HECT and RLD domain containing E3 ubiquitin protein ligase 4
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005288 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Match: HERC4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:HGNC Symbol;Acc:HGNC:24521]) HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0 Identity = 398/1080 (36.85%), Postives = 602/1080 (55.74%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-----VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G AS+GQLG+ +++ P+K D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ +K+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V++I CG HT A++ S+G +Y+FGLG GQLG T + P + G+W P I +E+ + VKR++ GG+ S SS +CG PDD R N T VNE + + S + + E+ SSS + S +S +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ S I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE +II Y+ FYI ++ ++ D+ Y W++ ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL +K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ + YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWYPYNGQCLPDID----SEEYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------PNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI 1049 HSP 2 Score: 90.8929 bits (224), Expect = 8.627e-18 Identity = 57/185 (30.81%), Postives = 96/185 (51.89%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLG 613
+ + + K +F +G YGQLG+ K S PQ + + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + ++ACG HT A +S I+S+G GQLG G
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTS-PQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTG 330 HSP 3 Score: 55.4546 bits (132), Expect = 7.239e-7 Identity = 39/135 (28.89%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHD--CNKVKPNQIQMLDAHKLIDASAGYDH-NAVVTNLGLVFTWGKNSFGELG----RDKLSDFSIK 484
+IF +G +GQLG++ N Y P + + S I I CG DHT L K G +++ G + +LGH+ +++ P ++ L + + + G H +A V + G ++++G G+LG ++ S F++K
Sbjct: 210 AIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVK 342
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Match: HERC4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:HGNC Symbol;Acc:HGNC:24521]) HSP 1 Score: 642.499 bits (1656), Expect = 0.000e+0 Identity = 399/1088 (36.67%), Postives = 601/1088 (55.24%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELW-------------LRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G AS+GQLG+ +++ P+K D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ +K+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V++I CG HT A++ S+G +Y+FGLG GQLG T + P + G+W P I +E+ + VKR++ GG+ S SS +CG PDD R N T VNE + + S + + E+ SSS + S +S +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ S I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE +II Y+ FYI ++ ++ D+ Y W L ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL +K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ + YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWYPYNGQCLPDID----SEEYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------PNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI 1057 HSP 2 Score: 90.8929 bits (224), Expect = 8.814e-18 Identity = 57/185 (30.81%), Postives = 96/185 (51.89%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLG 613
+ + + K +F +G YGQLG+ K S PQ + + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + ++ACG HT A +S I+S+G GQLG G
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTS-PQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTG 330 HSP 3 Score: 55.4546 bits (132), Expect = 7.568e-7 Identity = 39/135 (28.89%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHD--CNKVKPNQIQMLDAHKLIDASAGYDH-NAVVTNLGLVFTWGKNSFGELG----RDKLSDFSIK 484
+IF +G +GQLG++ N Y P + + S I I CG DHT L K G +++ G + +LGH+ +++ P ++ L + + + G H +A V + G ++++G G+LG ++ S F++K
Sbjct: 210 AIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVK 342
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Match: AC098582.1 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:8916]) HSP 1 Score: 621.313 bits (1601), Expect = 0.000e+0 Identity = 378/1058 (35.73%), Postives = 595/1058 (56.24%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE-FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S GQ GI + + PQ IS +++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA++ S+G +YAFG G GQLG T + P+ G+W + ++S + +IVK+++ GG+ + + S + D R N H T+ I V ++E + + +T+ + ++L S+ S + F S G++++ T + + +SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W + P+YF K ++ A+ + G + F++ I L KL+++N + Y+TFYIP+I + D++ Y +W + + ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR+NL+GD + +L + LKK +V F GE+A+D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K L+DL EL+PT G+SLQ LLDY D E+ F LNF + E YG + +LI G + V NRQ +VD YV+Y+ +V + + F GF GK L+ F+P EL+ +MVG+ +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GM+ ++I IQ + YLPVAHTCYNLLDLPKY+S+ IL +L A++N EGF+L
Sbjct: 1 MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNV-KCPSPVKGYWAA----HSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAV--DFRTMNQAHYTSLINDETIAVWRQKLSE--HNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYI--TAALKLLEKLYKVNLKVKHV-EYDTFYIPEISNLVDIQEDYLMWFLHQAGMDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA 1042
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Match: HERC3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:HGNC Symbol;Acc:HGNC:4876]) HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0 Identity = 378/1066 (35.46%), Postives = 595/1066 (55.82%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---------FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S GQ GI + + PQ IS +++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA++ S+G +YAFG G GQLG T + P+ G+W + ++S + +IVK+++ GG+ + + S + D R N H T+ I V ++E + + +T+ + ++L S+ S + F S G++++ T + + +SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W + P+YF K ++ A+ + G + F++ I L KL+++N + Y+TFYIP+I + D++ Y +W + + ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR+NL+GD + +L + LKK +V F GE+A+D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K L+DL EL+PT G+SLQ LLDY D E+ F LNF + E YG + +LI G + V NRQ +VD YV+Y+ +V + + F GF GK L+ F+P EL+ +MVG+ +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GM+ ++I IQ + YLPVAHTCYNLLDLPKY+S+ IL +L A++N EGF+L
Sbjct: 1 MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNV-KCPSPVKGYWAA----HSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAV--DFRTMNQAHYTSLINDETIAVWRQKLSE--HNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYI--TAALKLLEKLYKVNLKVKHV-EYDTFYIPEISNLVDIQEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA 1050
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Match: HERC3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:HGNC Symbol;Acc:HGNC:4876]) HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0 Identity = 378/1066 (35.46%), Postives = 595/1066 (55.82%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---------FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S GQ GI + + PQ IS +++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA++ S+G +YAFG G GQLG T + P+ G+W + ++S + +IVK+++ GG+ + + S + D R N H T+ I V ++E + + +T+ + ++L S+ S + F S G++++ T + + +SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W + P+YF K ++ A+ + G + F++ I L KL+++N + Y+TFYIP+I + D++ Y +W + + ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR+NL+GD + +L + LKK +V F GE+A+D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K L+DL EL+PT G+SLQ LLDY D E+ F LNF + E YG + +LI G + V NRQ +VD YV+Y+ +V + + F GF GK L+ F+P EL+ +MVG+ +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GM+ ++I IQ + YLPVAHTCYNLLDLPKY+S+ IL +L A++N EGF+L
Sbjct: 1 MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNV-KCPSPVKGYWAA----HSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAV--DFRTMNQAHYTSLINDETIAVWRQKLSE--HNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYI--TAALKLLEKLYKVNLKVKHV-EYDTFYIPEISNLVDIQEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA 1050
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Match: herc-1 (HECT and RCC domain E3 ubiquitin ligase [Source:UniProtKB/TrEMBL;Acc:W6RY72]) HSP 1 Score: 469.159 bits (1206), Expect = 2.666e-147 Identity = 331/1036 (31.95%), Postives = 520/1036 (50.19%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCN-----KVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDF--SIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNN-FENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK----HPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFF----SSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEIS---EFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDL--EYSYELWLRNVSKIEFSLFD-YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSF---------------HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYIL----NKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
CG HT+FL K+GK++S G+N +LG + P + + K+I SAG H VT+ G VF WG N G+LG + + + + VQ + IVQVA G +H +AL + +F WG G+ + P+ ++ LS +PI + AG + L+ SG +++WG NN G+LG D S+ V + +V + G NH L+ G F++G GQ G E ++ P V DL+GS V++I G HT+A + G Y FGL GQLG I P + + ++G R G + I S C + YP L + K + VM++ E +++S+++M + + +V + +HG+++D +M F Y++ + +K + +++ N +ECLR L+ I + + K + PF + +I AL KW L R+F++ VF AI + +G KF I++ KL IN+ ++ II TFYI ++ DL EY EL ++ +K E + +PFL +G+ K L+ +EA + + QSS +TVRR+ ++ DT++Q+ ++ L+K +VN GEDA D GGV KE F+LV+ ++L DYG+F ES L WF+ + + + QF+ +GRL GL+IYN+ ++ FPL ++K LL+ +L+DL EL+P+ GK L++LL Y+++D ED+F LNF +S G EL+ G +KPVT +NR YV LYV + L N + Q F++GF ++ + L+FF+P EL+E++VG+E+YDW R Y GEY ++H I FW +F +L+ DE++KFL++LTG R+P++G ++ IQP + LPVAHTC+NLLDLP + L +L+I+IE+ EGF L
Sbjct: 48 CGEQHTVFLTKDGKMWSVGSNMDGQLGRGSRTEGSFSIYP--VSWTSSAKIIQISAGRSHTVSVTDDGRVFAWGSNEHGQLGMESKFTWQGTPRRVQEIND--IVQVASGSNHCIALDEGGRVFVWGEQADGRC--------IHRPEPVEQLSGVPIVRVLAGARHCLALSASGAVFTWGQNNSGELGIDDLRPQTSVQ-HVHQMDGLGVVEAACGDNHTVLLTHCGRTFSFGSDALGQCGFGKKVEKRTNPTAVSDLIGSHVTRIAAGACHTIA--ICKGAPYPFGLNSSGQLG----------NGKIMTQSTPRKTDELDHVTGVFAGYHQTFFVRSSGTIEQNEIVGPS--CPMQ-YPIKLDHESLKKLLDEGDALEVMSL--IESVFSSLASMN-----------MSFLYKDERRYNVGFCFERSHGIDMD---QVMEAFMLFDEYPMKRQYMELIVDSLK---IAFQEWSENLKGIECLRVLLMLPWLPIFTENVNVQTIKNVHTPFVDAMCLITGDLRAALEKWWTQLSTRHFRRMVTVFKTAIKAMLKAKKGPRDCHKFILIMQ---------KLSLINKTYH-IIPLETFYIDELNTSVDLKLEYVLELTRQDPNKTELDYWTHFPFLLNGVAKGELIFVEAWLMQT-----LRRQSSLFNTAHGTIIFELPHLELTVRREFIVSDTMNQMAGLSESDLQKPLKVNIAGEDADDAGGVRKEFFILVMRKILQSDYGMFSEDEESHLVWFSGLPSEFCDREQFHQLGRLVGLSIYNHSIVPFPFPLALYKYLLDTPPDLEDLCELSPSEGKGLKMLLSYEEDDVEDVFGLNFCISFNILGETHTTELLSGGTEKPVTNANRDEYVRLYVHHRLELGYNGEIAQQALLFRKGFSESLHSRTLRFFQPCELKEMIVGNENYDWNEFRDILMYRGEYSSSHPTIQAFWKAFFALTDDERRKFLQFLTGSTRIPVSGWEELHAAIQPSAPES--LPVAHTCFNLLDLPNIADDVELLRRLRISIEHTEGFTLA 1019 HSP 2 Score: 108.612 bits (270), Expect = 1.283e-23 Identity = 72/229 (31.44%), Postives = 115/229 (50.22%), Query Frame = 2
Query: 428 GKNSFGELGRDKLSDFSIKLVQSLTGH-------FIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICD 1093
G G+LG +LS+ +I++ + + G + V CG HT+ LTK+ +++S G+N GQLG G + + + S+ I I+AG + +T G +++WGSN +GQLG + +++ P V+ + IV V+ G NH AL G VF WG G+ H P V L G + ++ G H LA + ++G V+ +G G+LGI D
Sbjct: 9 GHTGEGQLGV-RLSESTIQVPERIIGAPNDMDGTAVTSVTCGEQHTVFLTKDGKMWSVGSNMDGQLGRGSRTEGSFSIYPVSWTSSAKIIQISAGRSHTVSVTDDGRVFAWGSNEHGQLGMESKFTWQGTPRRVQEIND--IVQVASGSNHCIALDEGGRVFVWGEQADGRCIHR-------PEPVEQLSGVPIVRVLAGARHCLA-LSASGAVFTWGQNNSGELGIDD 226 HSP 3 Score: 99.3673 bits (246), Expect = 9.442e-21 Identity = 63/222 (28.38%), Postives = 112/222 (50.45%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG 766
+F++G+ +GQLG++ + + P+++ +I+ G +H + L + G+++ G + H +P ++ L ++ AG H ++ G VFTWG+N+ GELG D L S++ V + G +V+ ACG +HT+ LT FS+G++ GQ G G + + P + +L + I AG + + G Y +G N+ GQLG
Sbjct: 115 VFAWGSNEHGQLGMESKFT-WQGTPRRVQEINDIVQVA--SGSNHCIALDEGGRVFVWGEQADGRCIH-----RPEPVEQLSGVPIVRVLAGARHCLALSASGAVFTWGQNNSGELGIDDLRPQTSVQHVHQMDGLGVVEAACGDNHTVLLTHCGRTFSFGSDALGQCGFGKKVEKRTNPTAVSDLIGSHVTRIAAGACHTIAIC-KGAPYPFGLNSSGQLG 327
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Match: herc-1 (HECT and RCC domain E3 ubiquitin ligase [Source:UniProtKB/TrEMBL;Acc:W6RY72]) HSP 1 Score: 469.159 bits (1206), Expect = 2.666e-147 Identity = 331/1036 (31.95%), Postives = 520/1036 (50.19%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCN-----KVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDF--SIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNN-FENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK----HPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFF----SSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEIS---EFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDL--EYSYELWLRNVSKIEFSLFD-YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSF---------------HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYIL----NKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
CG HT+FL K+GK++S G+N +LG + P + + K+I SAG H VT+ G VF WG N G+LG + + + + VQ + IVQVA G +H +AL + +F WG G+ + P+ ++ LS +PI + AG + L+ SG +++WG NN G+LG D S+ V + +V + G NH L+ G F++G GQ G E ++ P V DL+GS V++I G HT+A + G Y FGL GQLG I P + + ++G R G + I S C + YP L + K + VM++ E +++S+++M + + +V + +HG+++D +M F Y++ + +K + +++ N +ECLR L+ I + + K + PF + +I AL KW L R+F++ VF AI + +G KF I++ KL IN+ ++ II TFYI ++ DL EY EL ++ +K E + +PFL +G+ K L+ +EA + + QSS +TVRR+ ++ DT++Q+ ++ L+K +VN GEDA D GGV KE F+LV+ ++L DYG+F ES L WF+ + + + QF+ +GRL GL+IYN+ ++ FPL ++K LL+ +L+DL EL+P+ GK L++LL Y+++D ED+F LNF +S G EL+ G +KPVT +NR YV LYV + L N + Q F++GF ++ + L+FF+P EL+E++VG+E+YDW R Y GEY ++H I FW +F +L+ DE++KFL++LTG R+P++G ++ IQP + LPVAHTC+NLLDLP + L +L+I+IE+ EGF L
Sbjct: 48 CGEQHTVFLTKDGKMWSVGSNMDGQLGRGSRTEGSFSIYP--VSWTSSAKIIQISAGRSHTVSVTDDGRVFAWGSNEHGQLGMESKFTWQGTPRRVQEIND--IVQVASGSNHCIALDEGGRVFVWGEQADGRC--------IHRPEPVEQLSGVPIVRVLAGARHCLALSASGAVFTWGQNNSGELGIDDLRPQTSVQ-HVHQMDGLGVVEAACGDNHTVLLTHCGRTFSFGSDALGQCGFGKKVEKRTNPTAVSDLIGSHVTRIAAGACHTIA--ICKGAPYPFGLNSSGQLG----------NGKIMTQSTPRKTDELDHVTGVFAGYHQTFFVRSSGTIEQNEIVGPS--CPMQ-YPIKLDHESLKKLLDEGDALEVMSL--IESVFSSLASMN-----------MSFLYKDERRYNVGFCFERSHGIDMD---QVMEAFMLFDEYPMKRQYMELIVDSLK---IAFQEWSENLKGIECLRVLLMLPWLPIFTENVNVQTIKNVHTPFVDAMCLITGDLRAALEKWWTQLSTRHFRRMVTVFKTAIKAMLKAKKGPRDCHKFILIMQ---------KLSLINKTYH-IIPLETFYIDELNTSVDLKLEYVLELTRQDPNKTELDYWTHFPFLLNGVAKGELIFVEAWLMQT-----LRRQSSLFNTAHGTIIFELPHLELTVRREFIVSDTMNQMAGLSESDLQKPLKVNIAGEDADDAGGVRKEFFILVMRKILQSDYGMFSEDEESHLVWFSGLPSEFCDREQFHQLGRLVGLSIYNHSIVPFPFPLALYKYLLDTPPDLEDLCELSPSEGKGLKMLLSYEEDDVEDVFGLNFCISFNILGETHTTELLSGGTEKPVTNANRDEYVRLYVHHRLELGYNGEIAQQALLFRKGFSESLHSRTLRFFQPCELKEMIVGNENYDWNEFRDILMYRGEYSSSHPTIQAFWKAFFALTDDERRKFLQFLTGSTRIPVSGWEELHAAIQPSAPES--LPVAHTCFNLLDLPNIADDVELLRRLRISIEHTEGFTLA 1019 HSP 2 Score: 108.612 bits (270), Expect = 1.283e-23 Identity = 72/229 (31.44%), Postives = 115/229 (50.22%), Query Frame = 2
Query: 428 GKNSFGELGRDKLSDFSIKLVQSLTGH-------FIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICD 1093
G G+LG +LS+ +I++ + + G + V CG HT+ LTK+ +++S G+N GQLG G + + + S+ I I+AG + +T G +++WGSN +GQLG + +++ P V+ + IV V+ G NH AL G VF WG G+ H P V L G + ++ G H LA + ++G V+ +G G+LGI D
Sbjct: 9 GHTGEGQLGV-RLSESTIQVPERIIGAPNDMDGTAVTSVTCGEQHTVFLTKDGKMWSVGSNMDGQLGRGSRTEGSFSIYPVSWTSSAKIIQISAGRSHTVSVTDDGRVFAWGSNEHGQLGMESKFTWQGTPRRVQEIND--IVQVASGSNHCIALDEGGRVFVWGEQADGRCIHR-------PEPVEQLSGVPIVRVLAGARHCLA-LSASGAVFTWGQNNSGELGIDD 226 HSP 3 Score: 99.3673 bits (246), Expect = 9.442e-21 Identity = 63/222 (28.38%), Postives = 112/222 (50.45%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG 766
+F++G+ +GQLG++ + + P+++ +I+ G +H + L + G+++ G + H +P ++ L ++ AG H ++ G VFTWG+N+ GELG D L S++ V + G +V+ ACG +HT+ LT FS+G++ GQ G G + + P + +L + I AG + + G Y +G N+ GQLG
Sbjct: 115 VFAWGSNEHGQLGMESKFT-WQGTPRRVQEINDIVQVA--SGSNHCIALDEGGRVFVWGEQADGRCIH-----RPEPVEQLSGVPIVRVLAGARHCLALSASGAVFTWGQNNSGELGIDDLRPQTSVQHVHQMDGLGVVEAACGDNHTVLLTHCGRTFSFGSDALGQCGFGKKVEKRTNPTAVSDLIGSHVTRIAAGACHTIAIC-KGAPYPFGLNSSGQLG 327
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Match: herc-1 (HECT and RCC domain E3 ubiquitin ligase [Source:UniProtKB/TrEMBL;Acc:W6RY72]) HSP 1 Score: 331.257 bits (848), Expect = 5.197e-100 Identity = 212/606 (34.98%), Postives = 335/606 (55.28%), Query Frame = 2
Query: 1466 QTNHGLNIDLTFNIMSQFF----SSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEIS---EFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDL--EYSYELWLRNVSKIEFSLFD-YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSS----------FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMI--FDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYIL----NKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +HG+++D +M F Y++ + +K + +++ N +ECLR L+ I + + K + PF + +I AL KW L R+F++ VF AI + +G KF I++ KL IN+ ++ II TFYI ++ DL EY EL ++ +K E + +PFL +G+ K L+ +EA + + + ++ +TVRR+ ++ DT++Q+ ++ L+K +VN GEDA D GGV KE F+LV+ ++L DYG+F ES L WF+ + F D QF+ +GRL GL+IYN+ ++ FPL ++K LL+ +L+DL EL+P+ GK L++LL Y+++D ED+F LNF +S G EL+ G +KPVT +NR YV LYV + L N + Q F++GF ++ + L+FF+P EL+E++VG+E+YDW R Y GEY ++H I FW +F +L+ DE++KFL++LTG R+P++G ++ IQP + LPVAHTC+NLLDLP + L +L+I+IE+ EGF L
Sbjct: 57 ERSHGIDMD---QVMEAFMLFDEYPMKRQYMELIVDSLK---IAFQEWSENLKGIECLRVLLMLPWLPIFTENVNVQTIKNVHTPFVDAMCLITGDLRAALEKWWTQLSTRHFRRMVTVFKTAIKAMLKAKKGPRDCHKFILIMQ---------KLSLINKTYH-IIPLETFYIDELNTSVDLKLEYVLELTRQDPNKTELDYWTHFPFLLNGVAKGELIFVEAWLMQTLRRQSSLFNTAHGTIIFELPHLELTVRREFIVSDTMNQMAGLSESDLQKPLKVNIAGEDADDAGGVRKEFFILVMRKILQSDYGMFSEDEESHLVWFSGLPSEFCDR-EQFHQLGRLVGLSIYNHSIVPFPFPLALYKYLLDTPPDLEDLCELSPSEGKGLKMLLSYEEDDVEDVFGLNFCISFNILGETHTTELLSGGTEKPVTNANRDEYVRLYVHHRLELGYNGEIAQQALLFRKGFSESLHSRTLRFFQPCELKEMIVGNENYDWNEFRDILMYRGEYSSSHPTIQAFWKAFFALTDDERRKFLQFLTGSTRIPVSGWEELHAAIQPSAPES--LPVAHTCFNLLDLPNIADDVELLRRLRISIEHTEGFTLA 643
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Match: herc-1 (HECT and RCC domain E3 ubiquitin ligase [Source:UniProtKB/TrEMBL;Acc:W6RY72]) HSP 1 Score: 332.028 bits (850), Expect = 8.178e-100 Identity = 215/611 (35.19%), Postives = 335/611 (54.83%), Query Frame = 2
Query: 1466 QTNHGLNIDLTFNIMSQFF----SSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEIS---EFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDL--EYSYELWLRNVSKIEFSLFD-YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSF---------------HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMI--FDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYIL----NKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +HG+++D +M F Y++ + +K + +++ N +ECLR L+ I + + K + PF + +I AL KW L R+F++ VF AI + +G KF I++ KL IN+ ++ II TFYI ++ DL EY EL ++ +K E + +PFL +G+ K L+ +EA + + QSS +TVRR+ ++ DT++Q+ ++ L+K +VN GEDA D GGV KE F+LV+ ++L DYG+F ES L WF+ + F D QF+ +GRL GL+IYN+ ++ FPL ++K LL+ +L+DL EL+P+ GK L++LL Y+++D ED+F LNF +S G EL+ G +KPVT +NR YV LYV + L N + Q F++GF ++ + L+FF+P EL+E++VG+E+YDW R Y GEY ++H I FW +F +L+ DE++KFL++LTG R+P++G ++ IQP + LPVAHTC+NLLDLP + L +L+I+IE+ EGF L
Sbjct: 102 ERSHGIDMD---QVMEAFMLFDEYPMKRQYMELIVDSLK---IAFQEWSENLKGIECLRVLLMLPWLPIFTENVNVQTIKNVHTPFVDAMCLITGDLRAALEKWWTQLSTRHFRRMVTVFKTAIKAMLKAKKGPRDCHKFILIMQ---------KLSLINKTYH-IIPLETFYIDELNTSVDLKLEYVLELTRQDPNKTELDYWTHFPFLLNGVAKGELIFVEAWLMQT-----LRRQSSLFNTAHGTIIFELPHLELTVRREFIVSDTMNQMAGLSESDLQKPLKVNIAGEDADDAGGVRKEFFILVMRKILQSDYGMFSEDEESHLVWFSGLPSEFCDR-EQFHQLGRLVGLSIYNHSIVPFPFPLALYKYLLDTPPDLEDLCELSPSEGKGLKMLLSYEEDDVEDVFGLNFCISFNILGETHTTELLSGGTEKPVTNANRDEYVRLYVHHRLELGYNGEIAQQALLFRKGFSESLHSRTLRFFQPCELKEMIVGNENYDWNEFRDILMYRGEYSSSHPTIQAFWKAFFALTDDERRKFLQFLTGSTRIPVSGWEELHAAIQPSAPES--LPVAHTCFNLLDLPNIADDVELLRRLRISIEHTEGFTLA 688
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Match: herc-1 (HECT and RCC domain E3 ubiquitin ligase [Source:UniProtKB/TrEMBL;Acc:W6RY72]) HSP 1 Score: 323.553 bits (828), Expect = 7.034e-99 Identity = 197/512 (38.48%), Postives = 296/512 (57.81%), Query Frame = 2
Query: 1742 ALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDL--EYSYELWLRNVSKIEFSLFD-YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSF---------------HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMI--FDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYIL----NKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
AL KW L R+F++ VF AI + +G KF I++ KL IN+ ++ II TFYI ++ DL EY EL ++ +K E + +PFL +G+ K L+ +EA + + QSS +TVRR+ ++ DT++Q+ ++ L+K +VN GEDA D GGV KE F+LV+ ++L DYG+F ES L WF+ + F D QF+ +GRL GL+IYN+ ++ FPL ++K LL+ +L+DL EL+P+ GK L++LL Y+++D ED+F LNF +S G EL+ G +KPVT +NR YV LYV + L N + Q F++GF ++ + L+FF+P EL+E++VG+E+YDW R Y GEY ++H I FW +F +L+ DE++KFL++LTG R+P++G ++ IQP + LPVAHTC+NLLDLP + L +L+I+IE+ EGF L
Sbjct: 11 ALEKWWTQLSTRHFRRMVTVFKTAIKAMLKAKKGPRDCHKFILIMQ---------KLSLINKTYH-IIPLETFYIDELNTSVDLKLEYVLELTRQDPNKTELDYWTHFPFLLNGVAKGELIFVEAWLMQT-----LRRQSSLFNTAHGTIIFELPHLELTVRREFIVSDTMNQMAGLSESDLQKPLKVNIAGEDADDAGGVRKEFFILVMRKILQSDYGMFSEDEESHLVWFSGLPSEFCDR-EQFHQLGRLVGLSIYNHSIVPFPFPLALYKYLLDTPPDLEDLCELSPSEGKGLKMLLSYEEDDVEDVFGLNFCISFNILGETHTTELLSGGTEKPVTNANRDEYVRLYVHHRLELGYNGEIAQQALLFRKGFSESLHSRTLRFFQPCELKEMIVGNENYDWNEFRDILMYRGEYSSSHPTIQAFWKAFFALTDDERRKFLQFLTGSTRIPVSGWEELHAAIQPSAPES--LPVAHTCFNLLDLPNIADDVELLRRLRISIEHTEGFTLA 504
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Match: Herc4 (gene:FBgn0035207 transcript:FBtr0072670) HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0 Identity = 361/1066 (33.86%), Postives = 577/1066 (54.13%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGR--DKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVST--PQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKH-----PTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNR--NYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYF----KKFCMVFINAINNEINGFEGQNTKKKFSDII----EMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFH-------------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
++ +G+ S+GQLG+ + P +I + +++++CG HTLFL GK+Y+CG+N++ +LGHD +P I L + +I G H+ +++ G V +WG N G+LG DK K+V+ L +VQ+ACG++H+LALT E++SWG+N GQLG+ D+ P ++ L +P+ +I G SF+++ SG ++ WG NN GQLG + T+ S P +K LR + +V+ G L+ +GGVFT G G YGQLGH N+ +P +VM+L+GS ++Q+ CG HTLA + S G VYAFGLG GQLG T + + + G WV + S + ++ ++++++ GG+ SI+T + KV P+D R +N K +T + + + + + SI + S S LD + F S NHGL++ + ++ Q + DNI ++ + + +E +R +L+ + S+ K + +PF+ + + E P LNKW+ YF + F V I+ I+ ++ + ++ ++ E++ + + L QIN + + ++Y FY P + D++ Y W+ + +F++ +Y F+FD KT L++ + M + +F + V R+NL+ D++ +L +Q LKK ++ F GE+A D GGV KE F+L++ +L+P YG+F+ Y +SRL WF + F+ F IG L GLAIYN +I+L FPL +FKKLL + +L DL +L+P S+Q LLDY +DF+++F L F +S + +G L +G + VTL NRQ +VDLYVD++ NK+VE + F +GF G+ + F+P EL ++VG+E YDW L+ +CEY Y + I WFW +S EKK FL +LTG DR+PI GM +K+ IQP D+ +LPVAHTC+NLLDLP+Y ++ L+ KL AI+ +GF+LV
