Malate dehydrogenase

Overview
NameMalate dehydrogenase
Smed IDSMED30005321
Length (bp)1805
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Malate dehydrogenase (SMED30005321) t-SNE clustered cells

Violin plots show distribution of expression levels for Malate dehydrogenase (SMED30005321) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Malate dehydrogenase (SMED30005321) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Malate dehydrogenase (SMED30005321) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005321

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30005321SMESG000008924.1 dd_Smed_v4_656_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005321SMESG000008924.1 dd_Smed_v4_656_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30005321SMESG000008924.1 dd_Smed_v6_656_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30005321SMESG000008924.1 dd_Smed_v4_656_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH2 (malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGNC:6971])

HSP 1 Score: 388.267 bits (996), Expect = 7.076e-131
Identity = 187/294 (63.61%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMIC+I NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  GIEKNLG+  +++FE+++I  A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 338

HSP 2 Score: 52.373 bits (124), Expect = 1.199e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH2 (malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGNC:6971])

HSP 1 Score: 308.916 bits (790), Expect = 1.501e-100
Identity = 159/294 (54.08%), Postives = 193/294 (65.65%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMIC+I NP                                           LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  GIEKNLG+  +++FE+++I  A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPG------------------------------------------LDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 296

HSP 2 Score: 52.373 bits (124), Expect = 1.105e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH2 (malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGNC:6971])

HSP 1 Score: 295.434 bits (755), Expect = 2.652e-96
Identity = 141/231 (61.04%), Postives = 179/231 (77.49%), Query Frame = 1 
Query:  358 MTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            MTRDDLFNTNA+IVA L   CAQ CP+AMIC+I NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  GIEKNLG+  +++FE+++I  A+PEL A+IK G +F    K
Sbjct:    1 MTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 231
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: LDHAL6A (lactate dehydrogenase A like 6A [Source:HGNC Symbol;Acc:HGNC:28335])

HSP 1 Score: 62.7734 bits (151), Expect = 5.042e-10
Identity = 76/271 (28.04%), Postives = 117/271 (43.17%), Query Frame = 1 
Query:  187 DRLALYDV--AHVKGVAADLSH----IETPAVVTG--YLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVH-VIGGHSGVTIIPVISQA-------------IPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGI 933
            D L L DV    +KG   DL H    ++ P +V+   YL          NS++V+I AG  +K G TR DL   N SI   ++    Q  P   + I+TNPV+    +A    K +G    R +     LD  R   FI +   L +   S H +I G  G + +PV S               I +   PE   N +  ++ ++G E+V+ K G  S  +S++ A     + IL  ++    +   +     + E  F + P +LG NGI
Sbjct:   46 DELVLVDVDEGKLKGETMDLQHGSPFMKMPNIVSSKDYL-------VTANSNLVIITAGARQKKGETRLDLVQRNVSIFKLMIPNITQYSPHCKLLIVTNPVDILTYVA---WKLSGFPKNRVIGSGCNLDSARFRYFIGQR--LGIHSESCHGLILGEHGDSSVPVWSGVNIAGVPLKDLNPDIGTDKDPEQWEN-VHKKVISSGYEMVKMK-GYTSWGISLSVA--DLTESILKNLRRVHPVSTLSKGLYGINEDIFLSVPCILGENGI 300
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: LDHAL6A (lactate dehydrogenase A like 6A [Source:HGNC Symbol;Acc:HGNC:28335])

HSP 1 Score: 62.7734 bits (151), Expect = 5.042e-10
Identity = 76/271 (28.04%), Postives = 117/271 (43.17%), Query Frame = 1 
Query:  187 DRLALYDV--AHVKGVAADLSH----IETPAVVTG--YLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVH-VIGGHSGVTIIPVISQA-------------IPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGI 933
            D L L DV    +KG   DL H    ++ P +V+   YL          NS++V+I AG  +K G TR DL   N SI   ++    Q  P   + I+TNPV+    +A    K +G    R +     LD  R   FI +   L +   S H +I G  G + +PV S               I +   PE   N +  ++ ++G E+V+ K G  S  +S++ A     + IL  ++    +   +     + E  F + P +LG NGI
Sbjct:   46 DELVLVDVDEGKLKGETMDLQHGSPFMKMPNIVSSKDYL-------VTANSNLVIITAGARQKKGETRLDLVQRNVSIFKLMIPNITQYSPHCKLLIVTNPVDILTYVA---WKLSGFPKNRVIGSGCNLDSARFRYFIGQR--LGIHSESCHGLILGEHGDSSVPVWSGVNIAGVPLKDLNPDIGTDKDPEQWEN-VHKKVISSGYEMVKMK-GYTSWGISLSVA--DLTESILKNLRRVHPVSTLSKGLYGINEDIFLSVPCILGENGI 300
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Celegans
Match: mdh-2 (Probable malate dehydrogenase, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:O02640])

HSP 1 Score: 347.821 bits (891), Expect = 1.880e-115
Identity = 174/290 (60.00%), Postives = 221/290 (76.21%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESN-VTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEF 1035
            K+   V  LALYDV +  GVAADLSHI++ A VT + GP EL   ++N+DV+VIPAGVPRKPGMTRDDLFNTNA IV DL    A+A PKA+I IITNPVNSTVPIA+E+LKKAG YD +R+FGVTTLD+VRS  F++E K  D +K  V V+GGH+G+TIIP++SQ  PS  F E EI+KLT RIQ+AGTEVV AKAGAGSATLSMA AG RF   ++  +KG KN ++CA+V S+ V   ++F+ P+ LGPNG+EK LG+  ++A+E++LI  ++PEL  NI  G+ F
Sbjct:   49 KQDPLVAHLALYDVVNTPGVAADLSHIDSNAKVTAHTGPKELYAAVENADVIVIPAGVPRKPGMTRDDLFNTNAGIVRDLAAVIAKASPKALIAIITNPVNSTVPIASEVLKKAGVYDPKRVFGVTTLDVVRSQAFVSELKGHDASKTVVPVVGGHAGITIIPLLSQVKPSTKFSEEEISKLTPRIQDAGTEVVNAKAGAGSATLSMALAGARFANALVRGIKGEKN-VQCAYVASDAVKGVEYFSTPVELGPNGVEKILGVGKVSAYEQKLIDASVPELNKNIAKGVAF 337
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Fly
Match: Mdh2 (gene:FBgn0262559 transcript:FBtr0083563)

HSP 1 Score: 368.622 bits (945), Expect = 1.363e-123
Identity = 177/291 (60.82%), Postives = 224/291 (76.98%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFAN 1041
            K++  V  LALYD+ H  GVAADLSHI+T +   G++G  +L + LK SDVVVIPAGVPRKPGMTRDDLFN NA I+ D+ +  A+ CPKA++ IITNPVN+ VPIAAEILKKAG YD +RLFGV+TLD+VR+  FI  A  +D   + + VIGGHSGVTI+PV+SQ+ P     +  I KLT+RIQ AGTEVV+AKAGAGSATLSMAYAG RF   +L  + G KN+IEC++V+S VTEA FF+ PL+LG NG+++NLGLP L  +EK+L++ A+PEL  NI+ GI+FAN
Sbjct:   45 KQNPLVTDLALYDIVHTPGVAADLSHIDTKSKTAGFIGADQLGDSLKGSDVVVIPAGVPRKPGMTRDDLFNVNAGIIKDISNSIAKNCPKALVAIITNPVNTCVPIAAEILKKAGVYDPKRLFGVSTLDVVRARAFIGHALGVDPQTVQIPVIGGHSGVTILPVLSQSQPLFKGNQDTIEKLTVRIQEAGTEVVKAKAGAGSATLSMAYAGARFAGSLLKGLNGEKNVIECSYVQSTVTEATFFSTPLVLGKNGVQENLGLPKLNDYEKKLLEAAIPELKKNIQKGIDFAN 335
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Fly
Match: CG10749 (gene:FBgn0036328 transcript:FBtr0075871)

