SMED30005212
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005212 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30005212 vs. TrEMBL
Match: K1P0G4 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10002070 PE=4 SV=1) HSP 1 Score: 71.2478 bits (173), Expect = 8.468e-10 Identity = 49/158 (31.01%), Postives = 76/158 (48.10%), Query Frame = 2 Query: 332 GLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRN----ISKSPWFVCPWDD---PRFRYCCGNEIEERCCRYFDRPGHLTGLLIG---GLVMVFIFVLVCQCFCKKHKHKEKADIALVNV-DPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQS 772 G + ++RYR Y +D +L E+ CYN+S Y R+ +S F CP D+ +RYCCG E ++ CC ++D+PGH+ G++ G G+V +F+ V + K K + N+ + Y A P + D N PYP S Sbjct: 165 GTVYGASRYRSRARYIADGTLPEV--CYNDS--YNRSTNGTVSYQGRFFCPLDENMSEDYRYCCGEEGQQYCCTFWDKPGHIVGVVFGIIAGVVALFVVVFCVIKYMKTTKSRNHNRPYTSNISNTSNGHASYPPAYAPPTYEMSCVDSNGKTPYPTS 318
BLAST of SMED30005212 vs. TrEMBL
Match: K1PDZ5 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10007565 PE=4 SV=1) HSP 1 Score: 60.4622 bits (145), Expect = 2.393e-7 Identity = 37/111 (33.33%), Postives = 55/111 (49.55%), Query Frame = 2 Query: 440 NISKSPWFVCPWDD---PRFRYCCGNEIEERCCRYFDRPGHLTGLLIG---GLVMVFIFVLVCQCFCKKHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNAC 754 +S F CP D+ +RYCCG E ++ CC ++D+PGH+ G++ G G+V +FI V C C + K D A + K + ++YPP PT AC Sbjct: 6 TVSYQGRFFCPIDEYMSEDYRYCCGEEGQQNCCTFWDKPGHIVGVVFGIIAGMVALFIAVF-CVVKCIRTKKSRNCDYASTVSNASKEQ------VIYPPVYAPPTYEIAC 109
BLAST of SMED30005212 vs. TrEMBL
Match: A0A1S3HM18 (uncharacterized protein LOC106155680 OS=Lingula unguis OX=7574 GN=LOC106155680 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 1.139e-6 Identity = 33/114 (28.95%), Postives = 56/114 (49.12%), Query Frame = 2 Query: 314 RNGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCP-WDDP-RFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVMVFIFVLVCQCFCKKHKHKEKA 649 + G + +RY+ Y D E + CYN + + N S F+CP WD P YCCG E+++ CC ++D G + G++IG L+ V + + C C + + + + Sbjct: 49 KTAAAVGTVYGLSRYQRRRRYKMDPDR-EPTRCYNSARRFQENGSLFNEFICPEWDQPDELEYCCGPELQQYCCGFWDDGGRVAGVVIGILIAVTLVIGCIACCCCQARKRRAS 161
BLAST of SMED30005212 vs. Planmine SMEST
Match: SMESG000042005.1 (SMESG000042005.1) HSP 1 Score: 507.294 bits (1305), Expect = 0.000e+0 Identity = 323/325 (99.38%), Postives = 325/325 (100.00%), Query Frame = 2 Query: 68 MLYXXXXXXXXXXXXXNCHTTWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKIGXXXXXXXXXXXHGXXXXXXXXXXXXXXKRNGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVMVFIFVLVCQCFCKKHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPYSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDIMK 1042 MLYLKIFWIFLFFFILNCHTTWSRGGRGGGSRGGSRSGSRSSGGFRSGTSGKIGSSSRYSMWSRSHGFSSSFRYRTSTYYYKRNGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVMVFIFVLVCQCFCKKHKHKEKADIALVNVDPDKI+IDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQP+SGQQQYNSLSYDESGPQYSLIPPENPPPSYSDIMK Sbjct: 1 MLYLKIFWIFLFFFILNCHTTWSRGGRGGGSRGGSRSGSRSSGGFRSGTSGKIGSSSRYSMWSRSHGFSSSFRYRTSTYYYKRNGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVMVFIFVLVCQCFCKKHKHKEKADIALVNVDPDKIDIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPHSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDIMK 325
BLAST of SMED30005212 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 155.221 bits (391), Expect = 1.074e-43 Identity = 101/242 (41.74%), Postives = 133/242 (54.96%), Query Frame = 2 Query: 317 NGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVM-VFIFVLVCQCFCK-KHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPYSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDI 1036 GI +GLI++S R T+ + S ++ISICYNE PLY RN+S SPWFVCPW DPR+RYCCG+EIEERCCRYFDRPGHL GL+IG +++ I++ + C KH + + + + P ++D N I+Y T+ + PYP DL + QP+F Q P PQN + D V QP Y S DES + L+ PENPPPSYS+I Sbjct: 98 TGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPKNAKLD-NNPIIY-------TENVSNLPYPTYNDLS---MEYQPEFPP----------QYPVDPQNPGS-----------DYVEQP---MPTYVS---DESNQHFPLLSPENPPPSYSEI 301