Sbjct: 7 LYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETN-RSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLM-LPQVVIGPWV---SPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFV--DKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLD-HDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIW----DNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMQAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYF-LIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTP-DERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV 1058 HSP 2 Score: 124.79 bits (312), Expect = 1.961e-28 Identity = 70/195 (35.90%), Postives = 106/195 (54.36%), Query Frame = 2
Query: 551 LTKNSEIFSWGANTCGQLGLGDNFD-MVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGH-NNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPIN 1129
+ E++ WG+ + GQLGLG D + TP QI + ++ + G + + LT +G +Y+ GSN+Y QLG DL T PL++ L+ I+ + G H ALS G V +WG GQLGH + E +P +V L+ V QI CG NH+LA + S G +Y++G +GQLG+ ++ H P+
Sbjct: 1 MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKR---PLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLA-LTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLR 191
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Match: Herc4 (gene:FBgn0035207 transcript:FBtr0072671) HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0 Identity = 361/1066 (33.86%), Postives = 577/1066 (54.13%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGR--DKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVST--PQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKH-----PTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNR--NYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYF----KKFCMVFINAINNEINGFEGQNTKKKFSDII----EMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFH-------------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
++ +G+ S+GQLG+ + P +I + +++++CG HTLFL GK+Y+CG+N++ +LGHD +P I L + +I G H+ +++ G V +WG N G+LG DK K+V+ L +VQ+ACG++H+LALT E++SWG+N GQLG+ D+ P ++ L +P+ +I G SF+++ SG ++ WG NN GQLG + T+ S P +K LR + +V+ G L+ +GGVFT G G YGQLGH N+ +P +VM+L+GS ++Q+ CG HTLA + S G VYAFGLG GQLG T + + + G WV + S + ++ ++++++ GG+ SI+T + KV P+D R +N K +T + + + + + SI + S S LD + F S NHGL++ + ++ Q + DNI ++ + + +E +R +L+ + S+ K + +PF+ + + E P LNKW+ YF + F V I+ I+ ++ + ++ ++ E++ + + L QIN + + ++Y FY P + D++ Y W+ + +F++ +Y F+FD KT L++ + M + +F + V R+NL+ D++ +L +Q LKK ++ F GE+A D GGV KE F+L++ +L+P YG+F+ Y +SRL WF + F+ F IG L GLAIYN +I+L FPL +FKKLL + +L DL +L+P S+Q LLDY +DF+++F L F +S + +G L +G + VTL NRQ +VDLYVD++ NK+VE + F +GF G+ + F+P EL ++VG+E YDW L+ +CEY Y + I WFW +S EKK FL +LTG DR+PI GM +K+ IQP D+ +LPVAHTC+NLLDLP+Y ++ L+ KL AI+ +GF+LV
Sbjct: 7 LYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETN-RSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLM-LPQVVIGPWV---SPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFV--DKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLD-HDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIW----DNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMQAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYF-LIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTP-DERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV 1058 HSP 2 Score: 124.79 bits (312), Expect = 1.961e-28 Identity = 70/195 (35.90%), Postives = 106/195 (54.36%), Query Frame = 2
Query: 551 LTKNSEIFSWGANTCGQLGLGDNFD-MVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGH-NNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPIN 1129
+ E++ WG+ + GQLGLG D + TP QI + ++ + G + + LT +G +Y+ GSN+Y QLG DL T PL++ L+ I+ + G H ALS G V +WG GQLGH + E +P +V L+ V QI CG NH+LA + S G +Y++G +GQLG+ ++ H P+
Sbjct: 1 MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKR---PLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLA-LTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLR 191
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Match: Herc4 (gene:FBgn0035207 transcript:FBtr0310555) HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0 Identity = 364/1070 (34.02%), Postives = 580/1070 (54.21%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQ-----IQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGR--DKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVST--PQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKH-----PTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNR--NYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYF----KKFCMVFINAINNEINGFEGQNTKKKFSDII----EMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLP--CFMAQSSFH----------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
++ +G+ S+GQLG+ + P +I + +++++CG HTLFL GK+Y+CG+N++ +LGHD +P I L + +I G H+ +++ G V +WG N G+LG DK K+V+ L +VQ+ACG++H+LALT E++SWG+N GQLG+ D+ P ++ L +P+ +I G SF+++ SG ++ WG NN GQLG + T+ S P +K LR + +V+ G L+ +GGVFT G G YGQLGH N+ +P +VM+L+GS ++Q+ CG HTLA + S G VYAFGLG GQLG T + + + G WV + S + ++ ++++++ GG+ SI+T + KV P+D R +N K +T + + + + + SI + S S LD + F S NHGL++ + ++ Q + DNI ++ + + +E +R +L+ + S+ K + +PF+ + + E P LNKW+ YF + F V I+ I+ ++ + ++ ++ E++ + + L QIN + + ++Y FY P + D++ Y W+ + +F++ +Y F+FD KT L++ + M + MA S F+ + V R+NL+ D++ +L +Q LKK ++ F GE+A D GGV KE F+L++ +L+P YG+F+ Y +SRL WF + F+ F IG L GLAIYN +I+L FPL +FKKLL + +L DL +L+P S+Q LLDY +DF+++F L F +S + +G L +G + VTL NRQ +VDLYVD++ NK+VE + F +GF G+ + F+P EL ++VG+E YDW L+ +CEY Y + I WFW +S EKK FL +LTG DR+PI GM +K+ IQP D+ +LPVAHTC+NLLDLP+Y ++ L+ KL AI+ +GF+LV
Sbjct: 7 LYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPRMSPFLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETN-RSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLM-LPQVVIGPWV---SPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFV--DKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLD-HDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIW----DNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYF-LIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTP-DERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV 1062 HSP 2 Score: 122.094 bits (305), Expect = 1.186e-27 Identity = 69/197 (35.03%), Postives = 106/197 (53.81%), Query Frame = 2
Query: 551 LTKNSEIFSWGANTCGQLGLGDNFD-MVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLP--LMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGH-NNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPIN 1129
+ E++ WG+ + GQLGLG D + TP QI + ++ + G + + LT +G +Y+ GSN+Y QLG DL T + L++ L+ I+ + G H ALS G V +WG GQLGH + E +P +V L+ V QI CG NH+LA + S G +Y++G +GQLG+ ++ H P+
Sbjct: 1 MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPRMSPFLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLA-LTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLR 196
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Match: Ube3a (gene:FBgn0061469 transcript:FBtr0076186) HSP 1 Score: 228.024 bits (580), Expect = 4.065e-61 Identity = 133/353 (37.68%), Postives = 209/353 (59.21%), Query Frame = 2
Query: 2162 ITVRRDNLLGDTISQLHRATQCH---LKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSL-ECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKF-FKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+TVRRD L+ D + L + LKK V F+GE +DEGGV KE F L+V ++ NP +G+F E+ WFN F+ N QF IG + GLAIYNNV + ++FP+V+++KL+ DL + +P L KSL+ +LDY D E++F F +S + +G+ +++EL+ +G D V N++ +V+LY D++LN N+EQ F F++GF LK F+P E++ L+ GS +D++ L S Y G Y Q I FW+ ++ ++K K L + TG RVP+ G+ +++ I D + LP +HTC+N+L LP+Y+S L ++L AI ++GF ++
Sbjct: 622 LTVRRDQLINDALIGLEMVAMSNPKDLKKQLVVEFVGEQGIDEGGVSKEFFQLIVEEIFNPAFGMFIQQEETNNMWFNATPFE-NGAQFTLIGIIIGLAIYNNVTLAVNFPMVVYRKLIGYCGTFADLSDWSPALYKSLKSMLDYQGQDMEEVFEQTFKISYSDVFGDVVQHELVPNGQDVLVGQHNKELFVNLYSDFLLNTNIEQQFNAFRKGFEMVTDESPLKLLFRPEEIEMLVCGSREFDFVELENSTVYEGGYTEKSQYIQDFWSIVHAMPSEDKHKLLEFTTGSARVPVGGLKCLRLLITRHGPDSDRLPTSHTCFNVLLLPEYSSREKLEERLMKAINYSKGFGML 973
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Match: HERC2 (gene:FBgn0031107 transcript:FBtr0301344) HSP 1 Score: 166.007 bits (419), Expect = 7.658e-41 Identity = 98/273 (35.90%), Postives = 152/273 (55.68%), Query Frame = 2
Query: 227 GFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKV--KPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGR-DKLSDFSIKLVQSLTGHFIVQVACGHD--HTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGH-NNFENKSIPCIVMDLLGSKVSQIECGMNHTLAY 1027
G H L L +GK++S G E KLGH KP ++ L A K+ D + G H+A +++ G ++TWG +G LG D + KLV +L G +VQVACG TLALT++ +FSWG G+LG G + + TP +I+ LS + + I G +FS LT +G +++WG +Y +LG + P + LR + +++V+VG H A++ G V+ WG +GQ G N F NK P +V+ L V+++ CG +H++A+
Sbjct: 3081 GGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGS-EGSDTPHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHG-GDQHVRKPQPIGGLRGRRVIHVAVGALHCLAVTDAGQVYAWGDNDHGQQGSGNTFVNKK-PALVIGLDAVFVNRVACGSSHSIAW 3350 HSP 2 Score: 156.377 bits (394), Expect = 7.010e-38 Identity = 93/276 (33.70%), Postives = 142/276 (51.45%), Query Frame = 2
Query: 227 GFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKV---KPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGR-DKLSDFSIKLVQSLTGHFIVQVACGHD--HTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVS 1036
G H L L G++Y+ G E KLGH N++ +P ++ L+ + D + G H+A +T G V TWGK +G LG D KLV++L G+ + +ACG TL +T + ++SWG G+LG G + D P +I++L+ L + + G +FS LT SG +Y+WG ++ +LG + + P V L+ + I+ ++ G H A S G V+TWG GQLG P +V L G + ++ CG HTLA S
Sbjct: 4144 GGKHCLALTTEGEVYAWGEGEDGKLGHG-NRMSYDRPKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGS-DGCKLPYKIESLAGLGVVKVECGSQFSVALTKSGAVYTWGKGDFHRLGHG-SVDHVRRPKKVAALQGKKIISIATGSLHCVACSDSGEVYTWGDNDEGQLGDGTVTAIQRPRLVAALQGKHIVKVTCGSAHTLALSTS 4416 HSP 3 Score: 135.961 bits (341), Expect = 1.302e-31 Identity = 92/298 (30.87%), Postives = 151/298 (50.67%), Query Frame = 2
Query: 227 GFDHTLFLL-KNGKLYSCGNNEFKKLGHDC--NKVKPNQIQMLDAH--KLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI-KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAG--DKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDT 1096
G +LF++ ++GK+Y+CG +LG N P+Q+ +L + K + +G H +T G VF+WG+ G+LG + +LV++L I VACG H+ A++ E+++WG G+LG GDN + P+ + L+ + + G D + LT G ++SWG ++G+LGR + ++ P ++ L +V + G AL+ G V+TWG G Y +LGH ++ P + L G +V + G H LA + G VYA+G GQ G +T
Sbjct: 3026 GGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHNVPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDKPRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLK-PKLVTALAGRRVVQVACGSRDAQTLALTEDGAVFSWGDGDFGKLGRGGSEGSDT-PHEIERLSGIGVVQIECGAQFSLALTRAGEVWTWGKGDYYRLGHGGDQHVRKPQPIGGLRGRRVIHVAVGALHCLA-VTDAGQVYAWGDNDHGQQGSGNT 3320 HSP 4 Score: 118.627 bits (296), Expect = 2.587e-26 Identity = 75/228 (32.89%), Postives = 121/228 (53.07%), Query Frame = 2
Query: 416 VFTWGKNSFGELGRDKLSDFSI-KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSIT--AGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMN--HTLAYMVSTGTVYAFGLGKFGQLG 1084
V WG N +LG K S + Q+++ + +A G ++++ ++++ G T G+LGLG + V P Q+ L +K + +G K + LT G ++SWG G+LG T+ + P +V+ LR + I V+ G +H AA+S G ++TWG G YG+LGH + + P +V L G +V Q+ CG TLA + G V+++G G FG+LG
Sbjct: 2988 VMVWGLNDKEQLGGLKGSKVKVPTFSQTISRLRPIHIAGGSKSLFIVSQDGKVYACGEGTNGRLGLGVTHN-VPLPHQLPVLRQYVVKKVAVHSGGKHALALTLDGKVFSWGEGEDGKLGHGNRTTLDK-PRLVEALRAKKIRDVACGSSHSAAISSQGELYTWGLGEYGRLGHGDNTTQLKPKLVTALAGRRVVQVACGSRDAQTLA-LTEDGAVFSWGDGDFGKLG 3212 HSP 5 Score: 111.309 bits (277), Expect = 4.297e-24 Identity = 87/282 (30.85%), Postives = 134/282 (47.52%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVK--PNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI-KLVQSLTGHFIVQVACGHD--HTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDL---TTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSI--PCIVMDLLGSKVSQIECGMNHTLAYMVST 1039
C H L + N YS G E +LGH + P +I L+ H + G ++A +T G + TWG+ ++ LG D S+ LV +L+ H +V VA G H+LALT +F+WG G+LG G N + P +++L ++ + AG +FS L+ G LY+WG LG L + S+P +V L+ + IV V+V H ALS G VF WG Q+ + ++ + I++ L S I C +L + S+
Sbjct: 623 CEGRHFLAIDSNHNAYSWGTGEDHRLGHGDTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNG-NCNGSLQPILVESLPR--VQRVFAGSQFSVALSSEGQLYTWGKATC--LGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQICPASVSSEPLLRTPILVSLPTFPASGIACTSAQSLVWTQSS 899 HSP 6 Score: 106.301 bits (264), Expect = 1.218e-22 Identity = 80/262 (30.53%), Postives = 138/262 (52.67%), Query Frame = 2
Query: 416 VFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIK--SITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVS-TGTVYAFGLGKFGQLGI--CDTVEICHTAKPING-HWVPLECNNQSKIS 1174
++ WG N G+LG L IK ++L+ VQ+A G A+T + ++F+ G + G+LG+G + D + P + +L ++ +K ++ +G K LT G +Y+WG G+LG SY+ P +V+HL + ++ G H AA++ G V TWG GRYG+LGH + E++ P +V LLG + I CG ++ V+++G G +G+LG D ++ + + + G V +EC +Q ++
Sbjct: 4051 IYGWGHNHRGQLG--GLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGS-DSWAIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDR-PKLVEHLNGMSVADIACGSAHSAAITASGHVLTWGKGRYGRLGHGDSEDQLRPKLVEALLGYRAIDIACGSGDAQTLCITDDDNVWSWGDGDYGKLGRGGSDGCKLPYKIESLAGLGVVKVECGSQFSVA 4308 HSP 7 Score: 90.8929 bits (224), Expect = 6.517e-18 Identity = 64/189 (33.86%), Postives = 101/189 (53.44%), Query Frame = 2
Query: 536 DHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPI--VYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDT 1096
D L+ S I+ WG N GQLG G + TP + LS L + G++ F +TP G L++ G + G+LG + S+ ++P ++ L+ + V V+ G H AL+ +G V+ WG G G+LGH N + P +V L G V+ I CG H+ A + ++G V +G G++G+LG D+
Sbjct: 4040 DWALSWGGASTIYGWGHNHRGQLG-GLEGSRIKTPTPCEALSLLRPVQLAGGEQSLFAVTPDGKLFATGYGSGGRLGVGGSDSW-AIPTLLGSLQHVFVKKVAVNSGGKHCLALTTEGEVYAWGEGEDGKLGHGNRMSYDRPKLVEHLNGMSVADIACGSAHSAA-ITASGHVLTWGKGRYGRLGHGDS 4225 HSP 8 Score: 88.5817 bits (218), Expect = 3.376e-17 Identity = 61/191 (31.94%), Postives = 92/191 (48.17%), Query Frame = 2
Query: 494 SLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQN--HVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFG 1060
S+ GH C H LA+ N +SWG +LG GD + P +I L ++S+ G +S +T G L +WG Y +LG + SLP +V L +V V++G H AL+ +G VF WG G YG+LG+ N P +V L +V ++ G ++A + S G +Y +G
Sbjct: 618 SIAGH------CEGRHFLAIDSNHNAYSWGTGEDHRLGHGDTHAR-AVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHG-NSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESL--PRVQRVFAGSQFSVA-LSSEGQLYTWG 797 HSP 9 Score: 76.2554 bits (186), Expect = 1.818e-13 Identity = 58/205 (28.29%), Postives = 102/205 (49.76%), Query Frame = 2
Query: 488 VQSLTGHF-IVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITA---GDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMN--HTLAYMVSTGTVYAFGLGKFGQLG 1084
V +L+ F I QVAC L L++ ++++W +L + + P ++ +++ SI G F + + YSWG+ +LG T ++P + L + + V G ++ AA++ G + TWG G Y +LGH N +++S+P +V+ L V + G H+LA + S G V+A+G G +G+LG
Sbjct: 565 VPTLSCRFQIKQVACCETQMLILSQEGKLYTW------RLAKPE-----AEPLPMEEVAHDVFISIAGHCEGRHF-LAIDSNHNAYSWGTGEDHRLGHG-DTHARAVPTKIAALEQHCVQSVYCGCSYSAAITCGGNLLTWGRGTYARLGHGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLA-LTSEGLVFAWGDGDYGKLG 755 HSP 10 Score: 67.3958 bits (163), Expect = 8.678e-11 Identity = 89/354 (25.14%), Postives = 159/354 (44.92%), Query Frame = 2
Query: 2180 NLLGDTISQLHRATQCHL---KKSFRVNFMGEDALDEGG--------VMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDD--NLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFM---LNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIK-----IKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAI 3178
++ G + +L TQ L + ++V F+GE D GG + EL VP ++N G E +R C+ + F +G L G+A+ + ++ ++++L E DL E++ L + + D D +F L F S G+++ R + P NR YV L + + L++ EQ K ++G + L F ELQ ++ GS LL+ Y G + + ++ WFW + E+ FLR++ G R+P ++D + +++ N D++LP ++TC+ LL +P+Y+ +++L +KLK AI
Sbjct: 4531 SVFGQMVQKLPLLTQEALALPHRVWKVKFVGESVDDCGGGYSESIAEMCDELQNGSVPLLINTPNGRGEA-GANRDCFLLDPTLSSVLQMNMFRFLGVLMGIAVRTGSPLSINLAEPVWRQLTGEVLRPTDLTEVDRDYVAGLLCIRNMD--DDPKLFTALELPFSTS-SARGHEVPLS-TRYTHISP---RNRAEYVRLALGFRLHEFDEQ-VKAVRDGMSKVIPVPLLSLFSAAELQAMVCGSPDIPLGLLKSVATYKG-FDPSSALVTWFWEVMEEFTNQERSLFLRFVWGRTRLP-RTIADFRGRDFVLQVLEKNPPDHFLPESYTCFFLLKMPRYSCKAVLLEKLKYAI 4873 HSP 11 Score: 56.6102 bits (135), Expect = 1.774e-7 Identity = 59/216 (27.31%), Postives = 105/216 (48.61%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFD--HTLFLLKNGKLYSCGNNEFKKLGH-DCN-KVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNS-FG-ELGRDKLSDFSI-KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLG--DNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPS 721
++ ++G +Y +LG N D S P + +S + ++ G H+L L G +++ G+ ++ KLG+ +CN ++P ++ L ++ AG + +++ G ++TWGK + G +L + S+ +LV SL IV VA H LAL+ + E+F WG N Q+ + ++ TP + +L P I S V T S
Sbjct: 688 NLLTWGRGTYARLG--HGNSDDRSLPTLVVALSDHMVVDVALGSGDAHSLALTSEGLVFAWGDGDYGKLGNGNCNGSLQPILVESLP--RVQRVFAGSQFSVALSSEGQLYTWGKATCLGHQLVERSVQGCSVPRLVSSLQHKRIVDVAVSVAHCLALSSSGEVFGWGRNDSQQICPASVSSEPLLRTPILV-SLPTFPASGIACTSAQSLVWTQS 898
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:ZFIN;Acc:ZDB-GENE-070801-1]) HSP 1 Score: 634.41 bits (1635), Expect = 0.000e+0 Identity = 387/1082 (35.77%), Postives = 589/1082 (54.44%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDF--SIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICH--TAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIIT-ASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCK--------LHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-----VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL----------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G AS+GQLG+ +++ P++ + + + CG HT+FLL++G +Y+CG N+ +LGH+ + KP Q+ LDA ++ S G H + + G VF WG S G+LG + D + V+SL+ I QVACG+ H+LAL + EIFSWG N GQLGLG V +PQ I++L +P I+AG SF LT SG ++ WG N +GQLG P+++K LR Q +VY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS VSQI CG HTLA++ S+G + +FGLG GQLG T C+ + P+ G W + + AE+ Y VKR+Y GG+ S A S D G P D+R+ + I ++ ++ + + I + + + SS + S S +H G++I+ + + + Q +A ++ N+ ++ + + P +E LR +L +L + IPF+ + + + P L W +P F++ ++ + + +K + + +F C LH +NE +II Y+ FYI ++ ++ D+ Y W + V + +L YPF+FD KT L++ +A M + PC + + VRR+N++GD++ L ++ KK +V F+GE+A+D GGV KE FLL++ ++L+P YG+F + ESRL WF F+D + F+ IG + GLAIYN +++L+FPL ++KKLL + L+DL EL P +G+SLQ LLDY ++D E+ F LNF ++ + +G ELI +G D V SNRQ +V+ YVDYI NK+V F F GFH GK L+ F+P ELQ +++G+ +YDW L KS EY GEYW +H I FW F L ++KK+FL +LTG DR+PI GM + + IQP + Y PVAHTC+NLLDLPKYTS++ +++KL AIE+N+GF LV
Sbjct: 1 MLCWGNASFGQLGLGGIDEEVLLEPRRCEFFEGKRVRDVGCGRRHTVFLLEDGTVYTCGCNDLGQLGHEKARKKPEQVVSLDAQNIVAVSCGEAHTLALNDKGQVFAWGLGSDGQLGLSIVEDCLRVPRTVKSLSEVHITQVACGYWHSLALARGGEIFSWGQNKYGQLGLGQQGACVCSPQVIQSLQGIPFGQISAGGAHSFGLTLSGAIFGWGRNKFGQLGLG-DNDDRRFPVLLKTLRSQQVVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSVVSQIACGRQHTLAFIPSSGKIDSFGLGGNGQLG---TRSTCNRISPTPVKGCW---RAHADPVAMDHSEAERSYCVKRIYAGGDQSFAHYALSEDDGP---PLDVRIT--RPAGQIYTLSTELIQKWLNH--GLGRIPAEITDEIDLVFSSASCLNGSFLSSSNPDHYSTSSKCSGVDINTARLLFHKLIQTDHPQISQIIAASLEKNLLPKL--SSSPPDIEALRLYLTLPECPLLCNPNNFTTLTIPFAKAIVSLKDAPIKVLGNWWSRFEPVVFQRLVELYKEVVVYLLR-------MQKLGIPLSEQRIFTCFLDTSLKLLEILHSVNERAGQIIQYDKFYIHELDELIDIRNDYATWFQQQMFSVVMDSQVTLCKYPFVFDAQAKTALLQTDAVIQMQMAVDQAQRQNFTSLFLSGGGVESVNPCLI------LIVRRENVVGDSMEVLRKSKNVDYKKPLKVIFVGEEAVDAGGVRKEFFLLIMKELLHPKYGMFRFHEESRLIWFACKTFED-IDLFHLIGVVCGLAIYNLTIVELNFPLALYKKLLKKTPTLEDLKELMPDVGRSLQQLLDYTEDDLEETFCLNFTITEDNFGATEVVELIPNGTDISVNKSNRQEFVNAYVDYIFNKSVAPQFSAFYAGFHKVCGGKVLELFQPSELQAMVIGNTNYDWKELEKSTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLALVIQPTAGGEQYFPVAHTCFNLLDLPKYTSKNTMQEKLLHAIEHNQGFNLV 1052
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:ZFIN;Acc:ZDB-GENE-090313-139]) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 364/1082 (33.64%), Postives = 578/1082 (53.42%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSII---TASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHG--------LNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQI--QYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVF---------DCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF---------SLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G++ GQLGI + P+ +++++CG H+LFLL +G +Y+CG+N +LGHD +P + LDA K+ S G H+ V G VF WG G+LG + +L++ L H I QV CG+ H +AL+K+ ++F WG N+ GQLGLG +PQ +K+L +P+ I+AG SF L+ SG ++ WG N+ GQLG + ++P +K LR Q +VY+S G+ H AAL+ +GG+FT+G+G GQLGH++ N+ +P VM+L+G++VSQI CG NHTLA++ STG +YAFG GQLG I +AK VP+ I T + + +++ GG+H+ + T L C PDD R+ N +I ++N +E + + + S+ ++ L S N+ F + Q++ G ID FN + F + L ++ + S + Q + P +E +R +LI + + S+ + IP +++ + PS L+ W + F + ++ + + + G + + PVF L KLH++N +R+ YN FYIP I + D++ Y W + I+ +L YPF+ + KT +++ +A+ M + +A++ F + VRR++L+ D + +L LKK +V F GE+A+D GGV KE FLL++ +++NP YG+F Y ES L WF+ F ++ F+ IG + GLAIYN+ ++DLHFPLV++KKLL LDDL EL+PT G+SLQ LL+++ D E+ F LNF ++ E YG EL++ G V +NR+ +V Y+ Y+ + V++ + F GF G+ L F+P EL ++VG+ +Y+W + K+ Y GE+ H + FW F S ++KK+FL +LTG DR+PI+GM+ ++I IQ +++YLPVAHTCYN+LD+P Y ++ LR +L A+E EGF+LV
Sbjct: 2 MLCWGSSGAGQLGIGASEASVFE-PRSCCVFDGRGLKEVACGNQHSLFLLHDGSVYTCGSNSCGQLGHDKGGSRPELVGALDAQKISGVSCGQAHSLAVNEQGQVFAWGAGDGGQLGLGTAEEAVRVPRLIKKLCEHRISQVMCGNHHCIALSKDGQLFVWGENSSGQLGLGKGEPSTLSPQPLKSLCGIPLTQISAGGDHSFALSFSGAVFGWGKNSAGQLGLN-DEQDRAVPCHIKFLRSQKVVYISCGEEHTAALTKEGGLFTFGNGSKGQLGHDSTRNEPLPRRVMELMGTEVSQIACGRNHTLAFVPSTGLIYAFGCNTQGQLG----TGIRGSAKSP----VPVSNIQPLCIGNAYTRTEHCTIAKIHSGGDHNFLLLSTVEGLSC-----PDDFRILNTTK--SIALIN---SESLDSWRMKLHDNSDSSITNDIILLLSSTACWNASF-LDQSDDGHFKTNPKVPGID--FNAVRLLFETLMKPAFARLLEQITKSFESLLIPQLPCSPPDVEAMRIYLILSECPAFQDSKNYITLTIPLAMAILRLDANPSKVLDNWWSLMDCTVFTRLVEMYKSIVVFLLTGGKAL-----------LMPVFLESYTSAALRLLEKLHKVNLKAHRV-EYNHFYIPDIATLVDIQEDYLKWFLRKADIKMRYPPVQGSVTLCAYPFILNAQAKTTVLQTDAELQMQMAVSGANLHNVFMLLTMEPLLARNPFLVLHVRRNHLVSDALRELTIYNDVDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELMNPIYGMFTQYSESNLLWFSDKCFVEH-NWFHLIGIICGLAIYNSTVVDLHFPLVLYKKLLEISPTLDDLKELSPTEGRSLQQLLEFE-GDVEETFCLNFAITREYYGITEVKELVQGGKTIAVDKTNRKEFVQAYLQYVFSDAVQEQYSAFSSGFLKVCGGEILSLFQPSELMAMVVGNNNYNWEEMEKNASYKGEFSATHPTVKMFWEVFHEFSLEKKKQFLLFLTGSDRIPIHGMASLRIVIQSTAAEEHYLPVAHTCYNMLDMPCYQTKETLRQRLTQAVEQYEGFSLV 1046
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Match: si:ch73-190m4.1 (si:ch73-190m4.1 [Source:ZFIN;Acc:ZDB-GENE-090311-56]) HSP 1 Score: 371.703 bits (953), Expect = 3.646e-111 Identity = 279/927 (30.10%), Postives = 462/927 (49.84%), Query Frame = 2