HSP 1 Score: 289.656 bits (740), Expect = 1.173e-92
Identity = 144/292 (49.32%), Postives = 198/292 (67.81%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKN--IIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFA 1038
            K +  +  L+LYD+ +  GV  DLSHI T A V  + G   L + +  +D+VVIPAG+PRKPGM R+DL + NAS+  ++    ++ CP AM+  ITNP+N  VPI A ILK  G YD  RLFGVTTLD+VR+ TF+A+   +D  K+++ VIGGH+G TI+P++SQ  P     + E   L  RIQNAGTEVV AK G GSATLSMA+A  +FV  ++  +KG+K+  I+ECA+VES+VTEA+FFA PL+LGP G+++N GLPDL   E++ +   +P L  +I  GI+  
Sbjct:   48 KHNPQISTLSLYDIKNTTGVGVDLSHINTRASVCPFEGKNGLKKAMDKADIVVIPAGLPRKPGMKREDLVDVNASVACEVAFAASEVCPGAMLAFITNPINVIVPIVATILKAKGTYDPNRLFGVTTLDVVRAQTFVADILNVDPQKVNIPVIGGHTGRTILPILSQCDPPFKGTDKEREALIQRIQNAGTEVVNAKDGLGSATLSMAFAATQFVSSLIKGIKGSKDECIVECAYVESDVTEAQFFATPLILGPQGVKENTGLPDLDDEERKALNGMLPILKESIAKGIKLG 339
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Fly
Match: CG10748 (gene:FBgn0036327 transcript:FBtr0075900)

HSP 1 Score: 281.182 bits (718), Expect = 2.215e-89
Identity = 139/290 (47.93%), Postives = 190/290 (65.52%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFA 1038
            ++   +D LAL+D++ +KG+A DLSHI     V G+ G  EL   +  +DVVV+ AG+PR PGM RD L   N ++   +    + A P+A +  ITNPVN  VP AAE+L   G +D+RRLFG+TTLD+VRS  FI ++  +    +++ VIGGH+G+TI+P+ISQ  P       EI  LT RIQ AGTEVV AKAG GSATLSMAYAG  FV  +L  + G   +IECAFV S +T+A FFA+PL LG +GI++ + LP ++ +EKE ++K +P L  N   G+ FA
Sbjct:   43 RRCPGIDELALHDLSEMKGIATDLSHISQTGKVIGFTGEKELESAVSGADVVVVAAGMPRLPGMQRDHLMAANGNVAVKVATAISNASPRAHLAFITNPVNMIVPAAAEVLMAHGTFDSRRLFGITTLDVVRSKKFIGDSMNISPDDVNIPVIGGHAGITILPLISQCQPIYRCDLQEIQNLTHRIQEAGTEVVNAKAGKGSATLSMAYAGATFVNSLLRGIAGQDGLIECAFVASKLTDAPFFASPLELGKDGIKRYIPLPQMSDYEKEALEKLLPILRQNADEGVNFA 332
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:ZFIN;Acc:ZDB-GENE-040426-2143])

HSP 1 Score: 380.563 bits (976), Expect = 3.923e-128
Identity = 185/294 (62.93%), Postives = 229/294 (77.89%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V  L+L+D+AH  GVAADLSHIET A V GY+G  +L + LK  +VVVIPAGVPRKPGMTRDDLFNTNA+IVA LV GCA+ CP+AMICII+NPVNST+PI +E++KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G+TIIP+ISQ  P V FP  +++ LT RIQ AGTEVV+AKAGAGSATLSMAYAG RF   +L AM G + ++EC+FV S  TE K+F+ PLLLG NGIEKNLGL  L+AFE++L+  AM EL  +IK G +F    K
Sbjct:   44 KNSPLVSELSLFDIAHTPGVAADLSHIETRAHVKGYIGADQLGDALKGCEVVVIPAGVPRKPGMTRDDLFNTNATIVATLVDGCARHCPQAMICIISNPVNSTIPITSEVMKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGITIIPLISQCTPKVEFPADQLSALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFTFSLLDAMNGKEGVVECSFVRSEETECKYFSTPLLLGKNGIEKNLGLGKLSAFEEKLVADAMTELKGSIKKGEDFVANMK 337

HSP 2 Score: 50.0618 bits (118), Expect = 5.017e-6
Identity = 26/36 (72.22%), Postives = 29/36 (80.56%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RS ST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   10 ASLVRSLSTSSQNNAKVAVLGASGGIGQPLSLLLKN 45
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: sssca1 (Sjogren syndrome/scleroderma autoantigen 1 [Source:Xenbase;Acc:XB-GENE-5770639])

HSP 1 Score: 384.03 bits (985), Expect = 1.050e-129
Identity = 189/294 (64.29%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  +  L LYD+AH  GVAADLSHIET A VTGYLG  +L E LK +DVVVIPAGVPRKPGMTRDDLF TNASIVA L   CA+ CP+AMICII+NPVNST+PI +E+ KK G Y+   +FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G TIIP+ISQ+ P V FP+ ++  L  RIQ+AGTEVV+AKAGAGSATLSMAYAG RFV  +L AM G + +IEC+FV S  TE+ +F+ PLLLG NGIEKNLGL  LTAFE++L+ +AM EL A+IK G +F    K
Sbjct:   14 KNSPLISNLTLYDIAHTPGVAADLSHIETRAKVTGYLGAEQLPESLKGADVVVIPAGVPRKPGMTRDDLFTTNASIVATLTEACAKHCPEAMICIISNPVNSTIPITSEVFKKHGVYNPNHIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGKTIIPLISQSTPKVEFPQDQLEALIPRIQDAGTEVVKAKAGAGSATLSMAYAGARFVFSLLDAMNGKEGVIECSFVRSEETESPYFSTPLLLGKNGIEKNLGLGKLTAFEEKLVSEAMGELKASIKKGEDFIKSRK 307
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: sssca1 (Sjogren syndrome/scleroderma autoantigen 1 [Source:Xenbase;Acc:XB-GENE-5770639])

HSP 1 Score: 384.415 bits (986), Expect = 1.213e-129
Identity = 189/294 (64.29%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  +  L LYD+AH  GVAADLSHIET A VTGYLG  +L E LK +DVVVIPAGVPRKPGMTRDDLF TNASIVA L   CA+ CP+AMICII+NPVNST+PI +E+ KK G Y+   +FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G TIIP+ISQ+ P V FP+ ++  L  RIQ+AGTEVV+AKAGAGSATLSMAYAG RFV  +L AM G + +IEC+FV S  TE+ +F+ PLLLG NGIEKNLGL  LTAFE++L+ +AM EL A+IK G +F    K
Sbjct:   25 KNSPLISNLTLYDIAHTPGVAADLSHIETRAKVTGYLGAEQLPESLKGADVVVIPAGVPRKPGMTRDDLFTTNASIVATLTEACAKHCPEAMICIISNPVNSTIPITSEVFKKHGVYNPNHIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGKTIIPLISQSTPKVEFPQDQLEALIPRIQDAGTEVVKAKAGAGSATLSMAYAGARFVFSLLDAMNGKEGVIECSFVRSEETESPYFSTPLLLGKNGIEKNLGLGKLTAFEEKLVSEAMGELKASIKKGEDFIKSRK 318
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: sssca1 (Sjogren syndrome/scleroderma autoantigen 1 [Source:Xenbase;Acc:XB-GENE-5770639])

HSP 1 Score: 384.03 bits (985), Expect = 1.546e-129
Identity = 189/294 (64.29%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  +  L LYD+AH  GVAADLSHIET A VTGYLG  +L E LK +DVVVIPAGVPRKPGMTRDDLF TNASIVA L   CA+ CP+AMICII+NPVNST+PI +E+ KK G Y+   +FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G TIIP+ISQ+ P V FP+ ++  L  RIQ+AGTEVV+AKAGAGSATLSMAYAG RFV  +L AM G + +IEC+FV S  TE+ +F+ PLLLG NGIEKNLGL  LTAFE++L+ +AM EL A+IK G +F    K
Sbjct:   27 KNSPLISNLTLYDIAHTPGVAADLSHIETRAKVTGYLGAEQLPESLKGADVVVIPAGVPRKPGMTRDDLFTTNASIVATLTEACAKHCPEAMICIISNPVNSTIPITSEVFKKHGVYNPNHIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGKTIIPLISQSTPKVEFPQDQLEALIPRIQDAGTEVVKAKAGAGSATLSMAYAGARFVFSLLDAMNGKEGVIECSFVRSEETESPYFSTPLLLGKNGIEKNLGLGKLTAFEEKLVSEAMGELKASIKKGEDFIKSRK 320
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: sssca1 (Sjogren syndrome/scleroderma autoantigen 1 [Source:Xenbase;Acc:XB-GENE-5770639])

