Ankyrin repeat domain 50

Overview
NameAnkyrin repeat domain 50
Smed IDSMED30005209
Length (bp)3629
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ankyrin repeat domain 50 (SMED30005209) t-SNE clustered cells

Violin plots show distribution of expression levels for Ankyrin repeat domain 50 (SMED30005209) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ankyrin repeat domain 50 (SMED30005209) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ankyrin repeat domain 50 (SMED30005209) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005209

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30005209h1SMcG0021627 dd_Smed_v4_12936_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005209h1SMcG0021627 dd_Smed_v4_12936_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005209h1SMcG0021627 dd_Smed_v4_12936_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30005209h1SMcG0021627 dd_Smed_v6_12936_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 393/1038 (37.86%), Postives = 601/1038 (57.90%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDE--NLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE--VKGILPCEKFGSLNDWSIDDIVKILFESINLVN--------INKLNAKKSFTIFS----TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            LA HFC A D D+L +  FI  +   + +S    + Y   +    + +L         P  AFK  +LLPLL   PP +++Y+L+DS+DE  N+     GE+ S              S  ++ LL+ HH FFP WL L+C+ RK+++ + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+  E+Y A+   +++  L   +  L  +   LV    N     H S  EWLLDVK CT KY C+  +GH  LA+     +  L PL+ + FA HLI S    + +    A+W++++   +   +  ++P E+          +++++L ++   VN        I +   ++  +I +       +N   +   ++ A   YS SL         + ++ L+  G D+   + +G T L +AA+ G T + N L+  GAN    D+DGW+ALR A W G+ EVV  LL   +  D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NEIDN GRIP +L+ QEG+    + L++    +++ + YDG  +L++A L    + VE L S GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++ QLLI+ G  +D TC+QGATAL IA+QEGH+++  +L+  GA+  H D   RT  +VA K  H +I  LLEK+GA
Sbjct:  108 LAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLL-QGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITE---GEQTSTSL-----------SGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSN--LQLETAELALWMIWNGTPVRDSLSTLIPKEQ----------EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSL---------DVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN-KSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLE-----NGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGA 1102          

HSP 2 Score: 253.447 bits (646), Expect = 4.485e-68
Identity = 177/553 (32.01%), Postives = 288/553 (52.08%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            L+  G  +N  +E+  TS +V            LL +GA+    D +G + L  A ++G ++VV LL+S+  D +     G T L  AA  G T +V  L+  G+NIN  D +G T L +A +     +V  LL  G  +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A         E L+ +GA     DN+GR P  ++  +  YDC Q+LL+     N +NI+    DGR A+ +A+ +G  +I++ L   GA ++ KD +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  +LI + A+V   D+  R+  + A    H+K+  LL +HGA+VD
Sbjct:  500 LVKAGAHVN--SEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLE-----NKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVD 1039          

HSP 3 Score: 191.045 bits (484), Expect = 1.936e-48
Identity = 142/463 (30.67%), Postives = 229/463 (49.46%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC-----QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +++ LL+ +G+++N +D      +  A  + D +  + LL+NGA ++  D++GR+ +  AA      S  LD++ LL+    ++ + D  G   L +AA +G   +   L+   A+I+  D+   + L +AA+ G+ EVV  LL+ G  + C D    + L  +A  G+  I+  LL  G +    DN G T L  A+  G  E V  LL  GA+ N  D  GR  L ++       +G+  +   LID   E V+    DG T L +A      + V+ LL  GAD    DNNGRTPL  +           LL          ++ +D +GRT + IAS +G+ E++++L+D G   + +D+ G T LH+ A++G   IC+ LI+ G   +E  + G     +ASQEGH +   +L+   +N+       R   +VA    H  I  LL  HGA V+ K+ 
Sbjct:  495 EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSI--RTLLDNGASVNQCDSNGRTLLANAAY-----SGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE-VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAA-----VDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDA 944          

HSP 4 Score: 152.91 bits (385), Expect = 1.235e-36
Identity = 99/303 (32.67%), Postives = 164/303 (54.13%), Query Frame = 2
Query: 1670 QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            Q +   +  L+  GLD N  ++ G T L +AA  G   IC  L+  GA    ID DG     LA   G+ + V++LL  K + D  G DGR ALR AA  G  DIV LL S G+++N +D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      +++++++L+ Y+ ++N  DNE  + L  AA++G   + ++L+E+ A +D       + L  AA  G+ +VV++LL  GA  +  D+ G + + ++A  G+SQI+  L   G   ++    GC+P  + ++E
Sbjct:  824 QGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVS--CWQG---HMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLN----GCSPSPVHTME 1117          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 674.855 bits (1740), Expect = 0.000e+0
Identity = 370/963 (38.42%), Postives = 568/963 (58.98%), Query Frame = 2
Query:  491 PPNENMYILIDSIDE--NLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE--VKGILPCEKFGSLNDWSIDDIVKILFESINLVN--------INKLNAKKSFTIFS----TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            PP +++Y+L+DS+DE  N+     GE+ S              S  ++ LL+ HH FFP WL L+C+ RK+++ + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+  E+Y A+   +++  L   +  L  +   LV    N     H S  EWLLDVK CT KY C+  +GH  LA+     +  L PL+ + FA HLI S    + +    A+W++++   +   +  ++P E+          +++++L ++   VN        I +   ++  +I +       +N   +   ++ A   YS SL         + ++ L+  G D+   + +G T L +AA+ G T + N L+  GAN    D+DGW+ALR A W G+ EVV  LL   +  D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NEIDN GRIP +L+ QEG+    + L++    +++ + YDG  +L++A L    + VE L S GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++ QLLI+ G  +D TC+QGATAL IA+QEGH+++  +L+  GA+  H D   RT  +VA K  H +I  LLEK+GA
Sbjct:    3 PPQQSLYLLVDSVDEGCNITE---GEQTSTSL-----------SGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSN--LQLETAELALWMIWNGTPVRDSLSTLIPKEQ----------EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSL---------DVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN-KSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLE-----NGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGA 923          

HSP 2 Score: 253.832 bits (647), Expect = 1.892e-68
Identity = 177/553 (32.01%), Postives = 288/553 (52.08%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            L+  G  +N  +E+  TS +V            LL +GA+    D +G + L  A ++G ++VV LL+S+  D +     G T L  AA  G T +V  L+  G+NIN  D +G T L +A +     +V  LL  G  +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A         E L+ +GA     DN+GR P  ++  +  YDC Q+LL+     N +NI+    DGR A+ +A+ +G  +I++ L   GA ++ KD +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  +LI + A+V   D+  R+  + A    H+K+  LL +HGA+VD
Sbjct:  321 LVKAGAHVN--SEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLE-----NKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVD 860          

HSP 3 Score: 191.43 bits (485), Expect = 1.218e-48
Identity = 142/463 (30.67%), Postives = 229/463 (49.46%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC-----QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +++ LL+ +G+++N +D      +  A  + D +  + LL+NGA ++  D++GR+ +  AA      S  LD++ LL+    ++ + D  G   L +AA +G   +   L+   A+I+  D+   + L +AA+ G+ EVV  LL+ G  + C D    + L  +A  G+  I+  LL  G +    DN G T L  A+  G  E V  LL  GA+ N  D  GR  L ++       +G+  +   LID   E V+    DG T L +A      + V+ LL  GAD    DNNGRTPL  +           LL          ++ +D +GRT + IAS +G+ E++++L+D G   + +D+ G T LH+ A++G   IC+ LI+ G   +E  + G     +ASQEGH +   +L+   +N+       R   +VA    H  I  LL  HGA V+ K+ 
Sbjct:  316 EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSI--RTLLDNGASVNQCDSNGRTLLANAAY-----SGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE-VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAA-----VDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDA 765          

HSP 4 Score: 153.295 bits (386), Expect = 8.479e-37
Identity = 99/303 (32.67%), Postives = 164/303 (54.13%), Query Frame = 2
Query: 1670 QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            Q +   +  L+  GLD N  ++ G T L +AA  G   IC  L+  GA    ID DG     LA   G+ + V++LL  K + D  G DGR ALR AA  G  DIV LL S G+++N +D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      +++++++L+ Y+ ++N  DNE  + L  AA++G   + ++L+E+ A +D       + L  AA  G+ +VV++LL  GA  +  D+ G + + ++A  G+SQI+  L   G   ++    GC+P  + ++E
Sbjct:  645 QGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVS--CWQG---HMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLN----GCSPSPVHTME 938          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 229.95 bits (585), Expect = 2.162e-60
Identity = 166/579 (28.67%), Postives = 278/579 (48.01%), Query Frame = 2
Query: 1604 STYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S YS S   ++  + S   + R          I NG+DIN  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L ++++EGH ++   L++ GA++        TP  VA K   +++ NLL +  A  D
Sbjct:   24 SDYSRSSRKSDANA-SYLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPD 586          

HSP 2 Score: 210.69 bits (535), Expect = 2.228e-54
Identity = 169/638 (26.49%), Postives = 289/638 (45.30%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD----------------------------DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNH---IKICNLLEKHGALVDRK 3337
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L ++      +   FLL  GA  +I    G TPLH++    + +   +LLQ S + +    + L                              +G T +HIA+ K   +I  +L++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L+N GA   H+D+  +  Y   + G H   IKI N L +H A V+ K
Sbjct:  120 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGA---HVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAK 753          

HSP 3 Score: 204.142 bits (518), Expect = 2.107e-52
Identity = 152/551 (27.59%), Postives = 257/551 (46.64%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L +SA +G+  + + LL  G         G TPLH+A+  G+ E  NLLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  271 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTLK 810          

HSP 4 Score: 132.109 bits (331), Expect = 3.593e-30
Identity = 104/398 (26.13%), Postives = 186/398 (46.73%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVV+ LL  GA+     + GF+ L+++  QG+ Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  +       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   71 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDT----KGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRA-AAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 458          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 228.794 bits (582), Expect = 4.504e-60
Identity = 160/547 (29.25%), Postives = 268/547 (48.99%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+DIN  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L ++++EGH ++   L++ GA++        TP  VA K   +++ NLL +  A  D
Sbjct:   44 IKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPD 575          

HSP 2 Score: 217.624 bits (553), Expect = 1.479e-56
Identity = 163/618 (26.38%), Postives = 288/618 (46.60%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L ++      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL+   ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 720          

HSP 3 Score: 203.756 bits (517), Expect = 2.907e-52
Identity = 152/551 (27.59%), Postives = 257/551 (46.64%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L +SA +G+  + + LL  G         G TPLH+A+  G+ E  NLLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTLK 799          

HSP 4 Score: 162.54 bits (410), Expect = 1.606e-39
Identity = 130/465 (27.96%), Postives = 213/465 (45.81%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G +      L L++   + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG TPLH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-----PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALGIARRLGYISVVDTL 798          

HSP 5 Score: 132.109 bits (331), Expect = 3.787e-30
Identity = 104/398 (26.13%), Postives = 186/398 (46.73%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVV+ LL  GA+     + GF+ L+++  QG+ Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  +       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDT----KGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRA-AAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 447          

HSP 6 Score: 129.413 bits (324), Expect = 2.533e-29
Identity = 110/408 (26.96%), Postives = 198/408 (48.53%), Query Frame = 2
Query: 2120 HQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIP-LMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQIC-QLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            H+   G+ +   A+    + + +L+     ++   +IN+ +  G+N L +A+ EG   +   LL+  A++D   +   + L  A+ +G  EVV++L+  GA ++   ++GF+ L ++A + + +++  LL  G  +      G TPL +A  +G  + V+LLL+     N+     R+P L ++ ++   K A  L+     + + +S  G T L IA           LL+R A       N  TPLH++  +   +  ++LL          I+    DG T +H  +  G  ++++ L+D  A I  K   G + LH+ A QG +  C QLL+   + +D+  +   TAL +A+  GH ++  +L++  AN         TP  +A K N IK+  LL KHGA +
Sbjct:   14 HRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLE-----NDTKGKVRLPALHIAARKDDTKAAALLLQ-NDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGA-----KIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHM-ATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 409          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 226.868 bits (577), Expect = 2.987e-59
Identity = 160/547 (29.25%), Postives = 268/547 (48.99%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+DIN  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L ++++EGH ++   L++ GA++        TP  VA K   +++ NLL +  A  D
Sbjct:   61 IKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDK-KANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPD 592          

HSP 2 Score: 215.312 bits (547), Expect = 1.181e-55
Identity = 172/664 (25.90%), Postives = 300/664 (45.18%), Query Frame = 2
Query: 1526 INLVNINKLNAKK--SFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKI-----------------------------DPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            IN+ N N LNA    S       +  +    +N   AT   + +L  +        +  L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                                 + D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L ++      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL+   ANV   +    TP  +A + + + +  +L   GA VD
Sbjct:   67 INICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ-QGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASP-----DAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVD 724          

HSP 3 Score: 201.06 bits (510), Expect = 2.908e-51
Identity = 152/551 (27.59%), Postives = 257/551 (46.64%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L +SA +G+  + + LL  G         G TPLH+A+  G+ E  NLLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  277 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLD-QGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTLK 816          

HSP 4 Score: 162.54 bits (410), Expect = 1.868e-39
Identity = 130/465 (27.96%), Postives = 213/465 (45.81%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G +      L L++   + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG TPLH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  362 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-----PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAH-VDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALGIARRLGYISVVDTL 815          

HSP 5 Score: 134.035 bits (336), Expect = 1.013e-30
Identity = 108/419 (25.78%), Postives = 196/419 (46.78%), Query Frame = 2
Query: 2096 LNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            + NG  I+  + +G +A+ +A     S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVV+ LL  GA+     + GF+ L+++  QG+ Q++S LL        R       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  +       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   61 IKNGVDINICNQNGLNALHLA-----SKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR----LPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNR-AAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 464          

HSP 6 Score: 129.798 bits (325), Expect = 2.219e-29
Identity = 102/387 (26.36%), Postives = 175/387 (45.22%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLPEF-----------VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLID---------------------------------FGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            + AA +G+ E     +  G  I+  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++ + L+D    S +  + DG T L +A+                    RLP                LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D                                   + +D+  +   TAL +A+  GH ++  +L++  AN         TP  +A K N IK+  LL KHGA +
Sbjct:   46 LRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDN-GASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 426          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Match: T28D6.4 (pep chromosome:WBcel235:III:11360456:11366214:1 gene:WBGene00012124.1 transcript:T28D6.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T28D6.4)

HSP 1 Score: 228.409 bits (581), Expect = 3.305e-61
Identity = 169/528 (32.01%), Postives = 268/528 (50.76%), Query Frame = 2
Query: 1751 LMVAAQNGFTSICNLLLSSGANQLMIDKDG-WSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAA-IHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPL-HISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGA-RIDLKDNEGRTGLHLGAWQGCYQICQLLIDF-GINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            L+ AA  G   +C  L     + +     G W+ALR A   G +E++ LLL + +D D  G+  RTALR AAW G+   V LLL +G+ ++++DSE RT LMAA + C   +V +++  GA ++  D  G +A+ +     +   +  +  KLLL +N +  + D  G   L +AAY G   L +++LE +  +D++D   ++PLM AA  G  E V+ L     A +  ID +G + L  +A  G+ +++  L + G DE H+DN G   LH A      E V  L        + D +G+ P+M++   G +K+ E L+ +  + +N   ++G T+L +A +      ++ +  R  D + +D++G TPL H  +L +     +V L H       N +  D  GRT +H+ +   D    K L  +G    +  D  GRT L    W     I   L+D  G++ + T  QGATALSIA+Q G  ++ NLL+ FGA     DS  RT   VA    +  I +LL+
Sbjct:  428 LLTAAARGNIEMCRFLADHFPHLVASSCCGEWNALRSAACYGQLEILHLLLEKGVDVDECGHSDRTALRAAAWSGQLAAVQLLLQTGAEVDRRDSEQRTALMAAAFMCHKDVVSVIIQYGADVNAIDKSGATALHLNLSNGSKQEENSETTKLLLEHNADCKIEDANGRVALHLAAYHGDPCL-QLILEKHPIVDVMDNMGQTPLMLAASQGQLEAVQFLTKTAKADVDAIDNNGRTALQWAAINGHPEVVEFLCSVGSDEGHKDNDGAVALHYAVTHDHVELVKHLTNQHTVEAK-DRYGQHPMMIAAANGNLKVLEYLMGI-SDIINQPDHEGRTALSLASMAAHTSIIDLISPRITDWSQRDHDG-TPLAHTLILSNLIFVAEVYLGHGA-----NSSDSDAHGRTCVHVVASTNDVAAAKMLKRIGGVNFEAVDRGGRTALMTAVWAKHVAISVFLLDSCGVDPNRTDRQGATALSIAAQIGDRDLVNLLLKFGAEPEIKDSMGRTAMDVAMISGNEPIVSLLQ 946          

HSP 2 Score: 126.716 bits (317), Expect = 3.231e-29
Identity = 131/516 (25.39%), Postives = 229/516 (44.38%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLM-----AATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLEN-NADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNIN--CLDDDGRTAIHIASWKGDGEIIKSLID-VGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            L+  G+D++    +  T+L  AA +G  +   LLL +GA                      EV           D   ++ RTAL  AA++   D+V +++  G+++N  D  G T L       +  + +    KLLL + A    +D +GR A+ +AA   +        ++L+L  +  ++++DN G   L++AA +G     + L +   AD+D +D   R+ L  AA +G+ EVV  L   G+     D  G   L  + +  + +++  L  +   E  +D +G  P+ +A+  G  + +  L+      N+ D+ GR  L L+    +  I + LI       + + +DG+      +L+ L    E  L  GA+++  D +GRT +H+    +     ++L +      I  +N   +D  GRTA+  A W     I   L+D  G   +  D +G T L + A  G   +  LL+ FG   +   S G TA+ +A   G+  I +LL
Sbjct:  477 LLEKGVDVDECGHSDRTALRAAAWSGQLAAVQLLLQTGA----------------------EV-----------DRRDSEQRTALMAAAFMCHKDVVSVIIQYGADVNAIDKSGATALHLNLSNGSKQEENSETTKLLLEHNADCKIEDANGRVALHLAAYHGDP------CLQLILEKHPIVDVMDNMGQTPLMLAASQGQLEAVQFLTKTAKADVDAIDNNGRTALQWAAINGHPEVVEFLCSVGSDEGHKDNDGAVALHYAVTHDHVELVKHLTNQHTVEA-KDRYGQHPMMIAAANGNLKVLEYLMGISDIINQPDHEGRTALSLASMAAHTSIID-LISPRITDWSQRDHDGTPLAHTLILSNLIFVAEVYLGHGANSSDSDAHGRTCVHVVASTNDVAAAKMLKR------IGGVNFEAVDRGGRTALMTAVWAKHVAISVFLLDSCGVDPNRTDRQGATALSIAAQIGDRDLVNLLLKFGAEPEIKDSMGRTAMDVAMISGNEPIVSLL 945          

HSP 3 Score: 105.145 bits (261), Expect = 1.760e-22
Identity = 121/453 (26.71%), Postives = 193/453 (42.60%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNG-AYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYD--GSTSLKIAVLNRLPEFVEFLL-SRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDV 3019
            + +S +I  G D+N ++++G T+L            +L LS+G+ Q                    E  KLLL    D      +GR AL  AA+ G    + L+L     ++  D+ G+TPLM A  +     V+ L     A +D  DN+GR+A+  AA+     + + ++++ L     +    DN+G   L  A       L K  L N   ++  DR    P+M AA +GN +V+  L+     I+  D  G + LS+++   ++ I+  +  R  D   RD+ G    H   +        + L  GA  ++ D HGR  + +      +  A+ L  +    VNF++ D  G T+L  AV  +      FLL S G D    D  G T L I+      D   +LL+      I      D  GRTA+ +A   G+  I+  L  V
Sbjct:  538 DVVSVIIQYGADVNAIDKSGATAL------------HLNLSNGSKQ----------------EENSETTKLLLEHNADCKIEDANGRVALHLAAYHG-DPCLQLILEKHPIVDVMDNMGQTPLMLAASQGQLEAVQFLTKTAKADVDAIDNNGRTALQWAAI-----NGHPEVVEFLCSVGSDEGHKDNDGAVALHYAVTHDHVELVK-HLTNQHTVEAKDRYGQHPMMIAAANGNLKVLEYLMGISDIINQPDHEGRTALSLASMAAHTSIIDLISPRITDWSQRDHDGTPLAHTLILSNLIFVAEVYLGHGANSSDSDAHGRTCVHVVASTNDVAAAKMLKRIG--GVNFEAVDRGGRTALMTAVWAKHVAISVFLLDSCGVDPNRTDRQGATALSIAAQIGDRDLVNLLLKFGAEPEIK-----DSMGRTAMDVAMISGNEPIVSLLQSV 948          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 196.438 bits (498), Expect = 3.019e-50
Identity = 172/638 (26.96%), Postives = 274/638 (42.95%), Query Frame = 2
Query: 1688 ISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVV-----------------------------KLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLD----------------------------DDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            ++ L+ NG ++N  + NG T L +AAQ     +   LL  GANQ +  +DG++ L +A+  G+  VV                              LLL  + +PD T   G T L  AA  G  ++  LLL  G+N+N Q     +PL  AT      M  LLL+ GA ID +  D  + +  AA                            L + +   ++D  + LL +   ++ +  + +  L +AA+ G   + K+LL+ +AD +   +   +PL  A      +VV LLL + AAI    +SG + L ++A  G   I+  LL +G +       G TPLHLA+   Q + V +L+++GAK +      + PL ++ + G   I   L+     S N  + D  + L IA      E    LL   AD T+    G TPLH++      +  ++LL+     +I   N +                              +G T +HIA+ K   EI  +L+   A  + K   G T LHL A +G  +I  LLI+ G ++    + G TA+ + +QE H+ +  +L N GA +    +   TP  VA     + +   L ++GA V  K
Sbjct:  115 VTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEK 751          

HSP 2 Score: 192.2 bits (487), Expect = 6.945e-49
Identity = 148/491 (30.14%), Postives = 231/491 (47.05%), Query Frame = 2
Query: 1745 TSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            T L VAA  G   +  LLL   A+      +G++ L +A     I+VV+LLL  +   + T   G T L  AA++G  +IV+ LL  G+N + +   G TPL  A       +V++L+ NGA +D Q  + ++ + +A+   N+     DI+ LLL+   N N    +  + L IAA EG + +  ILL++NAD  ++ +   +PL  A+  GN EVVRLLL  G  +    K+  + L ++A   N ++   LL  G         G TPLH+A+ + Q E  + LL+  A PN     G  PL LS QEG+ +I+  LI+                                   G+D   + NNG T +H+   +      Q+L      +N   IN   + G T +H+A   G   ++K L++ GA +  K     T LH  A QG     + L++ G + +E  + G T LSIA + G++ +   L
Sbjct:  361 TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNT-----DIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE----------------------------------NGSDVGAKANNGLTAMHLCAQEDHVPVAQILY-----NNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 3 Score: 184.111 bits (466), Expect = 1.837e-46
Identity = 159/566 (28.09%), Postives = 267/566 (47.17%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMA---ATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLAR--GLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            SL  + ++     + +LI     ++     G T+L +A+  G + I  +L+ +GAN  +   +G++ L +A    + EVVK LL    +   +  DG T L  A   G   +V +LL       + DS+G+  L A   A  K D     LLL N    D     G + + +AA     +  + ++ +LLL    N+N      ++ L +A   G   +  +LL   A ID   +   +PL  AA SG+ +VV LL+  GA I    K+G + L ++A   +      LL     +D++  D    TPLH+A+  G      LLL   A PN    +G  PL ++C++  IK+ E L+  +  ++   +  G T L +A        V +LL +GA+  ++   G TPLH++   +Q D  +VL++     N   ++    + +T +HIAS  G+ +I+  L+  GA  +    +  + LH+ A +G  ++  +L+D   +      +G T L +AS+ G+LE+  LL+  G  V        TP  VA   N+ K+  LL ++GA
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL-------ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAA-----HYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL--TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLK-YRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIR-----NGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGA 614          

HSP 4 Score: 183.726 bits (465), Expect = 2.434e-46
Identity = 155/558 (27.78%), Postives = 261/558 (46.77%), Query Frame = 2
Query: 1739 GTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSK------YLDI---------IKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            G+ S + AA+ G       LL +G +    + +G ++L LA   G+ EVV+ L+ ++   D     G TAL  A+  G++ IV +L+ +G+N+N Q   G TPL  A  +    +VK LL +GA       DG + + VA         A+ L ++SK       L I           LLL+   N ++    G   L IAA+ G + + ++LLE  A+++   R   SPL  A   G   +  LLL  GA I    K   + L  +A  G+ Q++  L+ +G     +   G  PLH+A+     +    LL   A  +++      PL ++   G++++A+ L+D   +  N ++ +G T L IA      + VE LL   A       +G TPLH++      +    LLQ     ++  +      G T +H+A+     ++++ LI  GA++D +  E +T LH+ +  G   I  LL+  G N + T     + L IA++EG  E+  +L++  A+   +     TP  +A K  ++++  LL + G  VD
Sbjct:   33 GSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVR-----GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVD 584          

HSP 5 Score: 182.185 bits (461), Expect = 8.368e-46
Identity = 175/623 (28.09%), Postives = 285/623 (45.75%), Query Frame = 2
Query: 1496 DDIVKILFE--SINLVNINKLN--AKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLII-NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDE--MHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            D +V +L E  S   V +  L+  AKK  T  +T +L       ++ + T+   F+         +  + QL++  G ++N    +  + L VA + G T++ NLLLS GA   +ID   KD  + L  A  +G+ +VV LL+ Q         +G   L  AA     D    LL   + ++    +  TPL  A + C +V V KLLL+  A     D + R+      L +      + +++LLL+Y   I      G+  L +AA+ G+  +   LL+  A+ D+      +PL  AA +   +VVR+L+  GA +    +   + L I++  GN+ I+  LL  G +     RDN+  +PLH+A+ EGQ E   +LL   A    +   G  PL L+ + G +++   L++     V+ +  +  T L +A      +    LL  GA A     NG TPLHI+  ++Q +    LLQ     N  +       G T +H+++ +G  EI   LI+ G+ +  K N G T +HL A +    + Q+L + G  I+   + G T L +A   G L +   L+  GA+V        TP   A +  H      L ++GA
Sbjct:  178 DRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQ----NEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGA---IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAH-CGHVRVAKLLLDRSA-----DPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY--SPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKS-----RAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGA 779          

HSP 6 Score: 174.096 bits (440), Expect = 2.723e-43
Identity = 150/546 (27.47%), Postives = 251/546 (45.97%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRI-PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            N  + L+  G  I+   ++  T L  AA++G   + +LL+  GA      K+G + L +A    +++  + LL  +   D    D  T L  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL   A I+     G + + VAA         ++I+  LL+   N ++    G   L +AA      + ++L+ N A +D   R   +PL  A+  GN ++V LLL  GA  +   +  +S L I+A +G  ++   LL    D+      G TPLHLAS  G  E V LLL+ G  P +I+   ++ PL ++      K+A  L++    S    + +G T L IA      E    LL   AD   +   G TPLH+S  +   +   +L++     N +++    ++G TA+H+ + +    + + L + GA I+ K N G T LH+    G   + + L++ G ++ E      T L  A+Q+GH      L+  GA+     +  +TP  +A +  ++ +   L
Sbjct:  274 NMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFM-----GAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGT-PVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN-GASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE-----NGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 7 Score: 143.665 bits (361), Expect = 4.791e-34
Identity = 105/347 (30.26%), Postives = 166/347 (47.84%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            R N  + +  LI NG  ++       T L +A++ G T I  LLL +GAN     +D +S L +A   G  EV  +LL    D       G T L  A+  G  ++V LLL  G+ ++ +     TPL +AA Y  D V + LLL NGA       +G + + +AA         ++I   LL++  + N     G   L ++A EG K +  +L+EN +D+     + L A    A   +  V ++L   GA I+    +G++ L ++   G   ++  L+  G D   +     TPLH A+ +G   CV  LL++GA PNE    G+ PL ++ + GY+ + E L
Sbjct:  467 RANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAM-LLLENGASAKAAAKNGYTPLHIAA-----KKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 8 Score: 107.842 bits (268), Expect = 4.155e-23
Identity = 103/441 (23.36%), Postives = 196/441 (44.44%), Query Frame = 2
Query: 2030 SEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +EG    + A    D   V  LL  G  I+  + +G +++ +A     S   + ++++ L++    ++    +G   L IA+  G  L+  IL+EN A++++      +PL  AA   + EVV+ LL  GA      + GF+ L+++  QG+ ++++ LL    +   +       LH+A+ +       LLL++   P+     G  PL ++   G+  + + L++    +VN+++    + L +A           LLSRGA    +  +  TPLH +          +L+          I+    +G   +H+A+     +  ++L+   A +D    +  T LH+ A  G  ++ +LL+D   + +     G T L IA ++  +++  LL+ + A +        TP  VA     I I   L + GA  D + V
Sbjct:   31 AEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLA-----SKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL----ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK-GANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAP-----ISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV 456          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 196.438 bits (498), Expect = 3.019e-50
Identity = 172/638 (26.96%), Postives = 274/638 (42.95%), Query Frame = 2
Query: 1688 ISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVV-----------------------------KLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLD----------------------------DDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            ++ L+ NG ++N  + NG T L +AAQ     +   LL  GANQ +  +DG++ L +A+  G+  VV                              LLL  + +PD T   G T L  AA  G  ++  LLL  G+N+N Q     +PL  AT      M  LLL+ GA ID +  D  + +  AA                            L + +   ++D  + LL +   ++ +  + +  L +AA+ G   + K+LL+ +AD +   +   +PL  A      +VV LLL + AAI    +SG + L ++A  G   I+  LL +G +       G TPLHLA+   Q + V +L+++GAK +      + PL ++ + G   I   L+     S N  + D  + L IA      E    LL   AD T+    G TPLH++      +  ++LL+     +I   N +                              +G T +HIA+ K   EI  +L+   A  + K   G T LHL A +G  +I  LLI+ G ++    + G TA+ + +QE H+ +  +L N GA +    +   TP  VA     + +   L ++GA V  K
Sbjct:  115 VTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEK 751          

HSP 2 Score: 192.2 bits (487), Expect = 6.945e-49
Identity = 148/491 (30.14%), Postives = 231/491 (47.05%), Query Frame = 2
Query: 1745 TSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            T L VAA  G   +  LLL   A+      +G++ L +A     I+VV+LLL  +   + T   G T L  AA++G  +IV+ LL  G+N + +   G TPL  A       +V++L+ NGA +D Q  + ++ + +A+   N+     DI+ LLL+   N N    +  + L IAA EG + +  ILL++NAD  ++ +   +PL  A+  GN EVVRLLL  G  +    K+  + L ++A   N ++   LL  G         G TPLH+A+ + Q E  + LL+  A PN     G  PL LS QEG+ +I+  LI+                                   G+D   + NNG T +H+   +      Q+L      +N   IN   + G T +H+A   G   ++K L++ GA +  K     T LH  A QG     + L++ G + +E  + G T LSIA + G++ +   L
Sbjct:  361 TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNT-----DIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE----------------------------------NGSDVGAKANNGLTAMHLCAQEDHVPVAQILY-----NNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 3 Score: 184.111 bits (466), Expect = 1.837e-46
Identity = 159/566 (28.09%), Postives = 267/566 (47.17%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMA---ATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLAR--GLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            SL  + ++     + +LI     ++     G T+L +A+  G + I  +L+ +GAN  +   +G++ L +A    + EVVK LL    +   +  DG T L  A   G   +V +LL       + DS+G+  L A   A  K D     LLL N    D     G + + +AA     +  + ++ +LLL    N+N      ++ L +A   G   +  +LL   A ID   +   +PL  AA SG+ +VV LL+  GA I    K+G + L ++A   +      LL     +D++  D    TPLH+A+  G      LLL   A PN    +G  PL ++C++  IK+ E L+  +  ++   +  G T L +A        V +LL +GA+  ++   G TPLH++   +Q D  +VL++     N   ++    + +T +HIAS  G+ +I+  L+  GA  +    +  + LH+ A +G  ++  +L+D   +      +G T L +AS+ G+LE+  LL+  G  V        TP  VA   N+ K+  LL ++GA
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL-------ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAA-----HYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL--TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLK-YRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIR-----NGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGA 614          

