SMED30005308

Overview


Name	SMED30005308
Smed ID	SMED30005308
Length (bp)	916

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005308 (SMED30005308) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005308 (SMED30005308) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005308

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30005308	h1SMcG0012270	dd_Smed_v4_68524_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30005308	h1SMcG0012270	dd_Smed_v4_68524_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30005308	h1SMcG0012270	dd_Smed_v6_68524_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
photoreceptor neuron	SMED30005308	h1SMcG0012270	SMED30005308	smed_20140614	PMID:22884275 Lapan et al., 2012

Note: Hover over icons to view figure legend

Alignments

SMED30005308 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000126:139055..149457 -	4434

Homology

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BLAST of SMED30005308 vs. Ensembl Human
Match: USH1G (USH1 protein network component sans [Source:HGNC Symbol;Acc:HGNC:16356])

HSP 1 Score: 107.457 bits (267), Expect = 1.117e-25
Identity = 53/147 (36.05%), Postives = 88/147 (59.86%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    ELL  A+  E  + DE+GM    WAAY G+LE L+L++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A +  ++E   +LD+ A ++ + +PK V +++ K  ++A +R++   K +
Sbjct:    8 AARDGYLELLKEATRKELNAPDEDGMTPTLWAAYHGNLESLRLIVSRGGDPDKCDIWGNTPLHLAASNGHLHCLSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKLQ 154

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BLAST of SMED30005308 vs. Ensembl Human
Match: ANKS4B (ankyrin repeat and sterile alpha motif domain containing 4B [Source:HGNC Symbol;Acc:HGNC:26795])

HSP 1 Score: 95.1301 bits (235), Expect = 1.746e-21
Identity = 55/154 (35.71%), Postives = 80/154 (51.95%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A   S  ELL  A+  +    DE+GM     AAY G+LE L+++ SRG    + D  G T LH A    H   V +LVN+G  I+ LDND  +PL  A   +  E  + LD  A  +   +PK V R++ + QK A +++K  E+ +
Sbjct:    1 MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDRCDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQ 154

[Close]

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A   S  ELL  A+  +    DE+GM     AAY G+LE L+++ SRG    + D  G T LH A    H   V +LVN+G  I+ LDND  +PL  A   +  E  + LD  A  +   +PK V R++ + QK A +++K  E+ +
Sbjct:    1 MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDRCDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQ 154

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BLAST of SMED30005308 vs. Ensembl Human
Match: EHMT2 (euchromatic histone lysine methyltransferase 2 [Source:HGNC Symbol;Acc:HGNC:14129])

HSP 1 Score: 64.3142 bits (155), Expect = 9.335e-11
Identity = 33/91 (36.26%), Postives = 53/91 (58.24%), Query Frame = 2

Query:  125 IHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +H AA  G +EI  ++L  G +I  +D   RT L  AV+ NH+E+ RY+V  G C+++ + D ++ L  A    N+E+ S L +  Q +VN
Sbjct:  367 LHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVN 457

[Close]

Query:  125 IHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +H AA  G +EI  ++L  G +I  +D   RT L  AV+ NH+E+ RY+V  G C+++ + D ++ L  A    N+E+ S L +  Q +VN
Sbjct:  367 LHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVN 457

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BLAST of SMED30005308 vs. Ensembl Celegans
Match: T28D6.4 (pep chromosome:WBcel235:III:11360456:11366214:1 gene:WBGene00012124.1 transcript:T28D6.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T28D6.4)

HSP 1 Score: 60.4622 bits (145), Expect = 6.589e-10
Identity = 30/103 (29.13%), Postives = 57/103 (55.34%), Query Frame = 2

Query:   62 ELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            + L+  + ++ +++D NG  ++ WAA  G  E+++ + S G      DN+G   LH+AV  +HVE+V++L N    +   D     P+ +A  N N+++  +L
Sbjct:  644 QFLTKTAKADVDAIDNNGRTALQWAAINGHPEVVEFLCSVGSDEGHKDNDGAVALHYAVTHDHVELVKHLTNQHT-VEAKDRYGQHPMMIAAANGNLKVLEYL 745

HSP 2 Score: 51.6026 bits (122), Expect = 4.189e-7
Identity = 44/149 (29.53%), Postives = 64/149 (42.95%), Query Frame = 2

Query:   38 NAVKQ---SNTELLSMASSSECESVDENGMNSIHWAAYLGD--LEI------------------LKLVLSRG-------------CSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDN 376
            N  KQ   S T  L +  +++C+  D NG  ++H AAY GD  L++                  L L  S+G               +  IDNNGRT L  A I  H E+V +L + G    + DND    L  A+ + ++E+   L N
Sbjct:  567 NGSKQEENSETTKLLLEHNADCKIEDANGRVALHLAAYHGDPCLQLILEKHPIVDVMDNMGQTPLMLAASQGQLEAVQFLTKTAKADVDAIDNNGRTALQWAAINGHPEVVEFLCSVGSDEGHKDNDGAVALHYAVTHDHVELVKHLTN 715

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BLAST of SMED30005308 vs. Ensembl Fly
Match: Sans (gene:FBgn0033785 transcript:FBtr0087791)

HSP 1 Score: 78.1814 bits (191), Expect = 8.685e-16
Identity = 46/145 (31.72%), Postives = 72/145 (49.66%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEK 475
            A K    ++L+ A+  +  + D + M  + WAA+ G L+ L+L+  RG    K D  G T LH A    H+  V +LV +G+ I+ LD D +S   LA +N   EI  +LD         + K  + ++   +K   KRV+ Y K
Sbjct:    9 AAKDGLLDVLAAATRKDTNAKDSDSMTPVMWAAFEGRLDALRLLCGRGGDPDKCDQFGNTALHLASAKGHLHCVDFLVKFGVNIYALDIDKHSAKDLAAINGRDEILRYLDVAFTNFEATEKKKSKALKELAEKNCEKRVREYMK 153

[Close]

BLAST of SMED30005308 vs. Ensembl Fly
Match: Sans (gene:FBgn0033785 transcript:FBtr0087790)

HSP 1 Score: 78.1814 bits (191), Expect = 8.685e-16
Identity = 46/145 (31.72%), Postives = 72/145 (49.66%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEK 475
            A K    ++L+ A+  +  + D + M  + WAA+ G L+ L+L+  RG    K D  G T LH A    H+  V +LV +G+ I+ LD D +S   LA +N   EI  +LD         + K  + ++   +K   KRV+ Y K
Sbjct:    9 AAKDGLLDVLAAATRKDTNAKDSDSMTPVMWAAFEGRLDALRLLCGRGGDPDKCDQFGNTALHLASAKGHLHCVDFLVKFGVNIYALDIDKHSAKDLAAINGRDEILRYLDVAFTNFEATEKKKSKALKELAEKNCEKRVREYMK 153

[Close]

BLAST of SMED30005308 vs. Ensembl Fly
Match: Mbs (gene:FBgn0005536 transcript:FBtr0308212)

HSP 1 Score: 58.9214 bits (141), Expect = 2.532e-9
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 2

Query:   32 CINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQ 385
            C++  K    +LL   +     +VD  G+ ++H A    +L++++ ++ RG  I + DN G T LH       V I RYLV  G  +  +++D +  L LAI  Q++ +  +++   Q
Sbjct:   54 CLSGDKDEVVQLLDQGADINTANVD--GLTALHQACIDDNLDMVEFLVERGADINRQDNEGWTPLHATASCGFVSIARYLVENGADVAAVNSDGDLALDLAIDVQHMAMIDYMEKMVQ 169

[Close]

BLAST of SMED30005308 vs. Ensembl Fly
Match: Mbs (gene:FBgn0005536 transcript:FBtr0308215)

HSP 1 Score: 58.9214 bits (141), Expect = 2.640e-9
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 2

Query:   32 CINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQ 385
            C++  K    +LL   +     +VD  G+ ++H A    +L++++ ++ RG  I + DN G T LH       V I RYLV  G  +  +++D +  L LAI  Q++ +  +++   Q
Sbjct:   54 CLSGDKDEVVQLLDQGADINTANVD--GLTALHQACIDDNLDMVEFLVERGADINRQDNEGWTPLHATASCGFVSIARYLVENGADVAAVNSDGDLALDLAIDVQHMAMIDYMEKMVQ 169

[Close]

BLAST of SMED30005308 vs. Ensembl Fly
Match: Mbs (gene:FBgn0005536 transcript:FBtr0301472)

HSP 1 Score: 58.9214 bits (141), Expect = 2.835e-9
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 2

Query:   32 CINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQ 385
            C++  K    +LL   +     +VD  G+ ++H A    +L++++ ++ RG  I + DN G T LH       V I RYLV  G  +  +++D +  L LAI  Q++ +  +++   Q
Sbjct:   54 CLSGDKDEVVQLLDQGADINTANVD--GLTALHQACIDDNLDMVEFLVERGADINRQDNEGWTPLHATASCGFVSIARYLVENGADVAAVNSDGDLALDLAIDVQHMAMIDYMEKMVQ 169

[Close]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Match: anks4b (ankyrin repeat and sterile alpha motif domain containing 4B [Source:ZFIN;Acc:ZDB-GENE-040704-29])

HSP 1 Score: 110.923 bits (276), Expect = 3.042e-27
Identity = 54/140 (38.57%), Postives = 84/140 (60.00%), Query Frame = 2