HSP 1 Score: 383.645 bits (984), Expect = 2.288e-129
Identity = 189/294 (64.29%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  +  L LYD+AH  GVAADLSHIET A VTGYLG  +L E LK +DVVVIPAGVPRKPGMTRDDLF TNASIVA L   CA+ CP+AMICII+NPVNST+PI +E+ KK G Y+   +FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G TIIP+ISQ+ P V FP+ ++  L  RIQ+AGTEVV+AKAGAGSATLSMAYAG RFV  +L AM G + +IEC+FV S  TE+ +F+ PLLLG NGIEKNLGL  LTAFE++L+ +AM EL A+IK G +F    K
Sbjct:   33 KNSPLISNLTLYDIAHTPGVAADLSHIETRAKVTGYLGAEQLPESLKGADVVVIPAGVPRKPGMTRDDLFTTNASIVATLTEACAKHCPEAMICIISNPVNSTIPITSEVFKKHGVYNPNHIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGKTIIPLISQSTPKVEFPQDQLEALIPRIQDAGTEVVKAKAGAGSATLSMAYAGARFVFSLLDAMNGKEGVIECSFVRSEETESPYFSTPLLLGKNGIEKNLGLGKLTAFEEKLVSEAMGELKASIKKGEDFIKSRK 326
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: sssca1 (Sjogren syndrome/scleroderma autoantigen 1 [Source:Xenbase;Acc:XB-GENE-5770639])

HSP 1 Score: 384.03 bits (985), Expect = 2.302e-129
Identity = 189/294 (64.29%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  +  L LYD+AH  GVAADLSHIET A VTGYLG  +L E LK +DVVVIPAGVPRKPGMTRDDLF TNASIVA L   CA+ CP+AMICII+NPVNST+PI +E+ KK G Y+   +FGVTTLDIVR+NTF+AE K LD  +++V V+GGH+G TIIP+ISQ+ P V FP+ ++  L  RIQ+AGTEVV+AKAGAGSATLSMAYAG RFV  +L AM G + +IEC+FV S  TE+ +F+ PLLLG NGIEKNLGL  LTAFE++L+ +AM EL A+IK G +F    K
Sbjct:   36 KNSPLISNLTLYDIAHTPGVAADLSHIETRAKVTGYLGAEQLPESLKGADVVVIPAGVPRKPGMTRDDLFTTNASIVATLTEACAKHCPEAMICIISNPVNSTIPITSEVFKKHGVYNPNHIFGVTTLDIVRANTFVAELKGLDPARVNVPVVGGHAGKTIIPLISQSTPKVEFPQDQLEALIPRIQDAGTEVVKAKAGAGSATLSMAYAGARFVFSLLDAMNGKEGVIECSFVRSEETESPYFSTPLLLGKNGIEKNLGLGKLTAFEEKLVSEAMGELKASIKKGEDFIKSRK 329
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:MGI Symbol;Acc:MGI:97050])

HSP 1 Score: 385.956 bits (990), Expect = 4.529e-130
Identity = 187/294 (63.61%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AM+CII NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  G+EKNLG+  +T FE+++I +A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMVCIIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVQSKETECTYFSTPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKNMK 338

HSP 2 Score: 52.373 bits (124), Expect = 8.018e-7
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:MGI Symbol;Acc:MGI:97050])

HSP 1 Score: 242.276 bits (617), Expect = 2.208e-76
Identity = 116/169 (68.64%), Postives = 137/169 (81.07%), Query Frame = 1 
Query:  163 TSKKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQ 669
            +++ S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AM+CII NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ
Sbjct:   20 SAQNSPLVSRLTLYDIAHTPGVAADLSHIETRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMVCIIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQ 188
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:MGI Symbol;Acc:MGI:97050])

HSP 1 Score: 147.902 bits (372), Expect = 9.745e-45
Identity = 76/137 (55.47%), Postives = 87/137 (63.50%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFI 579
            K S  V RL LYD+AH  GVAADLSHIET A V G                            MTRDDLFNTNA+IVA L   CAQ CP+AM+CII NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETRANVKG----------------------------MTRDDLFNTNATIVATLTAACAQHCPEAMVCIIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFV 153

HSP 2 Score: 53.1434 bits (126), Expect = 9.745e-45
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Ldhc (lactate dehydrogenase C [Source:MGI Symbol;Acc:MGI:96764])

HSP 1 Score: 66.2402 bits (160), Expect = 3.279e-11
Identity = 79/301 (26.25%), Postives = 131/301 (43.52%), Query Frame = 1 
Query:  187 DRLALYD--VAHVKGVAADLSH----IETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGV-TTLDIVRSNTFIAEAKLLDVTKMSVHVIGGH--------SGVTIIPVISQAI-PSVSFPESEINKLTIRIQ--NAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEF 1035
            D LAL D     ++G A DL H    + TP +V G     +      NS +V+I AG     G TR DL   N +I+  +V G  Q  P   I I+TNPV+    I   ++ K   +   R+ G    LD  R    I E   ++ T     V+G H        SGV +  V  +++ P++     + +   +  Q    G EV+  K   G  + ++  +     + IL  +K    +         + E  F + P +LG +GI   + + ++TA E+ L+KK+  + L N++  ++ 
Sbjct:   46 DELALVDADTNKLRGEALDLLHGSLFLSTPKIVFG-----KDYNVSANSKLVIITAGARMVSGETRLDLLQRNVAIMKAIVPGIVQNSPDCKIIIVTNPVD----ILTYVVWKISGFPVGRVIGSGCNLDSARFRYLIGEKLGVNPTSCHGWVLGEHGDSSVPIWSGVNVAGVTLKSLNPAIGTDSDKEHWKNVHKQVVEGGYEVLNMK---GYTSWAIGLSVTDLARSILKNLKRVHPVTTLVKGFHGIKEEVFLSIPCVLGQSGITDFVKV-NMTAEEEGLLKKS-ADTLWNMQKDLQL 332
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Ldhc (lactate dehydrogenase C [Source:MGI Symbol;Acc:MGI:96764])

HSP 1 Score: 55.4546 bits (132), Expect = 4.413e-8
Identity = 53/167 (31.74%), Postives = 75/167 (44.91%), Query Frame = 1 
Query:  187 DRLALYD--VAHVKGVAADLSH----IETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGV-TTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVIS 666
            D LAL D     ++G A DL H    + TP +V G     +      NS +V+I AG     G TR DL   N +I+  +V G  Q  P   I I+TNPV+    I   ++ K   +   R+ G    LD  R    I E   ++ T     V+G H G + +P+ S
Sbjct:   46 DELALVDADTNKLRGEALDLLHGSLFLSTPKIVFG-----KDYNVSANSKLVIITAGARMVSGETRLDLLQRNVAIMKAIVPGIVQNSPDCKIIIVTNPVD----ILTYVVWKISGFPVGRVIGSGCNLDSARFRYLIGEKLGVNPTSCHGWVLGEH-GDSSVPIWS 202
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|Q5NVR2|MDHM_PONAB (Malate dehydrogenase, mitochondrial OS=Pongo abelii OX=9601 GN=MDH2 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.300e-130
Identity = 187/294 (63.61%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMIC+I NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  GIEKNLG+  +++FE+++I  A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETKATVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTSACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 338

HSP 2 Score: 51.9878 bits (123), Expect = 8.662e-6
Identity = 26/32 (81.25%), Postives = 28/32 (87.50%), Query Frame = 2 
Query:   80 RSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   15 RSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|P40926|MDHM_HUMAN (Malate dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=MDH2 PE=1 SV=3)

