SMED30005411
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005411 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30005411 aligns in the following genomic locations:
Homology
BLAST of SMED30005411 vs. TrEMBL
Match: A0A0R3X0N7 (Bravo_FIGEY domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS6697 PE=4 SV=2) HSP 1 Score: 103.99 bits (258), Expect = 6.342e-19 Identity = 152/701 (21.68%), Postives = 274/701 (39.09%), Query Frame = 2 Query: 86 PAVKLRKTE----------YTVRNEKLSIDAYLSQQIL--VTLIGKQP--FQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVSGNSNF----LRGFYFCRLENQFDSIVSNNIEIHNIHYYRS--LIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPW------NKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFK--WEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFP--NQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFE------NSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISE---EDNLLKTYLNITNGSLIL-----KRIFSDAWNDVKIACKLTIYAPKVFINHETQYE-KIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEK------TIITVSLISVIYICGIALFLK 2035 P +K RK E Y NE + +D + I+ V LI P +F C L + A+ C++ S C +S F KF D G+I + N + L YFCRL N I SN IE+ ++ Y + + ++S +P + PI + L+ G ++ + +P W Q W C D C+ + ++ N ++ + D V +L + S++ + N + + + + II S + + R G+ L+A YT + + W K SSL +F L FP+ + G+Y L L S + +F + IV P I + P I P NQ + G+ + +L IN I ++S A ++ S+ L + FPY+ + E +SIR ++ L G V+ V + G +R T++ ++ P R ++ C + C H F CD Y ++ W E++G+++ DN L T+L +N ++L +F + + +V ++C+ I+ P+ + YE Y + D + L + P K++L+++ +I V ++ ++ I + + ++ Sbjct: 18 PFLKFRKVESATHRNGSFSYFGTNEPIIVDGVFPRVIIQIVELINNVPPKLEFHCVVDKLSKDSPITSAQLCFETPASRKLNCDNALGSSYLLSDKF-KFKSTGDIYNGYIFTSNWDRDRFPLDATYFCRLSNSNGVIQSNAIEVQDMDSYTTAFIKRQSLEP---ILRGPIVGSSFPMSLECGGYMD--HVELPNWAKQTFPVPYDWVACEAEADS-QLDIRMCYDRSRSIAGYFEHTVLPNGTLIIHDLVKDWRPIGILCTTSIVRNKIFSAYFQDNDGKAVPFTYIPGTYPNQIIPRSPQDNRVALRSGWHARDTITLNAFYTANPKTVTAMWTKDDSSLPIQFTSNRHSLVFPQDIKPTYAGLYVLSLKSPHEQLLFKYEV---IVEQP--PTIKEAPPRHIIIPQGNQAQVIADFSGGLTSR-SLLINGIQIDVVSSARYRESLRLLQESFPYVGDLNPEIEFPSDSSIRYTVRDLAFAPN--------SPYGSSHIVTMVVTNALGSVRTNTLIRVLPKPDVIRQLSDYSCVEDCFNHTTHRFYCDIEVAPYT--GLRVVKTW--ELDGQDLERLDPRDNRL-TFLKHSNQEVVLNIAQDNALFDELFANVDLSCRFRIFGPETDLFAGYMYETPYYDTKNDPALHAILTAHIRKYPS-KSKLVTVASISWIVAVVIAVLILLIVAILAVCIVMR 691
BLAST of SMED30005411 vs. TrEMBL