Query:   62 ELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +LL  A+  +  + DE+GM    WAAY G +E L+LV SRG    K D  G T LHHA    H++I+ +LVN+G  ++ LDNDF++P+ +A    +++   FLDN A ++   + K V R++ +  K A +RVK  E+ +
Sbjct:   14 DLLKEATRKDLNTPDEDGMTPTLWAAYHGHIEALQLVCSRGGDPNKSDIWGNTPLHHAAANGHMQILSFLVNFGANLFALDNDFHTPMDVAASRDHMDCVRFLDNSAAQQTGQNAKRVARLKEQATKDAERRVKQCERVK 153

[Close]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Match: ush1ga (Usher syndrome 1Ga (autosomal recessive) [Source:ZFIN;Acc:ZDB-GENE-131120-173])

HSP 1 Score: 98.5969 bits (244), Expect = 1.064e-22
Identity = 46/141 (32.62%), Postives = 82/141 (58.16%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            A +    +LL  A+  +  + DE+GM    WAAY G+L+ L+L+  RG +  K D  G T LH A    H+  + +LV++G  +W LDND+++PL +A    +++   +LD+ A ++   +PK V +++ +  + A +R+K
Sbjct:    9 AARDGYLDLLKEATRKDLNAPDEDGMTPTLWAAYHGNLDALRLIAGRGGNPDKCDIWGNTPLHLAASNGHLNCLSFLVSFGANVWCLDNDYHTPLDMAATKNHMDCVRYLDSIAAKQTALNPKLVSKLKDRAFRDAERRIK 149

[Close]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Match: ush1gb (Usher syndrome 1Gb (autosomal recessive) [Source:ZFIN;Acc:ZDB-GENE-110411-55])

HSP 1 Score: 95.9005 bits (237), Expect = 4.637e-22
Identity = 46/146 (31.51%), Postives = 83/146 (56.85%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKF 478
            A +  +  LL  A+     + DE+GM    WAA  G+++ L+L++ RG    K D  G T LH A    H+  + +LV++G  +W LDND+++PL +A    +++   +LD+ A +++  +PK V +++ +  + A KR++  EK 
Sbjct:    8 AARDGHLLLLKEATRKHLNAPDEDGMTPTLWAAQHGNVDALRLIVGRGGDPDKCDIWGNTPLHLAAANGHLSCLSFLVSFGANVWCLDNDYHTPLDMAASRSHMDCVRYLDSIAAKQITINPKLVSKLKERGFRNAEKRIRNCEKL 153

[Close]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Match: ush1gb (Usher syndrome 1Gb (autosomal recessive) [Source:ZFIN;Acc:ZDB-GENE-110411-55])

HSP 1 Score: 96.6709 bits (239), Expect = 5.889e-22
Identity = 46/147 (31.29%), Postives = 84/147 (57.14%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +  +  LL  A+     + DE+GM    WAA  G+++ L+L++ RG    K D  G T LH A    H+  + +LV++G  +W LDND+++PL +A    +++   +LD+ A +++  +PK V +++ +  + A KR++  EK +
Sbjct:    8 AARDGHLLLLKEATRKHLNAPDEDGMTPTLWAAQHGNVDALRLIVGRGGDPDKCDIWGNTPLHLAAANGHLSCLSFLVSFGANVWCLDNDYHTPLDMAASRSHMDCVRYLDSIAAKQITINPKLVSKLKERGFRNAEKRIRNCEKLQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Match: ush1ga (Usher syndrome 1Ga (autosomal recessive) [Source:ZFIN;Acc:ZDB-GENE-131120-173])

HSP 1 Score: 62.7734 bits (151), Expect = 1.248e-10
Identity = 28/89 (31.46%), Postives = 53/89 (59.55%), Query Frame = 2

Query:  197 KIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            K D  G T LH A    H+  + +LV++G  +W LDND+++PL +A    +++   +LD+ A ++   +PK V +++ +  + A +R+K
Sbjct:   20 KCDIWGNTPLHLAASNGHLNCLSFLVSFGANVWCLDNDYHTPLDMAATKNHMDCVRYLDSIAAKQTALNPKLVSKLKDRAFRDAERRIK 108

[Close]

BLAST of SMED30005308 vs. Ensembl Xenopus
Match: med6 (mediator complex subunit 6 [Source:Xenbase;Acc:XB-GENE-967312])

HSP 1 Score: 101.293 bits (251), Expect = 1.617e-23
Identity = 50/147 (34.01%), Postives = 84/147 (57.14%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    +LL  A+  +  S DE+GM    WAAY G L  L++++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A    ++E   +LD+ A ++   +PK V +++ +  ++A KR++   K +
Sbjct:    8 AARDGFLDLLKEATRRDLNSPDEDGMTPTLWAAYHGHLGALRVIVSRGGDPDKCDIWGNTPLHVAAANGHLNCLSFLVSFGANIWCLDNDYHTPLDMAASKGHMECVRYLDSIAAKQTGLNPKLVSKLKERAFREAEKRIRDCAKLQ 154

[Close]

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    +LL  A+  +  S DE+GM    WAAY G L  L++++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A    ++E   +LD+ A ++   +PK V +++ +  ++A KR++   K +
Sbjct:    8 AARDGFLDLLKEATRRDLNSPDEDGMTPTLWAAYHGHLGALRVIVSRGGDPDKCDIWGNTPLHVAAANGHLNCLSFLVSFGANIWCLDNDYHTPLDMAASKGHMECVRYLDSIAAKQTGLNPKLVSKLKERAFREAEKRIRDCAKLQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Xenopus
Match: anks4b (ankyrin repeat and sterile alpha motif domain containing 4B [Source:NCBI gene;Acc:100158613])

HSP 1 Score: 95.9005 bits (237), Expect = 9.356e-22
Identity = 53/154 (34.42%), Postives = 81/154 (52.60%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A      E+L  A+  +  + D +GM     AAY G L++L+++  RG    K D  G T LHHA    H   V +LVN+G  I+ LDN + +PL +A +    E    LD  A ++ + +PK VER++ +  K A ++VK  EK +
Sbjct:    1 MSTRYHQAAIDGYQEILKEATKKDVNTTDGDGMTPTILAAYHGHLDVLEILCHRGGDPDKSDIWGNTPLHHAAEKGHASCVSFLVNFGANIFALDNKYRTPLDVAAIKDRTECVKILDGAANKQASLNPKKVERLKEQAVKDADRKVKECEKIQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Xenopus
Match: med6 (mediator complex subunit 6 [Source:Xenbase;Acc:XB-GENE-967312])

HSP 1 Score: 66.2402 bits (160), Expect = 1.215e-11
Identity = 31/95 (32.63%), Postives = 56/95 (58.95%), Query Frame = 2

Query:  197 KIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A    ++E   +LD+ A ++   +PK V +++ +  ++A KR++   K +
Sbjct:   26 KCDIWGNTPLHVAAANGHLNCLSFLVSFGANIWCLDNDYHTPLDMAASKGHMECVRYLDSIAAKQTGLNPKLVSKLKERAFREAEKRIRDCAKLQ 120

[Close]

BLAST of SMED30005308 vs. Ensembl Xenopus
Match: grsf1 (G-rich RNA sequence binding factor 1 [Source:Xenbase;Acc:XB-GENE-965150])

HSP 1 Score: 60.8474 bits (146), Expect = 1.306e-10
Identity = 26/66 (39.39%), Postives = 40/66 (60.61%), Query Frame = 2

Query:   83 SSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNY 280
             ++C +    G  ++H AAY G LE+++L+L RG    K D++GRT LH A  G H +I   L+N+
Sbjct:   90 GADCNAQTHGGCTALHRAAYCGHLEVVQLLLERGADPAKTDSDGRTALHKAAEGGHQKICHLLLNH 155

[Close]

BLAST of SMED30005308 vs. Ensembl Mouse
Match: Ush1g (USH1 protein network component sans [Source:MGI Symbol;Acc:MGI:2450757])

HSP 1 Score: 107.071 bits (266), Expect = 1.137e-25
Identity = 53/147 (36.05%), Postives = 88/147 (59.86%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    ELL  A+  E  + DE+GM    WAAY G+LE L+L++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A +  ++E   +LD+ A ++ + +PK V +++ K  ++A +R++   K +
Sbjct:    8 AARDGYLELLKEATRKELNAPDEDGMTPTLWAAYHGNLESLRLIVSRGGDPDKCDIWGNTPLHLAASNGHLHCLSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKMQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Mouse
Match: Anks4b (ankyrin repeat and sterile alpha motif domain containing 4B [Source:MGI Symbol;Acc:MGI:1919324])

HSP 1 Score: 95.5153 bits (236), Expect = 9.175e-22
Identity = 56/154 (36.36%), Postives = 80/154 (51.95%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A   S  ELL  A+  +    DE+GM     AAY G+LE L+++ SRG    K D  G T LH+A    H   + +LVN+G  I+ LDND  SPL  A   +  E  + LD  A  +   +PK V R++ +  K A K++K  E+ +
Sbjct:    1 MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDKCDIWGNTPLHYAASNGHTHCISFLVNFGANIFALDNDLKSPLDAAASREQKECVALLDKAATVQNTMNPKRVTRLKEQALKNARKQMKECERLQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Mouse
Match: Ehmt2 (euchromatic histone lysine N-methyltransferase 2 [Source:MGI Symbol;Acc:MGI:2148922])

HSP 1 Score: 64.3142 bits (155), Expect = 3.956e-11
Identity = 33/91 (36.26%), Postives = 53/91 (58.24%), Query Frame = 2

Query:  125 IHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +H AA  G +EI  ++L  G +I  +D   RT L  AV+ NH+E+ RY+V  G C+++ + D ++ L  A    N+E+ S L +  Q +VN
Sbjct:  263 LHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVN 353

[Close]

