SMED30005322

Overview
NameSMED30005322
Smed IDSMED30005322
Length (bp)603
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005322 (SMED30005322) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005322 (SMED30005322) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005322 (SMED30005322) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005322 (SMED30005322) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005322

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30005322SMESG000078208.1 Contig1486GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005322SMESG000078208.1 Contig44272newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005322SMESG000078208.1 Contig44272uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30005322SMESG000078208.1 dd_2727dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
enteric muscle cellSMED30005322SMESG000078208.1 dd_Smed_v4_2727_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005322 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004742:18469..19066 +2906
Homology
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BLAST of SMED30005322 vs. TrEMBL
Match: A0A267F9X9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026293g1 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.345e-10
Identity = 51/138 (36.96%), Postives = 72/138 (52.17%), Query Frame = 2 
Query:   68 ITFHQVTMSLLISTVAFSITSVVTKNWD-DSRSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTXXXXXXXXXXXXXXXXXXXXYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 478
            ITFHQ+T+  L   +A SI S  T+ W   S  +++L N +     K F IIG      + LME+L+  S+TF LS A+ +L  I  LL   SLL+ + +   +   G  G+S W     +V   +A  FY I WRCT
Sbjct:    6 ITFHQITIITLCFGIACSIASCATRAWQSGSGGLFDLPNEAWANTIKAFAIIGVAMLGFSLLMEILMVASNTFKLSRAMHLLCFICCLLSAASLLISLGLYSRHYSYG--GYSVWLLTAGTVFALEAFFFYTIQWRCT 141
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BLAST of SMED30005322 vs. TrEMBL
Match: A0A267EGW5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016342g1 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 7.837e-10
Identity = 50/137 (36.50%), Postives = 71/137 (51.82%), Query Frame = 2 
Query:   68 ITFHQVTMSLLISTVAFSITSVVTKNWDDSRSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTXXXXXXXXXXXXXXXXXXXXYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 478
            ITFHQ+T+  L   +A SI S  T+ W  S  +++L N +     K F IIG      +  ME+L+  S+TF LS A+ +L  I  LL   SLL+ + +   +   G  G+S W     +V   +A  FY I WRCT
Sbjct:    6 ITFHQITIITLCFGIACSIASCATRAWK-SGGLFDLPNEAWANTIKAFAIIGVAMLGFSLFMEILMVASNTFKLSRAMHLLCFICCLLSAASLLISLGLYSRHYSYG--GYSVWLLTAGTVFALEAFFFYTIQWRCT 139
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BLAST of SMED30005322 vs. TrEMBL
Match: A0A1I8IAF0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 4.470e-8
Identity = 45/146 (30.82%), Postives = 76/146 (52.05%), Query Frame = 2 
Query:   56 TMVVITFHQVTMSLLISTVAFSITSVVTKNWDDS-----RSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTXXXXXXXXXXXXXXXXXXXXYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 478
            +M  I+FHQ+ +++L   +A  I +  + +W  S       +++L N+S     K   IIG        L+E+L+ +S+TF LS A+ +L L+  ++    LL+G+ V       G  G+S W    S+V   +A+ FY+I WRC 
Sbjct:    6 SMATISFHQICITVLSLGLACGIIACASSSWQMSWNARGSGLFDLPNNSEGNSVKALTIIGVAFLAFGLLLEILMIVSNTFKLSKAVNLLCLVCCIIAVAGLLIGLIVYAAKFSYG--GYSVWLLTASTVFAIEALFFYIIQWRCA 149
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BLAST of SMED30005322 vs. TrEMBL
Match: A0A1I8IRB7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig011666g1 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 6.515e-8
Identity = 45/145 (31.03%), Postives = 75/145 (51.72%), Query Frame = 2 
Query:   59 MVVITFHQVTMSLLISTVAFSITSVVTKNWDDS-----RSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTXXXXXXXXXXXXXXXXXXXXYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 478
            M  I+FHQ+ +++L   +A  I +  + +W  S       +++L N+S     K   IIG        L+E+L+ +S+TF LS A+ +L L+  ++    LL+G+ V       G  G+S W    S+V   +A+ FY+I WRC 
Sbjct:    1 MATISFHQICITVLSLGLACGIIACASSSWQMSWNARGSGLFDLPNNSEGNSVKALTIIGVAFLAFGLLLEILMIVSNTFKLSKAVNLLCLVCCIIAVAGLLIGLIVYAAKFSYG--GYSVWLLTASTVFAIEALFFYIIQWRCA 143
[Close]
BLAST of SMED30005322 vs. Planmine SMEST
Match: SMESG000078208.1 (SMESG000078208.1)

HSP 1 Score: 234.187 bits (596), Expect = 1.203e-79
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 2 
Query:   59 MVVITFHQVTMSLLISTVAFSITSVVTKNWDDSRSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTXXXXXXXXXXXXXXXXXXXXYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 478
            MVVITFHQVTMSLLISTVAFSITSVVTKNWDDSRSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTLIFVLLGFGSLLVGVSVGGVYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT
Sbjct:    1 MVVITFHQVTMSLLISTVAFSITSVVTKNWDDSRSIWELTNHSNDVVTKVFVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTLIFVLLGFGSLLVGVSVGGVYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT 140
The following BLAST results are available for this feature:
BLAST of SMED30005322 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A267F9X91.345e-1036.96Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EGW57.837e-1036.50Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IAF04.470e-830.82Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IRB76.515e-831.03Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30005322 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005322 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000078208.11.203e-79100.00SMESG000078208.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005322 ID=SMED30005322|Name=SMED30005322|organism=Schmidtea mediterranea sexual|type=transcript|length=603bp
TCTATATAAATCTTATATGTTTTTAATTAGAAAATATATTATTTTTGTTT
TTTGAACAATGGTTGTCATTACATTCCATCAAGTGACAATGTCATTGCTG
ATATCTACAGTTGCTTTTTCCATAACGTCAGTGGTGACGAAAAATTGGGA
CGATTCACGCAGCATCTGGGAGCTAACCAACCATTCTAATGACGTCGTTA
CGAAGGTGTTTGTCATTATCGGCACAGTTTGTGCTTGTCTGGCGTTTCTG
ATGGAACTCCTCATTCAACTCTCGTCAACATTTTCTCTCAGTAATGCAAT
ACGAATATTGACTTTGATTTTCGTTTTACTCGGATTTGGATCTTTATTAG
TCGGAGTGAGCGTCGGTGGGGTTTATCGGTGCGCAGGAGAATGTGGATTT
TCCTATTGGTTTGCTGCTATTAGCAGTGTTCTAATGTTTCAAGCTGTCAT
TTTCTACATGATAGCTTGGAGATGTACATAACTTGATATTAGAAAGCAGT
GGCATGACTAACCTTATATTCAACAAGATTATGAATATTATTATACTGAG
TCAGATCTTTGAATTTTAGTTTCAAAAATATATTTCCTTAATTTAAAAAA
AAA
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protein sequence of SMED30005322-orf-1

>SMED30005322-orf-1 ID=SMED30005322-orf-1|Name=SMED30005322-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=141bp
MVVITFHQVTMSLLISTVAFSITSVVTKNWDDSRSIWELTNHSNDVVTKV
FVIIGTVCACLAFLMELLIQLSSTFSLSNAIRILTLIFVLLGFGSLLVGV
SVGGVYRCAGECGFSYWFAAISSVLMFQAVIFYMIAWRCT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003104enteric muscle cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 84..106
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 4..26
NoneNo IPR availableTMHMMTMhelixcoord: 113..135