Match: W6UK84 (Bravo_FIGEY domain-containing protein OS=Echinococcus granulosus OX=6210 GN=EGR_03208 PE=4 SV=1) HSP 1 Score: 102.449 bits (254), Expect = 1.727e-18 Identity = 150/707 (21.22%), Postives = 273/707 (38.61%), Query Frame = 2 Query: 47 SENLERILRFAPNPAVKLRKTEYTV--RNEKLSIDAYLSQQIL--VTLIGKQP--FQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVS---GNSNF-LRGFYFCRLENQFDSIVSNNIEIHNIHYYRS--LIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKF------RIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENF--KWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVK--GMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFE------NSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCD----TFSDQYIKRNFKIDTQWMVEVNGREISEEDNLLKTYLNITNGSLIL-----KRIFSDAWNDVKIACKLTIYAPKVFINHETQYE-KIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEK------TIITVSLISVIYICGIALFLK 2035 EN L+F + LR ++ N+ + ID + + V LI P +F C L + A+ C++ S C +S F KF D G+I + G F L YFCRL N I SN+IE+ ++ Y + + ++S P + PI + L+ G ++ + +P W Q W C D +C+ + ++ +N ++ + D V +L + S++ ++ + + + + + II S + + R G+ L+A YT + KW K SSL +F L FP+ + G Y L L S Y F + V + P I + P I P Q ++V + G +L IN I +S ++ S+ L + FPY+ + E SIR ++ L G V+ V + G +R T++ ++ P R ++ C + C H F CD +S + + +++D Q + ++ R DN L T+L N ++L +F + + +V ++C+ ++ P + YE Y + D + L + P K++L ++ +I V ++ ++ I + + ++ Sbjct: 23 GENGTPFLKFHKVESATLRNGSFSYFGTNKPIVIDGMFPRVTIQIVELINNAPPKLEFHCVVDKLSKDSPITSAQLCFETPFSRKLTCDNALGSSYLLSDKF-KFKSMGDIYNGYIFTSNWGRDKFPLDATYFCRLGNSNGVIQSNSIEVQDMDSYTTAFIKRQSLDP---ILRGPIVGSSFPMSLECGGSMD--HVELPNWAKQTFPVPYDWVACEAEADS-QLEIRKCYDRSQPIAEYFEHTVLSNGTLIIHDLVKDWRPIGILCTTSIVRNKIFSAYFQDNDGKAVPFTYIPGTYPNQIIPRSPQHNRVALRSGWHARDIITLNAFYTANPRTVSAKWTKDDSSLPIQFTSNRHSLVFPQDIKPSYAGTYVLSLKS--PYGQLRFKYEVSVEQPPTIK---EAPPRHLIVP-QGNQAQVVAEFSGRLTSRSLLINGIQIDAVSSVRYRESLRLLQESFPYVGDLNPEIEFPSDTSIRYTVRDLAFAPN--------SPYGSSHIVTMVVTNALGSVRTNTLIRVLPKPDVIRQLSDYSCVEDCFNHTTHRFYCDIEMAPYSGLRVVKTWELDGQDLQRLDPR-----DNRL-TFLKYLNREVVLNIAQDNALFDELFANVDLSCRFRVFGPDTDLFAGYMYETPYYDTKNDPALHAMLTAHIRKYPS-KSKLAAVSSISWIVAVVIAVLILLIVAILAVCIVMR 701
BLAST of SMED30005411 vs. TrEMBL
Match: A0A068YDM3 (Bravo_FIGEY domain-containing protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001058700 PE=4 SV=1) HSP 1 Score: 102.449 bits (254), Expect = 1.886e-18 Identity = 152/710 (21.41%), Postives = 274/710 (38.59%), Query Frame = 2 Query: 38 NFASENLERILRFAPNPAVKLRKTEYTV--RNEKLSIDAYLSQQIL--VTLIGKQP--FQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVSGNSNF----LRGFYFCRLENQFDSIVSNNIEIHNIHYYRS--LIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPW------NKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENF--KWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVK--GMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFE------NSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCD----TFSDQYIKRNFKIDTQWMVEVNGREISEEDNLLKTYLNITNGSLIL-----KRIFSDAWNDVKIACKLTIYAPKVFINHETQYE-KIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEK------TIITVSLISVIYICGIALFLK 