BLAST of SMED30005308 vs. Ensembl Mouse
Match: Ehmt2 (euchromatic histone lysine N-methyltransferase 2 [Source:MGI Symbol;Acc:MGI:2148922])

HSP 1 Score: 64.6994 bits (156), Expect = 4.417e-11
Identity = 34/101 (33.66%), Postives = 57/101 (56.44%), Query Frame = 2

Query:   95 ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +S  ++    +H AA  G +EI  ++L  G +I  +D   RT L  AV+ NH+E+ RY+V  G C+++ + D ++ L  A    N+E+ S L +  Q +VN
Sbjct:  698 QSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVN 798

[Close]

BLAST of SMED30005308 vs. Ensembl Mouse
Match: Ehmt2 (euchromatic histone lysine N-methyltransferase 2 [Source:MGI Symbol;Acc:MGI:2148922])

HSP 1 Score: 64.6994 bits (156), Expect = 4.439e-11
Identity = 34/101 (33.66%), Postives = 57/101 (56.44%), Query Frame = 2

Query:   95 ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +S  ++    +H AA  G +EI  ++L  G +I  +D   RT L  AV+ NH+E+ RY+V  G C+++ + D ++ L  A    N+E+ S L +  Q +VN
Sbjct:  732 QSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVN 832

[Close]

BLAST of SMED30005308 vs. UniProt/SwissProt
Match: sp|Q495M9|USH1G_HUMAN (Usher syndrome type-1G protein OS=Homo sapiens OX=9606 GN=USH1G PE=1 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.365e-25
Identity = 53/147 (36.05%), Postives = 88/147 (59.86%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    ELL  A+  E  + DE+GM    WAAY G+LE L+L++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A +  ++E   +LD+ A ++ + +PK V +++ K  ++A +R++   K +
Sbjct:    8 AARDGYLELLKEATRKELNAPDEDGMTPTLWAAYHGNLESLRLIVSRGGDPDKCDIWGNTPLHLAASNGHLHCLSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKLQ 154

[Close]

BLAST of SMED30005308 vs. UniProt/SwissProt
Match: sp|Q80T11|USH1G_MOUSE (Usher syndrome type-1G protein homolog OS=Mus musculus OX=10090 GN=Ush1g PE=1 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.950e-25
Identity = 53/147 (36.05%), Postives = 88/147 (59.86%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +    ELL  A+  E  + DE+GM    WAAY G+LE L+L++SRG    K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A +  ++E   +LD+ A ++ + +PK V +++ K  ++A +R++   K +
Sbjct:    8 AARDGYLELLKEATRKELNAPDEDGMTPTLWAAYHGNLESLRLIVSRGGDPDKCDIWGNTPLHLAASNGHLHCLSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKMQ 154

[Close]

BLAST of SMED30005308 vs. UniProt/SwissProt
Match: sp|Q8K3X6|ANS4B_MOUSE (Ankyrin repeat and SAM domain-containing protein 4B OS=Mus musculus OX=10090 GN=Anks4b PE=1 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 6.418e-21
Identity = 56/154 (36.36%), Postives = 80/154 (51.95%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A   S  ELL  A+  +    DE+GM     AAY G+LE L+++ SRG    K D  G T LH+A    H   + +LVN+G  I+ LDND  SPL  A   +  E  + LD  A  +   +PK V R++ +  K A K++K  E+ +
Sbjct:    1 MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDKCDIWGNTPLHYAASNGHTHCISFLVNFGANIFALDNDLKSPLDAAASREQKECVALLDKAATVQNTMNPKRVTRLKEQALKNARKQMKECERLQ 154

[Close]

BLAST of SMED30005308 vs. UniProt/SwissProt
Match: sp|Q8N8V4|ANS4B_HUMAN (Ankyrin repeat and SAM domain-containing protein 4B OS=Homo sapiens OX=9606 GN=ANKS4B PE=1 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 8.385e-21
Identity = 55/154 (35.71%), Postives = 80/154 (51.95%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +ST    A   S  ELL  A+  +    DE+GM     AAY G+LE L+++ SRG    + D  G T LH A    H   V +LVN+G  I+ LDND  +PL  A   +  E  + LD  A  +   +PK V R++ + QK A +++K  E+ +
Sbjct:    1 MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDRCDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQ 154

[Close]

BLAST of SMED30005308 vs. UniProt/SwissProt
Match: sp|Q5ZLC8|ANR52_CHICK (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C OS=Gallus gallus OX=9031 GN=ANKRD52 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.562e-10
Identity = 37/104 (35.58%), Postives = 60/104 (57.69%), Query Frame = 2

Query:   38 NAVKQSNTELLSM-----ASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLA 334
            +AV   + E++++     AS S C+  D      IHWAA+LG LE+LKL+++RG  +   D  G T+LH A     +E+VR+L+  G+ I   ++  N+ L +A
Sbjct:  146 HAVHSGHLEMVNLLLNKGASLSTCDKKDRQ---PIHWAAFLGHLEVLKLLVARGADVMCKDKKGYTLLHTAAASGQIEVVRHLLRLGVEIDEPNSFGNTALHIA 246

[Close]

BLAST of SMED30005308 vs. TrEMBL
Match: A0A1S3HTP5 (Usher syndrome type-1G protein OS=Lingula unguis OX=7574 GN=LOC106157346 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 5.663e-27
Identity = 57/155 (36.77%), Postives = 90/155 (58.06%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRI 484
            ++T    A +    +LL  A+  + ++ DE+GM    WAAY G+L+ L+L++ RG    K D  G T LHHA    H+  V YLVN+G+ IW LDND +S L LA +  ++++  FLD    ++   +PK V++++ K   +A +R K YEK + 
Sbjct:    1 MATKFHQAARDGYLDLLREATRKDADTPDEDGMTPTLWAAYFGNLDALRLLVGRGGDADKCDLLGNTALHHASFNGHLHCVTYLVNFGVNIWALDNDHHSSLDLASMKNHMDVVRFLDGVVAKQSATNPKFVKKMKEKAVLEADERFKRYEKLQF 155

[Close]

BLAST of SMED30005308 vs. TrEMBL
Match: A0A1S3KI95 (Usher syndrome type-1G protein-like OS=Lingula unguis OX=7574 GN=LOC106182100 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.159e-26
Identity = 57/155 (36.77%), Postives = 90/155 (58.06%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRI 484
            ++T    A +    +LL  A+  + ++ DE+GM    WAAY G+L+ L+L++ RG    K D  G T LHHA    H+  V YLVN+G+ IW LDND +S L LA +  ++++  FLD    ++   +PK V++++ K   +A +R K YEK + 
Sbjct:    1 MATKFHQAARDGYLDLLREATRKDADTPDEDGMTPTLWAAYFGNLDALRLLVGRGGDADKCDLLGNTSLHHASFNGHLHCVTYLVNFGVNIWALDNDHHSSLDLASMKNHMDVVRFLDGVVAKQSATNPKFVKKMKEKAVLEADERFKRYEKLQF 155

[Close]

BLAST of SMED30005308 vs. TrEMBL
Match: A0A267DG34 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022693g3 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.239e-26
Identity = 56/146 (38.36%), Postives = 88/146 (60.27%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKF 478
            A K +  +LL  A+ SEC++VD+NGM  + WAA+ G LE L++++ RG    + D+ G T LH A +   +  V YLVN+G  IW LD D +S L LA +N++ +I  FLD+ A R+   + + V +++ K   +A +R K  +K 
Sbjct:   11 AAKDNYLDLLREATRSECDAVDDNGMTPVMWAAFYGHLEALRVLMGRGADPDRADHMGSTALHFAALNGRMNCVTYLVNFGANIWTLDVDLHSALDLAALNEHRDIVDFLDDVAGRQAEVNKRTVAKMREKAVLEAQRRAKKRDKL 156

[Close]

BLAST of SMED30005308 vs. TrEMBL
Match: A0A1I8JLV4 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022693g1 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.277e-26
Identity = 56/146 (38.36%), Postives = 88/146 (60.27%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKF 478
            A K +  +LL  A+ SEC++VD+NGM  + WAA+ G LE L++++ RG    + D+ G T LH A +   +  V YLVN+G  IW LD D +S L LA +N++ +I  FLD+ A R+   + + V +++ K   +A +R K  +K 
Sbjct:   11 AAKDNYLDLLREATRSECDAVDDNGMTPVMWAAFYGHLEALRVLMGRGADPDRADHMGSTALHFAALNGRMNCVTYLVNFGANIWTLDVDLHSALDLAALNEHRDIVDFLDDVAGRQAEVNKRTVAKMREKAVLEAQRRAKKRDKL 156

[Close]

BLAST of SMED30005308 vs. TrEMBL
Match: A0A267GCM3 (ANK_REP_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022693g2 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 4.376e-26
Identity = 56/146 (38.36%), Postives = 88/146 (60.27%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKF 478
            A K +  +LL  A+ SEC++VD+NGM  + WAA+ G LE L++++ RG    + D+ G T LH A +   +  V YLVN+G  IW LD D +S L LA +N++ +I  FLD+ A R+   + + V +++ K   +A +R K  +K 
Sbjct:   11 AAKDNYLDLLREATRSECDAVDDNGMTPVMWAAFYGHLEALRVLMGRGADPDRADHMGSTALHFAALNGRMNCVTYLVNFGANIWTLDVDLHSALDLAALNEHRDIVDFLDDVAGRQAEVNKRTVAKMREKAVLEAQRRAKKRDKL 156

[Close]

BLAST of SMED30005308 vs. Ensembl Cavefish
Match: ush1gb (Usher syndrome type-1G protein homolog [Source:NCBI gene;Acc:103036832])