Query: 488 VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIK---NSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFA--VSTI-----LEISEFCKVILIPFS-VSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN----------VSKIEFSLFDYPFLFDGITK-TNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMK-ELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPV-NVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGF 3196
++ L ++Q+ACG H++ALT + ++F WG N GQLGL +P+ +++L +P+ I+AG SFVL+ SG ++ WG N+ GQLG TT +P +V L+++ IV +S G H AAL+ G VFT+G G +GQLGHN+F+++ P +V +L GSKVSQ+ CG NHTL S +Y+FG G GQLG + V+ VPL + ++ + E Y +++L G NHS + G + PD R I + + E K+I ++ + S + +K + + S+ GL+ L + ++ S++ + T+ +++++ + N N E LR +++ +S + E++E ++ S + ++ S + W+ NL + FKK F I E + KF +I++ +++Q+ NR I F I ++ + D+ + + N + +E Y F +T + +EAKC + + F +TVRR LL D + L+ TQ HL+ + E+ V K + FL V ++ +P LF + WF + + F G L GLA N+ +++L FPL +FKKLLN K +L+D IE NP G+SLQ +L+Y + E+ + L + E +L ++ +T SNR +VD YVD+I N++V++ F+ F+ GF + + ++ F+P EL+ ++VG+E YDW +L+++ Y G + +H +I FW F L+ +EKK FL +LTG +RVPI GMS +K+++ + N +++LP TC++LL LPKY LR K+ AI + GF
Sbjct: 112 LKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTD-RFVPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLGHNSFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVK----------QSVPLPVDQPTEYNDE------YTIQKLVAGENHSFALYFK-ELGNEANPDPTR--------GIMTFDNGMIEQCECGKDPRKAIKDLKKVFSSAASLNGSFLKTRHDGHYQTSEEYCGLDFGLVESSIATL--SRNERLISTVLKVLQESLLPTL--NPNPTGEESLRLYILLPELISHLKSEQKAELTEALASKILNLSPAALRVLEIYWSKLPDDWLKNL-VKLFKKASAKFFGRIIVENVNNVAKTHMLKFVEILQ---------RVYQVCCSANRNIKKRYFIIHEVNNLLDVLQTMNADVNNNWPWFNLTGQIHALEAQRNAYEETFRKLTSFPCIFNLEAKCSYMRNR---VWKGRFELTVRRTALLEDCLCLLNPGTQQHLRDKWLSILFTENVSKRTDVHKRDFFLNVFKELCDPASQLFMYNDNETMIWFPAKLTVEKKKYF-LFGILCGLAFNNSSVVNLPFPLALFKKLLNIKPSLEDFIEFNPVHGRSLQYILEYSNDVLEESSL-----PLTIIWSGSELDLDPAEPEQHITTSNRTKFVDEYVDHIFNQSVKEAFEEFRRGFFRGCEKRLVEMFEPEELRGVLVGNEEYDWNILKQNTTYEGVFNADHPVIISFWEVFDDLTPNEKKSFLLFLTGFERVPILGMSAVKMRVTHLANSTEDHLPETLTCHSLLQLPKYEKRETLRAKVIEAISHKRGF 989 HSP 2 Score: 118.627 bits (296), Expect = 1.923e-26 Identity = 66/213 (30.99%), Postives = 118/213 (55.40%), Query Frame = 2
Query: 335 IQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG--RDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNH-VAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIV 964
++ L+ ++I + G H+ +TN G +F WG+N+ G+LG +++ S + +QSL G + Q++ G +H+ L+ + +F WG N+ GQLGLGD D P + +L I SI+ G + + L G ++++GS +GQLG + + E P +V L + V+ G+NH + + + ++++G G GQLG+ +S+P V
Sbjct: 112 LKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTDRF-VPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLGHN-SFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVKQSVPLPV 322 HSP 3 Score: 104.375 bits (259), Expect = 4.803e-22 Identity = 75/229 (32.75%), Postives = 111/229 (48.47%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLG---HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPS-GFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVY--------VSVGQNHVAAL 871
CG H++ L +G+L+ G N +LG P +Q L + SAG +H+ V++ G+VF WG NS G+LG +D F +V SL IV ++CG +HT AL K +F++G+ GQLG ++F P+ + L + +T G + V T S +YS+G GQLG +S+PL V QP Y + G+NH AL
Sbjct: 125 CGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTDRFVPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLG-HNSFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVK-QSVPLPV----DQPTEYNDEYTIQKLVAGENHSFAL 347 HSP 4 Score: 85.5001 bits (210), Expect = 2.574e-16 Identity = 64/210 (30.48%), Postives = 101/210 (48.10%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNSE-IFSWGANTCGQLGLGDNFDM-VSTP--QQIKNLSNLPIKSITAGDKFSFVL 712
+F +G + GQLG+ S P+ + + + +IS G +H+ L +G ++ G N +LG ++ P + L K++ S G +H A + G VFT+G FG+LG + D +LV L G + QV CG +HTL T + + I+S+G GQLG G+ V P Q + I+ + AG+ SF L
Sbjct: 139 LFVWGENALGQLGLRKEQAGTQS-PRHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGGNSAGQLGLGDTTDRFVPTVVNSLKRKKIVSISCGGEHTAALAKGGTVFTFGSGGFGQLGHNSFKDEHHPRLVAELWGSKVSQVTCGRNHTLVSTASPKLIYSFGCGMQGQLGNGEQVKQSVPLPVDQPTEYNDEYTIQKLVAGENHSFAL 347
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Match: slc47a2.2 (solute carrier family 47 member 2.2 [Source:ZFIN;Acc:ZDB-GENE-070615-14]) HSP 1 Score: 343.969 bits (881), Expect = 5.881e-101 Identity = 295/940 (31.38%), Postives = 458/940 (48.72%), Query Frame = 2
Query: 488 VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNN---QSKISGETTAEQMYI------VKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQS--SDYLQ-TLAVRMKDNIFSQ------IQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIP---KVFDLEYSYELW----------LRNVSKIEFSLFD-------YPFLFDGITKTNL-MKIEAKCLMS--EGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNV--DDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAI 3178
++ L ++Q+ACG H++ALT + ++F WG N GQLGL +PQ +++L +P+ I+AG SFVL+ SG ++ WGSN+ GQLG TT +P +VK L + V +S G H A LS G VFT+G G +GQLGHN+ +++ P +V +L GSKVSQ+ CG +HTL + S+ +Y+FG G GQLG + V+ P+ +P EC + I+GE + ++ ++ G N I+T + + V D W PT + I T S+ S++ + ++ + S GL+ DL ++ +S+S S+ +Q TL + N + + L+ R LI A++ +I E C + +S + + W L + F K I I+ + K F +I++M + C + N D F IP++ + D + LW L N K+E F +PF+ D +TK ++ + ++ +C+ S + L + + HI R+++L DT+ L T + +V F+GED +DE + E F L+ + D + E++ ES L WFN D+ F +G + G+A+YN I++ FPL +FKKLL +K +LDDL EL+P +SL+ LL+ D+ + D+ L+F C G EL+ +G V NRQ YVDLYVD++ NK+V+ F+ F EGF F P ELQEL+ GS Y+W L++S Y G ++ ++I FW F LS + KKKFL +L G +RVP G S +KI + D++LP A TC+ L LPKY+ + LRDKL A+
Sbjct: 110 LKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPQHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTTD-RFIPTIVKSLSGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHNSLKDEHHPRLVAELWGSKVSQVTCGRHHTLVFEDSSNQIYSFGCGMQGQLGNGEQVKQS-VPLPV---LLPTECTEFTMEKLIAGENHSFALFFKSPGNESEKPKSGSNGGILTLNDRIIDRWVSESD----PW--PT--------IKNEINTVFSSAASLNGSFLKTS----------RDEHYQTSVERCGLDFDLVKTSFAKLSTSKSLISEVVQQTLLPSLNPNPTGEEALRLYLLLPELINGLKKQRTKLIEALA--FKILELCPAARKVLEMYWSRLP-------DDWFEALI-KLFHKESAELIKQISRGETDDQSTRHLKNFLEILQM--AYQICCSTNITNHD---------FIIPEVNDLLETLDATSAEVLWMASRLPINEELHNRMKLEDYYFKTAEILVKFPFVSDFLTKKSMFILLQEQCIRSLLDLLVVHLNGNLLHIN--RESVLTDTLQYLRPFTYSFMH-PLQVAFIGEDEIDEKVISAEFFSLISKSFIEWDKKILEVH-ESSLVWFNPHHTQDHR-DFYYLGVICGMALYNRHHINIDFPLALFKKLLQQKPSLDDLEELSPVEARSLKNLLEEDEEEVVDLQHLDFT----CKGQ----ELVPNGSQIQVNKVNRQKYVDLYVDFVFNKSVKTQFEQFTEGFSEGCPLDAWTMFHPEELQELLHGSPQYNWNELQQSASYEGCSASD-ELIKNFWTVFFELSEENKKKFLMFLYGTERVPAGGFSKRALKISQTDCPDPDDHLPEAQTCFERLVLPKYSDINTLRDKLIHAV 985 HSP 2 Score: 120.168 bits (300), Expect = 6.558e-27 Identity = 70/236 (29.66%), Postives = 128/236 (54.24%), Query Frame = 2
Query: 335 IQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG--RDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGG-VFTWGHGRYGQLGHNNFENKSIPCIVM---DLLGSKVSQIECGMNHTLA 1024
++ L+ ++I + G H+ +TN G +F WG+N+ G+LG +++ S + +QSL G + Q++ G +H+ L+ + +F WG+N+ GQLGLGD D P +K+LS SI+ G + + L+ G ++++GS +GQLG + + E P +V L + V+ G++H ++++G G GQLG+ +S+P V+ + + ++ G NH+ A
Sbjct: 110 LKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPQHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTTDRF-IPTIVKSLSGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHN-SLKDEHHPRLVAELWGSKVSQVTCGRHHTLVFEDSSNQIYSFGCGMQGQLGNGEQVKQSVPLPVLLPTECTEFTMEKLIAGENHSFA 343 HSP 3 Score: 111.309 bits (277), Expect = 2.793e-24 Identity = 66/172 (38.37%), Postives = 85/172 (49.42%), Query Frame = 2
Query: 593 CGQLG---LGDN-----FDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLG 1084
CG+ G L D+ D + + +K L N + I GD+ S LT G L+ WG N GQLG + P ++ L P+ +S G NH LS+ G VF WG GQLG + ++ IP IV L G K I CG HT A + GTV+ FG G FGQLG
Sbjct: 84 CGEAGAVLLADDGKVLVMDQSTVCRPLKGLENRQVIQIACGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPQHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTTDRFIPTIVKSLSGKKTVSISCGGEHT-ATLSKGGTVFTFGSGGFGQLG 254 HSP 4 Score: 98.2117 bits (243), Expect = 3.117e-20 Identity = 69/224 (30.80%), Postives = 110/224 (49.11%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLG---HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLT-PSGFLYSWGSNNYGQLGRDLTTSYESLPLMV---KHLRKQPIVYVSVGQNHVAAL 871
CG H++ L +G+L+ G N +LG P +Q L + SAG +H+ V++ G+VF WG NS G+LG +D F +V+SL+G V ++CG +HT L+K +F++G+ GQLG ++ P+ + L + +T G + V S +YS+G GQLG +S+PL V + + + G+NH AL
Sbjct: 123 CGDQHSMALTNDGQLFVWGENALGQLGLRKEQAGTQSPQHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTTDRFIPTIVKSLSGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLG-HNSLKDEHHPRLVAELWGSKVSQVTCGRHHTLVFEDSSNQIYSFGCGMQGQLGNGEQVK-QSVPLPVLLPTECTEFTMEKLIAGENHSFAL 344 HSP 5 Score: 88.5817 bits (218), Expect = 3.229e-17 Identity = 64/210 (30.48%), Postives = 103/210 (49.05%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDNFDMVSTPQQI---KNLSNLPIKSITAGDKFSFVL 712
+F +G + GQLG+ S PQ + + + +IS G +H+ L +G ++ G+N +LG ++ P ++ L K + S G +H A ++ G VFT+G FG+LG + L D +LV L G + QV CG HTL +S +I+S+G GQLG G+ S P + + ++ + AG+ SF L
Sbjct: 137 LFVWGENALGQLGLRKEQAGTQS-PQHLQSLCGIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTTDRFIPTIVKSLSGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHNSLKDEHHPRLVAELWGSKVSQVTCGRHHTLVFEDSSNQIYSFGCGMQGQLGNGEQVKQ-SVPLPVLLPTECTEFTMEKLIAGENHSFAL 344
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Match: si:ch211-281g13.5 (si:ch211-281g13.5 [Source:ZFIN;Acc:ZDB-GENE-090311-7]) HSP 1 Score: 337.806 bits (865), Expect = 8.664e-99 Identity = 297/936 (31.73%), Postives = 462/936 (49.36%), Query Frame = 2
Query: 488 VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNN---QSKISGETTAEQMYI------VKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQS--SDYLQ-TLAVRMKDN------IFSQIQYNRNYPSLECLRFFLIFAV-STILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTK--KKFSDIIEMKPVFDCLCKL-------HQINE--DHNRIIHYN----TFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKI-EAKCLMSEGLPCFMAQSSFH--ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNV--DDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAI 3178
++SL ++Q+ACG H++AL+ + ++F WG NT GQLGL +PQ +++L +P+ I+AG SFVL+ SG ++ WGSN+ GQLG T +P +VK L + V +S G H A LS G VFT+G G +GQLGHN+ +++ P ++ +L GSKVSQ+ CG +HTL + S+ +Y+FG G GQLG + V+ P+ +P EC + I+GE + ++ ++ G N I+T + + V D W PT V I T S+ S++ + ++ + S + GL+ DL ++ F+S+S S+ +Q TL + N + + L+ R LI A+ S ILE++ + +L + + W L + F K I+ I +E + Q+T+ K +I++M C + H+IN+ D + I + Y+ D+ E + R V KI L +PF D ITK + + + +C + +P +SF + + R+++L DT+ L + T + +V F+GED +D G+ E F L+ + D + E++ ES L WFN N F +G + G+A+YN I++ FPL +FKKLL +K +LDDL EL+P +SL+ LL+ D+ + ++ L+F C G EL+ +G V NRQ YVDLYVD NK+V+ F+ F GF F P ELQEL+ GS Y+W L++S Y G ++ ++I FW F LS + KKKFL +L G +RVP G+S +KI + D++LP A TC+ L LPKY+ + L DKL AI
Sbjct: 109 LKSLENRQVIQIACGDQHSMALSNDGQLFVWGENTHGQLGLRKEQAGTQSPQHLQSLCEIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLG-DTKDRFVPTIVKSLCGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHNSLKDEHHPRLIAELWGSKVSQVTCGRHHTLVFEESSKLIYSFGCGMQGQLGNGEQVKQS-VPLPV---LLPTECTEFTMEKLITGEHHSFALFFKNPGNESEKPKSGSNGGILTLNDRMIDRWVSESD----PW--PT--------VKNEINTVFSSAASLNGSFLKTS----------RDEHYQTSVEHCGLDFDLVKTSFAKLFASKSLISEVVQQTLLPSLNPNPTGVEALRLYLLLPELINGLKKQRTKLIEALASKILELNSAARKVLEMYWSRLP----------DDWFERL-IKLFHKESTDLIDQICDETDH---QSTRHLKNILEILQMAYQISCSTDITHHDFIIHKINDLLDLLQTITADLLRMELYMQINEDFHDMVKLEEYYFRTV-KI---LVKFPFASDFITKQRMFNLLQEECFRN--VPNLAMGNSFSNLLHINRESVLTDTLQYLRQNTHSFMH-PLQVAFIGEDGIDMRGLSAEFFSLISKSFIEWDKKILEVH-ESSLVWFNPHHTRGN-KNFYYLGVICGMALYNRHHINIDFPLALFKKLLQQKPSLDDLEELSPVEARSLKNLLEEDEEEVVNLQHLDFT----CKGQ----ELVPNGSQIQVNKVNRQKYVDLYVDLEFNKSVKTQFEQFTRGFSKGCPLDAWTMFHPEELQELLHGSPQYNWKELQQSASYEGCSASD-ELIKNFWTVFFELSEENKKKFLMFLYGTERVPAGGLSKRALKISQTDCPDPDDHLPEAQTCFERLVLPKYSDINTLHDKLIHAI 983 HSP 2 Score: 114.005 bits (284), Expect = 4.835e-25 Identity = 80/256 (31.25%), Postives = 122/256 (47.66%), Query Frame = 2
Query: 320 VKPNQIQMLD-AHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLG 1084
VKP Q++ + K +SA D +A + +G + GK S L ++ +D ++ +Q L I + CG + L + ++ D + + +K+L N + I GD+ S L+ G L+ WG N +GQLG + P ++ L + P+ +S G NH LS+ G VF WG GQLG + +++ +P IV L G K I CG HT A + GTV+ FG G FGQLG
Sbjct: 16 VKPEQVRHGNWGIKFCPSSAVQDISAGKSCIGFIRE-GKVSVLRL-HNEDADEKLEPLQ-LQNERIRSIVCGEAGAVLLADDGKVLI--------------MDQSTVCRPLKSLENRQVIQIACGDQHSMALSNDGQLFVWGENTHGQLGLRKEQAGTQSPQHLQSLCEIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTKDRFVPTIVKSLCGKKTVSISCGGEHT-ATLSKGGTVFTFGSGGFGQLG 253 HSP 3 Score: 111.309 bits (277), Expect = 3.116e-24 Identity = 62/214 (28.97%), Postives = 117/214 (54.67%), Query Frame = 2
Query: 335 IQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG--RDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGG-VFTWGHGRYGQLGHNNFENKSIPCIVM 967
++ L+ ++I + G H+ ++N G +F WG+N+ G+LG +++ S + +QSL + Q++ G +H+ L+ + +F WG+N+ GQLGLGD D P +K+L SI+ G + + L+ G ++++GS +GQLG + + E P ++ L + V+ G++H ++++G G GQLG+ +S+P V+
Sbjct: 109 LKSLENRQVIQIACGDQHSMALSNDGQLFVWGENTHGQLGLRKEQAGTQSPQHLQSLCEIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTKDRF-VPTIVKSLCGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHN-SLKDEHHPRLIAELWGSKVSQVTCGRHHTLVFEESSKLIYSFGCGMQGQLGNGEQVKQSVPLPVL 320 HSP 4 Score: 96.6709 bits (239), Expect = 8.730e-20 Identity = 65/200 (32.50%), Postives = 99/200 (49.50%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLG---HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSG-FLYSWGSNNYGQLGRDLTTSYESLPLMV 808
CG H++ L +G+L+ G N +LG P +Q L + SAG +H+ V++ G+VF WG NS G+LG D F +V+SL G V ++CG +HT L+K +F++G+ GQLG ++ P+ I L + +T G + V S +YS+G GQLG +S+PL V
Sbjct: 122 CGDQHSMALSNDGQLFVWGENTHGQLGLRKEQAGTQSPQHLQSLCEIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTKDRFVPTIVKSLCGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLG-HNSLKDEHHPRLIAELWGSKVSQVTCGRHHTLVFEESSKLIYSFGCGMQGQLGNGEQVK-QSVPLPV 319 HSP 5 Score: 85.8853 bits (211), Expect = 1.960e-16 Identity = 63/210 (30.00%), Postives = 104/210 (49.52%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVK--PNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSD-FSIKLVQSLTGHFIVQVACGHDHTLALTKNSE-IFSWGANTCGQLGLGDNFDMVSTPQQI---KNLSNLPIKSITAGDKFSFVL 712
+F +G ++GQLG+ S PQ + + + +IS G +H+ L +G ++ G+N +LG K + P ++ L K + S G +H A ++ G VFT+G FG+LG + L D +L+ L G + QV CG HTL ++S+ I+S+G GQLG G+ S P + + ++ + G+ SF L
Sbjct: 136 LFVWGENTHGQLGLRKEQAGTQS-PQHLQSLCEIPVAQISAGGNHSFVLSLSGVVFGWGSNSAGQLGLGDTKDRFVPTIVKSLCGKKTVSISCGGEHTATLSKGGTVFTFGSGGFGQLGHNSLKDEHHPRLIAELWGSKVSQVTCGRHHTLVFEESSKLIYSFGCGMQGQLGNGEQVKQ-SVPLPVLLPTECTEFTMEKLITGEHHSFAL 343
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Match: prxl2b (peroxiredoxin like 2B [Source:Xenbase;Acc:XB-GENE-968463]) HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0 Identity = 383/1067 (35.90%), Postives = 594/1067 (55.67%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYG-------GGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEI----NGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-----VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFH--------------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S+GQLG+ +++ P++ D + ++ + CG HT+F+L +G +Y+CG N+ +LGHD ++ KP Q+ LDA ++ S G H + + G VF+WG +G++G ++ I++ ++SL+ IVQVACGH H+LAL+K+S+IFSWG N GQLGLG P+ IK+LS +P I AG SF LT SG ++ WG N +GQLG T ++P ++K LR Q I+++ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+QI CG HTLA++ S+G +Y+FGLG GQLG T+ + + G+W+ E + + G+ + VK ++ G I A + + K D+ + W +N + + + I +V S+ S + N + S G+++++ ++ + Q +A ++ N+ ++ + P +ECLR +LI + + + IPF + + + P L W L+P F K ++ + + + + NG ++ F + L LH++NE +II Y+ FYI ++ D++ Y W++ V+ ++ YPF+FD KT L++ +A M + Q++ + VRRDN++GD + L + KK RV F GE+A+D GGV KE FLL++ ++L+P YG+F Y ESRL WF+ F+D+ F+ IG + GLAIYN +I+LHFPL ++KKLL +K +L+DL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G ELI +G + PV NRQ +VD YVDYI N++V F F GFH K L+ F+P ELQ +++G+ +YDW L KS EY GEYW +H I FW F +L ++KK+FL +LTG DR+PI GM +K+ IQP + +LPVAHTC+NLLDLPKY+ + LR KL AI++ EGF+LV
Sbjct: 1 MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEY-IRVPRNIKSLSDVQIVQVACGHHHSLALSKDSDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETD-RNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINR-KSPFTVKGNWLTYE-DYVRHLPGKKHIHCITTVKGVFSMHFFQNSAGMLEDIVACNCNGIKIRTLDEGVIQRW--------LNYSLGR---LPLEIVNEIDKVFSSAACLNGSFLVTSNNDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQEVAASLEKNLIPRL--TSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIP-LTERRLFYRF--LNTALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMVADPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDS-DLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV 1046
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Match: prxl2b (peroxiredoxin like 2B [Source:Xenbase;Acc:XB-GENE-968463]) HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0 Identity = 383/1064 (36.00%), Postives = 588/1064 (55.26%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK----HPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEI----NGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-----VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFH--------------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S+GQLG+ +++ P++ D + ++ + CG HT+F+L +G +Y+CG N+ +LGHD ++ KP Q+ LDA ++ S G H + + G VF+WG +G++G ++ I++ ++SL+ IVQVACGH H+LAL+K ++IFSWG N GQLGLG P+ IK+LS +P I AG SF LT SG ++ WG N +GQLG T ++P ++K LR Q I+++ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+QI CG HTLA++ S+G +Y+FGLG GQLG T+ + + G+W+ E Y+ R+Y GG+ S ++ +V+ D W NI + I++S + + V N + S G+++++ ++ + Q +A ++ N+ ++ + P +ECLR +LI + + + IPF + + + P L W L+P F K ++ + + + + NG ++ F + L LH++NE +II Y+ FYI ++ D++ Y W++ VS ++ YPF+FD KT L++ +A M + Q++ + VRRDN++GD + L + KK RV F GE+A+D GGV KE FLL++ ++L+P YG+F Y ESRL WF+ F+D+ F+ IG + GLAIYN +I+LHFPL ++KKLL +K +L+DL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G ELI +G + PV NRQ +VD YVDYI N++V F F GFH K L+ F+P ELQ +++G+ +YDW L KS EY GEYW +H I FW F +L ++KK+FL +LTG DR+PI GM +K+ IQP + +LPVAHTC+NLLDLPKY+ + LR KL AI++ EGF+LV
Sbjct: 1 MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEY-IRVPRNIKSLSDVQIVQVACGHHHSLALSKGNDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETD-RNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINR-KSPFTVKGNWLTYE---------------DYV--RIYSGGDQSFAHYITI----QVWQGDCCKMQWAIFAASIVNIYHCYCLIIITIFSSAACLNGSFLVTS-------------NNDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQEVAASLEKNLIPRL--TSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIP-LTERRLFYRF--LNTALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMVSDPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDS-DLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV 1021 HSP 2 Score: 51.6026 bits (122), Expect = 9.494e-6 Identity = 46/172 (26.74%), Postives = 83/172 (48.26%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDC--NKVKPNQIQMLDAHKLIDASAGYDHN-AVVTNLGLVFTWGKNSFGELGR----DKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTC 595
+IF +G +GQLG+ ++ + P + + S I I CG DHT L K G +++ G + +LGH+ ++V P ++ L + + G H A V + G ++++G G+LG ++ S F++K LT V++ G D + A ++ W + C
Sbjct: 210 AIFGWGRNKFGQLGLT--DETDRNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINRKSPFTVK-GNWLTYEDYVRIYSGGDQSFAHYITIQV--WQGDCC 376
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Match: prxl2b (peroxiredoxin like 2B [Source:Xenbase;Acc:XB-GENE-968463]) HSP 1 Score: 628.632 bits (1620), Expect = 0.000e+0 Identity = 379/1068 (35.49%), Postives = 587/1068 (54.96%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEI----NGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-------------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFH--------------ITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S+GQLG+ +++ P++ D + ++ + CG HT+F+L +G +Y+CG N+ +LGHD ++ KP Q+ LDA ++ S G H + + G VF+WG +G++G ++ I++ ++SL+ IVQVACGH H+LAL+K+S+IFSWG N GQLGLG P+ IK+LS +P I AG SF LT SG ++ WG N +GQLG T ++P ++K LR Q I+++ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+QI CG HTLA++ S+G +Y+FGLG GQLG T+ + + G+W+ E + + G+ + VK ++ H +T + C K D+ + W +N + ++ N + S G+++++ ++ + Q +A ++ N+ ++ + P +ECLR +LI + + + IPF + + + P L W L+P F K ++ + + + + NG ++ F + L LH++NE +II Y+ FYI ++ D++ Y W++ V+ ++ YPF+FD KT L++ +A M + Q++ + VRRDN++GD + L + KK RV F GE+A+D GGV KE FLL++ ++L+P YG+F Y ESRL WF+ F+D+ F+ IG + GLAIYN +I+LHFPL ++KKLL +K +L+DL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G ELI +G + PV NRQ +VD YVDYI N++V F F GFH K L+ F+P ELQ +++G+ +YDW L KS EY GEYW +H I FW F +L ++KK+FL +LTG DR+PI GM +K+ IQP + +LPVAHTC+NLLDLPKY+ + LR KL AI++ EGF+LV
Sbjct: 1 MLCWGNTSFGQLGLGGIDEEVVLEPRRCDFFINKNVRDVGCGSRHTVFVLDDGTVYTCGCNDLGQLGHDKSRRKPEQVGALDAQIIVAVSCGEAHTLALNDKGQVFSWGHAVYGQIGLSTAEEY-IRVPRNIKSLSDVQIVQVACGHHHSLALSKDSDIFSWGQNQYGQLGLGLEIKKECAPRHIKSLSGIPFAHIAAGGGHSFALTISGAIFGWGRNKFGQLGLTDETD-RNVPALLKSLRSQKIIHICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTINR-KSPFTVKGNWLTYE-DYVRHLPGKKHIHCITTVKGVFS--MHFFVTQTK--CEKIRTLDEGVIQRW--------LNYSLGRLPLEIVNN-----------------------NDHYMTSSKFSGVDMNVARLLLHKLMDPSQPQIAQEVAASLEKNLIPRL--TSSPPDIECLRLYLILPECPPMADANNLTSLAIPFGTAIVNLEKAPLKVLENWWSLLEPPLFLKVVQLYKDVVVHLLKLSKNGIP-LTERRLFYRFLNT--ALRVLEILHRVNEKAAQIIPYDKFYIHEVQDFVDIQNDYINWIKQQASGMDVNHGLYEVADPPVTICTYPFVFDAQAKTTLLQTDAVIQMQMAVDNAHRQNNLSWLFPATESVDPCLILVVRRDNIVGDAMEVLRKTKNIDYKKPMRVIFFGEEAIDAGGVRKEFFLLIMRELLDPKYGMFRYYEESRLIWFSDKTFEDS-DLFHLIGVVCGLAIYNFTIIELHFPLALYKKLLKKKPSLEDLKELMPIIGRSMQQLLDYTEDDIEETFCLNFTITVENFGTTEVRELIPNGAEIPVNRKNRQDFVDAYVDYIFNQSVASLFSAFSAGFHKVCGSKVLELFQPNELQAMVIGNTAYDWKELEKSTEYKGEYWADHPTIKMFWEIFHALPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYSKKETLRSKLIQAIDHYEGFSLV 1023
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Match: ENSXETT00000039332.1 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:100488790]) HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0 Identity = 369/1056 (34.94%), Postives = 595/1056 (56.34%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK-HPTNITVMNVDVNEPIY---TSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE-FSLFDYPFLFDGITKTNLMKIEAKCLMSEGL---------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G +++GQLG + S P ++ +++++CG HTLFLL++G++YSCG N +LGHD P Q+ L + + G HN ++ G +++WG + G+LG + S ++++ L I QV+CG+DH +AL + ++F+WG N GQLGLG+ F S+PQ++K+L +P+ +TAG SF L+ SG ++ WG+N+ GQLG + E+ P VK LR ++Y+S G+ H A L+ GG+FT+G G GQLGH++ N+ P V++L+GS+VSQI CG HTLA++ S+GT+YAFG G GQLG + A + G W + +IS IVK+++ GGN + + S L+ + DD R + + H + I +DV ++ ++M I + L S S + F S G+N+ + + + S L+ + + + Q+ + + P +E +R ++I +L+ S++ + +P +++ + PS L+ W + P+YF K ++ A+ +NG + F+ I L KLH++N + H +TFYIP+I + D++ Y +W N + ++ +L YPF+FD KT +++ +A+ M + P + + VRR +L+ D + +L + LKK +V F GE+A+D+GGV KE FLL++ ++LNP YG+F +Y +S L WF+ + F ++ F+ IG + GLAIYN ++DL+FPL ++KKLLN + L+DL EL+PT G+SLQ LLDY ++D +D+F LNF + E YG L+ G VT NRQ +VD YV+Y+ N++V++ ++ F GF GK L+ F+P EL+ ++VGS +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GMS ++I IQ ++ +++LPVAHTCYNLLDLPKY+S+ LR +L AI++ EGF+L