2035 N +N L+F + LR ++ N+ + ID Q + V LI P +F C L + A+ C++ S C +S F KF D G+I + N + L YFCRL N I SN+IE+ +I Y + + ++S P + PI + L+ G ++ + +P W Q W C D +C+ + ++ +N ++ + D V +L + S++ + N + + + + II S + + R G+ L+A YT + KW K SSL +F L FP+ + G Y L L S Y F + V + P I + P I P Q ++V + G +L IN I +S ++ S+ L + FPY+ + E SIR ++ L G V+ V + G +R T++ ++ P R ++ C + C H F CD +S + + +++D Q + ++ R DN L T+L N ++L +F + + +V ++C+ ++ P+ + YE Y + D + L + P K++L ++ +I V ++ ++ I + + ++ Sbjct: 16 NAVYQNGTPFLKFHKVESATLRNGSFSYFGANKPIVIDGMFPQVTIQIVELINNAPPKLEFHCVVDKLSKDSPITSAQLCFETPFSRKLTCDNALGSSYLLSDKF-KFKSMGDVYNGYIFTSNWDRDKFPLDATYFCRLGNSNGVIQSNSIEVQDIDSYTTAFIKRQSLDP---ILRGPIVGSSFPMSLECGGSMD--HVELPNWAKQTFPVPYDWVACEAEADS-QLEIRKCYDRSQPIAEYFEHTVLSNGTLIIHDLVKDWRPIGILCTTSIVRNKIFSAYFQDNDGKAVPFTYIPGTYPNQIIPRSPQHNRVALRSGWHARDIITLNAFYTANPRTVSAKWAKDDSSLPIQFTSNRHSLVFPQDIKPSYAGTYVLSLKS--PYGQLRFKYEVSVEQPPTIK---EAPPRHLIVP-QGNQAQVVGEFSGRLTSRSLLINGIQIDAVSLVRYRESLRLLQESFPYVGDLNPEIEFPSDTSIRYTVRDLAFAPN--------SPYGSSHIVTMVVTNALGSVRTNTLIRVLPKPDVIRQLSDYSCVEDCFNHTTHRFYCDIEMAPYSGLRVVKTWELDGQDLQRLDPR-----DNRL-TFLKYLNREVVLNIAQDNALFDELFANVDLSCRFRVFGPETDLFAGYMYETPYYDTKNDPALHAMLTAHIRKYPS-KSKLAAVSSISWIVAVVIAVLILLIVAILAVCIVMR 697
BLAST of SMED30005411 vs. TrEMBL
Match: A0A183T8D5 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1) HSP 1 Score: 97.8265 bits (242), Expect = 3.142e-17 Identity = 100/406 (24.63%), Postives = 173/406 (42.61%), Query Frame = 2 Query: 896 IIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFK--WEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSG----VRF--ENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREIS---EEDNLLKTYLNITNGSLILKRIFSDAWND-----VKIACKLTIYAPK----VFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLIS------IEKTIITVSLISVIYICGIALFLK 2035 I+ S+ + +R G +L+A YT + K W K L F T+ L FP+ +T G Y L +T + +VF TF+V + + P I + P +VY+ QP ++ +G ++ IN LI +S A + S+ +L +FPY+ G + F + SIR L+ + G +V+ + G + +T++ ++ LP R + C D C H FLCD Y N + W E +GR++ ++D LL T+L I +IL + +A D +++C+ IYAP+ +N E +Y + D + L + P T + S I +I V ++ + I + + ++ Sbjct: 156 IVPKSLQSVSKDYRAGQYAKGTIELTALYTANPLKTKTAWTKNGGPLPILFETTDHSLIFPEEITPADSGNYSLTVTGPSQREVF--TFNVLVNAPPKIRRM--APRSVYVLEGQPVQIETEFEGRLTSRSVSINGILIDTLSTALFEESLKHLRDQFPYIDGLYPDIEFPSDTSIRYTLRDITSSAN--------APYGASHSVTLTGTNTLGSVHTQTVIRVLSLPRITRLMADSSCEDDCFNGTSHRFLCDVDLAPYAGLN--VVKTW--EFDGRDLEALHQQDPLL-TFLKIARNEVILSIVAGNATFDRLFASAELSCRFRIYAPEEEELFGVN---AMEPLYDTKTDPELHKLLTAAIRKNPSGNTSIASFTSLSWIAAVVIAVMILLAVIILAVCVVMR 541
