Tyrosine--tRNA ligase

Overview
NameTyrosine--tRNA ligase
Smed IDSMED30005420
Length (bp)1730
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tyrosine--tRNA ligase (SMED30005420) t-SNE clustered cells

Violin plots show distribution of expression levels for Tyrosine--tRNA ligase (SMED30005420) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tyrosine--tRNA ligase (SMED30005420) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tyrosine--tRNA ligase (SMED30005420) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005420

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005420h1SMcG0018768 SmedASXL_011642SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30005420h1SMcG0018768 SmedASXL_011642SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Match: YARS2 (tyrosyl-tRNA synthetase 2 [Source:HGNC Symbol;Acc:HGNC:24249])

HSP 1 Score: 213.772 bits (543), Expect = 4.145e-62
Identity = 121/300 (40.33%), Postives = 176/300 (58.67%), Query Frame = 2 
Query:  188 QTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLD----DQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK 1072
            QT Y GFDPTA SL +G+L  L+ L  + ++GHNVIALVGGATA++GDP+ R  +RE L T  V + + ++   +E +  N Q  +      G+F +L+N  WY K + + F+     HFR   + +++S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN D  SP+ LY   +  P+  +   R L   T L     D ++Q  + E     +++  Q  LA EV KLVHG   L++ K
Sbjct:   74 QTIYCGFDPTADSLHVGHLLALLGLFHLQRAGHNVIALVGGATARLGDPSGRTKEREALETERVRANARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKSPEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSVE--RYLKLFTFLPLPEIDHIMQLHVKE----PERRGPQKRLAAEVTKLVHGREGLDSAK 367
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Match: YARS2 (tyrosyl-tRNA synthetase 2 [Source:HGNC Symbol;Acc:HGNC:24249])

HSP 1 Score: 200.675 bits (509), Expect = 2.583e-59
Identity = 102/241 (42.32%), Postives = 149/241 (61.83%), Query Frame = 2 
Query:  188 QTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVI 907
            QT Y GFDPTA SL +G+L  L+ L  + ++GHNVIALVGGATA++GDP+ R  +RE L T  V + + ++   +E +  N Q  +      G+F +L+N  WY K + + F+     HFR   + +++S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN D  SP+ LY   +  P+  +
Sbjct:   48 QTIYCGFDPTADSLHVGHLLALLGLFHLQRAGHNVIALVGGATARLGDPSGRTKEREALETERVRANARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKSPEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSV 288
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Match: yars-2 (Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Acc:H2KZL6])

HSP 1 Score: 147.902 bits (372), Expect = 1.073e-38
Identity = 105/314 (33.44%), Postives = 161/314 (51.27%), Query Frame = 2 
Query:  197 YLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTM-----RHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE-LVYGLTVPLFV-VDGKKLGKST-GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPEL 1111
            Y GFDPTA+SL IGNL  L+ L+R  Q G   IAL+G  TA IGDP+ + S+R +L   V+   S  +T  I +I   F++   ++E+     ++NN +W  K++   F+ +       +  R + IKN      RLE    ++ +EF Y  MQA+DW  L   YGCR Q+GG DQ+G++ FG   I++  N+    G+  P+     G KLGKS  G   WL+    SP+  Y     + +       LL   +L D + I+  +   + N  +   Q  LA E+ ++VHG   L  A++ T  +F +   +L
Sbjct:   60 YAGFDPTAESLHIGNLLILVNLIRAQQFGIRPIALIGEFTASIGDPSGKKSERGLLAGDVIMHNSRKVTDQICKI---FENAPGSSEKP---IIVNNNDWLGKISLRDFLRECKNMQVGKMLRMNTIKN------RLEV--GLSYTEFSYQTMQAFDWYTLSEKYGCRFQLGGYDQLGHLDFGAHYIKKMMNQAFAAGVCFPILTDSTGAKLGKSEGGGALWLDATKTSPFHFYQFFAQLHDD--KAEELLLLFSLQDIEHIRDVLKNHRSNLGQWIAQRELAAEITRIVHGKEGLEVAMRCTKAMFGAKKADL 357
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Match: yars-2 (Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Acc:H2KZL6])

HSP 1 Score: 94.3597 bits (233), Expect = 5.494e-21
Identity = 77/267 (28.84%), Postives = 125/267 (46.82%), Query Frame = 2 
Query:  476 LLNNMEWYNKLNTLSFITDTM-----RHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE-LVYGLTVPLFV-VDGKKLGKST-GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISID-SANGFITMNQLVEAASDG 1246
            ++NN +W  K++   F+ +       +  R + IKN      RLE    ++ +EF Y  MQA+DW  L   YGCR Q+GG DQ+G++ FG   I++  N+    G+  P+     G KLGKS  G   WL+    SP+  Y     + +       LL   +L D + I+  +   + N  +   Q  LA E+ ++VHG   L  A++ T  +F +   +     LS ++ S ++      +   +ID       TM QL +A+  G
Sbjct:   27 IVNNNDWLGKISLRDFLRECKNMQVGKMLRMNTIKN------RLEVG--LSYTEFSYQTMQAFDWYTLSEKYGCRFQLGGYDQLGHLDFGAHYIKKMMNQAFAAGVCFPILTDSTGAKLGKSEGGGALWLDATKTSPFHFYQFFAQLHDD--KAEELLLLFSLQDIEHIRDVLKNHRSNLGQWIAQRELAAEITRIVHGKEGLEVAMRCTKAMFGAKKAD-----LSGLSRSEVL-----QLFRTTIDLKKENVATMGQLADASRLG 273
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Match: yars-2 (Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Acc:H2KZL6])

HSP 1 Score: 94.3597 bits (233), Expect = 5.494e-21
Identity = 77/267 (28.84%), Postives = 125/267 (46.82%), Query Frame = 2 
Query:  476 LLNNMEWYNKLNTLSFITDTM-----RHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE-LVYGLTVPLFV-VDGKKLGKST-GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISID-SANGFITMNQLVEAASDG 1246
            ++NN +W  K++   F+ +       +  R + IKN      RLE    ++ +EF Y  MQA+DW  L   YGCR Q+GG DQ+G++ FG   I++  N+    G+  P+     G KLGKS  G   WL+    SP+  Y     + +       LL   +L D + I+  +   + N  +   Q  LA E+ ++VHG   L  A++ T  +F +   +     LS ++ S ++      +   +ID       TM QL +A+  G
Sbjct:   27 IVNNNDWLGKISLRDFLRECKNMQVGKMLRMNTIKN------RLEVG--LSYTEFSYQTMQAFDWYTLSEKYGCRFQLGGYDQLGHLDFGAHYIKKMMNQAFAAGVCFPILTDSTGAKLGKSEGGGALWLDATKTSPFHFYQFFAQLHDD--KAEELLLLFSLQDIEHIRDVLKNHRSNLGQWIAQRELAAEITRIVHGKEGLEVAMRCTKAMFGAKKAD-----LSGLSRSEVL-----QLFRTTIDLKKENVATMGQLADASRLG 273
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Match: yars-2 (Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Acc:H2KZL6])