HSP 4 Score: 183.726 bits (465), Expect = 2.434e-46
Identity = 155/558 (27.78%), Postives = 261/558 (46.77%), Query Frame = 2
Query: 1739 GTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSK------YLDI---------IKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            G+ S + AA+ G       LL +G +    + +G ++L LA   G+ EVV+ L+ ++   D     G TAL  A+  G++ IV +L+ +G+N+N Q   G TPL  A  +    +VK LL +GA       DG + + VA         A+ L ++SK       L I           LLL+   N ++    G   L IAA+ G + + ++LLE  A+++   R   SPL  A   G   +  LLL  GA I    K   + L  +A  G+ Q++  L+ +G     +   G  PLH+A+     +    LL   A  +++      PL ++   G++++A+ L+D   +  N ++ +G T L IA      + VE LL   A       +G TPLH++      +    LLQ     ++  +      G T +H+A+     ++++ LI  GA++D +  E +T LH+ +  G   I  LL+  G N + T     + L IA++EG  E+  +L++  A+   +     TP  +A K  ++++  LL + G  VD
Sbjct:   33 GSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP-NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVR-----GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVD 584          

HSP 5 Score: 182.185 bits (461), Expect = 8.368e-46
Identity = 175/623 (28.09%), Postives = 285/623 (45.75%), Query Frame = 2
Query: 1496 DDIVKILFE--SINLVNINKLN--AKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLII-NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDE--MHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            D +V +L E  S   V +  L+  AKK  T  +T +L       ++ + T+   F+         +  + QL++  G ++N    +  + L VA + G T++ NLLLS GA   +ID   KD  + L  A  +G+ +VV LL+ Q         +G   L  AA     D    LL   + ++    +  TPL  A + C +V V KLLL+  A     D + R+      L +      + +++LLL+Y   I      G+  L +AA+ G+  +   LL+  A+ D+      +PL  AA +   +VVR+L+  GA +    +   + L I++  GN+ I+  LL  G +     RDN+  +PLH+A+ EGQ E   +LL   A    +   G  PL L+ + G +++   L++     V+ +  +  T L +A      +    LL  GA A     NG TPLHI+  ++Q +    LLQ     N  +       G T +H+++ +G  EI   LI+ G+ +  K N G T +HL A +    + Q+L + G  I+   + G T L +A   G L +   L+  GA+V        TP   A +  H      L ++GA
Sbjct:  178 DRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQ----NEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGA---IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAH-CGHVRVAKLLLDRSA-----DPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY--SPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER-GTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKS-----RAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGA 779          

HSP 6 Score: 174.096 bits (440), Expect = 2.723e-43
Identity = 150/546 (27.47%), Postives = 251/546 (45.97%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRI-PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            N  + L+  G  I+   ++  T L  AA++G   + +LL+  GA      K+G + L +A    +++  + LL  +   D    D  T L  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL   A I+     G + + VAA         ++I+  LL+   N ++    G   L +AA      + ++L+ N A +D   R   +PL  A+  GN ++V LLL  GA  +   +  +S L I+A +G  ++   LL    D+      G TPLHLAS  G  E V LLL+ G  P +I+   ++ PL ++      K+A  L++    S    + +G T L IA      E    LL   AD   +   G TPLH+S  +   +   +L++     N +++    ++G TA+H+ + +    + + L + GA I+ K N G T LH+    G   + + L++ G ++ E      T L  A+Q+GH      L+  GA+     +  +TP  +A +  ++ +   L
Sbjct:  274 NMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFM-----GAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGT-PVDIEGKNQVTPLHVAAHYNNDKVAMLLLEN-GASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE-----NGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 7 Score: 143.665 bits (361), Expect = 4.791e-34
Identity = 105/347 (30.26%), Postives = 166/347 (47.84%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            R N  + +  LI NG  ++       T L +A++ G T I  LLL +GAN     +D +S L +A   G  EV  +LL    D       G T L  A+  G  ++V LLL  G+ ++ +     TPL +AA Y  D V + LLL NGA       +G + + +AA         ++I   LL++  + N     G   L ++A EG K +  +L+EN +D+     + L A    A   +  V ++L   GA I+    +G++ L ++   G   ++  L+  G D   +     TPLH A+ +G   CV  LL++GA PNE    G+ PL ++ + GY+ + E L
Sbjct:  467 RANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAM-LLLENGASAKAAAKNGYTPLHIAA-----KKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 8 Score: 107.842 bits (268), Expect = 4.155e-23
Identity = 103/441 (23.36%), Postives = 196/441 (44.44%), Query Frame = 2
Query: 2030 SEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +EG    + A    D   V  LL  G  I+  + +G +++ +A     S   + ++++ L++    ++    +G   L IA+  G  L+  IL+EN A++++      +PL  AA   + EVV+ LL  GA      + GF+ L+++  QG+ ++++ LL    +   +       LH+A+ +       LLL++   P+     G  PL ++   G+  + + L++    +VN+++    + L +A           LLSRGA    +  +  TPLH +          +L+          I+    +G   +H+A+     +  ++L+   A +D    +  T LH+ A  G  ++ +LL+D   + +     G T L IA ++  +++  LL+ + A +        TP  VA     I I   L + GA  D + V
Sbjct:   31 AEGSASFLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLA-----SKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL----ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK-GANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAP-----ISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV 456          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 194.897 bits (494), Expect = 6.795e-50
Identity = 173/638 (27.12%), Postives = 273/638 (42.79%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVV-----------------------------KLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDND----------------------------GRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGR---IPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD----------------------------DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ NG ++N  + NG T L +AAQ     +   LL  GANQ +  +DG++ L +A+  G+  VV                              LLL  + +PD T   G T L  AA  G  ++  LLL  G+N+N Q     +PL  AT      M  LLL+ GA ID +  D                             ++   +A L + +   ++D  + LL +   ++ +  + +  L +AA+ G   + K+LL+ +AD +   +   +PL  A      +VV LLL + AAI    +SG + L ++A  G   I+  LL +G +       G TPLHLA+   Q + V +L+++GAK   +D   R    PL ++ + G   I   L+     S N  + D  + L IA      E    LL   AD T+    G TPLH++      +  ++LL+     +I   N +                              +G T +HIA+ K   EI  +L+   A  + K   G T LHL A +G  +I  LLI+ G ++    + G TA+ + +QE H+ +  +L N GA +    +   TP  VA     + +   L ++GA V  K
Sbjct:  118 LVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAK---VDAQARELQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEK 751          

HSP 2 Score: 193.741 bits (491), Expect = 1.484e-49
Identity = 148/491 (30.14%), Postives = 231/491 (47.05%), Query Frame = 2
Query: 1745 TSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            T L VAA  G   +  LLL   A+      +G++ L +A     I+VV+LLL  +   + T   G T L  AA++G  +IV+ LL  G+N + +   G TPL  A       +V++L+ NGA +D Q  + ++ + +A+   N+     DI+ LLL+   N N    +  + L IAA EG + +  ILL++NAD  ++ +   +PL  A+  GN EVVRLLL  G  +    K+  + L ++A   N ++   LL  G         G TPLH+A+ + Q E  + LL+  A PN     G  PL LS QEG+ +I+  LI+                                   G+D   + NNG T +H+   +      Q+L      +N   IN   + G T +H+A   G   ++K L++ GA +  K     T LH  A QG     + L++ G + +E  + G T LSIA + G++ +   L
Sbjct:  361 TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNT-----DIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE----------------------------------NGSDVGAKANNGLTAMHLCAQEDHVPVAQILY-----NNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 3 Score: 185.652 bits (470), Expect = 4.787e-47
Identity = 163/568 (28.70%), Postives = 269/568 (47.36%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMA---ATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL--ARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDET--CSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            SL  + ++     + +LI     ++     G T+L +A+  G + I  +L+ +GAN  +   +G++ L +A    + EVVK LL    +   +  DG T L  A   G   +V +LL +       DS+G+  L A   A  K D     LLL N    D     G + + +AA   + N     + +LLL    N+N      ++ L +A   G   +  +LL   A ID   +   +PL  AA SG+ +VV LL+  GA I    K+G + L ++A   +      LL     +D++  D    TPLH+A+  G      LLL   A PN    +G  PL ++C++  IK+ E L+  +  ++   +  G T L +A        V +LL +GA+  ++   G TPLH++   +Q D  +VL++     N   ++    + +T +HIAS  G+ +I+  L+  GA  +    +  + LH+ A +G  ++  +L+D   N D+T    +G T L +AS+ G+LE+  LL+  G  V        TP  VA   N+ K+  LL ++GA
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN-------DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHEN-----VGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL--TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLK-YRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIR-----NGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH--NADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGA 614          

HSP 4 Score: 182.956 bits (463), Expect = 2.815e-46
Identity = 175/623 (28.09%), Postives = 285/623 (45.75%), Query Frame = 2
Query: 1496 DDIVKILFE--SINLVNINKLN--AKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIIN-GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDE--MHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            D +V +L E  S   V +  L+  AKK  T  +T +L       ++ + T+   F+         +  + QL++  G ++N    +  + L VA + G T++ NLLLS GA   +ID   KD  + L  A  +G+ +VV LL+ Q         +G   L  AA     D    LL   + ++    +  TPL  A + C +V V KLLL+  A     D + R+      L +      + +++LLL+Y   I      G+  L +AA+ G+  +   LL+  A+ D+      +PL  AA +   +VVR+L+  GA +    +   + L I++  GN+ I+  LL  G +     RDN+  +PLH+A+ EGQ E   +LL   A    +   G  PL L+ + G +++   L++     V+ +  +  T L +A      +    LL  GA A     NG TPLHI+  ++Q +    LLQ        + N     G T +H+++ +G  EI   LI+ G+ +  K N G T +HL A +    + Q+L + G  I+   + G T L +A   G L +   L+  GA+V        TP   A +  H      L ++GA
Sbjct:  178 DRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQ----NEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGA---IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAH-CGHVRVAKLLLDRSA-----DPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY--SPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERG-TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKA-----DPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGA 779          

HSP 5 Score: 175.252 bits (443), Expect = 6.465e-44
Identity = 149/541 (27.54%), Postives = 249/541 (46.03%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRI-PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            L+  G  I+   ++  T L  AA++G   + +LL+  GA      K+G + L +A    +++  + LL  +   D    D  T L  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL   A I+     G + + VAA         ++I+  LL+   N ++    G   L +AA      + ++L+ N A +D   R   +PL  A+  GN ++V LLL  GA  +   +  +S L I+A +G  ++   LL    D+      G TPLHLAS  G  E V LLL+ G  P +I+   ++ PL ++      K+A  L++    S    + +G T L IA      E    LL   AD   +   G TPLH+S  +   +   +L++     N +++    ++G TA+H+ + +    + + L + GA I+ K N G T LH+    G   + + L++ G ++ E      T L  A+Q+GH      L+  GA+     +  +TP  +A +  ++ +   L
Sbjct:  279 LLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFM-----GAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGT-PVDIEGKNQVTPLHVAAHYNNDKVAMLLLENG-ASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE-----NGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 6 Score: 145.591 bits (366), Expect = 9.114e-35
Identity = 105/349 (30.09%), Postives = 167/349 (47.85%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAA---AFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            + R N  + +  LI NG  ++       T L +A++ G T I  LLL +GAN     +D +S L +A   G  EV  +LL    D       G T L  A+  G  ++V LLL  G+ ++ +     TPL +AA Y  D V + LLL NGA       +G + + +AA         ++I   LL++  + N     G   L ++A EG K +  +L+EN +D+     + L A    A   +  V ++L   GA I+    +G++ L ++   G   ++  L+  G D   +     TPLH A+ +G   CV  LL++GA PNE    G+ PL ++ + GY+ + E L
Sbjct:  465 AARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAM-LLLENGASAKAAAKNGYTPLHIAA-----KKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 7 Score: 104.375 bits (259), Expect = 3.753e-22
Identity = 104/441 (23.58%), Postives = 195/441 (44.22%), Query Frame = 2
Query: 2030 SEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +EG    + A    D   V  LL  G  I+  + +G     + +L L S   + ++++ L++    ++    +G   L IA+  G  L+  IL+EN A++++      +PL  AA   + EVV+ LL  GA      + GF+ L+++  QG+ ++++ LL    +   +       LH+A+ +       LLL++   P+     G  PL ++   G+  + + L++    +VN+++    + L +A           LLSRGA    +  +  TPLH +          +L+          I+    +G   +H+A+     +  ++L+   A +D    +  T LH+ A  G  ++ +LL+D   + +     G T L IA ++  +++  LL+ + A +        TP  VA     I I   L + GA  D + V
Sbjct:   31 AEGSASFLRAARAGDLEKVLELLRAGTDINTSNANG-----LNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL----ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKG-ANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAP-----ISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV 456          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 194.897 bits (494), Expect = 6.795e-50
Identity = 173/638 (27.12%), Postives = 273/638 (42.79%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVV-----------------------------KLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDND----------------------------GRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGR---IPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD----------------------------DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ NG ++N  + NG T L +AAQ     +   LL  GANQ +  +DG++ L +A+  G+  VV                              LLL  + +PD T   G T L  AA  G  ++  LLL  G+N+N Q     +PL  AT      M  LLL+ GA ID +  D                             ++   +A L + +   ++D  + LL +   ++ +  + +  L +AA+ G   + K+LL+ +AD +   +   +PL  A      +VV LLL + AAI    +SG + L ++A  G   I+  LL +G +       G TPLHLA+   Q + V +L+++GAK   +D   R    PL ++ + G   I   L+     S N  + D  + L IA      E    LL   AD T+    G TPLH++      +  ++LL+     +I   N +                              +G T +HIA+ K   EI  +L+   A  + K   G T LHL A +G  +I  LLI+ G ++    + G TA+ + +QE H+ +  +L N GA +    +   TP  VA     + +   L ++GA V  K
Sbjct:  118 LVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAK---VDAQARELQTPLHIASRLGNTDIVILLLQAGANS-NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEK 751          

HSP 2 Score: 193.741 bits (491), Expect = 1.484e-49
Identity = 148/491 (30.14%), Postives = 231/491 (47.05%), Query Frame = 2
Query: 1745 TSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            T L VAA  G   +  LLL   A+      +G++ L +A     I+VV+LLL  +   + T   G T L  AA++G  +IV+ LL  G+N + +   G TPL  A       +V++L+ NGA +D Q  + ++ + +A+   N+     DI+ LLL+   N N    +  + L IAA EG + +  ILL++NAD  ++ +   +PL  A+  GN EVVRLLL  G  +    K+  + L ++A   N ++   LL  G         G TPLH+A+ + Q E  + LL+  A PN     G  PL LS QEG+ +I+  LI+                                   G+D   + NNG T +H+   +      Q+L      +N   IN   + G T +H+A   G   ++K L++ GA +  K     T LH  A QG     + L++ G + +E  + G T LSIA + G++ +   L
Sbjct:  361 TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNT-----DIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE----------------------------------NGSDVGAKANNGLTAMHLCAQEDHVPVAQILY-----NNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 3 Score: 185.652 bits (470), Expect = 4.787e-47
Identity = 163/568 (28.70%), Postives = 269/568 (47.36%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMA---ATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL--ARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDET--CSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            SL  + ++     + +LI     ++     G T+L +A+  G + I  +L+ +GAN  +   +G++ L +A    + EVVK LL    +   +  DG T L  A   G   +V +LL +       DS+G+  L A   A  K D     LLL N    D     G + + +AA   + N     + +LLL    N+N      ++ L +A   G   +  +LL   A ID   +   +PL  AA SG+ +VV LL+  GA I    K+G + L ++A   +      LL     +D++  D    TPLH+A+  G      LLL   A PN    +G  PL ++C++  IK+ E L+  +  ++   +  G T L +A        V +LL +GA+  ++   G TPLH++   +Q D  +VL++     N   ++    + +T +HIAS  G+ +I+  L+  GA  +    +  + LH+ A +G  ++  +L+D   N D+T    +G T L +AS+ G+LE+  LL+  G  V        TP  VA   N+ K+  LL ++GA
Sbjct:   69 SLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN-------DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHEN-----VGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYL--TPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLK-YRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIR-----NGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDH--NADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGA 614          

HSP 4 Score: 182.956 bits (463), Expect = 2.815e-46
Identity = 175/623 (28.09%), Postives = 285/623 (45.75%), Query Frame = 2
Query: 1496 DDIVKILFE--SINLVNINKLN--AKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIIN-GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDE--MHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            D +V +L E  S   V +  L+  AKK  T  +T +L       ++ + T+   F+         +  + QL++  G ++N    +  + L VA + G T++ NLLLS GA   +ID   KD  + L  A  +G+ +VV LL+ Q         +G   L  AA     D    LL   + ++    +  TPL  A + C +V V KLLL+  A     D + R+      L +      + +++LLL+Y   I      G+  L +AA+ G+  +   LL+  A+ D+      +PL  AA +   +VVR+L+  GA +    +   + L I++  GN+ I+  LL  G +     RDN+  +PLH+A+ EGQ E   +LL   A    +   G  PL L+ + G +++   L++     V+ +  +  T L +A      +    LL  GA A     NG TPLHI+  ++Q +    LLQ        + N     G T +H+++ +G  EI   LI+ G+ +  K N G T +HL A +    + Q+L + G  I+   + G T L +A   G L +   L+  GA+V        TP   A +  H      L ++GA
Sbjct:  178 DRVVAVLLENDSKGKVRLPALHIAAKKDDTTAATLLLQ----NEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGA---IIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAH-CGHVRVAKLLLDRSA-----DPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNY--SPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERG-TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKA-----DPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGA 779          

HSP 5 Score: 175.252 bits (443), Expect = 6.465e-44
Identity = 149/541 (27.54%), Postives = 249/541 (46.03%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRI-PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            L+  G  I+   ++  T L  AA++G   + +LL+  GA      K+G + L +A    +++  + LL  +   D    D  T L  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL   A I+     G + + VAA         ++I+  LL+   N ++    G   L +AA      + ++L+ N A +D   R   +PL  A+  GN ++V LLL  GA  +   +  +S L I+A +G  ++   LL    D+      G TPLHLAS  G  E V LLL+ G  P +I+   ++ PL ++      K+A  L++    S    + +G T L IA      E    LL   AD   +   G TPLH+S  +   +   +L++     N +++    ++G TA+H+ + +    + + L + GA I+ K N G T LH+    G   + + L++ G ++ E      T L  A+Q+GH      L+  GA+     +  +TP  +A +  ++ +   L
Sbjct:  279 LLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFM-----GAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGT-PVDIEGKNQVTPLHVAAHYNNDKVAMLLLENG-ASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE-----NGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 6 Score: 145.591 bits (366), Expect = 9.114e-35
Identity = 105/349 (30.09%), Postives = 167/349 (47.85%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAA---AFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            + R N  + +  LI NG  ++       T L +A++ G T I  LLL +GAN     +D +S L +A   G  EV  +LL    D       G T L  A+  G  ++V LLL  G+ ++ +     TPL +AA Y  D V + LLL NGA       +G + + +AA         ++I   LL++  + N     G   L ++A EG K +  +L+EN +D+     + L A    A   +  V ++L   GA I+    +G++ L ++   G   ++  L+  G D   +     TPLH A+ +G   CV  LL++GA PNE    G+ PL ++ + GY+ + E L
Sbjct:  465 AARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAM-LLLENGASAKAAAKNGYTPLHIAA-----KKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 7 Score: 104.375 bits (259), Expect = 3.753e-22
Identity = 104/441 (23.58%), Postives = 195/441 (44.22%), Query Frame = 2
Query: 2030 SEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +EG    + A    D   V  LL  G  I+  + +G     + +L L S   + ++++ L++    ++    +G   L IA+  G  L+  IL+EN A++++      +PL  AA   + EVV+ LL  GA      + GF+ L+++  QG+ ++++ LL    +   +       LH+A+ +       LLL++   P+     G  PL ++   G+  + + L++    +VN+++    + L +A           LLSRGA    +  +  TPLH +          +L+          I+    +G   +H+A+     +  ++L+   A +D    +  T LH+ A  G  ++ +LL+D   + +     G T L IA ++  +++  LL+ + A +        TP  VA     I I   L + GA  D + V
Sbjct:   31 AEGSASFLRAARAGDLEKVLELLRAGTDINTSNANG-----LNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLL----ENDSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKG-ANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAP-----ISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETV 456          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Match: CG10011 (gene:FBgn0039590 transcript:FBtr0085319)

HSP 1 Score: 439.113 bits (1128), Expect = 2.633e-129
Identity = 239/553 (43.22%), Postives = 349/553 (63.11%), Query Frame = 2
Query: 1712 LDINCVNENGTTSLMVAAQNGFTSICNLLLS------SGANQLM------IDKDGWSALRLAIWNGYIEVVKLLLSQ---KIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKH 3316
            +D+   + NG T+L +AA+NG   +  LLLS       G  ++        D+DGW+ LR A W G+ EVV+LL++Q   KID      +GRTALR AAW G  DI+ LL+ SG+++N  D +GRT L+AA+Y   Y +V++LL NGA ++H D DGRSA+ VAALC   +S Y  +I  LL +  N + +DN+GM+ LL++++EG+  +C++LLEN AD D+ D   R+PL AA  +G+  VV+LLLFWG  I C+D  G +VLSI A+QGN + + +LL RGLDE HRDN G TPLH A+ EG +E    LL+SGAK +E DN G+  L L+ QEG +   + L+D+    V+ K++DG T+ ++A L    + VEFLL    D   +D + RT L+I  L+++ +  + LL  +   N+ ++N  D +GRTA+H+A+W+G  +++K+LI+ GA ++  D E RT LH  AWQG + +  +L+ +G   D  C QGATAL I++QEGH +    L+ FGAN    D C RTP K+A K +   I  + E +
Sbjct: 1262 IDLEIEDYNGQTALNIAARNGHLEVVKLLLSFSQPCNDGTGRMKRVDVNHADRDGWTPLRSASWGGHSEVVRLLIAQPACKID--LADKEGRTALRAAAWSGHEDILKLLIESGADVNSVDRQGRTSLIAASYMGHYDIVEILLENGANVNHLDLDGRSALCVAALC--GSSGYSKVISTLLDHGANTDQLDNDGMSPLLVSSFEGNAEVCELLLENAADPDLADFMGRTPLWAACTAGHATVVKLLLFWGCGIDCMDSEGRTVLSIGAAQGNVETVRQLLDRGLDETHRDNAGWTPLHYAAFEGFHEVCLQLLESGAKIDECDNEGKTALHLAAQEGRLHCVQALLDIHSSFVDQKAHDGKTAFRLACLEGHMDTVEFLLKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMT---NV-DVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEARTPLHSCAWQGNHDVMNILLYYGALADHACKQGATALGISAQEGHEKCVIALLQFGANPYKSDHCGRTPIKLAAKSSRTSILKIFESY 1806          

HSP 2 Score: 206.453 bits (524), Expect = 2.259e-53
Identity = 163/546 (29.85%), Postives = 264/546 (48.35%), Query Frame = 2
Query: 1829 DKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTG--NDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISA---SQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYD-CFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGAR-IDLKDNEGRTGLHLGAWQGCYQICQLLIDF----------------------------------GINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTP-YKVAYKGNHIKICNLLEKHGALVD 3331
            D +G +AL +A  NG++EVVKLLLS      F+   NDG          GR   V        ++N  D +G TPL +A++     +V+LL+   A                                     C I+L D EG   L  AA+ G + + K+L+E+ AD++ VDR   + L+AA++ G++++V +LL  GA ++ +D  G S L ++A   S G S+++S LL  G +    DN G +PL ++S EG  E   LLL++ A P+  D  GR PL  +C  G+  +  KL+  W   ++    +G T L I       E V  LL RG D T +DN G TPLH +  +  ++ C Q+L   +       I+  D++G+TA+H+A+ +G    +++L+D+ +  +D K ++G+T   L   +G     + L+ F                                   ++++   S+G TAL +A+ +GH ++   LI  GA+V  +D   RTP +  A++GNH  + N+L  +GAL D
Sbjct: 1268 DYNGQTALNIAARNGHLEVVKLLLS------FSQPCNDG---------TGRMKRV--------DVNHADRDGWTPLRSASWGGHSEVVRLLIAQPA-------------------------------------CKIDLADKEGRTALRAAAWSGHEDILKLLIESGADVNSVDRQGRTSLIAASYMGHYDIVEILLENGANVNHLDLDGRSALCVAALCGSSGYSKVISTLLDHGANTDQLDNDGMSPLLVSSFEGNAEVCELLLENAADPDLADFMGRTPLWAACTAGHATVV-KLLLFWGCGIDCMDSEGRTVLSIGAAQGNVETVRQLLDRGLDETHRDNAGWTPLHYAAFEGFHEVCLQLLESGA------KIDECDNEGKTALHLAAQEGRLHCVQALLDIHSSFVDQKAHDGKTAFRLACLEGHMDTVEFLLKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEARTPLHSCAWQGNH-DVMNILLYYGALAD 1745          

HSP 3 Score: 203.756 bits (517), Expect = 1.690e-52
Identity = 121/320 (37.81%), Postives = 181/320 (56.56%), Query Frame = 2
Query:  425 NLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSD-YLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFR--HNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASA 1375
            N+   PD  FK   L PLL   PP   + +LIDSIDEN +++  L   L       T HK+R    N+++LLS H + FP+WL LVCT +K+ + I + F GF+K  LDDL KS+V++D+Q YI+NR++ D     M+  E  E L+ L+IKSNG I+YLE VL GI D + S + IK IP  L GLYL+I +  F   Q ++ ++ + N++L+S   +    ++  L   +   D    +  L  ++  L     N    +  HNS  +WL+DVK  T K+ C + +GHV +++Y   ++  L    +R FA+HLI S 
Sbjct:  744 NIEKNPDECFKKAFLFPLLELTPPKTALLLLIDSIDENYINEGNLISTLKGGRGTVTNHKSR----NVAELLSNHIHLFPKWLFLVCTTKKQTKQITKMFTGFKKITLDDLRKSHVVKDVQEYIINRLNSDFKDSIMLTKEIIESLHQLYIKSNGCILYLEKVLHGIKDNFFSFREIKLIPCTLNGLYLYICQKSFNKKQ-YMKIRPLLNVLLASSGYVDKLFLFNCLRTHNYTIDCQEFEKRLQLMRNILAYDS-NAQRLKIFHNSFADWLVDVKFATKKFICDVNEGHVMISMYYLLVADTLCANTVRRFAYHLIRSG 1057          

HSP 4 Score: 162.925 bits (411), Expect = 5.776e-40
Identity = 144/483 (29.81%), Postives = 222/483 (45.96%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIW---NGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN 3127
            LI +G D+N V+  G TSL+ A+  G   I  +LL +GAN   +D DG SAL +A     +GY +V+  LL    + D   NDG + L  +++ G  ++  LLL + ++ +  D  GRTPL AA       +VKLLL  G  ID  D++GR+ + + A   N     ++ ++ LL    +    DN G   L  AA+EG   +C  LLE+ A ID  D   ++ L  AA  G              +HC+                 Q L ++ +  +D+   D  G T   LA +EG  + V  LLK     N  D   R  L +   E  ++I + L+D+    VN    +G T+L +A      + V+ L+  GAD    D   RTPLH    Q  +D   +LL +     + +  C    G TA+ I++ +G  + + +L+  GA     D+ GRT + L A      I ++   +  N
Sbjct: 1369 LIESGADVNSVDRQGRTSLIAASYMGHYDIVEILLENGANVNHLDLDGRSALCVAALCGSSGYSKVISTLLDHGANTDQLDNDGMSPLLVSSFEGNAEVCELLLENAADPDLADFMGRTPLWAACTAGHATVVKLLLFWGCGIDCMDSEGRTVLSIGAAQGN-----VETVRQLLDRGLDETHRDNAGWTPLHYAAFEGFHEVCLQLLESGAKIDECDNEGKTALHLAAQEGR-------------LHCV-----------------QALLDIHSSFVDQKAHD--GKTAFRLACLEGHMDTVEFLLKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEARTPLHSCAWQGNHDVMNILLYYGA---LADHAC--KQGATALGISAQEGHEKCVIALLQFGANPYKSDHCGRTPIKLAAKSSRTSILKIFESYTKN 1809          

HSP 5 Score: 148.673 bits (374), Expect = 1.505e-35
Identity = 119/408 (29.17%), Postives = 202/408 (49.51%), Query Frame = 2
Query: 1499 DIVKILFESINLVNINKLNAKKSFTI--------FSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLN-NGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLE-NNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKI 2683
            DIV+IL E+   VN   L+ + +  +        +S  I  +    +N+ +  N     L  S  + +      L+ N  D +  +  G T L  A   G  ++  LLL  G     +D +G + L +    G +E V+ LL + +D     N G T L  AA+ G  ++ L LL SG+ I++ D+EG+T L  A  +     V+ LL+ + +++D + +DG++A  +A  CL     ++D ++ LL++ C++N  D +    L I A E    + K LL+  N D++I D   R+ L  AA+ G+ ++V+ L+  GA ++ +D    + L   A QGN  +++ LL  G    H    G T L +++ EG  +CV  LL+ GA P + D+ GR P+ L+ +     I
Sbjct: 1397 DIVEILLENGANVNHLDLDGRSALCVAALCGSSGYSKVISTLLDHGANTDQLDNDGMSPLLVSSFEGNAEVCELLLENAADPDLADFMGRTPLWAACTAGHATVVKLLLFWGCGIDCMDSEGRTVLSIGAAQGNVETVRQLLDRGLDETHRDNAGWTPLHYAAFEGFHEVCLQLLESGAKIDECDNEGKTALHLAAQEGRLHCVQALLDIHSSFVDQKAHDGKTAFRLA--CLEG---HMDTVEFLLKFCCDVNSKDADSRTTLYILALENKLEIVKYLLDMTNVDVNIPDSEGRTALHVAAWQGHADMVKTLIEAGADVNSMDLEARTPLHSCAWQGNHDVMNILLYYGALADHACKQGATALGISAQEGHEKCVIALLQFGANPYKSDHCGRTPIKLAAKSSRTSI 1799          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303119)

HSP 1 Score: 218.394 bits (555), Expect = 1.464e-57
Identity = 165/564 (29.26%), Postives = 270/564 (47.87%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  +   + + + N +DIN  N NG  +L +A+++G   + + LL  GA      K G +AL +A   G  EVVKLLL      +    +G T L  AA      +V LLLS+G+N +    +G TPL  A  +    +V +LL         ++D R  + + AL + +    +    LLL  + N ++    G   L IA++ G++ +  +L++  AD++   +   SPL  AA  G   +V LLL  G  I    + G + L  +A  G+ Q++  LL RG     +   G  PLH+A+     +   +LL   A  +E+       L ++   G++++A+ L+D   ++ N ++ +G T L IA      + VE LL  GA  +    +G TPLH++      +    LLQH  + ++  +      G T +H+A+     +II+ L+  GA++D +  E +T LH+ +  G   I  LL+  G  +D T     TAL IA++EG  E+  +LI  GA +        TP  +  K  HIK+  LL +  A VD
Sbjct:   14 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL---------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADA-NARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR-----GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVD 562          

HSP 2 Score: 203.756 bits (517), Expect = 5.763e-53
Identity = 159/550 (28.91%), Postives = 259/550 (47.09%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAAL--CLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            N +S L+  G +I     +G T L  AA++G   + ++LL  GA      K+G + L +A    +++  ++LL  +   D    D  TAL  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL +GA I      G + + VAA   C+N       I+  LL+++ + ++    G   L +AA      + +ILL N A +D   R   +PL  A+  GN ++V LLL  GA +    K  ++ L I+A +G  ++ + L+  G  LD   +  F  TPLHL +  G  +   LLL+  A  +    +G  PL ++C     ++A  L++    S +  + +G T L IA      +    LL  GA A  +   G TPLH+S  +   +   +L++H        +N    +G T +H+ + + +  + + L   GA ID+    G T LH+ +  G   + + L+  G N+D   S G T L   +Q+GH  I NLL+   AN        +TP  +A K  +I + + L+
Sbjct:  252 NMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMN-------IVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF--TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKG-ASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAA-----VNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLK 786          