BLAST of SMED30005411 vs. TrEMBL
Match: A0A3P7D477 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS12733 PE=4 SV=1) HSP 1 Score: 97.8265 bits (242), Expect = 3.254e-17 Identity = 100/406 (24.63%), Postives = 173/406 (42.61%), Query Frame = 2 Query: 896 IIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFK--WEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSG----VRF--ENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREIS---EEDNLLKTYLNITNGSLILKRIFSDAWND-----VKIACKLTIYAPK----VFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLIS------IEKTIITVSLISVIYICGIALFLK 2035 I+ S+ + +R G +L+A YT + K W K L F T+ L FP+ +T G Y L +T + +VF TF+V + + P I + P +VY+ QP ++ +G ++ IN LI +S A + S+ +L +FPY+ G + F + SIR L+ + G +V+ + G + +T++ ++ LP R + C D C H FLCD Y N + W E +GR++ ++D LL T+L I +IL + +A D +++C+ IYAP+ +N E +Y + D + L + P T + S I +I V ++ + I + + ++ Sbjct: 156 IVPKSLQSVSKDYRAGQYAKGTIELTALYTANPLKTKTAWTKNGGPLPILFETTDHSLIFPEEITPADSGNYSLTVTGPSQREVF--TFNVLVNAPPKIRRM--APRSVYVLEGQPVQIETEFEGRLTSRSVSINGILIDTLSTALFEESLKHLRDQFPYIDGLYPDIEFPSDTSIRYTLRDITSSAN--------APYGASHSVTLTGTNTLGSVHTQTVIRVLSLPRITRLMADSSCEDDCFNGTSHRFLCDVDLAPYAGLN--VVKTW--EFDGRDLEALHQQDPLL-TFLKIARNEVILSIVAGNATFDRLFASAELSCRFRIYAPEEEELFGVN---AMEPLYDTKTDPELHKLLTAAIRKNPSGNTSIASFTSLSWIAAVVIAVMILLAVIILAVCVVMR 541
BLAST of SMED30005411 vs. Planmine SMEST
Match: SMESG000070139.1 (SMESG000070139.1) HSP 1 Score: 1466.44 bits (3795), Expect = 0.000e+0 Identity = 718/718 (100.00%), Postives = 718/718 (100.00%), Query Frame = 2 Query: 2 IYFKVIFIIFLPNFASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLIGKQPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVSGNSNFLRGFYFCRLENQFDSIVSNNIEIHNIHYYRSLIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFKWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISEEDNLLKTYLNITNGSLILKRIFSDAWNDVKIACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEKTIITVSLISVIYICGIALFLKFIWLLPLEYWHISAPRNESVNINNLHQDPTSTPLLPIAIS 2155 IYFKVIFIIFLPNFASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLIGKQPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVSGNSNFLRGFYFCRLENQFDSIVSNNIEIHNIHYYRSLIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFKWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISEEDNLLKTYLNITNGSLILKRIFSDAWNDVKIACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEKTIITVSLISVIYICGIALFLKFIWLLPLEYWHISAPRNESVNINNLHQDPTSTPLLPIAIS