Frame = 2

Query:  116 KVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            K L +S N  + LE L    +L E+I DNN + +++ +P L +L+ L+LNKN+I DL+ LLD+LAE TP L+ LSLLGN   P+++ S               E  E D                  R+R  +LY+LPNL
Sbjct:   34 KRLDLSFNLLRSLEGLSAFRSLEELILDNNQLGDDLVLPGLPRLHTLTLNKNRITDLENLLDHLAEVTPALEYLSLLGNVACPNELVSL--------------EKDEEDYK----------------RYRCFVLYKLPNL 143

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Human
Match: LRMDA (leucine rich melanocyte differentiation associated [Source:HGNC Symbol;Acc:HGNC:23405])

HSP 1 Score: 48.9062 bits (115), Expect = 5.139e-6
Identity = 51/144 (35.42%), Postives = 75/144 (52.08%), Query Frame = 2

Query:  113 EKVLLISDNQF---KDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            EK L +S N     + LE L    +L E+I DNN + +++ +P L +L+ L+LNKN+I DL+ LLD+LAE TP L+ LSLLGN   P+++ S                                 D ++  R+R  +LY+LPNL
Sbjct:    2 EKYLSLSGNHSSNKRSLEGLSAFRSLEELILDNNQLGDDLVLPGLPRLHTLTLNKNRITDLENLLDHLAEVTPALEYLSLLGNVACPNELVS------------------------------LEKDEEDYKRYRCFVLYKLPNL 115

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Human
Match: LRMDA (leucine rich melanocyte differentiation associated [Source:HGNC Symbol;Acc:HGNC:23405])

HSP 1 Score: 46.9802 bits (110), Expect = 8.078e-6
Identity = 48/130 (36.92%), Postives = 69/130 (53.08%), Query Frame = 2

Query:  146 KDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            + LE L    +L E+I DNN + +++ +P L +L+ L+LNKN+I DL+ LLD+LAE TP L+ LSLLGN   P+++ S               E  E D                  R+R  +LY+LPNL
Sbjct:    1 RSLEGLSAFRSLEELILDNNQLGDDLVLPGLPRLHTLTLNKNRITDLENLLDHLAEVTPALEYLSLLGNVACPNELVSL--------------EKDEEDYK----------------RYRCFVLYKLPNL 100

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Zebrafish
Match: lrmda (leucine rich melanocyte differentiation associated [Source:ZFIN;Acc:ZDB-GENE-050522-285])

HSP 1 Score: 51.2174 bits (121), Expect = 7.559e-7
Identity = 36/84 (42.86%), Postives = 57/84 (67.86%), Query Frame = 2

Query:  128 ISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYS 379
            +S NQ + L  L     L E+I DNN +  ++++P+L +L+ L+LNKN++ D++ LL++L E TP L+ LSLLGN   P+Q+ S
Sbjct:   42 LSFNQLRSLTGLKAFSQLEELIVDNNLLGNDLRLPRLPRLHTLTLNKNQLTDIEALLEHLVEVTPALEYLSLLGNEACPNQLVS 125

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Mouse
Match: Lrmda (leucine rich melanocyte differentiation associated [Source:MGI Symbol;Acc:MGI:1923883])

HSP 1 Score: 49.6766 bits (117), Expect = 1.349e-6
Identity = 40/86 (46.51%), Postives = 59/86 (68.60%), Query Frame = 2

Query:  116 KVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQI 373
            K L +S N  + LE L    +L E+I DNN + +++ +P L  L+ L+LNKN+I DL+ LLD+LAE TP+L+ LSLLGN   P+++
Sbjct:   34 KRLDLSFNLLRSLEGLSAFRSLEELILDNNLLGDDLVLPGLPHLHTLTLNKNQITDLEYLLDHLAEVTPSLEYLSLLGNVACPNEL 119

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Mouse
Match: Lrmda (leucine rich melanocyte differentiation associated [Source:MGI Symbol;Acc:MGI:1923883])

HSP 1 Score: 50.0618 bits (118), Expect = 1.521e-6
Identity = 40/86 (46.51%), Postives = 59/86 (68.60%), Query Frame = 2