HSP 1 Score: 94.3597 bits (233), Expect = 5.494e-21
Identity = 77/267 (28.84%), Postives = 125/267 (46.82%), Query Frame = 2 
Query:  476 LLNNMEWYNKLNTLSFITDTM-----RHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE-LVYGLTVPLFV-VDGKKLGKST-GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISID-SANGFITMNQLVEAASDG 1246
            ++NN +W  K++   F+ +       +  R + IKN      RLE    ++ +EF Y  MQA+DW  L   YGCR Q+GG DQ+G++ FG   I++  N+    G+  P+     G KLGKS  G   WL+    SP+  Y     + +       LL   +L D + I+  +   + N  +   Q  LA E+ ++VHG   L  A++ T  +F +   +     LS ++ S ++      +   +ID       TM QL +A+  G
Sbjct:   27 IVNNNDWLGKISLRDFLRECKNMQVGKMLRMNTIKN------RLEVG--LSYTEFSYQTMQAFDWYTLSEKYGCRFQLGGYDQLGHLDFGAHYIKKMMNQAFAAGVCFPILTDSTGAKLGKSEGGGALWLDATKTSPFHFYQFFAQLHDD--KAEELLLLFSLQDIEHIRDVLKNHRSNLGQWIAQRELAAEITRIVHGKEGLEVAMRCTKAMFGAKKAD-----LSGLSRSEVL-----QLFRTTIDLKKENVATMGQLADASRLG 273
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Fly
Match: TyrRS-m (gene:FBgn0035064 transcript:FBtr0072429)

HSP 1 Score: 215.312 bits (547), Expect = 3.399e-63
Identity = 121/354 (34.18%), Postives = 195/354 (55.08%), Query Frame = 2 
Query:  107 KFSKTDFFETLIPLSSQKYFQNLLNK-KQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQ----LGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENEL-VYGLTVPLFVV-DGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPELIQQQLSPINES 1144
            + +   FF  + P ++    + L  + +Q+ Y GFDPTA SL +GNL  ++ L+   ++GH  IALVGGAT  IGDP+ R ++R  L   V+ +   +I + + R+  N ++    +++    L    ++NN +WY  L  + F+ +  RHFR   + ++ S + RLES + M+ +EF Y   QAYDW HL R + C  Q+GG DQ GN+  G +LI   E +  V+GLT+PL    +G K GKS G   WL+ +  SP++LY   + +P+S +   +LL   T +    ++Q + E     +K+  Q++LA +V  LVHG   L  A + T  L+  +   L +  LS I ++
Sbjct:   30 ELTDRGFFHGIFPDTAAPRMKQLFTRGQQSIYAGFDPTADSLHVGNLLVIMGLIHCQRAGHRPIALVGGATGLIGDPSGRKTERNQLGETVIETNLKAIEQQLRRVFENHENCLWDSKKQKLPLAPLIIVNNADWYADLQLIDFVANMGRHFRMGSMLSRSSVQSRLESEDGMSFTEFTYQIFQAYDWLHLLRRHNCCFQMGGSDQTGNLMTGHELISRVERKREVFGLTLPLVTTEEGDKFGKSAGNAVWLDGNKTSPFALYQFFLRMPDSEVE--KLLKLFTFIPLPQVEQLMREHTKEPEKRKAQTLLAEDVTLLVHGESGLKQAERVTNALYKGNVEGLAELNLSEIQQT 381
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Match: yars2 (tyrosyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030131-6268])

HSP 1 Score: 222.246 bits (565), Expect = 1.509e-65
Identity = 122/326 (37.42%), Postives = 179/326 (54.91%), Query Frame = 2 
Query:  173 LLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNE-QLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK-STYDLFTSHTPELIQQQLSPINE 1141
            L +  Q+ Y GFDPTA SL  GNL  +I LL    +GH++IAL+GGATAQIGDP+ R ++RE L    V   +  I + + RI  + + Y+  +  +LG   +LNN  WY   + L F ++  R FR   + ++ S + RL+S   M+ +EF Y   QA+D+  L++ +GCR+Q+GG DQ+GN+  G + I     E VYGLTVPL     G KLGK+ G   WLN D  SP+  Y   + +P++ +   R L   T L    ++  + + + +  K+     LA EV KLVHG   L + K  T  L+ S    L Q   + + E
Sbjct:   65 LRSGPQSIYCGFDPTADSLHAGNLLAIIGLLHFRSAGHHIIALLGGATAQIGDPSGRQTERERLSPAAVQENARGILESLHRIFTHHELYFCPDSSRLGRLTVLNNRSWYKDYDLLEFFSEVGRSFRMGTMLSRHSVQTRLKSAEGMSFTEFSYQLFQAFDFYQLHQLHGCRIQLGGTDQLGNIMSGHEFIHRKTGEEVYGLTVPLVTTSMGDKLGKTAGNAVWLNRDKTSPFEFYQYFLRLPDNSVE--RYLKLFTFLSLSEVENLMEQQRKDPGKRIAHKRLAAEVTKLVHGKDGLESAKRCTTALYHSSIEALEQMSDTELQE 388
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: prps2 (phosphoribosyl pyrophosphate synthetase 2 [Source:Xenbase;Acc:XB-GENE-983985])

HSP 1 Score: 233.417 bits (594), Expect = 2.361e-70
Identity = 126/346 (36.42%), Postives = 189/346 (54.62%), Query Frame = 2 
Query:   68 QMLSHIIKRSIFTKFSKTDFFETLIPLSS--QKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTS 1096
            + LSH    +     ++   F+ + P  S   + ++ L +  QT Y GFDPTA SL +GNL  ++ L+   ++GHNVIA++GGAT ++GDP+ R  +RE L    +      I   +ER+ GN ++        GT  +L+N +WY       F+    RHFR   + ++ S + RL S   M+LSEFLYP  QAYD+ +L+R   C +Q+GG DQ+GN+  G   I+    E V+G+TVPL     G KLGKS G   WLN D  SP+ LY   +  P+S +  GR L   T +    I++ + + Q   +++  Q  LA EV K+VHG   L + K  Y++ +S
Sbjct:   21 RRLSH--SAAFLEAAARRGLFKDVFPAESGPTRLYELLGSGPQTAYCGFDPTADSLHVGNLLAVLGLIHFHRAGHNVIAVIGGATGRLGDPSGRGREREALRPEALAGNIRGIRGCLERLFGNCEALVPGGRPGGTVTILDNEQWYRHRGAADFLASVGRHFRMGPMLSRHSAQSRLGSPEGMSLSEFLYPLFQAYDFYYLHRNRNCTIQLGGTDQLGNIMSGHDFIQRVTGEEVFGVTVPLVTNTAGDKLGKSAGNAIWLNRDRTSPFELYQFFVRQPDSAV--GRFLKLFTFIPLPEIERIMEQHQAEPERRLPQKRLAAEVTKIVHGNGGLESAKR-YEMISS 361
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Match: prps2 (phosphoribosyl pyrophosphate synthetase 2 [Source:Xenbase;Acc:XB-GENE-983985])