Sbjct: 3 IYFKVIFIIFLPNFASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLIGKQPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFEKFAIEFDNQTGFIVSGNSNFLRGFYFCRLENQFDSIVSNNIEIHNIHYYRSLIKESFKPNTDLNSQPIKTVADSHLLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFKWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISEEDNLLKTYLNITNGSLILKRIFSDAWNDVKIACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEKTIITVSLISVIYICGIALFLKFIWLLPLEYWHISAPRNESVNINNLHQDPTSTPLLPIAIS 720
BLAST of SMED30005411 vs. Planmine SMEST
Match: SMESG000064374.1 (SMESG000064374.1) HSP 1 Score: 192.971 bits (489), Expect = 6.970e-51 Identity = 177/693 (25.54%), Postives = 309/693 (44.59%), Query Frame = 2 Query: 41 FASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLIGK------QPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLG-FEKFAIEFDNQTGFIVSGNSNF---------LRGFYFCRLENQFDSIVSNNIEIHNIHYYRS-------LIKESFKPNTDLNSQPIKT-VADSH--LLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECF--------KNEILLQPNVDYVQFNNFSIHLFD---HVYTKYARF------VLESFSVIEKGPWNKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFK----WEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISEEDNL-----LKTYLNITNGSLILKRIFSDAWNDVK----------IACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTI 1933 ++ LE+ L+F P+ V +RK+ Y + + +S+DA+ S Q+L+ I F+F+C LE F + A C K + E C I++ Q I +F +FD TG I N + GFY+C+LEN SI SN IEI + +Y++ I F P+ PI +DS LD G + + ++IPKW + + S W++CP D T S +CF K+ L+ +DY QF N + L + Y K F V + +S N S ++ + I + S +N + REG +KL A Y G+ ++ K W+K SL + F Q +L FP ++ G Y LK+ + F ++V +VS P + + ++ + Y+ ++ + + + K +L++N+ I L++ + + S+ NL +FP+L+G+ +I + E K N +K+ + + V+F E+ GV ++ ++ + E P + +N +C D C D+ H F C +Y K F I W +NG + DN+ +K++L + N ++ +K I S K ++C++ I + E YS+D++ ++ L+ +V + Sbjct: 13 YSISGLEKFLQFIPHKDVTIRKSVYELDEDPVSVDAFQSSQVLIKYIQSIRDLNFPEFKFYCHVKELETNFKISNAYLCKKQSIMGHEDCHLISKDTQKIKFQEHMQFETKFDPGTGIIADLNMDISAVKSRLQPFDGFYYCKLENHMGSISSNYIEIQDFEFYKTKLSNFINQISVRFAPS------PINDFTSDSFPIYLDCGKLVGNYEENIPKWANKDLLSSFTWQLCP----DAT-SGIQCFSSVVDTQKKSSRPLEEFIDYYQFPNGTAVLLGIKGNDYIKAGLFCGLKAIVPKMYSAYLVKTKNALWRPISYTVVGKYNKKIKQLSAENTEITIREGKYRSSRFKLYALYVGNPDHTKIKAEWKKDTYSLPSTFKQGPYQLVFPIEISESYSGKYDLKIIQSEPFDTVTFRYNVKVVSSPKLKDNIKQQSVFYVTVDKSAQISVFFERDITKLSLEVNNMPIDLLTAKAKEKSVKNLCYKFPFLTGIDISANISLNEGMIYYEAK-NLIKRSNSPQAKTHTVTFIAENDIGVSKVSYLIRVFENPTFIYYLNDFNCTDNCMDDQRHDFKCSINETEYKKEGFTIKYVWT--INGLSV---DNMQSGYAIKSFLEVNNDTITIKPIKSANLEKYKEMLGYFSKSNLSCRVDI------VPTSNPSETFYSTDQNPNLRPYLQAMVNL 682