HSP 1 Score: 103.99 bits (258), Expect = 1.087e-24
Identity = 48/141 (34.04%), Postives = 83/141 (58.87%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            A +    +LL  A+  +  S DE+GM    WAA+ G L+ L+LVL RG    + D  G T LH A    H+  + YLV++G  +W LDND+++PL +A +  +++   +LD+ A +++  +PK V +++ +  + A +R+K
Sbjct:    8 AARDGRLDLLRQATRRDLNSPDEDGMTPTLWAAHHGHLDSLRLVLGRGGDPDRCDIWGNTPLHLAAANGHLNCLTYLVSFGANVWCLDNDYHTPLDMAAMRSHMDCVRYLDSIAAKQITLNPKLVSKLKDRAFRNAERRIK 148

[Close]

BLAST of SMED30005308 vs. Ensembl Cavefish
Match: anks4b (ankyrin repeat and sterile alpha motif domain containing 4B [Source:NCBI gene;Acc:111193243])

HSP 1 Score: 99.3673 bits (246), Expect = 3.743e-23
Identity = 54/154 (35.06%), Postives = 85/154 (55.19%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            +S Y  +A+     +LL  A+  +  + DE+GM    WAAY G LE L+L+ SRG      D  G T LHHA    H+ ++ +LVN+G  ++ LDNDF++P+ +A     +    FLD+ A  + + + K V +++ +  K A  RVK  EK +
Sbjct:    1 MSRYHKSAI-DGYLDLLKEATKKDLNTRDEDGMTPTLWAAYHGRLEALQLICSRGGDPNLSDIWGNTPLHHAAANGHMHVLSFLVNFGANLFALDNDFHTPMDVAASRDQMNCVRFLDSAASNQTSQNAKRVAQLKKQAVKDAEVRVKQCEKVK 153

[Close]

BLAST of SMED30005308 vs. Ensembl Cavefish
Match: ush1ga (USH1 protein network component sans [Source:NCBI gene;Acc:111192967])

HSP 1 Score: 97.4413 bits (241), Expect = 2.435e-22
Identity = 45/141 (31.91%), Postives = 83/141 (58.87%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            A +    +LL  A+  +  + DE+GM    WAAY G+L+ L+L+++RG +  K D  G T LH +    H+  + +LV++G   W LDND+++PL +A    +++   +LD+ A ++   +PK V +++ +  + A +R+K
Sbjct:    8 AARDGYLDLLKEATRKDLNAPDEDGMTPTLWAAYHGNLDALRLIVARGGNPDKCDIWGNTPLHLSAANGHLNCLSFLVSFGANTWCLDNDYHTPLDMAATKNHMDCVRYLDSIAAKQTALNPKLVAKLKDRAFRDAERRIK 148

[Close]

BLAST of SMED30005308 vs. Ensembl Cavefish
Match: ush1gb (Usher syndrome type-1G protein homolog [Source:NCBI gene;Acc:103036832])

HSP 1 Score: 67.0106 bits (162), Expect = 4.649e-12
Identity = 27/84 (32.14%), Postives = 53/84 (63.10%), Query Frame = 2

Query:  212 GRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            G T LH A    H+  + YLV++G  +W LDND+++PL +A +  +++   +LD+ A +++  +PK V +++ +  + A +R+K
Sbjct:   44 GNTPLHLAAANGHLNCLTYLVSFGANVWCLDNDYHTPLDMAAMRSHMDCVRYLDSIAAKQITLNPKLVSKLKDRAFRNAERRIK 127

[Close]

BLAST of SMED30005308 vs. Ensembl Cavefish
Match: ANKRD52 (ankyrin repeat domain 52 [Source:HGNC Symbol;Acc:HGNC:26614])

HSP 1 Score: 65.0846 bits (157), Expect = 2.525e-11
Identity = 37/115 (32.17%), Postives = 63/115 (54.78%), Query Frame = 2

Query:   38 NAVKQSNTELLSMA--SSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDN 376
            +A    +TE++++     +   + D+    +IHWAAYLG LEI+K+++SR   +   D  G T LH A I  H+++V+YL+  G  I   +   N+ L +A  N    +++ L N
Sbjct:  134 HAAYSGHTEMVTLLLNKGANLSASDKKDRQAIHWAAYLGHLEIVKVLVSRNADVACRDKRGYTPLHVAAINGHIDVVKYLLRLGAEIDEPNAFGNTSLHMACYNGQEAVANELVN 248

HSP 2 Score: 57.7658 bits (138), Expect = 6.489e-9
Identity = 30/95 (31.58%), Postives = 51/95 (53.68%), Query Frame = 2

Query:   86 SECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            S     D NG  ++H AAY G  E++ L+L++G ++   D   R  +H A    H+EIV+ LV+    +   D    +PL +A +N +I++  +L
Sbjct:  119 SSLNVADRNGRTALHHAAYSGHTEMVTLLLNKGANLSASDKKDRQAIHWAAYLGHLEIVKVLVSRNADVACRDKRGYTPLHVAAINGHIDVVKYL 213

[Close]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.9214 bits (141), Expect = 4.792e-10
Identity = 38/133 (28.57%), Postives = 66/133 (49.62%), Query Frame = 2

Query:    5 TFKPKISTYCINAVKQSNTELL--SMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSF-LDNCAQREV 394
            +F+   +T  + A +  N E L   +    +  + ++NG+N++H A+  G ++++  +L R   I      G T LH A +    +IVR LV +G  I     +  +PL +A    ++EI  F LDN A + V
Sbjct:    6 SFQSDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSV 138

HSP 2 Score: 46.9802 bits (110), Expect = 3.676e-6
Identity = 34/117 (29.06%), Postives = 56/117 (47.86%), Query Frame = 2

Query:   62 ELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKA 412
            ELL +   +   +  E+G+  IH A+++G L +++L+L  G S    +  G T LH A     +E+V+ L+  G  +     +  +PL +A      EI   L    QR   +DP A
Sbjct:  387 ELL-LKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLL---LQR--GSDPNA 497

HSP 3 Score: 45.8246 bits (107), Expect = 7.736e-6
Identity = 30/104 (28.85%), Postives = 55/104 (52.88%), Query Frame = 2

Query:   59 TELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            TELL     +E ++  + G  ++H AA  G  +I+++++  G +I     NG T L+ A   NH+EIV++L++ G        D  +PL +A+   + ++   L
Sbjct:   61 TELLK--REAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLL 162

[Close]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 1.560e-9
Identity = 38/131 (29.01%), Postives = 64/131 (48.85%), Query Frame = 2

Query:   11 KPKISTYCINAVKQSNTELL--SMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSF-LDNCAQREV 394
            K   +T  + A +  N E L   +    +  + ++NG+N++H A+  G ++++  +L R   I      G T LH A +    +IVR LV +G  I     +  +PL +A    ++EI  F LDN A + V
Sbjct:    7 KSDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSV 137

HSP 2 Score: 47.3654 bits (111), Expect = 3.014e-6
Identity = 34/117 (29.06%), Postives = 56/117 (47.86%), Query Frame = 2

Query:   62 ELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKA 412
            ELL +   +   +  E+G+  IH A+++G L +++L+L  G S    +  G T LH A     +E+V+ L+  G  +     +  +PL +A      EI   L    QR   +DP A
Sbjct:  394 ELL-LKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLL---LQR--GSDPNA 504

HSP 3 Score: 46.2098 bits (108), Expect = 6.231e-6
Identity = 30/104 (28.85%), Postives = 55/104 (52.88%), Query Frame = 2

Query:   59 TELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            TELL     +E ++  + G  ++H AA  G  +I+++++  G +I     NG T L+ A   NH+EIV++L++ G        D  +PL +A+   + ++   L
Sbjct:   60 TELLK--REAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLL 161

[Close]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.8398 bits (133), Expect = 5.631e-9
Identity = 27/101 (26.73%), Postives = 58/101 (57.43%), Query Frame = 2

Query:   74 MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVN-QNIEISSFLD 373
            +A  +   + D+    +IHWAAY+G  E++K++++ G  ++  D  G + LH A     + +V+ L++ G+ I + ++  N+ L +A  N Q++ ++  +D
Sbjct:  158 VAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELID 258

HSP 2 Score: 49.2914 bits (116), Expect = 7.431e-7
Identity = 30/102 (29.41%), Postives = 49/102 (48.04%), Query Frame = 2

Query:   65 LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            ++ M SSS     D  G  ++H AA+ G  E++  ++++G +I   D   R  +H A    H E+V+ LV +G  +   D    SPL  A  +  I +   L
Sbjct:  124 IIPMLSSSNVS--DRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLL 223

HSP 3 Score: 49.2914 bits (116), Expect = 7.978e-7
Identity = 26/89 (29.21%), Postives = 46/89 (51.69%), Query Frame = 2

Query:   89 ECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIE 355
            E  +VD+     +H AAYLG+ EI++L++  G  +   DN   T LH A        V+ L+ +   +   D ++ +PL +A  N+ ++
Sbjct:   31 EVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVK 119

HSP 4 Score: 48.1358 bits (113), Expect = 1.459e-6
Identity = 39/129 (30.23%), Postives = 60/129 (46.51%), Query Frame = 2

Query:   53 SNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAI------------VNQNIEISSFLD---NCAQREV 394
            SN  +LS  +  E ++ DE G   +H AA  G++E L L+LS G  + K D   RT LH+A    +V+ V  LV  G  +   D    +PL  A             V   I + S++D    C++ ++
Sbjct:  403 SNEHVLS--AGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQI 529

