SMED30005359

Overview
NameSMED30005359
Smed IDSMED30005359
Length (bp)899
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005359 (SMED30005359) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005359 (SMED30005359) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005359 (SMED30005359) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005359 (SMED30005359) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005359

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30005359SMESG000033501.1 dd_Smed_v4_16282_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30005359SMESG000033501.1 dd_Smed_v4_16282_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30005359 vs. TrEMBL
Match: A0A267EBH1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022930g2 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.486e-9
Identity = 44/113 (38.94%), Postives = 63/113 (55.75%), Query Frame = 2
Query:  242 RLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQ--KIKSKSMKDLLSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGG---DIPNKYLRKSFSSRL 565
            R+ GDLP+NVTE RL    L +L+ +R ++  E    +N FID Q  K +SKS+  LL  +DI QYR++  + VE   Q+S   K  I+ +R  G   D  +K+      SRL
Sbjct:   58 RMGGDLPENVTEERLYQTRLYDLALERKKMMNESDYVRNQFIDHQVRKARSKSLGRLLQGMDIEQYRNKSASGVEKLHQRSVHAKLMILANRRLGLSIDEVSKFTEAELRSRL 170          
BLAST of SMED30005359 vs. TrEMBL
Match: A0A267FKE4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022930g1 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.509e-9
Identity = 44/113 (38.94%), Postives = 63/113 (55.75%), Query Frame = 2
Query:  242 RLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQ--KIKSKSMKDLLSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGG---DIPNKYLRKSFSSRL 565
            R+ GDLP+NVTE RL    L +L+ +R ++  E    +N FID Q  K +SKS+  LL  +DI QYR++  + VE   Q+S   K  I+ +R  G   D  +K+      SRL
Sbjct:   58 RMGGDLPENVTEERLYQTRLYDLALERKKMMNESDYVRNQFIDHQVRKARSKSLGRLLQGMDIEQYRNKSASGVEKLHQRSVHAKLMILANRRLGLSIDEVSKFTEAELRSRL 170          
BLAST of SMED30005359 vs. TrEMBL
Match: A0A267FTB8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022930g3 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.532e-9
Identity = 44/113 (38.94%), Postives = 63/113 (55.75%), Query Frame = 2
Query:  242 RLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQ--KIKSKSMKDLLSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGG---DIPNKYLRKSFSSRL 565
            R+ GDLP+NVTE RL    L +L+ +R ++  E    +N FID Q  K +SKS+  LL  +DI QYR++  + VE   Q+S   K  I+ +R  G   D  +K+      SRL
Sbjct:   58 RMGGDLPENVTEERLYQTRLYDLALERKKMMNESDYVRNQFIDHQVRKARSKSLGRLLQGMDIEQYRNKSASGVEKLHQRSVHAKLMILANRRLGLSIDEVSKFTEAELRSRL 170          
BLAST of SMED30005359 vs. Planmine SMEST
Match: SMESG000033501.1 (SMESG000033501.1)

HSP 1 Score: 488.034 bits (1255), Expect = 8.893e-177
Identity = 238/242 (98.35%), Postives = 239/242 (98.76%), Query Frame = 2
Query:  125 MNGSNTIQKTVESPSKSQREIVKWAYSSLYTPENKIAYERLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQKIKSKSMKDLLSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGGDIPNKYLRKSFSSRLMTNSDMRVSSAGLPILPKSDNKVIHSARTCRTLDDRYWSQVTSDRRHKELIDLLSPVSLQFQKALDVCEIVKSIDSLTPLIDQKLYMEDTKSGFS 850
            MNGSNTIQKTVESPSKSQREIVKWAYSSLYTPENKIAYERLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQKIKSKSMKD LSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGGDIPNKYLRKSFSSRLMTNSDMRVSSAGLPILP+SDNKVIHSARTCRTLDDRYWSQV SDRRHKELIDLLSPVSLQFQKALDVCEIVKSIDSLTP IDQKLYMEDTKSGFS
Sbjct:    1 MNGSNTIQKTVESPSKSQREIVKWAYSSLYTPENKIAYERLTGDLPKNVTEIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQKIKSKSMKDFLSKIDINQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGGDIPNKYLRKSFSSRLMTNSDMRVSSAGLPILPQSDNKVIHSARTCRTLDDRYWSQVASDRRHKELIDLLSPVSLQFQKALDVCEIVKSIDSLTPFIDQKLYMEDTKSGFS 242          
The following BLAST results are available for this feature:
BLAST of SMED30005359 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A267EBH12.486e-938.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FKE42.509e-938.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FTB82.532e-938.94Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30005359 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005359 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000033501.18.893e-17798.35SMESG000033501.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005359 ID=SMED30005359|Name=SMED30005359|organism=Schmidtea mediterranea sexual|type=transcript|length=899bp
TCTGTCTTCATCAACTGTGGTGTAGTTTAACAGAGCTGAGTGCAAAGTGT
ACATTAAATTGTCACGCTTGACTGATTTCGTTATTTGTAGCATATGCAAT
AATCATTATTAAGAATTTACATTTATGAACGGCTCAAACACAATCCAAAA
AACAGTCGAATCTCCTTCTAAATCTCAACGTGAAATTGTCAAGTGGGCAT
ATAGTTCTTTGTATACACCAGAGAATAAAATCGCTTATGAAAGACTAACA
GGAGATTTGCCGAAGAACGTCACCGAAATCCGATTACTCCACAAGAACTT
AATGAATCTGTCAAGACAACGGTCAAGAGTGCAACAAGAATTTATATTGA
GAAAAAATAATTTCATTGATTCTCAGAAAATCAAGTCAAAATCCATGAAA
GATTTATTGTCTAAAATAGATATCAATCAGTATCGCGATAGGGATACCAA
TGCTGTGGAGATTTATAAACAGAAATCGTTTGAAATCAAAGAGAAAATTA
TTCGAGATCGTTTTGGCGGTGACATACCGAATAAATATTTACGGAAATCG
TTTTCTAGTAGACTAATGACAAATTCGGATATGCGCGTTTCATCCGCTGG
TTTGCCAATTCTTCCGAAATCGGATAATAAAGTGATCCATTCTGCCAGAA
CTTGTAGAACTTTGGATGATAGATACTGGAGTCAGGTTACCAGTGACAGA
CGACACAAGGAATTGATAGATTTGTTATCTCCGGTATCTCTGCAATTTCA
AAAGGCCCTCGATGTCTGTGAAATTGTCAAGTCGATAGATTCACTCACCC
CCCTTATTGATCAGAAACTTTATATGGAAGACACTAAAAGTGGATTTTCT
TGAAATTTTAAAGCATTAATCGAAATTAAAGCATTTGTTTCTTTACCCC
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protein sequence of SMED30005359-orf-1

>SMED30005359-orf-1 ID=SMED30005359-orf-1|Name=SMED30005359-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MNGSNTIQKTVESPSKSQREIVKWAYSSLYTPENKIAYERLTGDLPKNVT
EIRLLHKNLMNLSRQRSRVQQEFILRKNNFIDSQKIKSKSMKDLLSKIDI
NQYRDRDTNAVEIYKQKSFEIKEKIIRDRFGGDIPNKYLRKSFSSRLMTN
SDMRVSSAGLPILPKSDNKVIHSARTCRTLDDRYWSQVTSDRRHKELIDL
LSPVSLQFQKALDVCEIVKSIDSLTPLIDQKLYMEDTKSGFS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 52..72