BLAST of SMED30005411 vs. Planmine SMEST
Match: SMESG000064374.1 (SMESG000064374.1) HSP 1 Score: 192.971 bits (489), Expect = 7.638e-51 Identity = 177/693 (25.54%), Postives = 309/693 (44.59%), Query Frame = 2 Query: 41 FASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLIGK------QPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLG-FEKFAIEFDNQTGFIVSGNSNF---------LRGFYFCRLENQFDSIVSNNIEIHNIHYYRS-------LIKESFKPNTDLNSQPIKT-VADSH--LLDFGIEINDTYKSIPKWIFQKIEKSIKWRICPVNYDDFTYSWAECF--------KNEILLQPNVDYVQFNNFSIHLFD---HVYTKYARF------VLESFSVIEKGPWNKFRIIGSVEMLNSLSGDIIKYSIDNEHLYFREGFSNFFEYKLSAAYTGDVENFK----WEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVYIFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISEEDNL-----LKTYLNITNGSLILKRIFSDAWNDVK----------IACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTI 1933 ++ LE+ L+F P+ V +RK+ Y + + +S+DA+ S Q+L+ I F+F+C LE F + A C K + E C I++ Q I +F +FD TG I N + GFY+C+LEN SI SN IEI + +Y++ I F P+ PI +DS LD G + + ++IPKW + + S W++CP D T S +CF K+ L+ +DY QF N + L + Y K F V + +S N S ++ + I + S +N + REG +KL A Y G+ ++ K W+K SL + F Q +L FP ++ G Y LK+ + F ++V +VS P + + ++ + Y+ ++ + + + K +L++N+ I L++ + + S+ NL +FP+L+G+ +I + E K N +K+ + + V+F E+ GV ++ ++ + E P + +N +C D C D+ H F C +Y K F I W +NG + DN+ +K++L + N ++ +K I S K ++C++ I + E YS+D++ ++ L+ +V + Sbjct: 14 YSISGLEKFLQFIPHKDVTIRKSVYELDEDPVSVDAFQSSQVLIKYIQSIRDLNFPEFKFYCHVKELETNFKISNAYLCKKQSIMGHEDCHLISKDTQKIKFQEHMQFETKFDPGTGIIADLNMDISAVKSRLQPFDGFYYCKLENHMGSISSNYIEIQDFEFYKTKLSNFINQISVRFAPS------PINDFTSDSFPIYLDCGKLVGNYEENIPKWANKDLLSSFTWQLCP----DAT-SGIQCFSSVVDTQKKSSRPLEEFIDYYQFPNGTAVLLGIKGNDYIKAGLFCGLKAIVPKMYSAYLVKTKNALWRPISYTVVGKYNKKIKQLSAENTEITIREGKYRSSRFKLYALYVGNPDHTKIKAEWKKDTYSLPSTFKQGPYQLVFPIEISESYSGKYDLKIIQSEPFDTVTFRYNVKVVSSPKLKDNIKQQSVFYVTVDKSAQISVFFERDITKLSLEVNNMPIDLLTAKAKEKSVKNLCYKFPFLTGIDISANISLNEGMIYYEAK-NLIKRSNSPQAKTHTVTFIAENDIGVSKVSYLIRVFENPTFIYYLNDFNCTDNCMDDQRHDFKCSINETEYKKEGFTIKYVWT--INGLSV---DNMQSGYAIKSFLEVNNDTITIKPIKSANLEKYKEMLGYFSKSNLSCRVDI------VPTSNPSETFYSTDQNPNLRPYLQAMVNL 683
BLAST of SMED30005411 vs. Planmine SMEST