HSP 1 Score: 232.261 bits (591), Expect = 2.830e-69
Identity = 127/347 (36.60%), Postives = 188/347 (54.18%), Query Frame = 2 
Query:   68 QMLSHIIKRSIFTKFSKTDFFETLIPLSS--QKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK-STYDLFTS 1096
            + LSH    +     ++   F+ + P  S   + ++ L +  QT Y GFDPTA SL +GNL  ++ L+   ++GHNVIA++GGAT ++GDP+ R  +RE L    +      I   +ER+ GN ++        GT  +L+N +WY       F+    RHFR   + ++ S + RL S   M+LSEFLYP  QAYD+ +L+R   C +Q+GG DQ+GN+  G   I+    E V+G+TVPL     G KLGKS G   WLN D  SP+ LY   +  P+S +  GR L   T +    I++ + + Q   +++  Q  LA EV K+VHG   L + K  T  L+ S
Sbjct:   21 RRLSH--SAAFLEAAARRGLFKDVFPAESGPTRLYELLGSGPQTAYCGFDPTADSLHVGNLLAVLGLIHFHRAGHNVIAVIGGATGRLGDPSGRGREREALRPEALAGNIRGIRGCLERLFGNCEALVPGGRPGGTVTILDNEQWYRHRGAADFLASVGRHFRMGPMLSRHSAQSRLGSPEGMSLSEFLYPLFQAYDFYYLHRNRNCTIQLGGTDQLGNIMSGHDFIQRVTGEEVFGVTVPLVTNTAGDKLGKSAGNAIWLNRDRTSPFELYQFFVRQPDSAV--GRFLKLFTFIPLPEIERIMEQHQAEPERRLPQKRLAAEVTKIVHGNGGLESAKRCTQALYQS 363
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Match: Yars2 (tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917370])

HSP 1 Score: 216.083 bits (549), Expect = 3.931e-63
Identity = 131/381 (34.38%), Postives = 200/381 (52.49%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLD----DQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK-STYDLFTSHTPEL-------IQQQLSPINESRLILDSLNSV 1174
            ML     R +F +F      +T +P    +  +   +  QT Y GFDPT  SL +G+L  L+ L    ++GHNVIALVGG+TA +GDP+ R   RE L    V + ++++ + +E +  N    +      G+F +L+N  W+ + + + F+     HFR   + ++ S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN +  SP+ LY   +   +  +   R L   T L     D ++Q  + E     +K+  Q  LA EV KLVHG   L++ K  T  L+ S    L       +++     + S L+LD   SV
Sbjct:   32 MLVAPRARGLFKEFFPESGTKTELPELFDR--RRAGSSPQTVYCGFDPTGDSLHVGHLLTLLGLFHFQRAGHNVIALVGGSTALLGDPSGRTKGREALSAECVRANAHALRRGLEALAANHARLFADGRPWGSFTVLDNAAWFQEQHLVDFLATVGGHFRMGTLLSRLSVQSRLKSPEGMSLAEFFYQVLQAYDFYYLFQHYGCRVQLGGSDQLGNIMSGYEFIHKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNREKTSPFELYQFFVRQQDDSVE--RYLKLFTFLPLPEIDHIMQLHVKE----PEKRVAQKRLAAEVTKLVHGQEGLDSAKRCTQALYHSSIEALEVMSDQELKELFKEASFSELVLDPGTSV 404
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Match: Yars2 (tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917370])

HSP 1 Score: 201.83 bits (512), Expect = 3.217e-59
Identity = 104/291 (35.74%), Postives = 160/291 (54.98%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTL 940
            ML     R +F +F      +T +P    +  +   +  QT Y GFDPT  SL +G+L  L+ L    ++GHNVIALVGG+TA +GDP+ R   RE L    V + ++++ + +E +  N    +      G+F +L+N  W+ + + + F+     HFR   + ++ S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN +  SP+ LY   +   +  +   R  H  T 
Sbjct:   32 MLVAPRARGLFKEFFPESGTKTELPELFDR--RRAGSSPQTVYCGFDPTGDSLHVGHLLTLLGLFHFQRAGHNVIALVGGSTALLGDPSGRTKGREALSAECVRANAHALRRGLEALAANHARLFADGRPWGSFTVLDNAAWFQEQHLVDFLATVGGHFRMGTLLSRLSVQSRLKSPEGMSLAEFFYQVLQAYDFYYLFQHYGCRVQLGGSDQLGNIMSGYEFIHKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNREKTSPFELYQFFVRQQDDSVERDRSHHAATC 320
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Match: Yars2 (tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:MGI Symbol;Acc:MGI:1917370])