Query:  116 KVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQI 373
            K L +S N  + LE L    +L E+I DNN + +++ +P L  L+ L+LNKN+I DL+ LLD+LAE TP+L+ LSLLGN   P+++
Sbjct:   34 KRLDLSFNLLRSLEGLSAFRSLEELILDNNLLGDDLVLPGLPHLHTLTLNKNQITDLEYLLDHLAEVTPSLEYLSLLGNVACPNEL 119

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Mouse
Match: Lrmda (leucine rich melanocyte differentiation associated [Source:MGI Symbol;Acc:MGI:1923883])

HSP 1 Score: 49.6766 bits (117), Expect = 2.599e-6
Identity = 40/86 (46.51%), Postives = 59/86 (68.60%), Query Frame = 2

Query:  116 KVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQI 373
            K L +S N  + LE L    +L E+I DNN + +++ +P L  L+ L+LNKN+I DL+ LLD+LAE TP+L+ LSLLGN   P+++
Sbjct:   34 KRLDLSFNLLRSLEGLSAFRSLEELILDNNLLGDDLVLPGLPHLHTLTLNKNQITDLEYLLDHLAEVTPSLEYLSLLGNVACPNEL 119

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Match: A0A267GTT8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006051g1 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.500e-10
Identity = 60/158 (37.97%), Postives = 90/158 (56.96%), Query Frame = 2

Query:   62 FAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            F  + L  L P++      +L IS N F+ L+SL  L  L  +IAD+N +   +++P L  L  LSLNKNKI+DL+ L   L    P L+ LSL+GNP +PD I+ H+SRL+    +   + D  +DS+        +FD D+  R+RL + + LP+L
Sbjct:    7 FCWQDLSQLPPDA-DAAVTILDISHNHFRSLDSLAPLKQLKVLIADDNELDSQLRLPDLPHLETLSLNKNKISDLEQLCSELGRRAPRLEFLSLMGNPAAPDAIFQHISRLADAAAA-ADDADDFVDSA------TPDFDEDDFARYRLLVAHLLPSL 156

HSP 2 Score: 29.261 bits (64), Expect = 1.500e-10
Identity = 17/39 (43.59%), Postives = 25/39 (64.10%), Query Frame = 1

Query:  517 LPAAEFALIDSLKVTDIERTEAKRRGQFLQIKRLDPSEN 633
            LP+  +  +DS  VTD ER EA++RG++L   RL  S+ 
Sbjct:  153 LPSLRY--LDSSPVTDRERLEARQRGRYLLKVRLAESDG 189

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Match: A0A267GIX2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023296g1 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.563e-10
Identity = 60/158 (37.97%), Postives = 90/158 (56.96%), Query Frame = 2

Query:   62 FAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            F  + L  L P++      +L IS N F+ L+SL  L  L  +IAD+N +   +++P L  L  LSLNKNKI+DL+ L   L    P L+ LSL+GNP +PD I+ H+SRL+    +   + D  +DS+        +FD D+  R+RL + + LP+L
Sbjct:    6 FCWQDLSQLPPDA-DAAVTILDISHNHFRSLDSLAPLKQLKVLIADDNELDSQLRLPDLPHLETLSLNKNKISDLEQLCSELGRRAPRLEFLSLMGNPAAPDAIFQHISRLADAAAA-ADDADDFVDSA------TPDFDEDDFARYRLLVAHLLPSL 155

HSP 2 Score: 28.8758 bits (63), Expect = 1.563e-10
Identity = 17/39 (43.59%), Postives = 25/39 (64.10%), Query Frame = 1

Query:  517 LPAAEFALIDSLKVTDIERTEAKRRGQFLQIKRLDPSEN 633
            LP+  +  +DS  VTD ER EA++RG++L   RL  S+ 
Sbjct:  152 LPSLRY--LDSSLVTDRERLEARQRGRYLLKVRLAESDG 188

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Match: A0A1I8GPF3 (Ig-like domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 7.378e-7
Identity = 46/130 (35.38%), Postives = 74/130 (56.92%), Query Frame = 2