HSP 3 Score: 203.371 bits (516), Expect = 7.615e-53
Identity = 169/633 (26.70%), Postives = 282/633 (44.55%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALR---------------------------------TAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIGFSSYYILNSF 3400
            L+ +   +N  ++NG T L +AAQ    ++  LLLS+GANQ +  +DG++ L +A+  G+ +VV +LL                ++K D             PD T   G T L                                   AA  G+T++V LLL  G NI  +  +G TPL  A       +V +LL  GA I  +  +G     +A L + +  +++D  ++LL +   ++ +  + +  L +AA+ G   + K+LL+ NAD +   +   +PL  A      +VV LLL  GA+I    +SG + L ++A  G   I+  LL            G TPLHLA+   Q + + +LL++GA+ +      + PL ++ + G + I   L+    + V+  + D  T+L IA      E    L+  GA        G TPLH++         Q+LLQ        +++    +G T +H+A    + ++   L++ GA        G T LH+ A +    I   L+++G   +     G T L ++SQEGH EI NLLI   A V H      TP  +  + +++ +  +LEK+GA +D            G++  ++ + F
Sbjct:   96 LLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNG-----LAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQ-VDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA-----DVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANID-------MATKAGYTPLHVASHF 710          

HSP 4 Score: 200.29 bits (508), Expect = 7.320e-52
Identity = 176/627 (28.07%), Postives = 291/627 (46.41%), Query Frame = 2
Query: 1496 DDIVKILFESINLVNIN----KLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIIN-GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            D +V +L ES     +      + AKK     +T +L+      ++ + T+   F+            I+ L+I  G D+N   ++  + L VAA+ G T++ +LLL  G N     +DG + L  A  +G+ +VV +LL +         +G   L  AA     D   +LL   + +++   +  T L  A + C +V V KLLL+  A     D + R+      L +      L +++LLLR+  +I+     G+  L +AA+ G   +   LL+++A  D+      +PL  AA +   +++R+LL  GA +    +   + L I++  GN  I+  LL  G  +D   +D +  T LH+A+ EGQ E   +L+++GA  +     G  PL L+ + G+IK+A+ L+    + V+ +  +G T L +A      +    LL +GA       NG TPLHI+  ++Q D    LL++         N     G T +H++S +G  EI   LI+  A ++     G T +HL A +    + ++L   G NID     G T L +AS  G   +   L+  GANV    S   TP     +  H  I NLL +H A  + + V
Sbjct:  156 DKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLD----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAH-CGHVRVAKLLLDRNA-----DANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTV 764          

HSP 5 Score: 125.561 bits (314), Expect = 1.231e-28
Identity = 97/354 (27.40%), Postives = 158/354 (44.63%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPE 2713
            + R N  + I  L+ NG  ++       T L +A++ G   I  LLL  GA      KD ++AL +A   G  EV  +L+      D     G T L   A  G   +  LLL   ++++ Q   G TPL  A +  +  +  LLL  GA       +G + + +AA         +DI   LL Y    N     G   L +++ EG   +  +L+E+ A ++   +   +P+   A   N  V  +L   GA I    K+G++ L +++  G + ++  LL  G +     + G TPLH  + +G    VNLLL+  A  N    +G+ PL ++ + GYI + + L  +  E
Sbjct:  443 AARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAA-----RKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKE 791          

HSP 6 Score: 115.546 bits (288), Expect = 1.515e-25
Identity = 99/348 (28.45%), Postives = 156/348 (44.83%), Query Frame = 2
Query: 2303 ILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            ++ EN A  D    +  + AA +GN E V   L     I+  + +G + L +++  G+  ++SELL RG         G T LH+AS+ GQ E V LLL+  A  N    +G  PL ++ QE +  +   L+       +  + DG T L +A+     + V  LL       +     R P LHI+  +       +LL      N +N +     G T +HIAS  G+  I   LI  GA ++       + LH+ A  G   +  LL++ G NI+     G T L  A++ GH ++ ++L+  GA +         P  +A +G H+    +L  H A VD   V
Sbjct:    1 MVTENGAQGD--GNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQ-SLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKV-----RLPALHIAAKKDDVKAATLLLD-----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTV 335          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334061)

HSP 1 Score: 217.238 bits (552), Expect = 4.734e-57
Identity = 169/568 (29.75%), Postives = 276/568 (48.59%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMR---TPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  +   + + + N +DIN  N NG  +L +A+++G   + + LL  GA      K G +AL +A   G  EVVKLLL      +    +G T L  AA      +V LLLS+G+N +    +G TPL  A  +    +V +LL         ++D R  + + AL + +    +    LLL  + N ++    G   L IA++ G++ +  +L++  AD++   +   SPL  AA  G   +V LLL  G  I    + G + L  +A  G+ Q++  LL RG     +   G  PLH+A+     +   +LL   A  +E+       L ++   G++++A+ L+D   ++ N ++ +G T L IA   NRL + VE LL  GA  +    +G TPLH++      +    LLQH  + ++  +      G T +H+A+     +II+ L+  GA++D +  E +T LH+ +  G   I  LL+  G  +D T     TAL IA++EG  E+  +LI  GA    +D+  +   TP  +  K  HIK+  LL +  A VD
Sbjct:  164 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL---------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADA-NARALNGFTPLHIACKKNRL-KVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR-----GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAA---LDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVD 712          

HSP 2 Score: 203.756 bits (517), Expect = 8.610e-53
Identity = 159/550 (28.91%), Postives = 259/550 (47.09%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAAL--CLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            N +S L+  G +I     +G T L  AA++G   + ++LL  GA      K+G + L +A    +++  ++LL  +   D    D  TAL  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL +GA I      G + + VAA   C+N       I+  LL+++ + ++    G   L +AA      + +ILL N A +D   R   +PL  A+  GN ++V LLL  GA +    K  ++ L I+A +G  ++ + L+  G  LD   +  F  TPLHL +  G  +   LLL+  A  +    +G  PL ++C     ++A  L++    S +  + +G T L IA      +    LL  GA A  +   G TPLH+S  +   +   +L++H        +N    +G T +H+ + + +  + + L   GA ID+    G T LH+ +  G   + + L+  G N+D   S G T L   +Q+GH  I NLL+   AN        +TP  +A K  +I + + L+
Sbjct:  402 NMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMN-------IVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF--TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKG-ASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAA-----VNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLK 936          

HSP 3 Score: 202.986 bits (515), Expect = 1.386e-52
Identity = 169/633 (26.70%), Postives = 282/633 (44.55%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALR---------------------------------TAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIGFSSYYILNSF 3400
            L+ +   +N  ++NG T L +AAQ    ++  LLLS+GANQ +  +DG++ L +A+  G+ +VV +LL                ++K D             PD T   G T L                                   AA  G+T++V LLL  G NI  +  +G TPL  A       +V +LL  GA I  +  +G     +A L + +  +++D  ++LL +   ++ +  + +  L +AA+ G   + K+LL+ NAD +   +   +PL  A      +VV LLL  GA+I    +SG + L ++A  G   I+  LL            G TPLHLA+   Q + + +LL++GA+ +      + PL ++ + G + I   L+    + V+  + D  T+L IA      E    L+  GA        G TPLH++         Q+LLQ        +++    +G T +H+A    + ++   L++ GA        G T LH+ A +    I   L+++G   +     G T L ++SQEGH EI NLLI   A V H      TP  +  + +++ +  +LEK+GA +D            G++  ++ + F
Sbjct:  246 LLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNG-----LAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQ-VDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA-----DVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANID-------MATKAGYTPLHVASHF 860          

HSP 4 Score: 200.675 bits (509), Expect = 9.138e-52
Identity = 176/627 (28.07%), Postives = 291/627 (46.41%), Query Frame = 2
Query: 1496 DDIVKILFESINLVNIN----KLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIIN-GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            D +V +L ES     +      + AKK     +T +L+      ++ + T+   F+            I+ L+I  G D+N   ++  + L VAA+ G T++ +LLL  G N     +DG + L  A  +G+ +VV +LL +         +G   L  AA     D   +LL   + +++   +  T L  A + C +V V KLLL+  A     D + R+      L +      L +++LLLR+  +I+     G+  L +AA+ G   +   LL+++A  D+      +PL  AA +   +++R+LL  GA +    +   + L I++  GN  I+  LL  G  +D   +D +  T LH+A+ EGQ E   +L+++GA  +     G  PL L+ + G+IK+A+ L+    + V+ +  +G T L +A      +    LL +GA       NG TPLHI+  ++Q D    LL++         N     G T +H++S +G  EI   LI+  A ++     G T +HL A +    + ++L   G NID     G T L +AS  G   +   L+  GANV    S   TP     +  H  I NLL +H A  + + V
Sbjct:  306 DKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLD----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAH-CGHVRVAKLLLDRNA-----DANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGAL-----ANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTV 914          

HSP 5 Score: 126.331 bits (316), Expect = 8.728e-29
Identity = 97/354 (27.40%), Postives = 158/354 (44.63%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPE 2713
            + R N  + I  L+ NG  ++       T L +A++ G   I  LLL  GA      KD ++AL +A   G  EV  +L+      D     G T L   A  G   +  LLL   ++++ Q   G TPL  A +  +  +  LLL  GA       +G + + +AA         +DI   LL Y    N     G   L +++ EG   +  +L+E+ A ++   +   +P+   A   N  V  +L   GA I    K+G++ L +++  G + ++  LL  G +     + G TPLH  + +G    VNLLL+  A  N    +G+ PL ++ + GYI + + L  +  E
Sbjct:  593 AARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAA-----RKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKE 941          

HSP 6 Score: 112.464 bits (280), Expect = 1.427e-24
Identity = 95/332 (28.61%), Postives = 150/332 (45.18%), Query Frame = 2
Query: 2351 LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
             + AA +GN E V   L     I+  + +G + L +++  G+  ++SELL RG         G T LH+AS+ GQ E V LLL+  A  N    +G  PL ++ QE +  +   L+     + +  + DG T L +A+     + V  LL       +     R P LHI+  +       +LL      N +N +     G T +HIAS  G+  I   LI  GA ++       + LH+ A  G   +  LL++ G NI+     G T L  A++ GH ++ ++L+  GA +         P  +A +G H+    +L  H A VD   V
Sbjct:  165 FLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNG-ANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKV-----RLPALHIAAKKDDVKAATLLLD-----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTV 485          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303117)

HSP 1 Score: 217.238 bits (552), Expect = 1.410e-56
Identity = 165/564 (29.26%), Postives = 270/564 (47.87%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  +   + + + N +DIN  N NG  +L +A+++G   + + LL  GA      K G +AL +A   G  EVVKLLL      +    +G T L  AA      +V LLLS+G+N +    +G TPL  A  +    +V +LL         ++D R  + + AL + +    +    LLL  + N ++    G   L IA++ G++ +  +L++  AD++   +   SPL  AA  G   +V LLL  G  I    + G + L  +A  G+ Q++  LL RG     +   G  PLH+A+     +   +LL   A  +E+       L ++   G++++A+ L+D   ++ N ++ +G T L IA      + VE LL  GA  +    +G TPLH++      +    LLQH  + ++  +      G T +H+A+     +II+ L+  GA++D +  E +T LH+ +  G   I  LL+  G  +D T     TAL IA++EG  E+  +LI  GA +        TP  +  K  HIK+  LL +  A VD
Sbjct:   14 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL---------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADA-NARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR-----GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVD 562          

HSP 2 Score: 200.675 bits (509), Expect = 2.100e-51
Identity = 159/550 (28.91%), Postives = 259/550 (47.09%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAAL--CLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            N +S L+  G +I     +G T L  AA++G   + ++LL  GA      K+G + L +A    +++  ++LL  +   D    D  TAL  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL +GA I      G + + VAA   C+N       I+  LL+++ + ++    G   L +AA      + +ILL N A +D   R   +PL  A+  GN ++V LLL  GA +    K  ++ L I+A +G  ++ + L+  G  LD   +  F  TPLHL +  G  +   LLL+  A  +    +G  PL ++C     ++A  L++    S +  + +G T L IA      +    LL  GA A  +   G TPLH+S  +   +   +L++H        +N    +G T +H+ + + +  + + L   GA ID+    G T LH+ +  G   + + L+  G N+D   S G T L   +Q+GH  I NLL+   AN        +TP  +A K  +I + + L+
Sbjct:  252 NMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMN-------IVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF--TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEK-GASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAA-----VNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLK 786          

HSP 3 Score: 200.29 bits (508), Expect = 2.693e-51
Identity = 163/605 (26.94%), Postives = 280/605 (46.28%), Query Frame = 2
Query: 1613 SNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSG--------------------ANQLMIDKD---------GWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S ++++ N ++  L+ + ++N    +  L+ NG + +   E+G T L VA Q G   +  +LL S                     A  L++D D         G++ L +A   G   +  LL+ +  D +++     + L  AA  G+T++V LLL  G NI  +  +G TPL  A       +V +LL  GA I  +  +G + + +AA       +++D  ++LL +   ++ +  + +  L +AA+ G   + K+LL+ NAD +   +   +PL  A      +VV LLL  GA+I    +SG + L ++A  G   I+  LL            G TPLHLA+   Q + + +LL++GA+ +      + PL ++ + G + I   L+    + V+  + D  T+L IA      E    L+  GA        G TPLH++         Q+LLQ        +++    +G T +H+A    + ++   L++ GA        G T LH+ A +    I   L+++G   +     G T L ++SQEGH EI NLLI   A V H      TP  +  + +++ +  +LEK+GA +D
Sbjct:  102 SVNVQSQNGFT-PLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAA-----QGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQ-VDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA-----DVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANID 694          

HSP 4 Score: 198.749 bits (504), Expect = 7.397e-51
Identity = 167/598 (27.93%), Postives = 279/598 (46.66%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALC-------------LNSNSKYLD---------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +L  + +++     + L+ N  + +  +++G T L +A+  G  +I NLL+  GA+     K   S L +A   G   +V LLL +  + +    DG T L  AA  G   +V +LL  G+ I+ +   G  PL  A         ++LL + A +D    D  +A+ VAA C              ++N++ L+               +++LLLR+  +I+     G+  L +AA+ G   +   LL+++A  D+      +PL  AA +   +++R+LL  GA +    +   + L I++  GN  I+  LL  G  +D   +D +  T LH+A+ EGQ E   +L+++GA  +     G  PL L+ + G+IK+A+ L+    + V+ +  +G T L +A      +    LL +GA       NG TPLHI+  ++Q D    LL++    N  +       G T +H++S +G  EI   LI+  A ++     G T +HL A +    + ++L   G NID     G T L +AS  G   +   L+  GANV    S   TP     +  H  I NLL +H A  + + V
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAES-----KAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTV 764          

HSP 5 Score: 123.25 bits (308), Expect = 9.763e-28
Identity = 97/352 (27.56%), Postives = 157/352 (44.60%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPE 2713
            R N  + I  L+ NG  ++       T L +A++ G   I  LLL  GA      KD ++AL +A   G  EV  +L+      D     G T L   A  G   +  LLL   ++++ Q   G TPL  A +  +  +  LLL  GA       +G + + +AA         +DI   LL Y    N     G   L +++ EG   +  +L+E+ A ++   +   +P+   A   N  V  +L   GA I    K+G++ L +++  G + ++  LL  G +     + G TPLH  + +G    VNLLL+  A  N    +G+ PL ++ + GYI + + L  +  E
Sbjct:  445 RANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAA-----RKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKE 791          

HSP 6 Score: 116.316 bits (290), Expect = 1.379e-25
Identity = 99/348 (28.45%), Postives = 156/348 (44.83%), Query Frame = 2
Query: 2303 ILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            ++ EN A  D    +  + AA +GN E V   L     I+  + +G + L +++  G+  ++SELL RG         G T LH+AS+ GQ E V LLL+  A  N    +G  PL ++ QE +  +   L+       +  + DG T L +A+     + V  LL       +     R P LHI+  +       +LL      N +N +     G T +HIAS  G+  I   LI  GA ++       + LH+ A  G   +  LL++ G NI+     G T L  A++ GH ++ ++L+  GA +         P  +A +G H+    +L  H A VD   V
Sbjct:    1 MVTENGAQGD--GNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQ-SLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKV-----RLPALHIAAKKDDVKAATLLLD-----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTV 335          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303121)

HSP 1 Score: 217.238 bits (552), Expect = 1.413e-56
Identity = 165/564 (29.26%), Postives = 270/564 (47.87%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  +   + + + N +DIN  N NG  +L +A+++G   + + LL  GA      K G +AL +A   G  EVVKLLL      +    +G T L  AA      +V LLLS+G+N +    +G TPL  A  +    +V +LL         ++D R  + + AL + +    +    LLL  + N ++    G   L IA++ G++ +  +L++  AD++   +   SPL  AA  G   +V LLL  G  I    + G + L  +A  G+ Q++  LL RG     +   G  PLH+A+     +   +LL   A  +E+       L ++   G++++A+ L+D   ++ N ++ +G T L IA      + VE LL  GA  +    +G TPLH++      +    LLQH  + ++  +      G T +H+A+     +II+ L+  GA++D +  E +T LH+ +  G   I  LL+  G  +D T     TAL IA++EG  E+  +LI  GA +        TP  +  K  HIK+  LL +  A VD
Sbjct:   14 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLL---------ESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADA-NARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVR-----GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVD 562          

HSP 2 Score: 200.675 bits (509), Expect = 2.104e-51
Identity = 159/550 (28.91%), Postives = 259/550 (47.09%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAAL--CLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            N +S L+  G +I     +G T L  AA++G   + ++LL  GA      K+G + L +A    +++  ++LL  +   D    D  TAL  AA  G   +  LLL   ++ N +   G TPL  A  K    +V+LLL +GA I      G + + VAA   C+N       I+  LL+++ + ++    G   L +AA      + +ILL N A +D   R   +PL  A+  GN ++V LLL  GA +    K  ++ L I+A +G  ++ + L+  G  LD   +  F  TPLHL +  G  +   LLL+  A  +    +G  PL ++C     ++A  L++    S +  + +G T L IA      +    LL  GA A  +   G TPLH+S  +   +   +L++H        +N    +G T +H+ + + +  + + L   GA ID+    G T LH+ +  G   + + L+  G N+D   S G T L   +Q+GH  I NLL+   AN        +TP  +A K  +I + + L+
Sbjct:  252 NMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMN-------IVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGF--TPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEK-GASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAA-----VNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLK 786          

HSP 3 Score: 200.29 bits (508), Expect = 2.698e-51
Identity = 163/605 (26.94%), Postives = 280/605 (46.28%), Query Frame = 2
Query: 1613 SNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSG--------------------ANQLMIDKD---------GWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S ++++ N ++  L+ + ++N    +  L+ NG + +   E+G T L VA Q G   +  +LL S                     A  L++D D         G++ L +A   G   +  LL+ +  D +++     + L  AA  G+T++V LLL  G NI  +  +G TPL  A       +V +LL  GA I  +  +G + + +AA       +++D  ++LL +   ++ +  + +  L +AA+ G   + K+LL+ NAD +   +   +PL  A      +VV LLL  GA+I    +SG + L ++A  G   I+  LL            G TPLHLA+   Q + + +LL++GA+ +      + PL ++ + G + I   L+    + V+  + D  T+L IA      E    L+  GA        G TPLH++         Q+LLQ        +++    +G T +H+A    + ++   L++ GA        G T LH+ A +    I   L+++G   +     G T L ++SQEGH EI NLLI   A V H      TP  +  + +++ +  +LEK+GA +D
Sbjct:  102 SVNVQSQNGFT-PLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAA-----QGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQ-VDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEA-----DVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANID 694          

HSP 4 Score: 198.749 bits (504), Expect = 7.412e-51
Identity = 167/598 (27.93%), Postives = 279/598 (46.66%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALC-------------LNSNSKYLD---------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +L  + +++     + L+ N  + +  +++G T L +A+  G  +I NLL+  GA+     K   S L +A   G   +V LLL +  + +    DG T L  AA  G   +V +LL  G+ I+ +   G  PL  A         ++LL + A +D    D  +A+ VAA C              ++N++ L+               +++LLLR+  +I+     G+  L +AA+ G   +   LL+++A  D+      +PL  AA +   +++R+LL  GA +    +   + L I++  GN  I+  LL  G  +D   +D +  T LH+A+ EGQ E   +L+++GA  +     G  PL L+ + G+IK+A+ L+    + V+ +  +G T L +A      +    LL +GA       NG TPLHI+  ++Q D    LL++    N  +       G T +H++S +G  EI   LI+  A ++     G T +HL A +    + ++L   G NID     G T L +AS  G   +   L+  GANV    S   TP     +  H  I NLL +H A  + + V
Sbjct:  175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMY--TALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEAD-VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAES-----KAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTV 764          

HSP 5 Score: 123.25 bits (308), Expect = 9.775e-28
Identity = 97/352 (27.56%), Postives = 157/352 (44.60%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPE 2713
            R N  + I  L+ NG  ++       T L +A++ G   I  LLL  GA      KD ++AL +A   G  EV  +L+      D     G T L   A  G   +  LLL   ++++ Q   G TPL  A +  +  +  LLL  GA       +G + + +AA         +DI   LL Y    N     G   L +++ EG   +  +L+E+ A ++   +   +P+   A   N  V  +L   GA I    K+G++ L +++  G + ++  LL  G +     + G TPLH  + +G    VNLLL+  A  N    +G+ PL ++ + GYI + + L  +  E
Sbjct:  445 RANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAA-----RKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTITKE 791          

HSP 6 Score: 116.316 bits (290), Expect = 1.381e-25
Identity = 99/348 (28.45%), Postives = 156/348 (44.83%), Query Frame = 2
Query: 2303 ILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            ++ EN A  D    +  + AA +GN E V   L     I+  + +G + L +++  G+  ++SELL RG         G T LH+AS+ GQ E V LLL+  A  N    +G  PL ++ QE +  +   L+       +  + DG T L +A+     + V  LL       +     R P LHI+  +       +LL      N +N +     G T +HIAS  G+  I   LI  GA ++       + LH+ A  G   +  LL++ G NI+     G T L  A++ GH ++ ++L+  GA +         P  +A +G H+    +L  H A VD   V
Sbjct:    1 MVTENGAQGD--GNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQ-SLATEDGFTPLAVAMQQGHDKVVAVLLESDTRGKV-----RLPALHIAAKKDDVKAATLLLD-----NDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTV 335          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Match: ANKRD50 (ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:HGNC:29223])

HSP 1 Score: 550.051 bits (1416), Expect = 7.538e-176
Identity = 316/838 (37.71%), Postives = 483/838 (57.64%), Query Frame = 2
Query:  854 LLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVN----------INKLNAKKSFTIFSTYILNITSTYSN--SIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+ +E+Y A+   ++   +   +  +  +   L+    +     H S  EWLLDVK CT KY C+  +GH  +A+     + +L PL+++ FA HLI S    + + ++ A+W++++          P +   S +     +++++L ++   V+             L  + S         ++  T SN  ++ A   YS +L           ++ LI    ++  V+ +G T+L +AA+ G T + N L+   AN    D DGW+ALR A W G+ EVV  LL      D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D + M  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+AS EG  +  + L++ G++  E+DN GRIPL+LS QEG+      L++     ++ + YDG  +L++A L    + VE LLS  AD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI+  A ++  DNE R+ L   AWQG  +I Q LID G  +D TC+QGATAL IA+QEGH+E+  +L+   A+  H D   RT  +VA KG H  I  LLEK+GA
Sbjct:    1 MLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTVRELYHAVWTKNMTLTMEDFQKKMDILAKLLIDGLGSTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMMAMSYTCRAKQLKPLEVQEFAHHLIKSN--LQIEPFNLALWMVWNGT--------PAKDSLSTSIPKEQEVLQLLVKAGAHVSNEDDHASCIVQQALEREDSIRTLLDNGASVNQTDSNGRTLLANAAYSGNL---------EVVNLLISRRANMELVDSHGQTALTLAARQGHTKVVNCLIGCDANINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHGAVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMASFEGHRQVCDALIEQGSRSTEVDNDGRIPLVLSAQEGHYDCVHILLEN-KSCIDQRGYDGRNALRVAALEGHRDIVELLLSHEADIDFKDADGRPTLYILALENQLAMAEYFLE-----NGANVEASDMEGRTALHVSCWQGHVEMVRLLINYHADVNSCDNEKRSALQSAAWQGHIKIVQTLIDNGTVVDHTCNQGATALGIAAQEGHIEVVQILLEHSADPNHADQFGRTAMRVAAKGGHTAIIKLLEKYGA 812          

HSP 2 Score: 250.366 bits (638), Expect = 8.912e-68
Identity = 172/523 (32.89%), Postives = 279/523 (53.35%), Query Frame = 2
Query: 1796 LLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD---DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            LL +GA+    D +G + L  A ++G +EVV LL+S++ + +   + G+TAL  AA  G T +V  L+   +NIN  D +G T L +A +     +V  LL  GA +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK   + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A      +  + L+ +G+ +T  DN+GR PL +S  +  YDC  +LL+        N +C+D    DGR A+ +A+ +G  +I++ L+   A ID KD +GR  L++ A +    + +  ++ G N++ +  +G TAL ++  +GH+E+  LLIN+ A+V   D+  R+  + A    HIKI   L  +G +VD
Sbjct:  241 LLDNGASVNQTDSNGRTLLANAAYSGNLEVVNLLISRRANMELVDSHGQTALTLAARQGHTKVVNCLIGCDANINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHGAVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMASFEGHRQVCDALIEQGSRSTEVDNDGRIPLVLSAQEGHYDCVHILLE--------NKSCIDQRGYDGRNALRVAALEGHRDIVELLLSHEADIDFKDADGRPTLYILALENQLAMAEYFLENGANVEASDMEGRTALHVSCWQGHVEMVRLLINYHADVNSCDNEKRSALQSAAWQGHIKIVQTLIDNGTVVD 749          

HSP 3 Score: 183.726 bits (465), Expect = 1.633e-46
Identity = 145/494 (29.35%), Postives = 241/494 (48.79%), Query Frame = 2
Query: 1886 VKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISA-----SQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            ++ LL      + T ++GRT L  AA+ G  ++V LL+S  +N+   DS G+T L  A  +    +V  L+   A I+H D+DG +A+  AA   +S     +++  LL     ++  D +    L  AA+ G + +   LL++ A+++  D   R+ L+AAA+ G+ E+V  LL  GA ++  D  G + LS++A     S+G+  ++S L+ RG +  H D    TPL +A+ EG  + V+LLL+ GA  +  DN+GR PL+ +   G+  +                                  V  LL  GA     D+ GRT L I+  Q   +  + LL   L  N       DD G T +H+AS++G  ++  +LI+ G+R    DN+GR  L L A +G Y    +L++    ID+    G  AL +A+ EGH +I  LL++  A++   D+  R    +    N + +     ++GA V+  ++
Sbjct:  238 IRTLLDNGASVNQTDSNGRTLLANAAYSGNLEVVNLLISRRANMELVDSHGQTALTLAARQGHTKVVNCLIGCDANINHTDHDGWTALRSAAWGGHS-----EVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHGAVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASV----------------------------------VNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHR-----DDAGWTPLHMASFEGHRQVCDALIEQGSRSTEVDNDGRIPLVLSAQEGHYDCVHILLENKSCIDQRGYDGRNALRVAALEGHRDIVELLLSHEADIDFKDADGRPTLYILALENQLAMAEYFLENGANVEASDM 687          

HSP 4 Score: 170.629 bits (431), Expect = 1.911e-42
Identity = 126/427 (29.51%), Postives = 222/427 (51.99%), Query Frame = 2
Query: 2081 MVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVL-----NRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +++LL+  GA++ ++D D  S I   AL      +  D I+ LL    ++N  D+ G  +L  AAY G+  +  +L+   A++++VD   ++ L  AA  G+ +VV  L+   A I+  D  G++ L  +A  G+S+++S LL  G      D    T L  A+  G  + V  LL+ GA+ N+ DN GR  L+ +   G+ +I E L+D   E VN +  DG T+L +A L           V  L+ RGA+    D +  TPL ++  +   D   +LL+        +++  D++GRT +  A+  G   ++ +L+  GA +D  D+EGRT L + + QG  ++ + L+D G++ +     G T L +AS EGH ++C+ LI  G+    +D+  R P  ++ +  H    ++L ++ + +D++
Sbjct:  206 VLQLLVKAGAHVSNED-DHASCIVQQAL------EREDSIRTLLDNGASVNQTDSNGRTLLANAAYSGNLEVVNLLISRRANMELVDSHGQTALTLAARQGHTKVVNCLIGCDANINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAE-VNHEDVDGRTALSVAALCVPASKGHGAVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGA-----DVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMASFEGHRQVCDALIEQGSRSTEVDNDGRIPLVLSAQEGHYDCVHILLENKSCIDQR 619          

HSP 5 Score: 107.457 bits (267), Expect = 5.834e-23
Identity = 88/296 (29.73%), Postives = 139/296 (46.96%), Query Frame = 2
Query: 2561 SVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQ-EGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINN------------------------------INCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIA-----SQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            S+  + E + LL+K+GA  +  D+H    +  + + E  I+    L+D    SVN    +G T L  A  +   E V  L+SR A+  + D++G+T L ++  Q        L+      NIN+                              ++C D D RTA+  A+W G  +I+ +L+  GA ++  DNEGRT L   A+ G  +I + L+D G  ++     G TALS+A     + +GH  + +LLI+ GA V H D    TP  VA    H+ + +LL + GA VD  +
Sbjct:  199 SIPKEQEVLQLLVKAGAHVSNEDDHASCIVQQALEREDSIRT---LLDNGA-SVNQTDSNGRTLLANAAYSGNLEVVNLLISRRANMELVDSHGQTALTLAARQGHTKVVNCLI--GCDANINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHGAVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTD 488          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Match: ankrd50l (ankyrin repeat domain 50-like [Source:ZFIN;Acc:ZDB-GENE-041008-117])

HSP 1 Score: 392.119 bits (1006), Expect = 5.654e-115
Identity = 222/566 (39.22%), Postives = 333/566 (58.83%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLL---------SSGANQLM--IDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS---KYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR------SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSG--AKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDF-GINIDETC-SQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            L+ +G D +  +  G T+L +AA+ G   +  +LL          S A+ L+   D +GW+ALR A W G+ E V+ LL    + D   +DGRTALR AAW G  +I+L LL  G+ ++  D EGRTPL+AA Y      V++LL+ GA ++  D DGR+A+ VAALC+ S++    + +++ LLL    +    D +GM  LL+A+YEG + + ++LLE  AD+D          +PL+AAA  G+   V  +LFWGAA+  ID  G + L ++A++G+ +++  LL RGLDE H+D+ G TPLH A+ EG      +L + G  A+  E+D  GR PL+L+ QEG+      L+D     ++ ++YDG ++L  A L    + VE L+ RGAD  ++D  GR  L++ VL+ + D   +L++         +   D +GRTA+H+A+W+GD E  + L+  GA  + +D +GR  LH  AW+G     +LL+   G+N+D  C  QGATALS+A+QEGH EI  +L+  GAN  H+D   RTP KVA K +H  I  LLE +GA
Sbjct:  582 LLCHGADPSLTDNQGQTALTLAAKQGHVGVLQVLLDWVQEQRSKGSAAHTLLEHADSEGWTALRSAAWGGHKEAVRRLLEAGAEVDGCDSDGRTALRAAAWGGHEEILLTLLGHGAEVDHSDREGRTPLIAAAYMGHKETVEILLDAGADLNLADGDGRTALSVAALCVPSSAGGRGHGEVVSLLLERGADPEHKDRDGMTPLLLASYEGHEEVVELLLEAGADVDESSGAHPSSITPLLAAAAMGHTRTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSIEVVRALLDRGLDENHKDDLGWTPLHAAACEGHKSVCAILTERGSMARVGELDVEGRSPLILAAQEGHCSTVRLLLDR-KSPIDHRAYDGHSALSAAALQGHRDIVELLMRRGADTDVRDAEGRPLLYLLVLEGRLDMATLLIEKGGV----PLESKDAEGRTALHVAAWRGDLEGTELLLKYGADPNARDLDGRPPLHSVAWRGHTAAGRLLLRAKGLNVDLACKQQGATALSVAAQEGHSEIVVMLLEKGANPDHVDRYGRTPVKVAGKQSHFTIVRLLESYGA 1142          