Sbjct: 1 MLCWGHSTFGQLGNGHSFQGSVSDPCVCRFSLAACVKEVACGGKHTLFLLEDGQVYSCGWNGEGQLGHDHEGTDPVQVTTLTEEHITHIACGQSHNLALSYQGQLYSWGSGNDGQLGHATVEHSSRIPRIIKKLNQQKIQQVSCGNDHCMALGDDGQLFTWGQNLHGQLGLGNGFLSQSSPQRVKSLEGIPLAQVTAGGSHSFALSLSGAVFGWGNNSAGQLGLNDEKVRET-PCHVKPLRTHKVIYISCGEEHTAILTKAGGLFTFGAGGSGQLGHDSLNNEINPRRVLELMGSEVSQIACGRQHTLAFVPSSGTLYAFGCGDLGQLGTGHKNNVKCPAV-VKGKWAA----HSGQISMHADRAAYNIVKQIFSGGNKTFVLCSKLESSQPA--DDFRFCSQRNHTSVINEETIDVWRQKVLENSNTNSMNGIVQTLSSLACWNGSFLEKRNDEHFRTSPKIPGINMCSIKVLFEKLMNVHHSRLLEQILKGFESFLIPQL--SSSPPDVEAMRIYIILPEFPLLQDSKYYITLTLPLAMAILRLDTNPSKVLDNWWAQVCPKYFLKLISLYKEAVVYLLNGRKTLLIPVLFTSYI--TAALKLLEKLHKVNGKAQHVEH-DTFYIPEISSLVDIQEDYLMWFLNEAGLDSITLCSYPFVFDAQAKTKMLQTDAELQMQVAINGANLQNVFMLLTLDPMLVKNPFLVLHVRRTSLVADALRELSIYSDIDLKKPLKVIFDGEEAVDDGGVTKEFFLLLLKELLNPIYGMFTVYQDSNLLWFSDICFVEH-NWFHLIGIMCGLAIYNFTVVDLYFPLALYKKLLNVQPTLEDLKELSPTEGRSLQELLDYPEDDVDDVFCLNFTICRESYGLAERKPLVAGGDHITVTKDNRQQFVDAYVNYVFNQSVQEWYEAFSTGFLKVCGGKILELFQPSELRSMVVGSNNYNWQELEENAIYKGDYSTAHPTVRMFWETFHDFPLEKKKKFLLFLTGSDRIPIYGMSSLRIIIQSISSGEDHLPVAHTCYNLLDLPKYSSKETLRRRLTQAIDHYEGFSLA 1042
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Match: ENSXETT00000027632.1 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:100488790]) HSP 1 Score: 611.683 bits (1576), Expect = 0.000e+0 Identity = 369/1064 (34.68%), Postives = 595/1064 (55.92%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK-HPTNITVMNVDVNEPIY---TSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF---------SLFDYPFLFDGITKTNLMKIEAKCLMSEGL---------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G +++GQLG + S P ++ +++++CG HTLFLL++G++YSCG N +LGHD P Q+ L + + G HN ++ G +++WG + G+LG + S ++++ L I QV+CG+DH +AL + ++F+WG N GQLGLG+ F S+PQ++K+L +P+ +TAG SF L+ SG ++ WG+N+ GQLG + E+ P VK LR ++Y+S G+ H A L+ GG+FT+G G GQLGH++ N+ P V++L+GS+VSQI CG HTLA++ S+GT+YAFG G GQLG + A + G W + +IS IVK+++ GGN + + S L+ + DD R + + H + I +DV ++ ++M I + L S S + F S G+N+ + + + S L+ + + + Q+ + + P +E +R ++I +L+ S++ + +P +++ + PS L+ W + P+YF K ++ A+ +NG + F+ I L KLH++N + H +TFYIP+I + D++ Y +W N + ++ +L YPF+FD KT +++ +A+ M + P + + VRR +L+ D + +L + LKK +V F GE+A+D+GGV KE FLL++ ++LNP YG+F +Y +S L WF+ + F ++ F+ IG + GLAIYN ++DL+FPL ++KKLLN + L+DL EL+PT G+SLQ LLDY ++D +D+F LNF + E YG L+ G VT NRQ +VD YV+Y+ N++V++ ++ F GF GK L+ F+P EL+ ++VGS +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GMS ++I IQ ++ +++LPVAHTCYNLLDLPKY+S+ LR +L AI++ EGF+L
Sbjct: 1 MLCWGHSTFGQLGNGHSFQGSVSDPCVCRFSLAACVKEVACGGKHTLFLLEDGQVYSCGWNGEGQLGHDHEGTDPVQVTTLTEEHITHIACGQSHNLALSYQGQLYSWGSGNDGQLGHATVEHSSRIPRIIKKLNQQKIQQVSCGNDHCMALGDDGQLFTWGQNLHGQLGLGNGFLSQSSPQRVKSLEGIPLAQVTAGGSHSFALSLSGAVFGWGNNSAGQLGLNDEKVRET-PCHVKPLRTHKVIYISCGEEHTAILTKAGGLFTFGAGGSGQLGHDSLNNEINPRRVLELMGSEVSQIACGRQHTLAFVPSSGTLYAFGCGDLGQLGTGHKNNVKCPAV-VKGKWAA----HSGQISMHADRAAYNIVKQIFSGGNKTFVLCSKLESSQPA--DDFRFCSQRNHTSVINEETIDVWRQKVLENSNTNSMNGIVQTLSSLACWNGSFLEKRNDEHFRTSPKIPGINMCSIKVLFEKLMNVHHSRLLEQILKGFESFLIPQL--SSSPPDVEAMRIYIILPEFPLLQDSKYYITLTLPLAMAILRLDTNPSKVLDNWWAQVCPKYFLKLISLYKEAVVYLLNGRKTLLIPVLFTSYI--TAALKLLEKLHKVNGKAQHVEH-DTFYIPEISSLVDIQEDYLMWFLNEAGLKLRPTIMQDSITLCSYPFVFDAQAKTKMLQTDAELQMQVAINGANLQNVFMLLTLDPMLVKNPFLVLHVRRTSLVADALRELSIYSDIDLKKPLKVIFDGEEAVDDGGVTKEFFLLLLKELLNPIYGMFTVYQDSNLLWFSDICFVEH-NWFHLIGIMCGLAIYNFTVVDLYFPLALYKKLLNVQPTLEDLKELSPTEGRSLQELLDYPEDDVDDVFCLNFTICRESYGLAERKPLVAGGDHITVTKDNRQQFVDAYVNYVFNQSVQEWYEAFSTGFLKVCGGKILELFQPSELRSMVVGSNNYNWQELEENAIYKGDYSTAHPTVRMFWETFHDFPLEKKKKFLLFLTGSDRIPIYGMSSLRIIIQSISSGEDHLPVAHTCYNLLDLPKYSSKETLRRRLTQAIDHYEGFSLA 1050
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Match: Herc4 (hect domain and RLD 4 [Source:MGI Symbol;Acc:MGI:1914595]) HSP 1 Score: 659.833 bits (1701), Expect = 0.000e+0 Identity = 393/1080 (36.39%), Postives = 603/1080 (55.83%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-----VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P++ D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ + G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ IK+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+Q+ CG HT A++ S+G +Y+FGLG GQLG T + P + G+W + + + +E + VKR++ GG+ S SS +CG PDD R ++ + VNE + + S + + E+ SSS + S IS +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE ++I Y+ FYI ++ ++ D+ Y W++ ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW +H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ ++YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWF----SYNGQCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------SDPSKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRNDYINWVQQQAYGVLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI 1049 HSP 2 Score: 92.0485 bits (227), Expect = 3.129e-18 Identity = 68/230 (29.57%), Postives = 109/230 (47.39%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDNFDMVS--------------TPQQIKNLSNLPIKSITAGDKFSF 706
+ + + K +F +G YGQLG+ + S PQ I + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + QVACG HT A +S I+S+G GQLG G + S PQ I + +K I +G SF
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTS-PQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNGQCPQDIGSEDYFCVKRIFSGGDQSF 375
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Match: Herc4 (hect domain and RLD 4 [Source:MGI Symbol;Acc:MGI:1914595]) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 393/1088 (36.12%), Postives = 603/1088 (55.42%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-------------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P++ D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ + G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ IK+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+Q+ CG HT A++ S+G +Y+FGLG GQLG T + P + G+W + + + +E + VKR++ GG+ S SS +CG PDD R ++ + VNE + + S + + E+ SSS + S IS +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE ++I Y+ FYI ++ ++ D+ Y W++ ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW +H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ ++YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWF----SYNGQCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------SDPSKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRNDYINWVQQQAYGVDVSHGVTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI 1057 HSP 2 Score: 91.6633 bits (226), Expect = 3.511e-18 Identity = 68/230 (29.57%), Postives = 109/230 (47.39%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDNFDMVS--------------TPQQIKNLSNLPIKSITAGDKFSF 706
+ + + K +F +G YGQLG+ + S PQ I + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + QVACG HT A +S I+S+G GQLG G + S PQ I + +K I +G SF
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTS-PQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNGQCPQDIGSEDYFCVKRIFSGGDQSF 375
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Match: Herc3 (hect domain and RLD 3 [Source:MGI Symbol;Acc:MGI:1921248]) HSP 1 Score: 623.624 bits (1607), Expect = 0.000e+0 Identity = 380/1066 (35.65%), Postives = 592/1066 (55.53%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---------FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S GQ GI + + PQ +S I++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA + S+G +YAFG G GQLG T + P+ GHW + S++S + +VK+++ GG+ + + S+ + D R N H TN I V + E + + +T+ + ++L S+ S + F S G++++ T + + SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W P+YF K ++ A+ + G + F++ M L KL+++N + Y+ FYIP+I + D++ Y +W + S ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR++L+GD + +L + LKK +V F GE+ +D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K +L+DL EL+PT G+SLQ LLDY D E+ F LNF V E YG + +LI G V NRQ +VD YV+YI +V + + F GF GK L+ F+P EL+ +MVG+ +YDW L ++ Y G+Y + H + FW +F ++KKKFL +LTG DR+PI GM+ ++I IQ + YLPVAHTCYNLLDLPKY+S+ I++ +L A++N EGF+L
Sbjct: 1 MLCWGYWSLGQPGISSNLQGIVAEPQVCRFVSDRSIKEVACGGNHSVFLLEDGEVYTCGVNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQTSPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSVNDEVNPRRVLELMGSEVTQIACGRQHTLALVPSSGLIYAFGCGAKGQLGTGHTCNV-KCPSPVKGHWAA----HSSQLSARADRFKYRVVKQIFSGGDQTFVLCSTYENSSPAV--DFRTVNQTHYTNLINDETIAVWRQKLTE--HNNANTVNGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRILFEKLMHSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLETNPSKVLDNWWSQACPKYFMKLVTLYKGAVLYLLRGRKTFLIPVLFNNY--MTATLKLLEKLYKVNLKVKHV-EYDKFYIPEISSLVDIQEDYLMWFLHQSGMKARPSIMQDAVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNHLVGDALRELSIHSDIDLKKPLKVIFDGEEGVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPSLEDLKELSPTEGRSLQELLDYPGEDIEETFCLNFTVCRESYGVIEQKKLIPGGDRVAVCKDNRQEFVDAYVNYIFQISVHEWYTAFSSGFLKVCGGKVLELFQPAELRAMMVGNSNYDWEELEETAVYRGDYSSTHPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIIIQSTATGEEYLPVAHTCYNLLDLPKYSSKEIMKARLTQALDNYEGFSLA 1050
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Match: Herc3 (hect domain and RLD 3 [Source:MGI Symbol;Acc:MGI:1921248]) HSP 1 Score: 479.174 bits (1232), Expect = 5.609e-152 Identity = 306/904 (33.85%), Postives = 485/904 (53.65%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---------FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQT 2719
+ +G S GQ GI + + PQ +S I++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA + S+G +YAFG G GQLG T + P+ GHW + S++S + +VK+++ GG+ + + S+ + D R N H TN I V + E + + +T+ + ++L S+ S + F S G++++ T + + SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W P+YF K ++ A+ + G + F++ M L KL+++N + Y+ FYIP+I + D++ Y +W + S ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR++L+GD + +L + LKK +V F GE+ +D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K +L+DL EL+PT G+SLQ LLDY D E+ F LNF V E YG + +LI G V NRQ
Sbjct: 1 MLCWGYWSLGQPGISSNLQGIVAEPQVCRFVSDRSIKEVACGGNHSVFLLEDGEVYTCGVNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQTSPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSVNDEVNPRRVLELMGSEVTQIACGRQHTLALVPSSGLIYAFGCGAKGQLGTGHTCNV-KCPSPVKGHWAA----HSSQLSARADRFKYRVVKQIFSGGDQTFVLCSTYENSSPAV--DFRTVNQTHYTNLINDETIAVWRQKLTE--HNNANTVNGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRILFEKLMHSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLETNPSKVLDNWWSQACPKYFMKLVTLYKGAVLYLLRGRKTFLIPVLFNNY--MTATLKLLEKLYKVNLKVKH-VEYDKFYIPEISSLVDIQEDYLMWFLHQSGMKARPSIMQDAVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNHLVGDALRELSIHSDIDLKKPLKVIFDGEEGVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPSLEDLKELSPTEGRSLQELLDYPGEDIEETFCLNFTVCRESYGVIEQKKLIPGGDRVAVCKDNRQA 888
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Match: Herc6 (hect domain and RLD 6 [Source:MGI Symbol;Acc:MGI:1914388]) HSP 1 Score: 386.726 bits (992), Expect = 1.894e-116 Identity = 297/1027 (28.92%), Postives = 510/1027 (49.66%), Query Frame = 2
Query: 227 GFDHTLFLLKNGKLYSCGNNEFKKLGH--DCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESL--PLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMY--IVKRLYGGGNHSIITAS------SLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLN--IDLTFNIMSQFFSSQSSD-YLQTLAVR-MKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEY-SYELWLRN-----VSKIEFSLF-DYPFLFDGITKTNLMKIEAKC-LMSE--GLPCFMAQSSFHI-TVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNA-----IGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKI---QPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGF 3196
G H+L L N ++YSCG+N + +LG D + +P IQ L+ + S G +H+ V + G VF WG S G+LG + + S +++L G I+QV+CGH H+LAL+++ +FSWG N+ GQLGLG N + PQ++K+L +P+ + AG SF L+ +G + WGSN GQL E + P + L+ ++Y+S G H A L+ +G VFT+G GQL + + P ++ + +G +VSQIEC HT+AY+ +TG V + G G HT+ P + P E S I+ AE + VK ++ G + + +T S K+ P+ +R +N+ + + + + + + + G N ID+ + F +++ ++ +L +++ + + Y+ P E L FL+ +I++ + K + F+ + + L +L+ + AI ++I G T++ ++I K + + + ++H+ N + + F I ++ +F+ + + +++R+ + +F D+ F+FD +K LMK ++ LMSE P M+ + I VRR +L+ DT+ QL + L+K V F+ E + GGV E F + ++ +P Y +F +YPE + M F N P+F G L GL+++N +I+L FPL ++KKLLN+K +L+DL EL+ LG++LQ +L+ + D E++ M I Y ++ + +LI DG PV +N++ YV YVDYI N +++ ++ F GF+ ++ F+P EL ++G+ + DW + +Y Y +H I FW +F L+ DEKKKFL +LTGCDR+ + G+ + I + + +DN P + TC+ +LDLPKY+S +++ L++AI N+ GF
Sbjct: 30 GEHHSLLLFSNHRVYSCGDNSWGQLGQRRDQSTERPEPIQALNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSLALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSGQLALSGNKVKEQIYKPHSIGALKNLSVIYISCGYEHTAVLTEEGQVFTFGGNSSGQLQPSPRSGQRGPQLI-EGIGGRVSQIECASYHTIAYVYTTGQVVSLGRGP------------SHTSNPTHQE-APAE---NSDITCLLAAEDLVDIEVKDIFAGAHANFVTTRRVSHTRSTGVSMKILPEIIR----------------INQSLVKKWRAANKRKDREGAKREISLIFSSSACLTASFLKKRDAGENNLIDVDLKLARDVFKKLTTEKWISSLITTCLEEYLLRDLPYSS--PHQEALLVFLLLPECSIMQDPKNWKTLAFEFAKAIHKMGPQSLAFLRTCWASLEVSSLNILVQMLKKAIISQIQ--YGVATEQYITNI---KVLLEVIKEVHKANCQ----LPESAFIINELSGIFNFDAEAGRMFIRHNDLDCTESSDMVVFSDFLFVFDLPSKIKLMKCDSFVKLMSEVMAFPEKMSSPPYLILKVRRSHLVEDTLRQLRQVEDFDLRKQLSVGFINEIRPEAGGVSSEFFHCIFEEMTDPKYEMF-IYPEKG----SSMWFPVN-PKFEKSSYFLFGILCGLSLHNLKVINLPFPLALYKKLLNQKPSLEDLKELSLPLGRNLQEVLNCEAGDIEELHMYFSI-----YWDQKDVDLIPDGISVPVNETNKRDYVSKYVDYIFNISIKTIYEEFHRGFYKVCNWDIIRQFQPEELMTAIIGNATCDWKQFENNSKYKDGYDKSHPTILLFWKAFHDLTLDEKKKFLLFLTGCDRLHVKGLQNEGIVFRCSETFSEEDN--PRSLTCHRMLDLPKYSSMRRMKEALQVAINNSTGF 999 HSP 2 Score: 142.124 bits (357), Expect = 9.564e-34 Identity = 85/258 (32.95%), Postives = 137/258 (53.10%), Query Frame = 2
Query: 323 KPNQIQM-LDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG-RDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQL---GHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQL 1081
KP +++ +L+ A++G H+ ++ + V++ G NS+G+LG R S + +Q+L + V+CG +H++A+ ++F+WGA + GQLG+G+ ++ P +IK L+ + I ++ G S L+ G ++SWG N+ GQLG + +++P VK L P+ V+ G H ALS+ G F WG R GQL G+ E P + L V I CG HT A + G V+ FG GQL
Sbjct: 11 KPRRLKAGTSGIELLQAASGEHHSLLLFSNHRVYSCGDNSWGQLGQRRDQSTERPEPIQALNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSLALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSGQLALSGNKVKEQIYKPHSIGALKNLSVIYISCGYEHT-AVLTEEGQVFTFGGNSSGQL 267 HSP 3 Score: 113.235 bits (282), Expect = 9.271e-25 Identity = 67/203 (33.00%), Postives = 106/203 (52.22%), Query Frame = 2
Query: 482 KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLG-HNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGI 1087
+L +G ++Q A G H+L L N ++S G N+ GQLG + P+ I+ L++L + ++ G + S + G +++WG+ + GQLG +P +K L I+ VS G H ALS DG VF+WG GQLG N +++IP V L G ++Q+ G H+ A + TGT + +G + GQL +
Sbjct: 14 RLKAGTSGIELLQAASGEHHSLLLFSNHRVYSCGDNSWGQLGQRRD-QSTERPEPIQALNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSLALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSL-TGTSFGWGSNRSGQLAL 214
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Match: sp|Q6PAV2|HERC4_MOUSE (Probable E3 ubiquitin-protein ligase HERC4 OS=Mus musculus OX=10090 GN=Herc4 PE=1 SV=2) HSP 1 Score: 655.981 bits (1691), Expect = 0.000e+0 Identity = 393/1088 (36.12%), Postives = 603/1088 (55.42%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-------------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P++ D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ + G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ IK+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+Q+ CG HT A++ S+G +Y+FGLG GQLG T + P + G+W + + + +E + VKR++ GG+ S SS +CG PDD R ++ + VNE + + S + + E+ SSS + S IS +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE ++I Y+ FYI ++ ++ D+ Y W++ ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW +H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ ++YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWF----SYNGQCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------SDPSKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRNDYINWVQQQAYGVDVSHGVTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI 1057 HSP 2 Score: 91.6633 bits (226), Expect = 2.456e-17 Identity = 68/230 (29.57%), Postives = 109/230 (47.39%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDNFDMVS--------------TPQQIKNLSNLPIKSITAGDKFSF 706
+ + + K +F +G YGQLG+ + S PQ I + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + QVACG HT A +S I+S+G GQLG G + S PQ I + +K I +G SF
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTS-PQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNGQCPQDIGSEDYFCVKRIFSGGDQSF 375
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Match: sp|Q5PQN1|HERC4_RAT (Probable E3 ubiquitin-protein ligase HERC4 OS=Rattus norvegicus OX=10116 GN=Herc4 PE=2 SV=1) HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0 Identity = 395/1088 (36.31%), Postives = 600/1088 (55.15%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-------------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P+K D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG ++PQ IK+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+QI CG HT A++ S+G +Y+FGLG GQLG T + P + G+W + + + +E + VKR++ GG+ S SS CG PDD R ++ + VNE + + S + + E+ SSS + S +S +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE ++I Y+ FYI ++ ++ D+ Y W++ ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ ++YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVLEPRKSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLQG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIECQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWF----SYNGQCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQSCGP---PDDFRR------SDPSKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHRLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNERTGQLIQYDKFYIHEVQELIDIRNDYINWVQQQAYGVDVSHGITELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI 1057 HSP 2 Score: 92.0485 bits (227), Expect = 2.125e-17 Identity = 67/230 (29.13%), Postives = 109/230 (47.39%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDNFDMVST--------------PQQIKNLSNLPIKSITAGDKFSF 706
+ + + K +F +G YGQLG+ + S PQ I + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + Q+ACG HT A +S I+S+G GQLG G + S PQ I + +K I +G SF
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGIECQKQTS-PQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNGQCPQDIGSEDYFCVKRIFSGGDQSF 375
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Match: sp|Q5GLZ8|HERC4_HUMAN (Probable E3 ubiquitin-protein ligase HERC4 OS=Homo sapiens OX=9606 GN=HERC4 PE=1 SV=1) HSP 1 Score: 642.499 bits (1656), Expect = 0.000e+0 Identity = 399/1088 (36.67%), Postives = 601/1088 (55.24%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASS-LDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFE---GQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELW-------------LRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G AS+GQLG+ +++ P+K D + + + CG HT+F+L +G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G V+ WG +S G+LG S+ I++ ++SL+ IVQVACG+ H+LAL+K SE+F WG N GQLGLG + ++PQ +K+L +P + AG SFVLT SG ++ WG N +GQLG D Y +P ++K LR Q IVY+ G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V++I CG HT A++ S+G +Y+FGLG GQLG T + P + G+W P I +E+ + VKR++ GG+ S SS +CG PDD R N T VNE + + S + + E+ SSS + S +S +H G++++ + + Q +A ++ N+ ++ + P +E LRF+L ++ S I IPF + + + P L W L+P F K +F + + + ++ + ++ F+ + L LH++NE +II Y+ FYI ++ ++ D+ Y W L ++ I ++ YPF+FD KT L++ +A M + PC + + VRR+N++GD + L + KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F Y +SRL WF+ F+D+ F+ IG + GLAIYN ++DLHFPL ++KKLL +K +LDDL EL P +G+S+Q LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +VD YVDYI NK+V F F GFH GK L F+P ELQ +++G+ +YDW L K+ EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQ + YLPV+HTC+NLLDLPKYT + LR KL AI++NEGF+L+
Sbjct: 1 MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVG-SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVKGNWYPYNGQCLPDID----SEEYFCVKRIFSGGDQSFSHYSSPQNCGP---PDDFRC------PNPTKQIWTVNEALIQKWLSYPSGRFPVEIANEIDGTFSSSGC-LNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKL--TSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHT--ALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLI------LVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDS-DLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI 1057 HSP 2 Score: 90.8929 bits (224), Expect = 4.233e-17 Identity = 57/185 (30.81%), Postives = 96/185 (51.89%), Query Frame = 2
Query: 71 FEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLG 613
+ + + K +F +G YGQLG+ K S PQ + + +++ G H+ L +G ++ G N+F +LG + ++ PN ++ L + K++ G DH A +T G VFT+G +G+LG + S + + + V L G + ++ACG HT A +S I+S+G GQLG G
Sbjct: 147 YHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTS-PQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTG 330 HSP 3 Score: 55.4546 bits (132), Expect = 3.634e-6 Identity = 39/135 (28.89%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHD--CNKVKPNQIQMLDAHKLIDASAGYDH-NAVVTNLGLVFTWGKNSFGELG----RDKLSDFSIK 484
+IF +G +GQLG++ N Y P + + S I I CG DHT L K G +++ G + +LGH+ +++ P ++ L + + + G H +A V + G ++++G G+LG ++ S F++K
Sbjct: 210 AIFGWGRNKFGQLGLNDENDRY--VPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVK 342
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Match: sp|Q15034|HERC3_HUMAN (Probable E3 ubiquitin-protein ligase HERC3 OS=Homo sapiens OX=9606 GN=HERC3 PE=1 SV=1) HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0 Identity = 378/1066 (35.46%), Postives = 595/1066 (55.82%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI--KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTN------ITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---------FSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G S GQ GI + + PQ IS +++++CG +H++FLL++G++Y+CG N +LGH+ KP QI L +I + G H+ +++ G +F+WG S G+LG D +L+Q L I+QV+CG+ H LAL + + F+WG N+ GQLGLG F ++PQ++++L +P+ + AG SF L+ SG ++ WG NN GQLG ES P VK LR Q +VY+S G+ H A L+ GGVFT+G G GQLGH++ ++ P V++L+GS+V+QI CG HTLA++ S+G +YAFG G GQLG T + P+ G+W + ++S + +IVK+++ GG+ + + S + D R N H T+ I V ++E + + +T+ + ++L S+ S + F S G++++ T + + +SQ S L+ + + + Q+ + + P +E +R +LI +L+ S++ + IP +++ + PS L+ W + P+YF K ++ A+ + G + F++ I L KL+++N + Y+TFYIP+I + D++ Y +W + + ++ +L YPF+FD KT +++ +A+ M L +A+S F + VRR+NL+GD + +L + LKK +V F GE+A+D GGV KE FLL++ ++LNP YG+F Y +S L WF+ F ++ F+ IG GLAIYN+ ++DLHFPL ++KKLLN K L+DL EL+PT G+SLQ LLDY D E+ F LNF + E YG + +LI G + V NRQ +VD YV+Y+ +V + + F GF GK L+ F+P EL+ +MVG+ +Y+W L ++ Y G+Y H + FW +F ++KKKFL +LTG DR+PI GM+ ++I IQ + YLPVAHTCYNLLDLPKY+S+ IL +L A++N EGF+L