Query:  152 LESLHFLPNLIEIIADNNYITENIKIP--XXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            L+SL  L  L  +IAD+N +   +++P    L+  +L+ NK  I+DL+ L   L    P L+ LSL+GNP +PD I+ H+SRL+    +   + D  +DS+        +FD D+  R+RL + + LP+L
Sbjct:    2 LDSLAPLKQLKVLIADDNELDSQLRLPDLPHLETLSLNKNKASISDLEQLCSELGRRAPRLEFLSLMGNPAAPDAIFQHISRLADAAAA-ADDADDFVDSA------TPDFDEDDFARYRLLVAHLLPSL 124

HSP 2 Score: 29.6462 bits (65), Expect = 7.378e-7
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = 1

Query:  541 IDSLKVTDIERTEAKRRGQFLQIKRLDPSEN 633
            +DS  VTD ER EA++RG++L   RL  S+ 
Sbjct:  127 LDSSPVTDRERLEARQRGRYLLKVRLAESDG 157

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Match: A0A2C9JFC0 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106055708 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.916e-6
Identity = 33/80 (41.25%), Postives = 54/80 (67.50%), Query Frame = 2

Query:  128 ISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPD 367
            IS N+FKDL  L  LP L  ++AD+N++T ++KIP    L  L +N+N+I +L + +  +A++ PNLK+LS++ N  +P 
Sbjct:   39 ISYNKFKDLRFLQELPKLKSLVADHNHLTSHVKIPHCASLETLWVNQNRIQNLSLFIATVAKSCPNLKILSMMKNEAAPS 118

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Match: A0A2I4AYJ9 (leucine-rich repeat-containing protein C10orf11 homolog isoform X2 OS=Austrofundulus limnaeus OX=52670 GN=LOC106515339 PE=4 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 4.369e-6
Identity = 48/140 (34.29%), Postives = 75/140 (53.57%), Query Frame = 2

Query:  116 KVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNL 535
            K L +S NQ + L  L     L E+I DNN +  ++++P+L  L+ L+LNKN++ D++VLL++LA+ TP+L+ LSLLGN   P+Q+ S                               + D D+  R+R  +LY+LP L
Sbjct:   38 KRLDLSFNQLRSLAGLKMFTELEELIVDNNQLGNDLQLPRLCNLHTLTLNKNQLTDIEVLLEHLADLTPSLQYLSLLGNEACPNQLVS------------------------------PDKDEDDYQRYRYFVLYKLPQL 147

HSP 2 Score: 26.5646 bits (57), Expect = 4.369e-6
Identity = 14/31 (45.16%), Postives = 20/31 (64.52%), Query Frame = 1

Query:  517 LPAAEFALIDSLKVTDIERTEAKRRGQFLQI 609
            LP  +F  +DS KVT  E  EA+ RG F+++
Sbjct:  144 LPQLKF--LDSRKVTKEELMEAQARGAFMKV 172

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Medaka
Match: lrmda (leucine rich melanocyte differentiation associated [Source:NCBI gene;Acc:101174451])

HSP 1 Score: 53.5286 bits (127), Expect = 1.012e-7
Identity = 36/84 (42.86%), Postives = 58/84 (69.05%), Query Frame = 2

Query:  128 ISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYS 379
            +S NQ + L  L     L E++ DNN +  ++++P+L  L+ L+LNKN++ D++ LL++LA+ TP+LK LSLLGN   P+Q+ S
Sbjct:   42 LSFNQLRSLAGLKMFTELEELVVDNNLLGNDLQLPRLPNLHTLTLNKNQLTDIEALLEHLADVTPSLKYLSLLGNEACPNQLVS 125

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Medaka
Match: lrmda (leucine rich melanocyte differentiation associated [Source:NCBI gene;Acc:101174451])

HSP 1 Score: 52.7582 bits (125), Expect = 2.081e-7
Identity = 36/84 (42.86%), Postives = 58/84 (69.05%), Query Frame = 2

Query:  128 ISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYS 379
            +S NQ + L  L     L E++ DNN +  ++++P+L  L+ L+LNKN++ D++ LL++LA+ TP+LK LSLLGN   P+Q+ S
Sbjct:   42 LSFNQLRSLAGLKMFTELEELVVDNNLLGNDLQLPRLPNLHTLTLNKNQLTDIEALLEHLADVTPSLKYLSLLGNEACPNQLVS 125

[Close]