HSP 1 Score: 388.267 bits (996), Expect = 3.398e-130
Identity = 187/294 (63.61%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMIC+I NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  TE  +F+ PLLLG  GIEKNLG+  +++FE+++I  A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK 338

HSP 2 Score: 52.373 bits (124), Expect = 5.760e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|P04636|MDHM_RAT (Malate dehydrogenase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Mdh2 PE=1 SV=2)

HSP 1 Score: 387.882 bits (995), Expect = 5.475e-130
Identity = 189/294 (64.29%), Postives = 229/294 (77.89%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMICII+NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + +IEC+FV+S  TE  +F+ PLLLG  G+EKNLG+  +T FE+++I +A+PEL A+IK G +F    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETRANVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVIECSFVQSKETECTYFSTPLLLGKKGLEKNLGIGKITPFEEKMIAEAIPELKASIKKGEDFVKNMK 338

HSP 2 Score: 52.7582 bits (125), Expect = 5.178e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|P00346|MDHM_PIG (Malate dehydrogenase, mitochondrial OS=Sus scrofa OX=9823 GN=MDH2 PE=1 SV=2)

HSP 1 Score: 387.497 bits (994), Expect = 7.744e-130
Identity = 188/294 (63.95%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP AMICII+NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+N F+AE K LD  ++SV VIGGH+G TIIP+ISQ  P V FP+ +++ LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  T+  +F+ PLLLG  GIEKNLG+  ++ FE+++I +A+PEL A+IK G EF    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETRATVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPDAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANAFVAELKGLDPARVSVPVIGGHAGKTIIPLISQCTPKVDFPQDQLSTLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETDCPYFSTPLLLGKKGIEKNLGIGKISPFEEKMIAEAIPELKASIKKGEEFVKNMK 338

HSP 2 Score: 52.7582 bits (125), Expect = 5.413e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSXQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|Q32LG3|MDHM_BOVIN (Malate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=MDH2 PE=1 SV=1)

HSP 1 Score: 386.341 bits (991), Expect = 1.823e-129
Identity = 187/294 (63.61%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V RL LYD+AH  GVAADLSHIET A V GYLGP +L +CLK  DVVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CAQ CP+AMICII+NPVNST+PI AE+ KK G Y+  ++FGVTTLDIVR+N F+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP+ ++  LT RIQ AGTEVV+AKAGAGSATLSMAYAG RFV  ++ AM G + ++EC+FV+S  T+  +F+ PLLLG  GIEKNLG+  ++ FE+++I +A+PEL A+IK G EF    K
Sbjct:   45 KNSPLVSRLTLYDIAHTPGVAADLSHIETRATVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICIISNPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANAFVAELKDLDPARVNVPVIGGHAGKTIIPLISQCTPKVEFPQDQLTTLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETDCPYFSTPLLLGKKGIEKNLGIGKVSPFEEKMIAEAIPELKASIKKGEEFVKNMK 338

HSP 2 Score: 52.7582 bits (125), Expect = 4.998e-6
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 2 
Query:   68 AKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKS 175
            A + RSFST    NAKVAVLGASGGIGQP+SLLLK+
Sbjct:   11 AALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKN 46
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: G4VBJ0 (Malate dehydrogenase OS=Schistosoma mansoni OX=6183 GN=Smp_047370 PE=3 SV=1)

HSP 1 Score: 428.713 bits (1101), Expect = 1.855e-143
Identity = 210/296 (70.95%), Postives = 243/296 (82.09%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
            K+S  + +LALYD+AHVKGVAADLSHIET A VT +LGPGEL ECL  ++VV+IPAG+PRKPGMTRDDLFNTNASIVA+L+  CA+ CPKAMICIITNPVNSTVPIAAEILK+   YD +RLFGVTTLD+VRSNTFIA+AK L V K+S  VIGGHSG+TI+PVISQ  P VSFP+ E  K+T RIQ AGTEVVEAKAGAGSATLSMAYAGVRF   +L AM G   ++ECAFVES+VTE +FF+ PL LG  G+EKN+G+  L  +E EL+KK +PEL ANIK G EFA KY PK
Sbjct:   46 KQSPLISQLALYDIAHVKGVAADLSHIETQAHVTAHLGPGELAECLTGANVVIIPAGMPRKPGMTRDDLFNTNASIVAELIDSCAKNCPKAMICIITNPVNSTVPIAAEILKRHNVYDPKRLFGVTTLDVVRSNTFIAQAKDLAVRKVSCPVIGGHSGITILPVISQCSPHVSFPQDEREKITKRIQEAGTEVVEAKAGAGSATLSMAYAGVRFAVSLLEAMSGRAGVVECAFVESDVTECEFFSTPLALGAEGVEKNMGIGKLNEYEIELLKKLIPELKANIKKGKEFAAKYTPK 341
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A430QMD7 (Malate dehydrogenase OS=Schistosoma bovis OX=6184 GN=DC041_0012974 PE=3 SV=1)

HSP 1 Score: 428.328 bits (1100), Expect = 2.714e-143
Identity = 209/296 (70.61%), Postives = 243/296 (82.09%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
            K+S  + +L+LYD+AHVKGVAADLSHIET A VT +LGPGEL ECL  ++VV+IPAG+PRKPGMTRDDLFNTNASIVA+L+  CA+ CPKAMICIITNPVNSTVPIAAEILK+   YD +RLFGVTTLD+VRSNTFIA+AK L V K+S  VIGGHSG+TI+PVISQ  P VSFP+ E  K+T RIQ AGTEVVEAKAGAGSATLSMAYAGVRF   +L AM G   ++ECAFVES+VTE +FF+ PL LG  G+EKN+G+  L  +E EL+KK +PEL ANIK G EFA KY PK
Sbjct:   46 KQSPLISQLSLYDIAHVKGVAADLSHIETQAQVTAHLGPGELAECLSGANVVIIPAGMPRKPGMTRDDLFNTNASIVAELIDSCAKNCPKAMICIITNPVNSTVPIAAEILKRHNVYDPKRLFGVTTLDVVRSNTFIAQAKDLAVRKVSCPVIGGHSGITILPVISQCSPHVSFPQDEREKITKRIQEAGTEVVEAKAGAGSATLSMAYAGVRFAVSLLEAMSGRAGVVECAFVESDVTECEFFSTPLALGAEGVEKNMGIGKLNEYEIELLKKLIPELKANIKKGKEFAAKYTPK 341
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A183LL11 (Malate dehydrogenase OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS4486 PE=3 SV=1)

HSP 1 Score: 428.328 bits (1100), Expect = 2.714e-143
Identity = 209/296 (70.61%), Postives = 243/296 (82.09%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
            K+S  + +L+LYD+AHVKGVAADLSHIET A VT +LGPGEL ECL  ++VV+IPAG+PRKPGMTRDDLFNTNASIVA+L+  CA+ CPKAMICIITNPVNSTVPIAAEILK+   YD +RLFGVTTLD+VRSNTFIA+AK L V K+S  VIGGHSG+TI+PVISQ  P VSFP+ E  K+T RIQ AGTEVVEAKAGAGSATLSMAYAGVRF   +L AM G   ++ECAFVES+VTE +FF+ PL LG  G+EKN+G+  L  +E EL+KK +PEL ANIK G EFA KY PK
Sbjct:   46 KQSPLISQLSLYDIAHVKGVAADLSHIETQAQVTAHLGPGELAECLSGANVVIIPAGMPRKPGMTRDDLFNTNASIVAELIDSCAKNCPKAMICIITNPVNSTVPIAAEILKRHNVYDPKRLFGVTTLDVVRSNTFIAQAKDLAVRKVSCPVIGGHSGITILPVISQCSPHVSFPQDEREKITKRIQEAGTEVVEAKAGAGSATLSMAYAGVRFAVSLLEAMSGRAGVVECAFVESDVTECEFFSTPLALGAEGVEKNMGIGKLNEYEIELLKKLIPELKANIKKGKEFAAKYTPK 341
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: G7YWM7 (Malate dehydrogenase OS=Clonorchis sinensis OX=79923 GN=CLF_112591 PE=3 SV=1)