HSP 2 Score: 244.973 bits (624), Expect = 1.368e-65
Identity = 133/397 (33.50%), Postives = 219/397 (55.16%), Query Frame = 2
Query:  188 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLS-DYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASA 1375
            K+ +C  L    +  C K   L S  LA H+C   D  S+ + +F+  + + L +S   G  Y + I+S+ +  +    +    PD  FK  +L PLLS  PP  +++I++DS+D      + +GE  S   +  T       S +I++LL  H   FP W+ LVC+ R++N+ + + F GFRK  LDDL K   +RD+Q YIL R+D+D  L++ +  ET E+LNLLHIKS G  ++LE V+DG+ +G + ++ I+ IPG L GLYLW+ + LF   + F+ ++ + NI+L+S + L+ +++Y    + D    +   +  +  +    V   +      H+S  EWL DVK CT K+ C + DGH+++A+  +  +S L+  +    A HL+ S 
Sbjct:   68 KTALCTELVWPQSETC-KAAGLASRCLAWHYCQREDAGSVEVWRFVLALVEQLKESPLLGPNYVKVISSASVATVLEPLHCQRAPDETFKRAVLEPLLSLTPPAHSVFIVVDSVDSGCCGGNGVGEG-SISGSAGTP------SSSIAELLLKHIQLFPPWILLVCSARRQNKAVCKMFSGFRKLCLDDLRKPATVRDVQQYILRRLDQDGALRRQLTPETAEMLNLLHIKSGGCFLFLERVMDGVANGLVGLREIRDIPGTLNGLYLWLCQRLFPR-RLFVHIRPLLNILLASQQPLTAEDLYTIAKSRDCYLGIRDFQAQMGALASLFVDGPEGSKLLFHSSFAEWLTDVKYCTQKFLCCVKDGHLSMAMSLSLRASSLSTEETCQLAHHLLNSG 455          

HSP 3 Score: 191.815 bits (486), Expect = 6.259e-49
Identity = 148/493 (30.02%), Postives = 237/493 (48.07%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWI--------GRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNAD---IDIVDRSPLMAAAFSGNFEVVRLLLFWG--AAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFG 3121
              + +L+  G +++  + +G T+L  AA  G   I   LL  GA     D++G + L  A + G+ E V++LL    D +    DGRTAL  AA          G  ++V LLL  G++   +D +G TPL+ A+Y+    +V+LLL  GA +D  ++ G     +  L   +   +   +  +L +   ++ ID EG   L +AA +GS  + + LL+   D    D +  +PL AAA  G+  V  +L   G  A +  +D  G S L ++A +G+   +  LL R     HR   G + L  A+++G  + V LL++ GA  +  D  GR  L L   EG + +A  LI+     +  K  +G T+L +A      E  E LL  GAD   +D +GR PLH    +      ++LL+      +N        G TA+ +A+ +G  EI+  L++ GA  D  D  GRT + +   Q  + I +LL  +G
Sbjct:  654 EAVRRLLEAGAEVDGCDSDGRTALRAAAWGGHEEILLTLLGHGAEVDHSDREGRTPLIAAAYMGHKETVEILLDAGADLNLADGDGRTALSVAALCVPSSAGGRGHGEVVSLLLERGADPEHKDRDGMTPLLLASYEGHEEVVELLLEAGADVD--ESSGAHPSSITPLLAAAAMGHTRTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSIEVVRALLDRGLDENHKDDLGWTPLHAAACEGHKSVCAILTERGSMARVGELDVEGRSPLILAAQEGHCSTVRLLLDRKSPIDHRAYDGHSALSAAALQGHRDIVELLMRRGADTDVRDAEGRPLLYLLVLEGRLDMATLLIEKGGVPLESKDAEGRTALHVAAWRGDLEGTELLLKYGADPNARDLDGRPPLHSVAWRGHTAAGRLLLR---AKGLNVDLACKQQGATALSVAAQEGHSEIVVMLLEKGANPDHVDRYGRTPVKVAGKQSHFTIVRLLESYG 1141          

HSP 4 Score: 141.739 bits (356), Expect = 2.173e-33
Identity = 107/351 (30.48%), Postives = 166/351 (47.29%), Query Frame = 2
Query: 1697 LIINGLDIN---CVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGS--NINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNA----DIDIVDRSPLMAAAFSGNFEVVRLLLFWGA-----------AIHCI------------------------DKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKP 2617
            L+  G D++     + +  T L+ AA  G T   N +L  GA    ID +G +AL LA   G IEVV+ LL + +D +   + G T L  AA  G   +  +L   GS   + + D EGR+PL+ A  +     V+LLL+  + IDH+  DG SA+  AAL       + DI++LL+R   + ++ D EG  +L +   EG   +  +L+E         D   R+ L  AA+ G+ E   LLL +GA            +H +                         + G + LS++A +G+S+I+  LL +G +  H D +G TP+ +A  +  +  V LL   GAKP
Sbjct:  799 LLEAGADVDESSGAHPSSITPLLAAAAMGHTRTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSIEVVRALLDRGLDENHKDDLGWTPLHAAACEGHKSVCAILTERGSMARVGELDVEGRSPLILAAQEGHCSTVRLLLDRKSPIDHRAYDGHSALSAAAL-----QGHRDIVELLMRRGADTDVRDAEGRPLLYLLVLEGRLDMATLLIEKGGVPLESKDAEGRTALHVAAWRGDLEGTELLLKYGADPNARDLDGRPPLHSVAWRGHTAAGRLLLRAKGLNVDLACKQQGATALSVAAQEGHSEIVVMLLEKGANPDHVDRYGRTPVKVAGKQSHFTIVRLLESYGAKP 1144          

HSP 5 Score: 59.6918 bits (143), Expect = 2.727e-8
Identity = 51/152 (33.55%), Postives = 73/152 (48.03%), Query Frame = 2
Query: 2918 NINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN-----------IDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            ++N  + LD  GRT +  A+  G  ++   L+  GA   L DN+G+T L L A QG   + Q+L+D+              ++   S+G TAL  A+  GH E    L+  GA V   DS  RT  + A  G H +I   L  HGA VD  +
Sbjct:  554 SVNRTDPLD--GRTLLAAAAHAGLVDVAALLLCHGADPSLTDNQGQTALTLAAKQGHVGVLQVLLDWVQEQRSKGSAAHTLLEHADSEGWTALRSAAWGGHKEAVRRLLEAGAEVDGCDSDGRTALRAAAWGGHEEILLTLLGHGAEVDHSD 703          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 229.95 bits (585), Expect = 1.066e-60
Identity = 165/580 (28.45%), Postives = 280/580 (48.28%), Query Frame = 2
Query: 1601 TSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            T  +S   +  +  + S   + R  +   +   +  G+DIN  N+NG  +L +A++ G   +   LL  GAN     K G +AL +A   G  EVV+ L++   + +    +G T L  AA     D+V  LL + S+ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D + R   +PL  A+  GN  +V+LLL  G+ I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+  A  +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +  + L  H  + N  N+      G TA+H+A+  G  ++++ L+  GA++D+K  + +T LH+ +  G  +I Q L+  G   +   + G T L ++++EGH EI  LL+  G+++        TP  VA K   +++ NLL +  A  D
Sbjct:   29 TRKHSRDQKKKSDSNASYLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVR-----GETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPD 593          

HSP 2 Score: 209.149 bits (531), Expect = 4.002e-54
Identity = 158/550 (28.73%), Postives = 257/550 (46.73%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            ++ + N +  L+  G  I    ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL      D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA +      G + I VAA   + N     I+K L  +  + N  +  G   L +AA  G   + + LL+N A +DI    D++ L  A+  G  E+V+ LL  GA  +    SG++ L +SA +G+ +I + LL +G         G TPLH+A+  GQ E  NLLL+  A P+     G  PL ++      ++A  L+D    S +  + +G T L IA      E    LL  GA+       G +PLH++  +   D   +LL         N+N  + +G T +H+A+      + + L++ GA ID +   G T LH+    G  ++   L++     +     G T L  A+Q+GH  I N+L+ +GA+   +     T   +A +  +I + + L
Sbjct:  278 KRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHEN-----IVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQG-ASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQA-----NVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTL 816          

HSP 3 Score: 190.274 bits (482), Expect = 2.942e-48
Identity = 157/591 (26.57%), Postives = 272/591 (46.02%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALC-------------LNSNSKYLD---------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNAD---IDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID--VWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +L  + R++   + + L+ N  + +  +++G T L +AA  G  ++  LLL+ GA    + ++  + L +A   G   +VKLLL +    +    DG T L   A  G   +V +LL  G+ I  +   G +PL  AT       V+LLL + A +D   ND  +A+ VAA C              N N+K L+               +++LLL++  ++  +   G+  + +AA+ G + + K L  + A     ++   + L  AA +G  +VVR LL  GA +    K   + L I++  G  +I+ +LL +G         G TPLHL++ EG  E   LLL+ G+  +     G  PL ++ + G +++A  L+     P++       G T L +A           LL +GA       NG TPLHI+  ++Q +    LL++         N +   G + +H+A+ +G  +++  L+   A +++ +  G T LHL A      + ++L++ G  ID     G T L +A   G++++ N L+   A          TP   A +  H  I N+L ++GA
Sbjct:  206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAA---GKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGA-----ECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGA 788          

HSP 4 Score: 189.119 bits (479), Expect = 5.694e-48
Identity = 168/633 (26.54%), Postives = 290/633 (45.81%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAV-LNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            IN+ N N LNA     K+        +L + +   N   AT   + +L  +        + +L+ NG ++N  ++NG T L +AAQ     +   LL + ++Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ I+ +  DG + +   A      S +  ++++LL     I      G++ L +A  +G  L C ++LL++NA +D V    L A   AA  G+++V ++++   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V  L   GA PN  +  G   L ++ + G I +   L+    + V+ K+ D  T+L IA  L +L E V+ LL +GA       +G TPLH+S  +   +   +LL+       ++++     G T +H+A+  G  E+   L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  LEI   L+ +GA    +     +P  +A +   + + +LL
Sbjct:   68 INICNQNGLNALHLASKEGHVEVVAELLKLGA---NVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGA-----RSGHEQVVEMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAK-VDIKAKDDQTALHIASRLGKL-EIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQG-----SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLL 684          

HSP 5 Score: 161.77 bits (408), Expect = 1.645e-39
Identity = 137/496 (27.62%), Postives = 226/496 (45.56%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            A   G +E V   L   +D +    +G  AL  A+  G  ++V  LL  G+N++    +G T L  A+      +V+ L+ NGA ++ Q  +G + +++AA        +LD+++ LL  N + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D M R++   TPLH+AS  G    V LLL  G+K                                  +  K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LLQH+       ++ + +D  TA+H+A+  G  ++ K ++D  A  + K   G T LH+   +   ++ +LL+  G ++      G T + +A+  GH  I   L + GA+    +    T   +A +   I +   L ++GA VD K
Sbjct:   49 AARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAA-----QENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDI--TPLHVASKRGNGNMVKLLLDRGSK----------------------------------IEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAP-----VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIK 496          

HSP 6 Score: 157.147 bits (396), Expect = 4.965e-38
Identity = 132/468 (28.21%), Postives = 214/468 (45.73%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDV 3019
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +VK L      P+ T   G TAL  AA  G+ D+V  LL +G+ ++ +  + +T L  A+      +V+ LL  GA  +     G +      L L++   + +I  LLL    +++    +G   L +AA  G   +  +LL+  A  D   +S   PL  AA   N  V  LLL  GA+ H   K+G++ L I+A +   +I + LL  G +       G +PLHLA+ EG  + V+LLL   A  N  + +G  PL L+ Q+    + E L++   E ++ ++  G T L +A      +   FLL   A    +  NG TPLH +  Q       +LLQ+  + N   +N     G TA+ IA   G   ++ +L  V
Sbjct:  363 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYT-----PLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAE-IDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLN-----GNTALSIARRLGYISVVDTLRGV 819          

HSP 7 Score: 150.599 bits (379), Expect = 4.441e-36
Identity = 121/419 (28.88%), Postives = 189/419 (45.11%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRT 2845
            N + QL  +G   N  N  G T+L +AA+ G   +   LL +GA   +  KD  +AL +A   G +E+V+ LL +   P+     G T L  +A  G  +I  LLL  GS+++    +G TPL  A       +  LLL   A  D     G + + VAA                            L + +    L+I   LL Y    N +  +G++ L +AA EGS  L  +LL   A++++ ++   +PL  AA      V  +LL  GA I    KSG++ L ++   GN ++ + LL        +   G TPLH A+ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E  N  +   +   KI V    PE +   L    D  ++D+  +T
Sbjct:  448 NIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTLRGVTDE--NLTATPITEKHKINV----PETMNEFLDMSDDEAVKDHEAQT 860          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 229.95 bits (585), Expect = 1.066e-60
Identity = 165/580 (28.45%), Postives = 280/580 (48.28%), Query Frame = 2
Query: 1601 TSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            T  +S   +  +  + S   + R  +   +   +  G+DIN  N+NG  +L +A++ G   +   LL  GAN     K G +AL +A   G  EVV+ L++   + +    +G T L  AA     D+V  LL + S+ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D + R   +PL  A+  GN  +V+LLL  G+ I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+  A  +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +  + L  H  + N  N+      G TA+H+A+  G  ++++ L+  GA++D+K  + +T LH+ +  G  +I Q L+  G   +   + G T L ++++EGH EI  LL+  G+++        TP  VA K   +++ NLL +  A  D
Sbjct:   29 TRKHSRDQKKKSDSNASYLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVR-----GETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPD 593          

HSP 2 Score: 209.149 bits (531), Expect = 4.002e-54
Identity = 158/550 (28.73%), Postives = 257/550 (46.73%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            ++ + N +  L+  G  I    ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL      D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA +      G + I VAA   + N     I+K L  +  + N  +  G   L +AA  G   + + LL+N A +DI    D++ L  A+  G  E+V+ LL  GA  +    SG++ L +SA +G+ +I + LL +G         G TPLH+A+  GQ E  NLLL+  A P+     G  PL ++      ++A  L+D    S +  + +G T L IA      E    LL  GA+       G +PLH++  +   D   +LL         N+N  + +G T +H+A+      + + L++ GA ID +   G T LH+    G  ++   L++     +     G T L  A+Q+GH  I N+L+ +GA+   +     T   +A +  +I + + L
Sbjct:  278 KRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHEN-----IVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQG-ASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQA-----NVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTL 816          

HSP 3 Score: 190.274 bits (482), Expect = 2.942e-48
Identity = 157/591 (26.57%), Postives = 272/591 (46.02%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALC-------------LNSNSKYLD---------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNAD---IDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID--VWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +L  + R++   + + L+ N  + +  +++G T L +AA  G  ++  LLL+ GA    + ++  + L +A   G   +VKLLL +    +    DG T L   A  G   +V +LL  G+ I  +   G +PL  AT       V+LLL + A +D   ND  +A+ VAA C              N N+K L+               +++LLL++  ++  +   G+  + +AA+ G + + K L  + A     ++   + L  AA +G  +VVR LL  GA +    K   + L I++  G  +I+ +LL +G         G TPLHL++ EG  E   LLL+ G+  +     G  PL ++ + G +++A  L+     P++       G T L +A           LL +GA       NG TPLHI+  ++Q +    LL++         N +   G + +H+A+ +G  +++  L+   A +++ +  G T LHL A      + ++L++ G  ID     G T L +A   G++++ N L+   A          TP   A +  H  I N+L ++GA
Sbjct:  206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAA---GKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGA-----ECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGA 788          

HSP 4 Score: 189.119 bits (479), Expect = 5.694e-48
Identity = 168/633 (26.54%), Postives = 290/633 (45.81%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAV-LNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            IN+ N N LNA     K+        +L + +   N   AT   + +L  +        + +L+ NG ++N  ++NG T L +AAQ     +   LL + ++Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ I+ +  DG + +   A      S +  ++++LL     I      G++ L +A  +G  L C ++LL++NA +D V    L A   AA  G+++V ++++   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V  L   GA PN  +  G   L ++ + G I +   L+    + V+ K+ D  T+L IA  L +L E V+ LL +GA       +G TPLH+S  +   +   +LL+       ++++     G T +H+A+  G  E+   L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  LEI   L+ +GA    +     +P  +A +   + + +LL
Sbjct:   68 INICNQNGLNALHLASKEGHVEVVAELLKLGA---NVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGA-----RSGHEQVVEMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAK-VDIKAKDDQTALHIASRLGKL-EIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQG-----SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLL 684          

HSP 5 Score: 161.77 bits (408), Expect = 1.645e-39
Identity = 137/496 (27.62%), Postives = 226/496 (45.56%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            A   G +E V   L   +D +    +G  AL  A+  G  ++V  LL  G+N++    +G T L  A+      +V+ L+ NGA ++ Q  +G + +++AA        +LD+++ LL  N + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D M R++   TPLH+AS  G    V LLL  G+K                                  +  K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LLQH+       ++ + +D  TA+H+A+  G  ++ K ++D  A  + K   G T LH+   +   ++ +LL+  G ++      G T + +A+  GH  I   L + GA+    +    T   +A +   I +   L ++GA VD K
Sbjct:   49 AARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAA-----QENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDI--TPLHVASKRGNGNMVKLLLDRGSK----------------------------------IEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAP-----VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIK 496          

HSP 6 Score: 157.147 bits (396), Expect = 4.965e-38
Identity = 132/468 (28.21%), Postives = 214/468 (45.73%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDV 3019
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +VK L      P+ T   G TAL  AA  G+ D+V  LL +G+ ++ +  + +T L  A+      +V+ LL  GA  +     G +      L L++   + +I  LLL    +++    +G   L +AA  G   +  +LL+  A  D   +S   PL  AA   N  V  LLL  GA+ H   K+G++ L I+A +   +I + LL  G +       G +PLHLA+ EG  + V+LLL   A  N  + +G  PL L+ Q+    + E L++   E ++ ++  G T L +A      +   FLL   A    +  NG TPLH +  Q       +LLQ+  + N   +N     G TA+ IA   G   ++ +L  V
Sbjct:  363 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYT-----PLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAE-IDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLN-----GNTALSIARRLGYISVVDTLRGV 819          

HSP 7 Score: 150.599 bits (379), Expect = 4.441e-36
Identity = 121/419 (28.88%), Postives = 189/419 (45.11%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRT 2845
            N + QL  +G   N  N  G T+L +AA+ G   +   LL +GA   +  KD  +AL +A   G +E+V+ LL +   P+     G T L  +A  G  +I  LLL  GS+++    +G TPL  A       +  LLL   A  D     G + + VAA                            L + +    L+I   LL Y    N +  +G++ L +AA EGS  L  +LL   A++++ ++   +PL  AA      V  +LL  GA I    KSG++ L ++   GN ++ + LL        +   G TPLH A+ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E  N  +   +   KI V    PE +   L    D  ++D+  +T
Sbjct:  448 NIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTLRGVTDE--NLTATPITEKHKINV----PETMNEFLDMSDDEAVKDHEAQT 860          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Match: BX469921.1 (ankyrin 3a [Source:NCBI gene;Acc:103909035])

HSP 1 Score: 229.95 bits (585), Expect = 1.079e-60
Identity = 163/564 (28.90%), Postives = 274/564 (48.58%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  +   +   +  G+DIN  N+NG  +L +A++ G   +   LL  GAN     K G +AL +A   G  EVV+ L++   + +    +G T L  AA     D+V  LL + S+ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D + R   +PL  A+  GN  +V+LLL  G+ I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+  A  +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +  + L  H  + N  N+      G TA+H+A+  G  ++++ L+  GA++D+K  + +T LH+ +  G  +I Q L+  G   +   + G T L ++++EGH EI  LL+  G+++        TP  VA K   +++ NLL +  A  D
Sbjct:   45 SYLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVR-----GETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPD 593          

HSP 2 Score: 196.052 bits (497), Expect = 4.560e-50
Identity = 154/555 (27.75%), Postives = 255/555 (45.95%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATA-----LSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            ++ + N +  L+  G  I    ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL      D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA +      G + I VAA   + N     I+K L  +  + N  +  G   L +AA  G   + + LL+N A +DI    D++ L  A+  G  E+V+ LL  GA  +    SG++ L +SA +G+ +I + LL +G         G TPLH+A+  GQ E  NLLL+  A P+     G  PL ++      ++A  L+D    S +  + +G T L IA      E    LL  GA+       G +PLH++  +   D   +LL         N+N  + +G T +H+A+      + + L++ GA ID +   G T LH+    G  ++   L++     +              +  ++Q+GH  I N+L+ +GA+   +     T   +A +  +I + + L
Sbjct:  278 KRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHEN-----IVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQG-ASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQA-----NVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKVIVNGYFDMLIPFSAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTL 821          

HSP 3 Score: 189.889 bits (481), Expect = 3.950e-48
Identity = 168/633 (26.54%), Postives = 290/633 (45.81%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAV-LNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            IN+ N N LNA     K+        +L + +   N   AT   + +L  +        + +L+ NG ++N  ++NG T L +AAQ     +   LL + ++Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ I+ +  DG + +   A      S +  ++++LL     I      G++ L +A  +G  L C ++LL++NA +D V    L A   AA  G+++V ++++   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V  L   GA PN  +  G   L ++ + G I +   L+    + V+ K+ D  T+L IA  L +L E V+ LL +GA       +G TPLH+S  +   +   +LL+       ++++     G T +H+A+  G  E+   L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  LEI   L+ +GA    +     +P  +A +   + + +LL
Sbjct:   68 INICNQNGLNALHLASKEGHVEVVAELLKLGA---NVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGA-----RSGHEQVVEMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAK-VDIKAKDDQTALHIASRLGKL-EIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQG-----SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLL 684          

HSP 4 Score: 187.578 bits (475), Expect = 1.710e-47
Identity = 162/562 (28.83%), Postives = 267/562 (47.51%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLL----QHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            +L  + R++   + + L+ N  + +  +++G T L +AA  G  ++  LLL+ GA    + ++  + L +A   G   +VKLLL +    +    DG T L   A  G   +V +LL  G+ I  +   G +PL  AT       V+LLL + A +D   ND  +A+ VAA C      +  + K+++    N N     G   L IA  +    + ++LL++ A +  V  S   P+  AAF G+  +V+ L   GA+ +  +  G + L ++A  G   ++  LL  G  +D   +D+   T LH+AS  G+ E V  LL+ GA PN     G  PL LS +EG+ +IA  L++    S++  +  G T L +A      E    LL + A       +G TPLH++   + YD  +V L    Q +  H+         +G T +HIA+ K   EI  +L++ GA  +    +G + LHL A +G   +  LL+    N++     G T L +A+Q+    +  +L+N GA +        TP  VA    ++K+ N L
Sbjct:  206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHC-----GHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDD--QTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQG-SSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVA---AHYDNQRVALLLLDQGASPHSPAK------NGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFL 750          

HSP 5 Score: 149.828 bits (377), Expect = 6.926e-36
Identity = 137/531 (25.80%), Postives = 219/531 (41.24%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +VK L      P+ T   G TAL  AA  G+ D+V  L                                 L NGA +D +  D ++A+ +A     S    L+I++ LL+     N     G   L ++A EG + +  +LLE  + +    +   +PL  AA  G  EV  LLL   AA     KSG + L ++A   N ++   LL +G         G TPLH+A+ + Q E    LL+ GA+ N +   G  PL L+ QEG + +   L+     +VN  + +G T L +A  +      E LL+ GA+   Q  +G TPLH++           LL++    N                           K+ + V    D+        +   A QG   I  +L+ +G + +E    G TALSIA + G++ + + L
Sbjct:  363 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYL---------------------------------LQNGAKVDIKAKDDQTALHIA-----SRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLL-TKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPN--------------------------AKTKVIVNGYFDML-------IPFSAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTL 821          

HSP 6 Score: 148.673 bits (374), Expect = 1.988e-35
Identity = 134/498 (26.91%), Postives = 212/498 (42.57%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A   G +E V   L   +D +    +G  AL  A+  G  ++V  LL  G+N++    +G T L  A+      +V+ L+ NGA ++ Q  +G + +++AA        +LD+++ LL  N + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D M R++   TPLH+AS  G    V LLL  G+K                                  +  K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LLQH+                                      A +D   N+  T LH+ A  G Y++ ++++D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H  I   L  HGA  +  NV
Sbjct:   49 AARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAA-----QENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDI--TPLHVASKRGNGNMVKLLLDRGSK----------------------------------IEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHN--------------------------------------APVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNV 465          

HSP 7 Score: 130.568 bits (327), Expect = 5.640e-30
Identity = 115/424 (27.12%), Postives = 187/424 (44.10%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHR-----DNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRT 2845
            N + QL  +G   N  N  G T+L +AA+ G   +   LL +GA   +  KD  +AL +A   G +E+V+ LL +   P+     G T L  +A  G  +I  LLL  GS+++    +G TPL  A       +  LLL   A  D     G + + VAA                            L + +    L+I   LL Y    N +  +G++ L +AA EGS  L  +LL   A++++ ++   +PL  AA      V  +LL  GA I    KSG++ L ++   GN ++ + LL        +     + +    +  ++ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E  N  +   +   KI V    PE +   L    D  ++D+  +T
Sbjct:  448 NIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKVIVNGYFDMLIPFSAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTLRGVTDE--NLTATPITEKHKINV----PETMNEFLDMSDDEAVKDHEAQT 865          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Match: gart (phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase [Source:Xenbase;Acc:XB-GENE-993017])

HSP 1 Score: 700.279 bits (1806), Expect = 0.000e+0
Identity = 394/1036 (38.03%), Postives = 598/1036 (57.72%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDE--NLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE--VKGILPCE--------KFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            LA HFC A D D++ +  FI  +   + +       +   +N   + +L         P  AFK  +LLPLL+   P +++++++DS+DE  N++    GE           H+    S  I+DLL+ HH FFP WL L+C+ RK++  + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ K L+  E++ A+   +++  +   +  L  +   LV+   N     H S  EWLLDVK CT KY C+  +GH  LA+     + +L P++++ FA HLI S    +      A+W++ +   ++  +  ++P E        K G+  D   D    I+ +++   +  +       ++      N   +   ++ A   YS +L         + ++ L+  G D+   + NG T+L +AA+ G   + N L+  GAN    D DGW+ALR A W G+ EVV  LL      D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL +GA I+H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NEIDN GRIPL+L+ QEG+    + L++    +V+ K YDG ++L++A L    + VE LLS GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D DGRTA+H++ W+G  E+++SLI   A ++  DNE R+ L   AWQG  ++ QLLI+ G  +D TC+QGATAL IA+QEGH ++   L+  GA+  H D   RT  +VA K  H +I  LLEK+GA +
Sbjct:   96 LAYHFCRAQDSDTMCVGSFIRSLVDQICQGGLM-PGFEDKLNDPSVQSLLQPGECERNPSEAFKRCVLLPLLAIKAPAQSLFMVVDSVDEGCNIVE---GE-----------HRPSGLSGTIADLLATHHEFFPPWLLLICSARKQSRTVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACKPLTTIELFNAVWTKNMSLTMEDFQRKLDVLSKVLVEGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCRAKELTPIEVQEFALHLIHSN--LQLTNSELALWMILNGTCVKDSLCTLIPKEQEVLQLLVKAGAHVDSEDDRTCCIVRQALEREDSIRTLLDNGASV------NQCDSNGRTLLANTAYSGNL---------DVVNLLVSRGSDLEIEDANGQTALTLAARQGHVKVVNCLIGCGANINHCDHDGWTALRSAAWGGHTEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLGHGAEINHEDVDGRTALSVAALCVPASKGHASVVSLLIEQGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHAAVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPLILAAQEGHYDCVQILLEN-KSAVDQKGYDGRSALRVAALEGHRDIVELLLSNGADLNAKDADGRPTLYILALENQLSMAEYFLE-----NGANVEASDTDGRTALHVSCWQGHLEMVQSLISYKADVNASDNEKRSSLQSAAWQGHVKVVQLLIEHGTLVDNTCNQGATALCIAAQEGHTDVVQFLLENGADPNHADQFGRTAMRVAAKNGHSEIIKLLEKYGASI 1092          

HSP 2 Score: 249.98 bits (637), Expect = 6.001e-67
Identity = 171/520 (32.88%), Postives = 277/520 (53.27%), Query Frame = 2
Query: 1796 LLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            LL +GA+    D +G + L    ++G ++VV LL+S+  D +    +G+TAL  AA  G   +V  L+  G+NIN  D +G T L +A +     +V  LL  GA +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL + A+I   D+  R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A         E L+ +GA     DN+GR PL ++  +  YDC Q+LL+     N + ++    DGR+A+ +A+ +G  +I++ L+  GA ++ KD +GR  L++ A +    + +  ++ G N++ + + G TAL ++  +GHLE+   LI++ A+V   D+  R+  + A    H+K+  LL +HG LVD
Sbjct:  519 LLDNGASVNQCDSNGRTLLANTAYSGNLDVVNLLVSRGSDLEIEDANGQTALTLAARQGHVKVVNCLIGCGANINHCDHDGWTALRSAAWGGHTEVVSALLYAGAKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLGHGAEINHEDVDGRTALSVAALCVPASKGHASVVSLLIEQGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHAAVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPLILAAQEGHYDCVQILLE-----NKSAVDQKGYDGRSALRVAALEGHRDIVELLLSNGADLNAKDADGRPTLYILALENQLSMAEYFLENGANVEASDTDGRTALHVSCWQGHLEMVQSLISYKADVNASDNEKRSSLQSAAWQGHVKVVQLLIEHGTLVD 1027          

HSP 3 Score: 168.703 bits (426), Expect = 1.282e-41
Identity = 128/427 (29.98%), Postives = 217/427 (50.82%), Query Frame = 2
Query: 2081 MVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVL-----NRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +++LL+  GA++D +D D    I   AL      +  D I+ LL    ++N  D+ G  +L   AY G+  +  +L+   +D++I D   ++ L  AA  G+ +VV  L+  GA I+  D  G++ L  +A  G+++++S LL  G      D    T L  A+  G  + V  LL+ GA+ N+ DN GR  L+ +   G+ +I E L+    E +N +  DG T+L +A L           V  L+ +GA+    D +G TPL ++  +   D   +LL+        +++  D++GRT +  A+  G   ++ +L+  GA +D  D+EGRT L + + QG  ++ + L+D G++ +     G T L +A+ EGH  IC  LI  GA    ID+  R P  +A +  H     +L ++ + VD+K
Sbjct:  484 VLQLLVKAGAHVDSED-DRTCCIVRQAL------EREDSIRTLLDNGASVNQCDSNGRTLLANTAYSGNLDVVNLLVSRGSDLEIEDANGQTALTLAARQGHVKVVNCLIGCGANINHCDHDGWTALRSAAWGGHTEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLGHGAE-INHEDVDGRTALSVAALCVPASKGHASVVSLLIEQGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGA-----DVDHTDNNGRTPLLAAASMGHAAVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPLILAAQEGHYDCVQILLENKSAVDQK 897          

HSP 4 Score: 148.288 bits (373), Expect = 2.664e-35
Identity = 101/303 (33.33%), Postives = 160/303 (52.81%), Query Frame = 2
Query: 1670 QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            Q +   +  L+  GLD N  ++ G T L +AA  G   IC  L+  GA    ID DG   L LA   G+ + V++LL  K   D  G DGR+ALR AA  G  DIV LLLS+G+++N +D++GR  L     +    M +  L NGA ++  D DGR+A+ V+  C      +L++++ L+ Y  ++N  DNE  + L  AA++G   + ++L+E+   +D       + L  AA  G+ +VV+ LL  GA  +  D+ G + + ++A  G+S+I+  L   G         GC P  + ++E
Sbjct:  812 QGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPLILAAQEGHYDCVQILLENKSAVDQKGYDGRSALRVAALEGHRDIVELLLSNGADLNAKDADGRPTLYILALENQLSMAEYFLENGANVEASDTDGRTALHVS--CWQG---HLEMVQSLISYKADVNASDNEKRSSLQSAAWQGHVKVVQLLIEHGTLVDNTCNQGATALCIAAQEGHTDVVQFLLENGADPNHADQFGRTAMRVAAKNGHSEIIKLLEKYGAS----IPSGCNPSPVHTME 1105          

HSP 5 Score: 76.6406 bits (187), Expect = 2.290e-13
Identity = 56/187 (29.95%), Postives = 94/187 (50.27%), Query Frame = 2
Query: 2780 EFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            E ++ L+  GA    +D+  RT   +     + D  + LL      N  ++N  D +GRT +   ++ G+ +++  L+  G+ ++++D  G+T L L A QG  ++   LI  G NI+     G TAL  A+  GH E+ + L+  GA V   D+  RT  + A  G H  I   L +HGA V++ +
Sbjct:  483 EVLQLLVKAGAHVDSEDD--RTCCIVRQALEREDSIRTLLD-----NGASVNQCDSNGRTLLANTAYSGNLDVVNLLVSRGSDLEIEDANGQTALTLAARQGHVKVVNCLIGCGANINHCDHDGWTALRSAAWGGHTEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKAD 662          