BLAST of Leucine rich melanocyte differentiation associated vs. Planmine SMEST
Match: SMESG000076354.1 (SMESG000076354.1)

HSP 1 Score: 278.1 bits (710), Expect = 7.651e-118
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 2

Query:   59 MFAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPXXXXXXXXXXXXXXXXDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNLH 538
            MFAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPKLLKLNALSLNKNKINDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNLH
Sbjct:    1 MFAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNYITENIKIPKLLKLNALSLNKNKINDLDVLLDNLAENTPNLKLLSLLGNPVSPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRLRILYRLPNLH 160

HSP 2 Score: 166.392 bits (420), Expect = 7.651e-118
Identity = 83/88 (94.32%), Postives = 83/88 (94.32%), Query Frame = 1

Query:  517 LPAAEFALIDSLKVTDIERTEAKRRGQFLQIKRLDPSENRSISMKANNIVSLIPGRVTDWISTYNPLKPLQNTENFTPLDDPKQKDSK 780
            LP   F  IDSLKVTDIERTEAKRRGQFLQIKRLDPSENRSISMKANNIVSLIPGRVTDWISTYNPLKPLQNTENFTPLDDPKQKDSK
Sbjct:  156 LPNLHF--IDSLKVTDIERTEAKRRGQFLQIKRLDPSENRSISMKANNIVSLIPGRVTDWISTYNPLKPLQNTENFTPLDDPKQKDSK 241

The following BLAST results are available for this feature:

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Human
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Celegans
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Fly
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Zebrafish
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Xenopus
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Mouse
BLAST of Leucine rich melanocyte differentiation associated vs. UniProt/SwissProt
BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Cavefish
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Sea Lamprey
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Yeast
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Nematostella
BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Medaka
BLAST of Leucine rich melanocyte differentiation associated vs. Planmine SMEST

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
LRMDA	3.709e-6	37.14	leucine rich melanocyte differentiation associated...	[more]
LRMDA	5.139e-6	35.42	leucine rich melanocyte differentiation associated...	[more]
LRMDA	8.078e-6	36.92	leucine rich melanocyte differentiation associated...	[more]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
lrmda	7.559e-7	42.86	leucine rich melanocyte differentiation associated...	[more]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Lrmda	1.349e-6	46.51	leucine rich melanocyte differentiation associated...	[more]
Lrmda	1.521e-6	46.51	leucine rich melanocyte differentiation associated...	[more]
Lrmda	2.599e-6	46.51	leucine rich melanocyte differentiation associated...	[more]

BLAST of Leucine rich melanocyte differentiation associated vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267GTT8	1.500e-10	37.97	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A267GIX2	1.563e-10	37.97	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1I8GPF3	7.378e-7	35.38	Ig-like domain-containing protein OS=Macrostomum l...	[more]
A0A2C9JFC0	3.916e-6	41.25	Uncharacterized protein OS=Biomphalaria glabrata O...	[more]
A0A2I4AYJ9	4.369e-6	34.29	leucine-rich repeat-containing protein C10orf11 ho...	[more]

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Leucine rich melanocyte differentiation associated vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
lrmda	1.012e-7	42.86	leucine rich melanocyte differentiation associated...	[more]
lrmda	2.081e-7	42.86	leucine rich melanocyte differentiation associated...	[more]