HSP 1 Score: 424.476 bits (1090), Expect = 5.628e-142
Identity = 209/336 (62.20%), Postives = 256/336 (76.19%), Query Frame = 1 
Query:   64 ISKSLSIIFNMSSTKC*SCSFRSKWWNWTANV-----ITSKKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
             S++   + + + +KC S S +      +  +     +  K+S  V  LALYD+AHVKGVAADLSHIET A VTG+ GP +L ECL  ++VV+IPAGVPRKPGMTRDDLFNTNASIVA LVH CA  CPKAMICI+TNPVNSTVPIAAEI+K+ G +D  RLFGVTTLDI+RSNTFIAEAK LDV K+S  VIGGHSG+TI+PVISQ  P+VSFP++E  +LT RIQNAGTEVVEAKAGAGSATLSMAYAGVRF   ++ AM G + ++EC FV   V+E +FFA P+ LG NG+EKN+G+  L  +E +L++K +PEL  NIK G EFA  +KP+
Sbjct:    2 FSRAALSVRHFTGSKCFSTSAKVALLGASGGIGQPTALLLKQSPLVSHLALYDIAHVKGVAADLSHIETKARVTGHDGPAQLAECLTGAEVVIIPAGVPRKPGMTRDDLFNTNASIVAQLVHACALNCPKAMICIVTNPVNSTVPIAAEIMKRHGVFDPLRLFGVTTLDIIRSNTFIAEAKGLDVQKVSCPVIGGHSGITILPVISQCSPTVSFPQNEREQLTSRIQNAGTEVVEAKAGAGSATLSMAYAGVRFATSLMEAMSGRQGVVECTFVHGEVSECEFFAAPIALGVNGVEKNMGIGKLNEYEIQLLQKLIPELQKNIKRGKEFAATFKPQ 337
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: Q4VVC2 (Malate dehydrogenase OS=Clonorchis sinensis OX=79923 PE=2 SV=1)

HSP 1 Score: 424.476 bits (1090), Expect = 8.224e-142
Identity = 204/296 (68.92%), Postives = 241/296 (81.42%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
            K+S  V  LALYD+AHVKGVAADLSHIET A VTG+ GP +L ECL  ++VV+IPAGVPRKPGMTRDDLFNTNASIVA LVH CA  CPKAMICI+TNPVNSTVPIAAEI+K+ G +D  RLFGVTTLDI+RSNTFIAEAK LDV K+S  VIGGHSG+TI+PVISQ  P+VSFP++E  +LT RIQNAGTEVVEAKAGAGSATLSMAYAGVRF   ++ AM G + ++EC FV   V+E +FFA P+ LG NG+EKN+G+  L  +E +L++K +PEL  NIK G EFA  +KP+
Sbjct:   46 KQSPLVSHLALYDIAHVKGVAADLSHIETKARVTGHDGPAQLAECLTGAEVVIIPAGVPRKPGMTRDDLFNTNASIVAQLVHACALNCPKAMICIVTNPVNSTVPIAAEIMKRHGVFDPLRLFGVTTLDIIRSNTFIAEAKGLDVQKVSCPVIGGHSGITILPVISQCSPTVSFPQNEREQLTSRIQNAGTEVVEAKAGAGSATLSMAYAGVRFATSLMEAMSGRQGVVECTFVHGEVSECEFFAAPIALGVNGVEKNMGIGKLNEYEIQLLQKLIPELQKNIKRGKEFAATFKPQ 341
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh2 (malate dehydrogenase 2 [Source:NCBI gene;Acc:103036822])

HSP 1 Score: 387.882 bits (995), Expect = 6.213e-131
Identity = 190/294 (64.63%), Postives = 230/294 (78.23%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V  L+LYD+AH  GVAADLSHIET A VTGY+GP +L   LK  +VVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CA+ACP+AMICII NPVNSTVPI +E++KK G Y+  R+FGVTTLDIVR+N F+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FPE +++ LT+RIQ+AGTEVV+AKAGAGSATLSMAYAG RF   +L AM G + ++ECAFV S  TE K+F+ PLLLG NGIEKNLG+  L+ FEK+L+ +A+ EL  +IK G +FA   K
Sbjct:   44 KNSPLVSELSLYDIAHTPGVAADLSHIETRAKVTGYIGPDQLEAALKGCEVVVIPAGVPRKPGMTRDDLFNTNATIVATLSDACARACPEAMICIIANPVNSTVPITSEMMKKHGVYNPNRVFGVTTLDIVRANAFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVEFPEDQLSALTVRIQDAGTEVVKAKAGAGSATLSMAYAGARFTFSLLDAMNGKEGVVECAFVRSEETECKYFSTPLLLGKNGIEKNLGVGTLSVFEKKLVAEALGELKGSIKKGEDFATNLK 337
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Sea Lamprey
Match: mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:ZFIN;Acc:ZDB-GENE-040426-2143])

HSP 1 Score: 309.686 bits (792), Expect = 4.189e-101
Identity = 156/299 (52.17%), Postives = 208/299 (69.57%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKP---GMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVT--KMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K ++ V RLALYD+A   GVA DL HI + A V+ + G  +L E L  S +VV+PAGVPRKP   GMTR+DLF  NA IVA+L   CA  CP+A++CII+N VNS VPIA+EI KK G YD +R+FGVTTLD+VR++ F+A+ K L +    +   V+GGH+G TIIP++SQ++P V   + +I  LT R+Q AGTEVV+AK G GSATLSMA+AG RFV  +L A  G+K ++ECAFV S  TEA + + PLLLG  G+E+++GL      E+ +    +PEL A I++G EFA  +K
Sbjct:   45 KMNARVSRLALYDIARAHGVAVDLGHINSHASVSAHQGANQLSEALDGSHLVVVPAGVPRKPAPEGMTREDLFTMNAHIVAELAEACAITCPQALLCIISNLVNSMVPIASEIFKKHGVYDPKRIFGVTTLDVVRASVFLADLKGLSMWCGWLFCPVVGGHAGKTIIPLLSQSLPKVDLGQDQIEMLTSRLQEAGTEVVKAKVGEGSATLSMAHAGARFVSSLLEASDGHKGLVECAFVRSEETEAAYLSTPLLLGKRGVERSMGLQK-RGVERSMGLGPLPELKAAIRDGEEFARNFK 342
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Yeast
Match: MDH1 (Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated [Source:SGD;Acc:S000001568])

HSP 1 Score: 265.003 bits (676), Expect = 3.528e-84
Identity = 148/294 (50.34%), Postives = 205/294 (69.73%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYL--GPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEA---KFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEF 1035
            K +  V  L LYD+   KGVA DLSHI T +VV G+    P  L   LK++D+V+IPAGVPRKPGMTRDDLF  NASIV DL    A++ P A I +I+NPVNSTVPI A++LK  G Y+ ++LFGVTTLD +R+  FI+E +  D T+  V+VIGGHSG+TIIP+ISQ    +   + + ++L  RIQ  G EVV+AK GAGSATLSMA+AG +F   +L+  KG +++IE +FV+S + ++   +FFA+P+ LGP+GIEK   + +L++ E+E+++K    L  NI+ G+ F
Sbjct:   38 KLNHKVTDLRLYDLKGAKGVATDLSHIPTNSVVKGFTPEEPDGLNNALKDTDMVLIPAGVPRKPGMTRDDLFAINASIVRDLAAATAESAPNAAILVISNPVNSTVPIVAQVLKNKGVYNPKKLFGVTTLDSIRAARFISEVENTDPTQERVNVIGGHSGITIIPLISQTNHKL-MSDDKRHELIHRIQFGGDEVVKAKNGAGSATLSMAHAGAKFANAVLSGFKGERDVIEPSFVDSPLFKSEGIEFFASPVTLGPDGIEKIHPIGELSSEEEEMLQKCKETLKKNIEKGVNF 330