Sbjct: 1 MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRES-PCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNV-KCPSPVKGYWAA----HSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAV--DFRTMNQAHYTSLINDETIAVWRQKLSE--HNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCLIPQL--SSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYI--TAALKLLEKLYKVNLKVKHV-EYDTFYIPEISNLVDIQEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEH-NWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA 1050
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Match: sp|Q9UII4|HERC5_HUMAN (E3 ISG15--protein ligase HERC5 OS=Homo sapiens OX=9606 GN=HERC5 PE=1 SV=2) HSP 1 Score: 418.698 bits (1075), Expect = 3.214e-127 Identity = 300/1003 (29.91%), Postives = 507/1003 (50.55%), Query Frame = 2
Query: 326 PNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDI-------KNSVFHISQTNHGLNIDLTFNIMSQFFS--SQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGF-EGQNTKKKFSDIIEMKPVFDCLCKLHQINE----------DHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE-------FSLFDYPFLFDGITKT------NLMKIEAK---------CLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLC-WFN-KMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPV-AHTCYNLLDLPKYTSESILRDKLKIAIENNEGFA 3199
P I++ K+ G +H ++++ G F + D S ++ L I+Q+ CG H+LAL+K E+F+WG N GQLG+G F +TPQ +++L+ +P+ I+AG+ S L+ SG +YSWG N GQLG T S + P +++ L Q + +V+ G +H A L+ DG +FT+G G++GQLGHN+ +N+ PC+V +L+G +V+QI CG HTLAY+ G V++FG GK GQLG T + P+ V + + + K+ T+ +++ ++ GGN SI+ LW K + + N+ P + + I++V +I S +T + + L N F +Q + +KDN+ ++ ++ P E L F + ++ IS + +++PF+ + + S+ L ++ L+ F K +F A+ +++ + E ++EM L KLH++N+ + ++H F++ + LW V E FS F PF+F+ ++K L+KIE+K + E F + +F +TVRR++L+ D ++QL + L+K V+F GE D GGV KE F + +++ P+YG+F MYPE C WF K F+ ++ G L GL+++N + +L FPL +FKKLL++ +L+DL EL+P LGK+LQ LLD + ++FE++F ++F V + ++ + LI +G V +N++ YV Y++YI N +V+ ++ F+ GF+ +K F P EL++++VG+ YDW K+ Y Y ++H I FW +F L+ +EKKKFL +LTG DR+ + ++++KI + P+ A TC+++L LPKY++ + + L+ AI NN GF
Sbjct: 88 PKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEY----------DNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDD-PSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRD---QLMPLP---VKVSSSEELKLESHTSEKELIMI----AGGNQSIL-----------------LWIKKENSYV---NLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHS--PPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEM------LKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRY--------LWKMTVDASENVQCCVIFSHF--PFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMF-MYPEGASCMWFPVKPKFEKK--RYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNV----HWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG 1024 HSP 2 Score: 98.5969 bits (244), Expect = 1.959e-19 Identity = 73/245 (29.80%), Postives = 130/245 (53.06%), Query Frame = 2
Query: 68 NFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKL-SDFSIKLVQSLTGHFIVQVACGHDHTLALTKN-SEIFSWGANTCGQLGLGDNFD-MVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSW--GSNNYGQLGRDLTT 781
++ + + K +F++G +GQLG+ R + PQ ++ ++ + +IS G H++ L +G +YS G NE +LG H +K P+ I+ LD K+ + G H+A++T GL+FT+G G+LG + ++ LV L G+ + Q+ACG HTLA + ++FS+G+ GQLG G D ++ P ++ + L ++S T+ + + + + W N+Y L R + T
Sbjct: 147 DYHSLALSKGGELFAWGQNLHGQLGVG-RKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPT 390 HSP 3 Score: 80.1073 bits (196), Expect = 9.234e-14 Identity = 61/201 (30.35%), Postives = 97/201 (48.26%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHD---CNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKL-SDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFV-LTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVK 811
CG H+L L K G+L++ G N +LG + P ++ L L SAG H+ ++ G +++WGKN G+LG S L++ L + VACG H+ LT++ +F++GA GQLG + + P + L + I G + ++ G ++S+GS GQLG T + +PL VK
Sbjct: 145 CGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELR-PCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRD-QLMPLPVK 343
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Match: A0A094ZME9 (Putative E3 ubiquitin-protein ligase HERC4 OS=Schistosoma haematobium OX=6185 GN=MS3_04099 PE=4 SV=1) HSP 1 Score: 721.079 bits (1860), Expect = 0.000e+0 Identity = 442/1173 (37.68%), Postives = 643/1173 (54.82%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLG-DNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG------------RDLTTSYES---------------------------LPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFE-NKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNN--QSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNE-------------PIYTSIS----------------TMKSISEVXXXXXXXXXXXXXDIK----NSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTIL----------EISEFCK-------------------------VILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---FSLFDYPFLFDGITKTNLMKIEAK-----------------CLMSEGLPCFMAQSS-----FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYG-NKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQ-IIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+F++G+ GQL +D + P+ +D + +I+CG+ HT+FL +G++YSCG NEF++LGH + ++ L+ +++I + G HNA +T GL+FTWG NS G+LGR+ D +KLV++L+ H +VQV+ G +HTL LT + +FS+G+N GQLGLG + +S PQQI LS LP++ I AG SFVLT SG LY+WGSNNYGQLG +LT +Y S +P VK LR IVY++ G+ H AL+ DGGVFT+G GRYGQLGHN S+P + + +G+ V+QI CG HTLA + TG ++AFG G QLG+ DT PI GHWV N +S + ++++ + Y+++R++ GG+H+ + A G+ V DDLR WN +I + ++ E PI IS M +S+V + S ++ NS F + HG+++DL + SQ F L+ L ++ D++ S + NYPS+ECLR + AVS +L +S+ K ++L +SV+ + + PS L++W ++PRY KK + N I+ ++ ++ ++ + + +LH++NE+H I YN+FY+P + ++ ++ WL+ + FS DYPF+FD +K ++ IEA+ L+S + M F I VRRDN+L DT+S L A KK RV F E+A+DEGGVMKE FLLV+ +LNP YG+F Y ESR+ WF++ + +N+ F +G L GLAIYN++++DL FPL MF+KLL E LDDL EL+P +G+SLQ LLDYD D D+F LNF V+++ +G NKI L+ DG + VT N+ YV+ YV+Y+ K+ E P+K F++GF G LK F+P ELQ L+VGSE +W LR++ Y G YW+ HQ II ++ + +EKKKFLR+LTGCDRVPI G S +KI IQP N D++LPVAHTC NLLDLP+Y+ + IL KL +AI+ EGF LV
Sbjct: 9 LFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTFIQITCGYAHTVFLTVDGEVYSCGCNEFQQLGHGQAGGQLAKVVALEHYRIISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG-DDTGVKLVRALSDHKVVQVSLGVEHTLVLTDTARVFSFGSNLKGQLGLGFSSEQSISIPQQILCLSGLPVRRIVAGGWHSFVLTVSGSLYAWGSNNYGQLGLSYFGSSPKDHQNELTLTYGSVGSTSVDDEQSKDRPFSLLDTSLSNVSIPTFVKTLRGCEIVYLACGEAHTVALTRDGGVFTFGAGRYGQLGHNESTIGISVPRKITEFMGNVVTQIACGRFHTLALVPKTGRLFAFGQGANAQLGMGDTTNRLLPC-PIKGHWVSSHKLNSKESTVGTDSSSSKPYVIRRIFSGGDHTFLLAQLYQTGEIVDADDLRNWNRSSLQSILTVCPEIVEQLRSAHTALALPPPISRCISLSSSRPLTSLSSTSNEPMAIVSKVESVYSSLGCLSASCLEEKEPNSHFKTGREEHGVDLDLACTLQSQIFHILPKRDLKRLINKLLDDVLSTSRL--NYPSIECLRGLMFIAVSDLLNMPRKPIIDEAVSDVNKMDITSEMNTNFEPYPVLSAGFSTPGLVLNAYSVAINRLDPAPSKILDRWFTKMQPRYLKKLVLNLNNLISYILDMQPASAYSQRKLKEESIRQALEFMKRLHRVNENHPNPISYNSFYLPSLKDKINIGSAFVNWLQECNSGGMRFFSFCDYPFVFDAASKAEMLNIEARLTMQQAMSQAQQSAIFQSLLSPFIGSRMYDGGTTSPYFTIIVRRDNILQDTLSNLTMANPADFKKLLRVKFENEEAVDEGGVMKEFFLLVMRDLLNPVYGMFRCYSESRMLWFSESTMESENV--FLMVGILCGLAIYNSIIVDLSFPLAMFRKLLGETPGLDDLKELDPIVGRSLQQLLDYDNADLADVFCLNFTVTIDYFGVNKI-IPLMEDGENMIVTQENKSLYVEKYVEYVFQKSCETPYKAFEKGFLQVCGGYSLKLFQPSELQSLVVGSEVVNWDELRQNTTYQGVYWDRHQVIIWFWDVLLYDFTIEEKKKFLRFLTGCDRVPIVGFSGVKITIQPNNSGDDFLPVAHTCANLLDLPQYSCKEILAKKLSLAIQQTEGFGLV 1174 HSP 2 Score: 85.1149 bits (209), Expect = 9.347e-13 Identity = 71/248 (28.63%), Postives = 124/248 (50.00%), Query Frame = 2
Query: 80 IIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG--HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSI-KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTP-SGFLYSWGSNNYGQLGRDLTTSYESLPLMVK 811
+++ +FSFG+ GQLG+ ++ S PQ+I +S + +I G H+ L +G LY+ G+N + +LG + + K +Q ++ + + +++ D + L+ T LS+ SI V++L G IV +ACG HT+ALT++ +F++GA GQLG ++ +S P++I + I G + L P +G L+++G QLG TT+ LP +K
Sbjct: 155 LVLTDTARVFSFGSNLKGQLGLGFSSEQSISIPQQILCLSGLPVRRIVAGGWHSFVLTVSGSLYAWGSNNYGQLGLSYFGSSPKDHQNELTLTYGSVGSTSVDDEQSKDRPFSLLDT------------SLSNVSIPTFVKTLRGCEIVYLACGEAHTVALTRDGGVFTFGAGRYGQLGHNESTIGISVPRKITEFMGNVVTQIACGRFHTLALVPKTGRLFAFGQGANAQLGMGDTTN-RLLPCPIK 389 HSP 3 Score: 80.4925 bits (197), Expect = 2.235e-11 Identity = 59/179 (32.96%), Postives = 88/179 (49.16%), Query Frame = 2
Query: 554 TKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPI-KSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGI 1087
+ E+F+WG+ GQL L N + ++ NLP IT G + LT G +YS G N + QLG + + ++H R I+ V+ G H AA++ +G +FTWG GQLG + + +V L KV Q+ G+ HTL + T V++FG GQLG+
Sbjct: 4 SSTEELFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTFIQITCGYAHTVFLTVDGEVYSCGCNEFQQLGHGQAGGQLAKVVALEHYR---IISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG--DDTGVKLVRALSDHKVVQVSLGVEHTLV-LTDTARVFSFGSNLKGQLGL 176
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Match: A0A5K4EPN5 (Putative hect E3 ubiquitin ligase OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 719.924 bits (1857), Expect = 0.000e+0 Identity = 443/1197 (37.01%), Postives = 655/1197 (54.72%), Query Frame = 2
Query: 65 LNFEFI--IILK----MIS-----IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDM-VSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGR---------------------------------------DLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFE-NKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQS--KISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSIS---------------------------TMKSISEVXXXXXXXXXXXXXDIK----NSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEI---------------------------------SEFCK--VILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---FSLFDYPFLFDGITKTNLMKIEAK-----------------CLMSEGLPCFMAQSS-----FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQ-IIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
LNF FI ++L M+S +F++G+ GQL +D + P+ +D + + +I+CG+ HT+FL +G++YSCG NEF++LGH + + ++ L+ +++I + G HNA +T GL+FTWG NS G+LGR+ D +KLV++L+ H +VQV+ G +HTL LT + +F++G+N GQLGLG + + VS PQQI LS LP++ I AG SFVLT SG LY+WGSNNYGQLG D + S S+P VK LR +VY++ G+ H AL+ DGGVFT+G GRYGQLGHN S+P + + +G+ V+QI CG HTLA + TG ++AFG G QLG+ DT P+ GHWV ++ + + ++++ + YI++R++ GG+H+ + A G+ V PDDLR WN +I + ++ E + +T+++ M +S+V + S ++ NS F + HG+++DL + SQ F L+ L ++ DN+ S + NYPS+ECLR + +VS +L + S F ++L +SV+ + + PS L++W ++PRY KK + N I+ ++ ++ ++ + + +LH++NE+H I YNTFY+ + ++ ++ WL+ + FS DYPF+FD +K ++ IEA+ L+S + M S F I VRRDN+L DT+S L A KK RV F E+A+DEGGVMKE FLLV+ +LNP YG+F YPESR+ WF++ + +N+ F +G L GLAIYN++++DL FPL MF+KLL E LDDL EL+P +G+SLQ LLDYD D D+F LNF V+++ +G L+ DG + VT N+ YV+ YV+Y+ K+ E P+K F++GF G LK F+P ELQ L+VGSE +W LR++ Y G YW+ HQ II ++ + +EKKKFLR+LTGCDRVPI G S +KI IQP N D++LPVAHTC NLLDLP+Y+ + IL KL +AI+ EGF LV
Sbjct: 38 LNFTFITNVLLSKDTLMVSSATEELFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTLIQITCGYTHTVFLTIDGEVYSCGCNEFQQLGHGQSGGQLAKVVALEHYRIISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG-DDTGVKLVRALSDHKVVQVSLGVEHTLVLTDTARVFAFGSNLKGQLGLGYSSEQSVSIPQQILCLSGLPVRRIVAGGWHSFVLTVSGSLYAWGSNNYGQLGLSYFGSSPNDHQNELTLKCGSVGSTSVDDEQSKNRPFSPLDTSLSNVSIPTFVKTLRGCEVVYLACGETHTVALTRDGGVFTFGAGRYGQLGHNESTIGISVPRKITEFMGNVVTQIACGRFHTLALVPKTGRLFAFGQGTNAQLGMGDTKNRLLPC-PVKGHWVSSHKSSSNESTVGTDSSSSKPYIIRRIFSGGDHTYLLAQMYQAGETVDPDDLRNWNRSSLQSILTICPEIVEQLRNAHTALALPPPISRSISLSSSRPLTSSLSSTSNEPMTIVSKVESVFSSLGCLSASCLEEKEPNSHFKTDREEHGVDLDLACKLQSQIFHILPKRDLKRLINKLLDNVLSTSRL--NYPSIECLRGLMFLSVSDLLNMPRKPIMDEAVSDVNNIDITSEKNTNFEPYPVLSSGFSTPGLVLNAYSVAINRLDPAPSKILDRWFTKMQPRYLKKLVLNLNNLISYILDMEPASAYSQRKLKEESIRQALEFMKRLHRVNENHPNPISYNTFYLTSLKDKINIGSAFVNWLQECNSGGMRFFSFCDYPFVFDAASKAEMLNIEARLTMQQAMSQAQQSAIFQSLLSPFIGTRMYDGSTSSPYFTIIVRRDNILQDTLSNLTMANPADFKKLLRVKFENEEAVDEGGVMKEFFLLVMRDLLNPVYGMFRCYPESRMLWFSESTMESENV--FLMVGILCGLAIYNSIIVDLSFPLAMFRKLLGETPGLDDLKELDPIVGRSLQELLDYDNADLADVFCLNFTVTIDYFGMNKIIPLMEDGENIIVTQENKSLYVEKYVEYVFQKSCETPYKAFEKGFLQVCGGYSLKLFQPSELQSLVVGSEVVNWDELRQNTTYQGIYWDRHQVIIWFWDVLLYDFTIEEKKKFLRFLTGCDRVPIVGFSGVKITIQPNNSGDDFLPVAHTCANLLDLPQYSCKEILAKKLSLAIQQTEGFGLV 1228 HSP 2 Score: 89.3521 bits (220), Expect = 4.804e-14 Identity = 66/199 (33.17%), Postives = 97/199 (48.74%), Query Frame = 2
Query: 494 SLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLP-IKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGI 1087
SL FI V D + + E+F+WG+ GQL L N + ++ NLP + IT G + LT G +YS G N + QLG + + + ++H R I+ V+ G H AA++ +G +FTWG GQLG + + +V L KV Q+ G+ HTL + T V+AFG GQLG+
Sbjct: 37 SLNFTFITNVLLSKDTLMVSSATEELFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTLIQITCGYTHTVFLTIDGEVYSCGCNEFQQLGHGQSGGQLAKVVALEHYR---IISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG--DDTGVKLVRALSDHKVVQVSLGVEHTLV-LTDTARVFAFGSNLKGQLGL 229
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Match: G4VNS7 (Putative hect E3 ubiquitin ligase OS=Schistosoma mansoni OX=6183 GN=Smp_142890 PE=4 SV=1) HSP 1 Score: 718.768 bits (1854), Expect = 0.000e+0 Identity = 435/1173 (37.08%), Postives = 644/1173 (54.90%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDM-VSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGR---------------------------------------DLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFE-NKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQS--KISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPI---YTSIS---------------------------TMKSISEVXXXXXXXXXXXXXDIK----NSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEI---------------------------------SEFCK--VILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---FSLFDYPFLFDGITKTNLMKIEAK-----------------CLMSEGLPCFMAQSS-----FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQ-IIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+F++G+ GQL +D + P+ +D + + +I+CG+ HT+FL +G++YSCG NEF++LGH + + ++ L+ +++I + G HNA +T GL+FTWG NS G+LGR+ D +KLV++L+ H +VQV+ G +HTL LT + +F++G+N GQLGLG + + VS PQQI LS LP++ I AG SFVLT SG LY+WGSNNYGQLG D + S S+P VK LR +VY++ G+ H AL+ DGGVFT+G GRYGQLGHN S+P + + +G+ V+QI CG HTLA + TG ++AFG G QLG+ DT P+ GHWV ++ + + ++++ + YI++R++ GG+H+ + A G+ V PDDLR WN +I + ++ E + +T+++ M +S+V + S ++ NS F + HG+++DL + SQ F L+ L ++ DN+ S + NYPS+ECLR + +VS +L + S F ++L +SV+ + + PS L++W ++PRY KK + N I+ ++ ++ ++ + + +LH++NE+H I YNTFY+ + ++ ++ WL+ + FS DYPF+FD +K ++ IEA+ L+S + M S F I VRRDN+L DT+S L A KK RV F E+A+DEGGVMKE FLLV+ +LNP YG+F YPESR+ WF++ + +N+ F +G L GLAIYN++++DL FPL MF+KLL E LDDL EL+P +G+SLQ LLDYD D D+F LNF V+++ +G L+ DG + VT N+ YV+ YV+Y+ K+ E P+K F++GF G LK F+P ELQ L+VGSE +W LR++ Y G YW+ HQ II ++ + +EKKKFLR+LTGCDRVPI G S +KI IQP N D++LPVAHTC NLLDLP+Y+ + IL KL +AI+ EGF LV
Sbjct: 9 LFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTLIQITCGYTHTVFLTIDGEVYSCGCNEFQQLGHGQSGGQLAKVVALEHYRIISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG-DDTGVKLVRALSDHKVVQVSLGVEHTLVLTDTARVFAFGSNLKGQLGLGYSSEQSVSIPQQILCLSGLPVRRIVAGGWHSFVLTVSGSLYAWGSNNYGQLGLSYFGSSPNDHQNELTLKCGSVGSTSVDDEQSKNRPFSPLDTSLSNVSIPTFVKTLRGCEVVYLACGETHTVALTRDGGVFTFGAGRYGQLGHNESTIGISVPRKITEFMGNVVTQIACGRFHTLALVPKTGRLFAFGQGTNAQLGMGDTKNRLLPC-PVKGHWVSSHKSSSNESTVGTDSSSSKPYIIRRIFSGGDHTYLLAQMYQAGETVDPDDLRNWNRSSLQSILTICPEIVEQLRNAHTALALPPPISRSISLSSSRPLTSSLSSTSNEPMTIVSKVESVFSSLGCLSASCLEEKEPNSHFKTDREEHGVDLDLACKLQSQIFHILPKRDLKRLINKLLDNVLSTSRL--NYPSIECLRGLMFLSVSDLLNMPRKPIMDEAVSDVNNIDITSEKNTNFEPYPVLSSGFSTPGLVLNAYSVAINRLDPAPSKILDRWFTKMQPRYLKKLVLNLNNLISYILDMEPASAYSQRKLKEESIRQALEFMKRLHRVNENHPNPISYNTFYLTSLKDKINIGSAFVNWLQECNSGGMRFFSFCDYPFVFDAASKAEMLNIEARLTMQQAMSQAQQSAIFQSLLSPFIGTRMYDGSTSSPYFTIIVRRDNILQDTLSNLTMANPADFKKLLRVKFENEEAVDEGGVMKEFFLLVMRDLLNPVYGMFRCYPESRMLWFSESTMESENV--FLMVGILCGLAIYNSIIVDLSFPLAMFRKLLGETPGLDDLKELDPIVGRSLQELLDYDNADLADVFCLNFTVTIDYFGMNKIIPLMEDGENIIVTQENKSLYVEKYVEYVFQKSCETPYKAFEKGFLQVCGGYSLKLFQPSELQSLVVGSEVVNWDELRQNTTYQGIYWDRHQVIIWFWDVLLYDFTIEEKKKFLRFLTGCDRVPIVGFSGVKITIQPNNSGDDFLPVAHTCANLLDLPQYSCKEILAKKLSLAIQQTEGFGLV 1175 HSP 2 Score: 83.1889 bits (204), Expect = 3.794e-12 Identity = 60/179 (33.52%), Postives = 90/179 (50.28%), Query Frame = 2
Query: 554 TKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLP-IKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGI 1087
+ E+F+WG+ GQL L N + ++ NLP + IT G + LT G +YS G N + QLG + + + ++H R I+ V+ G H AA++ +G +FTWG GQLG + + +V L KV Q+ G+ HTL + T V+AFG GQLG+
Sbjct: 4 SATEELFAWGSCEEGQLTLDKNDAYQILIPKALDVRNLPTLIQITCGYTHTVFLTIDGEVYSCGCNEFQQLGHGQSGGQLAKVVALEHYR---IISVACGAYHNAAITYNGLLFTWGCNSNGQLGREG--DDTGVKLVRALSDHKVVQVSLGVEHTLV-LTDTARVFAFGSNLKGQLGL 176
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Match: A0A4S2LMJ3 (HECT domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006165 PE=4 SV=1) HSP 1 Score: 690.649 bits (1781), Expect = 0.000e+0 Identity = 419/1153 (36.34%), Postives = 614/1153 (53.25%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDM-VSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDL------------TTSYES-------LPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENK-SIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPL-ECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVM-------------NVDVNEPIY-------------TSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISE--------------------------------------FCKVILIP--------FSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE---FSLFDYPFLFDGITKTNLMKIEAKCLMSE-------------------GLPCFMAQSSF-HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIX-XXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+F +GA QLG ++ S PQ I S I +++CG+ HTL L +G++YSCG NEF +LG +I L+ H + D + G HNA ++ G +FTWG NS G+LGR+ D S+K+++SL H +VQV+ G +H+L LT S +F +G+N GQLGLG D+ + PQQ+ LS LPI+ I+AG S VLT SG LY WG N YGQLG + +S ES +P VK L+ Q V+V G++H L+ +GGVFT+G ++GQLGH SIP V DL+ + +Q+ CG HTL + S G VYAFG G GQLG +T P+ G W+ +NQS S + E V RLY GG+H+ ++ +D R+W+ +I + + + P+Y S M + + + + + + ++ F + HG+++D ++ Q F L+ L ++ D + + + NYPS+ECLR ++ AVS +L K+ +P F+++ + + PS +++W ++PRYFKK N I +N ++ S ++ + + +L++INE I Y +FYIP + + +++ S+ WLR FS DYPF+FD TK L+ +EA M G P + S F H+TVRRD L+ DT++ L A L+K RV F GE+A+DEGGVMKE FLLV+ +L+P YG+F YPESR+ WF++ + DN+ F IG L GLAIYN++++DL FPL MF+KLL +K LDDL+EL+P +G+SLQ LLDY+ D + F L F + ++C+G EL+ +G + VT N+ YV+ YVDY+ NK+ E P++ F+ GFH G LKFF+P+ELQ L+VGSE +W LR++ Y G YW++H +I ++ S ++KK+FLR+LTGCDRVP+ G + ++I IQP+N D YLPVAHTC NLLDLP Y+S+ +LR KL IAI++ EGF LV
Sbjct: 9 LFGWGACDDNQLGPHEQDSQQISSPQPITVPSRWTIARVACGYKHTLLLNADGEVYSCGGNEFGQLGRSDAPPDFRRITALENHTITDIACGAYHNAAISYTGRLFTWGCNSNGQLGREG-DDSSVKMIRSLAEHRVVQVSLGLEHSLVLTDTSRLFVFGSNIWGQLGLGFRSDLPIVIPQQLMCLSGLPIRCISAGGVHSAVLTISGTLYVWGGNKYGQLGLEPIEMVAVESVSGSRSSIESAPQHSVSVPTAVKSLKGQRAVFVDCGESHTVVLTEEGGVFTYGGNQFGQLGHGESSKSISIPRKVADLMDNPTTQVACGRMHTLVLVPSLGCVYAFGAGSEGQLGT-NTNTDSSRPMPVKGPWLRSGTSSNQSTNSPDVPLE----VARLYAGGDHAYALTRKSTTKSQIVVEDFRVWDPMSSRSIATVCPQALDHLRDALTALPLPAPMYRTSSTCSESSDSTNSQHVMFASLRLETAFSSLGCLAATCLADNHFSTGRDQHGVDLDAARDLQQQLFRQLQPKNLRRLIEKLMDGVLNVAR--PNYPSIECLRGLMVLAVSDLLNTPRAPDRPNSHDSSHSDISAPIDKHSLSDSDWNESGEAGPFLVKLAGLPNPGMVLHGFTLAINQLEPAPSKIIDRWFARVQPRYFKKLVTNLNNHIVYILNMQPATADYERRSKEEGVRSSLELMKRLYRINESRQPPISYVSFYIPVVREKVNIDSSFLSWLRERESGGMHFFSFCDYPFIFDAPTKARLLSLEATLSMQNAVNQAERNTLIQSVMSPLLGYPVLTSTSPFFHVTVRRDFLIQDTLTYLTVANPSELRKVLRVQFAGEEAVDEGGVMKEFFLLVMRDLLSPIYGMFRCYPESRMLWFSEFTMESDNV--FLLIGILCGLAIYNSIIVDLAFPLAMFRKLLGDKPVLDDLMELDPVVGRSLQQLLDYEGQDVAETFCLTFSLDIDCFGETRHIELVENGSNIEVTHENKSLYVEKYVDYVFNKSCEAPYRAFERGFHQVCAGHALKFFRPMELQSLVVGSEVVNWNELRQNTSYQGVYWDHHPVIEWFWEVLLVEFSVEDKKRFLRFLTGCDRVPLFGFTSLRITIQPMNCGDEYLPVAHTCANLLDLPLYSSKDVLRLKLSIAIQHTEGFGLV 1151
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Match: A0A4Z2CNU8 (Putative E3 ubiquitin-protein ligase HERC4 OS=Schistosoma japonicum OX=6182 GN=EWB00_008797 PE=4 SV=1) HSP 1 Score: 685.641 bits (1768), Expect = 0.000e+0 Identity = 429/1176 (36.48%), Postives = 622/1176 (52.89%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSY--PQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDM-VSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGR------------------------------------DLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFE-NKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLE--CNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWN--------------------------------------WKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILE----------ISEFCK-------------------------VILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLR--NVSKIE-FSLFDYPFLFDGITKTNLMKIEAKCLMSE-------------------GLPCFMAQSS---FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQ-IIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+F +G+ GQL D N D ++ P+ ++ + + +ISCG+ HT+FL +G++YSCG NEF++LGH + + ++ L+ +++I + G HNA VT GL+FTWG NS G+LGR+ D ++KLV++L+ H +VQV+ G +HTL LT + +F++G+N GQLGLG + + V PQQI LS LPI+ ITAG SFVLT SG LY+WGSN YGQLG D S S+P VK LR +VY++ G+ H AL+ DGGVFT+G GRYGQLGHN ++P +++ G+ V+QI CG HTLA + +G ++AFG G GQLG+ DT P+ G WV N QS + + + YI++R++ GG+H+ + A G+KV DDLR WN P T N NEP+ + S + S +CL N F + HG+++DL + + S L+ L ++ DN+ + +N YPS+ECLR + AVS ++ I+E K ++L +SV+ + + PS L++W ++PRY KK + N I+ ++ + ++ ++ + + +LH++NE H I YN FY+P + ++ ++ WL+ N I FS FDYPF+FD +K L+ +EA+ M + G P + +S F I VRR+N+L DT+S L A KK +V F E+A+DEGGVMKE FLLV+ +LNP YG+F YPES + WFN+ + +N+ F G L GLAIYN++++DL FP MF P +G+SLQ LLDYD +D D+F LNF V+++ +G L+ +G + VT N+ YV+ YVDY+ NK+ E +K F++GF G LK F P ELQ L+VGSE +W LR++ Y G YW+ HQ II ++ + +EKKKFLR+LTGCDRVP+ G S +KI IQP N D++LPVAHTC NLLDLP+Y+S+ IL KL +AI+ EGF LV
Sbjct: 8 LFVWGSCEDGQLTSD--NTDTFNVLTPKALNVQNLPEFVQISCGYTHTVFLTIDGEVYSCGCNEFQQLGHRQSGGRLGKVAALEQYRIISVACGAYHNAAVTYSGLLFTWGCNSNGQLGREG-DDTAVKLVRALSDHRVVQVSLGVEHTLVLTDTARVFAFGSNLKGQLGLGFSSEQPVPIPQQILCLSGLPIRRITAGGWHSFVLTVSGSLYAWGSNKYGQLGLSYFGSSPSDHHEFATKYGSSFSVDDEQSKSRSYSPIDTNRSNVSIPTYVKTLRGCEVVYLACGEAHTVALTRDGGVFTFGAGRYGQLGHNELTIGIAVPRKIIEFTGNVVTQIACGRFHTLALIPKSGRLFAFGQGTNGQLGMGDTTNRLLPC-PVKGQWVSCHEFSNRQSVVGNDLQFVKPYIIRRIFSGGDHAYLLAKMYQTGEKVEADDLRNWNRLSLQSIVTICPEIVQQLRNALTALPLPPAVSRSISLSSSRPLTTTSFNPTSNEPMSIVTQVESAFSSLGCLSASCLEEKE---PNCHFKTGREEHGVDLDLAYKLQSSILHVLPQRDLKRLINKLMDNVLTTSHHN--YPSIECLRGLMFLAVSDLMNMPLKPVTDDTITEIKKTDVHLEIIDAVNFYPVLSAGFSTPSLVLNAYSVAVNRLDSAPSKILDRWFTKMQPRYLKKLVLNLNNLISYILDMQPASSYSQRKLKEEGIRQALEFMKRLHRVNESHPNPIPYNRFYLPSLKDKINIGSAFVNWLQECNSGGIRFFSFFDYPFVFDAASKAELLNVEARLSMQQAMTQAQQSAILQTFISPFIGGPVYSGSTSAPYFTIVVRRNNILQDTLSHLTMANPADFKKLLKVQFENEEAVDEGGVMKEFFLLVMRDLLNPVYGMFRCYPESHMLWFNESTMESENV--FLMAGILCGLAIYNSIIVDLSFPQAMFY----------------PIVGRSLQQLLDYDGDDLADVFYLNFSVTIDYFGVNKVIPLMENGENIVVTQENKNLYVEKYVDYVFNKSCETSYKAFEKGFLQVCNGYSLKLFHPFELQALVVGSEVINWDELRQNTTYQGVYWDRHQVIIWFWEVLLHDFTVEEKKKFLRFLTGCDRVPVVGFSGVKITIQPNNSGDDFLPVAHTCANLLDLPQYSSKEILSKKLSLAIQQTEGFGLV 1156 HSP 2 Score: 83.5741 bits (205), Expect = 2.322e-12 Identity = 72/251 (28.69%), Postives = 122/251 (48.61%), Query Frame = 2