HSP 1 Score: 201.83 bits (512), Expect = 3.217e-59
Identity = 104/291 (35.74%), Postives = 160/291 (54.98%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTL 940
            ML     R +F +F      +T +P    +  +   +  QT Y GFDPT  SL +G+L  L+ L    ++GHNVIALVGG+TA +GDP+ R   RE L    V + ++++ + +E +  N    +      G+F +L+N  W+ + + + F+     HFR   + ++ S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN +  SP+ LY   +   +  +   R  H  T 
Sbjct:   32 MLVAPRARGLFKEFFPESGTKTELPELFDR--RRAGSSPQTVYCGFDPTGDSLHVGHLLTLLGLFHFQRAGHNVIALVGGSTALLGDPSGRTKGREALSAECVRANAHALRRGLEALAANHARLFADGRPWGSFTVLDNAAWFQEQHLVDFLATVGGHFRMGTLLSRLSVQSRLKSPEGMSLAEFFYQVLQAYDFYYLFQHYGCRVQLGGSDQLGNIMSGYEFIHKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNREKTSPFELYQFFVRQQDDSVERDRSHHAATC 320
[Close]
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q5I0L3|SYYM_RAT (Tyrosine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Yars2 PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 2.328e-65
Identity = 135/381 (35.43%), Postives = 200/381 (52.49%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLD----DQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK-STYDLFTSHTPEL-------IQQQLSPINESRLILDSLNSV 1174
            ML     R +F +F      +T +P   + + +      QT Y GFDPT  SL +G+L  L+ L    ++GHNVIALVGG+TA +GDP+ R  +RE L    V + + ++ + +E +  N    +      GTF +L+N  W+ K + + F+     HFR   + ++ S + RL+S   M+L+EF Y  +QAYD+ +L+R YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN +  SP+ LY   I   +  +   R L   T L     D ++Q  + E     +K+  Q  LA EV KLVHG   L++ K  T  L+ S    L       +++     + S L+LD   SV
Sbjct:   32 MLVAPRARGLFKEFFPESGTKTELP---ELFDRRRAGSPQTVYCGFDPTGDSLHVGHLLTLLGLFHFQRAGHNVIALVGGSTALLGDPSGRTKEREALSAECVRANARALQRGLETLAANHARLFADGRPWGTFTVLDNAAWFQKQHLMDFLATVGGHFRMGTLLSRLSVQSRLKSPEGMSLAEFFYQVLQAYDFYYLFRHYGCRVQLGGSDQLGNIMSGYEFIHKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNREKTSPFELYQFFIRQQDDSVE--RYLKLFTFLPLPEIDHIMQLHVKE----PEKRIAQKRLAAEVTKLVHGQEGLDSAKRCTQALYHSSIEALEVMSDQELKELFKEASFSELVLDPGTSV 403
[Close]
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q87J85|SYY1_VIBPA (Tyrosine--tRNA ligase 1 OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=tyrS1 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 1.638e-63
Identity = 137/391 (35.04%), Postives = 205/391 (52.43%), Query Frame = 2 
Query:  167 QNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGT-FKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNT-MNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVIL-NAVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITSVKFNGETVT----DLSLPI 1315
            Q LL++ QT Y GFDPTA SL IG+L PLI L R   +GH  +AL+GGAT  IGDP+ + ++R +    +V+ + N ++  I++++         N QL     ++NN +W   +N + F  D  +HF  + + N+ S ++RL+  +  ++ +EF Y  +Q+YD+  L R YGCR+QIGG DQ GN+  G  L R    E V+GLT+PL    DG K GK+ G   WL+P   SPY+ Y   +   ++ +     L   T L  + I    A+ Q ++ K   Q ILA E+ + VHG   L +A + T  LF+ +      QQLS     +L LD L S+     D     I    L  +    R+  S  ++  NGE V+     LS P+
Sbjct:   25 QTLLSQPQTVYCGFDPTAGSLHIGHLVPLIMLKRFQDAGHQAVALIGGATGMIGDPSFKATERSLNSAEIVSGWVNDLSNQIQQLM---------NHQLSKPMIMVNNADWMRAINVIDFFRDVGKHFSINTMINRESVKQRLQRPDQGISFTEFSYALLQSYDFAELNRQYGCRLQIGGNDQWGNIVSGIDLTRRQNGEQVFGLTLPLITKSDGTKFGKTEGGAVWLDPSKTSPYAFYQFWLGAEDADVY--HFLRYYTFLSCEEIASIEAQDQASQGKPQAQRILAEEMTRFVHGEEGLASAERITQALFSGNV-----QQLSLGELKQLELDGLPSIESAQQDLVELLIESG-LASSKRVAREHISNNAISVNGEKVSADNPSLSFPL 398
[Close]
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q8BYL4|SYYM_MOUSE (Tyrosine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Yars2 PE=1 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 2.749e-62
Identity = 131/381 (34.38%), Postives = 200/381 (52.49%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLD----DQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK-STYDLFTSHTPEL-------IQQQLSPINESRLILDSLNSV 1174
            ML     R +F +F      +T +P    +  +   +  QT Y GFDPT  SL +G+L  L+ L    ++GHNVIALVGG+TA +GDP+ R   RE L    V + ++++ + +E +  N    +      G+F +L+N  W+ + + + F+     HFR   + ++ S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN +  SP+ LY   +   +  +   R L   T L     D ++Q  + E     +K+  Q  LA EV KLVHG   L++ K  T  L+ S    L       +++     + S L+LD   SV
Sbjct:   32 MLVAPRARGLFKEFFPESGTKTELPELFDR--RRAGSSPQTVYCGFDPTGDSLHVGHLLTLLGLFHFQRAGHNVIALVGGSTALLGDPSGRTKGREALSAECVRANAHALRRGLEALAANHARLFADGRPWGSFTVLDNAAWFQEQHLVDFLATVGGHFRMGTLLSRLSVQSRLKSPEGMSLAEFFYQVLQAYDFYYLFQHYGCRVQLGGSDQLGNIMSGYEFIHKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNREKTSPFELYQFFVRQQDDSVE--RYLKLFTFLPLPEIDHIMQLHVKE----PEKRVAQKRLAAEVTKLVHGQEGLDSAKRCTQALYHSSIEALEVMSDQELKELFKEASFSELVLDPGTSV 404
[Close]
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q9W107|SYYM_DROME (Tyrosine--tRNA ligase, mitochondrial OS=Drosophila melanogaster OX=7227 GN=TyrRS-m PE=2 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 3.407e-62
Identity = 121/354 (34.18%), Postives = 195/354 (55.08%), Query Frame = 2 
Query:  107 KFSKTDFFETLIPLSSQKYFQNLLNK-KQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQ----LGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENEL-VYGLTVPLFVV-DGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILN-AVKSTYDLFTSHTPELIQQQLSPINES 1144
            + +   FF  + P ++    + L  + +Q+ Y GFDPTA SL +GNL  ++ L+   ++GH  IALVGGAT  IGDP+ R ++R  L   V+ +   +I + + R+  N ++    +++    L    ++NN +WY  L  + F+ +  RHFR   + ++ S + RLES + M+ +EF Y   QAYDW HL R + C  Q+GG DQ GN+  G +LI   E +  V+GLT+PL    +G K GKS G   WL+ +  SP++LY   + +P+S +   +LL   T +    ++Q + E     +K+  Q++LA +V  LVHG   L  A + T  L+  +   L +  LS I ++
Sbjct:   30 ELTDRGFFHGIFPDTAAPRMKQLFTRGQQSIYAGFDPTADSLHVGNLLVIMGLIHCQRAGHRPIALVGGATGLIGDPSGRKTERNQLGETVIETNLKAIEQQLRRVFENHENCLWDSKKQKLPLAPLIIVNNADWYADLQLIDFVANMGRHFRMGSMLSRSSVQSRLESEDGMSFTEFTYQIFQAYDWLHLLRRHNCCFQMGGSDQTGNLMTGHELISRVERKREVFGLTLPLVTTEEGDKFGKSAGNAVWLDGNKTSPFALYQFFLRMPDSEVE--KLLKLFTFIPLPQVEQLMREHTKEPEKRKAQTLLAEDVTLLVHGESGLKQAERVTNALYKGNVEGLAELNLSEIQQT 381
[Close]
BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Match: sp|Q9Y2Z4|SYYM_HUMAN (Tyrosine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=YARS2 PE=1 SV=2)