HSP 2 Score: 45.8246 bits (107), Expect = 9.955e-6
Identity = 25/47 (53.19%), Postives = 34/47 (72.34%), Query Frame = 2 
Query:   53 ILLKLAKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLKSHQQLID 193
            +L ++AK  R+FS+  A   KV VLGA GGIGQP+SLLLK + ++ D
Sbjct:    1 MLSRVAK--RAFSSTVANPYKVTVLGAGGGIGQPLSLLLKLNHKVTD 45
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Yeast
Match: MDH3 (Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle [Source:SGD;Acc:S000002236])

HSP 1 Score: 199.904 bits (507), Expect = 2.272e-59
Identity = 132/315 (41.90%), Postives = 178/315 (56.51%), Query Frame = 1 
Query:  184 VDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLL---------DVTKM--SVHVIGGHSGVTIIPVISQAIPSVSFP-ESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIE--CAFVE----SNVTEAK---------FFANPLLLGPNG----IEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEF 1035
            V  LALYD+   +G+  DLSHI T +   GY     +   L N+ VV+IPAGVPRKPG+TRDDLF  NA IV  LV    +  P A I +I+NPVNS VPIA E LKK G +    + GVT LD+VR+ TF+ +  +L         D T M   V VIGGHSG TIIP+I+    S+ F  + +      R+Q  G E+V+AK GAGSATLSMA+AG +F + +L +    K   E   AFV      N  +A+         +F+ P++L  NG    I+ ++ L  L+  E++L+  A+ EL  NI+ G  F
Sbjct:   27 VSELALYDIRAAEGIGKDLSHINTNSSCVGY-DKDSIENTLSNAQVVLIPAGVPRKPGLTRDDLFKMNAGIVKSLVTAVGKFAPNARILVISNPVNSLVPIAVETLKKMGKFKPGNVMGVTNLDLVRAETFLVDYLMLKNPKIGQEQDKTTMHRKVTVIGGHSGETIIPIITDK--SLVFQLDKQYEHFIHRVQFGGDEIVKAKQGAGSATLSMAFAGAKFAEEVLRSFHNEKPETESLSAFVYLPGLKNGKKAQQLVGDNSIEYFSLPIVL-RNGSVVSIDTSV-LEKLSPREEQLVNTAVKELRKNIEKGKSF 336
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Yeast
Match: MDH2 (Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 [Source:SGD;Acc:S000005486])

HSP 1 Score: 190.274 bits (482), Expect = 2.849e-55
Identity = 131/329 (39.82%), Postives = 183/329 (55.62%), Query Frame = 1 
Query:  166 SKKSSAVDRLALYDVAH--VKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQAC--PKAMICIITNPVNSTVPI-AAEILK------KAGCYDARRLFGVTTLDIVRSNTFIAEAKLLD-----VTKM-SVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFV----ESN--------------VTEAKFFANPLLLGPNG---IEKNLGLPDLTAFEK-ELIKKAMPELLANIKNGIEF 1035
            S +S     LALYDV    + GV ADLSHI+TP  V+ +   G +  CL N+ +VVIPAGVPRKPGMTRDDLFN NA I++ L    A+ C   K  + +I+NPVNS VP+  + ILK       +G    RR+ GVT LDIVR++TF+ E  +       V  M  V VIGGHSG TIIP+ SQ+       E ++  L  R+Q  G EVV+AK G GSATLSMA+AG + V + ++ + GN   I   +     ++N              V  A +FA PL +   G   ++ ++ +  +   E+ +++   + +L  NI  G+EF
Sbjct:   44 SNRSVTHIHLALYDVNQEAINGVTADLSHIDTPISVSSHSPAGGIENCLHNASIVVIPAGVPRKPGMTRDDLFNVNAGIISQLGDSIAECCDLSKVFVLVISNPVNSLVPVMVSNILKNHPQSRNSGI--ERRIMGVTKLDIVRASTFLREINIESGLTPRVNSMPDVPVIGGHSGETIIPLFSQSNFLSRLNEDQLKYLIHRVQYGGDEVVKAKNGKGSATLSMAHAGYKCVVQFVSLLLGNIEQIHGTYYVPLKDANNFPIAPGADQLLPLVDGADYFAIPLTITTKGVSYVDYDI-VNRMNDMERNQMLPICVSQLKKNIDKGLEF 369
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Match: EDO30176 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:A7T1J0])

HSP 1 Score: 375.555 bits (963), Expect = 1.616e-126
Identity = 179/292 (61.30%), Postives = 230/292 (78.77%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANK 1044
            K+S  +  LALYD+ +  GVAADLSHI T A VT + GP +L   L+   VV IPAGVPRKPGMTRDDLFNTNASIV +L   CA+ CPKA+ICII+NPVNSTVPIA+E+ KKAG YD  R+ GVTTLDIVR++TF+AEAK LDV  + + VIGGHSGVTI+P++SQ  P+V+F + E+ KLT RIQNAGTEVV AKAGAGSATLSMAYAG  FV  ++ A+ G K++++CAF++S++TEA +FA P++LG NG+EKNLG+  L+ +E++ + + +PELL NIK G +F N+
Sbjct:   50 KQSPLISHLALYDIVNTPGVAADLSHISTRAKVTSHQGPDDLKAALEGCSVVAIPAGVPRKPGMTRDDLFNTNASIVKNLSEACAKHCPKAIICIISNPVNSTVPIASEVYKKAGVYDPGRILGVTTLDIVRAHTFVAEAKGLDVNNIKIPVIGGHSGVTILPLLSQTTPNVTFTQDELEKLTDRIQNAGTEVVNAKAGAGSATLSMAYAGKEFVHSVIEALNGKKDVVQCAFIKSDLTEAGYFATPVVLGTNGVEKNLGMGKLSDYEQKKMGEVIPELLKNIKKGEDFVNQ 341
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Match: EDO33958 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:A7SQS1])

HSP 1 Score: 313.538 bits (802), Expect = 1.283e-103
Identity = 156/274 (56.93%), Postives = 197/274 (71.90%), Query Frame = 1 
Query:  223 GVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANK 1044
            GVAADLSHI T A VT + GP +L   L+   VV IPAGVPRKPGMTRDDLFNTNASIV +L   CA+ CPKA+ICII+NPVNSTVPIA+E+ KKAG YD  R+ GVTTLDIVR++TF+AEAK                            P V+F + ++ KLT RIQNAGTEVV AKAGAGSATLSMAYAG  FV  ++ A+ G K++++CAF++S++TEA +FA P+LLG NG+EKNLG+  L+ +E++ + + +PELL NIK G +F N+
Sbjct:    7 GVAADLSHISTRAKVTSHQGPDDLKAALEGCSVVAIPAGVPRKPGMTRDDLFNTNASIVKNLSEACAKHCPKAIICIISNPVNSTVPIASEVYKKAGVYDPARILGVTTLDIVRAHTFVAEAK--------------------------TTPCVNFTQDDLEKLTDRIQNAGTEVVNAKAGAGSATLSMAYAGKEFVHSVIEALNGKKDVVQCAFIKSHLTEAGYFATPVLLGTNGVEKNLGMGKLSDYEQKKMGEVIPELLKNIKKGEDFVNQ 254
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdh2 (malate dehydrogenase 2, NAD (mitochondrial) [Source:ZFIN;Acc:ZDB-GENE-040426-2143])

HSP 1 Score: 381.333 bits (978), Expect = 5.386e-128
Identity = 188/294 (63.95%), Postives = 228/294 (77.55%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYK 1050
            K S  V  L+LYD+AH  GVAADLSHIET A VTGY+GP +L   L+  +VVVIPAGVPRKPGMTRDDLFNTNA+IVA L   CA+ CP+AMICII NPVNST+PI +E++KK G Y+  R+FGVTTLDIVR+NTF+AE K LD  +++V VIGGH+G TIIP+ISQ  P V FP  +++ LT RIQ AGTEVV+AKAGAGSATLSMAYAG RF   +L AM G + ++ECAFV S  TE K+F+ PLLLG +GIEKNLGL  L+AFE++L+  A+ EL A+IK G +FA   K
Sbjct:   71 KNSPLVSHLSLYDIAHTPGVAADLSHIETRAQVTGYMGPDQLDAALQGCEVVVIPAGVPRKPGMTRDDLFNTNATIVATLADACARNCPEAMICIIANPVNSTIPITSEVMKKHGVYNPNRVFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVEFPADQLSALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFTFSVLDAMNGKEGVVECAFVRSEETECKYFSTPLLLGKSGIEKNLGLGKLSAFEEKLVADAIGELKASIKKGEDFAANMK 364