Match: SMESG000064379.1 (SMESG000064379.1) HSP 1 Score: 153.295 bits (386), Expect = 5.511e-38 Identity = 183/757 (24.17%), Postives = 317/757 (41.88%), Query Frame = 2 Query: 14 VIFIIFLPNFASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLI-----GKQPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFE-KFAIEFDNQTGFIVS----GNSNFLR-----GFYFCRLENQFDSIVSNNIEIHNIHYYRS----LIKESFKPNTDLN--------SQPIKTVADSHLLDFGIEINDTYKSIPKWI-FQKIEKSIKWRIC-PVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKFRIIGSVE---------MLNSLSGDIIKYSIDNEHLYFREG-FSNFFEYKLSAAYTGDVE----NFKWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVY----IFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISE-EDNLLKTYLNITNGSLILK--------------RIFSDAWNDVKIACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEKTIITVSLISVIYICGIALFLKFIWLLPLEYWHISAPRNESVN 2098 ++ F+P+ LE+ L+F P+ V+ R+T Y ++ DAYL Q+L+ + F+C PL+ + + KA FC K L+ E C I + IS GF F+I FD+ G I+ G F R GFY+C+L N SI SN EI + +YR L F + D+ S P + + +L + K++PKW+ + + ++WR C P + + E N L+ QF N SI L T Y L P E LNS I + + ++ REG + N +KL A Y + + ++W K + L + F T EL FP + G+Y L ++ F + V I P + + P+ +Y FP S + + + +L+IN+ I ++S+ + + + LF ++ +L+G+ +I +E EVK N K L + G+ V+F E+P GV ++ ++ I+E P + + C + C H+F C +Y K F T+++ +NG ++ + + YL+I ++ LK R+FS++ ++CK+ I F N + + Y+SD +K LE + + + +S+ +I + L ++ + + L L F + +++ + S N Sbjct: 4 LMLFYFIPSIFG--LEKFLQFTPHKDVRERRTSYQFNELSINYDAYLDSQVLIKYLLHYTYASNNVTFYCHVKPLDKNYKITKAYFCKKGSLTTQEDCYEIYKKTHEISFGFHFNFSIIFDSTEGMIIRLSIRGYHTFRRARPFHGFYYCKLYNILGSISSNYAEIQDHEFYRLRWTLLFDYRFNFDNDVRRTAHFTSESFPFYILCCNAILKYE-------KNVPKWLNYDLFSQQLEWRFCRPFHRNRRCLMNNEA--NYTFLEDFFHCYQFPNGSIVLMGLKRTDYRESGLLCSIDTSDDPQYLSEYFFKNETRWQYIYYYALNSYRRKIKFLTETDFNITTREGIYDNKTRFKLYALYVSNPDPKSIAYEWRKDNARLPSTFTGTSHELLFPIEIYDSDSGLYELTVSQSKPPDNATFRYKVTISCPPKLKDRSTRPSVIYSLDDFFPRLSLSFERDITNL----SLEINNIPIDVLSETNKENDLKRLFLKYEFLTGIDISANISVSQGLIEYEVK-NLTKNLNTSEGKTHIVTFIAENPTGVSKISYLIRILENPKFIHYLKDFSCINNCSNQDKHIFKCAIEELEYKKIGFL--TKYVWSLNGLSLNYVKGHEQMIYLDINRDTITLKPFKSENPIRDQRMTRLFSNSI----LSCKVII----SFNNKDIR----YNSDNHPNVKSLLEAKIKLQENQIN--VSLTSWMIPLILSFIVMVAVVTLTLYFCQKFSGKTYNVDQNGSTSAN 728
BLAST of SMED30005411 vs. Planmine SMEST
Match: SMESG000064379.1 (SMESG000064379.1) HSP 1 Score: 153.295 bits (386), Expect = 5.562e-38 Identity = 181/743 (24.36%), Postives = 311/743 (41.86%), Query Frame = 2 Query: 56 LERILRFAPNPAVKLRKTEYTVRNEKLSIDAYLSQQILVTLI-----GKQPFQFFCQTYPLEIQFIVIKAEFCWKLFLSDSEKCSTINEPKQSISLGFE-KFAIEFDNQTGFIVS----GNSNFLR-----GFYFCRLENQFDSIVSNNIEIHNIHYYRS----LIKESFKPNTDLN--------SQPIKTVADSHLLDFGIEINDTYKSIPKWI-FQKIEKSIKWRIC-PVNYDDFTYSWAECFKNEILLQPNVDYVQFNNFSIHLFDHVYTKYARFVLESFSVIEKGPWNKFRIIGSVE---------MLNSLSGDIIKYSIDNEHLYFREG-FSNFFEYKLSAAYTGDVE----NFKWEKGLSSLSAKFVQTERELFFPKLVTRDLEGIYYLKLTSLNNYKVFDFTFHVHIVSLPVISNILQIPNNVY----IFPNQPTSMKLVVKGMNLKGTLKINSHLISLISKASHKTSMVNLFQRFPYLSGVRFENSIRTELKFLEMEVKLNKMKKLLENRGQFLAVSFEVESPQGVLRLRTIVEIVELPVWRRTVNQVDCFDVCHGDRDHLFLCDTFSDQYIKRNFKIDTQWMVEVNGREISE-EDNLLKTYLNITNGSLILK--------------RIFSDAWNDVKIACKLTIYAPKVFINHETQYEKIYSSDEDERIKGSLEKVVTIVPGRKTRLISIEKTIITVSLISVIYICGIALFLKFIWLLPLEYWHISAPRNESVN 