HSP 6 Score: 74.3294 bits (181), Expect = 1.378e-12
Identity = 55/186 (29.57%), Postives = 89/186 (47.85%), Query Frame = 2
Query: 2786 VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +  LL  GA     D+NGRT L  +      D   +L+        +++   D +G+TA+ +A+ +G  +++  LI  GA I+  D++G T L   AW G  ++   L+  G  +D   +   TAL  A+  GH +I   L+  GA V   D+  RT    A    H +I   L  HGA ++ ++V
Sbjct:  516 IRTLLDNGASVNQCDSNGRTLLANTAYSGNLDVVNLLVSRG-----SDLEIEDANGQTALTLAARQGHVKVVNCLIGCGANINHCDHDGWTALRSAAWGGHTEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLGHGAEINHEDV 696          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097])

HSP 1 Score: 226.098 bits (575), Expect = 2.965e-59
Identity = 163/546 (29.85%), Postives = 270/546 (49.45%), Query Frame = 2
Query: 1706 NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            NG+DIN  N+NG  +L +A++ G   I + L+  GA+     K G +AL +A   G  EVV++L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  G+ I    + G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA   NRL + +E LL  GA       +G TP+H++      +    L  H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G   D   + G T L ++S+EGH ++ ++L+  GA+   +     TP  VA K   I++  LL +  A  D
Sbjct:   46 NGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRL-KVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVR-----GETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPD 575          

HSP 2 Score: 206.068 bits (523), Expect = 5.465e-53
Identity = 157/551 (28.49%), Postives = 255/551 (46.28%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L  +  + N  +  G   L +AA  G   + + LL+N A ++     D++PL  +A  G  ++V+ LL  GA       SG++ L +S+ +G+  + S LL  G         G TPLH+A+  G+ E V LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G  P+H++  +   D   +LL  S      N+N  +  G T +H+A+ +    + + L + GA ID       T LH+ +  G  ++   L+  G  ++     G T L  A+Q+GH  I N+L+  GA+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKG-ASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSA-----NVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLK 799          

HSP 3 Score: 203.371 bits (516), Expect = 3.991e-52
Identity = 167/637 (26.22%), Postives = 285/637 (44.74%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID--VWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQ----------------HSLTHNINNINCL------------DDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L   G      +  G T LH+A+  GQ E V  LL++GA+        + PL +S + G   I ++L+    +P++     Y   T L ++      +    LL  GA   I    G TPLH++    + +  ++LLQ                H   H  N    L              +G T +HIA+ K   +I  +L++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L N GA +        TP  VA    +IK+   L +HG+ V+ K
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGY---TPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAK 742          

HSP 4 Score: 178.718 bits (452), Expect = 1.300e-44
Identity = 138/470 (29.36%), Postives = 215/470 (45.74%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEF-----VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L      P+ T   G TAL  AA  G++++V  LL +G+ +  +  + +TPL  +       +V+ LL  GAY D     G +      L L+S   + D+  +LL +  +  ++  +G   L +AA  G   + K+LL+ NA  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G  P+HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L +           DG+T +    L+    +     V FLL  G+    +  NG TPLH +  Q       VLLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYT-----PLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLAN------QGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 145.976 bits (367), Expect = 1.799e-34
Identity = 131/498 (26.31%), Postives = 213/498 (42.77%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A  +G +E     L   +D +    +G  AL  A+  G  +IV  L+  G++++    +G T L  A+      +V++L+ NGA ++ Q  +G + +++AA        +L+++K LL    + +L   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D   R++   TPLH+AS  G    V LLL  G+K                                  ++ K+ DG T L     +   + V  LL RGA    +  NG +PLH++      +C Q+LLQH++                                       +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  L++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   31 AARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDI--TPLHVASKRGNANMVKLLLDRGSK----------------------------------IDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNV 447          

HSP 6 Score: 135.576 bits (340), Expect = 2.984e-31
Identity = 116/421 (27.55%), Postives = 202/421 (47.98%), Query Frame = 2
Query: 2081 MVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIP-LMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQIC-QLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            M +      A   H+   G+ +   A+    + S  L+     L+   +IN+ +  G+N L +A+ EG   +   L++  AD+D   +   + L  A+ +G  EVVR+L+  GA ++   ++GF+ L ++A + + +++  LL  G  +      G TPL +A  +G  + V+LLL+     N+     R+P L ++ ++   K A  L+     + + +S  G T L IA           LL+RGA       N  TPLH++  +   +  ++LL        + I+    DG T +H  +  G  +++  L+D GA I  K   G + LH+ A QG +  C QLL+   + +D+  +   TAL +A+  GH ++  +L++  AN         TP  +A K N +K+  LL KHGA +
Sbjct:    1 MSEETKEKAAKPAHRKRKGKKSDTNASYLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLE-----NDTKGKVRLPALHIAARKDDTKAAALLLQ-NDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRG-----SKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHM-ATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASI 409          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097])

HSP 1 Score: 226.098 bits (575), Expect = 3.068e-59
Identity = 163/546 (29.85%), Postives = 270/546 (49.45%), Query Frame = 2
Query: 1706 NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            NG+DIN  N+NG  +L +A++ G   I + L+  GA+     K G +AL +A   G  EVV++L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  G+ I    + G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA   NRL + +E LL  GA       +G TP+H++      +    L  H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G   D   + G T L ++S+EGH ++ ++L+  GA+   +     TP  VA K   I++  LL +  A  D
Sbjct:   46 NGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRL-KVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVR-----GETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPD 575          

HSP 2 Score: 205.682 bits (522), Expect = 7.620e-53
Identity = 157/551 (28.49%), Postives = 255/551 (46.28%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L  +  + N  +  G   L +AA  G   + + LL+N A ++     D++PL  +A  G  ++V+ LL  GA       SG++ L +S+ +G+  + S LL  G         G TPLH+A+  G+ E V LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G  P+H++  +   D   +LL  S      N+N  +  G T +H+A+ +    + + L + GA ID       T LH+ +  G  ++   L+  G  ++     G T L  A+Q+GH  I N+L+  GA+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKG-ASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSA-----NVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLK 799          

HSP 3 Score: 202.986 bits (515), Expect = 5.236e-52
Identity = 167/637 (26.22%), Postives = 285/637 (44.74%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID--VWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQ----------------HSLTHNINNINCL------------DDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L   G      +  G T LH+A+  GQ E V  LL++GA+        + PL +S + G   I ++L+    +P++     Y   T L ++      +    LL  GA   I    G TPLH++    + +  ++LLQ                H   H  N    L              +G T +HIA+ K   +I  +L++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L N GA +        TP  VA    +IK+   L +HG+ V+ K
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGY---TPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAK 742          

HSP 4 Score: 178.718 bits (452), Expect = 1.639e-44
Identity = 138/470 (29.36%), Postives = 215/470 (45.74%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEF-----VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L      P+ T   G TAL  AA  G++++V  LL +G+ +  +  + +TPL  +       +V+ LL  GAY D     G +      L L+S   + D+  +LL +  +  ++  +G   L +AA  G   + K+LL+ NA  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G  P+HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L +           DG+T +    L+    +     V FLL  G+    +  NG TPLH +  Q       VLLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYT-----PLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLAN------QGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 145.591 bits (366), Expect = 2.313e-34
Identity = 131/498 (26.31%), Postives = 213/498 (42.77%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A  +G +E     L   +D +    +G  AL  A+  G  +IV  L+  G++++    +G T L  A+      +V++L+ NGA ++ Q  +G + +++AA        +L+++K LL    + +L   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D   R++   TPLH+AS  G    V LLL  G+K                                  ++ K+ DG T L     +   + V  LL RGA    +  NG +PLH++      +C Q+LLQH++                                       +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  L++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   31 AARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDI--TPLHVASKRGNANMVKLLLDRGSK----------------------------------IDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNV 447          

HSP 6 Score: 145.206 bits (365), Expect = 3.390e-34
Identity = 107/377 (28.38%), Postives = 173/377 (45.89%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESV 2719
            N +SQL  +G   N  N  G T+L +AA+ G + +   LL +GA      KD  + L ++   G  ++V+ LL Q   PD     G T L  ++  G  D+  +LL  G++      +G TPL  A       +VKLLL   A  D     G + + VAA                            L + +    +DI   LL Y  + N I  +G+  + +AA EG   +  +LL  +A++++ ++S   PL  AA      V  +L   GA I    K  ++ L +++  GN ++++ LL  G     +   G TPLH A+ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E++
Sbjct:  430 NIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLKVVTEETL 806          

HSP 7 Score: 135.191 bits (339), Expect = 3.609e-31
Identity = 116/421 (27.55%), Postives = 202/421 (47.98%), Query Frame = 2
Query: 2081 MVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIP-LMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQIC-QLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            M +      A   H+   G+ +   A+    + S  L+     L+   +IN+ +  G+N L +A+ EG   +   L++  AD+D   +   + L  A+ +G  EVVR+L+  GA ++   ++GF+ L ++A + + +++  LL  G  +      G TPL +A  +G  + V+LLL+     N+     R+P L ++ ++   K A  L+     + + +S  G T L IA           LL+RGA       N  TPLH++  +   +  ++LL        + I+    DG T +H  +  G  +++  L+D GA I  K   G + LH+ A QG +  C QLL+   + +D+  +   TAL +A+  GH ++  +L++  AN         TP  +A K N +K+  LL KHGA +
Sbjct:    1 MSEETKEKAAKPAHRKRKGKKSDTNASYLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLE-----NDTKGKVRLPALHIAARKDDTKAAALLLQ-NDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRG-----SKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHM-ATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASI 409          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097])

HSP 1 Score: 225.713 bits (574), Expect = 3.908e-59
Identity = 170/569 (29.88%), Postives = 283/569 (49.74%), Query Frame = 2
Query: 1715 DINCVNENGTT--SLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSK------------YLDIIK---LLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            D +C+++   T  S + AA++G        L +G +  + +++G +AL LA   G++E+V  L+ +  D D +   G TAL  A+  G+T++V +L+++G+N+N Q   G TPL  A  +    +VK LL+NGA       DG + + VA         +L L +++K              D  K   LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  G+ I    + G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA   NRL + +E LL  GA       +G TP+H++      +    L  H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G   D   + G T L ++S+EGH ++ ++L+  GA+   +     TP  VA K   I++  LL +  A  D
Sbjct:    5 DGDCLSDQSDTNASYLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRL-KVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVR-----GETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPD 566          

HSP 2 Score: 205.682 bits (522), Expect = 7.548e-53
Identity = 157/551 (28.49%), Postives = 255/551 (46.28%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L  +  + N  +  G   L +AA  G   + + LL+N A ++     D++PL  +A  G  ++V+ LL  GA       SG++ L +S+ +G+  + S LL  G         G TPLH+A+  G+ E V LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G  P+H++  +   D   +LL  S      N+N  +  G T +H+A+ +    + + L + GA ID       T LH+ +  G  ++   L+  G  ++     G T L  A+Q+GH  I N+L+  GA+   +     T   +A +  +I + + L+
Sbjct:  251 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKG-ASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSA-----NVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLK 790          

HSP 3 Score: 199.519 bits (506), Expect = 5.237e-51
Identity = 162/581 (27.88%), Postives = 269/581 (46.30%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ ID +  DG + +   A      S +  ++ +LL     I      G++ L +A  +G  L C ++LL++N  +D V    L A   AA  G+++V ++LL   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V+ L   GA PN  +  G   L ++ + G  ++   L+    + V  K+ D  T L I+      + V+ LL +GA       +G TPLH+S  +   D   VLL+H  +  I     +   G T +H+A+  G  E++K L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  ++I   L+ +GA+   I      P  +A +  H+ + +LL    A V+  N
Sbjct:  100 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGA-----RSGHEQVVGMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQ-VEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGI-----VTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSN 668          

HSP 4 Score: 198.749 bits (504), Expect = 1.059e-50
Identity = 164/566 (28.98%), Postives = 266/566 (47.00%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +L  + R++     + L+ N  + +  +++G T L +AA  G  ++  LLL+ GA      ++  + L +A   G   +VKLLL +    D    DG T L   A  G   +V +LL  G+ I  +   G +PL  AT       V+LLL +   +D   ND  +A+ VAA C      +  + K+LL    N N     G   L IA  +    + ++LL++ A I  V  S   P+  AAF G+  +V  L   GA+ +  +  G + L ++A  G S+++  LL  G     +     TPLH+++  G+ + V  LLK GA P+     G  PL LS +EG+  +A  L++    S    +  G T L +A      E V+ LL + A       +G TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   +I  +L++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L N GA +        TP  VA    +IK+   L +HG+ V+ K
Sbjct:  179 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHC-----GHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHG-ASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVA---AHYDNQKVALL--LLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAK 733          

HSP 5 Score: 178.718 bits (452), Expect = 1.428e-44
Identity = 138/470 (29.36%), Postives = 215/470 (45.74%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEF-----VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L      P+ T   G TAL  AA  G++++V  LL +G+ +  +  + +TPL  +       +V+ LL  GAY D     G +      L L+S   + D+  +LL +  +  ++  +G   L +AA  G   + K+LL+ NA  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G  P+HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L +           DG+T +    L+    +     V FLL  G+    +  NG TPLH +  Q       VLLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  336 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYT-----PLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLAN------QGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVN-----GNTALAIARRLGYISVVDTL 789          

HSP 6 Score: 145.976 bits (367), Expect = 1.732e-34
Identity = 131/498 (26.31%), Postives = 213/498 (42.77%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A  +G +E     L   +D +    +G  AL  A+  G  +IV  L+  G++++    +G T L  A+      +V++L+ NGA ++ Q  +G + +++AA        +L+++K LL    + +L   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D   R++   TPLH+AS  G    V LLL  G+K                                  ++ K+ DG T L     +   + V  LL RGA    +  NG +PLH++      +C Q+LLQH++                                       +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  L++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   22 AARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDI--TPLHVASKRGNANMVKLLLDRGSK----------------------------------IDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNV 438          

HSP 7 Score: 144.821 bits (364), Expect = 3.720e-34
Identity = 107/377 (28.38%), Postives = 173/377 (45.89%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESV 2719
            N +SQL  +G   N  N  G T+L +AA+ G + +   LL +GA      KD  + L ++   G  ++V+ LL Q   PD     G T L  ++  G  D+  +LL  G++      +G TPL  A       +VKLLL   A  D     G + + VAA                            L + +    +DI   LL Y  + N I  +G+  + +AA EG   +  +LL  +A++++ ++S   PL  AA      V  +L   GA I    K  ++ L +++  GN ++++ LL  G     +   G TPLH A+ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E++
Sbjct:  421 NIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLKVVTEETL 797          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097])

HSP 1 Score: 225.713 bits (574), Expect = 4.115e-59
Identity = 170/569 (29.88%), Postives = 283/569 (49.74%), Query Frame = 2
Query: 1715 DINCVNENGTT--SLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSK------------YLDIIK---LLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            D +C+++   T  S + AA++G        L +G +  + +++G +AL LA   G++E+V  L+ +  D D +   G TAL  A+  G+T++V +L+++G+N+N Q   G TPL  A  +    +VK LL+NGA       DG + + VA         +L L +++K              D  K   LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  G+ I    + G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA   NRL + +E LL  GA       +G TP+H++      +    L  H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G   D   + G T L ++S+EGH ++ ++L+  GA+   +     TP  VA K   I++  LL +  A  D
Sbjct:    5 DGDCLSDQSDTNASYLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRL-KVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVR-----GETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPD 566          

HSP 2 Score: 205.682 bits (522), Expect = 6.524e-53
Identity = 157/551 (28.49%), Postives = 255/551 (46.28%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L  +  + N  +  G   L +AA  G   + + LL+N A ++     D++PL  +A  G  ++V+ LL  GA       SG++ L +S+ +G+  + S LL  G         G TPLH+A+  G+ E V LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G  P+H++  +   D   +LL  S      N+N  +  G T +H+A+ +    + + L + GA ID       T LH+ +  G  ++   L+  G  ++     G T L  A+Q+GH  I N+L+  GA+   +     T   +A +  +I + + L+
Sbjct:  251 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKG-ASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSA-----NVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLK 790          

HSP 3 Score: 199.519 bits (506), Expect = 5.065e-51
Identity = 162/581 (27.88%), Postives = 269/581 (46.30%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A+ + +  MVKLLL+ G+ ID +  DG + +   A      S +  ++ +LL     I      G++ L +A  +G  L C ++LL++N  +D V    L A   AA  G+++V ++LL   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V+ L   GA PN  +  G   L ++ + G  ++   L+    + V  K+ D  T L I+      + V+ LL +GA       +G TPLH+S  +   D   VLL+H  +  I     +   G T +H+A+  G  E++K L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  ++I   L+ +GA+   I      P  +A +  H+ + +LL    A V+  N
Sbjct:  100 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGA-----RSGHEQVVGMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQ-VEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGI-----VTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSN 668          

HSP 4 Score: 198.749 bits (504), Expect = 1.042e-50
Identity = 164/566 (28.98%), Postives = 266/566 (47.00%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +L  + R++     + L+ N  + +  +++G T L +AA  G  ++  LLL+ GA      ++  + L +A   G   +VKLLL +    D    DG T L   A  G   +V +LL  G+ I  +   G +PL  AT       V+LLL +   +D   ND  +A+ VAA C      +  + K+LL    N N     G   L IA  +    + ++LL++ A I  V  S   P+  AAF G+  +V  L   GA+ +  +  G + L ++A  G S+++  LL  G     +     TPLH+++  G+ + V  LLK GA P+     G  PL LS +EG+  +A  L++    S    +  G T L +A      E V+ LL + A       +G TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   +I  +L++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L N GA +        TP  VA    +IK+   L +HG+ V+ K
Sbjct:  179 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHC-----GHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHG-ASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVA---AHYDNQKVALL--LLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAK 733          

HSP 5 Score: 178.718 bits (452), Expect = 1.371e-44
Identity = 138/470 (29.36%), Postives = 215/470 (45.74%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEF-----VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L      P+ T   G TAL  AA  G++++V  LL +G+ +  +  + +TPL  +       +V+ LL  GAY D     G +      L L+S   + D+  +LL +  +  ++  +G   L +AA  G   + K+LL+ NA  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G  P+HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L +           DG+T +    L+    +     V FLL  G+    +  NG TPLH +  Q       VLLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  336 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYT-----PLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLAN------QGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVN-----GNTALAIARRLGYISVVDTL 789          

HSP 6 Score: 145.976 bits (367), Expect = 1.845e-34
Identity = 131/498 (26.31%), Postives = 213/498 (42.77%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A  +G +E     L   +D +    +G  AL  A+  G  +IV  L+  G++++    +G T L  A+      +V++L+ NGA ++ Q  +G + +++AA        +L+++K LL    + +L   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D   R++   TPLH+AS  G    V LLL  G+K                                  ++ K+ DG T L     +   + V  LL RGA    +  NG +PLH++      +C Q+LLQH++                                       +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  L++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   22 AARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDI--TPLHVASKRGNANMVKLLLDRGSK----------------------------------IDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNV 438          

HSP 7 Score: 144.821 bits (364), Expect = 3.549e-34
Identity = 107/377 (28.38%), Postives = 173/377 (45.89%), Query Frame = 2
Query: 1682 NTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESV 2719
            N +SQL  +G   N  N  G T+L +AA+ G + +   LL +GA      KD  + L ++   G  ++V+ LL Q   PD     G T L  ++  G  D+  +LL  G++      +G TPL  A       +VKLLL   A  D     G + + VAA                            L + +    +DI   LL Y  + N I  +G+  + +AA EG   +  +LL  +A++++ ++S   PL  AA      V  +L   GA I    K  ++ L +++  GN ++++ LL  G     +   G TPLH A+ +G    +N+LL+ GA PNE+  +G   L ++ + GYI + + L  V  E++
Sbjct:  421 NIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGASPNEVTVNGNTALAIARRLGYISVVDTLKVVTEETL 797          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Match: Ankrd50 (ankyrin repeat domain 50 [Source:MGI Symbol;Acc:MGI:2139777])

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 392/1038 (37.76%), Postives = 602/1038 (58.00%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDE--NLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE--VKGILPCEKFGSLNDWSIDDIVKILFESINLVN--------INKLNAKKSFTIFS----TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            LA HFC A D D+L +  FI  +   + +S    + Y   +    + +L         P  AFK  +LLPLL   PP +++Y+L+DS+DE  N++    GE+ S              S  +++LL+ HH FFP WL L+C+ RK++  + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+  E+Y A+   +++  L   +  L  +   LV    +     H+S  EWLLDVK CT KY C+  +GH  LA+     +  L PL+ + FA HLI S    + +    A+W++++   ++  +  ++P E+          +++++L  +   VN        I +   ++  +I +       +N   +   ++ A   YS SL         + ++ L+  G D+   + +G T L +AA+ G T + N L+  GAN    D+DGW+ALR A W G+ EVV  LL   +  D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NEIDN GRIP +L+ QEG+    + L++    +++ + YDG  +L++A L    + VE L S GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++ QLLI+ G  +D TC+QGATAL IA+QEGH+++  +L+  GA+  H D   RT  +VA K  H +I  LLEK+GA
Sbjct:   71 LAFHFCKAQDSDTLCVGGFIRGLVAQICQSGLL-QGYEDKLRDPAVQSLLEPGECERNPAEAFKRCVLLPLLGMKPPPQSLYLLVDSVDEGCNVVE---GEQTSPSL-----------SGTVAELLAGHHEFFPPWLLLLCSARKQSRAVTKMFSGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAETLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTMTELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGSTKILFHHSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQARNLTPLEAQEFALHLINSN--LQLEPAELALWMIWNGTPVKDSLSTLIPKEQ----------EVLQLLIRAGAHVNSEDDHTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSL---------DVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDESHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN-KSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLE-----NGANVEASDAEGRTALHVSCWQGHVEMVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHVDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGA 1065          

HSP 2 Score: 248.44 bits (633), Expect = 1.102e-66
Identity = 177/553 (32.01%), Postives = 287/553 (51.90%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            LI  G  +N  +E+  TS +V            LL +GA+    D +G + L  A ++G ++VV LL+S+  D +     G T L  AA  G T +V  L+  G+NIN  D +G T L +A +     +V  LL  G  +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  + +   G T L +A         E L+ +GA     DN+GR P  ++  +  YDC Q+LL+     N +NI+    DGR A+ +A+ +G  +I++ L   GA ++ KD +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  +LI   A+V   D+  R+  + A    H+K+  LL +HGA+VD
Sbjct:  463 LIRAGAHVN--SEDDHTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-SHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLE-----NKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHVEMVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVD 1002          

HSP 3 Score: 190.66 bits (483), Expect = 1.491e-48
Identity = 142/463 (30.67%), Postives = 228/463 (49.24%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC-----QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +++ LL+ +G+++N +D      +  A  + D +  + LL+NGA ++  D++GR+ +  AA      S  LD++ LL+    ++ + D  G   L +AA +G   +   L+   A+I+  D+   + L +AA+ G+ EVV  LL+ G  + C D    + L  +A  G+  I+  LL  G +    DN G T L  A+  G  E V  LL  GA+ N  D  GR  L ++       +G+  +   LID   E V+    DG T L +A      + V+ LL  GAD    DNNGRTPL  +           LL          ++ +D +GRT + IAS +G+ E++++L+D G     +D+ G T LH+ A++G   IC+ LI+ G   +E  + G     +ASQEGH +   +L+   +N+       R   +VA    H  I  LL  HGA V+ K+ 
Sbjct:  458 EVLQLLIRAGAHVNSEDDHTSCIVRQALEREDSI--RTLLDNGASVNQCDSNGRTLLANAAY-----SGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE-VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAA-----VDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDESHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDA 907          

HSP 4 Score: 147.517 bits (371), Expect = 3.324e-35
Identity = 97/303 (32.01%), Postives = 163/303 (53.80%), Query Frame = 2
Query: 1670 QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            Q +   +  L+  GLD +  ++ G T L +AA  G   IC  L+  GA    ID DG     LA   G+ + V++LL  K + D  G DGR ALR AA  G  DIV LL S G+++N +D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      +++++++L+  + ++N  DNE  + L  AA++G   + ++L+E+ A +D       + L  AA  G+ +VV++LL  GA  +  D+ G + + ++A  G+SQI+  L   G   ++    GC+P  + ++E
Sbjct:  787 QGNVEVVRTLLDRGLDESHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVS--CWQG---HVEMVRVLIACHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHVDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLN----GCSPSPVHTME 1080          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 230.335 bits (586), Expect = 9.422e-61
Identity = 166/579 (28.67%), Postives = 278/579 (48.01%), Query Frame = 2
Query: 1604 STYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S YS S + ++  + S   + R          I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    S N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L +A++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   24 SCYSRSSQKSDANA-SYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 586          

HSP 2 Score: 212.231 bits (539), Expect = 3.893e-55
Identity = 173/638 (27.12%), Postives = 289/638 (45.30%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIFVA-------ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD----------------------------DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNH---IKICNLLEKHGALVDRK 3337
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     RSA  ++        L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A  +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L +A      +   FLL  GA  +I    G TPLH++    + +   +LLQ S + +    + L                              +G T +HIA+ K   +I  SL++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L+N GA   H+D+  +  Y   + G H   IKI N L +H A V+ K
Sbjct:  120 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGA---HVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAK 753          

HSP 3 Score: 200.29 bits (508), Expect = 2.209e-51
Identity = 150/551 (27.22%), Postives = 258/551 (46.82%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   ++ N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L ++A +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G TAL  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  271 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 810          

HSP 4 Score: 134.42 bits (337), Expect = 3.838e-31
Identity = 96/348 (27.59%), Postives = 157/348 (45.11%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            S R    + + QL+  G   N    +G T L +AA+ G   +   LL  GA+  +  K G++ L +A   G +EV  LLL +   PD  G  G T L  AA      + LLLL  G++ +     G TPL  A  K    +   LL  GA     D +  +   +A++ L +   ++D++ LLL  N N+NL +  G+  L +AA E    + ++L+   A +D   +   +PL      GN ++V  LL   A ++   K+G++ L  +A QG++ I                                 +N+LL++ A PNE+  +G   L ++ + GYI + + L
Sbjct:  500 SARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGA-----DANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHI---------------------------------INVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 809          

HSP 5 Score: 132.88 bits (333), Expect = 1.371e-30
Identity = 105/398 (26.38%), Postives = 186/398 (46.73%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL    +   +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  S       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   + +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   71 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL----ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-RAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 458          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 230.335 bits (586), Expect = 1.032e-60
Identity = 160/547 (29.25%), Postives = 267/547 (48.81%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    S N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L +A++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   44 IKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 575          

HSP 2 Score: 219.55 bits (558), Expect = 2.464e-57
Identity = 166/618 (26.86%), Postives = 289/618 (46.76%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIF-------VAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     RSA         ++ L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A  +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L +A      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL++  ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 720          

HSP 3 Score: 201.06 bits (510), Expect = 1.158e-51
Identity = 150/551 (27.22%), Postives = 258/551 (46.82%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   ++ N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L ++A +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G TAL  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 799          

HSP 4 Score: 160.229 bits (404), Expect = 4.769e-39
Identity = 129/465 (27.74%), Postives = 211/465 (45.38%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+      N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G + + +AA        + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG T LH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAA-----REGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 133.65 bits (335), Expect = 7.892e-31
Identity = 105/398 (26.38%), Postives = 186/398 (46.73%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL    +   +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  S       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   + +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL----ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-RAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 447          

HSP 6 Score: 125.561 bits (314), Expect = 2.174e-28
Identity = 94/347 (27.09%), Postives = 162/347 (46.69%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLP-----------EFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            + AA +G+ E     +  G  ++  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++   L+D    S +  + DG T L +A+                    RLP           +    LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D    I      G + L +A+Q  HL    LL+     V  + +   T   VA    H K+  +L
Sbjct:   29 LRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNG-ASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVL 369          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 229.18 bits (583), Expect = 2.221e-60
Identity = 160/547 (29.25%), Postives = 267/547 (48.81%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    S N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L +A++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   44 IKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 575          

HSP 2 Score: 218.009 bits (554), Expect = 7.011e-57
Identity = 166/618 (26.86%), Postives = 289/618 (46.76%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIF-------VAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     RSA         ++ L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A  +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L +A      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL++  ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 720          

HSP 3 Score: 199.904 bits (507), Expect = 2.934e-51
Identity = 150/551 (27.22%), Postives = 258/551 (46.82%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   ++ N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L ++A +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G TAL  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 799          

HSP 4 Score: 159.458 bits (402), Expect = 9.236e-39
Identity = 129/465 (27.74%), Postives = 211/465 (45.38%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+      N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G + + +AA        + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG T LH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAA-----REGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 132.88 bits (333), Expect = 1.500e-30
Identity = 105/398 (26.38%), Postives = 186/398 (46.73%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL    +   +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  S       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   + +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL----ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLN-RAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 447          

HSP 6 Score: 125.176 bits (313), Expect = 3.251e-28
Identity = 94/347 (27.09%), Postives = 162/347 (46.69%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLP-----------EFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            + AA +G+ E     +  G  ++  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++   L+D    S +  + DG T L +A+                    RLP           +    LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D    I      G + L +A+Q  HL    LL+     V  + +   T   VA    H K+  +L
Sbjct:   29 LRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNG-ASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVL 369          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026])

HSP 1 Score: 229.18 bits (583), Expect = 2.351e-60
Identity = 160/547 (29.25%), Postives = 267/547 (48.81%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    S N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L +A++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   44 IKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 575          

HSP 2 Score: 218.009 bits (554), Expect = 6.892e-57
Identity = 166/618 (26.86%), Postives = 289/618 (46.76%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIF-------VAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     RSA         ++ L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A  +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L +A      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL++  ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 720          

HSP 3 Score: 199.904 bits (507), Expect = 3.639e-51
Identity = 150/551 (27.22%), Postives = 258/551 (46.82%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   ++ N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L ++A +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G TAL  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 799          

HSP 4 Score: 159.458 bits (402), Expect = 1.056e-38
Identity = 129/465 (27.74%), Postives = 211/465 (45.38%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+      N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G + + +AA        + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG T LH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAA-----REGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 132.88 bits (333), Expect = 1.492e-30
Identity = 105/398 (26.38%), Postives = 185/398 (46.48%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  S       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   + +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDT----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRA-AAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 447          

HSP 6 Score: 124.79 bits (312), Expect = 3.629e-28
Identity = 94/347 (27.09%), Postives = 162/347 (46.69%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLP-----------EFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            + AA +G+ E     +  G  ++  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++   L+D    S +  + DG T L +A+                    RLP           +    LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D    I      G + L +A+Q  HL    LL+     V  + +   T   VA    H K+  +L
Sbjct:   29 LRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNG-ASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVL 369          
BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Match: sp|Q9ULJ7|ANR50_HUMAN (Ankyrin repeat domain-containing protein 50 OS=Homo sapiens OX=9606 GN=ANKRD50 PE=1 SV=4)

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 393/1038 (37.86%), Postives = 601/1038 (57.90%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDE--NLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE--VKGILPCEKFGSLNDWSIDDIVKILFESINLVN--------INKLNAKKSFTIFS----TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            LA HFC A D D+L +  FI  +   + +S    + Y   +    + +L         P  AFK  +LLPLL   PP +++Y+L+DS+DE  N+     GE+ S              S  ++ LL+ HH FFP WL L+C+ RK+++ + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+  E+Y A+   +++  L   +  L  +   LV    N     H S  EWLLDVK CT KY C+  +GH  LA+     +  L PL+ + FA HLI S    + +    A+W++++   +   +  ++P E+          +++++L ++   VN        I +   ++  +I +       +N   +   ++ A   YS SL         + ++ L+  G D+   + +G T L +AA+ G T + N L+  GAN    D+DGW+ALR A W G+ EVV  LL   +  D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NEIDN GRIP +L+ QEG+    + L++    +++ + YDG  +L++A L    + VE L S GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++ QLLI+ G  +D TC+QGATAL IA+QEGH+++  +L+  GA+  H D   RT  +VA K  H +I  LLEK+GA
Sbjct:  108 LAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLL-QGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITE---GEQTSTSL-----------SGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSN--LQLETAELALWMIWNGTPVRDSLSTLIPKEQ----------EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSL---------DVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLEN-KSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLE-----NGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGA 1102          