BLAST of SMED30005212 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 154.836 bits (390), Expect = 1.215e-43 Identity = 99/241 (41.08%), Postives = 132/241 (54.77%), Query Frame = 2 Query: 320 GINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVM-VFIFVLVCQCFCK-KHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPYSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDI 1036 GI +GLI++S R T+ + S ++ISICYNE PLY RN+S SPWFVCPW DPR+RYCCG+EIEERCCRYFDRPGHL GL+IG +++ I++ + C KH + + + + P ++D N I+Y T+ + PYP DL + QP+F Q P PQN + D V QP + DES + L+ PENPPPSYS+I Sbjct: 96 GIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPKNAKLD-NNPIIY-------TENVSNLPYPTYNDLS---MEYQPEFPP----------QYPVDPQNPGS-----------DYVEQPMP------TYVSDESNQHFPLLSPENPPPSYSEI 298
BLAST of SMED30005212 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 144.05 bits (362), Expect = 1.219e-39 Identity = 96/242 (39.67%), Postives = 125/242 (51.65%), Query Frame = 2 Query: 317 NGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVM-VFIFVLVCQCFCK-KHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPYSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDI 1036 GI +GLI++S R T+ + S ++ISICYNE PLY RN+S SPWFVCPW DPR+RYCCG+EIEERCCRYFDRPGHL GL+IG +++ I++ + C KH + + + + P + + PYP DL + QP+F Q P PQN + D V QP Y S DES + L+ PENPPPSYS+I Sbjct: 95 TGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPK-------------------NENVSNLPYPTYNDLS---MEYQPEFPP----------QYPVDPQNPGS-----------DYVEQP---MPTYVS---DESNQHFPLLSPENPPPSYSEI 287
BLAST of SMED30005212 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1) HSP 1 Score: 144.05 bits (362), Expect = 1.298e-39 Identity = 96/242 (39.67%), Postives = 125/242 (51.65%), Query Frame = 2 Query: 317 NGINSGLILVSARYRGTYYYSSDTSLDEISICYNESPLYGRNISKSPWFVCPWDDPRFRYCCGNEIEERCCRYFDRPGHLTGLLIGGLVM-VFIFVLVCQCFCK-KHKHKEKADIALVNVDPDKIEIDYTNAIVYPPEPVLPTDGNACPPYPQSFDLDKLPLCGQPDFSQTGITPNQTENQIPYVPQNTDNNGNPPYPLSPMDSVPQPYSGQQQYNSLSYDESGPQYSLIPPENPPPSYSDI 1036 GI +GLI++S R T+ + S ++ISICYNE PLY RN+S SPWFVCPW DPR+RYCCG+EIEERCCRYFDRPGHL GL+IG +++ I++ + C KH + + + + P + + PYP DL + QP+F Q P PQN + D V QP Y S DES + L+ PENPPPSYS+I Sbjct: 98 TGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPK-------------------NENVSNLPYPTYNDLS---MEYQPEFPP----------QYPVDPQNPGS-----------DYVEQP---MPTYVS---DESNQHFPLLSPENPPPSYSEI 290 The following BLAST results are available for this feature:
BLAST of SMED30005212 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005212 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005212 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of SMED30005212 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005212 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005212 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005212 ID=SMED30005212|Name=SMED30005212|organism=Schmidtea mediterranea sexual|type=transcript|length=1260bpback to top protein sequence of SMED30005212-orf-1 >SMED30005212-orf-1 ID=SMED30005212-orf-1|Name=SMED30005212-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=328bp MLYLKIFWIFLFFFILNCHTTWSRGGRGGGSRGGSRSGSRSSGGFRSGTSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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