HSP 1 Score: 213.772 bits (543), Expect = 1.990e-61
Identity = 121/300 (40.33%), Postives = 176/300 (58.67%), Query Frame = 2 
Query:  188 QTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLD----DQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK 1072
            QT Y GFDPTA SL +G+L  L+ L  + ++GHNVIALVGGATA++GDP+ R  +RE L T  V + + ++   +E +  N Q  +      G+F +L+N  WY K + + F+     HFR   + +++S + RL+S   M+L+EF Y  +QAYD+ +L++ YGCRVQ+GG DQ+GN+  G + I +   E V+G+TVPL     G KLGKS G   WLN D  SP+ LY   +  P+  +   R L   T L     D ++Q  + E     +++  Q  LA EV KLVHG   L++ K
Sbjct:   74 QTIYCGFDPTADSLHVGHLLALLGLFHLQRAGHNVIALVGGATARLGDPSGRTKEREALETERVRANARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKSPEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGAKLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSVE--RYLKLFTFLPLPEIDHIMQLHVKE----PERRGPQKRLAAEVTKLVHGREGLDSAK 367
[Close]
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A4W4GMC2 (Tyrosine--tRNA ligase OS=Electrophorus electricus OX=8005 GN=yars2 PE=3 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 1.136e-71
Identity = 133/346 (38.44%), Postives = 193/346 (55.78%), Query Frame = 2 
Query:   92 RSIFTKFSKTDFFETLIPLSSQKYFQNLLNK-KQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTN-EQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQ 1120
            R +FT   +    ++    +SQ     LL    QT Y GFDPTA SL  GNL  +I LL    +GHNVIAL+GGATAQIGDP+ +  +RE L +  V   + SI   + RI  N + Y+ ++ ++LGT  ++NN  WY   N + F++D  RHFR   + ++ S   RL+S   M+++EF Y   QAYD+ +L++ YGC++Q+GG DQ+GN+  G  LI +   E VYGLT+PL     G KLGK+ G   WLN D  SP+  Y   + +P+S +   R L   T L    +++ + + + +  K+     LA EV KLVHG   L + K         +PE + Q
Sbjct:   42 RLLFTMHKRGVIKDSFPEHASQVQLPQLLKTGAQTVYCGFDPTADSLHTGNLLAVIGLLHFRNAGHNVIALIGGATAQIGDPSGKTKERERLSSAAVEGNARSILDSLNRIFTNHELYFCSDPKKLGTISIVNNASWYKDWNVVDFLSDVGRHFRMGTLLSRHSVRSRLKSAEGMSMTEFSYQLFQAYDFFYLHQLYGCKIQLGGTDQLGNLMSGHDLIHKKIGEEVYGLTIPLVTTSVGDKLGKTAGNALWLNWDKTSPFEFYQYFLRLPDSTVE--RYLKLFTFLPLDEVEKVMEQQRQDPGKRSAHKRLAAEVTKLVHGKEGLESAKRCTSALYHSSPEALDQ 385
[Close]
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: I3JQG0 (Tyrosine--tRNA ligase OS=Oreochromis niloticus OX=8128 PE=3 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 4.103e-71
Identity = 127/302 (42.05%), Postives = 183/302 (60.60%), Query Frame = 2 
Query:  173 LLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQ-SYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK 1072
            L +  QT Y GFDPTA SL +GNL  LI LL    +GH+VIA++GGATAQIGDP+ + S+RE L   VV + ++SI + I+RI  N +  ++  +  LGT  +LNN+ WY     + F+++  RHFR   + ++ S + RL+S   M+L+EF Y   QAYD+ HL + YGC++Q+GG DQ+GN+  G + I +   E+VYGLT+PL     G KLGK+ G   WLN D  SP+ LY   +  P++  V GR L   T L    +++ + + + +  K+     LA EV KLVHG   L + K
Sbjct:   71 LQSGAQTVYCGFDPTADSLHVGNLLALIGLLHFRSAGHHVIAVLGGATAQIGDPSGKTSEREQLCADVVEANTHSIRESIQRIFTNHELHFHDGSRPLGTVTVLNNLSWYKNWGVVGFLSEAGRHFRMGTMLSRHSVQSRLKSAEGMSLTEFTYQVFQAYDFHHLNQIYGCKIQLGGTDQLGNLMSGHEYIHKVSGEVVYGLTIPLVTSTVGDKLGKTAGNAVWLNRDKTSPFELYQFFLRQPDAS-VEGRYLKLFTFLPLAEVEKLMEQQREDPSKRLAHKRLAAEVTKLVHGKEGLESAK 371
[Close]
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A267DZC3 (Tyrosine--tRNA ligase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032306g1 PE=3 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 7.524e-71
Identity = 139/353 (39.38%), Postives = 195/353 (55.24%), Query Frame = 2 
Query:  128 FETLIPLSSQKYFQNLLNKK---QTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTN-EQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE--LVYGLTVPLFVV-DGKKLGKST--GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKS-TYDLFTSHTPELIQQQLSPINESRLIL 1156
            F+ ++PL S+KYF   LN     + FY GFDPTA SL +GNL  ++ LLR+   GH  I +VGGATA+IGDP+ R+SDR  L    V   S SI + +  I  N   Y+  +  + G   +LNN  W+  L+   F++  +   R HD+  K S + RL S + M++ EFLYP  QAYDW HLY+ YGC VQIGG DQ GN+  G   +R +E    L +GLTVPL    DG+KLGKS       WL+ +  SP++LY  L   P++ +  GRLL   T L D+ + +         ++   Q+ LA+ V  L+HG   L A +  T  LF   +  L    L+P + +  +L
Sbjct:   35 FQAVLPLKSEKYFNEYLNSASGGRYFYCGFDPTANSLHLGNLLAIVGLLRLRSLGHGAIFVVGGATARIGDPSGRMSDRVRLAERDVEENSASIGRQLAGIADNHGKYFCCDGARPGEAVVLNNQSWHANLSATEFLSGQLSGARLHDLLGKHSVQTRLNSKHGMSVKEFLYPLFQAYDWLHLYKTYGCLVQIGGNDQTGNIDTGLTCLRRSEQSGVLAFGLTVPLLTTSDGQKLGKSDQLADTVWLSSERTSPFALYQRLRNTPDADV--GRLLRLFTFLSDRQVAELCERHAAAPERYEAQTTLADGVTLLLHGEAGLAAARKWTSILFGEDSAALA--SLTPADLAAGVL 383
[Close]
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A1I8IXQ3 (Tyrosine--tRNA ligase OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.417e-70
Identity = 139/353 (39.38%), Postives = 195/353 (55.24%), Query Frame = 2 
Query:  128 FETLIPLSSQKYFQNLLNKK---QTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTN-EQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENE--LVYGLTVPLFVV-DGKKLGKST--GAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKS-TYDLFTSHTPELIQQQLSPINESRLIL 1156
            F+ ++PL S+KYF   LN     + FY GFDPTA SL +GNL  ++ LLR+   GH  I +VGGATA+IGDP+ R+SDR  L    V   S SI + +  I  N   Y+  +  + G   +LNN  W+  L+   F++  +   R HD+  K S + RL S + M++ EFLYP  QAYDW HLY+ YGC VQIGG DQ GN+  G   +R +E    L +GLTVPL    DG+KLGKS       WL+ +  SP++LY  L   P++ +  GRLL   T L D+ + +         ++   Q+ LA+ V  L+HG   L A +  T  LF   +  L    L+P + +  +L
Sbjct:   38 FQAVLPLKSEKYFNEYLNSASGGRYFYCGFDPTANSLHLGNLLAIVGLLRLRSLGHGAIFVVGGATARIGDPSGRMSDRVRLAERDVEENSASIGRQLAGIADNHGKYFCCDGARPGEAVVLNNQSWHANLSATEFLSGQLSGARLHDLLGKHSVQTRLNSKHGMSVKEFLYPLFQAYDWLHLYKTYGCLVQIGGNDQTGNIDTGLTCLRRSEQSGVLAFGLTVPLLTTSDGQKLGKSDQLADTVWLSSERTSPFALYQRLRNTPDADV--GRLLRLFTFLSDRQVAELCERHAAAPERYEAQTTLADGVTLLLHGEAGLAAARKWTSILFGEDSAALA--SLTPADLAAGVL 386
[Close]
BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Match: A0A3P9P5E9 (Tyrosine--tRNA ligase OS=Poecilia reticulata OX=8081 PE=3 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 2.472e-70
Identity = 127/318 (39.94%), Postives = 185/318 (58.18%), Query Frame = 2 
Query:  173 LLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQS-YYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQ 1120
            L +  QT Y GFDPTA SL +GNL  +I LL    +GHNV+AL+GGATAQIGDP+ + S+RE L   VV   +  I + I+RI  N +  ++  +  LGT  +LNN  WY     + F++ T RHFR   + ++ S + RL S + M+L+EF Y   QAYD+ HL++ YGCR+Q+GG DQ+GN+  G + I +   E VYGLT+PL     G KLGK+ G   WLN D  SP+ LY   +  P++ +   R L   T L  + +++ + + + +  K+     LA EV KLVHG   L + K   +     + + ++Q
Sbjct:   71 LQSAPQTVYCGFDPTADSLHVGNLLAIIGLLHFRSAGHNVVALLGGATAQIGDPSGKTSERERLSADVVGENTRGIRESIQRIFVNHEVLFHDRSRPLGTVNVLNNRSWYKSWGVVDFLSGTGRHFRMGTMLSRHSVQSRLRSADGMSLTEFTYQVFQAYDFYHLHQIYGCRIQLGGTDQLGNLMSGHEYIHKVSGEEVYGLTIPLVTSTTGDKLGKTAGNAVWLNRDKTSPFELYQFFLRQPDASVE--RYLKLFTFLSLEEVEKLMEQQREDPSKRLAHKRLAAEVTKLVHGKEGLESAKRCTNALYHSSIQALEQ 386
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Cavefish
Match: yars2 (tyrosyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030131-6268])