HSP 2 Score: 253.447 bits (646), Expect = 2.154e-67
Identity = 177/553 (32.01%), Postives = 288/553 (52.08%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            L+  G  +N  +E+  TS +V            LL +GA+    D +G + L  A ++G ++VV LL+S+  D +     G T L  AA  G T +V  L+  G+NIN  D +G T L +A +     +V  LL  G  +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A         E L+ +GA     DN+GR P  ++  +  YDC Q+LL+     N +NI+    DGR A+ +A+ +G  +I++ L   GA ++ KD +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  +LI + A+V   D+  R+  + A    H+K+  LL +HGA+VD
Sbjct:  500 LVKAGAHVN--SEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLE-----NKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVD 1039          

HSP 3 Score: 191.045 bits (484), Expect = 9.295e-48
Identity = 142/463 (30.67%), Postives = 229/463 (49.46%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC-----QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +++ LL+ +G+++N +D      +  A  + D +  + LL+NGA ++  D++GR+ +  AA      S  LD++ LL+    ++ + D  G   L +AA +G   +   L+   A+I+  D+   + L +AA+ G+ EVV  LL+ G  + C D    + L  +A  G+  I+  LL  G +    DN G T L  A+  G  E V  LL  GA+ N  D  GR  L ++       +G+  +   LID   E V+    DG T L +A      + V+ LL  GAD    DNNGRTPL  +           LL          ++ +D +GRT + IAS +G+ E++++L+D G   + +D+ G T LH+ A++G   IC+ LI+ G   +E  + G     +ASQEGH +   +L+   +N+       R   +VA    H  I  LL  HGA V+ K+ 
Sbjct:  495 EVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSI--RTLLDNGASVNQCDSNGRTLLANAAY-----SGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE-VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAA-----VDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDA 944          

HSP 4 Score: 152.91 bits (385), Expect = 5.930e-36
Identity = 99/303 (32.67%), Postives = 164/303 (54.13%), Query Frame = 2
Query: 1670 QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            Q +   +  L+  GLD N  ++ G T L +AA  G   IC  L+  GA    ID DG     LA   G+ + V++LL  K + D  G DGR ALR AA  G  DIV LL S G+++N +D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      +++++++L+ Y+ ++N  DNE  + L  AA++G   + ++L+E+ A +D       + L  AA  G+ +VV++LL  GA  +  D+ G + + ++A  G+SQI+  L   G   ++    GC+P  + ++E
Sbjct:  824 QGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVS--CWQG---HMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLN----GCSPSPVHTME 1117          
BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.645e-59
Identity = 160/547 (29.25%), Postives = 267/547 (48.81%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    S N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L +A++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   44 IKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 575          

HSP 2 Score: 218.009 bits (554), Expect = 4.821e-56
Identity = 166/618 (26.86%), Postives = 289/618 (46.76%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIF-------VAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     RSA         ++ L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A  +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L +A      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL++  ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  109 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 720          

HSP 3 Score: 199.904 bits (507), Expect = 2.545e-50
Identity = 150/551 (27.22%), Postives = 258/551 (46.82%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   ++ N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L ++A +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G TAL  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 799          

HSP 4 Score: 159.458 bits (402), Expect = 7.385e-38
Identity = 129/465 (27.74%), Postives = 211/465 (45.38%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+      N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G + + +AA        + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG T LH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  345 DVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAA-----REGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALAIARRLGYISVVDTL 798          

HSP 5 Score: 132.88 bits (333), Expect = 1.043e-29
Identity = 105/398 (26.38%), Postives = 185/398 (46.48%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  S       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   + +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   60 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDT----KGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRA-AAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 447          

HSP 6 Score: 124.79 bits (312), Expect = 2.538e-27
Identity = 94/347 (27.09%), Postives = 162/347 (46.69%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLP-----------EFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            + AA +G+ E     +  G  ++  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++   L+D    S +  + DG T L +A+                    RLP           +    LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D    I      G + L +A+Q  HL    LL+     V  + +   T   VA    H K+  +L
Sbjct:   29 LRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNG-ASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVL 369          
BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Match: sp|Q12955|ANK3_HUMAN (Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=3)

HSP 1 Score: 226.868 bits (577), Expect = 1.435e-58
Identity = 160/547 (29.25%), Postives = 268/547 (48.99%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+DIN  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L ++++EGH ++   L++ GA++        TP  VA K   +++ NLL +  A  D
Sbjct:   61 IKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDK-KANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPD 592          

HSP 2 Score: 215.312 bits (547), Expect = 5.672e-55
Identity = 172/664 (25.90%), Postives = 300/664 (45.18%), Query Frame = 2
Query: 1526 INLVNINKLNAKK--SFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKI-----------------------------DPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            IN+ N N LNA    S       +  +    +N   AT   + +L  +        +  L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                                 + D     G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG + +   A                            L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L ++      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL+   ANV   +    TP  +A + + + +  +L   GA VD
Sbjct:   67 INICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQ-QGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASP-----DAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVD 724          

HSP 3 Score: 201.06 bits (510), Expect = 1.396e-50
Identity = 152/551 (27.59%), Postives = 257/551 (46.64%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L +SA +G+  + + LL  G         G TPLH+A+  G+ E  NLLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  277 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLD-QGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALGIARRLGYISVVDTLK 816          

HSP 4 Score: 162.54 bits (410), Expect = 8.972e-39
Identity = 130/465 (27.96%), Postives = 213/465 (45.81%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G +      L L++   + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG TPLH +  Q       VLLQ++ + N   +N     G TA+ IA   G   ++ +L
Sbjct:  362 DVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-----PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAH-VDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALGIARRLGYISVVDTL 815          

HSP 5 Score: 134.035 bits (336), Expect = 4.866e-30
Identity = 108/419 (25.78%), Postives = 196/419 (46.78%), Query Frame = 2
Query: 2096 LNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            + NG  I+  + +G +A+ +A     S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVV+ LL  GA+     + GF+ L+++  QG+ Q++S LL        R       LH+A+ +   +   LLL++    +     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  +       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   61 IKNGVDINICNQNGLNALHLA-----SKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR----LPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNR-AAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 464          

HSP 6 Score: 129.798 bits (325), Expect = 1.066e-28
Identity = 102/387 (26.36%), Postives = 175/387 (45.22%), Query Frame = 2
Query: 2354 MAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLN------------------RLPEF-----------VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLID---------------------------------FGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
            + AA +G+ E     +  G  I+  +++G + L +++ +G+ +++SELL R  +       G T LH+AS+ GQ E V +L+ +GA  N    +G  PL ++ QE ++++ + L+D    S +  + DG T L +A+                    RLP                LL    +A ++  +G TPLHI+      +   +LL  +   +    N +     T +H+AS +G+  ++K L+D GA+ID K  +G T LH GA  G  Q+ ++L+D                                   + +D+  +   TAL +A+  GH ++  +L++  AN         TP  +A K N IK+  LL KHGA +
Sbjct:   46 LRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDN-GASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDI-----TPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASI 426          
BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3)

HSP 1 Score: 221.476 bits (563), Expect = 6.284e-57
Identity = 159/555 (28.65%), Postives = 267/555 (48.11%), Query Frame = 2
Query: 1700 IINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCN--------INLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            I NG+D+N  N+NG  +L +A++ G   + + LL   AN     K G +AL +A   G  EVVK+L++   + +    +G T L  AA     ++V  LL +G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N        +N     G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    + G + L   A  G+ Q++  LL R    + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ L+D    + N K+ +G T L IA        +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E+++ L+  GA+++ K  + +T LH+ A  G   I Q L+  G + +   + G T L ++++EGH ++   L++ GA++        TP  VA K   +++ +LL +  A  D
Sbjct:   61 IKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKK-ANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVR-----GETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPD 600          

HSP 2 Score: 211.075 bits (536), Expect = 9.141e-54
Identity = 165/626 (26.36%), Postives = 291/626 (46.49%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKIDPDFTG----ND-----------------GRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDG---------------------RSAIF-------VAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIG 3370
            L+ NG ++N  ++NG T L +AAQ     +   LL +GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D         ND                 G T L  AA  G  ++  LLL+  + ++       TPL  A+ + +  MVKLLL+ GA ID +  DG                     R+A         ++ L + +   +L+ ++LLL++N  ++ + N+ +  L +AA+ G   + K+LL+  A+ +   +   +PL  A       V+ LLL  GA+I  + +SG + + ++A  G+  I+S+L+  G      +  G T LH+A+  GQ E V  L++ GA+        + PL +S + G   I ++L+     S N  +  G T L ++      +   FLL  GA  +I    G TPLH++    + +   +LLQ S +      +     G T +H+A+   + ++   L+D GA        G T LH+ A +    I   L+++G + +    QG  ++ +A+QEGH+++ +LL++  ANV   +    TP  +A + + + +  +L   GA VD +    +  + +G
Sbjct:  126 LVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQG-ASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSAS-----PDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVG 745          

HSP 3 Score: 202.601 bits (514), Expect = 4.536e-51
Identity = 162/546 (29.67%), Postives = 258/546 (47.25%), Query Frame = 2
Query: 1718 INCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNH---IKICNLLEKHGALVDRK 3337
            +N   E+G T L +AA  G  ++  LLL+  A      ++  + L +A   G   +VKLLL +    D    DG T L   A  G   +V +LL   + I  +   G +PL  AT       V+LLL +   +D   ND  +A+ VAA C      +  + K+LL    N N     G   L IA  +    + ++LL++ A I  V  S   P+  AAF G+  +V  L+  GA+ +  +  G + L ++A  G ++++  L+  G     +     TPLH+++  G+ + V  LL+ GA PN     G  PL LS +EG+  +A  L+D    S++  +  G T L +A      E    LL + A       +G TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   +I  SL++ GA  +    +G   +HL A +G   +  LL+    N++ +   G T L +A+QE  + +  +L+N GA   H+D+  +  Y   + G H   IKI N L +H A V+ K
Sbjct:  236 VNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHC-----GHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHG-ASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVA---AHYDNQKVALL--LLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGA---HVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAK 767          

HSP 4 Score: 200.29 bits (508), Expect = 1.918e-50
Identity = 151/551 (27.40%), Postives = 257/551 (46.64%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            ++ + N +  L+  G  I+    +G T L   A++G   +  +LL   A  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +LL   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA        +++I+  L+ +  + N  +  G   L +AA  G   + + L+++ A ++     D++PL  +A  G  ++V+ LL  GA+ +    SG++ L +SA +G+  + + LL  G         G TPLH+A+  G+ E  +LLL+  A P+     G  PL ++      K+A  L+D    S +  + +G T L IA      +    LL  GADA      G   +H++  +   D   +LL  +      N+N  +  G T +H+A+ +    + + L++ GA +D +   G T LH+G   G  +I   L+     ++     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L+
Sbjct:  285 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFM-----GHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQG-ASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNA-----NVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLK 824          

HSP 5 Score: 160.614 bits (405), Expect = 3.821e-38
Identity = 129/455 (28.35%), Postives = 208/455 (45.71%), Query Frame = 2
Query: 1631 TNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKG 2986
            TN Y  +L  +     Y     L+    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G++ +V  L+     P+ T   G TAL  AA  G+ ++V  L+  G+ +  +  + +TPL  +       +V+ LL  GA  +     G +      L L++   + D+   LL +  ++++   +G   L +AA  G   +  +LL+ +A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G D       G   +HLA+ EG  + V+LLL   A  N  +  G  PL L+ QE  + +AE L++     V+ ++  G T L +       + V FLL   A    +  NG TPLH +  Q       VLLQ++ + N   +N     G TA+ IA   G
Sbjct:  372 TNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYT-----PLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQG-AHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVN-----GNTALAIARRLG 815          

HSP 6 Score: 132.494 bits (332), Expect = 1.464e-29
Identity = 95/348 (27.30%), Postives = 157/348 (45.11%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            S R    + + QL+  G   N    +G T L ++A+ G   +   LL  GA+  +  K G++ L +A   G +EV  LLL +   PD  G  G T L  AA      + LLLL  G++ +     G TPL  A  K    +   LL  GA     D +  +   +A++ L +   ++D++ LLL  N N+NL +  G+  L +AA E    + ++L+   A +D   +   +PL      GN ++V  LL   A ++   K+G++ L  +A QG++ I                                 +N+LL++ A PNE+  +G   L ++ + GYI + + L
Sbjct:  514 SARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGA-----DANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHI---------------------------------INVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 823          

HSP 7 Score: 129.028 bits (323), Expect = 1.764e-28
Identity = 106/406 (26.11%), Postives = 185/406 (45.57%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKP--------NEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   +++++  LL+   N++    +G   L IA+  G   + K+L+ N A+++   +   +PL  AA   + EVVR LL  GA+     + GF+ L+++  QG+ Q++S LL        +       LH+A+ +   +   LLL++            N     G  PL ++   G I +A  L++    +V+F + +  T L +A        V+ LL RGA    +  +G TPLH           ++LL  +       I     +G + +H+A+       ++ L+     +D   N+  T LH+ A  G Y++ ++L+D   N +     G T L IA ++  + +  LL+  GA++  +     TP  VA    H+ I + L  HGA  +  NV
Sbjct:   77 ALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDT----KGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRA-AAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAP-----ILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNV 472          
BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Match: sp|Q5F478|ANR44_CHICK (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Gallus gallus OX=9031 GN=ANKRD44 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 1.317e-55
Identity = 173/606 (28.55%), Postives = 286/606 (47.19%), Query Frame = 2
Query: 1685 TISQ-LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALC-----------LNSNSKYLD------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI-----DIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNF-GCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLID------------------------------VGA----RIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFG-ANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            T SQ LI NG +I+CV+++G T L VAA+ G   + N L++SGA+           L LA  N + +  + LLS   + D   + GRT L  AA  G  + + LL SSG++ NK+D  GRTPL  A   C +  ++ L+  GA I+  D+ GR+ +  AA              + N++ L+             ++ LL+ + N ++ D EG N +  AA  G +   ++LLE N+++         +SPL  AA++G+ + + +LL     +   D+ G + L ++A +G+++ +  L+++G     +DN    TPLH + + G   C+ LLL+    P+  D  G+ PLML+   G+I     L++    SV+     G T+L   ++    E V+ LL +      +D  GRTPLH +  +        LLQ +L+    + +  D+ G T +H A + G    I+ L++                              +GA     ++ KD++GRT LH  A+    +  QLL+     ++     G TAL +A+Q GH+   + L+N   A++   D    T   +A    H K C LL
Sbjct:  320 TRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHNMFPLHLAALNAHSDCCRKLLSSGFEIDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKNILGNSHENAEELERTSEMKEKEAALCLEFLLQNDANPSIQDKEGYNTVHYAAAYGHRQCLELLLEKNSNMFEESDSSATKSPLHLAAYNGHHQALEVLLQSLVDLDIKDEKGRTALDLAAFKGHAECVEALISQGASVTVKDNVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKGQTPLMLAVAYGHIDAVSLLLE-KEASVDAADLLGCTALHRGIMTGHEECVQMLLEKEVSILCKDARGRTPLHFAAARGHATWLSELLQIALSE--EDCSLKDNQGYTPLHWACYNGHENCIEVLLEQKFFRKFYGNSFSPLHCAVINDHENCASMLIGAIDASIVNCKDDKGRTPLHAAAFADHVECLQLLLSHSAQVNAVDHAGKTALMMAAQNGHVGAVDFLVNIAKADLTLRDKDSNTSLHLASSKGHEK-CALL 921          

HSP 2 Score: 211.846 bits (538), Expect = 7.676e-55
Identity = 174/632 (27.53%), Postives = 282/632 (44.62%), Query Frame = 2
Query: 1634 NVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLD----------EMH-----------------------RDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLI---------DVWPESV----------NFKSYDGSTSLKIAVLNRLPEF--------VEFLLSRGADATIQDNNG-----------------------------------RTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDN-EGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANV 3229
            N++   L  ++   S   +  LI +  D+N  ++N  T L VAA N       +++   ++  + D+ G +AL  A  NG++E+V LLL++  + +      R AL  AA++G  ++V LL++ G+ +  +D +G TPL AA       +VK LLN G  ID  +  G +A+ +A  C N       ++  L+ Y  N+N  +N G   L  AA      LC ++L+ N AD++I     +SPL   A  G F   + L+  G  I C+DK G + L ++A  G+  +++ L+  G D           +H                        D+FG T LH A+  G  EC+ LL  SGA  N+ D  GR PL  +    +    E L+         D W  +             K+  G++      L R  E         +EFLL   A+ +IQD  G                                   ++PLH++     +   +VLLQ     ++ +++  D+ GRTA+ +A++KG  E +++LI  GA + +KDN   RT LH     G     +LL++   N D T ++G T L +A   GH++  +LL+   A+V
Sbjct:   72 NMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAEIIIPMLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLEVVALLINHGAEVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIA--CYNGQDS---VVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHNMFPLHLAALNAHSDCCRKLLSSGFEIDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKNILGNSHENAEELERTSEMKEKEAALCLEFLLQNDANPSIQDKEGYNTVHYAAAYGHRQCLELLLEKNSNMFEESDSSATKSPLHLAAYNGHHQALEVLLQ-----SLVDLDIKDEKGRTALDLAAFKGHAECVEALISQGASVTVKDNVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKGQTPLMLAVAYGHIDAVSLLLEKEASV 693          

HSP 3 Score: 181.8 bits (460), Expect = 3.326e-45
Identity = 138/486 (28.40%), Postives = 237/486 (48.77%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            AI++G  E +++L+ +  D +    + RT L  A+++G  DI+ LL+ SG+ +N +D+   TPL  A        V++L+ + A ++ +D + ++ + VAA      +K L   ++++    ++N+ D  G   L  AA  G   +  +LL   A+I   D  DR  L  AA+ G+ EVV LL+  GA + C DK G++ L  +AS G   I+  LL  G++    + +G T LH+A   GQ   VN L+  GA  N+ +N+G  PL  +    +  +  +L+      VN +S DG + L +  ++      + L+  G +    D +G TPLH++   ++Y   ++L+   +T   +   C        +H+A+     +  + L+  G  ID  D+ GRT LH  A  G  +  +LL   G + ++   +G T L  A+   H      L+  GAN+   D   RTP   A   +  +  N+L
Sbjct:   15 AIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDADIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAA-----NKALKCAEIIIPMLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLEVVALLINHGAEVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVA---ARYG-HELLINTLITSGADTAKC-GIHNMFPLHLAALNAHSDCCRKLLSSGFEIDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKNIL 490          

HSP 4 Score: 159.073 bits (401), Expect = 4.501e-38
Identity = 147/512 (28.71%), Postives = 225/512 (43.95%), Query Frame = 2
Query: 1652 LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAW---IGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYE-CVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIAS 3175
            L ++I       I  LI    D+N ++    T L VA+  G   I  LL+ SGA     D    + L  A+ +   E V++L+    D +    + +T L  AA    +   +I++ +LSS   +N  D  GRT L  A       MV LLL  GA I+  D   R A+  AA        +L+++ LL+ +                     G+++ CK         D    +PL AAA +G   +V+ LL  G  I  ++  G + L I+   G   +++EL+  G +    +N G TPLH A+       C+ LL+ +GA  N     G+ PL ++   G    ++ LI    E ++    DG+T L +A        +  L++ GAD      +   PLH++ L +  DC + LL          I+  D  GRT +H A+  G+ E IK L   GA  + KD  GRT LH  A    +   + L+  G NI+ET   G T L  A+
Sbjct:   12 LVQAIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDADIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAEIIIPMLSS---VNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYM-----GHLEVVALLINH---------------------GAEVTCK---------DKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGE-IDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHNMFPLHLAALNAHSDCCRKLLSSGF-----EIDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAA 479          

HSP 5 Score: 151.754 bits (382), Expect = 8.580e-36
Identity = 144/541 (26.62%), Postives = 249/541 (46.03%), Query Frame = 2
Query: 1679 YNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQD--SEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEI---DNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGST--SLKIAVLNRLPEFVEFLLSRGADATI---QDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVG-ARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN----IDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDS 3244
            ++ I  L+  G +IN  ++ G T L  AA +      N+L +S  N   +++      + A        ++ LL    +P     +G   +  AA  G    + LLL   SN+ ++   S  ++PL  A Y   +  +++LL +   +D +D  GR+A+ +AA        + + ++ L+    ++ + DN      L A+       C ++LLE   + D+ D   ++PLM A   G+ + V LLL   A++   D  G + L      G+ + +  LL + +  + +D  G TPLH A+  G    ++ LL+      +    DN G  PL  +C  G+    E  I+V  E   F+ + G++   L  AV+N        L+    DA+I   +D+ GRTPLH +      +C Q+LL HS       +N +D  G+TA+ +A+  G    +  L+++  A + L+D +  T LHL + +G ++ C LLI   I     I+   +   T L IA++ G   +   L+  GA V  +D 
Sbjct:  451 FHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKNILGNSHENAEELERTSEMKEKEAAL-----CLEFLLQNDANPSIQDKEGYNTVHYAAAYGHRQCLELLLEKNSNMFEESDSSATKSPLHLAAYNGHHQALEVLLQSLVDLDIKDEKGRTALDLAAF-----KGHAECVEALISQGASVTVKDNVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKGQTPLMLAVAYGHIDAVSLLLEKEASVDAADLLGCTALHRGIMTGHEECVQMLLEKEVSILCKDARGRTPLHFAAARGHATWLSELLQIALSEEDCSLKDNQGYTPLHWACYNGH----ENCIEVLLEQKFFRKFYGNSFSPLHCAVINDHENCASMLIG-AIDASIVNCKDDKGRTPLHAAAFADHVECLQLLLSHSA-----QVNAVDHAGKTALMMAAQNGHVGAVDFLVNIAKADLTLRDKDSNTSLHLASSKG-HEKCALLILDKIQEQSLINAKNNSLQTPLHIAARNGLKMVVEELLAKGACVLAVDE 970          

HSP 6 Score: 143.28 bits (360), Expect = 3.131e-33
Identity = 93/304 (30.59%), Postives = 151/304 (49.67%), Query Frame = 2
Query: 2321 ADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEIC-NLLINFGANV 3229
            A + + D+ PL+ A FSG+ E +R+L++    ++ +D    + L +++  G++ I+  L+  G     +DN   TPLH A      E V +L+K  A  N  D + + PL ++     +K AE +I +   SVN     G T+L  A LN   E V  LL++GA+    D   R  LH +      +   +L+ H        + C D  G T +H A+  G   I+K L+++G  ID  +  G T LH+  + G   +   LID+G N+++  + G T L  A+   H  +C  LL+N GA+V
Sbjct:    2 AVLKLADQPPLVQAIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDADIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAEIIIPML-SSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLEVVALLINHGA-----EVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGADV 299          

HSP 7 Score: 140.969 bits (354), Expect = 1.577e-32
Identity = 115/438 (26.26%), Postives = 209/438 (47.72%), Query Frame = 2
Query: 2039 RTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYD-CFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            + PL+ A +  D   +++L+     ++  D + R+ + VA+   ++     DII+LL+     +N  DN  +  L  A    S+   ++L++++AD++  D+   +PL  AA +   +   +++   ++++  D+ G + L  +A  G+ ++++ LLA+G +    D      LH A+  G  E V LL+  GA+    D  G  PL  +   G I I + L+++  E      Y G+T+L IA  N     V  L+  GA+    +NNG TPLH +   +    C ++L+      N  ++N    DG++ +H+ +  G     ++LI  G  ID  D +G T LH+ A  G   +   LI  G +  +        L +A+   H + C  L++ G  +   DS  RT    A  G +++   LL+  GA  ++K+
Sbjct:    9 QPPLVQAIFSGDPEEIRMLIYKTEDVNALDAEKRTPLHVASFLGDA-----DIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKALKCAEIIIPMLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLEVVALLINHGAEVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNIY-GNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGALCLELLVN-----NGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHNMFPLHLAALNAHSDCCRKLLSSGFEIDTPDSFGRTCLHAAAAGGNVECIKLLQSSGADFNKKD 435          

HSP 8 Score: 105.145 bits (261), Expect = 1.762e-21
Identity = 114/467 (24.41%), Postives = 189/467 (40.47%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSA----LRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDS-EGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAFSGNFE-VVRLLLFWGAAIHCI--DKSGFSVLSISASQGNSQILSELLAR---------GLDEMH-------------------------RDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLK------------------------------------IAVLNRLPEFVEFLLSRGA-----DATIQDNNG 2839
            L+ N  + +  ++ G  ++  AA  G      LLL   +N  M ++   SA    L LA +NG+ + +++LL   +D D     GRTAL  AA+ G  + V  L+S G+++  +D+   RTPL A+        ++LLL      D  D  G++ + +A         ++D + LLL    +++  D  G   L      G +   ++LLE    I   D   R+PL  AA  G+   +  LL    +   C   D  G++ L  +   G+   +  LL +             +H                         +D+ G TPLH A+     EC+ LLL   A+ N +D+ G+  LM++ Q G++   + L+++    +  +  D +TSL                                     IA  N L   VE LL++GA     D  +  +NG
Sbjct:  518 LLQNDANPSIQDKEGYNTVHYAAAYGHRQCLELLLEKNSN--MFEESDSSATKSPLHLAAYNGHHQALEVLLQSLVDLDIKDEKGRTALDLAAFKGHAECVEALISQGASVTVKDNVTKRTPLHASVINGHTPCLRLLLEVADNPDVTDAKGQTPLMLAVA-----YGHIDAVSLLLEKEASVDAADLLGCTALHRGIMTGHEECVQMLLEKEVSILCKDARGRTPLHFAAARGHATWLSELLQIALSEEDCSLKDNQGYTPLHWACYNGHENCIEVLLEQKFFRKFYGNSFSPLHCAVINDHENCASMLIGAIDASIVNCKDDKGRTPLHAAAFADHVECLQLLLSHSAQVNAVDHAGKTALMMAAQNGHVGAVDFLVNIAKADLTLRDKDSNTSLHLASSKGHEKCALLILDKIQEQSLINAKNNSLQTPLHIAARNGLKMVVEELLAKGACVLAVDENVSRSNG 977          

HSP 9 Score: 90.8929 bits (224), Expect = 4.817e-17
Identity = 65/211 (30.81%), Postives = 99/211 (46.92%), Query Frame = 2
Query: 2711 ESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            E VN    +  T L +A      + +E L+  GA    +DN   TPLH +V     +  QVL++HS      ++N  D + +T +H+A+     +  + +I + + +++ D  GRT LH  A  G  ++  LL+  G NI+    +   AL  A+  GHLE+  LLIN GA V   D    TP   A     I I   L   G  +D  N+
Sbjct:   32 EDVNALDAEKRTPLHVASFLGDADIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSA-----DVNARDKNWQTPLHVAAANKALKCAEIIIPMLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLEVVALLINHGAEVTCKDKKGYTPLHAAASNGQINIVKHLLNLGVEIDEMNI 237          
BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Match: A0A1S3H8J0 (ankyrin repeat domain-containing protein 50 OS=Lingula unguis OX=7574 GN=LOC106153074 PE=4 SV=1)

HSP 1 Score: 747.658 bits (1929), Expect = 0.000e+0
Identity = 414/1068 (38.76%), Postives = 630/1068 (58.99%), Query Frame = 2
Query:  188 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNY-PDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALL--NLDINTDLYYLK-DMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIE---VKGILPCEKFGSLNDWSIDDIVKILFES-INLVNINKLNAKKSFTIFSTYILNITSTYS---------NSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYL--DIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGAL 3325
            K+ +C  +T        + Q L   +LA HFC A DI++L+L  FI  +   + KS    E Y R++ S       L +  C   PD AFK G+L PLLS PPP++N++IL+DSIDE+ L               + +    GSK I++LL+ HH  FP WL L+C+ RK+++ + R F GFRK  LDDL KS+V+RD+Q YIL R+D++  L+Q ++ ET E+LN LHIKSNG  +YLE VLDG+ + ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++   L+++E++ ++   N  +N D ++ + + L KI +   +     +   H+S  EWLLDVK CT KY C+  +GH  LA+     +  L+P ++ N A HL  +  +   +G+H  +WL+ S   +E   + G LP E+           + K+L E+   L    + +   +           + +           N ++AT      L  +  Q +   +  LI  G D+  +++ G T+L +AA+ G + I ++L+ + AN    D +GW+ LR A W G+ EVV LLL      D   ++ RTALR AAW G  DIVL LL  G+++NK D+EGRT L+AA+Y     +V+ LL +GA ++ +D DGR+A+ VAALC+ ++   L   ++ LLL Y   ++  D +GM  +L+AAYEG   +C++LLEN+AD+D  D   R+PL+AAA  G+ +VV LLLF+GAAI  ID  G +VLSI+ +QGN +++ ELL RGLDE+HRDN G TPLH+A+ EG  E    L++ G+K NE DN GRIPL+++ QEG+++    L+      ++ +S+DG  + ++A +    E  E L+  G D    D +GR+ L++  L++       LL+      I      D +GRT +H+ASW+G  EI ++L++ GA ++  DN+ RT L   +WQG + I +L+++FG +++ TC+QGAT+L IA+QEGH EI  LL+ +GAN  H D C RTP +VA KGNH  +C LLE++GA 
Sbjct:   46 KTAVCAEMT--WPTGQGRQQALSKRILAYHFCQAHDIETLSLTNFILSLVDQISKSDLI-EGY-RDVISEPDVKAALKRETCEVNPDDAFKKGVLDPLLSIPPPSQNVFILVDSIDESYLQ--------------SINDTGTGSKTIAELLANHHQLFPTWLMLICSARKQSKSVTRLFTGFRKISLDDLRKSHVVRDVQQYILCRLDQEQGLRQHLSRETAEMLNQLHIKSNGCFLYLEKVLDGVAESFIMLREIREIPGTLNGLYLWLCQRLFVKKQ-FQKVQPILNVILAARSPLTEEELFRSVWTGNTTLNVDEFHSRMEALSKIIFSGQEGTKILF---HHSFAEWLLDVKHCTQKYLCNAAEGHGMLAMNLTLTAPHLSPEEVHNLALHLSKAHLVAPTEGFHLPLWLIQSGAPVETSLMAGGLPKEQ----------QVTKLLLEAGAELPKSAEESTSATLEKIEKQEKKKSESLKELVENGVGINEVDATG--RPLLCAAAHQGNIAMVQLLISRGADLEAMDKTGQTALNLAARQGHSEIVSILIKAQANGDHTDTEGWTPLRSAAWGGHTEVVGLLLIAGAQVDLADSENRTALRAAAWGGHEDIVLKLLEYGADVNKVDNEGRTALIAASYMGHTEIVQHLLEHGANLNQEDCDGRTALSVAALCIPASQGGLHASVVSLLLDYGAEVDHQDKDGMTPVLVAAYEGHHEVCELLLENDADVDHTDNNGRTPLLAAASMGHAKVVSLLLFFGAAIDTIDPEGRTVLSIACAQGNVEVVQELLDRGLDELHRDNAGLTPLHMAAFEGHKEVCEALIEHGSKVNETDNEGRIPLIVAAQEGHLECVRVLLAAG-SLLDHRSHDGKNAFRVAAMEGHKEVTELLIQEGTDMNYCDADGRSTLYLVALENNLVITNALLKGGADPEI-----ADFEGRTPLHVASWQGHHEICEALLNWGANVNSVDNDCRTPLQSASWQGHFSIVKLILEFGADVEHTCNQGATSLCIAAQEGHDEIVRLLLQYGANANHADQCGRTPMRVAMKGNHQGVCKLLEEYGAF 1073          
BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Match: A0A087T2Z3 (Ankyrin repeat domain-containing protein 50 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_04310 PE=4 SV=1)

HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0
Identity = 400/1034 (38.68%), Postives = 610/1034 (58.99%), Query Frame = 2
Query:  239 KTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSD---CDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            K   L   LLA HFC A DI+SL+LA FI  +   L  S      YS  I S  +            PD AFK G+L PLL   PP++  +IL+DS+D+++L   +G+K            N   S++I++LLS +H+ FP WL L+C+ RK+++ + R F GFRK  LDDL KS+V+RD+Q YIL R+D+++ L+Q ++ ET E+LN LHIKSNG  +YLE VLDG+ + ++ ++ I+ IPG L GLYLW+ + LF   Q F  V +I N++L+S K L+++ +Y  +   + N      +  L  +   L++         H+S  EWLLDVK CT KY C+  +GH  +A+     S  L+  +I +FA HL         + +H  +WL++ +    D  +K  +P +             VK+L  +   +         S  +    +  + S  S+  +  +     L  +  +   N + +LI  G ++  +++NG + + +AA+ G   I  LLL++GA+    D DGW+ LR + W G+ EVV +LL      D    D RTALR AAW G  +IV  LL+ G+ +NK D+EGRT L+AA Y     +V+ LL++ A I+H+D+DGR+A+ VAALC+ ++  +  ++ LLL     ++  D +GM  LL+AA+EG   +C++LLEN AD+D  D   R+PL+AAA  G+  VVRLLLFWGAA+  ID  G +VLSI+A+QG+   + +LL RGLDEMHRDN G TPLH A+ EG  E   LLL++GAK  E+DN GRIPL+L+ QEG+  + EKL++  P  V+ +++DG T+L+IA L    E V+ L++ GAD   +D +GR+ L++  L+++ +  + LL         ++   D +GRTA+H+++W+G  ++++ L++ GA ++  DN+ RT L   AWQG   + +LL++    +D  C+QGATAL IA+QEGHLE+  +L+ +GA+  H D   R P +VA KG H  +  LLE++ A
Sbjct:   62 KQSTLSKRLLAYHFCQAHDINSLSLASFINNLISQLTASPLLSG-YSDRIKSPEVEAALQLAECERNPDEAFKKGVLFPLLEIDPPSQPCFILVDSVDQSILG-AVGDK------------NGGVSRSIAELLSNYHHLFPRWLLLICSSRKQSKTVTRMFTGFRKISLDDLRKSHVVRDVQQYILCRLDQEEALRQHLSRETAEMLNQLHIKSNGCFMYLEKVLDGVGENFIMLREIREIPGTLNGLYLWLCQRLFVRKQ-FNKVLSILNVILASRKPLTEEVLYQCVWTRNTNLTWEDYQKRLRLLSKVLIEGKGGTKILFHHSFAEWLLDVKHCTQKYLCNAKEGHGMIAMRYTVASPHLSSEEIIDFALHLSKIPLTSSLQSHHITLWLIHCNLPLADCLMKTSMPKDP----------QTVKLLVSAGAKLPTGDTVKDSSPKVPDDPLEALLSVGSDINQVDSNQRTLLHRASHEGIDNLVLRLIEKGANLEAIDKNGQSPINLAARQGHALIVELLLTAGADPDHADNDGWTPLRSSAWAGHTEVVNVLLEHGAQVDLADADHRTALRAAAWGGHEEIVSRLLAHGACVNKVDNEGRTALIAAAYMGHTEIVQQLLDHDAEINHEDSDGRTALSVAALCVPASEGHARVVSLLLERGTEVDHRDKDGMTPLLVAAFEGHSEVCELLLENEADVDHTDNNGRTPLVAAASMGHPSVVRLLLFWGAAVDTIDAEGRTVLSIAAAQGDPDTVRQLLDRGLDEMHRDNGGWTPLHYAAFEGHTEACELLLEAGAKITEVDNDGRIPLILAAQEGHTNLVEKLLEHTPSMVDCRAHDGKTALRIATLEGQKETVQLLINHGADLNYKDADGRSTLYLLALENRTELAEFLLARGA-----DVEARDLEGRTALHVSAWQGHTDMVELLLNHGADVNAVDNDYRTALQSAAWQGHVSVVRLLVERSAIVDHVCNQGATALCIAAQEGHLEVVKVLLEYGADPSHADQFGRNPIRVASKGGHNHVIRLLEEYVA 1065          

HSP 2 Score: 189.889 bits (481), Expect = 6.160e-45
Identity = 146/464 (31.47%), Postives = 238/464 (51.29%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASV-----EGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN-IDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            D +  LLS GS+IN+ DS  RT L  A+++    +V  L+  GA ++  D +G+S I +AA        +  I++LLL    + +  DN+G   L  +A+ G   +  +LLE+ A +D+ D   R+ L AAA+ G+ E+V  LL  GA ++ +D  G + L  +A  G+++I+ +LL    +  H D+ G T L +A++     EG    V+LLL+ G + +  D  G  PL+++  EG+ ++ E L++   + V+    +G T L  A     P  V  LL  GA     D  GRT L I+  Q   D  + LL   L          D+ G T +H A+++G  E  + L++ GA+I   DN+GR  L L A +G   + + L++   + +D     G TAL IA+ EG  E   LLIN GA++ + D+  R+   +    N  ++   L   GA V+ +++
Sbjct:  488 DPLEALLSVGSDINQVDSNQRTLLHRASHEGIDNLVLRLIEKGANLEAIDKNGQSPINLAA-----RQGHALIVELLLTAGADPDHADNDGWTPLRSSAWAGHTEVVNVLLEHGAQVDLADADHRTALRAAAWGGHEEIVSRLLAHGACVNKVDNEGRTALIAAAYMGHTEIVQQLLDHDAEINHEDSDGRTALSVAALCVPASEGHARVVSLLLERGTEVDHRDKDGMTPLLVAAFEGHSEVCELLLENEAD-VDHTDNNGRTPLVAAASMGHPSVVRLLLFWGAAVDTIDAEGRTVLSIAAAQGDPDTVRQLLDRGLDEMHR-----DNGGWTPLHYAAFEGHTEACELLLEAGAKITEVDNDGRIPLILAAQEGHTNLVEKLLEHTPSMVDCRAHDGKTALRIATLEGQKETVQLLINHGADLNYKDADGRSTLYLLALENRTELAEFLLARGADVEARDL 940          

HSP 3 Score: 153.68 bits (387), Expect = 8.739e-34
Identity = 99/316 (31.33%), Postives = 165/316 (52.22%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLS-SGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDN 2632
            L+  G  ++ ++  G T L +AA  G       LL  G +++  D  GW+ L  A + G+ E  +LLL          NDGR  L  AA  G T++V  LL  + S ++ +  +G+T L  AT +     V+LL+N+GA ++++D DGRS +++ AL         ++ + LL    ++   D EG   L ++A++G   + ++LL + AD++ VD   R+ L +AA+ G+  VVRLL+   A +  +   G + L I+A +G+ +++  LL  G D  H D FG  P+ +AS  G    + LL +  A   E+ N
Sbjct:  762 LLFWGAAVDTIDAEGRTVLSIAAAQGDPDTVRQLLDRGLDEMHRDNGGWTPLHYAAFEGHTEACELLLEAGAKITEVDNDGRIPLILAAQEGHTNLVEKLLEHTPSMVDCRAHDGKTALRIATLEGQKETVQLLINHGADLNYKDADGRSTLYLLAL-----ENRTELAEFLLARGADVEARDLEGRTALHVSAWQGHTDMVELLLNHGADVNAVDNDYRTALQSAAWQGHVSVVRLLVERSAIVDHVCNQGATALCIAAQEGHLEVVKVLLEYGADPSHADQFGRNPIRVASKGGHNHVIRLLEEYVAHLQEVGN 1072          

HSP 4 Score: 84.7297 bits (208), Expect = 1.355e-12
Identity = 69/236 (29.24%), Postives = 113/236 (47.88%), Query Frame = 2
Query: 2639 RIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGAD----ATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDR 3334
            +IPL  S Q  +I +       W    N    D    L    + + P+ V+ L+S GA      T++D++ + P          D  + LL        ++IN +D + RT +H AS +G   ++  LI+ GA ++  D  G++ ++L A QG   I +LL+  G + D   + G T L  ++  GH E+ N+L+  GA V   D+  RT  + A  G H +I + L  HGA V++
Sbjct:  423 KIPLTSSLQSHHITL-------WLIHCNLPLAD---CLMKTSMPKDPQTVKLLVSAGAKLPTGDTVKDSSPKVP---------DDPLEALLSVG-----SDINQVDSNQRTLLHRASHEGIDNLVLRLIEKGANLEAIDKNGQSPINLAARQGHALIVELLLTAGADPDHADNDGWTPLRSSAWAGHTEVVNVLLEHGAQVDLADADHRTALRAAAWGGHEEIVSRLLAHGACVNK 634          
BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Match: L7MGU2 (ANK_REP_REGION domain-containing protein (Fragment) OS=Rhipicephalus pulchellus OX=72859 PE=2 SV=1)

HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0
Identity = 383/1054 (36.34%), Postives = 598/1054 (56.74%), Query Frame = 2
Query:  251 LKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYS-----RNINSSGLCNLFLSQNLC-NYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLL-----SDYLGEKLSYIF------NKDTFHK----NRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINS-ETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFES-INLVNINKLNAKKSFTIFSTYI---LNITSTYSNSIEATNVYSFSLFESIR-QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVW---PESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEK 3313
            L   LLA HFC A D  +L +A F+  + + L + +  G +       R+  +S      L    C  +PD AFK  +L PLL   PP +   +L+DS+DE+LL     S   G    +          +  H       +GS+ I++LL+AH   FP+WL LVC+ R++++ I R F GFRK  LDDL K+ V+RD+Q YIL R+D ++ L+Q + + +T E LN LHIKSNG ++YLE +LDG+   +++++ I+HIPG L GLYLW+ + LF+  Q F+ V+ + +++L++ + L+ +E++  L   +           L  +   LV+  D C    H+S  EWLLDVK CT KY C   +GH  LA+       +L P Q+++ A HL+   P    +  H  +WLLYS   +E      C   GS+       ++++L E+   L   +   + +  +   +     L+       S++  +    +L  +   +     + +L+     +   + NG T L +AA+ G   +  LLL +GAN    D +GW+ LR + W G+ +VV+ LL  K   D    + RTALR A+W G  ++V  LL  G+++N+ D EGRT L+AA Y     +V+ LL+ GA  +H D DGR+A+ VAALC+  +  +L ++ LLL    +++ +DNEGM  LL+AA+EG + +C++LL+  AD+D  D   R+PL AAA  G+ +VV LLLFWGA +  ID  G +VLSI+A+QG+ +++ +LL RGLDE+HRDN G TPLH A++EG  E   LL+++GA+ +E DN GR PL+L+ QEG+ +    ++D     P  V+ +++DG T+ ++A L    E V  LLS  AD   QD +GR+ L++  L+ + D    +L       I ++     +GRT +H+A+W+G  ++++ L+  GA +D +D++ RT L   AWQG   + +LL++ G  +D  C +GATAL IASQEGH  +   L+  GA+  H D C R+P +VA K  H+ +  LLE+
Sbjct:  114 LSRRLLAHHFCQAHDTRTLAVASFVLGLVRQLCRCEQAGLLSGYQDRLRDAEAS------LEPVACARWPDEAFKKAVLFPLLEIEPPEQPCLLLVDSVDESLLVQVNASGVTGSHQHHRHHHHHHDGGERRHHCLPDGGRGSRTIAELLAAHQQLFPQWLLLVCSARRQSKTITRMFTGFRKISLDDLRKASVVRDMQQYILARLDGEEALRQQLGARDTAEALNQLHIKSNGCLLYLECLLDGVAQRWVTLREIRHIPGTLNGLYLWLCQRLFQRRQ-FLRVQPVLHVLLAAAQPLTAEELFLCLWTRNQLLTHDEFGRRLALLSRLLVEREDGCLLLFHHSFAEWLLDVKHCTQKYLCHAAEGHAMLAMMQTLRGPQLPPTQVQDLAHHLLRLPPCDAVEPQHLTLWLLYSGVPLECAF---CPTVGSVVIPRDPAVLRLLLEAGARLPTASVAGSDEGGSPVPSMPPDPLDALLGSGASVDDADANQRALLHTAAYEGDAALVERLLHAKASLELADRNGQTPLNLAARQGHADVVVLLLKAGANPDHADNEGWTPLRSSAWAGHGQVVEALLENKAQVDLADGEQRTALRAASWGGHEEVVAQLLEKGADVNRADREGRTALIAAAYMGHAEIVERLLDAGAEANHADGDGRTALSVAALCVRPSEGHLGVVALLLERGADVDHVDNEGMTPLLVAAFEGHREVCELLLDAEADLDHADHSGRTPLFAAASMGHADVVGLLLFWGAYVDSIDAEGRTVLSIAAAQGSVEVVQQLLNRGLDELHRDNAGWTPLHYAALEGHAEVCTLLMEAGAQASETDNEGRTPLILAAQEGHTQAVRAMLDFGGHPPSLVDHRAHDGRTAFRVAALEGHKETVHVLLSYNADVNYQDADGRSTLYVLALEGRVDMADYILARGADPEIGDL-----EGRTPLHVAAWQGHVDLVELLLSRGADVDAEDSDQRTALQSAAWQGQAHVVKLLLERGAQVDHICVEGATALGIASQEGHEAVVRALLEHGADPSHADQCGRSPLRVATKAGHLNVVRLLEE 1152          

HSP 2 Score: 154.451 bits (389), Expect = 5.481e-34
Identity = 102/326 (31.29%), Postives = 160/326 (49.08%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSN----INKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKS 2605
             LF +      + +  L+  G  ++ ++  G T L +AA  G   +   LL+ G ++L  D  GW+ L  A   G+ EV  LL+        T N+GRT L  AA  G T  V  +L  G +    ++ +  +GRT    A  +     V +LL+  A +++QD DGRS ++V AL        +D+   +L    +  + D EG   L +AA++G   L ++LL   AD+D  D   R+ L +AA+ G   VV+LLL  GA +  I   G + L I++ +G+  ++  LL  G D  H D  G +PL +A+  G    V LL +S
Sbjct:  833 PLFAAASMGHADVVGLLLFWGAYVDSIDAEGRTVLSIAAAQGSVEVVQQLLNRGLDELHRDNAGWTPLHYAALEGHAEVCTLLMEAGAQASETDNEGRTPLILAAQEGHTQAVRAMLDFGGHPPSLVDHRAHDGRTAFRVAALEGHKETVHVLLSYNADVNYQDADGRSTLYVLAL-----EGRVDMADYILARGADPEIGDLEGRTPLHVAAWQGHVDLVELLLSRGADVDAEDSDQRTALQSAAWQGQAHVVKLLLERGAQVDHICVEGATALGIASQEGHEAVVRALLEHGADPSHADQCGRSPLRVATKAGHLNVVRLLEES 1153          

HSP 3 Score: 72.0182 bits (175), Expect = 1.064e-8
Identity = 53/193 (27.46%), Postives = 86/193 (44.56%), Query Frame = 2
Query: 2762 VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            V+ R P  +  LL  GA        G       V     D    LL    +     ++  D + R  +H A+++GD  +++ L+   A ++L D  G+T L+L A QG   +  LL+  G N D   ++G T L  ++  GH ++   L+   A V   D   RT  + A  G H ++   L + GA V+R +
Sbjct:  536 VIPRDPAVLRLLLEAGARLPTASVAGSDEGGSPVPSMPPDPLDALLGSGAS-----VDDADANQRALLHTAAYEGDAALVERLLHAKASLELADRNGQTPLNLAARQGHADVVVLLLKAGANPDHADNEGWTPLRSSAWAGHGQVVEALLENKAQVDLADGEQRTALRAASWGGHEEVVAQLLEKGADVNRAD 723          
BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Match: A0A131YW74 (Ankyrin repeat domain-containing protein 50 OS=Rhipicephalus appendiculatus OX=34631 PE=4 SV=1)

HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0
Identity = 383/1055 (36.30%), Postives = 598/1055 (56.68%), Query Frame = 2
Query:  251 LKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYS-----RNINSSGLCNLFLSQNLC-NYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLL-----SDYLGEKLSYIF--------NKDTFH---KNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINS-ETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFES-INLVNINKLNAKKSFTIFSTYI---LNITSTYSNSIEATNVYSFSLFESIR-QNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVW---PESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEK 3313
            L   LLA HFC A D  +L +A F+  + + L + +  G +       R+  +S      L    C  +PD AFK  +L PLL   PP +   +L+DS+DE+LL     S   G    +           +   H      +GS+ I++LL+AH   FP+WL LVC+ R++++ I R F GFRK  LDDL K+ V+RD+Q YIL R+D ++ L+Q + + +T E LN LHIKSNG ++YLE +LDG+   +++++ I+HIPG L GLYLW+ + LF+  Q F+ V+ + +++L++ + L+ +E++  L   +           L  +   LV+  D C    H+S  EWLLDVK CT KY C   +GH  LA+       +L P Q+++ A HL+   P    +  H  +WLLYS   +E      C   GS+       ++++L E+   L   +   + +  +   +     L+       S++  +    +L  +   +     + +L+     +   + NG T L +AA+ G   +  LLL +GAN    D +GW+ LR + W G+ +VV+ LL  K   D    + RTALR A+W G  ++V  LL  G+++N+ D EGRT L+AA Y     +V+ LL+ GA  +H D DGR+A+ VAALC+  +  +L ++ LLL    +++ +DNEGM  LL+AA+EG + +C++LL+  AD+D  D   R+PL AAA  G+ +VV LLLFWGA +  ID  G +VLSI+A+QG+ +++ +LL RGLDE+HRDN G TPLH A++EG  E   LL+++GA+ +E DN GR PL+L+ QEG+ +    ++D     P  V+ +++DG T+ ++A L    E V  LLS  AD   QD +GR+ L++  L+ + D    +L       I ++     +GRT +H+A+W+G  ++++ L+  GA +D +D++ RT L   AWQG   + +LL++ G  +D  C +GATAL IASQEGH  +   L+  GA+  H D C R+P +VA K  H+ +  LLE+
Sbjct:   81 LSRRLLAHHFCQAHDTRTLAVASFVLGLVRQLCRCEQAGLLSGYQDRLRDAEAS------LEPVACARWPDEAFKKAVLFPLLEIEPPEQPCLLLVDSVDESLLVQVNTSGVTGSHQHHRHHHHHHHDGGERRHHCLPDGGRGSRTIAELLAAHQQLFPQWLLLVCSARRQSKTITRMFTGFRKISLDDLRKASVVRDMQQYILARLDGEEALRQQLGARDTAEALNQLHIKSNGCLLYLECLLDGVAQRWVTLREIRHIPGTLNGLYLWLCQRLFQRRQ-FLRVQPVLHVLLAAAQPLTAEELFLCLWTRNQLLTHDEFGRRLALLSRLLVEREDGCLLLFHHSFAEWLLDVKHCTQKYLCHAAEGHAMLAMMQTLRGPQLPPTQVQDLAHHLLRLPPCDAVEPQHLTLWLLYSGVPLECAF---CPTVGSVVIPRDPAVLRLLLEAGARLPTASVAGSDEGGSPVPSMPPDPLDALLGSGASVDDADANQRALLHTAAYEGDAALVERLLHAKASLELADRNGQTPLNLAARQGHADVVVLLLKAGANPDHADNEGWTPLRSSAWAGHGQVVEALLENKAQVDLADGEQRTALRAASWGGHEEVVAQLLEKGADVNRADREGRTALIAAAYMGHAEIVERLLDAGAEANHADGDGRTALSVAALCVRPSEGHLGVVALLLERGADVDHVDNEGMTPLLVAAFEGHREVCELLLDAEADLDHADHSGRTPLFAAASMGHADVVGLLLFWGAYVDSIDAEGRTVLSIAAAQGSVEVVQQLLNRGLDELHRDNAGWTPLHYAALEGHAEVCTLLMEAGAQASETDNEGRTPLILAAQEGHTQAVRAMLDFGGHPPSLVDHRAHDGRTAFRVAALEGHKETVHVLLSYNADVNYQDADGRSTLYVLALEGRVDMADYILARGADPEIGDL-----EGRTPLHVAAWQGHVDLVELLLSRGADVDAEDSDQRTALQSAAWQGQAHVVKLLLERGAQVDHICVEGATALGIASQEGHEAVVRALLEHGADPSHADQCGRSPLRVATKAGHLNVVRLLEE 1120          

HSP 2 Score: 154.066 bits (388), Expect = 6.447e-34
Identity = 102/326 (31.29%), Postives = 160/326 (49.08%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSN----INKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKS 2605
             LF +      + +  L+  G  ++ ++  G T L +AA  G   +   LL+ G ++L  D  GW+ L  A   G+ EV  LL+        T N+GRT L  AA  G T  V  +L  G +    ++ +  +GRT    A  +     V +LL+  A +++QD DGRS ++V AL        +D+   +L    +  + D EG   L +AA++G   L ++LL   AD+D  D   R+ L +AA+ G   VV+LLL  GA +  I   G + L I++ +G+  ++  LL  G D  H D  G +PL +A+  G    V LL +S
Sbjct:  801 PLFAAASMGHADVVGLLLFWGAYVDSIDAEGRTVLSIAAAQGSVEVVQQLLNRGLDELHRDNAGWTPLHYAALEGHAEVCTLLMEAGAQASETDNEGRTPLILAAQEGHTQAVRAMLDFGGHPPSLVDHRAHDGRTAFRVAALEGHKETVHVLLSYNADVNYQDADGRSTLYVLAL-----EGRVDMADYILARGADPEIGDLEGRTPLHVAAWQGHVDLVELLLSRGADVDAEDSDQRTALQSAAWQGQAHVVKLLLERGAQVDHICVEGATALGIASQEGHEAVVRALLEHGADPSHADQCGRSPLRVATKAGHLNVVRLLEES 1121          

HSP 3 Score: 72.0182 bits (175), Expect = 9.543e-9
Identity = 53/193 (27.46%), Postives = 86/193 (44.56%), Query Frame = 2
Query: 2762 VLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            V+ R P  +  LL  GA        G       V     D    LL    +     ++  D + R  +H A+++GD  +++ L+   A ++L D  G+T L+L A QG   +  LL+  G N D   ++G T L  ++  GH ++   L+   A V   D   RT  + A  G H ++   L + GA V+R +
Sbjct:  504 VIPRDPAVLRLLLEAGARLPTASVAGSDEGGSPVPSMPPDPLDALLGSGAS-----VDDADANQRALLHTAAYEGDAALVERLLHAKASLELADRNGQTPLNLAARQGHADVVVLLLKAGANPDHADNEGWTPLRSSAWAGHGQVVEALLENKAQVDLADGEQRTALRAASWGGHEEVVAQLLEKGADVNRAD 691          
BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Match: A0A1S3FLW4 (ankyrin repeat domain-containing protein 50 OS=Dipodomys ordii OX=10020 GN=Ankrd50 PE=4 SV=1)

HSP 1 Score: 712.99 bits (1839), Expect = 0.000e+0
Identity = 393/1036 (37.93%), Postives = 601/1036 (58.01%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDI--EVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNA--------KKSFTIFS----TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            LA HFC A D D+L +  FI  +   + +S    + Y   +    + +L         P  AFK  +LLPLL   PP +++Y+L+DS+DE   +   GE+ S              S  +++LL+ HH+FFP WL L+C+ RK+++ + + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I N++L++ + L+  E+Y A+   +++  L   +  L  +   LV    N     H S  EWLLDVK CT KY C+  +GH  LA+     +  L PL+ + FA HLI S    + +    A+W++++   +   +  ++P E+          +++++L ++   VN     A        ++  +I +       +N   +   ++ A   YS +L         + ++ L+  G D+   + +G T L +AA+ G T + N L+  GAN    D+DGW+ALR A W G+ EVV  LL   +  D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA +DH+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D +GM  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG       L++ GA+ NE DN GRIP +L+ QEG+    + L++    +V+ + YDG  +L++A L    + VE LLS GAD   +D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++CQLLI+ G  +D TC+QGATAL IA+QEGH+++  +L+   A+  H D   RT  +VA K  H +I  LLEK+GA
Sbjct:  108 LAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLL-QGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGW-NIAEGEQTSSSL-----------SGTVAELLAGHHDFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNVILAACRPLTVTELYHAVWTKNMSLTLEDFQRKLDVLSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCRAKNLTPLEAQEFALHLIHSN--LQLEPAELALWMIWNGTPVGDSLSTLIPKEQ----------EVLQLLVKAGAHVNSEDERASCIVRQALEREDSIRTLLDNGASVNQCDSSGRTLLANAAYSGNL---------DVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGRGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAKVDHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDHNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNETDNDGRIPFILASQEGHYDCVQILLES-KSNVDQRGYDGRNALRVAALEGHRDIVELLLSHGADVDYKDADGRPTLYILALENQLTMAEYFLE-----NGANVEASDAEGRTALHVSCWQGHVEMVQVLIACHADVNAADNEKRSALQSAAWQGHVRVCQLLIEHGAQVDHTCNQGATALCIAAQEGHVDVVQVLLEHAADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGA 1102          

HSP 2 Score: 250.366 bits (638), Expect = 7.403e-64
Identity = 169/520 (32.50%), Postives = 274/520 (52.69%), Query Frame = 2
Query: 1796 LLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            LL +GA+    D  G + L  A ++G ++VV LL+S+  D +     G T L  AA  G T +V  L+  G+NIN  D +G T L +A +     +V  LL  G  +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A +D  D   R+ L  AA       G+  VV LL+  GA +   DK G + L ++A +G+  ++  LL  G D  H D+ G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A         E L+ +GA     DN+GR P  ++  +  YDC Q+LL+     + +N++    DGR A+ +A+ +G  +I++ L+  GA +D KD +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  +LI   A+V   D+  R+  + A    H+++C LL +HGA VD
Sbjct:  531 LLDNGASVNQCDSSGRTLLANAAYSGNLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGRGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAKVDHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDHNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNETDNDGRIPFILASQEGHYDCVQILLE-----SKSNVDQRGYDGRNALRVAALEGHRDIVELLLSHGADVDYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHVEMVQVLIACHADVNAADNEKRSALQSAAWQGHVRVCQLLIEHGAQVD 1039          

HSP 3 Score: 196.823 bits (499), Expect = 3.875e-47
Identity = 144/463 (31.10%), Postives = 229/463 (49.46%), Query Frame = 2
Query: 1979 DIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC-----QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +++ LL+ +G+++N +D      +  A  + D +  + LL+NGA ++  D+ GR+ +  AA   N     LD++ LL+    ++ + D  G   L +AA +G   +   L+   A+I+  D+   + L +AA+ G+ EVV  LL+ G  + C D    + L  +A  G+  I+  LL  G +    DN G T L  A+  G  E V  LL  GAK +  D  GR  L ++       +G+  +   LID   E V+    DG T L +A      + V+ LL  GAD    D+NGRTPL  +           LL          ++ +D +GRT + IAS +G+ E++++L+D G   + +D+ G T LH+ A++G   IC+ LI+ G   +ET + G     +ASQEGH +   +L+   +NV       R   +VA    H  I  LL  HGA VD K+ 
Sbjct:  495 EVLQLLVKAGAHVNSEDERASCIVRQALEREDSI--RTLLDNGASVNQCDSSGRTLLANAAYSGN-----LDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGRGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAKVDHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAE-VDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDHNGRTPLLAAASMGHASVVNTLLFWGAA-----VDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNETDNDGRIPFILASQEGHYDCVQILLESKSNVDQRGYDGRNALRVAALEGHRDIVELLLSHGADVDYKDA 944          

HSP 4 Score: 150.599 bits (379), Expect = 8.801e-33
Identity = 104/365 (28.49%), Postives = 187/365 (51.23%), Query Frame = 2
Query: 1499 DIVKILFESINLVNINKLNAKKSF----TIFSTYILNITSTYSNSIEATNVYSFSLFE-SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            D+V +L E    V+    N +       ++    ++N    +  ++++ +    ++   +  Q +   +  L+  GLD N  ++ G T L +AA  G   IC  L+  GA     D DG     LA   G+ + V++LL  K + D  G DGR ALR AA  G  DIV LLLS G++++ +D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      +++++++L+  + ++N  DNE  + L  AA++G   +C++L+E+ A +D       + L  AA  G+ +VV++LL   A  +  D+ G + + ++A  G+SQI+  L   G   ++    GC+P  + ++E
Sbjct:  762 DVVDLLLEGGADVDHTDHNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNETDNDGRIPFILASQEGHYDCVQILLESKSNVDQRGYDGRNALRVAALEGHRDIVELLLSHGADVDYKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVS--CWQG---HVEMVQVLIACHADVNAADNEKRSALQSAAWQGHVRVCQLLIEHGAQVDHTCNQGATALCIAAQEGHVDVVQVLLEHAADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASTLN----GCSPSPVHTME 1117          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Match: ANKRD50 (ankyrin repeat domain 50 [Source:NCBI gene;Acc:103026486])

HSP 1 Score: 686.411 bits (1770), Expect = 0.000e+0
Identity = 391/1046 (37.38%), Postives = 602/1046 (57.55%), Query Frame = 2
Query:  245 QKLKSLLLASHFCSASDIDSLNLAKFI----FEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNY-PDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVN----------INKLNAKKSFTIF--STYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            + L  L LA HFC A D D++ L+ F+     +I    L  +Y  +I    + S+      L    C   P  AFK  +LLPLLS  PP + +++L+DS+DE + +   GE  S   +           ++I +LL+AHH   P WL L+C+ R++N+ I + F GFRK  LDDL K+Y+++D+Q YIL+R+D+++ L+Q +  ET E+LN LHIKS+G  +YLE VLDG+++ ++ ++ I+ IPG L GLYLW+ + LF   Q F  V+ I NI+L++ + L+ +E+Y A+   ++   +   +  +  +   LV          H S  EWLLDVK CT KY C+  +GH  +A+     + +L PL+++ FA HLI S    + + ++ A+W++++          P +   S +     +++++L ++   +N             L  + S      +   +N + +   ++     YS +L         + ++ LI  G ++   + +G T L +AA+ G T + N L+   +N    D DGW+ALR A W G+ EVV  LL      D    D RTALR AAW G  DIVL LL  G+ +NK D+EGRT L+AA Y     +V+ LL++GA ++H+D DGR+A+ VAALC+ ++  +  ++ LL+     ++  D + M  LL+AAYEG   +  +LLE  AD+D  D   R+PL+AAA  G+  VV  LLFWGAA+  ID  G +VLSI+++QGN +++  LL RGLDE HRD+ G TPLH+A+ EG  +  + LL+ GA+ +E+DN GRIPL+L+ QEG+    + L++     ++ + YDG  SL++A L    + VE LLS GAD    D +GR  L+I  L++Q    +  L+     N  N+   D +GRTA+H++ W+G  E+++ LI   A ++  DNE R+ L   AWQG  ++ Q LID G ++D TC+QGATAL IA+QEGH+++  +L+  GA+  H D   RT  +VA KG H  I  LLEK+GA
Sbjct:   94 RGLHQLCLAFHFCRAEDADTVCLSGFVRGLVAQIRAGGLVPQYEEKIRDPAVQSA------LQPGECERNPSEAFKRCVLLPLLSCKPPPQALFLLVDSVDEGVKAAE-GESRSSASS----------PRSIGELLAAHHELLPPWLLLICSGRRQNKNITKLFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQ-FAKVQPILNIILAACRPLTVKELYHAVWTKNMTLTMEEFQKKMDILTKLLVDGLGGTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMMAMSYTCRAKQLKPLEVQEFALHLINSN--LQIEPFNLALWMVWNGT--------PAKDSLSTSIPKEQEVLQLLVKAGAHINNEDDHASCIVQQALEREDSIRTLLDNGASVNQSDSSGRTLLGNAAYSGNL---------DVVNLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLIGCDSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRQVCDALLEQGARCSEVDNDGRIPLILAAQEGHYDCVQILLEN-KSCIDQRGYDGRNSLRVAALEGHRDIVELLLSHGADIDYNDADGRPTLYILALENQLAMAEYFLE-----NGANVETSDTEGRTALHVSCWQGHVEMVRLLISYHADVNSCDNEKRSALQSAAWQGHSKVVQFLIDSGTHVDHTCNQGATALGIAAQEGHIDVVRILLEHGADPNHADQFGRTAMRVAAKGGHSGIIKLLEKYGA 1096          

HSP 2 Score: 243.047 bits (619), Expect = 4.797e-65
Identity = 177/556 (31.83%), Postives = 285/556 (51.26%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAF-----SGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDD---DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            L+  G  IN  NE+   S +V            LL +GA+    D  G + L  A ++G ++VV LL+S+  + +   N G+T L  AA  G T +V  L+   SNIN  D +G T L +A +     +V  LL  GA +D  D D R+A+  AA        + DI+  LL++   +N  DNEG   L+ AAY G + + + LL++ A++   D+  R+ L  AA       G+  VV LL+  GA +   DK   + L ++A +G+  ++  LL  G D  H DN G TPL  A+  G    VN LL  GA  + ID+ GR  L ++  +G +++   L+D   +  N +   G T L +A      +  + LL +GA  +  DN+GR PL ++  +  YDC Q+LL+        N +C+D    DGR ++ +A+ +G  +I++ L+  GA ID  D +GR  L++ A +    + +  ++ G N++ + ++G TAL ++  +GH+E+  LLI++ A+V   D+  R+  + A    H K+   L   G  VD
Sbjct:  494 LVKAGAHIN--NEDDHASCIVQQALEREDSIRTLLDNGASVNQSDSSGRTLLGNAAYSGNLDVVNLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLIGCDSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAW-----GGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDE-NHRDDAGWTPLHMAAFEGHRQVCDALLEQGARCSEVDNDGRIPLILAAQEGHYDCVQILLE--------NKSCIDQRGYDGRNSLRVAALEGHRDIVELLLSHGADIDYNDADGRPTLYILALENQLAMAEYFLENGANVETSDTEGRTALHVSCWQGHVEMVRLLISYHADVNSCDNEKRSALQSAAWQGHSKVVQFLIDSGTHVD 1033          