BLAST of Leucine rich melanocyte differentiation associated vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000076354.1	7.651e-118	100.00	SMESG000076354.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005425 ID=SMED30005425|Name=Leucine rich melanocyte differentiation associated|organism=Schmidtea mediterranea sexual|type=transcript|length=1568bp
CTTGTTTAAATAATGCAAAATCTATCAATTTTATTTAAAATTGAACCTTG
ACGGAAAAATGTTTGCTGGTCGGGGTTTGGAAACTTTGAGTCCGAATTCA
TTAACCAACGATGAAAAGGTTCTACTAATTAGTGATAATCAGTTCAAAGA
TTTGGAATCTTTGCATTTTCTTCCGAATCTGATTGAGATTATTGCGGATA
ACAATTATATTACTGAAAATATTAAAATTCCAAAACTCCTGAAGCTTAAT
GCGTTGTCATTAAATAAAAATAAAATTAATGACCTTGATGTTTTGCTTGA
TAATCTGGCCGAAAATACTCCTAACTTGAAATTATTGAGCTTGCTTGGAA
ATCCTGTGTCACCGGATCAGATTTATTCACATTTATCCAGATTATCAATT
CTCCACGAGAGCAGAGATCAGAATGAAGATCAAGAGATTGATTCCAGCAT
TATTAGAGAAGACGGGATAACTAATTTTGATCTTGATGAATGTCTTAGAT
TTAGACTTCGGATTCTCTACCGGCTGCCGAATTTGCACTCATCGACTCTT
TGAAAGTGACAGACATTGAGCGGACGGAGGCCAAAAGGAGAGGTCAATTT
CTTCAAATAAAAAGATTAGATCCCAGTGAGAACCGAAGTATTTCAATGAA
AGCGAACAATATTGTCTCCCTGATTCCGGGACGAGTGACGGATTGGATTT
CGACATATAACCCATTGAAACCCTTACAAAATACTGAAAATTTTACTCCG
CTAGACGACCCTAAGCAAAAGGATTCCAAAGGTTAGTTTTGTTCTTGATT
AATATAATGATGTAAAATTTGGGAAAACTTATTAAATTGTCATTTACTAA
ACCGTAATTTGAACTTGAAAGGTTCATAACTGGAACCAGCAAATTGCTGG
CTTATTCGGTTTATTTGACATCATTAATACATTTAGATCTCGATTGTAGG
TAAGTCTCATGATATGAAAAGAATGTGTTGAAAATTTATGTTAAATAGAA
AAAAACACTGATCTAGAGCAGAGGTCTCCAATGTTTTAAAGAAGTGAGCC
ACATAAAAACTTATCCTGAGTTCAGCGGGCCATATCAAAGTCATAAGTAT
TTGAGCGGGACATAAAGACTACCACGCGGGCCTTAGTTTGGGGTCGCTGG
TCTAGGAGGTTAAATGTAAATGAAATTCCAAAGGTAAACAGATCAATGAT
CATTCTTACTGATTATTAATACTGGAACACAACGAAAGGATTGACACCTG
CCTATATCATGCTGGATCCTTTAATATGTCTTTTATTATTGGACTACTTC
TTGCCAAAGTGAAAGAGTCCTACTATTAACAATAATTCTTTTTTGATTCC
TAGTCATTCAGTTATTACTAAACCTTCCTGCCAATTGGTGCTGGAATCAT
CTACCGATTTGAACCAAAGTTCCTGAAATAAGTTCCTTGAATCCCTAAGA
ATCTGGAAACAAATTTCCTTGTTCCATGACATCAAATAGCAATCACAAAA
CAAGAAAAAAGAGAAAATATACCAGTAAAATCCTTTATCCTTTGTACCAT
TGATCTATTAATCTCTTA

protein sequence of SMED30005425-orf-1

>SMED30005425-orf-1 ID=SMED30005425-orf-1|Name=SMED30005425-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp
MFAGRGLETLSPNSLTNDEKVLLISDNQFKDLESLHFLPNLIEIIADNNY
ITENIKIPKLLKLNALSLNKNKINDLDVLLDNLAENTPNLKLLSLLGNPV
SPDQIYSHLSRLSILHESRDQNEDQEIDSSIIREDGITNFDLDECLRFRL
RILYRLPNLHSSTL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **biological process**
Term	Definition
GO:0043473	pigmentation
GO:0030154	cell differentiation
GO:0030318	melanocyte differentiation

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000070	intestinal phagocyte
PLANA:0002109	X1 cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR032675	LRR_dom_sf

Vocabulary: **molecular function**
Term	Definition
GO:0003674	molecular_function

Vocabulary: **cellular component**
Term	Definition
GO:0005575	cellular_component

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR46282:SF2	LEUCINE-RICH MELANOCYTE DIFFERENTIATION-ASSOCIATED PROTEIN	coord: 21..107
None	No IPR available	PANTHER	PTHR46282	FAMILY NOT NAMED	coord: 21..107
None	No IPR available	SUPERFAMILY	SSF52058	L domain-like	coord: 19..101

Main menu

Leucine rich melanocyte differentiation associated

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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