Query: 80 IIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWG-----KNSFGELGRDKLSDFSIKL-VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTP-SGFLYSWGSNNYGQLGRDLTTSYESLPLMVK 811
+++ +F+FG+ GQLG+ ++ PQ+I +S I +I+ G H+ L +G LY+ G+N++ +LG P+ DH+ T G F+ S+ + ++ S+ SI V++L G +V +ACG HT+ALT++ +F++GA GQLG + ++ P++I + + I G + L P SG L+++G GQLG TT+ LP VK
Sbjct: 154 LVLTDTARVFAFGSNLKGQLGLGFSSEQPVPIPQQILCLSGLPIRRITAGGWHSFVLTVSGSLYAWGSNKYGQLGLSYFGSSPS-----------------DHHEFATKYGSSFSVDDEQSKSRSYSPIDTNR-SNVSIPTYVKTLRGCEVVYLACGEAHTVALTRDGGVFTFGAGRYGQLGHNELTIGIAVPRKIIEFTGNVVTQIACGRFHTLALIPKSGRLFAFGQGTNGQLGMGDTTN-RLLPCPVK 385
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:NCBI gene;Acc:103028096]) HSP 1 Score: 646.351 bits (1666), Expect = 0.000e+0 Identity = 383/1071 (35.76%), Postives = 581/1071 (54.25%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYP-DDLRLWNWKHPTNITVMNVDVNEPIYT------SISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCK--------LHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIE-----FSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHIT--------------VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P+K + + + CG HT+FLL +G +Y+CG N+ +LGH+ + KP Q+ LDA ++ S G H + + G VF WG+ S G+LG + D +++ ++SL+ IVQV CG+ H+LAL++ +IFSWG N GQLGLG V TPQ +++L +P I+AG SF LT SG ++ WG N +GQLG + S P ++K LR Q +VY+ G++H AAL+ +GGVFT+G G YGQLGHN ++ P V +L+G+ V+QI CG HT+A++ S+G + +FGLG GQLG T + P+ G W I E AE+ Y VKR+Y GG+ S + C + P +DLR+ N ++ +N D+ + S I V S+ S + S G++ ++ + S+ + Q +A ++ N+ + + + P +E LR +L + + IPF+ + + + L W ++P F+ ++ + + + +K +F C LH +NE +II Y+ FYI ++ ++ D+ Y WL+ ++ SL Y F+ D KT L++I+A M + SS +++ VRR+N++GDT+ L ++ KK +V F+GE+A+D GGV KE FLL++ ++L+P YG+F Y ESRL WF F++ + F+ IG + GLAIYN +++L+FP+ ++KKLL LDDL EL P +G+SLQ LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +V+ YVDYI N +V Q F F GFH G L+ F+P ELQ +++G+ +YDW L +S EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM + + IQP ++YLPVAHTC+NLLDLPKY S IL++KL AIE+N+GF L
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVVEPRKCSFFNGKLVWDVGCGRRHTVFLLGDGTVYTCGCNDLGQLGHEKTRKKPEQVVALDAQNIVAVSCGESHTLALNDKGQVFAWGQASEGQLGLANMED-CVRVPRNIKSLSEVHIVQVTCGYRHSLALSRRGQIFSWGQNKYGQLGLGKEGPSVLTPQLVQSLLGIPFAQISAGGAHSFGLTLSGAVFGWGRNKFGQLGLN-DNSDRCAPCLLKTLRSQRVVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNTTNHEINPRKVFELMGNVVTQISCGRQHTMAFIPSSGKIDSFGLGGNGQLGTRSTCNR-KSPAPVKGQWRAY----NDPIPMEEEAEEFYCVKRIYAGGDQSF---AYCQCPQNTSPPEDLRVQNSTQ--HVLTLNSDLIQKWLDHGHGRLSPEITDEIDLVFSSASCLNGSFLSTSHPGHYSTSGKCSGVDENMARVLFSRLIQTDYPHISQQVAASLEKNLIPNL--SSSPPDIEALRLYLTLPQCPLFSNPNTYITLAIPFAKAVTSLKPAALKVLGNWWSRIEPAVFQGMVELYKDVVVYLLR-------MQKVGIPPSEHRLFTCFLSTCLRFLEILHSVNERSGQIIQYDKFYIHELEELVDIRQEYVSWLQQQFSVQPIDSQVSLCRYSFILDAQAKTTLLQIDAVFQMQLAVDQANRLSSMYLSGAAMESVNPCLILIVRRENVVGDTMEVLRKSKNVDYKKPLKVIFVGEEAVDAGGVRKEFFLLIMKELLDPKYGMFRYYEESRLIWFADKTFEE-VDLFHLIGVICGLAIYNLTIVELNFPVALYKKLLKRTPTLDDLKELMPDVGRSLQQLLDYTEDDVEETFCLNFTITVENFGATEVLELVPNGMDINVNNHNRQDFVNAYVDYIFNTSVAQLFNAFHAGFHKVCGGNVLELFQPNELQAMVIGNTNYDWKELEQSAEYKGEYWAGHPTIKLFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLALVIQPTGGGEHYLPVAHTCFNLLDLPKYNSRQILQEKLLKAIEHNQGFNLA 1049
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:NCBI gene;Acc:103028096]) HSP 1 Score: 632.484 bits (1630), Expect = 0.000e+0 Identity = 380/1075 (35.35%), Postives = 576/1075 (53.58%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHW------VPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYT------SISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCK--------LHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKI----EFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHIT--------------VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P+K + + + CG HT+FLL +G +Y+CG N+ +LGH+ + KP Q+ LDA ++ S G H + + G VF WG+ S G+LG + D +++ ++SL+ IVQV CG+ H+LAL++ +IFSWG N GQLGLG V TPQ +++L +P I+AG SF LT SG ++ WG N +GQLG + S P ++K LR Q +VY+ G++H AAL+ +GGVFT+G G YGQLGHN ++ P V +L+G+ V+QI CG HT+A++ S+G + +FGLG GQLG T + P+ G W +P+E E Y R+ G IT DC P+DLR+ N ++ +N D+ + S I V S+ S + S G++ ++ + S+ + Q +A ++ N+ + + + P +E LR +L + + IPF+ + + + L W ++P F+ ++ + + + +K +F C LH +NE +II Y+ FYI ++ ++ D+ Y WL+ + + SL Y F+ D KT L++I+A M + SS +++ VRR+N++GDT+ L ++ KK +V F+GE+A+D GGV KE FLL++ ++L+P YG+F Y ESRL WF F++ + F+ IG + GLAIYN +++L+FP+ ++KKLL LDDL EL P +G+SLQ LLDY ++D E+ F LNF +++E +G EL+ +G D V NRQ +V+ YVDYI N +V Q F F GFH G L+ F+P ELQ +++G+ +YDW L +S EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM + + IQP ++YLPVAHTC+NLLDLPKY S IL++KL AIE+N+GF L
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVVEPRKCSFFNGKLVWDVGCGRRHTVFLLGDGTVYTCGCNDLGQLGHEKTRKKPEQVVALDAQNIVAVSCGESHTLALNDKGQVFAWGQASEGQLGLANMED-CVRVPRNIKSLSEVHIVQVTCGYRHSLALSRRGQIFSWGQNKYGQLGLGKEGPSVLTPQLVQSLLGIPFAQISAGGAHSFGLTLSGAVFGWGRNKFGQLGLN-DNSDRCAPCLLKTLRSQRVVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNTTNHEINPRKVFELMGNVVTQISCGRQHTMAFIPSSGKIDSFGLGGNGQLGTRSTCNR-KSPAPVKGQWRAYNDPIPME-------------EGQYTQHRIMG------IT----DCQNTSPPEDLRVQNSTQ--HVLTLNSDLIQKWLDHGHGRLSPEITDEIDLVFSSASCLNGSFLSTSHPGHYSTSGKCSGVDENMARVLFSRLIQTDYPHISQQVAASLEKNLIPNL--SSSPPDIEALRLYLTLPQCPLFSNPNTYITLAIPFAKAVTSLKPAALKVLGNWWSRIEPAVFQGMVELYKDVVVYLLR-------MQKVGIPPSEHRLFTCFLSTCLRFLEILHSVNERSGQIIQYDKFYIHELEELVDIRQEYVSWLQQQFSVPIDSQVSLCRYSFILDAQAKTTLLQIDAVFQMQLAVDQANRLSSMYLSGAAMESVNPCLILIVRRENVVGDTMEVLRKSKNVDYKKPLKVIFVGEEAVDAGGVRKEFFLLIMKELLDPKYGMFRYYEESRLIWFADKTFEE-VDLFHLIGVICGLAIYNLTIVELNFPVALYKKLLKRTPTLDDLKELMPDVGRSLQQLLDYTEDDVEETFCLNFTITVENFGATEVLELVPNGMDINVNNHNRQDFVNAYVDYIFNTSVAQLFNAFHAGFHKVCGGNVLELFQPNELQAMVIGNTNYDWKELEQSAEYKGEYWAGHPTIKLFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLALVIQPTGGGEHYLPVAHTCFNLLDLPKYNSRQILQEKLLKAIEHNQGFNLA 1037
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:103047299]) HSP 1 Score: 572.007 bits (1473), Expect = 0.000e+0 Identity = 363/1071 (33.89%), Postives = 582/1071 (54.34%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSK---ISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMN--------VDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF---------SLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G++++GQLG+ + + P+ S +++++C H+LFLL +G +YSCG+N +LGH+ KP + LDA K+ + G H+ V G VF WG G+LG + + +L++ L H I QV CG+ H +AL K+ ++F+WG N+ GQLGLG +PQ +K+L +P+ I+AG SF L+ SG ++ WG N+ GQLG + ++P +K LR Q +VY+S G H AAL+ DGG+FT+G+G GQLGH++ N+ +P V++L+G++VSQI CG +HTLA++ STG VYAFG GQLG+ + +PL N S +T+ YI R++ GG+HS + S +K P+D R+ N +I+++N V + + +SI+ + +L S+ +S + F + G++++ + S L+ + + Q+ + P +E +R +LI + L S+ + IP +++ + PS L+ W + F + ++ + + + G + I L KLH++N+ + + YN FYIP I + D++ Y W + ++I+ +L YPF+ + KT +++ +A+ M L +AQ+ F + VRR++L+ D + +L LKK +V F GE+A+D GGV KE FLL++ ++L+P YG+F Y ES L WF+ F ++ FN IG + GLAIYN+ ++DLHFPLV++KKLL+ +LDDL EL+PT G+SLQ LLDY+ +D E+ F LNF ++ E YG EL+ G + V SNR+ +V Y+ Y+ + +V++ + F GF G+ L F+P EL ++VG+ SY+W L K+ Y GE+ +H + FW F ++KK+FL +LTG DR+PI+GM+ ++I +Q + ++YLPVAHTCYNLLD+P Y ++ L +L AIE EGF+LV
Sbjct: 1 MLCWGSSAWGQLGLG-DAEPVVAEPRSCRMFSGRGLKEVACRGQHSLFLLHDGSVYSCGSNSHGQLGHEKPGNKPELVVALDAQKIAGVACGDAHSLAVNEQGQVFAWGTGEKGQLGLGTVEEAVRVPRLIKKLCEHRISQVMCGNQHCIALAKDGQLFTWGQNSSGQLGLGKGEPSSLSPQPLKSLCGIPLAQISAGGDHSFALSLSGAVFGWGRNSAGQLGLN-DEQDRAVPCHIKFLRSQKVVYISCGDEHTAALTKDGGLFTFGNGSRGQLGHDSTNNEPLPRRVLELMGTEVSQIVCGKHHTLAFVPSTGLVYAFGCNAHGQLGLANR----------GNARIPLPVRNIQPPYLSSSQTSDTDHYIPSRIHCGGDHSFLLLSR----EKNCPEDFRIINTS--KSISLINDESLNSWRVRLLDGADSSITN--DVILLLSSTACWNASFLDQSDDGHFKTNPKVPGIDLNSVRLLFETLMKPSYSRLLEQTTKSFESLLIPQLPCSP--PDVEAMRIYLILSECPALHDSKNYISLTIPLAMAILRLDANPSKVLDNWWSLVDCEVFSRLVEMYKSIVVFLLTGGKALLIPVFLESYISA--TLRLLEKLHKVNQKAHHV-EYNRFYIPDITSLVDIQEDYLKWFLSKAEIKMRSPPVQNSVTLCAYPFILNAQAKTTMLQTDAELQMQMAVSGANLHNVFMLLTLEPLLAQNPFLVLHVRRNHLVSDALRELTIYNDVDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLDPVYGMFTHYSESNLLWFSDKCFVEH-NWFNLIGIVCGLAIYNSTVVDLHFPLVLYKKLLDVSPSLDDLKELSPTEGRSLQQLLDYEGDDIEETFCLNFAITREHYGMTEVKELVPGGENITVDRSNREEFVQAYLRYVFSDSVQELYSAFSGGFLKVCGGRILSLFQPSELMAMVVGNSSYNWEELEKNAVYKGEFSASHPTVRMFWEVFHDFPLEKKKQFLLFLTGSDRIPIHGMASLRIVLQSTSAAEHYLPVAHTCYNLLDMPCYKTKETLHLRLTRAIEQYEGFSLV 1045 HSP 2 Score: 148.673 bits (374), Expect = 8.554e-36 Identity = 106/327 (32.42%), Postives = 172/327 (52.60%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGID-PRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG----HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGE--LGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGG-VFTWGHGRYGQLGHNNFENKSIPCIVMDL----LGSK---------VSQIECGMNHT 1018
S++S G+ S+GQLG + P NK P+ + + + I ++CG H+L + + G++++ G E +LG + +V P I+ L H++ G H + G +FTWG+NS G+ LG+ + S S + ++SL G + Q++ G DH+ AL+ + +F WG N+ GQLGL D D + P IK L + + I+ GD+ + LT G L+++G+ + GQLG D +T+ E LP V L + + G++H A G V+ +G +GQLG N N IP V ++ L S S+I CG +H+
Sbjct: 53 SVYSCGSNSHGQLGHEKPGNK-----PELVVALDAQKIAGVACGDAHSLAVNEQGQVFAWGTGEKGQLGLGTVEEAVRV-PRLIKKLCEHRISQVMCGNQHCIALAKDGQLFTWGQNSSGQLGLGKGEPSSLSPQPLKSLCGIPLAQISAGGDHSFALSLSGAVFGWGRNSAGQLGLNDEQDR-AVPCHIKFLRSQKVVYISCGDEHTAALTKDGGLFTFGNGSRGQLGHD-STNNEPLPRRVLELMGTEVSQIVCGKHHTLAFVPSTGLVYAFGCNAHGQLGLANRGNARIPLPVRNIQPPYLSSSQTSDTDHYIPSRIHCGGDHS 371
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:103047299]) HSP 1 Score: 560.451 bits (1443), Expect = 0.000e+0 Identity = 367/1110 (33.06%), Postives = 587/1110 (52.88%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVP------------------------LECNNQSKIS-GETTAEQM-----------------YIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMN--------VDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF---------SLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G++++GQLG+ + + P+ S +++++C H+LFLL +G +YSCG+N +LGH+ KP + LDA K+ + G H+ V G VF WG G+LG + + +L++ L H I QV CG+ H +AL K+ ++F+WG N+ GQLGLG +PQ +K+L +P+ I+AG SF L+ SG ++ WG N+ GQLG + ++P +K LR Q +VY+S G H AAL+ DGG+FT+G+G GQLGH++ N+ +P V++L+G++VSQI CG +HTLA++ STG VYAFG GQLG+ + P+ P L C ++S +AEQM YI R++ GG+HS + S +K P+D R+ N +I+++N V + + +SI+ + +L S+ +S + F + G++++ + S L+ + + Q+ + P +E +R +LI + L S+ + IP +++ + PS L+ W + F + ++ + + + G + I L KLH++N+ + + YN FYIP I + D++ Y W + ++I+ +L YPF+ + KT +++ +A+ M L +AQ+ F + VRR++L+ D + +L LKK +V F GE+A+D GGV KE FLL++ ++L+P YG+F Y ES L WF+ F ++ FN IG + GLAIYN+ ++DLHFPLV++KKLL+ +LDDL EL+PT G+SLQ LLDY+ +D E+ F LNF ++ E YG EL+ G + V SNR+ +V Y+ Y+ + +V++ + F GF G+ L F+P EL ++VG+ SY+W L K+ Y GE+ +H + FW F ++KK+FL +LTG DR+PI+GM+ ++I +Q + ++YLPVAHTCYNLLD+P Y ++ L +L AIE EGF+LV
Sbjct: 1 MLCWGSSAWGQLGLG-DAEPVVAEPRSCRMFSGRGLKEVACRGQHSLFLLHDGSVYSCGSNSHGQLGHEKPGNKPELVVALDAQKIAGVACGDAHSLAVNEQGQVFAWGTGEKGQLGLGTVEEAVRVPRLIKKLCEHRISQVMCGNQHCIALAKDGQLFTWGQNSSGQLGLGKGEPSSLSPQPLKSLCGIPLAQISAGGDHSFALSLSGAVFGWGRNSAGQLGLN-DEQDRAVPCHIKFLRSQKVVYISCGDEHTAALTKDGGLFTFGNGSRGQLGHDSTNNEPLPRRVLELMGTEVSQIVCGKHHTLAFVPSTGLVYAFGCNAHGQLGLANRGN-ARIPLPVRNIQPPYLSSSQTSDRKSRPEDRSSIIRRILACFSRSSARVSPHSAEQMAAIESGDLGNSDSDTDHYIPSRIHCGGDHSFLLLSR----EKNCPEDFRIINTS--KSISLINDESLNSWRVRLLDGADSSITN--DVILLLSSTACWNASFLDQSDDGHFKTNPKVPGIDLNSVRLLFETLMKPSYSRLLEQTTKSFESLLIPQLPCSP--PDVEAMRIYLILSECPALHDSKNYISLTIPLAMAILRLDANPSKVLDNWWSLVDCEVFSRLVEMYKSIVVFLLTGGKALLIPVFLESYISA--TLRLLEKLHKVNQKAHHV-EYNRFYIPDITSLVDIQEDYLKWFLSKAEIKMRSPPVQNSVTLCAYPFILNAQAKTTMLQTDAELQMQMAVSGANLHNVFMLLTLEPLLAQNPFLVLHVRRNHLVSDALRELTIYNDVDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLDPVYGMFTHYSESNLLWFSDKCFVEH-NWFNLIGIVCGLAIYNSTVVDLHFPLVLYKKLLDVSPSLDDLKELSPTEGRSLQQLLDYEGDDIEETFCLNFAITREHYGMTEVKELVPGGENITVDRSNREEFVQAYLRYVFSDSVQELYSAFSGGFLKVCGGRILSLFQPSELMAMVVGNSSYNWEELEKNAVYKGEFSASHPTVRMFWEVFHDFPLEKKKQFLLFLTGSDRIPIHGMASLRIVLQSTSAAEHYLPVAHTCYNLLDMPCYKTKETLHLRLTRAIEQYEGFSLV 1093
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:103047299]) HSP 1 Score: 322.013 bits (824), Expect = 2.873e-93 Identity = 199/555 (35.86%), Postives = 311/555 (56.04%), Query Frame = 2
Query: 1610 PSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWL--------RNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEG--------------LPCFMAQSSFHIT-VRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
P +E +R +LI + L S+ + IP +++ + PS L+ W + F + ++ + + + G + I L KLH++N+ + + YN FYIP I + D++ Y W N++ + +L YPF+ + KT +++ +A+ M L +AQ+ F + VRR++L+ D + +L LKK +V F GE+A+D GGV KE FLL++ ++L+P YG+F Y ES L WF+ F ++ FN IG + GLAIYN+ ++DLHFPLV++KKLL+ +LDDL EL+PT G+SLQ LLDY+ +D E+ F LNF ++ E YG EL+ G + V SNR+ +V Y+ Y+ + +V++ + F GF G+ L F+P EL ++VG+ SY+W L K+ Y GE+ +H + FW F ++KK+FL +LTG DR+PI+GM+ ++I +Q + ++YLPVAHTCYNLLD+P Y ++ L +L AIE EGF+LV
Sbjct: 431 PDVEAMRIYLILSECPALHDSKNYISLTIPLAMAILRLDANPSKVLDNWWSLVDCEVFSRLVEMYKSIVVFLLTGGKALLIPVFLESYISA--TLRLLEKLHKVNQKAHHV-EYNRFYIPDITSLVDIQEDYLKWFLTKLFRLCPNINSV--TLCAYPFILNAQAKTTMLQTDAELQMQMAVSGANLHNVFMLLTLEPLLAQNPFLVLHVRRNHLVSDALRELTIYNDVDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLDPVYGMFTHYSESNLLWFSDKCFVEH-NWFNLIGIVCGLAIYNSTVVDLHFPLVLYKKLLDVSPSLDDLKELSPTEGRSLQQLLDYEGDDIEETFCLNFAITREHYGMTEVKELVPGGENITVDRSNREEFVQAYLRYVFSDSVQELYSAFSGGFLKVCGGRILSLFQPSELMAMVVGNSSYNWEELEKNAVYKGEFSASHPTVRMFWEVFHDFPLEKKKQFLLFLTGSDRIPIHGMASLRIVLQSTSAAEHYLPVAHTCYNLLDMPCYKTKETLHLRLTRAIEQYEGFSLV 979 HSP 2 Score: 261.922 bits (668), Expect = 2.418e-72 Identity = 133/332 (40.06%), Postives = 205/332 (61.75%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS--IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICD 1093
+ +G++++GQLG+ + + P+ S +++++C H+LFLL +G +YSCG+N +LGH+ KP + LDA K+ + G H+ V G VF WG G+LG + + +L++ L H I QV CG+ H +AL K+ ++F+WG N+ GQLGLG +PQ +K+L +P+ I+AG SF L+ SG ++ WG N+ GQLG + ++P +K LR Q +VY+S G H AAL+ DGG+FT+G+G GQLGH++ N+ +P V++L+G++VSQI CG +HTLA++ STG VYAFG GQLG+ +
Sbjct: 1 MLCWGSSAWGQLGLG-DAEPVVAEPRSCRMFSGRGLKEVACRGQHSLFLLHDGSVYSCGSNSHGQLGHEKPGNKPELVVALDAQKIAGVACGDAHSLAVNEQGQVFAWGTGEKGQLGLGTVEEAVRVPRLIKKLCEHRISQVMCGNQHCIALAKDGQLFTWGQNSSGQLGLGKGEPSSLSPQPLKSLCGIPLAQISAGGDHSFALSLSGAVFGWGRNSAGQLGLN-DEQDRAVPCHIKFLRSQKVVYISCGDEHTAALTKDGGLFTFGNGSRGQLGHDSTNNEPLPRRVLELMGTEVSQIVCGKHHTLAFVPSTGLVYAFGCNAHGQLGLAN 330 HSP 3 Score: 147.517 bits (371), Expect = 1.741e-35 Identity = 102/322 (31.68%), Postives = 170/322 (52.80%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGID-PRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLG----HDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGE--LGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGG-VFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTG 1042
S++S G+ S+GQLG + P NK P+ + + + I ++CG H+L + + G++++ G E +LG + +V P I+ L H++ G H + G +FTWG+NS G+ LG+ + S S + ++SL G + Q++ G DH+ AL+ + +F WG N+ GQLGL D D + P IK L + + I+ GD+ + LT G L+++G+ + GQLG D +T+ E LP V L + + G++H A G V+ +G +GQLG N N IP V ++ +S + + + + ST
Sbjct: 53 SVYSCGSNSHGQLGHEKPGNK-----PELVVALDAQKIAGVACGDAHSLAVNEQGQVFAWGTGEKGQLGLGTVEEAVRV-PRLIKKLCEHRISQVMCGNQHCIALAKDGQLFTWGQNSSGQLGLGKGEPSSLSPQPLKSLCGIPLAQISAGGDHSFALSLSGAVFGWGRNSAGQLGLNDEQDR-AVPCHIKFLRSQKVVYISCGDEHTAALTKDGGLFTFGNGSRGQLGHD-STNNEPLPRRVLELMGTEVSQIVCGKHHTLAFVPSTGLVYAFGCNAHGQLGLANRGNARIPLPVRNIQPPYLSSSQTSGHDVILLLSSTA 366
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:ZFIN;Acc:ZDB-GENE-070801-1]) HSP 1 Score: 489.189 bits (1258), Expect = 1.170e-157 Identity = 306/843 (36.30%), Postives = 454/843 (53.86%), Query Frame = 2
Query: 797 PLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKP--INGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNH--------GLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIE--MKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN-------------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLM-------------SEGLPCFMAQSSFHITVRRDNLLGDTISQLHR-ATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDD-NLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
P ++K LR Q +VY+ G++H A L+ +GGVFT+G G YGQLGHN+ ++ P V +L+GS V+QI CG HTLA++ S+G +Y+FGLG GQLG T + P + G WVP + E+ IVKR++ GG+ S A V PDD RL H + V++ + E + S+ + + S+V+ SS I S H GL+++ T ++ S + LQ +A ++ + Q+ + P +E +R +L+ ++ + I F+++ + + TP+ L W L P +F K ++ A+ + I + ++ L LH+ N +I Y+ FYI +I + D+ Y W++ + ++ +L +PF+FD KT L++ +A M S +P + VRRDN++GDT+ L + A KK +V F GEDA+D GGV KE FLL++ +L+P YGLF+ Y ESRL WF++ F+D NL FN IG + GLAIYN+ ++DL+FPL ++KKLL + L+DL EL P +G+SLQ LLDY +D E+ F LNF V+ E +G EL+ G D V +NRQ +V+ YVDYI NK+V+Q F+ F GFH GK L+ F+P EL +++G+ YDW L K+ EY GEYW H I FW F L + KKKFL +LTG DR+PI GM +KI IQP + +LPVAHTC+NLLDLPK++ ++ LR+KL +I + +GF+L
Sbjct: 5 PALLKSLRTQKVVYICCGEDHTAILTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGST---SNRKSPFTVQGAWVP----HNGPCPSNVEKEECCIVKRIFAGGDQSF--AHYFPPEDSVEPDDFRLAE-PH-MQVCVLSGPLLEK-WASLPSGRLPSDVVNEIDMIFSSPSC-INGSFLAYGADEHYRTSGKVSGLDMNATRLAFNKLLQSPCTQVLQQMAASLEKTLIPQL--STAPPDVEAMRLYLVLPEFPLMHDERNFSTLAISFAMAITKLGPTPAKVLENWWSVLTPPFFLKLVELY-KAVVVFLLKLHKMGVPPAEEPIFQASLRSALVVLQILHRAN-TRTSVIQYDKFYIGEICDLVDIRRDYIAWIQQQAYRVDVNSAMAELKEVPVTLCSFPFVFDAQAKTTLLQTDAILQMQVAVDEATRRNFSSMFMPIDPENPCLVLMVRRDNIVGDTLEMLRKQAKNLDFKKPLKVIFYGEDAVDAGGVRKEFFLLIMKDLLDPKYGLFKYYEESRLIWFSQQTFEDSNL--FNLIGLICGLAIYNSTIVDLNFPLALYKKLLKVEPTLEDLKELMPDVGRSLQQLLDYPDDDVEETFCLNFTVAFENFGAMEVKELVPGGSDMVVNNANRQDFVNAYVDYIFNKSVQQLFEAFHTGFHKVCGGKVLQLFQPSELMAMVIGNTDYDWAELEKNTEYKGEYWAGHPTIKSFWEVFHELDLELKKKFLLFLTGSDRIPILGMKSLKIVIQPTGGGEQFLPVAHTCFNLLDLPKFSGKAQLREKLLQSITHYQGFSLA 828 HSP 2 Score: 68.9366 bits (167), Expect = 5.386e-12 Identity = 37/104 (35.58%), Postives = 57/104 (54.81%), Query Frame = 2
Query: 314 NKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLS-DFSIKLVQSLTGHFIVQVACGHDHTLALTKNS-EIFSWGANTCGQLGLGDN 619
+K P ++ L K++ G DH A++T G VFT+G +G+LG + + + + + V L G + Q+ACG HTLA +S I+S+G GQLG G
Sbjct: 1 DKCYPALLKSLRTQKVVYICCGEDHTAILTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGST 104 HSP 3 Score: 60.077 bits (144), Expect = 2.472e-9 Identity = 38/110 (34.55%), Postives = 58/110 (52.73%), Query Frame = 2
Query: 485 LVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTP-SGFLYSWGSNNYGQLGRDLTTSYESLPLMVK 811
L++SL +V + CG DHT LTK +F++GA GQLG V+ P+++ L + I G + + P SG +YS+G GQLG T++ +S P V+
Sbjct: 7 LLKSLRTQKVVYICCGEDHTAILTKEGGVFTFGAGGYGQLGHNSTNHEVN-PRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTSNRKS-PFTVQ 114 HSP 4 Score: 55.4546 bits (132), Expect = 7.946e-8 Identity = 32/110 (29.09%), Postives = 58/110 (52.73%), Query Frame = 2
Query: 638 PQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAA-LSIDGGVFTWGHGRYGQLGHNNFENKSIPCIV 964
P +K+L + I G+ + +LT G ++++G+ YGQLG + +T++E P V L + ++ G+ H A + G ++++G G GQLG + N+ P V
Sbjct: 5 PALLKSLRTQKVVYICCGEDHTAILTKEGGVFTFGAGGYGQLGHN-STNHEVNPRKVFELMGSIVTQIACGRQHTLAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTV 113
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Match: ube3a (ubiquitin protein ligase E3A [Source:ZFIN;Acc:ZDB-GENE-041114-190]) HSP 1 Score: 252.677 bits (644), Expect = 1.428e-70 Identity = 150/404 (37.13%), Postives = 228/404 (56.44%), Query Frame = 2
Query: 2036 EFSLFDYPFLFDGITKTNL---------MKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQ---CHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVS-LECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLK-FFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+FS PF+ +TK NL M E + + L + VRRD+++ D + +L LKK V F GE +DEGGV KE F LVV ++ NPD G+F +RL WFN + N QF IG + GLAIYNN ++D+HFP+V+++KL+ ++ DL + +P L +SL+ LL Y + ED M F +S + +G I +L +G D PVT N++ +VDLY +YILN++VE+ FK F+ GFH LK F+P E++ L+ GS D+ L + EY G + + ++I FW++ D+K+ FL++ TG DR P+ G+ +K+ I D + LP AHTC+N+L LP+Y+S L+++L AI + +GF ++
Sbjct: 467 KFSFMTCPFILSAVTK-NLGLYYDNRIRMYSERRITVLHSLVQGQHLPYLRLKVRRDHVIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEELFNPDIGMFTFDESTRLFWFNPSSLE-NEGQFTLIGIVLGLAIYNNCILDIHFPMVVYRKLMGKRGTFHDLADSHPALYQSLKDLLAY-EGSVEDDIMATFRISYFDMFGTPITQDLKENGEDIPVTNDNKKEFVDLYAEYILNQSVEKQFKAFRRGFHMVTSESPLKALFRPEEVELLVCGSRVLDFHALEDATEYDGGFNKSSRVIRDFWDTVHDFCEDKKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDSDRLPTAHTCFNVLLLPEYSSREKLKERLLKAITHAKGFGML 867
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Match: nedd4a (NEDD4 E3 ubiquitin protein ligase a [Source:ZFIN;Acc:ZDB-GENE-051005-2]) HSP 1 Score: 154.066 bits (388), Expect = 5.999e-40 Identity = 114/368 (30.98%), Postives = 184/368 (50.00%), Query Frame = 2
Query: 2156 FHITVRRDNLLGDTISQLHRATQCHL-KKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFE----------MYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIE-YELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQP------VNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
F + + R ++L D+ ++ + L K + F GE LD GGV +E F L+ ++ NP YGLFE + P S LC +++L F IGR+ G+A+++ ++D F +K +L + L+D+ ++ SL +L+ D D + L F + E +G + EL +G + VT N++ Y++L + + + V++ F EGF + +K F EL+ LM G D R+ Y Y NH +I WFW + + +++ + L+++TG RVP+NG +++ P + LP AHTC+N LDLP Y S LR+KL IAIEN +GF V
Sbjct: 136 FEMKLHRASILEDSYRRIVAVKRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPISGLC------NEEHLSYFTFIGRVAGMAVFHGKLLDGFFIRPFYKMMLQKPITLNDMESVDSEYYNSLMWILENDPADLD----LRFCIDEEMFGQQTHLVELKPNGSEILVTNENKKEYIELVIQWRFVERVQKQINAFMEGFSELIPMDLIKIFDENELELLMCGLGDVDVNDWRQHTIYKNSYCANHPVIQWFWKAVLLMDGEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEMWSTSEKLPRAHTCFNRLDLPPYESFDDLREKLNIAIENAQGFEGV 493
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Match: ube3c (ubiquitin protein ligase E3C [Source:ZFIN;Acc:ZDB-GENE-040426-1211]) HSP 1 Score: 150.599 bits (379), Expect = 3.818e-37 Identity = 111/368 (30.16%), Postives = 175/368 (47.55%), Query Frame = 2
Query: 2153 SFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDE-----GGVMKELFLLVVPQVLNPDYGLFEMY--------PESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLD--DLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSE-SYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDI--KIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFAL 3202
++T+RR + D +L + L++ RV+ + LDE GG+ +E ++ NP G F PE+RL ++ D + +GR+ G A+Y N++++L F KLL ++D L L+P + ++L L Y Q D E++ LNF V G EL G D PVT +NR Y+ L DY LN+ + Q F++G + + L+ F ELQ L+ G++ D L+ Y G Y +H +I FW+ + ++K+K L+++T C R P+ G ++ + D LP A TC NLL LP++ E + KL AIE+ GF L
Sbjct: 730 GINVTIRRPYIYEDAYDKLSPENEPDLRRRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKTGFNPHQGFFRTTNQGLLYPNPEARL-----LVGDGFTRHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTTGDVDIHHLASLDPEVYRNLLFLKSY-QGDVEEL-GLNFTVVNNELGEAQVVELKAGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLATVINLEWLRMFDEQELQVLLSGAQVPIDLQDLKNFTNYSGGYTADHPVIKVFWHVVEGFTDEQKRKLLKFVTSCSRPPLLGFKELFPAFCVHNGGADPERLPTASTCMNLLKLPEFLEEETMHGKLLYAIESAAGFEL 1090