[Close]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010339.1 (pep scaffold:Pmarinus_7.0:GL476759:135524:215537:1 gene:ENSPMAG00000009360.1 transcript:ENSPMAT00000010339.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 7.109e-9
Identity = 33/98 (33.67%), Postives = 52/98 (53.06%), Query Frame = 2

Query:   65 LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEI 358
            LLS  +S  C  VD+ G++++ WA   G   +++ ++ RG +I   D NGRT L  A       +V+YLV +G  I ++D     PL  AI  +N  +
Sbjct: 1063 LLSKGASIGC--VDKEGLSALSWACLKGHQPVVQSLVGRGAAIDHTDKNGRTPLDLAAFYGDAGVVQYLVEHGAMIEHVDYSGMRPLDRAIGCRNTAV 1158

HSP 2 Score: 49.6766 bits (117), Expect = 5.006e-7
Identity = 24/70 (34.29%), Postives = 39/70 (55.71%), Query Frame = 2

Query:   68 LSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVN 277
            L + S ++ ++  E+GM  + +AA  G LEI+  +  R   +  +D NG+  L HA +  H  IV +LVN
Sbjct:  847 LLLDSGADMDTASESGMTPLCYAAAAGQLEIVARLCRRSAKLAHVDRNGQCALVHAALRGHRSIVEFLVN 916

[Close]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010338.1 (pep scaffold:Pmarinus_7.0:GL476759:135524:215537:1 gene:ENSPMAG00000009360.1 transcript:ENSPMAT00000010338.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 7.114e-9
Identity = 33/98 (33.67%), Postives = 52/98 (53.06%), Query Frame = 2

Query:   65 LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEI 358
            LLS  +S  C  VD+ G++++ WA   G   +++ ++ RG +I   D NGRT L  A       +V+YLV +G  I ++D     PL  AI  +N  +
Sbjct: 1071 LLSKGASIGC--VDKEGLSALSWACLKGHQPVVQSLVGRGAAIDHTDKNGRTPLDLAAFYGDAGVVQYLVEHGAMIEHVDYSGMRPLDRAIGCRNTAV 1166

HSP 2 Score: 49.6766 bits (117), Expect = 5.010e-7
Identity = 24/70 (34.29%), Postives = 39/70 (55.71%), Query Frame = 2

Query:   68 LSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVN 277
            L + S ++ ++  E+GM  + +AA  G LEI+  +  R   +  +D NG+  L HA +  H  IV +LVN
Sbjct:  855 LLLDSGADMDTASESGMTPLCYAAAAGQLEIVARLCRRSAKLAHVDRNGQCALVHAALRGHRSIVEFLVN 924

[Close]

BLAST of SMED30005308 vs. Ensembl Nematostella
Match: EDO35466 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLB8])

HSP 1 Score: 72.0182 bits (175), Expect = 3.148e-14
Identity = 43/162 (26.54%), Postives = 81/162 (50.00%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLV---------------LSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +  N  +L  A+  +    DE+ M  +H+AA  G++E L+L+               LSRG    K + +G T +H A     +  + +L N+G  IW LDN+ ++PL+ A ++  ++    LD     ++  + K  ER++ + + +AI+R++   K +
Sbjct:    8 AARDGNLNILKNATKRDTNRRDEDYMTPVHYAASCGNVEALRLLSGQNDDYKLISLSSFLSRG-DPDKTNCDGSTAVHLAAGCGQLNCLSFLTNFGANIWALDNEGHTPLEEAAIHGRMDCVRHLDGLVAIQMLRNKKETERLKRRAKSEAIRRMRKQSKMQ 168

[Close]

BLAST of SMED30005308 vs. Ensembl Nematostella
Match: EDO39546 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9P8])

HSP 1 Score: 54.299 bits (129), Expect = 1.032e-9
Identity = 30/98 (30.61%), Postives = 57/98 (58.16%), Query Frame = 2

Query:  104 DENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAI-VNQNIEISSFLDNCAQREV 394
            DE G   +H AA  G++E+L+L+   G  +  +D  G T++H   + NHV+++++LV++G+     + + ++PL LA+      E+  FL    Q+E+
Sbjct:    6 DERGRTPMHMAAMRGNVEMLELLYQHGGKLGTVDLIGNTLMH---MCNHVDVLQFLVDHGLSATERNINGDTPLVLAMRFAAKSEVCRFLKELEQKEM 100

[Close]

BLAST of SMED30005308 vs. Ensembl Nematostella
Match: EDO35987 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJZ9])

HSP 1 Score: 51.9878 bits (123), Expect = 9.267e-9
Identity = 28/87 (32.18%), Postives = 50/87 (57.47%), Query Frame = 2

Query:   47 KQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDN 307
            K SN E     ++++ ++ D  G  ++H+AA  G +E++KL+++RG  I   D+ G+T L  A I +  E V  L+ +G C+   D+
Sbjct:   21 KGSNLE-----ATTDMDAHDMFGRTALHYAAQQGHMEMIKLLVTRGADIHTRDSEGQTPLFQATIFDQKEAVNVLLLHGACVNAFDH 102

[Close]

BLAST of SMED30005308 vs. Ensembl Nematostella
Match: EDO49564 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGN2])

HSP 1 Score: 52.7582 bits (125), Expect = 2.799e-8
Identity = 33/80 (41.25%), Postives = 49/80 (61.25%), Query Frame = 2

Query:  134 AAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNL-DNDFNSPLKLAIVNQNIEISSFL 370
            AA  G+LE +K V+         +++G T LH++V GNH+ IV++LV YG C  NL D+   +PL  A VN N+++  FL
Sbjct:   28 AALEGELEHVKQVIKNVEDPSTANSDGITALHNSVCGNHLSIVQFLVQYG-CDVNLPDSHGWTPLHCAAVNNNLKVVQFL 106

HSP 2 Score: 46.9802 bits (110), Expect = 2.377e-6
Identity = 20/70 (28.57%), Postives = 43/70 (61.43%), Query Frame = 2

Query:   98 SVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDN 307
            + + +G+ ++H +     L I++ ++  GC +   D++G T LH A + N++++V++LV +G CI+   N
Sbjct:   49 TANSDGITALHNSVCGNHLSIVQFLVQYGCDVNLPDSHGWTPLHCAAVNNNLKVVQFLVEHGACIFATTN 118

[Close]

BLAST of SMED30005308 vs. Ensembl Nematostella
Match: EDO49269 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH05])

HSP 1 Score: 53.9138 bits (128), Expect = 4.165e-8
Identity = 36/127 (28.35%), Postives = 62/127 (48.82%), Query Frame = 2

Query:  125 IHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVN-QNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRV---KAYEKFRITEF 493
            I+ A   G  +I++ +L RG  +       RT LH A    +  I + L+ +G  +   D+ F +PL L++   QN+E+S  L     R + N+ + V  V ++ Q K ++R+    A E F  T+ 
Sbjct:  458 IYHAVTFGQADIVQFLLKRGAEVNWEGPEDRTPLHVAAAEGNRTITQMLLAFGANVRAKDSAFCTPLHLSVGKLQNLEVSHLLVEHGARILENNIQGVRPVDLEMQLKEMQRLLVQSACEAFYSTDL 584

[Close]

BLAST of SMED30005308 vs. Ensembl Medaka
Match: anks4b (ankyrin repeat and sterile alpha motif domain containing 4B [Source:NCBI gene;Acc:101158199])

HSP 1 Score: 104.375 bits (259), Expect = 5.653e-25
Identity = 55/147 (37.41%), Postives = 85/147 (57.82%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A      +LL  A+  +  + DE+GM     AA+ G +E L+L+ SR     K D  G T LHHA   NH+ IV +LVN+G  ++ LDNDF++ + +A  + ++EI  FLD  A R+ N + + V  ++ + QK+A +RVK  EK +
Sbjct:    7 AAADGYLDLLKEATRKDLNTADEDGMTPTLLAAFHGHVEALQLICSREGDPNKSDIWGNTPLHHAASNNHMHIVSFLVNFGANLFALDNDFHTAMDVAASHGHMEIVRFLDAAASRQTNQNSRKVANLKKEAQKEAERRVKLCEKVK 153

[Close]

BLAST of SMED30005308 vs. Ensembl Medaka
Match: ush1gb (Usher syndrome type-1G protein homolog [Source:NCBI gene;Acc:101174708])

HSP 1 Score: 104.76 bits (260), Expect = 7.027e-25
Identity = 50/147 (34.01%), Postives = 84/147 (57.14%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFR 481
            A +  + +LL  A+  E    DE+GM    WAAY G+LE L+L++SRG    + D  G T LH A    H   + +LV +G  +W LDND+++PL +A    +++   +LD+ A ++   +PK V +++ +  + A +R+K  EK +
Sbjct:    8 AARDGHLDLLREATRKELNGPDEDGMTPTLWAAYHGNLEALRLIVSRGGDPDRCDIWGNTPLHLAAANGHQNCLSFLVAFGANVWCLDNDYHTPLDMAASKGHMDCVRYLDSIAAKQTTLNPKVVSKLKERAFRSAERRIKDCEKLQ 154

[Close]

BLAST of SMED30005308 vs. Ensembl Medaka
Match: ush1ga (Usher syndrome type-1G protein homolog [Source:NCBI gene;Acc:101169538])

HSP 1 Score: 103.219 bits (256), Expect = 2.391e-24
Identity = 48/141 (34.04%), Postives = 86/141 (60.99%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVK 463
            A +    +LL  A+  +  + DE+GM    WAAY G+LE L+L+++RG +  K D  G T LH A    H+  + +LV++G  IW LDND+++PL +A +  +++   +LD+ A ++   +PK V +++ +  ++A +R+K
Sbjct:    8 AARDGYLDLLKEATRKDLNAPDEDGMTPTLWAAYHGNLEALRLIVARGGNPDKCDIWGNTPLHLAAANGHLNCLFFLVSFGANIWCLDNDYHTPLDMAAMKNHMDCVRYLDSIAAKQTGLNPKLVGKLKDRAFREAERRIK 148