HSP 1 Score: 232.261 bits (591), Expect = 4.526e-69
Identity = 128/352 (36.36%), Postives = 193/352 (54.83%), Query Frame = 2 
Query:   77 SHIIKRSIFTKFSKTDFFETLIPLSS-QKYFQNLL-NKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYV-TNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQ 1120
            S   + S+ +   +    +   P S+ Q     LL +  QT Y GFDPTA SL  GNL  LI LL    +GHNV+A+VGGATAQ+GDP+ ++ +RE L +      + SIT  + RI  N +  +   + +LGT  +LNN  WY     + F+++  R+FR   + ++ S + RL+S   M+L+EF Y   QAYD+ HL++ +GCR+Q+GG DQ+GN+  G   I +   E VYGLTVPL     G KLGK+ G   WLN D  SP+  Y   + +P+S + +   +   T L    +++ + + + +  K+     LA EV KLVHG   L   K   +   + + E ++Q
Sbjct:   40 SAAARDSLLSALHRRGVLKDCFPESAAQHELPRLLQSGPQTVYCGFDPTADSLHAGNLLALIGLLHFRSAGHNVLAVVGGATAQVGDPSGKLRERERLSSAAAEHNTRSITDSLWRIFTNHELLFCPESARLGTVSVLNNAGWYRDWRVVDFLSEVGRYFRMGTLLSRHSVQTRLKSAEGMSLTEFSYQLFQAYDFYHLHQLHGCRLQLGGSDQLGNLMTGHDFIHKKTGEEVYGLTVPLVTTTMGDKLGKTAGNAVWLNRDKTSPFEFYQYFLRLPDSTVES--YIKLFTFLPLAEVEKVMEQQRRDPGKRTAHKRLAAEVTKLVHGKDGLETAKRCTNALYNSSIEALEQ 389
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Sea Lamprey
Match: yars2 (tyrosyl-tRNA synthetase 2, mitochondrial [Source:ZFIN;Acc:ZDB-GENE-030131-6268])

HSP 1 Score: 234.572 bits (597), Expect = 7.846e-71
Identity = 144/432 (33.33%), Postives = 217/432 (50.23%), Query Frame = 2 
Query:  101 FTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYY---------VTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFV-VDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVIL-NAVKSTYDLFTSHTPELIQQQLSPINE-------SRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITSVKFNGETVTDLSLPIKSSILKQRI 1342
            F           + P +S +    L + +Q  Y GFDPTA SL +G+L  L+ +L   ++GH+ +ALVGGATA +GDP+ R  +RE L    V   +  I   + R+  N ++               QLG F +LNN  WY + + + F++   RHFR   + ++ S + RL+S   M+L+EF Y   QAYD+ HL+R  GC VQ+GG DQ+GN+  GQ+LI +   + VYG+TVPL     G KLGK+ G   WLN D  SP+ LY   I +P++ +   R L  LT L    +   +   +    K+  Q  LA EV+KLVHG   L +A++ T  L+   T +L     + + E       + L+L+   S+  + +          Q V   S G  +     V+ N E VT+   P +  +L Q +
Sbjct:   40 FLDVHARGLLHGVYPEASLELRALLESARQCVYCGFDPTADSLHVGHLLALVLMLHCQRAGHDAVALVGGATALVGDPSGRAGEREPLSAEAVERNAARIGDSLRRVASNHEALLWRPDSQGPPGRRRQLGAFAVLNNASWYGERDVVGFLSTVGRHFRMGTMLSRHSVQSRLKSPEGMSLAEFSYQMFQAYDFLHLHRELGCVVQLGGADQLGNIMSGQELIHKVTGKEVYGITVPLMTSTTGDKLGKTAGNALWLNRDRTSPFDLYQHFIRLPDAEVE--RYLKLLTFLPLAEVSHVMERHRETPDKRGAQRRLATEVVKLVHGQDGLESAIRCTKVLYEGSTQDLEAMDDTELRELFRESSFTELVLEPGTSLHDMCLRV--------QAVPEGSHGLQMIESGGVRVNHERVTN---PDQVLVLGQHV 458
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Yeast
Match: MSY1 (Mitochondrial tyrosyl-tRNA synthetase [Source:SGD;Acc:S000006018])

HSP 1 Score: 177.948 bits (450), Expect = 8.367e-50
Identity = 96/301 (31.89%), Postives = 158/301 (52.49%), Query Frame = 2 
Query:  179 NKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQL------GTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETE----NELVYGLTVPLF-VVDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHG 1048
            N K   Y G DPTA+SL +GNL PL+ LL  +  GH+++ ++GGAT ++GDP+ R ++R+++   +  S   SI++ ++R   N   YY     L      G +   NN  W+  +  L F+ D  RH R   +  + S   RL++ N +  +EF Y  +QAYD+ HLY+     +Q+GG DQ GN+  G  LI   +      L +G+TVPL     G+K GKS G   +++P   + Y +Y       ++ +   + L   T L+   I++ +     +   +Y Q++LA EV  +++G
Sbjct:   83 NDKIKLYCGVDPTAQSLHLGNLVPLMVLLHFYVKGHDIVTVIGGATGKVGDPSGRKTERDVMENDIRQSNVASISQQLQRFFKNGLEYYRNRCALTEDVPSGKYTPRNNFNWWKDIKMLDFLADFGRHIRVQSMLARDSISSRLQTKNGLGFNEFTYQVLQAYDFYHLYKEENVTIQVGGNDQWGNITAGIDLINRIQPIKNKGLPFGITVPLLTTATGEKFGKSAGNAVFIDPSINTAYDVYQFFYNTLDADVP--KFLKIFTFLNSSEIKKIVETHIKSPSLRYGQTLLAKEVTDMLYG 381
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Nematostella
Match: EDO25499 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DWB0])

HSP 1 Score: 130.183 bits (326), Expect = 8.297e-35
Identity = 69/202 (34.16%), Postives = 114/202 (56.44%), Query Frame = 2 
Query:  146 LSSQKYFQNLLNKKQ-TFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNT-MNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIR 745
            ++S++  Q  L +   T Y GFDPTA SL IG+L PL+ L R    GH  +ALVGGAT  IGDP+ +  +R++     V  +       +E++     ++   +    + +++NN++W   +N LSF+ D  +HF  + +  K S ++R++     ++ +EF Y  +Q+YD+Q L   + C +QIGG DQ GN+  G +L R
Sbjct:   19 MTSEEELQAHLEQGSVTLYCGFDPTADSLHIGSLVPLLTLRRFQNYGHKPLALVGGATGLIGDPSFKAQERKLNDDATVAGW-------VEKLKAQVSAFISFDSGANSAEVVNNLDWTQDMNILSFLRDVGKHFSVNQMIAKESVKQRIDREGEGISFTEFTYMILQSYDFQQLNLSHRCTLQIGGSDQWGNITGGTELTR 213
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Medaka
Match: yars2 (tyrosyl-tRNA synthetase 2 [Source:NCBI gene;Acc:101166222])