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001275.1 (pep scaffold:Pmarinus_7.0:GL476529:184068:212443:-1 gene:ENSPMAG00000001140.1 transcript:ENSPMAT00000001275.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 142.895 bits (359), Expect = 1.135e-34 Identity = 104/316 (32.91%), Postives = 164/316 (51.90%), Query Frame = 2
Query: 101 SIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFD-HTLFLLKNGKLYSCGNNEFKKLGHDCNKVK--PNQIQ-MLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIK-LVQSLTGHFIVQVACGHDHTLALTKNSEI-FSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENK-SIPCIVMDLLGSKVSQIECGMNHTLAY 1027
S+ + G SYG+LG+ N + + S G D HTL + G+++S G+ ++ KLGH + + P IQ L ++ SAGY H+A VT G ++TWG+ FG LG + +I LV+ ++ + QVACG HT+A++++ I +S+G G+LG GD + V P+ I+ L + I+ + AG + S LT +G LY+WG G T+ P +++ L IV +S G +H AL+ D V+ WG+ GQ G + + + P VM + G V QI G +H+LA+
Sbjct: 430 SVRACGKGSYGRLGLGDSNNQSTLKKLTFEPQRAVKKISSSRGSDGHTLAVTMEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSASVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISN--VGQVACGSSHTIAVSQDGRIVWSFGGGDNGKLGHGDT-NRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQLYAWGCGACLGCGSSEATALR--PKLIEDLACTRIVDISCGDSHCLALTHDNEVYAWGNNSMGQCGQGHSTSPVTKPKKVMGMDGISVQQISAGTSHSLAW 740 HSP 2 Score: 112.079 bits (279), Expect = 3.888e-25 Identity = 72/245 (29.39%), Postives = 129/245 (52.65%), Query Frame = 2
Query: 389 NAVVTNLGLVFTWGKNS---FGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAG---DKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEI 1105
+ VV+ V+ WG NS E ++K+ K+ ++ G T + + + + G + G+LGLGD+ + ST +++ +K I++ D + +T G ++SWG +YG+LG +++ + L+ L+ + +V VS G H A+++ DG ++TWG G +G+LGH + +++IP +V D+ S V Q+ CG +HT+A V++FG G G+LG DT +
Sbjct: 372 SPVVSETSEVYVWGSNSSHQLAEGAQEKI--LQPKMASGFANAQTIEA--GQYCTFVIAVDGSVRACGKGSYGRLGLGDSNNQ-STLKKLTFEPQRAVKKISSSRGSDGHTLAVTMEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSASVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDI--SNVGQVACGSSHTIAVSQDGRIVWSFGGGDNGKLGHGDTNRV 609 HSP 3 Score: 108.997 bits (271), Expect = 3.102e-24 Identity = 78/271 (28.78%), Postives = 132/271 (48.71%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXX-XXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLG-LVFTWGKNSFGELGR-DKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHV---AALSIDGGVFTW 898
+FS+G YG+LG N YP+ I + +S G+ H+ + ++G+LY+ G +F +LGH + + + D + + G H V+ G +V+++G G+LG D + K++++L G FI +V G +LALT ++++WG C LG G + P+ I++L+ I I+ GD LT +Y+WG+N+ GQ G+ +TS + P V + + +S G +H AL D V W
Sbjct: 486 VFSWGDGDYGKLGHG--NSSTQKYPKLIQGPLQGKVVVCVSAGYRHSASVTEDGELYTWGEGDFGRLGHGDSNSRNIPTLVKDISNVGQVACGSSHTIAVSQDGRIVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQLYAWGCGAC--LGCGSSEATALRPKLIEDLACTRIVDISCGDSHCLALTHDNEVYAWGNNSMGQCGQGHSTSPVTKPKKVMGMDGISVQQISAGTSHSLAWTALPTDRQVVAW 752 HSP 4 Score: 92.8189 bits (229), Expect = 2.702e-19 Identity = 74/234 (31.62%), Postives = 123/234 (52.56%), Query Frame = 2
Query: 395 VVTNLGLVFTWGKNSFGELG-RDKLSDFSIKLVQSLTGHFIVQVAC--GHD-HTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKS-----ITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGG-VFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLG 1066
V+ G V GK S+G LG D + ++K + + +++ G D HTLA+T E+FSWG G+LG G+ S+ Q+ L P++ ++AG + S +T G LY+WG ++G+LG + S ++P +VK + + V+ G +H A+S DG V+++G G G+LGH + P ++ L G + ++ G +LA + STG +YA+G G
Sbjct: 424 VIAVDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEPQRAVKKISSSRGSDGHTLAVTMEGEVFSWGDGDYGKLGHGN-----SSTQKYPKLIQGPLQGKVVVCVSAGYRHSASVTEDGELYTWGEGDFGRLGHGDSNS-RNIPTLVKDISN--VGQVACGSSHTIAVSQDGRIVWSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLA-LTSTGQLYAWGCG 648
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Match: HUL4 (Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex [Source:SGD;Acc:S000003797]) HSP 1 Score: 182.185 bits (461), Expect = 1.926e-47 Identity = 138/430 (32.09%), Postives = 221/430 (51.40%), Query Frame = 2
Query: 2006 ELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMS-EGLPCFM--------AQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWF--NKMIFDDN------LPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVS-------LECYGNKIEY---ELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFT-MQGKCLKFFKPIELQELMVGSES---YDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNV-DDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFA 3199
E W + +K FS YPF+ K ++M+ E + +M E F+ F I VRRD + D++ + + Q L KS R+ F+ E +D GG+ KE F L+ + NP GLF ES WF + FD + L + G + GLAI+N+ ++DL FP ++KKL +E + +D EL P ++L +L+Y +++FED+F L F + L + EY EL +G + P+T SN+ +V +V++ L K++E + F GF + +K F EL+ L+ G E +D+ LR +Y+G + ++ + + WFW S + +KK L+++T DR+P G+S I KI + D + LP+AHTC+N + L Y+S+ L KL AI +EG+
Sbjct: 462 EEWGNSTTKKCFSFCKYPFILSLGIKISIMEYEIRRIMEHEAEQAFLISLDKGKSVDVYFKIKVRRDVISHDSLRCI-KEHQGDLLKSLRIEFVNEPGIDAGGLRKEWFFLLTKSLFNPMNGLFIYIKESSRSWFAIDPPNFDKSKGKNSQLELYYLFGVVMGLAIFNSTILDLQFPKALYKKLCSEPLSFEDYSELFPETSRNLIKMLNYTEDNFEDVFSLTFETTYRNNNWILNDSKSSKEYVTVELCENGRNVPITQSNKHEFVMKWVEFYLEKSIEPQYNKFVSGFKRVFAECNSIKLFNSEELERLVCGDEEQTKFDFKSLRSVTKYVGGFSDDSRAVCWFWEIIESWDYPLQKKLLQFVTASDRIPATGISTIPFKISLLGSHDSDDLPLAHTCFNEICLWNYSSKKKLELKLLWAINESEGYG 890
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Match: RSP5 (NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome [Source:SGD;Acc:S000000927]) HSP 1 Score: 158.688 bits (400), Expect = 3.957e-40 Identity = 109/356 (30.62%), Postives = 178/356 (50.00%), Query Frame = 2
Query: 2159 HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWF---NKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNV------DDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFA 3199
HI VRR N+ D ++ R T LKK + F GE+ LD GGV +E F L+ ++ NP Y LFE N I ++L F IGR+ GL +++ +D F ++K +L +K L D+ ++ + SL +L +N + + L F E +G + +L DG + VT N++ YV+LY + + V++ FK F +GF+ + + F EL+ L+ G D +K +Y G Y + ++I WFW S +++ + L++ TG R+P+NG D++ P + LP +HTC+N +DLP+Y ++ KL +A+E GF
Sbjct: 456 HIKVRRKNIFEDAYQEIMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRKKVVLQDMEGVDAEVYNSLNWML---ENSIDGVLDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRG-YQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQLPKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFG 807
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Match: TOM1 (E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase [Source:SGD;Acc:S000002865]) HSP 1 Score: 153.295 bits (386), Expect = 9.304e-38 Identity = 115/360 (31.94%), Postives = 175/360 (48.61%), Query Frame = 2
Query: 2162 ITVRRDNLLGDTISQLHRATQCHLKKS-FRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWF---NKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDI-------KIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAI-ENNEGFALV 3205
ITVRR+ + D+ L T +K S + F GE +D GGV +E + ++ Q+ NPDY LF P + + I ++L F IG + G AI + +D HF ++K +L +L D+ L+P KSL +L +ND DI F V + YG LI G D VT +N+Q YV V+Y L +V++ F GF+ + + F EL+ L+ G D + + Y+ Y + +++FW + S +E+ K L+++TG +VP+NG ++ K I LP +HTC+N L+LP Y S LR L +AI E +EGF L
Sbjct: 2913 ITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWIL---ENDITDIIEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVN-YTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFGLA 3268
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Match: HUL5 (Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock [Source:SGD;Acc:S000003109]) HSP 1 Score: 113.235 bits (282), Expect = 7.866e-26 Identity = 108/358 (30.17%), Postives = 167/358 (46.65%), Query Frame = 2
Query: 2168 VRRDNLLGDTISQLHRATQCHLKKSFRVNFM---GEDA-LDEGGVMKELFLLVVPQVL-NPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEK----ANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGS-ESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMS--DIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFAL 3202
+ RDN+L D + + + K S V F+ GE+A +D GG+ KE V + +P + LF R + +++D L +G++ G +Y +V+ID+ F KKLLN ++ DL + L +L LL+ ++ + + + I E ++ LI +G VT N YV DY LNK +P F G + ++ F IELQ L+ G ++ D L+ + EY G Y Q I FW + F+EK FL+++T + P+ G D K I+ + LP A TC NLL LP Y +++ILR+KL AI + F L
Sbjct: 558 ISRDNVLEDAFNAFNSIGE-RFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRT--NDRYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLSSFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEIDEPESSAKVVD--LIPNGSKTYVTKDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDNIDLDDLKSNTEY-GGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKALDPKFGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARFDL 909
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Match: SRM1 (Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant [Source:SGD;Acc:S000003065]) HSP 1 Score: 101.293 bits (251), Expect = 9.578e-23 Identity = 68/256 (26.56%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 248 LLKNGKLYSCGNNEFKK--LGHDCNKVK----PNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLV--QSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFD---MVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLG-----------RDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKS 949
L NG++Y+ G + LG +K+K P ++ + ++ + G DH + G+VF WG +LGR + F +K + + + +A G +H ALTK++++ SWG N GQ G+ ++ + +V+ P+++ N+ I+SI AG+ S +L+ G LYS G + ++G +D+ ++PL K V+ G +H A++ +G ++WG G +G FE+ +
Sbjct: 180 LFSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTFSKYNIVQLAPGKDHILFLDEEGMVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHVKYIASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRSIAAGEHHSLILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNNVPKFKSVAAGSHHSVAVAQNGIAYSWGFGETYAVGLGPFEDDT 435 HSP 2 Score: 87.4261 bits (215), Expect = 2.897e-18 Identity = 80/342 (23.39%), Postives = 138/342 (40.35%), Query Frame = 2
Query: 347 DAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRD-------------------------KLSDFSIKLVQS-----LTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLG---DNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLG-HNNFENKSI---PCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHW----VPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITA 1249
D K+I + G H + V++WG N G LGRD +L K+ + GH +VQ+A + + AL N E+++WG C + LG D + TP ++ S I + G L G +++WG+ QLGR + + L + + + Y++ G+NH AL+ D + +WG ++GQ G + E+ ++ P + + I G +H+L + G +Y+ G ++GI +T K ++G +P + NN K K + G +HS+ A
Sbjct: 82 DEAKIISFAVGGMHTLALDEESNVWSWGCNDVGALGRDTSNAKEQLKDMDADDSSDDEDGDLNELESTPAKIPRESFPPLAEGHKVVQLAATDNMSCALFSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTFSKYNIVQLAPGKDHILFLDEEGMVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHVKYIASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRSIAAGEHHSL-ILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNNVPKF------------KSVAAGSHHSVAVA 410 HSP 3 Score: 57.3806 bits (137), Expect = 8.463e-9 Identity = 62/246 (25.20%), Postives = 110/246 (44.72%), Query Frame = 2
Query: 227 GFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAH-------KLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG-RDKLSDFSI----KLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGL-GDNF------DMVSTPQQIK---NLSNLP-IKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQP--IVYVSVGQNHVAALSIDGGV 889
G DH LFL + G +++ GN + +LG ++ +++ LD K I ++G +H +T + +WG N FG+ G + + D ++ K + I +A G H+L L+++ +++S G ++G+ DN D+ + + L+N+P KS+ AG S + +G YSWG +G +P +K+ Q I+ V G S+ GGV
Sbjct: 229 GKDHILFLDEEGMVFAWGNGQQNQLGRKV--MERFRLKTLDPRPFGLRHVKYI--ASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRSIAAGEHHSLILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNNVPKFKSVAAGSHHSVAVAQNGIAYSWGFGETYAVGLGPFEDDTEVPTRIKNTATQDHNIILVGCG----GQFSVSGGV 466
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Match: EDO34592 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNX9]) HSP 1 Score: 620.542 bits (1599), Expect = 0.000e+0 Identity = 393/1077 (36.49%), Postives = 588/1077 (54.60%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXX-XXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELG-----RDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQM-YIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWK----HPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLC-----KLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRN----------VSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGL---------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDD--NYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+F +G S GQLG+ +++ S P+ ++ + SS++ ++SCG++HT FL K+G +Y+CG+NEF +LG + + P + ++ + + G H V++ GLVF WG NS+G++G ++K+ K+++ L +VQ+ACG +H++ALT + +F WG N+ GQLG+G +S P + +L+ +P I+AG SF L+ SG L+ WG N YGQLG TT LP ++K LR Q + YVS G+ H A L+ DGGVFT+G G GQLGHN+ ++ P V +L+GS V+QI CG HTLA + S+G VY FG G GQLG+ T C++ G W L NN + E + + Y V+R+ GG+H ASS + P+D+RL H + M++ I + +S + E++FSSLACL+ SFLD N V H I L + F S + + V N + P +E LR +L+ + I+ E ++IPF+ S + L +W L YF + VF + + F++ E + V+ +C LH++N + I+ + F P+ +V D+ Y W++N V K FS +PF+FD KT L++ +A M + P I VRRD+++ DT++Q+ + LKK +V F E+A+D GGV KE FLL++ ++L+P YG+F +S+ WFN + F+++ P F IG + GLAIYN+ + DL FP V++KKLL +K NLDD P++ ++LQ LLDY +D ED F LNF + E YG +L+ G + PVT NR+ YV+LY+DY L+K V++ F+ F GFH G L+FF P EL E++VG + YD+ +L K EY GEY+ H +I FW F KKKFL +LTG DRVPI GM +K+ IQPV + ++LPVAHTC+NLLDLP+Y + ++R KL A+E ++GF L
Sbjct: 1 MFCWGKCSEGQLGVGETEQEFISTPRLLENLEFSSNVREVSCGWNHTAFLCKDGTVYTCGSNEFGQLGQEKSHTNPESVHAIETFTITKVTCGKAHTVAVSDQGLVFAWGDNSYGQIGLGSKVKEKVQTIP-KMLKGLAKMSVVQIACGCNHSMALTISGHLFCWGDNSSGQLGIGKTHSSMSEPAPVLSLNGVPFMLISAGGAHSFALSSSGALFGWGKNEYGQLGVHDTTD-RPLPTLLKSLRSQGVCYVSCGEKHTAVLTSDGGVFTFGCGTSGQLGHNSNHHEYTPRKVFELMGSVVTQIACGRAHTLALVPSSGRVYTFGRGGDGQLGLGVTNN-CNSPMTAKGPW--LRSNNIKSMLPEADGDSVVYTVRRIDAGGDHCFAMASSYG-DETDGPEDMRLPRPSQSILHLSVPVTMDLVKRATIASDLSDVSEKLEIIFSSLACLNGSFLD--NVVHPYVMPIHPCLIPLYVH----FIHSHLTPLPPSQIVSCISNFLLPAMVQQQPPDIEALRVYLMLPENGIMLDKECFMEVVIPFADSILRLSPEAQKVLRRWWSTLPSTYFSRVIQVFQECVKYLLT-----LPLPHFAN--EERQVYLGMCMVALDNLHKVNNESGDIVPFKQFTFPRTREV-DIRADYFNWIQNQKINLKHQHSVVKNTFSFCRFPFVFDASAKTKLLQTDAVMQMQSAVDEVNRRNVQSLLSFVPVNPVNPCLVIKVRRDSIVQDTLTQITTQSPYDLKKPLKVIFANEEAVDAGGVKKEFFLLLLTEILDPKYGMFTYLEDSQTIWFNDLTFEES-PMFLLIGVICGLAIYNSTIFDLRFPPVLYKKLLRKKPNLDDFRGFQPSVARNLQYLLDYSDDDLEDTFGLNFQIMREKYGALEYIDLVPGGGEVPVTQDNREQYVELYIDYYLSKAVDKQFEAFFTGFHRVCGGHVLEFFHPQELMEMVVGCQEYDFEVLEKITEYKGEYYRKHPVIANFWTVFYEFPLSMKKKFLAFLTGSDRVPILGMESMKMIIQPVAGGEHGDHLPVAHTCFNLLDLPRYPTLEMMRTKLSQAVEFSKGFGLA 1056
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Match: EDO40462 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S732]) HSP 1 Score: 259.61 bits (662), Expect = 3.847e-73 Identity = 142/354 (40.11%), Postives = 213/354 (60.17%), Query Frame = 2
Query: 2156 FHITVRRDNLLGDTISQLHRATQ---CHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLK-FFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ VRRD+L+ D + L Q LKK V F GE +DEGGV KE F L+V Q+ NPDYG+F +R+CWFN F+ + QF IG + GLAIYNN+++D+HFP+V+++KL + ++DL + +P L SL LL Y+ N ED+ I + +G+ + +EL +G PVT NRQ YV+L+ D+ILNK+VE+ F F+ GF + LK FF+P E++ L+ GS+ +D+ L K+ EY G + +I WFW S ++K++ L + TG DR+P+ G++ +K+ I D LP AHTC+N+L LP Y ++ L ++L AI + +GF L+
Sbjct: 445 LRLKVRRDHLIEDALVSLEMVAQDNPLDLKKQLFVEFEGEQGIDEGGVSKEFFQLIVEQIFNPDYGMFTYDEATRICWFNPTSFESD-AQFCLIGLVLGLAIYNNIILDIHFPMVVYRKLFGKPGTVEDLKDSHPVLYMSLTELLTYEGNVEEDLMCTFKIGYTDVFGSDLTHELKANGESIPVTKENRQEYVNLHADFILNKSVEKQFTAFKRGFDMVVDESPLKIFFRPDEVEMLVCGSKDFDFDALEKATEYDGGLTKDSALIRWFWEIVHSFDMEQKRQLLMFTTGSDRIPVGGLAKLKLIIAKNGPDSPRLPTAHTCFNVLLLPDYATKEKLEERLLKAITHAKGFGLL 797
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Match: EDO40302 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7U7]) HSP 1 Score: 254.603 bits (649), Expect = 3.508e-72 Identity = 171/518 (33.01%), Postives = 266/518 (51.35%), Query Frame = 2
Query: 1781 FKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRI-IHYNTFYIPKIPKVFDLEYSYELW--LRNVSKI--------EFSLFDYPFLFDGITKTNLMKIEAKCLM------------------------------SEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMG--EDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
F + VF + + ++ Q+++ +I ++ +F K+ Q + ++ + FY ++ K D + Y W R +SK+ + D+PFL D +K +M I+A M ++G+ M + VRRD+L+ DT+SQ+H + LKK ++ ++G E LD GG+ KE F ++V + P YG+F ESR W N F+ +L +F +G + GLAIYN +++D+HFP V++KKL + +L+DLI + P+LG SL+ LL+Y+ D ED F F VS YG + ELI +G + PV NR+ +V YV Y+L +V + F F GFH G L F EL+ L+ G D+ L + Y Y H I FWN F+ ++ +KK L ++TG DRVP+ G+S++ IQ D + LP A TC+N L LP+YTS L+++L +AIEN++GF L
Sbjct: 186 FSRLTRVFCGFLGSHVH--PSQSSESHLMEICKVIGIFFDAYKVIQGSSRPDKTSFQLSVFYCDELSKKLDFKKEYGNWQMARRLSKLPGYSTDKDSLCILDFPFLLDPASKVKVMHIDAVWQMRVEYQNAIVHQARVQQVQRIFEETEKSKKLNDGVKAAMC-PFLVLEVRRDHLIRDTLSQIH-LKEYDLKKPLKIKYVGGGELGLDMGGLQKEFFHMIVDSIFEPGYGMFTYIEESRTMWINGESFE-SLKEFELVGIVLGLAIYNGIILDIHFPQVIYKKLQGQTPSLEDLIGVQPSLGNSLKQLLEYE-GDVEDTFCYTFQVSAMSYGKVCDRELIPNGAEVPVNNENREEFVHRYVKYLLVDSVAKQFDAFSAGFHKVCGGSALTLFCADELELLICGHHVLDFSDLVLAASYDDGYSAQHSTIKMFWNVFNEMTNHQKKALLMFVTGSDRVPLKGLSNLTFIIQKHGQDSDRLPTAMTCFNRLLLPEYTSPKRLKERLIVAIENSKGFGLT 697
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Match: EDO34126 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ93]) HSP 1 Score: 235.728 bits (600), Expect = 2.322e-66 Identity = 157/440 (35.68%), Postives = 235/440 (53.41%), Query Frame = 2
Query: 1988 DLEYSYELWLR-NVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSF-----------------HITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKA---------------NLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNV--DDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFAL 3202
DL Y W + +FS YPF+ K +M+ +++ M MA+ S +I VRR +L+ D+++++ R + +LKK +V F GE LD GG+ KE FLL+V ++ P+YG+F +SRL WFNK D + +FN +G L GLA+YN++++D+HFP +KKLL+ L+DL E+ P L + L+ LL Y + D E+ F VS YG + + L G PVT +NRQ YV LYVDY+LN ++ + F+ F GFH L +P E++ L+ G+ D L+K Y G Y N I +FW++ + + D KKK L + TG DR+PI GM++++ KI ++ + LP+AHTC+N L LP Y + ++ KL IAI N EGF +
Sbjct: 181 DLMAEYYSWQNPGANSGKFSFCQYPFILSLGAKRYIMQKDSESQM-----IVMARKSLMEKVQRREMPDMGMLFLNIKVRRSHLITDSLNEIARKQK-NLKKKLKVTFAGEPGLDMGGLTKEWFLLLVRKIFKPEYGMFSCNDKSRLYWFNKECLDQD-EEFNLVGVLMGLAVYNSIILDIHFPSCCYKKLLSPAVVPFHNPNARVGLANLGLEDLAEVMPELARGLKELLSY-EGDVENDLCQTFQVSFTSYGEVVTHNLKPKGDTIPVTNTNRQEYVQLYVDYLLNSSIYKQFEAFYHGFHSVCASNALIMLRPEEVEMLVCGNPELDMEALKKVTVYDG-YSKNDNTIRYFWDTVMNFNTDLKKKMLLFATGSDRIPIGGMAEMEFKIVRMDTAHSTSMLPMAHTCFNQLCLPPYKTRKQIKQKLTIAISNAEGFGI 611
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Match: EDO49327 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHD5]) HSP 1 Score: 164.466 bits (415), Expect = 2.312e-44 Identity = 116/372 (31.18%), Postives = 192/372 (51.61%), Query Frame = 2
Query: 2117 MSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCW---FNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFM-LNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDI-------KIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFA 3199
M+ LP S ITV R NL ++ Q+ R L++ + F GE+ LD GGV +E F L+ +VLNP Y LFE ++ + D+L F IGR +A+Y+ ID F L +K++LN+K + DL ++P SL + +N+ E+ + + F V +E G ++L G D VT N++ Y+ L ++ LN+ +E+ + F EG H + +++F EL+ ++ G + D +++ Y Y N + + WFW + + +++ + L+++TG R+P+ G +++ K I+ V + +LP +HTC+N LDLP Y S L +KL AIE EGFA
Sbjct: 1 MANTLP-----SHIKITVSRTNLFEESFQQVMRYQPHDLRRRLYITFKGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYANKNNYSLQINAASSVNPDHLMYFKFIGRFIAMALYHGKFIDRGFTLPFYKRMLNKKLLMKDLETIDPEFYNSLVWV---KENNIEECGLEMFFTVDMELLGKVTSHDLKPGGTDIAVTEENKEEYISLMTEWRLNRGIEEQTRAFLEGMHEVLPLYWIQYFDERELELMLCGMQEIDVEDWQQNTVYR-HYTRNSKQVMWFWQAVKAYDNEKRIRLLQFVTGTCRLPVGGFTELMGDNGPQKFCIEKVG-KETWLPRSHTCFNRLDLPPYKSYEQLVEKLTFAIEETEGFA 362
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:NCBI gene;Acc:101174618]) HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0 Identity = 390/1081 (36.08%), Postives = 594/1081 (54.95%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPL---------ECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPT-NITVMNVDVNEPI-YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCK--------LHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWL-RNVSKIE----FSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P+K + + + CG HT FLL++G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G VF+WG S G+LG + + I++ ++SL+ + QVACG+ H+LAL+K IFSWG N GQLGLG +S PQ +++L +P I AG SF LT SG ++ WG N +GQLG + T+ P ++K LR Q ++Y+S G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+G+ V+QI CG HTLA+ S+G + +FGLG GQLG T + P+ G W E + SG E +V R+Y GG+ S SS + + D ++ + + T N V+ +N+P + K I + S+ S F S G++++ ++ + + + + +A ++ N+ ++ + + P +E LR +L S + I IPF+ S + E P L W L+P F++ ++ + + + K +E + +F C LH++NE +II Y+ FYI ++ ++ D+ + Y W+ R + + +L YPF+FD KT L++ +A M + PC + + VRR+N++GDT+ L ++ KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F + +SRL WF+ F+D + FN IG + GLAIYN +++LHFPL ++KKLL +K LDDL EL P +G+SLQ LLDY ++D E+ F LNF ++ E YG +EL+ +G + V NRQ +V+ YVDY+ N +V F+ F GFH GK L+ F+P ELQ +++G+ +YDW L KS EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQP + +LPVAHTC+NLLDLPKYTS LR+KL AI++N GF L
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVVEPRKCEFFHGKRVRDVGCGRRHTAFLLEDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQIIVALSCGESHTLALNDKGQVFSWGLGSDGQLGLNNFEE-CIRVPRNIKSLSDVQVAQVACGYWHSLALSKGGHIFSWGQNRYGQLGLGMTGQSISVPQHLQSLQGIPFAQIAAGGAHSFALTLSGAVFGWGRNKFGQLGLN-DTNDRCFPALLKALRSQRVIYISCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGNVVTQIACGRQHTLAFTPSSGKMDSFGLGGNGQLGTRSTCNR-KSPAPVKGPWASSSASSDSDAGEAADTGAPSGPDIGESC-VVNRIYAGGDQSFAHYSSSSNEQMMESDRIQGLHRRICTVNEDVLQTWLNQPAGRLPLEMSKEIDLIFSSASCINGSFLSSSHPDHFSTSSKCSGVDMNTARLLLHKVTQKEHREISEQVAASLEKNLIPRL--SPSPPDIEALRLYLTLPESPLFWDRNNFVTITIPFAKSVVSLKEAPLKVLGNWWSTLEPPVFQRLVDLYRDVVLYLLQ------MHKVGIPSVESR-IFTCFLDTSLRLLELLHKVNERAGQIIRYDKFYIHELDELIDIRHDYVTWIQRQMYPLGHDGVVTLCKYPFVFDAQAKTTLLQTDAVIQMQMAVDQAQLQNFSSMFMPAVESVNPCLI------LIVRRENIVGDTMEVLRKSKNVDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMKELLDPKYGMFRYHEDSRLIWFSNKTFED-IDLFNLIGLICGLAIYNLTIVELHFPLALYKKLLKKKPTLDDLKELMPDVGRSLQQLLDYTEDDVEETFCLNFTITEENYGATEVHELVPNGEELSVNKGNRQDFVNAYVDYVFNSSVASLFECFYSGFHKVCGGKVLELFQPSELQAMVIGNTNYDWTELEKSTEYKGEYWAEHPTIKLFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYTSPEALREKLLQAIDHNRGFNLA 1061