[Close]

BLAST of SMED30005308 vs. Ensembl Medaka
Match: ehmt2 (euchromatic histone lysine methyltransferase 2 [Source:NCBI gene;Acc:101167716])

HSP 1 Score: 62.7734 bits (151), Expect = 1.479e-10
Identity = 34/97 (35.05%), Postives = 55/97 (56.70%), Query Frame = 2

Query:  107 ENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +N  +++H AA  G LEI  +++  G  +   D   RT L  AVI NH+E+ RYLV  G C++++++D  + L  A    N+EI + L    Q ++N
Sbjct:  615 QNRRSALHTAAQRGLLEICYMLIQAGAHVDAKDKEMRTPLLEAVINNHIEVARYLVQNGACVYHVEDDGYTGLHHAAKLGNLEIVNMLLETGQVDIN 711

HSP 2 Score: 56.9954 bits (136), Expect = 1.168e-8
Identity = 33/110 (30.00%), Postives = 62/110 (56.36%), Query Frame = 2

Query:   98 SVDENGMN-SIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERV 424
            S+ +  MN  +HWAAY G ++I ++VL+ GCS+  ++ +G T LH A    ++E V   ++ G  I  ++ + ++PL LA  +  + +S  ++   +R + N     ER+
Sbjct:  744 SITDKEMNVCLHWAAYAGSVDIAEMVLNAGCSLSSVNVHGDTPLHIAAREGYLECVTLFLSRGADIDIVNKEGDTPLTLARTDTPVWVSLQINRKLRRGITNRLLRTERI 853

HSP 3 Score: 49.6766 bits (117), Expect = 2.381e-6
Identity = 33/102 (32.35%), Postives = 54/102 (52.94%), Query Frame = 2

Query:  101 VDENGMNSIHWAAYLGDLEILKLVLSRG-CSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDN--CAQREVN 397
            V+++G   +H AA LG+LEI+ ++L  G   I   DN G T +  A    HVE+++ L+N G  +   D + N  L  A    +++I+  + N  C+   VN
Sbjct:  679 VEDDGYTGLHHAAKLGNLEIVNMLLETGQVDINAQDNGGWTPIIWAAEHKHVEVIKALLNRGADVSITDKEMNVCLHWAAYAGSVDIAEMVLNAGCSLSSVN 780

[Close]

BLAST of SMED30005308 vs. Ensembl Medaka
Match: ehmt2 (euchromatic histone lysine methyltransferase 2 [Source:NCBI gene;Acc:101167716])

HSP 1 Score: 62.3882 bits (150), Expect = 1.887e-10
Identity = 34/97 (35.05%), Postives = 55/97 (56.70%), Query Frame = 2

Query:  107 ENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVN 397
            +N  +++H AA  G LEI  +++  G  +   D   RT L  AVI NH+E+ RYLV  G C++++++D  + L  A    N+EI + L    Q ++N
Sbjct:  661 QNRRSALHTAAQRGLLEICYMLIQAGAHVDAKDKEMRTPLLEAVINNHIEVARYLVQNGACVYHVEDDGYTGLHHAAKLGNLEIVNMLLETGQVDIN 757

HSP 2 Score: 56.6102 bits (135), Expect = 1.486e-8
Identity = 33/110 (30.00%), Postives = 62/110 (56.36%), Query Frame = 2

Query:   98 SVDENGMN-SIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERV 424
            S+ +  MN  +HWAAY G ++I ++VL+ GCS+  ++ +G T LH A    ++E V   ++ G  I  ++ + ++PL LA  +  + +S  ++   +R + N     ER+
Sbjct:  790 SITDKEMNVCLHWAAYAGSVDIAEMVLNAGCSLSSVNVHGDTPLHIAAREGYLECVTLFLSRGADIDIVNKEGDTPLTLARTDTPVWVSLQINRKLRRGITNRLLRTERI 899

HSP 3 Score: 49.6766 bits (117), Expect = 2.991e-6
Identity = 33/102 (32.35%), Postives = 54/102 (52.94%), Query Frame = 2

Query:  101 VDENGMNSIHWAAYLGDLEILKLVLSRG-CSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDN--CAQREVN 397
            V+++G   +H AA LG+LEI+ ++L  G   I   DN G T +  A    HVE+++ L+N G  +   D + N  L  A    +++I+  + N  C+   VN
Sbjct:  725 VEDDGYTGLHHAAKLGNLEIVNMLLETGQVDINAQDNGGWTPIIWAAEHKHVEVIKALLNRGADVSITDKEMNVCLHWAAYAGSVDIAEMVLNAGCSLSSVN 826

[Close]

BLAST of SMED30005308 vs. Planmine SMEST
Match: SMESG000004298.1 (SMESG000004298.1)

HSP 1 Score: 541.962 bits (1395), Expect = 0.000e+0
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 2

Query:   74 MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRDXXXXXXXXXXXXXXXNLEVVKPKSLAQSDTENILLSNNTWTEIELIKSQKEIRTTLKNRTLKKSKNKTSLPDIIVDLEKNSEKIKMDNKSKLFERPGFGSLAFLNKSSLQ 916
            MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRDSKKKKSFEKFTEIFKNLEVVKPKSLAQSDTENILLSNNTWTEIELIKSQKEIRTTLKNRTLKKSKNKTSLPDIIVDLEKNSEKIKMDNKSKLFERPGFGSLAFLNKSSLQ
Sbjct:    1 MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRDSKKKKSFEKFTEIFKNLEVVKPKSLAQSDTENILLSNNTWTEIELIKSQKEIRTTLKNRTLKKSKNKTSLPDIIVDLEKNSEKIKMDNKSKLFERPGFGSLAFLNKSSLQ 281

[Close]

BLAST of SMED30005308 vs. Planmine SMEST
Match: SMESG000004298.1 (SMESG000004298.1)

HSP 1 Score: 362.073 bits (928), Expect = 7.139e-128
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 2

Query:   74 MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRD 586
            MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRD
Sbjct:    1 MASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRD 171

[Close]

BLAST of SMED30005308 vs. Planmine SMEST
Match: SMESG000010564.1 (SMESG000010564.1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.714e-11
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 2

Query:    2 ETFKPKISTYCINAVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            E ++P   ++C  A+   ++E+L +     C  E VD  G   IH+A   G  EILKL+L   C+I+  DN G   +H+A  G H EI++ L+++   I    ND   P++  I ++NI++   L
Sbjct:  151 EGWRP---SHC--AIDGGHSEILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLLLDHNCNIECEDNEGWRPIHYATNGGHSEILKLLLDHNCNIDCETNDGKRPIQFVIESKNIDLLKLL 270

HSP 2 Score: 58.9214 bits (141), Expect = 2.253e-9
Identity = 38/112 (33.93%), Postives = 56/112 (50.00%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            ++   N E+L +     C  E  D  GM  IH+A    +LEILKL+  R C+I   DN G   +H+A+   ++EI++ L+     I +   D  SPL LA  N  +E    L
Sbjct:  852 SISGENFEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLLFDRSCNIDCEDNEGMRPIHYAISCENLEILKLLLQQKCGIDHRRKDGKSPLDLAKENNRVECVQLL 963

HSP 3 Score: 56.225 bits (134), Expect = 1.517e-8
Identity = 39/109 (35.78%), Postives = 58/109 (53.21%), Query Frame = 2

Query:   83 SSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL--DNCAQREVNND 403
            ++ECE  D      IH+A+  G  E +KL+L R C+I+ +DN G   +H+A  G H EIV+ L+++   I   DN+   P   AI   + EI   L   NC    V+N+
Sbjct:   78 NTECE--DNQERRPIHYASSFGVTEAMKLLLDRNCNIECVDNEGLRPIHYATNGGHSEIVKLLLDHNCNIECEDNEGWRPSHCAIDGGHSEILKLLLDHNCNIECVDNE 184

HSP 4 Score: 55.8398 bits (133), Expect = 2.272e-8
Identity = 36/106 (33.96%), Postives = 54/106 (50.94%), Query Frame = 2

Query:   59 TELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            TE + +     C  E VD  G+  IH+A   G  EI+KL+L   C+I+  DN G    H A+ G H EI++ L+++   I  +DN+   P+  A    + EI   L
Sbjct:   99 TEAMKLLLDRNCNIECVDNEGLRPIHYATNGGHSEIVKLLLDHNCNIECEDNEGWRPSHCAIDGGHSEILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLL 204

HSP 5 Score: 55.0694 bits (131), Expect = 3.224e-8
Identity = 36/102 (35.29%), Postives = 56/102 (54.90%), Query Frame = 2

Query:   65 LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            LL    + +CE  D  GM  IH++    + EILKL+L R C+I+  DN G   +H+A+   ++EI++ L +    I   DN+   P+  AI  +N+EI   L
Sbjct:  831 LLDRNCNIDCE--DNRGMRPIHYSISGENFEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLLFDRSCNIDCEDNEGMRPIHYAISCENLEILKLL 930

HSP 6 Score: 54.6842 bits (130), Expect = 4.782e-8
Identity = 35/94 (37.23%), Postives = 49/94 (52.13%), Query Frame = 2