HSP 2 Score: 51.6026 bits (122), Expect = 1.344e-6
Identity = 33/69 (47.83%), Postives = 38/69 (55.07%), Query Frame = 2 
Query:    2 YLQILKDLKKYFSMNFSILLKLAKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLK-----SHQQLID 193
            + +   D +K FS      +  A   R  ST    NAKVAVLGASGGIGQP+SLLLK     SH  L D
Sbjct:   18 WREKCSDRRKMFSRAVRPTVSFA---RCLSTSSQNNAKVAVLGASGGIGQPLSLLLKNSPLVSHLSLYD 83
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BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000008924.1 (SMESG000008924.1)

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 1056
            KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSV VIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK
Sbjct:   44 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVPVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKYKPK 339

HSP 2 Score: 85.1149 bits (209), Expect = 9.947e-18
Identity = 43/44 (97.73%), Postives = 43/44 (97.73%), Query Frame = 2 
Query:   41 MNFSILLKLAKVSRSFSTCQALNAKVAVLGASGGIGQPMSLLLK 172
            MNFSILLKLAKV RSFSTCQALNAKVAVLGASGGIGQPMSLLLK
Sbjct:    1 MNFSILLKLAKVFRSFSTCQALNAKVAVLGASGGIGQPMSLLLK 44
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000035456.1 (SMESG000035456.1)

HSP 1 Score: 323.939 bits (829), Expect = 3.436e-106
Identity = 155/293 (52.90%), Postives = 214/293 (73.04%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLGPGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKY 1047
            KK+  ++ LALYD+ +VKGVA DLSHIE    VT + G  EL  CL  SD+VVIPAG+PR PGM+R+DLF +NA+I+A+L    A+ CP A+IC+ITNP+NS VP+ AEIL     +D+++LFG+TTL  +R+ TFIA+AK +D+TK+ V VIGGHSG TIIPVISQ+ P V FP  E N +  +I+NAG+E+VEAKAG GSATLSM++A  RF+  ++ A  G   +IE A+V+S+ TE ++FA+ LL G  G+E+NLG+  +T FE  L+K A+P +  +I+ G ++A  Y
Sbjct:   43 KKTPLIEELALYDIKNVKGVAVDLSHIERKGKVTAFEGKEELGACLDKSDIVVIPAGLPRNPGMSRNDLFKSNAAIIAELTVSIAKHCPNALICLITNPLNSLVPLMAEILNHFNVFDSKKLFGITTLSTIRAITFIAQAKNIDITKLHVPVIGGHSGSTIIPVISQSAPHVQFPLEERNMIVDKIRNAGSEIVEAKAGQGSATLSMSWAAYRFINSLIEARGGRTGVIESAYVKSDETECEYFASSLLFGRQGVERNLGIGQMTEFEISLLKAAIPAIKRDIEVGKKYAKSY 335
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000008151.1 (SMESG000008151.1)

HSP 1 Score: 292.738 bits (748), Expect = 4.478e-94
Identity = 155/294 (52.72%), Postives = 202/294 (68.71%), Query Frame = 1 
Query:  169 KKSSAVDRLALYDVAHVKGVAADLSHIETPAVVTGYLG-PGELLECLKNSDVVVIPAGVPRKPGMTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKKAGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIPVISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVRFVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPDLTAFEKELIKKAMPELLANIKNGIEFANKY 1047
            K S +V  LALYD+  V GVAADLSHI   A +TG    P  L EC+K+S +VVI AG  R   MTR DLF TNASIV+ L H CA   P+ M+CI+TNP++S VP+ A+I K    YD  RLFGVT+L+ VR++TF+A+AK L  TK+ V VIGG+S  T+IP+ISQ IP +SFP  +  +LT RIQ+AGTE+ EAKAG GSATLS AY+  +F+  ++  M G K +++CA+VES     +FFA P++LG NGI K +G   L  +E  LI+ A+PEL  +IK G EFA +Y
Sbjct:   44 KDSISVTELALYDIKSVAGVAADLSHISKRAKITGTSSSPEGLSECIKDSKIVVIVAGRSRNLDMTRFDLFTTNASIVSSLAHQCAIVNPQCMLCIVTNPIDSLVPMVAQIFKSLSVYDPCRLFGVTSLNSVRASTFVAQAKGLIGTKLYVPVIGGNSENTLIPLISQCIPPLSFPPKQRIELTRRIQSAGTEINEAKAGKGSATLSTAYSTFKFITSLIDGMDGKKGVVQCAYVESTRGPCEFFATPIVLGMNGIHKIIGAGRLIDYELSLIEAAIPELQESIKTGKEFAAQY 337
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000011541.1 (SMESG000011541.1)

HSP 1 Score: 44.669 bits (104), Expect = 7.368e-8
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 3 
Query: 1656 AHIFNHCSKTQNARGNKQ-QCT*TC*RFLYFQKFLVDIEKSSPGVTEPLRP 1805
            AHIFNHC++TQNAR NK      T   +L  + F VD+E +  G+   LRP
Sbjct:  743 AHIFNHCTQTQNARRNKHNNVMKTTSDYLISKGFSVDVECTPQGIDIRLRP 793