HSP 1 Score: 228.409 bits (581), Expect = 6.731e-68
Identity = 122/304 (40.13%), Postives = 177/304 (58.22%), Query Frame = 2 
Query:  167 QNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYV-TNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK 1072
            Q L +  QT Y GFDPTA SL +GNL  +I LL    +GH+VIA++GGATAQIGDP+ + S+RE L    V + + +I + I+RI  N + Y+  +   LGT  +LNNM WY     + F+++  RH R   + ++ S + RL+S + M+L+EF Y   QA+D+ HL +  GCR+Q+GG DQ+GN+  G + I++   E VYGLT+PL     G KLGK+ G   WLN D  SP+ LY   +  P++ +     L   T L    ++  +   + +  K+     LA EV KLVHG   L + K
Sbjct:   62 QLLQSTPQTVYCGFDPTADSLHVGNLLAIIGLLHFRGAGHHVIAVLGGATAQIGDPSGKTSERERLSVTSVEANTQTIRESIQRIFTNHEMYFHDSGRTLGTATVLNNMSWYKSWGVVEFLSEAGRHLRMGTMLSRHSVQARLKSPDGMSLTEFTYQVFQAFDFHHLNQTRGCRIQLGGTDQLGNLMSGHEYIQKVSGEDVYGLTIPLVTNSMGDKLGKTAGNAVWLNRDKTSPFELYQFFLRQPDANVEG--YLKLFTFLPLAEVESLMKLQREDPGKRLAHKRLAAEVTKLVHGKEGLESAK 363
[Close]
BLAST of Tyrosine--tRNA ligase vs. Ensembl Medaka
Match: yars2 (tyrosyl-tRNA synthetase 2 [Source:NCBI gene;Acc:101166222])

HSP 1 Score: 228.409 bits (581), Expect = 6.731e-68
Identity = 122/304 (40.13%), Postives = 177/304 (58.22%), Query Frame = 2 
Query:  167 QNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYV-TNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVD-GKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVK 1072
            Q L +  QT Y GFDPTA SL +GNL  +I LL    +GH+VIA++GGATAQIGDP+ + S+RE L    V + + +I + I+RI  N + Y+  +   LGT  +LNNM WY     + F+++  RH R   + ++ S + RL+S + M+L+EF Y   QA+D+ HL +  GCR+Q+GG DQ+GN+  G + I++   E VYGLT+PL     G KLGK+ G   WLN D  SP+ LY   +  P++ +     L   T L    ++  +   + +  K+     LA EV KLVHG   L + K
Sbjct:   62 QLLQSTPQTVYCGFDPTADSLHVGNLLAIIGLLHFRGAGHHVIAVLGGATAQIGDPSGKTSERERLSVTSVEANTQTIRESIQRIFTNHEMYFHDSGRTLGTATVLNNMSWYKSWGVVEFLSEAGRHLRMGTMLSRHSVQARLKSPDGMSLTEFTYQVFQAFDFHHLNQTRGCRIQLGGTDQLGNLMSGHEYIQKVSGEDVYGLTIPLVTNSMGDKLGKTAGNAVWLNRDKTSPFELYQFFLRQPDANVEG--YLKLFTFLPLAEVESLMKLQREDPGKRLAHKRLAAEVTKLVHGKEGLESAK 363
[Close]
BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Match: SMESG000025871.1 (SMESG000025871.1)