2098 LE+ L+F P+ V+ R+T Y ++ DAYL Q+L+ + F+C PL+ + + KA FC K L+ E C I + IS GF F+I FD+ G I+ G F R GFY+C+L N SI SN EI + +YR L F + D+ S P + + +L + K++PKW+ + + ++WR C P + + E N L+ QF N SI L T Y L P E LNS I + + ++ REG + N +KL A Y + + ++W K + L + F T EL FP + G+Y L ++ F + V I P + + P+ +Y FP S + + + +L+IN+ I ++S+ + + + LF ++ +L+G+ +I +E EVK N K L + G+ V+F E+P GV ++ ++ I+E P + + C + C H+F C +Y K F T+++ +NG ++ + + YL+I ++ LK R+FS++ ++CK+ I F N + + Y+SD +K LE + + + +S+ +I + L ++ + + L L F + +++ + S N Sbjct: 12 LEKFLQFTPHKDVRERRTSYQFNELSINYDAYLDSQVLIKYLLHYTYASNNVTFYCHVKPLDKNYKITKAYFCKKGSLTTQEDCYEIYKKTHEISFGFHFNFSIIFDSTEGMIIRLSIRGYHTFRRARPFHGFYYCKLYNILGSISSNYAEIQDHEFYRLRWTLLFDYRFNFDNDVRRTAHFTSESFPFYILCCNAILKYE-------KNVPKWLNYDLFSQQLEWRFCRPFHRNRRCLMNNEA--NYTFLEDFFHCYQFPNGSIVLMGLKRTDYRESGLLCSIDTSDDPQYLSEYFFKNETRWQYIYYYALNSYRRKIKFLTETDFNITTREGIYDNKTRFKLYALYVSNPDPKSIAYEWRKDNARLPSTFTGTSHELLFPIEIYDSDSGLYELTVSQSKPPDNATFRYKVTISCPPKLKDRSTRPSVIYSLDDFFPRLSLSFERDITNL----SLEINNIPIDVLSETNKENDLKRLFLKYEFLTGIDISANISVSQGLIEYEVK-NLTKNLNTSEGKTHIVTFIAENPTGVSKISYLIRILENPKFIHYLKDFSCINNCSNQDKHIFKCAIEELEYKKIGFL--TKYVWSLNGLSLNYVKGHEQMIYLDINRDTITLKPFKSENPIRDQRMTRLFSNSI----LSCKVII----SFNNKDIR----YNSDNHPNVKSLLEAKIKLQENQIN--VSLTSWMIPLILSFIVMVAVVTLTLYFCQKFSGKTYNVDQNGSTSAN 724 The following BLAST results are available for this feature:
BLAST of SMED30005411 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005411 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005411 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30005411 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005411 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005411 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005411 ID=SMED30005411|Name=SMED30005411|organism=Schmidtea mediterranea sexual|type=transcript|length=2659bpback to top protein sequence of SMED30005411-orf-1 >SMED30005411-orf-1 ID=SMED30005411-orf-1|Name=SMED30005411-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=719bp IYFKVIFIIFLPNFASENLERILRFAPNPAVKLRKTEYTVRNEKLSIDAYback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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