HSP 2 Score: 31.187 bits (69), Expect = 7.368e-8
Identity = 15/45 (33.33%), Postives = 29/45 (64.44%), Query Frame = 2 
Query: 1532 NKNFVCGLIVENNMLSLKYSP*RHKLKQNMLYRLCNTDWETRTYF 1666
            +K ++  +   NNM++L +S  R+K K N++ RLC+ + ET+ + 
Sbjct:  701 DKQYLFLIKARNNMITLNFSTNRNKNKMNIMCRLCHKEPETQAHI 745
The following BLAST results are available for this feature:
BLAST of Malate dehydrogenase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MDH27.076e-13163.61malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGN... [more]
MDH21.501e-10054.08malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGN... [more]
MDH22.652e-9661.04malate dehydrogenase 2 [Source:HGNC Symbol;Acc:HGN... [more]
LDHAL6A5.042e-1028.04lactate dehydrogenase A like 6A [Source:HGNC Symbo... [more]
LDHAL6A5.042e-1028.04lactate dehydrogenase A like 6A [Source:HGNC Symbo... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdh-21.880e-11560.00Probable malate dehydrogenase, mitochondrial [Sou... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Mdh21.363e-12360.82gene:FBgn0262559 transcript:FBtr0083563[more]
CG107491.173e-9249.32gene:FBgn0036328 transcript:FBtr0075871[more]
CG107482.215e-8947.93gene:FBgn0036327 transcript:FBtr0075900[more]
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BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdh23.923e-12862.93malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sssca11.050e-12964.29Sjogren syndrome/scleroderma autoantigen 1 [Source... [more]
sssca11.213e-12964.29Sjogren syndrome/scleroderma autoantigen 1 [Source... [more]
sssca11.546e-12964.29Sjogren syndrome/scleroderma autoantigen 1 [Source... [more]
sssca12.288e-12964.29Sjogren syndrome/scleroderma autoantigen 1 [Source... [more]
sssca12.302e-12964.29Sjogren syndrome/scleroderma autoantigen 1 [Source... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mdh24.529e-13063.61malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
Mdh22.208e-7668.64malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
Mdh29.745e-4555.47malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
Ldhc3.279e-1126.25lactate dehydrogenase C [Source:MGI Symbol;Acc:MGI... [more]
Ldhc4.413e-831.74lactate dehydrogenase C [Source:MGI Symbol;Acc:MGI... [more]
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BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5NVR2|MDHM_PONAB2.300e-13063.61Malate dehydrogenase, mitochondrial OS=Pongo abeli... [more]
sp|P40926|MDHM_HUMAN3.398e-13063.61Malate dehydrogenase, mitochondrial OS=Homo sapien... [more]
sp|P04636|MDHM_RAT5.475e-13064.29Malate dehydrogenase, mitochondrial OS=Rattus norv... [more]
sp|P00346|MDHM_PIG7.744e-13063.95Malate dehydrogenase, mitochondrial OS=Sus scrofa ... [more]
sp|Q32LG3|MDHM_BOVIN1.823e-12963.61Malate dehydrogenase, mitochondrial OS=Bos taurus ... [more]
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BLAST of Malate dehydrogenase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4VBJ01.855e-14370.95Malate dehydrogenase OS=Schistosoma mansoni OX=618... [more]
A0A430QMD72.714e-14370.61Malate dehydrogenase OS=Schistosoma bovis OX=6184 ... [more]
A0A183LL112.714e-14370.61Malate dehydrogenase OS=Schistosoma margrebowiei O... [more]
G7YWM75.628e-14262.20Malate dehydrogenase OS=Clonorchis sinensis OX=799... [more]
Q4VVC28.224e-14268.92Malate dehydrogenase OS=Clonorchis sinensis OX=799... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdh26.213e-13164.63malate dehydrogenase 2 [Source:NCBI gene;Acc:10303... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdh24.189e-10152.17malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
MDH13.528e-8450.34Mitochondrial malate dehydrogenase; catalyzes inte... [more]
MDH32.272e-5941.90Peroxisomal malate dehydrogenase; catalyzes interc... [more]
MDH22.849e-5539.82Cytoplasmic malate dehydrogenase; one of three iso... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO301761.616e-12661.30Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
EDO339581.283e-10356.93Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdh25.386e-12863.95malate dehydrogenase 2, NAD (mitochondrial) [Sourc... [more]
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BLAST of Malate dehydrogenase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000008924.19.947e-1899.66SMESG000008924.1[more]
SMESG000035456.13.436e-10652.90SMESG000035456.1[more]
SMESG000008151.14.478e-9452.72SMESG000008151.1[more]
SMESG000011541.17.368e-847.06SMESG000011541.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005321 ID=SMED30005321|Name=Malate dehydrogenase|organism=Schmidtea mediterranea sexual|type=transcript|length=1805bp
CTATTTACAAATATTAAAAGATTTAAAAAAATATTTTTCAATGAACTTTT
CTATATTACTCAAATTAGCAAAAGTCTCTCGATCATTTTCAACATGTCAA
GCACTAAATGCTAAAGTTGCAGTTTTAGGAGCAAGTGGTGGAATTGGACA
GCCAATGTCATTACTTCTAAAAAGTCATCAGCAGTTGATAGATTAGCTTT
GTATGATGTTGCACATGTCAAGGGTGTTGCTGCAGATTTAAGTCATATTG
AAACTCCTGCTGTAGTTACTGGATATCTGGGTCCAGGAGAATTGCTTGAA
TGTTTGAAAAATTCTGATGTTGTAGTTATACCAGCAGGAGTTCCTAGAAA
ACCTGGAATGACACGAGATGATTTATTTAATACGAATGCATCAATTGTTG
CGGATCTTGTACATGGATGTGCTCAAGCATGCCCAAAAGCTATGATTTGC
ATTATCACAAATCCAGTTAATAGTACAGTTCCAATTGCAGCGGAAATTTT
AAAGAAAGCTGGTTGTTATGACGCTAGAAGACTTTTTGGGGTAACAACTT
TGGATATTGTTAGGTCAAATACTTTTATCGCAGAAGCCAAGCTATTAGAC
GTGACAAAAATGTCAGTGCATGTTATTGGTGGGCATTCTGGAGTCACCAT
AATTCCGGTAATATCTCAGGCTATTCCTTCTGTTTCTTTCCCTGAGAGTG
AAATCAACAAGTTAACAATAAGAATTCAAAATGCCGGAACCGAAGTTGTT
GAAGCTAAAGCTGGGGCTGGTTCAGCTACTTTGAGCATGGCCTATGCTGG
AGTTCGATTCGTCAAGCGGATCCTGGCGGCTATGAAAGGAAATAAAAACA
TCATTGAATGCGCATTTGTAGAATCAAATGTCACGGAAGCCAAATTCTTT
GCCAATCCACTATTGCTTGGGCCGAACGGAATTGAGAAAAACTTGGGATT
GCCTGATTTGACAGCTTTTGAAAAGGAACTGATCAAGAAAGCTATGCCAG
AGTTACTAGCCAATATCAAGAACGGAATCGAATTTGCAAATAAGTATAAA
CCAAAATAAACCGATTATTATGCATATACTATTTGTTTAGTGATTTTACC
AGTGAACTTTTGTAACATATAAAATGTTTTTGTTCTTTGTTTTATTAATA
ATCAATTTTATATATAGGATAAATTGTGTGAATTGTGGAAAAGGATAAAT
TTTAGCCAATTAGGATGAAGTGTATTATGAGCGTTCCTATTGGTTAAAGT
AAAATATCAATAAAATAATCGGAATAGAACAAACTATTCACATTTCATAA
ATGTTGGGTTCTAGTCTGATGTATAAGACTTATAAATGTAAAAATACAAA
TTAATAAAATAAATTTTTAATTCAGTTGAATCAGTGTTTTTGACTGATGT
AAATTTATGTACAGCACATAATTTTTTTAAAAAAAAATTTAAGCTCAAGT
TATTCAGTGACAATGGGTGACAACCAAAATTAATTTGTGTAACAGTATGA
AAAATGAATTATTTTTTGAATTTATGTTCTGAATAAGAATTTTGTTTGTG
GGCTTATAGTAGAGAATAATATGCTAAGTCTTAAATATAGTCCATAAAGA
CATAAACTAAAACAAAACATGCTGTATAGATTATGTAATACTGATTGGGA
GACTCGCACATATTTTTAATCATTGTAGTAAAACACAAAATGCACGCGGA
AATAAACAACAATGTACTTGAACATGTTAAAGATTTCTTTATTTCCAAAA
ATTTTTAGTCGATATTGAAAAAAGCTCTCCTGGGGTGACTGAGCCATTGA
GACCT
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protein sequence of SMED30005321-orf-1

>SMED30005321-orf-1 ID=SMED30005321-orf-1|Name=SMED30005321-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp
MTRDDLFNTNASIVADLVHGCAQACPKAMICIITNPVNSTVPIAAEILKK
AGCYDARRLFGVTTLDIVRSNTFIAEAKLLDVTKMSVHVIGGHSGVTIIP
VISQAIPSVSFPESEINKLTIRIQNAGTEVVEAKAGAGSATLSMAYAGVR
FVKRILAAMKGNKNIIECAFVESNVTEAKFFANPLLLGPNGIEKNLGLPD
LTAFEKELIKKAMPELLANIKNGIEFANKYKPK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036291NAD(P)-bd_dom_sf
IPR016040NAD(P)-bd_dom
IPR001557L-lactate/malate_DH
IPR001236Lactate/malate_DH_N
IPR015955Lactate_DH/Glyco_Ohase_4_C
IPR022383Lactate/malate_DH_C
IPR001252Malate_DH_AS
Vocabulary: biological process
TermDefinition
GO:0019752carboxylic acid metabolic process
GO:0055114oxidation-reduction process
GO:0005975carbohydrate metabolic process
GO:0006108malate metabolic process
GO:0006099tricarboxylic acid cycle
Vocabulary: molecular function
TermDefinition
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0016491oxidoreductase activity
GO:0003824catalytic activity
GO:0016615malate dehydrogenase activity
GO:0030060L-malate dehydrogenase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 29..65
e-value: 4.1E-7
score: 32.0
NoneNo IPR availablePANTHERPTHR11540MALATE AND LACTATE DEHYDROGENASEcoord: 24..65
NoneNo IPR availablePANTHERPTHR11540:SF55coord: 24..65
IPR001236Lactate/malate dehydrogenase, N-terminalPFAMPF00056Ldh_1_Ncoord: 37..64
e-value: 2.2E-4
score: 21.3
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 36..63