HSP 1 Score: 882.093 bits (2278), Expect = 0.000e+0
Identity = 429/430 (99.77%), Postives = 430/430 (100.00%), Query Frame = 2 
Query:   71 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITSVKFNGETVTDLSLPIKSSILKQRICGVLQI 1360
            MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNN+EWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITSVKFNGETVTDLSLPIKSSILKQRICGVLQI
Sbjct:    1 MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTAKSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPTLVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNLEWYNKLNTLSFITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYRCYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVDGKKLGKSTGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAIAEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQQLSPINESRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITSVKFNGETVTDLSLPIKSSILKQRICGVLQI 430
The following BLAST results are available for this feature:
BLAST of Tyrosine--tRNA ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
YARS24.145e-6240.33tyrosyl-tRNA synthetase 2 [Source:HGNC Symbol;Acc:... [more]
YARS22.583e-5942.32tyrosyl-tRNA synthetase 2 [Source:HGNC Symbol;Acc:... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
yars-21.073e-3833.44Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Ac... [more]
yars-25.494e-2128.84Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Ac... [more]
yars-25.494e-2128.84Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Ac... [more]
yars-25.494e-2128.84Tyrosine--tRNA ligase [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TyrRS-m3.399e-6334.18gene:FBgn0035064 transcript:FBtr0072429[more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
yars21.509e-6537.42tyrosyl-tRNA synthetase 2, mitochondrial [Source:Z... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
prps22.361e-7036.42phosphoribosyl pyrophosphate synthetase 2 [Source:... [more]
prps22.830e-6936.60phosphoribosyl pyrophosphate synthetase 2 [Source:... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Yars23.931e-6334.38tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:... [more]
Yars23.217e-5935.74tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:... [more]
Yars23.217e-5935.74tyrosyl-tRNA synthetase 2 (mitochondrial) [Source:... [more]
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BLAST of Tyrosine--tRNA ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5I0L3|SYYM_RAT2.328e-6535.43Tyrosine--tRNA ligase, mitochondrial OS=Rattus nor... [more]
sp|Q87J85|SYY1_VIBPA1.638e-6335.04Tyrosine--tRNA ligase 1 OS=Vibrio parahaemolyticus... [more]
sp|Q8BYL4|SYYM_MOUSE2.749e-6234.38Tyrosine--tRNA ligase, mitochondrial OS=Mus muscul... [more]
sp|Q9W107|SYYM_DROME3.407e-6234.18Tyrosine--tRNA ligase, mitochondrial OS=Drosophila... [more]
sp|Q9Y2Z4|SYYM_HUMAN1.990e-6140.33Tyrosine--tRNA ligase, mitochondrial OS=Homo sapie... [more]
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BLAST of Tyrosine--tRNA ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4W4GMC21.136e-7138.44Tyrosine--tRNA ligase OS=Electrophorus electricus ... [more]
I3JQG04.103e-7142.05Tyrosine--tRNA ligase OS=Oreochromis niloticus OX=... [more]
A0A267DZC37.524e-7139.38Tyrosine--tRNA ligase (Fragment) OS=Macrostomum li... [more]
A0A1I8IXQ31.417e-7039.38Tyrosine--tRNA ligase OS=Macrostomum lignano OX=28... [more]
A0A3P9P5E92.472e-7039.94Tyrosine--tRNA ligase OS=Poecilia reticulata OX=80... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
yars24.526e-6936.36tyrosyl-tRNA synthetase 2, mitochondrial [Source:Z... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
yars27.846e-7133.33tyrosyl-tRNA synthetase 2, mitochondrial [Source:Z... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MSY18.367e-5031.89Mitochondrial tyrosyl-tRNA synthetase [Source:SGD;... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO254998.297e-3534.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
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BLAST of Tyrosine--tRNA ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
yars26.731e-6840.13tyrosyl-tRNA synthetase 2 [Source:NCBI gene;Acc:10... [more]
yars26.731e-6840.13tyrosyl-tRNA synthetase 2 [Source:NCBI gene;Acc:10... [more]
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BLAST of Tyrosine--tRNA ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000025871.10.000e+099.77SMESG000025871.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005420 ID=SMED30005420|Name=Tyrosine--tRNA ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=1730bp
GTTAAATTTTCAAATATTTAATATTTTTTTTCACCGATAATAAATTACCA
AATTAATTTTAAGGTATCAAATGCTTAGTCACATAATAAAAAGAAGTATA
TTTACAAAATTCAGTAAAACCGATTTTTTCGAAACATTAATACCACTCAG
CAGTCAAAAGTATTTTCAAAACCTATTGAACAAAAAACAAACATTTTATC
TGGGTTTTGACCCAACAGCAAAAAGTTTACAGATTGGGAATCTTTTTCCT
TTGATTGCGCTTTTACGAATTTGGCAATCTGGTCATAATGTTATCGCATT
AGTCGGTGGTGCAACAGCACAAATCGGTGATCCGACTGATCGAGTTAGTG
ATCGAGAGATATTGCCCACTTTAGTTGTAAATTCATTTAGTAATTCAATA
ACAAAAGATATTGAAAGAATCATTGGAAATTTTCAATCTTATTATGTCAC
CAATGAACAACTGGGAACTTTCAAATTGTTGAACAATATGGAATGGTACA
ATAAACTCAATACATTATCTTTCATCACGGACACAATGAGACATTTTCGA
TTTCATGACATAAAGAACAAACGAAGTTTCGAGCGGCGTTTAGAATCGAC
AAATACGATGAACCTGTCGGAATTTCTCTATCCATTCATGCAAGCATACG
ATTGGCAGCATTTGTATCGGTGTTACGGATGCCGAGTGCAGATTGGCGGT
GGCGATCAAATCGGAAACATGCAATTTGGGCAGAAACTCATCAGGGAAAC
CGAGAATGAATTAGTTTACGGTCTGACGGTGCCCCTGTTCGTAGTGGACG
GCAAGAAGCTCGGAAAATCGACCGGTGCGGAATTCTGGCTCAATCCGGAT
GCCCAATCGCCATATTCACTCTATCACAGCCTGATAACAGTCCCGAACTC
GGTGATCGTAACCGGGCGACTGCTGCATCGATTGACGCTGCTTGATGACC
AAGTCATCCAGCAGGCCATTGCAGAGTTCCAGGTGAATAGGAAGAAACAA
TATTTGCAATCGATTCTAGCCAATGAGGTATTGAAGCTGGTTCATGGCCC
TGTCATTCTCAATGCAGTCAAATCGACTTACGATCTTTTTACCTCACACA
CCCCAGAATTGATTCAGCAGCAGTTGTCCCCGATAAATGAGTCGAGACTT
ATTCTCGACAGTCTAAATAGCGTTCCTCACATCTCTATCGACTCTGCAAA
TGGATTTATTACCATGAATCAACTCGTTGAAGCCGCCTCCGATGGCAGAG
ACATTAACTCAATTACCAGCGTTAAATTCAATGGGGAAACTGTTACTGAT
CTCTCGCTACCAATCAAAAGTTCCATTTTAAAACAGCGTATTTGTGGAGT
TTTACAAATCGATTTTTGTCATTCCATTTTTATTTCTCTCGGGGACAAAA
GTTTGATGAAAATGTATAAAGGTTTAATGAAAGACAAGTAAATACTACCA
GAATCGGAAAATGATCTGAAGCGCACGGTGGAACAGTAAAAATGTCGGAA
AATTCTGATATGCAAAATGATTGATAACAATTCAAATCTACAAATTGGTT
CAATCAAAATTAGACAAATTAGAGTAATAGAATATACATAAATAAACTAA
CCAATAAGGGGTCATCCATAAATGAGGTCACGCACCTAGGAGCAGGGGAT
CCCACATTTGTGACAATGGAAGGTGTTTAAGTACTGTGACGTCACATCTT
AACTTTTGAATTTTTAAAATAATTTCAGCT
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protein sequence of SMED30005420-orf-1

>SMED30005420-orf-1 ID=SMED30005420-orf-1|Name=SMED30005420-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=457bp
MLSHIIKRSIFTKFSKTDFFETLIPLSSQKYFQNLLNKKQTFYLGFDPTA
KSLQIGNLFPLIALLRIWQSGHNVIALVGGATAQIGDPTDRVSDREILPT
LVVNSFSNSITKDIERIIGNFQSYYVTNEQLGTFKLLNNMEWYNKLNTLS
FITDTMRHFRFHDIKNKRSFERRLESTNTMNLSEFLYPFMQAYDWQHLYR
CYGCRVQIGGGDQIGNMQFGQKLIRETENELVYGLTVPLFVVDGKKLGKS
TGAEFWLNPDAQSPYSLYHSLITVPNSVIVTGRLLHRLTLLDDQVIQQAI
AEFQVNRKKQYLQSILANEVLKLVHGPVILNAVKSTYDLFTSHTPELIQQ
QLSPINESRLILDSLNSVPHISIDSANGFITMNQLVEAASDGRDINSITS
VKFNGETVTDLSLPIKSSILKQRICGVLQIDFCHSIFISLGDKSLMKMYK
GLMKDK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR024088Tyr-tRNA-ligase_bac-type
IPR002307Tyr-tRNA-ligase
IPR014729Rossmann-like_a/b/a_fold
IPR002305aa-tRNA-synth_Ic
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0005524ATP binding
GO:0004831tyrosine-tRNA ligase activity
GO:0004812aminoacyl-tRNA ligase activity
GO:0003723RNA binding
GO:0016874ligase activity
Vocabulary: biological process
TermDefinition
GO:0006437tyrosyl-tRNA aminoacylation
GO:0006418tRNA aminoacylation for protein translation
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002307Tyrosine-tRNA ligasePRINTSPR01040TRNASYNTHTYRcoord: 179..194
score: 44.38
coord: 52..74
score: 31.16
coord: 233..245
score: 29.49
coord: 200..222
score: 36.38
IPR002307Tyrosine-tRNA ligaseTIGRFAMTIGR00234TIGR00234coord: 29..412
e-value: 8.1E-69
score: 230.6
NoneNo IPR availableGENE3DG3DSA:1.10.240.10coord: 240..348
e-value: 1.2E-17
score: 65.9
NoneNo IPR availableSUPERFAMILYSSF52374Nucleotidylyl transferasecoord: 20..328
IPR002305Aminoacyl-tRNA synthetase, class IcPFAMPF00579tRNA-synt_1bcoord: 38..336
e-value: 3.9E-49
score: 167.6
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 1..239
e-value: 9.9E-74
score: 249.8
IPR024088Tyrosine-tRNA ligase, bacterial-typePANTHERPTHR11766TYROSYL-TRNA SYNTHETASEcoord: 9..412