Query:   89 ECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            +CE  D  GM  IH+A     LEI KL+L R C+I   DN G   +H+++ G + EI++ L+     I   DN    P+  AI   N+EI   L
Sbjct:  806 DCE--DNEGMRPIHYAINCQKLEIFKLLLDRNCNIDCEDNRGMRPIHYSISGENFEILKLLLERNCNIECKDNKGMRPIHYAISCVNLEILKLL 897

HSP 7 Score: 54.299 bits (129), Expect = 5.568e-8
Identity = 33/105 (31.43%), Postives = 55/105 (52.38%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQN 349
            A+   N E+L       C  E  +  GM  IH+A   G+LEI KL+L   C+I+  DN G   +H+ +   ++EI++ L+++   I   DN+   P+  +I  +N
Sbjct:  489 AISCENLEVLKSLLDRNCNIECEENKGMRPIHYAISCGNLEIFKLLLDLNCNIECEDNKGMRPIHYVITCGNLEILKLLLDHHCNIECEDNEGMKPIHYSISCEN 593

HSP 8 Score: 53.9138 bits (128), Expect = 9.357e-8
Identity = 34/92 (36.96%), Postives = 49/92 (53.26%), Query Frame = 2

Query:   95 ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            E  D  GM  IH+A    +LE LKL+L R C+I+  DN G   +H+A+   ++EI++ L +    I   DN    P+  AI  +N EI   L
Sbjct:  674 ECKDNKGMRPIHYAISCVNLEKLKLLLDRLCNIECKDNKGMRPIHYAISCGNLEILKLLFDRDCNIECKDNKGMRPIHYAISYENFEILKLL 765

HSP 9 Score: 53.5286 bits (127), Expect = 1.119e-7
Identity = 35/100 (35.00%), Postives = 50/100 (50.00%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLA 334
            A+   N E L +     C  E  D  GM  IH+A   G+LEILKL+  R C+I+  DN G   +H+A+   + EI++ L+     I   DN    P+  A
Sbjct:  687 AISCVNLEKLKLLLDRLCNIECKDNKGMRPIHYAISCGNLEILKLLFDRDCNIECKDNKGMRPIHYAISYENFEILKLLLELNCNIECKDNKGMRPIHYA 786

HSP 10 Score: 51.9878 bits (123), Expect = 3.837e-7
Identity = 35/112 (31.25%), Postives = 55/112 (49.11%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            A+   N E+L +     C  E  D   M  IH+A    +LE+LK +L R C+I+  +N G   +H+A+   ++EI + L++    I   DN    P+   I   N+EI   L
Sbjct:  456 AISYQNFEILELLLDRNCIIECEDIKRMRPIHYAISCENLEVLKSLLDRNCNIECEENKGMRPIHYAISCGNLEIFKLLLDLNCNIECEDNKGMRPIHYVITCGNLEILKLL 567

HSP 11 Score: 50.0618 bits (118), Expect = 1.408e-6
Identity = 39/117 (33.33%), Postives = 61/117 (52.14%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEI-SSFLD-NC 379
            A+   N+E+L M     C  E  +E+G+  IH+A    + EILKL+L R C+I+  D      +H+A+   + EI+  L++    I   D     P+  AI  +N+E+  S LD NC
Sbjct:  390 AIINENSEILEMLFDRNCNIECQNESGIRPIHFAISYKNFEILKLLLDRKCNIECEDIKKMRPIHYAISYQNFEILELLLDRNCIIECEDIKRMRPIHYAISCENLEVLKSLLDRNC 506

HSP 12 Score: 48.9062 bits (115), Expect = 3.644e-6
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 2

Query:   65 LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            LL    + ECE  D  G    H A   G  EILKL+L   C+I+ +DN G   +H+A    H EI++ L+++   I   DN+   P+  A    + EI   L
Sbjct:  138 LLDHNCNIECE--DNEGWRPSHCAIDGGHSEILKLLLDHNCNIECVDNEGWRPIHYATNEGHSEILKLLLDHNCNIECEDNEGWRPIHYATNGGHSEILKLL 237

HSP 13 Score: 48.1358 bits (113), Expect = 5.529e-6
Identity = 39/114 (34.21%), Postives = 61/114 (53.51%), Query Frame = 2

Query:   41 AVKQSNTE----LLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            A+   N E    LL +  + ECE  D  GM  IH+    G+LEILKL+L   C+I+  DN G   +H+++   ++EI++ L++    I   +N    P+  AI  +N+EI   L
Sbjct:  522 AISCGNLEIFKLLLDLNCNIECE--DNKGMRPIHYVITCGNLEILKLLLDHHCNIECEDNEGMKPIHYSISCENLEILKLLLDLNCSIECEENKGMRPIHYAINCRNLEILKLL 633

[Close]

BLAST of SMED30005308 vs. Planmine SMEST
Match: SMESG000065361.1 (SMESG000065361.1)

HSP 1 Score: 62.7734 bits (151), Expect = 9.488e-11
Identity = 43/138 (31.16%), Postives = 67/138 (48.55%), Query Frame = 2

Query:   95 ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDN-DFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAIKRVKAYEKFRITEFPEQF 505
            +S D  G+   H AA  G LEIL+ +LS GC  +  D +G   + +A    H+++V YL+ YG C   ++  ++ +PL LA +    EI   L +C       DP A+ R      K  +    AY K+ I   P++ 
Sbjct:   44 DSRDNLGLTVTHHAARNGYLEILEAILSYGCKYEVFDLHGLRPISYACALGHLKVVEYLLRYGSCPNRINTIEYLTPLHLAAMYGYAEIVHLLLHCKA-----DPFALNRE----SKTPLDLACAYGKYNIHCQPDEL 172

[Close]

BLAST of SMED30005308 vs. Planmine SMEST
Match: SMESG000043462.1 (SMESG000043462.1)

HSP 1 Score: 62.003 bits (149), Expect = 1.059e-10
Identity = 43/117 (36.75%), Postives = 58/117 (49.57%), Query Frame = 2

Query:   20 ISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            I   C N        LL    + ECE  D+ GM  IHWA+  G LEILKL+L R C+I+  +  GR  +H+A    H EI++ L++    I   D   N P+  A  N + EI   L
Sbjct:   40 IHIVCRNGFLSVVRLLLDCNCNIECE--DKCGMRPIHWASENGHLEILKLLLDRNCNIECENRWGRIPIHYASGNGHSEILKLLLDRNCNIECQDKWGNRPIHWACRNGHSEILKLL 154

HSP 2 Score: 51.9878 bits (123), Expect = 2.411e-7
Identity = 37/112 (33.04%), Postives = 53/112 (47.32%), Query Frame = 2

Query:   41 AVKQSNTELLSMASSSEC--ESVDENGMNSIHWAAYLGDLEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNLDNDFNSPLKLAIVNQNIEISSFL 370
            A    ++E+L +     C  E  D+ G   IHWA   G  EILKL+L R C+I+  + +GR  +H A    H EI++ L+           D  SPL LA    N++    L
Sbjct:  109 ASGNGHSEILKLLLDRNCNIECQDKWGNRPIHWACRNGHSEILKLLLDRNCNIECENKSGRRSVHFASSNGHSEILKLLLQRKCRFDCKTKDGKSPLDLAKQFNNVDCVELL 220

The following BLAST results are available for this feature:

BLAST of SMED30005308 vs. Ensembl Human
BLAST of SMED30005308 vs. Ensembl Celegans
BLAST of SMED30005308 vs. Ensembl Fly
BLAST of SMED30005308 vs. Ensembl Zebrafish
BLAST of SMED30005308 vs. Ensembl Xenopus
BLAST of SMED30005308 vs. Ensembl Mouse
BLAST of SMED30005308 vs. UniProt/SwissProt
BLAST of SMED30005308 vs. TrEMBL
BLAST of SMED30005308 vs. Ensembl Cavefish
BLAST of SMED30005308 vs. Ensembl Sea Lamprey
BLAST of SMED30005308 vs. Ensembl Yeast
BLAST of SMED30005308 vs. Ensembl Nematostella
BLAST of SMED30005308 vs. Ensembl Medaka
BLAST of SMED30005308 vs. Planmine SMEST

BLAST of SMED30005308 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
USH1G	1.117e-25	36.05	USH1 protein network component sans [Source:HGNC S...	[more]
ANKS4B	1.746e-21	35.71	ankyrin repeat and sterile alpha motif domain cont...	[more]
ANKS4B	1.746e-21	35.71	ankyrin repeat and sterile alpha motif domain cont...	[more]
EHMT2	9.335e-11	36.26	euchromatic histone lysine methyltransferase 2 [So...	[more]
EHMT2	9.335e-11	36.26	euchromatic histone lysine methyltransferase 2 [So...	[more]

BLAST of SMED30005308 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
T28D6.4	6.589e-10	29.13	pep chromosome:WBcel235:III:11360456:11366214:1 ge...	[more]

BLAST of SMED30005308 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Sans	8.685e-16	31.72	gene:FBgn0033785 transcript:FBtr0087791	[more]
Sans	8.685e-16	31.72	gene:FBgn0033785 transcript:FBtr0087790	[more]
Mbs	2.532e-9	27.97	gene:FBgn0005536 transcript:FBtr0308212	[more]
Mbs	2.640e-9	27.97	gene:FBgn0005536 transcript:FBtr0308215	[more]
Mbs	2.835e-9	27.97	gene:FBgn0005536 transcript:FBtr0301472	[more]

BLAST of SMED30005308 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
anks4b	3.042e-27	38.57	ankyrin repeat and sterile alpha motif domain cont...	[more]
ush1ga	1.064e-22	32.62	Usher syndrome 1Ga (autosomal recessive) [Source:Z...	[more]
ush1gb	4.637e-22	31.51	Usher syndrome 1Gb (autosomal recessive) [Source:Z...	[more]
ush1gb	5.889e-22	31.29	Usher syndrome 1Gb (autosomal recessive) [Source:Z...	[more]
ush1ga	1.248e-10	31.46	Usher syndrome 1Ga (autosomal recessive) [Source:Z...	[more]