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Match: herc4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4 [Source:NCBI gene;Acc:101174618]) HSP 1 Score: 639.802 bits (1649), Expect = 0.000e+0 Identity = 390/1072 (36.38%), Postives = 596/1072 (55.60%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKL---VQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPT-NITVMNVDVNEPI-YTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCK--------LHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWL-RNVSKIE----FSLFDYPFLFDGITKTNLMKIEAKCLMSEGL--------------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G ASYGQLG+ +++ P+K + + + CG HT FLL++G +Y+CG N+ +LGH+ ++ KP Q+ LDA ++ S G H + + G VF+WG S G+LG + + I++ ++SL+ + QVACG+ H+LAL+K IFSWG N GQLGLG +S PQ +++L +P I AG SF LT SG ++ WG N +GQLG + T+ P ++K LR Q ++Y+S G++H AAL+ +GGVFT+G G YGQLGHN+ ++ P V +L+G+ V+QI CG HTLA+ S+G + +FGLG GQLG T + P+ G W ++ S I GE+ +V R+Y GG+ S SS + + D ++ + + T N V+ +N+P + K I + S+ S F S G++++ ++ + + + + +A ++ N+ ++ + + P +E LR +L S + I IPF+ S + E P L W L+P F++ ++ + + + K +E + +F C LH++NE +II Y+ FYI ++ ++ D+ + Y W+ R + + +L YPF+FD KT L++ +A M + PC + + VRR+N++GDT+ L ++ KK +V F+GEDA+D GGV KE FLL++ ++L+P YG+F + +SRL WF+ F+D + FN IG + GLAIYN +++LHFPL ++KKLL +K LDDL EL P +G+SLQ LLDY ++D E+ F LNF ++ E YG +EL+ +G + V NRQ +V+ YVDY+ N +V F+ F GFH GK L+ F+P ELQ +++G+ +YDW L KS EY GEYW H I FW F L ++KK+FL +LTG DR+PI GM +K+ IQP + +LPVAHTC+NLLDLPKYTS LR+KL AI++N GF L
Sbjct: 1 MLCWGNASYGQLGLGGIDEEIVVEPRKCEFFHGKRVRDVGCGRRHTAFLLEDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQIIVALSCGESHTLALNDKGQVFSWGLGSDGQLGLNNFEE-CIRVPRNIKSLSDVQVAQVACGYWHSLALSKGGHIFSWGQNRYGQLGLGMTGQSISVPQHLQSLQGIPFAQIAAGGAHSFALTLSGAVFGWGRNKFGQLGLN-DTNDRCFPALLKALRSQRVIYISCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTNHEVNPRKVFELMGNVVTQIACGRQHTLAFTPSSGKMDSFGLGGNGQLGTRSTCNR-KSPAPVKGPWASSSASSDSDI-GESC-----VVNRIYAGGDQSFAHYSSSSNEQMMESDRIQGLHRRICTVNEDVLQTWLNQPAGRLPLEMSKEIDLIFSSASCINGSFLSSSHPDHFSTSSKCSGVDMNTARLLLHKVTQKEHREISEQVAASLEKNLIPRL--SPSPPDIEALRLYLTLPESPLFWDRNNFVTITIPFAKSVVSLKEAPLKVLGNWWSTLEPPVFQRLVDLYRDVVLYLLQ------MHKVGIPSVESR-IFTCFLDTSLRLLELLHKVNERAGQIIRYDKFYIHELDELIDIRHDYVTWIQRQMYPLGHDGVVTLCKYPFVFDAQAKTTLLQTDAVIQMQMAVDQAQLQNFSSMFMPAVESVNPCLI------LIVRRENIVGDTMEVLRKSKNVDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMKELLDPKYGMFRYHEDSRLIWFSNKTFED-IDLFNLIGLICGLAIYNLTIVELHFPLALYKKLLKKKPTLDDLKELMPDVGRSLQQLLDYTEDDVEETFCLNFTITEENYGATEVHELVPNGEELSVNKGNRQDFVNAYVDYVFNSSVASLFECFYSGFHKVCGGKVLELFQPSELQAMVIGNTNYDWTELEKSTEYKGEYWAEHPTIKLFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQPTGGGEQFLPVAHTCFNLLDLPKYTSPEALREKLLQAIDHNRGFNLA 1047
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:101155493]) HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0 Identity = 364/1069 (34.05%), Postives = 575/1069 (53.79%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIK---LVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKH---PTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQI--QYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF------------SLFDYPFLFDGITKTNLMKIEAKCLMSEGL---------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
+ +G A GQLGI + P+ S +++++CG HTLFLL++G +Y+CG+N +LGHD P + LD K+ S G H+ V G VF WG G+LG + ++ +++ L++ L H I QV CG+ H +AL+++ ++F+WG N+ GQLGLG +P +K+L+ +P+ ITAG F L+ SG ++ WG N GQLG + ++P +K LR Q +VY+S G H AAL+ GG+FT+G G GQLGH + N+ +P VM+L+G++VSQI CG +HTLA++ S+GTVYAFG GQLG ++ + P+ ++ L + Y V ++ GG+HS + SS C + P+D R++N P N +N + +Y + S + V SS AC ++SFLD + H IDL N + F++ S + + + S + Q R+ P +E +R +LI + L+ S+ + IP +++ + PS L+ W + F + ++ + + + G G+ L KLH++N + + YN FYIP I + D++ Y W + ++I+ +L +PF+ + KT +++ +A+ M + P + VRR++L+ DT+ +L + LKK +V F GE+A+D GG+ KE FLL++ ++++P YG+F Y ES L WF+ F + F+ IG + GLAIYN+ ++DLHFPL ++KKLL+ +L+D EL+PT +SLQ LLDY+ D E F+LNF ++ E YG EL+ G V +NR+ +VD Y+ Y+ + +V++ + F GF G+ L F+P EL ++VG+ +Y+W L K+ Y GEY H + FW F ++KK+FL +LTG DR+PI+GM ++I IQ +++YLPVAHTCYNLLD+P+Y ++ ILR +L A+E EGF+LV
Sbjct: 1 MLCWGNARDGQLGIGVEGNPVFE-PRICHVFSGRGLKEVACGGQHTLFLLQDGSVYTCGSNSCGQLGHDKAGNSPELVGALDTQKITMVSCGRAHSVAVNEQGQVFVWGAGDGGQLGLET-AETAVRIPRLLKKLCDHSISQVVCGNQHCIALSRDGQLFTWGQNSSGQLGLGKGETSKLSPSPVKSLAGIPLAQITAGGDHCFALSLSGAVFGWGKNKAGQLGLN-DKQDRAVPCHIKFLRSQRVVYISCGDEHTAALTKSGGLFTFGDGSRGQLGHGSSNNELLPRRVMELMGTEVSQITCGRHHTLAFVPSSGTVYAFGCNSHGQLGTGILADL-RSPHPVKNDFLTLNLRKDLR---------KYTVIKIICGGDHSFLMYSSEQCPE--VPEDFRVFNVSKSLSPINHDCLNSWRLKLLYNTDSNVTKDMIVHLSSTACWNASFLDQSDDA-HFKTNPKIPGIDL--NSVRMLFATLSKPAFAGIQEQATTSFESLLIPQLPRSPPDVEAMRIYLILSEYPALQDSKNYIRLTIPLAIAILQLDTNPSKVLDNWWCVVDVGVFTRMVDMYKSIVVYMLTG--GKTVPAPVLHENYFMATLRLLEKLHKVNLKAHHV-EYNHFYIPDITSLVDIQEDYLKWFLSKAQIKVGSSPSQSNLPPVNLCAFPFILNAQAKTTMLQTDAELQMQMAVSGANLHNVFMLLTLEPHLARNPYLVLHVRRNHLVSDTLRELTVYSDVDLKKPLKVIFDGEEAVDAGGLTKEFFLLLLKELMDPVYGMFTYYKESNLLWFSDKCFVEQ-NWFHLIGIICGLAIYNSTVVDLHFPLALYKKLLDVSPSLEDFKELSPTEARSLQQLLDYEGRDVEGTFLLNFAITRENYGMTEVKELVPGGESIAVDNTNRKEFVDSYLRYVFSDSVDEQYSAFSSGFLKVCGGEILSLFQPSELMAMVVGNNNYNWEELEKNAVYKGEYSATHPTVKLFWEVFHEFPLEKKKQFLLFLTGSDRIPIHGMESLRIIIQSTTAEEHYLPVAHTCYNLLDMPRYQTKEILRRRLTQAVEQYEGFSLV 1047
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Match: herc3 (HECT and RLD domain containing E3 ubiquitin protein ligase 3 [Source:NCBI gene;Acc:101155493]) HSP 1 Score: 471.855 bits (1213), Expect = 1.128e-148 Identity = 314/966 (32.51%), Postives = 505/966 (52.28%), Query Frame = 2
Query: 104 IFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIK---LVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKH---PTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQI--QYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEF------------SLFDYPFLFDGITKTNLMKIEAKCLMSEGL---------------PCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRK 2896
+ +G A GQLGI + P+ S +++++CG HTLFLL++G +Y+CG+N +LGHD P + LD K+ S G H+ V G VF WG G+LG + ++ +++ L++ L H I QV CG+ H +AL+++ ++F+WG N+ GQLGLG +P +K+L+ +P+ ITAG F L+ SG ++ WG N GQLG + ++P +K LR Q +VY+S G H AAL+ GG+FT+G G GQLGH + N+ +P VM+L+G++VSQI CG +HTLA++ S+GTVYAFG GQLG ++ + P+ ++ L + Y V ++ GG+HS + SS C + P+D R++N P N +N + +Y + S + V SS AC ++SFLD + H IDL N + F++ S + + + S + Q R+ P +E +R +LI + L+ S+ + IP +++ + PS L+ W + F + ++ + + + G G+ L KLH++N + + YN FYIP I + D++ Y W + ++I+ +L +PF+ + KT +++ +A+ M + P + VRR++L+ DT+ +L + LKK +V F GE+A+D GG+ KE FLL++ ++++P YG+F Y ES L WF+ F + F+ IG + GLAIYN+ ++DLHFPL ++KKLL+ +L+D EL+PT +SLQ LLDY+ D E F+LNF ++ E YG EL+ G V +NR+ +VD Y+ Y+ + +V++ + F GF G+ L F+P EL ++VG+ +Y+W L K
Sbjct: 1 MLCWGNARDGQLGIGVEGNPVFE-PRICHVFSGRGLKEVACGGQHTLFLLQDGSVYTCGSNSCGQLGHDKAGNSPELVGALDTQKITMVSCGRAHSVAVNEQGQVFVWGAGDGGQLGLET-AETAVRIPRLLKKLCDHSISQVVCGNQHCIALSRDGQLFTWGQNSSGQLGLGKGETSKLSPSPVKSLAGIPLAQITAGGDHCFALSLSGAVFGWGKNKAGQLGLN-DKQDRAVPCHIKFLRSQRVVYISCGDEHTAALTKSGGLFTFGDGSRGQLGHGSSNNELLPRRVMELMGTEVSQITCGRHHTLAFVPSSGTVYAFGCNSHGQLGTGILADL-RSPHPVKNDFLTLNLRKDLR---------KYTVIKIICGGDHSFLMYSSEQCPE--VPEDFRVFNVSKSLSPINHDCLNSWRLKLLYNTDSNVTKDMIVHLSSTACWNASFLDQSDDA-HFKTNPKIPGIDL--NSVRMLFATLSKPAFAGIQEQATTSFESLLIPQLPRSPPDVEAMRIYLILSEYPALQDSKNYIRLTIPLAIAILQLDTNPSKVLDNWWCVVDVGVFTRMVDMYKSIVVYMLTG--GKTVPAPVLHENYFMATLRLLEKLHKVNLKAHHV-EYNHFYIPDITSLVDIQEDYLKWFLSKAQIKVGSSPSQSNLPPVNLCAFPFILNAQAKTTMLQTDAELQMQMAVSGANLHNVFMLLTLEPHLARNPYLVLHVRRNHLVSDTLRELTVYSDVDLKKPLKVIFDGEEAVDAGGLTKEFFLLLLKELMDPVYGMFTYYKESNLLWFSDKCFVEQ-NWFHLIGIICGLAIYNSTVVDLHFPLALYKKLLDVSPSLEDFKELSPTEARSLQQLLDYEGRDVEGTFLLNFAITRENYGMTEVKELVPGGESIAVDNTNRKEFVDSYLRYVFSDSVDEQYSAFSSGFLKVCGGEILSLFQPSELMAMVVGNNNYNWEELEK 944
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Match: ENSORLT00000015298.2 (probable E3 ubiquitin-protein ligase HERC4 [Source:NCBI gene;Acc:101161054]) HSP 1 Score: 312.383 bits (799), Expect = 9.764e-90 Identity = 269/957 (28.11%), Postives = 452/957 (47.23%), Query Frame = 2
Query: 512 IVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXX------XDIKNSVFHISQTNHGLNI---DLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQ---------YNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNK-----------WIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKF-------------SDIIEMKPVFD---CLCKLHQ--INED--------HNRIIHYNTFYIPKIPKVFDLEYSYELWLR--NVSKIEFSLFD-----YPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVD--DNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNE 3190
+ QVACG HT+ALTK ++++WG N+ GQLGLG +S+PQ +++LS +P+ ++AG SF L+ SG ++ WG N+ GQLG + P V L + +S G++H A L+ G VFT+G G++GQLGHN+ N+ P +V +L G+KV++I CG HTL + +Y+FG QLG + H + VPL I G ++ ++ G N S + L +K + D N + N + ++ +TS S MK+ + SS+ L+ K+ F S HGL++ L+F + + K N++ +++ ++N +E LR +L+ ++E + + + E + A+ + W +L P + V+ A++ ++ +G +T S + E + + D CL KL + ++ED H I+ +P V DL+ ++ + + +K+E +L D + +++D T+ NL P M + +RR ++L DT QL Q KKS V F DE +K+ F V ++++P+YG+F L WF ++ +F G L GLA+YN V+I L FPLV+FKKLL +L+DL E +P+ K+L +L ++ D + N + + +K +L +KPVT N++ +VD YV+Y N +V++ F+ F GF + +K F+P EL +VG++ ++W L+++ Y G Y +H I FW F LS ++K F+ ++TG +R+PI+ + I I + ++ D D Y P + TC+ LL LP Y+S+ I+ +KL A+ +N
Sbjct: 119 VSQVACGSRHTVALTKEGQVYTWGQNSRGQLGLGKKGCSISSPQCVRSLSGMPLVQVSAGGDHSFALSVSGSVFGWGRNDCGQLGLG-DSQDRCTPTSVDSLNLKKTTSISCGKDHTAILTKHGTVFTFGSGQFGQLGHNSSRNELRPRLVAELWGAKVTRIACGRYHTLV-LQENNKIYSFGCNDQNQLGRSND---SHPS-------VPLPVLLPQDIGGTK-------IRTIFAGENCSF---APLTTDEKFHRDS----NCDNANNKAQLCLENMVKRWTSESDMKTWKKTKQEIQRTFSSASCLNRSFLEKKDKYFQTSPKYHGLDLKRAQLSFKKLVK-----------------KKNVWEEVEAALLRLLPLLDKNPVGVEGLRIYLL--------LNELLRATQTQNPQQSTKLAEVVAAAVTRLSGKNLQVIGDWWSSLMPSRRVRHVNVWKKAVSAMLSSEDGSHTPAVRNLLLVLQYMYNVNSRVAECQRLPDSVFCL-KLSEAFLDEDGIRWYLRSHGEIMMSEPRVLPNFSFVMDLKAKMLIFQKMCDATKVEKALGDLSSLLWSYMWD--TQNNL-------------PGLM------LDLRRASVLEDTFEQLAATQQEDYKKSLVVIFDDNPVFDEV-YIKDFFYEVFHEIVSPEYGMFMFNDSKTLAWFPSKAQQED-QRFFLFGVLCGLALYNQVIIFLPFPLVLFKKLLGVTPSLEDLREFSPSEYKTLHCIL----YEYTDDVLENLFIDFQITWDKTCIDLDPAFPEKPVTGQNKKEFVDAYVNYAFNTSVKRIFQEFARGFFKVCDRELVKLFRPEELHRALVGNDFHEWEKLKQNTLYDGGYNPSHTNILMFWEVFDELSENQKMAFVWFVTGYERLPISALEKITISTKKISEDFGDRYYPESETCFRLLVLPLYSSKEIMTEKLVEALSSNR 996 HSP 2 Score: 140.584 bits (353), Expect = 2.545e-33 Identity = 86/276 (31.16%), Postives = 142/276 (51.45%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDK--LSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVM---DLLGSKVSQIECGMNHTLAYMVS 1036
CG D + L + G +Y + L P ++ + + G H +T G V+TWG+NS G+LG K S S + V+SL+G +VQV+ G DH+ AL+ + +F WG N CGQLGLGD+ D TP + +L+ SI+ G + +LT G ++++GS +GQLG + ++ E P +V L + ++ G+ H L + ++++G QLG +N + S+P V+ D+ G+K+ I G N + A + +
Sbjct: 82 CGDDEVILLSEKGSVYRVDVTQALYL--------PRMLEAFCRIPVSQVACGSRHTVALTKEGQVYTWGQNSRGQLGLGKKGCSISSPQCVRSLSGMPLVQVSAGGDHSFALSVSGSVFGWGRNDCGQLGLGDSQDRC-TPTSVDSLNLKKTTSISCGKDHTAILTKHGTVFTFGSGQFGQLGHN-SSRNELRPRLVAELWGAKVTRIACGRYHTLVLQENNKIYSFGCNDQNQLGRSNDSHPSVPLPVLLPQDIGGTKIRTIFAGENCSFAPLTT 347 HSP 3 Score: 118.627 bits (296), Expect = 1.276e-26 Identity = 81/272 (29.78%), Postives = 119/272 (43.75%), Query Frame = 2
Query: 302 GHDCNK------VKPNQIQMLD-AHKLIDASAGYDHNAVVTNLGLVFTW----GKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLG 1084
G DC + + +Q+L + + D SAG A G F G+N G+ K K I V CG D + L++ ++ D + P+ ++ +P+ + G + + LT G +Y+WG N+ GQLG S P V+ L P+V VS G +H ALS+ G VF WG GQLG + +++ P V L K + I CG +HT A + GTV+ FG G+FGQLG
Sbjct: 5 GEDCRRGFRLMDSGDDGVQLLHVSFHIADLSAGRGALAFAKTNGNAFVMRTSDGENGARMRGKQKFVKCKEK---------IEAVGCGDDEVILLSEKGSVYR-----------VDVTQALYLPRMLEAFCRIPVSQVACGSRHTVALTKEGQVYTWGQNSRGQLGLGKKGCSISSPQCVRSLSGMPLVQVSAGGDHSFALSVSGSVFGWGRNDCGQLGLGDSQDRCTPTSVDSLNLKKTTSISCGKDHT-AILTKHGTVFTFGSGQFGQLG 255
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Match: SMESG000033113.1 (SMESG000033113.1) HSP 1 Score: 2094.32 bits (5425), Expect = 0.000e+0 Identity = 1050/1053 (99.72%), Postives = 1051/1053 (99.81%), Query Frame = 2
Query: 47 LTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
LTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNK KPNQIQMLDAHKLIDASAGYDHNAVVT+LGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIR GYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV
Sbjct: 401 LTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKFKPNQIQMLDAHKLIDASAGYDHNAVVTDLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRGGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 1453
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Match: SMESG000033113.1 (SMESG000033113.1) HSP 1 Score: 2084.69 bits (5400), Expect = 0.000e+0 Identity = 1050/1054 (99.62%), Postives = 1051/1054 (99.72%), Query Frame = 2
Query: 44 RLTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
LTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNK KPNQIQMLDAHKLIDASAGYDHNAVVT+LGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIR GYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV
Sbjct: 116 ELTLYFKLNFEFIIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKFKPNQIQMLDAHKLIDASAGYDHNAVVTDLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRGGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 1169
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Match: SMESG000033113.1 (SMESG000033113.1) HSP 1 Score: 2027.68 bits (5252), Expect = 0.000e+0 Identity = 1034/1037 (99.71%), Postives = 1035/1037 (99.81%), Query Frame = 2
Query: 95 MISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVXXXXXXXXXXXXXDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
MISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNK KPNQIQMLDAHKLIDASAGYDHNAVVT+LGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIR GYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV
Sbjct: 1 MISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKFKPNQIQMLDAHKLIDASAGYDHNAVVTDLGLVFTWGKNSFGELGRDKLSDFSIKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKSISEVLFSSLACLSSSFLDIKNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEGQNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSKIEFSLFDYPFLFDGITKTNLMKIEAKCLMSEGLPCFMAQSSFHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFDDNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLECYGNKIEYELIRGGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIINWFWNSFSSLSFDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 1037 HSP 2 Score: 92.8189 bits (229), Expect = 1.004e-18 Identity = 65/195 (33.33%), Postives = 105/195 (53.85%), Query Frame = 2
Query: 80 IIILKMISIFSFGAASYGQLGIDPRNKDYYSYPQXXXXXXXXXXXXXXCGFDHTLFLLKNGKLYSCGNNEFKKLGHDCN---KVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFSIK-LVQSLTGHFIVQVACGHDHTLA-LTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQI 649
+ + K IFS+GA + GQLG+ N D S PQ+I +S+ I+ I+ G + L +G LYS G+N + +LG D + P ++ L ++ S G +H A ++ G VFTWG +G+LG + + SI +V L G + Q+ CG +HTLA + ++++G GQLG+ D ++ T + I
Sbjct: 151 LALTKNSEIFSWGANTCGQLGLG-DNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPI 344
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Match: SMESG000057228.1 (SMESG000057228.1) HSP 1 Score: 684.1 bits (1764), Expect = 0.000e+0 Identity = 396/1028 (38.52%), Postives = 584/1028 (56.81%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS-IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESL---PLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKS--ISEVXXXXXXXXXXXXXDI----KNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEG-QNTKKKFSDIIEMKPVFDCLCKLHQINEDHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSK---IEFSLFDYPFLFDGITKTNLMKIEAKCLMSEG---------LPCFMAQSS---------FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLE-CYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
CG HT+ L NG + G+N + G K +I + K + G + ++ G+++TWG N+ +LGR +F I++VQ L+ + I+QVACG H LAL+ +S +FSWG N GQ+G G+ VS P+ + +L LPIK I GD S +LT SG +Y+WG N++GQLG S E+L P+ +K ++ I Y+ VG H A L+ DGGVFT+G GRYGQLGH NFE++ P VMDL+GS V+Q+ CG TL Y+ + Y FG GQLGI E + ++ + L SK ++ ++++ +R+Y GGNHS I + K DD+R WN++ I ++N + E +K IS V + + + + KNS F +++ HG++ID ++ + ++ L + M +N+ + +NYP +ECLR FLI +S ++ + I +P + + P L+ WI LKPRYFKK + N + ++ E QN K I++ + L ++ INE +I+ Y FYIP I + D+E ++ W S + FS YPF+FD K+ L+++EA M + AQ S F I V R+ +L DT+ L +A+Q KK +++F E+A+D+GGV+KE FLLV+ +++ P GLF+ Y +S++C+F + + + DN+ + +G+L GLAIYN V+IDLHFPLV+FKKLL E ++LDDL+EL+P LG SLQ +LDY NDF+D+F +S E G +EL+ +G PVT N++ +V Y+ +IL+KNV F F+ GF+ G LK F+PIELQE+MVG E +W L+ + Y G+YW HQ+I WFW F L+ ++KK+FLR+LTGCDR PI G++D KI IQP N D + PVAHTCYNLLDLPKY+S+ +L+ KL AIE+ EGF LV
Sbjct: 53 CGTSHTVMCLCNGTVLCAGDNSLLQFGKKAPKSHFEKISLFKEDKCCYVACGSHFSVALSTNGIIYTWGSNNANQLGRAMRDEFGKIEIVQCLSNYSIIQVACGLGHCLALSIDSILFSWGENKHGQIGNGNTSPFVSVPEIVLSLECLPIKQIACGDLHSSILTTSGSVYTWGDNSHGQLGTGKNGSEEALSSNPIFLKSIKGIQIAYICVGSFHNALLTHDGGVFTFGDGRYGQLGHGNFEDQFSPRKVMDLMGSTVTQLSCGNFFTLVYVTESKRFYGFGKSDEGQLGIGIHTEAVDSPTKLDDFLLDLSIKKNSKDKRKSISDKLNF-RRIYSGGNHSFILLTD---EKYKETDDMRNWNYETLKYIAMINESIIEEFQKPRDNIKDEIISTVYTAMGSLGCLAASFLDKSDKNSHFSMNKVKHGVDIDACHAFYNKMMKTFDESEMEMLRIHMYENLLQLRNFQQNYPGIECLRSFLILLISPMINNVNWYDTITVPTLTAINSFATVPRNYLDAWITKLKPRYFKKIVEMVTNLVKYHVSLLESKQNNKIPDKSQIQLTAALEFLKTIYMINE-KTKILSYTLFYIPTINEKIDIEKNFIDWATASSHYLPVSFSFCSYPFVFDAKAKSKLLQVEAGLFMRNAVLETHTNLIMSILNAQESGIINMETPFFEIGVNRETVLQDTLKALEKASQKEFKKPLKISFSNEEAVDDGGVVKEYFLLVIREIMLPKTGLFKCYNQSKMCYFQQNVKNKDNI--YRCVGQLCGLAIYNGVIIDLHFPLVLFKKLLGEISSLDDLVELDPVLGNSLQQVLDYTGNDFKDVFGFYHDISWEDIEGKSHIHELVTNGKSVPVTNDNKEQFVLDYIHFILDKNVASGFDAFKIGFYLVCVGSVLKLFRPIELQEMMVGCEINEWSELQNNAVYEGDYWKGHQVIIWFWEVFKDLNIEKKKQFLRFLTGCDRAPITGVTDFKIVIQPTNGGDQFYPVAHTCYNLLDLPKYSSKKVLKTKLLAAIEHTEGFTLV 1073 HSP 2 Score: 105.916 bits (263), Expect = 9.882e-23 Identity = 65/195 (33.33%), Postives = 102/195 (52.31%), Query Frame = 2
Query: 512 IVQVACGHDHTLALTKNSEIFSWGANTCGQLGL---GDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFEN-KSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLG 1084
+ VACG HT+ N + G N+ Q G +F+ +S ++ K + G FS L+ +G +Y+WGSNN QLGR + + + + V+ L I+ V+ G H ALSID +F+WG ++GQ+G+ N S+P IV+ L + QI CG H+ + + ++G+VY +G GQLG
Sbjct: 48 VKHVACGTSHTVMCLCNGTVLCAGDNSLLQFGKKAPKSHFEKISLFKEDKCC------YVACGSHFSVALSTNGIIYTWGSNNANQLGRAMRDEFGKIEI-VQCLSNYSIIQVACGLGHCLALSIDSILFSWGENKHGQIGNGNTSPFVSVPEIVLSLECLPIKQIACGDLHS-SILTTSGSVYTWGDNSHGQLG 234
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Match: SMESG000057228.1 (SMESG000057228.1) HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0 Identity = 395/1045 (37.80%), Postives = 584/1045 (55.89%), Query Frame = 2
Query: 224 CGFDHTLFLLKNGKLYSCGNNEFKKLGHDCNKVKPNQIQMLDAHKLIDASAGYDHNAVVTNLGLVFTWGKNSFGELGRDKLSDFS-IKLVQSLTGHFIVQVACGHDHTLALTKNSEIFSWGANTCGQLGLGDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESL---PLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFENKSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLGICDTVEICHTAKPINGHWVPLECNNQSKISGETTAEQMYIVKRLYGGGNHSIITASSLDCGKKVYPDDLRLWNWKHPTNITVMNVDVNEPIYTSISTMKS--ISEVXXXXXXXXXXXXXDI----KNSVFHISQTNHGLNIDLTFNIMSQFFSSQSSDYLQTLAVRMKDNIFSQIQYNRNYPSLECLRFFLIFAVSTILEISEFCKVILIPFSVSFSIIHETPSIALNKWIGNLKPRYFKKFCMVFINAINNEINGFEG-QNTKKKFSDIIEMKPVFDCLCKLHQINE-----------------DHNRIIHYNTFYIPKIPKVFDLEYSYELWLRNVSK---IEFSLFDYPFLFDGITKTNLMKIEAKCLMSEG---------LPCFMAQSS---------FHITVRRDNLLGDTISQLHRATQCHLKKSFRVNFMGEDALDEGGVMKELFLLVVPQVLNPDYGLFEMYPESRLCWFNKMIFD-DNLPQFNAIGRLFGLAIYNNVMIDLHFPLVMFKKLLNEKANLDDLIELNPTLGKSLQVLLDYDQNDFEDIFMLNFIVSLE-CYGNKIEYELIRDGYDKPVTLSNRQTYVDLYVDYILNKNVEQPFKYFQEGFHFTMQGKCLKFFKPIELQELMVGSESYDWLLLRKSCEYLGEYWNNHQIIXXXXXXXXXXXXDEKKKFLRYLTGCDRVPINGMSDIKIKIQPVNVDDNYLPVAHTCYNLLDLPKYTSESILRDKLKIAIENNEGFALV 3205
CG HT+ L NG + G+N + G K +I + K + G + ++ G+++TWG N+ +LGR +F I++VQ L+ + I+QVACG H LAL+ +S +FSWG N GQ+G G+ VS P+ + +L LPIK I GD S +LT SG +Y+WG N++GQLG S E+L P+ +K ++ I Y+ VG H A L+ DGGVFT+G GRYGQLGH NFE++ P VMDL+GS V+Q+ CG TL Y+ + Y FG GQLGI E + ++ + L SK ++ ++++ +R+Y GGNHS I + K DD+R WN++ I ++N + E +K IS V + + + + KNS F +++ HG++ID ++ + ++ L + M +N+ + +NYP +ECLR FLI +S ++ + I +P + + P L+ WI LKPRYFKK + N + ++ E QN K I++ + L ++ IN + +I+ Y FYIP I + D+E ++ W S + FS YPF+FD K+ L+++EA M + AQ S F I V R+ +L DT+ L +A+Q KK +++F E+A+D+GGV+KE FLLV+ +++ P GLF+ Y +S++C+F + + + DN+ + +G+L GLAIYN V+IDLHFPLV+FKKLL E ++LDDL+EL+P LG SLQ +LDY NDF+D+F +S E G +EL+ +G PVT N++ +V Y+ +IL+KNV F F+ GF+ G LK F+PIELQE+MVG E +W L+ + Y G+YW HQ+I WFW F L+ ++KK+FLR+LTGCDR PI G++D KI IQP N D + PVAHTCYNLLDLPKY+S+ +L+ KL AIE+ EGF LV
Sbjct: 53 CGTSHTVMCLCNGTVLCAGDNSLLQFGKKAPKSHFEKISLFKEDKCCYVACGSHFSVALSTNGIIYTWGSNNANQLGRAMRDEFGKIEIVQCLSNYSIIQVACGLGHCLALSIDSILFSWGENKHGQIGNGNTSPFVSVPEIVLSLECLPIKQIACGDLHSSILTTSGSVYTWGDNSHGQLGTGKNGSEEALSSNPIFLKSIKGIQIAYICVGSFHNALLTHDGGVFTFGDGRYGQLGHGNFEDQFSPRKVMDLMGSTVTQLSCGNFFTLVYVTESKRFYGFGKSDEGQLGIGIHTEAVDSPTKLDDFLLDLSIKKNSKDKRKSISDKLNF-RRIYSGGNHSFILLTD---EKYKETDDMRNWNYETLKYIAMINESIIEEFQKPRDNIKDEIISTVYTAMGSLGCLAASFLDKSDKNSHFSMNKVKHGVDIDACHAFYNKMMKTFDESEMEMLRIHMYENLLQLRNFQQNYPGIECLRSFLILLISPMINNVNWYDTITVPTLTAINSFATVPRNYLDAWITKLKPRYFKKIVEMVTNLVKYHVSLLESKQNNKIPDKSQIQLTAALEFLKTIYMINSTNRCLNSKRSNIPKNINEKTKILSYTLFYIPTINEKIDIEKNFIDWATASSHYLPVSFSFCSYPFVFDAKAKSKLLQVEAGLFMRNAVLETHTNLIMSILNAQESGIINMETPFFEIGVNRETVLQDTLKALEKASQKEFKKPLKISFSNEEAVDDGGVVKEYFLLVIREIMLPKTGLFKCYNQSKMCYFQQNVKNKDNI--YRCVGQLCGLAIYNGVIIDLHFPLVLFKKLLGEISSLDDLVELDPVLGNSLQQVLDYTGNDFKDVFGFYHDISWEDIEGKSHIHELVTNGKSVPVTNDNKEQFVLDYIHFILDKNVASGFDAFKIGFYLVCVGSVLKLFRPIELQEMMVGCEINEWSELQNNAVYEGDYWKGHQVIIWFWEVFKDLNIEKKKQFLRFLTGCDRAPITGVTDFKIVIQPTNGGDQFYPVAHTCYNLLDLPKYSSKKVLKTKLLAAIEHTEGFTLV 1091 HSP 2 Score: 105.916 bits (263), Expect = 9.586e-23 Identity = 65/195 (33.33%), Postives = 102/195 (52.31%), Query Frame = 2
Query: 512 IVQVACGHDHTLALTKNSEIFSWGANTCGQLGL---GDNFDMVSTPQQIKNLSNLPIKSITAGDKFSFVLTPSGFLYSWGSNNYGQLGRDLTTSYESLPLMVKHLRKQPIVYVSVGQNHVAALSIDGGVFTWGHGRYGQLGHNNFEN-KSIPCIVMDLLGSKVSQIECGMNHTLAYMVSTGTVYAFGLGKFGQLG 1084
+ VACG HT+ N + G N+ Q G +F+ +S ++ K + G FS L+ +G +Y+WGSNN QLGR + + + + V+ L I+ V+ G H ALSID +F+WG ++GQ+G+ N S+P IV+ L + QI CG H+ + + ++G+VY +G GQLG
Sbjct: 48 VKHVACGTSHTVMCLCNGTVLCAGDNSLLQFGKKAPKSHFEKISLFKEDKCC------YVACGSHFSVALSTNGIIYTWGSNNANQLGRAMRDEFGKIEI-VQCLSNYSIIQVACGLGHCLALSIDSILFSWGENKHGQIGNGNTSPFVSVPEIVLSLECLPIKQIACGDLHS-SILTTSGSVYTWGDNSHGQLG 234 The following BLAST results are available for this feature:
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of HECT and RLD domain containing E3 ubiquitin protein ligase 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005288 ID=SMED30005288|Name=HECT and RLD domain containing E3 ubiquitin protein ligase 4|organism=Schmidtea mediterranea sexual|type=transcript|length=3448bpback to top protein sequence of SMED30005288-orf-1 >SMED30005288-orf-1 ID=SMED30005288-orf-1|Name=SMED30005288-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1038bp MISIFSFGAASYGQLGIDPRNKDYYSYPQKIDKISSSDIEKISCGFDHTLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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