BLAST of SMED30005308 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
med6	1.617e-23	34.01	mediator complex subunit 6 [Source:Xenbase;Acc:XB-...	[more]
med6	1.617e-23	34.01	mediator complex subunit 6 [Source:Xenbase;Acc:XB-...	[more]
anks4b	9.356e-22	34.42	ankyrin repeat and sterile alpha motif domain cont...	[more]
med6	1.215e-11	32.63	mediator complex subunit 6 [Source:Xenbase;Acc:XB-...	[more]
grsf1	1.306e-10	39.39	G-rich RNA sequence binding factor 1 [Source:Xenba...	[more]

BLAST of SMED30005308 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ush1g	1.137e-25	36.05	USH1 protein network component sans [Source:MGI Sy...	[more]
Anks4b	9.175e-22	36.36	ankyrin repeat and sterile alpha motif domain cont...	[more]
Ehmt2	3.956e-11	36.26	euchromatic histone lysine N-methyltransferase 2 [...	[more]
Ehmt2	4.417e-11	33.66	euchromatic histone lysine N-methyltransferase 2 [...	[more]
Ehmt2	4.439e-11	33.66	euchromatic histone lysine N-methyltransferase 2 [...	[more]

BLAST of SMED30005308 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q495M9\|USH1G_HUMAN	5.365e-25	36.05	Usher syndrome type-1G protein OS=Homo sapiens OX=...	[more]
sp\|Q80T11\|USH1G_MOUSE	7.950e-25	36.05	Usher syndrome type-1G protein homolog OS=Mus musc...	[more]
sp\|Q8K3X6\|ANS4B_MOUSE	6.418e-21	36.36	Ankyrin repeat and SAM domain-containing protein 4...	[more]
sp\|Q8N8V4\|ANS4B_HUMAN	8.385e-21	35.71	Ankyrin repeat and SAM domain-containing protein 4...	[more]
sp\|Q5ZLC8\|ANR52_CHICK	1.562e-10	35.58	Serine/threonine-protein phosphatase 6 regulatory ...	[more]

BLAST of SMED30005308 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S3HTP5	5.663e-27	36.77	Usher syndrome type-1G protein OS=Lingula unguis O...	[more]
A0A1S3KI95	1.159e-26	36.77	Usher syndrome type-1G protein-like OS=Lingula ung...	[more]
A0A267DG34	1.239e-26	38.36	ANK_REP_REGION domain-containing protein OS=Macros...	[more]
A0A1I8JLV4	1.277e-26	38.36	ANK_REP_REGION domain-containing protein OS=Macros...	[more]
A0A267GCM3	4.376e-26	38.36	ANK_REP_REGION domain-containing protein OS=Macros...	[more]

BLAST of SMED30005308 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ush1gb	1.087e-24	34.04	Usher syndrome type-1G protein homolog [Source:NCB...	[more]
anks4b	3.743e-23	35.06	ankyrin repeat and sterile alpha motif domain cont...	[more]
ush1ga	2.435e-22	31.91	USH1 protein network component sans [Source:NCBI g...	[more]
ush1gb	4.649e-12	32.14	Usher syndrome type-1G protein homolog [Source:NCB...	[more]
ANKRD52	2.525e-11	32.17	ankyrin repeat domain 52 [Source:HGNC Symbol;Acc:H...	[more]

BLAST of SMED30005308 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSPMAT00000000095.1	4.792e-10	28.57	pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g...	[more]
ENSPMAT00000000093.1	1.560e-9	29.01	pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g...	[more]
ENSPMAT00000003991.1	5.631e-9	26.73	pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1...	[more]
ENSPMAT00000010339.1	7.109e-9	33.67	pep scaffold:Pmarinus_7.0:GL476759:135524:215537:1...	[more]
ENSPMAT00000010338.1	7.114e-9	33.67	pep scaffold:Pmarinus_7.0:GL476759:135524:215537:1...	[more]

BLAST of SMED30005308 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30005308 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO35466	3.148e-14	26.54	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO39546	1.032e-9	30.61	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35987	9.267e-9	32.18	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49564	2.799e-8	41.25	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49269	4.165e-8	28.35	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30005308 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
anks4b	5.653e-25	37.41	ankyrin repeat and sterile alpha motif domain cont...	[more]
ush1gb	7.027e-25	34.01	Usher syndrome type-1G protein homolog [Source:NCB...	[more]
ush1ga	2.391e-24	34.04	Usher syndrome type-1G protein homolog [Source:NCB...	[more]
ehmt2	1.479e-10	35.05	euchromatic histone lysine methyltransferase 2 [So...	[more]
ehmt2	1.887e-10	35.05	euchromatic histone lysine methyltransferase 2 [So...	[more]

BLAST of SMED30005308 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000004298.1	0.000e+0	100.00	SMESG000004298.1	[more]
SMESG000004298.1	7.139e-128	100.00	SMESG000004298.1	[more]
SMESG000010564.1	2.714e-11	32.80	SMESG000010564.1	[more]
SMESG000065361.1	9.488e-11	31.16	SMESG000065361.1	[more]
SMESG000043462.1	1.059e-10	36.75	SMESG000043462.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005308 ID=SMED30005308|Name=SMED30005308|organism=Schmidtea mediterranea sexual|type=transcript|length=916bp
TGAAACTTTCAAACCAAAAATATCCACTTATTGTATAAATGCCGTGAAAC
AATCAAATACGGAGCTGCTGAGTATGGCGAGTAGTTCTGAATGTGAATCA
GTCGACGAAAATGGAATGAATAGTATACATTGGGCCGCATATCTTGGTGA
CTTGGAAATCCTCAAACTCGTTCTTAGTAGAGGATGTTCCATAAAAAAAA
TAGACAACAATGGTCGAACTGTTTTACATCACGCAGTTATTGGAAATCAC
GTAGAAATCGTGCGTTATCTGGTAAACTATGGAATTTGCATATGGAATCT
CGACAACGACTTCAATAGCCCTTTAAAACTTGCCATAGTAAATCAAAACA
TTGAAATATCTTCATTCTTGGACAATTGCGCACAAAGAGAAGTAAATAAC
GATCCGAAAGCTGTTGAAAGGGTTCAGATTAAATGTCAAAAAAAGGCAAT
AAAAAGAGTTAAAGCGTATGAAAAGTTTCGAATAACCGAATTTCCTGAAC
AGTTTCCATACGATTATGACGACAGGACGATATCAAGTCAACTATCCAAT
TTAAGTCAATACAATTTTCCAATACCTACTAGAGATTCCAAGAAAAAGAA
ATCCTTCGAAAAGTTTACTGAAATATTTAAAAATTTGGAAGTTGTTAAAC
CAAAATCTTTAGCCCAATCAGATACGGAAAATATTTTATTATCAAATAAC
ACTTGGACAGAAATCGAATTGATAAAATCTCAAAAAGAAATAAGAACCAC
ACTAAAAAATAGAACATTGAAAAAGTCAAAAAATAAAACTAGCCTTCCAG
ATATTATTGTCGATTTGGAAAAAAATTCAGAAAAGATAAAAATGGACAAT
AAGAGCAAACTTTTTGAAAGACCCGGATTCGGATCATTGGCATTTTTGAA
CAAATCAAGCTTACAG

protein sequence of SMED30005308-orf-1

>SMED30005308-orf-1 ID=SMED30005308-orf-1|Name=SMED30005308-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=305bp
ETFKPKISTYCINAVKQSNTELLSMASSSECESVDENGMNSIHWAAYLGD
LEILKLVLSRGCSIKKIDNNGRTVLHHAVIGNHVEIVRYLVNYGICIWNL
DNDFNSPLKLAIVNQNIEISSFLDNCAQREVNNDPKAVERVQIKCQKKAI
KRVKAYEKFRITEFPEQFPYDYDDRTISSQLSNLSQYNFPIPTRDSKKKK
SFEKFTEIFKNLEVVKPKSLAQSDTENILLSNNTWTEIELIKSQKEIRTT
LKNRTLKKSKNKTSLPDIIVDLEKNSEKIKMDNKSKLFERPGFGSLAFLN
KSSLQ

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000017	photoreceptor neuron
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR036770	Ankyrin_rpt-contain_sf
IPR002110	Ankyrin_rpt
IPR020683	Ankyrin_rpt-contain_dom

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **biological process**
Term	Definition
GO:0007605	sensory perception of sound

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 35..64 e-value: 0.008 score: 25.4 coord: 68..100 e-value: 1.3E-4 score: 31.3 coord: 101..131 e-value: 610.0 score: 6.6
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 35..67 score: 11.06
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 68..100 score: 10.766
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	G3DSA:1.25.40.20		coord: 6..170 e-value: 2.0E-23 score: 85.0
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	SSF48403	Ankyrin repeat	coord: 11..123
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 40..121 e-value: 2.5E-14 score: 53.6
IPR020683	Ankyrin repeat-containing domain	PROSITE	PS50297	ANK_REP_REGION	coord: 35..121 score: 23.71
None	No IPR available	PANTHER	PTHR24161:SF20	ANKYRIN REPEAT AND SAM DOMAIN-CONTAINING PROTEIN 4B	coord: 11..183
None	No IPR available	PANTHER	PTHR24161	FAMILY NOT NAMED	coord: 11..183

Main menu

SMED30005308

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Owlery

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