HSP 3 Score: 169.474 bits (428), Expect = 4.745e-42
Identity = 126/428 (29.44%), Postives = 222/428 (51.87%), Query Frame = 2
Query: 2081 MVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVL-----NRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            +++LL+  GA+I+++D D  S I   AL      +  D I+ LL    ++N  D+ G  +L  AAY G+  +  +L+   A++++ D   ++PL  AA  G+ +VV  L+   + I+  D  G++ L  +A  G+S+++S LL  G      D    T L  A+  G  + V  LL+ GA+ N+ DN GR  L+ +   G+ +I E L+D   E VN +  DG T+L +A L           V  L+ RGA+    D +  TPL ++  +   D   +LL+        +++  D++GRT +  A+  G   ++ +L+  GA +D  D+EGRT L + + QG  ++ + L+D G++ +     G T L +A+ EGH ++C+ L+  GA    +D+  R P  +A +  H     +L ++ + +D++ 
Sbjct:  490 VLQLLVKAGAHINNED-DHASCIVQQAL------EREDSIRTLLDNGASVNQSDSSGRTLLGNAAYSGNLDVVNLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLIGCDSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAE-VNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGA-----DVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRQVCDALLEQGARCSEVDNDGRIPLILAAQEGHYDCVQILLENKSCIDQRG 904          

HSP 4 Score: 149.828 bits (377), Expect = 4.938e-36
Identity = 105/365 (28.77%), Postives = 185/365 (50.68%), Query Frame = 2
Query: 1499 DIVKILFESINLVNINKLNAKKSF----TIFSTYILNITSTYSNSIEATNVYSFSLFE-SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE 2569
            D+V +L E    V+    N +       ++    ++N    +  ++++ +    ++   +  Q +   +  L+  GLD N  ++ G T L +AA  G   +C+ LL  GA    +D DG   L LA   G+ + V++LL  K   D  G DGR +LR AA  G  DIV LLLS G++I+  D++GR  L     +    M +  L NGA ++  D +GR+A+ V+  C      ++++++LL+ Y+ ++N  DNE  + L  AA++G   + + L+++   +D       + L  AA  G+ +VVR+LL  GA  +  D+ G + + ++A  G+S I+  L   G   ++    GC P  + ++E
Sbjct:  756 DVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRQVCDALLEQGARCSEVDNDGRIPLILAAQEGHYDCVQILLENKSCIDQRGYDGRNSLRVAALEGHRDIVELLLSHGADIDYNDADGRPTLYILALENQLAMAEYFLENGANVETSDTEGRTALHVS--CWQG---HVEMVRLLISYHADVNSCDNEKRSALQSAAWQGHSKVVQFLIDSGTHVDHTCNQGATALGIAAQEGHIDVVRILLEHGADPNHADQFGRTAMRVAAKGGHSGIIKLLEKYGASSLN----GCNPSPIHTME 1111          

HSP 5 Score: 119.013 bits (297), Expect = 1.649e-26
Identity = 92/296 (31.08%), Postives = 141/296 (47.64%), Query Frame = 2
Query: 2561 SVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQ-EGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINN------------------------------INCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIA-----SQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            S+  + E + LL+K+GA  N  D+H    +  + + E  I+    L+D    SVN     G T L  A  +   + V  L+SRGA+  +QDN+G+TPL ++  Q        L+      NIN+                              ++C D D RTA+  A+W G  +I+ +L+  GA ++  DNEGRT L   A+ G  +I + L+D G  ++     G TALS+A     + +GH  + +LLI+ GA V H D    TP  VA    H+ + +LL + GA VD  +
Sbjct:  483 SIPKEQEVLQLLVKAGAHINNEDDHASCIVQQALEREDSIRT---LLDNG-ASVNQSDSSGRTLLGNAAYSGNLDVVNLLISRGANMELQDNHGQTPLTLAARQGHTKVVNCLI--GCDSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDCMTPLLVAAYEGHVDVVDLLLEGGADVDHTD 772          

HSP 6 Score: 71.2478 bits (173), Expect = 6.425e-12
Identity = 53/186 (28.49%), Postives = 86/186 (46.24%), Query Frame = 2
Query: 2786 VEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +  LL  GA     D++GRT L  +      D   +L+         N+   D+ G+T + +A+ +G  +++  LI   + I+  D++G T L   AW G  ++   L+  G  +D   +   TAL  A+  GH +I   L+  GA V   D+  RT    A    H +I   L  HGA V+ ++V
Sbjct:  522 IRTLLDNGASVNQSDSSGRTLLGNAAYSGNLDVVNLLISRGA-----NMELQDNHGQTPLTLAARQGHTKVVNCLIGCDSNINHTDHDGWTALRSAAWGGHSEVVSALLYAGAKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDV 702          

HSP 7 Score: 63.1586 bits (152), Expect = 2.038e-9
Identity = 56/209 (26.79%), Postives = 94/209 (44.98%), Query Frame = 2
Query: 1499 DIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTY----SNSIEATNVYS-FSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSG-SNINK------QDSEGRTPLMAATYKCDYVMVK 2089
            DIV++L      ++ N  + + +  I +       + Y      ++E ++     +L  S  Q     +  LI    D+N  +    ++L  AA  G + +   L+ SG +       G +AL +A   G+I+VV++LL    DP+     GRTA+R AA  G + I+ LL   G S++N          E +TPL A T K   + +K
Sbjct:  921 DIVELLLSHGADIDYNDADGRPTLYILALENQLAMAEYFLENGANVETSDTEGRTALHVSCWQGHVEMVRLLISYHADVNSCDNEKRSALQSAAWQGHSKVVQFLIDSGTHVDHTCNQGATALGIAAQEGHIDVVRILLEHGADPNHADQFGRTAMRVAAKGGHSGIIKLLEKYGASSLNGCNPSPIHTMEQKTPL-AVTGKMQSLTIK 1128          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Match: ankrd50l (ankyrin repeat domain-containing protein 50-like [Source:NCBI gene;Acc:103038240])

HSP 1 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 367/1091 (33.64%), Postives = 577/1091 (52.89%), Query Frame = 2
Query:  188 KSTICRLLTTELTIDCFKTQK---LKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSI--DDIVKILFES----------------INLVNINKLNAKKSFTIFSTYILNITSTYS-NSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLM-----------IDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS---KYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID------IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSG--AKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDF-GINIDETC-SQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            K+ +C    TEL     +T K   L S  LA H+C   D  ++ + +F+  + + L  S      YS  + S  +       +    PD  FK  +L PLLS P P + +YI++DS++       +G                  S +I++LL  H   FP W+ LVC+ R++N+ + + F GFRK  LDDL K   +RD+Q YIL R+D++  L++ +  ET ++LNLLHIKS G  ++LE VLDG+  G + ++ I+ IPG L GLYLW+ + LF   + F+ V+ + NI+L+S + L+ +++Y A    D        + ++  +   +V+  +      H S  EW+ DVK CT K+ CS+ DGH++LA+  +  S  L+   I     HL++S  I        A+W++++     V  +  C K  S     +   D++++L +S                I+ V   +   +++     +    + S  S N  + ++  +  L  +      + ++ L+  G D +  +  G T+L + A+ G   +  +LL    +Q              D DGW+ALR A W G+ E VK+LL    D D    DGRTALR AAW G  ++++ LL  G+ ++  D EGRTPL+AA Y      V+++L+ GA +D  D DGR+A+ VAALC+ S +    + +++ LLL  +      D +GM  LL+A+YEG + + ++LLE  AD+D          +PL+AAA  G+   V  +LFWGAA+  ID  G + L ++A++G+ +++  LL RGLDE H+D+ G TPLH A+ +G      +L + G  A+  E+D  GR  L+L+ QEG+      L+D     ++ +SYDG ++L  A L    E VE LL RGAD  ++D  GR  L++ +L+ + D   +L++         +   D +GRTA+H+A+W+GD + I  L+  GA  +  D EGR  LH  AW+G     +L +    IN+D+ C  QGATALSIA+QEGH+EI  +L+  GA+  H+D   RTP KVA K     I  LLE +GA
Sbjct:   57 KTALC----TELVWPQSETGKALGLASRCLAWHYCQQEDAGTMEVWRFVLGLVEQLRDSPLLAPSYSHLLASPAIAASLEPIHCQRDPDDTFKRAVLEPLLSLPSPAQAVYIVVDSLNAGY-GGCVGATEGVTSGSMAIQ-----STSIAELLLRHLQLFPPWILLVCSARRQNKAVCKMFSGFRKLCLDDLRKPAAVRDVQHYILRRLDQEGALRRQLTPETADMLNLLHIKSGGCFLFLEQVLDGVACGLVGLREIRDIPGTLNGLYLWLCQRLFPR-RLFVHVRPLLNILLASPRPLTPEKLYFAAYARDNCLGHEDFQSLMRALAPLIVEGPEGSKLLFHGSFAEWITDVKYCTQKFLCSVKDGHLSLAMSLSLRSKTLSTEDICQLGHHLLSSG-IHAGDPSLLALWMIWTG----VPTLTFCTKVSSQQIPVLVQQDVLQLLMKSGVYPPTCSPDNGPSVGIHCVGGRRGIVRRALQREDSVRALLDSGVSVNRTDPSDGRTL-LSTAAHAGLVDIVALLLCRGADPSLSDHQGQTALTLVARQGHIGVLQILLEWAQDQGTDTPAVQALLEHADSDGWTALRSAAWGGHNEAVKMLLEAGADVDGCDPDGRTALRAAAWGGHEEVLMTLLEHGAKVDCMDHEGRTPLIAAAYMGHKEAVEIILDAGAEVDLADGDGRTALSVAALCVPSATGGRGHREVVSLLLERSAEPEHKDKDGMTPLLLASYEGHEEVVELLLEAGADVDEGTGAYPSAVTPLLAAAAMGHAGTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSVEVVKALLDRGLDENHKDDLGWTPLHAAACDGHKSVCAVLTERGSMARVGELDVEGRTALILAAQEGHCSTVRLLLDR-KSPIDHRSYDGHSALSAASLQGHTEVVELLLRRGADTDVRDAEGRPLLYLLILEGRLDIATLLIEKGGV----PLESRDAEGRTALHVAAWQGDLDGIGLLLGHGADPNALDQEGRPPLHSVAWRGLTSAGRLFLRARDINVDQACKKQGATALSIAAQEGHVEIVAMLLEKGADPDHVDHYGRTPVKVAGKRGLFNIIRLLESYGA 1125          

HSP 2 Score: 151.369 bits (381), Expect = 1.772e-36
Identity = 129/450 (28.67%), Postives = 213/450 (47.33%), Query Frame = 2
Query: 2084 VKLLLNNGAYIDHQD-NDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLE----NNADIDIVDR----------SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC--------QEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNI-----NNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFG--INIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            V+ LL++G  ++  D +DGR+ +  AA     ++  +DI+ LLL    + +L D++G   L + A +G   + +ILLE       D   V            + L +AA+ G+ E V++LL  GA +   D  G + L  +A  G+ ++L  LL  G      D+ G TPL  A+  G  E V ++L +GA+ +  D  GR  L ++           G+ ++   L++   E    K  DG T L +A      E VE LL  GAD  + +  G  P  ++ L +        + H+ T N        ++ +D +GRTA+ +A+ KG  E++K+L+D G   + KD+ G T LH  A  G   +C +L + G    + E   +G TAL +A+QEGH     LL++  + + H      +    A    H ++  LL + GA  D ++ 
Sbjct:  528 VRALLDSGVSVNRTDPSDGRTLLSTAA-----HAGLVDIVALLLCRGADPSLSDHQGQTALTLVARQGHIGVLQILLEWAQDQGTDTPAVQALLEHADSDGWTALRSAAWGGHNEAVKMLLEAGADVDGCDPDGRTALRAAAWGGHEEVLMTLLEHGAKVDCMDHEGRTPLIAAAYMGHKEAVEIILDAGAEVDLADGDGRTALSVAALCVPSATGGRGHREVVSLLLERSAEP-EHKDKDGMTPLLLASYEGHEEVVELLLEAGAD--VDEGTGAYPSAVTPLLAA-----AAMGHAGTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSVEVVKALLDRGLDENHKDDLGWTPLHAAACDGHKSVCAVLTERGSMARVGELDVEGRTALILAAQEGHCSTVRLLLDRKSPIDHRSYDGHSALSAASLQGHTEVVELLLRRGADTDVRDA 964          

HSP 3 Score: 135.576 bits (340), Expect = 1.236e-31
Identity = 102/335 (30.45%), Postives = 158/335 (47.16%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGS--NINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNA----DIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCID-----------------------------------KSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKP 2617
            +  T L+ AA  G     N +L  GA    ID +G +AL LA   G +EVVK LL + +D +   + G T L  AA  G   +  +L   GS   + + D EGRT L+ A  +     V+LLL+  + IDH+  DG SA+  A+L       + ++++LLLR   + ++ D EG  +L +   EG   +  +L+E         D   R+ L  AA+ G+ + + LLL  GA  + +D                                   K G + LSI+A +G+ +I++ LL +G D  H D++G TP+ +A   G +  + LL   GAKP
Sbjct:  798 SAVTPLLAAAAMGHAGTVNRMLFWGAAVDGIDGEGRTALCLAAAKGSVEVVKALLDRGLDENHKDDLGWTPLHAAACDGHKSVCAVLTERGSMARVGELDVEGRTALILAAQEGHCSTVRLLLDRKSPIDHRSYDGHSALSAASL-----QGHTEVVELLLRRGADTDVRDAEGRPLLYLLILEGRLDIATLLIEKGGVPLESRDAEGRTALHVAAWQGDLDGIGLLLGHGADPNALDQEGRPPLHSVAWRGLTSAGRLFLRARDINVDQACKKQGATALSIAAQEGHVEIVAMLLEKGADPDHVDHYGRTPVKVAGKRGLFNIIRLLESYGAKP 1127          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 230.72 bits (587), Expect = 5.293e-61
Identity = 164/545 (30.09%), Postives = 267/545 (48.99%), Query Frame = 2
Query: 1706 NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            +G+DIN  N+NG  +L +A++ G   +   L+  GA      K G +AL +A   G  +VVK L++   + +    +G T L  AA     D+V  LL +GS+ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +V+LLL  GA I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL      +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +    L+ H  + N  N+      G TA+H+A+  G  E++K L+  GA +D K  + +T LH+ +  G  +I Q L+  G   D T + G T L +A++EGH ++ ++L++ GA++        TP  VA K   I++ NLL +  A  D
Sbjct:   10 SGVDINICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVR-----GETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPD 539          

HSP 2 Score: 212.231 bits (539), Expect = 3.500e-55
Identity = 169/669 (25.26%), Postives = 307/669 (45.89%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID--VWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            IN+ N N LNA     K+      T ++ + +T   + +  N    +L  +      + + +L+ NG ++N  ++NG T L +AAQ     +   LL +G++Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++ +     TPL  A+ + +  MVKLLL  GA ID +  DG + +   A                            L + +   +L+ ++LLL +   ++ + N+ +  L +AA+ G   + K++++  A+ +   +   +PL  A      +V+ LLL  GA+I  + +SG + + ++A  G+  I+++L+  G      +  G T LH+A+  GQ E V  L+++GA  +      + PL +S + G  +I ++L+     P+S     Y   T L +A      +    LL +GA   I    G TPLH++    + +   +LLQ     + +        G T +H+A+   + ++   L+D GA        G T LH+ A +   +I   L+++G + +    QG + L +ASQEG++++  LL+   A +   +    TP  +A + + + +  +L  HGA VD
Sbjct:   14 INICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNT---ALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGY---TPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASG-----KSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVD 671          

HSP 3 Score: 201.445 bits (511), Expect = 8.192e-52
Identity = 161/557 (28.90%), Postives = 260/557 (46.68%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            S R NS N +  L+  G  I+   ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL  ++  D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA I      G + I VAA   + N     I+  L+ +  + N  +  G   L +AA  G   + K L++N A +D     D++PL  ++  G  E+++ LL  GA       SG++ L ++A +G+  + S LL +G         G TPLH+A+  G+ E  NLLL+  A P+     G  PL ++                        YD     K+A+L         LL +GA       NG TPLHI+  ++Q +    LL++       + N +   G + +H+AS +G+ +++  L+   A I+L +  G T LHL A +    + ++L++ G  +D     G T L +A   G++++ + L+   A V       +T    A +G H  I NLL +HGA
Sbjct:  223 SKRGNS-NMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHEN-----IVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAH----------------------YDNQ---KVALL---------LLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGA-----DTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKV-----NAKTKVTAAQQG-HTHIINLLLQHGA 728          

HSP 4 Score: 159.073 bits (401), Expect = 1.047e-38
Identity = 128/482 (26.56%), Postives = 217/482 (45.02%), Query Frame = 2
Query: 1898 LSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            L   +D +    +G  AL  A+  G  ++V  L+  G+ ++    +G T L  A+      +VK L+ NGA ++ Q  +G + +++AA        +LD+++ LL    + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG     +     TPLH+AS  G    V LLL+ GA+                                  ++ K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LL H +      ++ + +D  TA+H+A+  G  ++ K ++D  A  + K   G T LH+   +   ++ +LL+  G +I      G T + +A+  GH  I   L+N GA+    +    T   +A +    ++   L ++GA VD K+
Sbjct:    8 LKSGVDINICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAA-----QENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGAR----------------------------------IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVP-----VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKS 443          

HSP 5 Score: 156.377 bits (394), Expect = 5.798e-38
Identity = 129/465 (27.74%), Postives = 215/465 (46.24%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +V  L++    P+ T   G TAL  AA  G+T++V  L+ +G+ ++ +  + +TPL  ++      +++ LL +GA  D     G + + +AA        + D+  +LL    ++ +   +G   L +AA  G   +  +LL+  A  D   +S   PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +   +I + LL  G D       G +PLHLAS EG  + V LL+   A  N  +  G  PL L+ QE  + +AE L++    +V+ ++  G T L +A      + V FLL   A       N +T +  +  Q       +LLQH    N   +N     G TA+ IA   G   ++ +L
Sbjct:  309 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAA-----REGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHG-ATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKV-----NAKTKV-TAAQQGHTHIINLLLQHGAAPNELTVN-----GNTALSIARRLGYISVVDTL 756          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 226.483 bits (576), Expect = 1.278e-59
Identity = 161/544 (29.60%), Postives = 264/544 (48.53%), Query Frame = 2
Query: 1709 GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            G+DIN  N+NG  +L +A++ G   +   LL  GA+     K G +AL +A   G  +VV+ L++   + +    +G T L  AA     ++V  LL  G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D + R   +PL  AA  GN  +V+LLL  GA I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +  + L  H  + N  N+      G TA+H+A+  G  ++++ L+  GA++D+K  + +T LH+ +  G  +I QLL+  G       + G T L +A +EGH E  +LL+  GA++        TP  VA K   I+   LL +  A  D
Sbjct:   66 GVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVR-----GETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPD 594          

HSP 2 Score: 202.986 bits (515), Expect = 2.441e-52
Identity = 158/556 (28.42%), Postives = 258/556 (46.40%), Query Frame = 2
Query: 1652 LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLE 3310
            L  + ++ + N +  L+  G  I+   ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA +      G + I VAA   + N     I+K L  +  + N  +  G   L +AA  G   + + LL+N A +DI    D++ L  A+  G  E+V+LLL  GA       SG++ L ++  +G+ +  S LL  G         G TPLH+A+  GQ E   LLL+  A P+     G  PL ++      ++A  L+D    S +  + +G T L IA      E    LL  GA+       G +PLH++  +   D   +LL    T N N +N  + +G T +H+A+ +    + + L++ GA +D     G T LH+    G  ++   L++    I+     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L 
Sbjct:  274 LHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHEN-----IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHG-ASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLL----TKNAN-VNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTLR 818          

HSP 3 Score: 202.216 bits (513), Expect = 4.421e-52
Identity = 169/632 (26.74%), Postives = 288/632 (45.57%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKID-------------PDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            IN+ N N LNA     K+        +L + ++   + +  N    +L  +      + + +L+ NG ++N  ++NG T L +AAQ     +   LL  GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D              D     G T L  AA  G  ++  LLL+ G+ ++       TPL  A  + +  MVKLLL+ GA ID +  DG + +   A      S +  ++++LL     I      G++ L +A  +G  L C ++LL+++  +D V    L A   AA  G+++V ++++   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V  L   GA PN  +  G   L ++ + G   +   L+    + V+ K+ D  T+L IA      E V+ LL +GA A+    +G TPLH++V +   +   +LL+H  +     ++     G T +H+A+  G  E  K L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  LEI   L+ FGA    +     +P  +A +   + + +LL
Sbjct:   69 INICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNT---ALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGA-----RSGHEQVVEMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAK-VDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGAS-----LSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLL 685          

HSP 4 Score: 167.933 bits (424), Expect = 1.613e-41
Identity = 134/465 (28.82%), Postives = 215/465 (46.24%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +VK L      P+ T   G TAL  AA  G+ D+V  LL +G+ ++ +  + +T L  A+      +V+LLL  GA+       G + + +A         + +   LLL +  +++    +G   L +AA  G     K+LL+  A  D   +   +PL  AA   N  V  LLL  GA+ H   K+G++ L I+A +   +I + LL  G +       G +PLHLA+ EG  + V+LLL   A  N  + +G  PL L+ QE  + +AE L++   E V+  +  G T L +A      + V FLL   A    +  +G TPLH +  Q       +LLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  364 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAV-----REGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAE-VDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLN-----GNTALSIARRLGYISVVDTL 817          

HSP 5 Score: 142.124 bits (357), Expect = 1.608e-33
Identity = 129/498 (25.90%), Postives = 211/498 (42.37%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A   G +E     L   +D +    +G  AL  A+  G  ++V  LL  G++++    +G T L  A+      +V+ L+ NGA ++ Q  +G + +++AA        +L++++ LL +  + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL          KSGF+ L I+A  GN  + + LL RG  +D M R++   TPLH+A+  G    V LLL  GAK                                  ++ K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LLQH +                                       +D   N+  T LH+ A  G Y++ ++++D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H  I   L  HGA  +  NV
Sbjct:   50 AARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDI--TPLHVAAKRGNGNMVKLLLDRGAK----------------------------------IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNV 466          

HSP 6 Score: 137.887 bits (346), Expect = 3.229e-32
Identity = 104/373 (27.88%), Postives = 175/373 (46.92%), Query Frame = 2
Query: 1589 ILNITSTYSNSIEATNVY-SFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            I+   + +  S   TNV    +L  + R    + +  L+ NG  ++   ++  T+L +A++ G   I  LLL  GA        G++ L LA+  G+ E   LLL            G T L  AA  G+ +   LLL   +  +     G TPL  A +  +  +  LLL++GA       +G + + +AA         L+I   LL +    N +  +G++ L +AA EGS  L  +LL  NA++++ ++   +PL  AA      V  +LL  GA +    K+G++ L ++   GN +++S LL        +   G TPLH A+ +G    +N+LL+  A  NE+  +G   L ++ + GYI + + L
Sbjct:  450 IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAA-----KKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 817          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1])

HSP 1 Score: 219.55 bits (558), Expect = 1.989e-57
Identity = 161/552 (29.17%), Postives = 265/552 (48.01%), Query Frame = 2
Query: 1709 GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLID--------NEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            G+DIN  N+NG  +L +A++ G   +   LL  GA+     K G +AL +A   G  +VV+ L++   + +    +G T L  AA     ++V  LL  G++ +    +G TPL  A  +    +V LLL         +ND +  + + AL + +         LLL+ + N ++          + G   L IAA+ G+  +  +LL   A +D + R   +PL  AA  GN  +V+LLL  GA I    K G + L   A  G+ Q++  LL RG   + +   G +PLH+A+      CV LLL+     +++ N     L ++   G+ K+A+ ++D    + N K+ +G T L IA      + +E LL  GA       +G TP+H++      +  + L  H  + N  N+      G TA+H+A+  G  ++++ L+  GA++D+K  + +T LH+ +  G  +I QLL+  G       + G T L +A +EGH E  +LL+  GA++        TP  VA K   I+   LL +  A  D
Sbjct:   30 GVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKK-ANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVR-----GETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPD 566          

HSP 2 Score: 202.986 bits (515), Expect = 2.463e-52
Identity = 158/556 (28.42%), Postives = 258/556 (46.40%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
             L  + ++ + N +  L+  G  I+   ++G T L   A++G   +  +LL  GA  L   K+G S L +A    ++  V+LLL   +  D   ND  TAL  AA  G   +  +++   +N N +   G TPL  A  K    +++LLL +GA +      G + I VAA   + N     I+K L  +  + N  +  G   L +AA  G   + + LL+N A +DI    D++ L  A+  G  E+V+LLL  GA       SG++ L ++  +G+ +  S LL  G         G TPLH+A+  GQ E   LLL+  A P+     G  PL ++      ++A  L+D    S +  + +G T L IA      E    LL  GA+       G +PLH++  +   D   +LL    T N N +N  + +G T +H+A+ +    + + L++ GA +D     G T LH+    G  ++   L++    I+     G T L  A+Q+GH  I N+L+   A+   +     T   +A +  +I + + L
Sbjct:  245 PLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHEN-----IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHG-ASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLL----TKNAN-VNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 789          

HSP 3 Score: 196.823 bits (499), Expect = 2.025e-50
Identity = 170/640 (26.56%), Postives = 289/640 (45.16%), Query Frame = 2
Query: 1526 INLVNINKLNA-----KKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLL----------------SQKIDPDFTG----ND-----------------GRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            IN+ N N LNA     K+        +L + ++   + +  N    +L  +      + + +L+ NG ++N  ++NG T L +AAQ     +   LL  GA+Q +  +DG++ L +A+  G+ +VV LLL                ++K D         ND                 G T L  AA  G  ++  LLL+ G+ ++       TPL  A  + +  MVKLLL+ GA ID +  DG + +   A      S +  ++++LL     I      G++ L +A  +G  L C ++LL+++  +D V    L A   AA  G+++V ++++   A  +    +GF+ L I+  +   +++  LL  G         G TP+H+A+  G    V  L   GA PN  +  G   L ++ + G   +   L+    + V+ K+ D  T+L IA      E V+ LL +GA A+    +G TPLH++V +   +   +LL+H  +     ++     G T +H+A+  G  E  K L+   A  D     G T LH+ A     ++  LL+D G +       G T L IA+++  LEI   L+ FGA    +     +P  +A +   + + +LL
Sbjct:   33 INICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNT---ALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGA-----RSGHEQVVEMLLDRGAPILSKTKNGLSPLHMAT-QGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAK-VDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGAS-----LSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLL 657          

HSP 4 Score: 168.318 bits (425), Expect = 1.586e-41
Identity = 134/465 (28.82%), Postives = 215/465 (46.24%), Query Frame = 2
Query: 1625 EATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            + TN Y  +L  +     Y     ++    + N    NG T L +A +     +  LLL  GA+   + + G + + +A + G+  +VK L      P+ T   G TAL  AA  G+ D+V  LL +G+ ++ +  + +T L  A+      +V+LLL  GA+       G + + +A         + +   LLL +  +++    +G   L +AA  G     K+LL+  A  D   +   +PL  AA   N  V  LLL  GA+ H   K+G++ L I+A +   +I + LL  G +       G +PLHLA+ EG  + V+LLL   A  N  + +G  PL L+ QE  + +AE L++   E V+  +  G T L +A      + V FLL   A    +  +G TPLH +  Q       +LLQH  + N   +N     G TA+ IA   G   ++ +L
Sbjct:  336 DVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAV-----REGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAE-VDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLN-----GNTALSIARRLGYISVVDTL 789          

HSP 5 Score: 138.272 bits (347), Expect = 2.532e-32
Identity = 104/373 (27.88%), Postives = 175/373 (46.92%), Query Frame = 2
Query: 1589 ILNITSTYSNSIEATNVY-SFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            I+   + +  S   TNV    +L  + R    + +  L+ NG  ++   ++  T+L +A++ G   I  LLL  GA        G++ L LA+  G+ E   LLL            G T L  AA  G+ +   LLL   +  +     G TPL  A +  +  +  LLL++GA       +G + + +AA         L+I   LL +    N +  +G++ L +AA EGS  L  +LL  NA++++ ++   +PL  AA      V  +LL  GA +    K+G++ L ++   GN +++S LL        +   G TPLH A+ +G    +N+LL+  A  NE+  +G   L ++ + GYI + + L
Sbjct:  422 IVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAA-----KKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 789          

HSP 6 Score: 135.961 bits (341), Expect = 1.303e-31
Identity = 129/506 (25.49%), Postives = 213/506 (42.09%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP-LMAAAFSGNFEVVRLLLF------WGAAIHCIDK--SGFSVLSISASQGNSQILSELLARG--LDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            A   G +E     L   +D +    +G  AL  A+  G  ++V  LL  G++++    +G T L  A+      +V+ L+ NGA ++ Q  +G + +++AA        +L++++ LL +  + ++   +G   L +A  +G   +  +LLEN+    +  R P L  AA   + +   LLL         + +H      SGF+ L I+A  GN  + + LL RG  +D M R++   TPLH+A+  G    V LLL  GAK                                  ++ K+ DG T L     +   + VE LL RGA    +  NG +PLH++      +C Q+LLQH +                                       +D   N+  T LH+ A  G Y++ ++++D   N +     G T L IA ++  +++  LL+  GA++  +     TP  VA    H  I   L  HGA  +  NV
Sbjct:   14 AARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAA-----QENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKV--RLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDI--TPLHVAAKRGNGNMVKLLLDRGAK----------------------------------IDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVP--------------------------------------VDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNV 438          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 243.047 bits (619), Expect = 1.822e-67
Identity = 174/561 (31.02%), Postives = 278/561 (49.55%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSKY---------------LDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +  TS + AA+NG        L  G +    +++G +AL LA   G+++VV  LL ++ + D     G TAL  AA  G+TDIV +L+  G+NIN Q   G TPL  A  +    +VK LL+NGA       DG + + VA          L L S++K                 +   LLL++  N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  AA  GN  +V+LLL  GA I    + G + L  +A  G+ +++  LL R    + +   G +PLH+A+      CV LLL   A  +++      PL ++   G+ ++A+ L+D   E  N ++ +G T L IA      + +E LL  GA       +G TP+H++      +  Q+LLQ+  + N  N+      G T +H+AS  G  E++K+L+  GAR+ +K  E +T LH+ +  G  +I QLL+  G + +   + G+T L  AS+EGH ++ +LL+  GA++  +     TP  +A K   +++  LL +  A  D K
Sbjct:   10 DAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEP-NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVR-----GETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPK 564          

HSP 2 Score: 196.052 bits (497), Expect = 6.845e-52
Identity = 166/568 (29.23%), Postives = 269/568 (47.36%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSR--GADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +L  + R++  N  + L+ +  + +  +++G T L +AA  G  ++  LLL+ GA      ++G + L +A   G   +VKLLL +    D    DG T L  AA  G   ++ +LL   + I  +   G +PL  AT       V+LLL   A +D    D  + + VAA C      +  + KLLL      N     G   L IA  +    + ++LL++ A I     S   P+  A+F G+  VV+LLL  GA+ +  +  G + L +++  G  +++  LL  G     +     TPLH+AS  G+ E V LLL+ G+ PN     G  PL  + +EG+  +A  L++   + ++  +  G T L IA      E    LL +   AD  +   NG TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   E+ K L+++GA  ++   +G   LHL A +G   +  LL+    +I      G TA+ +A+QE  + +  +L    A+V        TP  VA    +IK+ N L ++ ALV+ K
Sbjct:  175 ALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHC-----GHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGAD-ISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVA---THYDHQKVALL--LLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAK 731          

HSP 3 Score: 181.415 bits (459), Expect = 3.360e-47
Identity = 156/562 (27.76%), Postives = 252/562 (44.84%), Query Frame = 2
Query: 1652 LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLK--SGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            L  + ++ + N +  L+  G  I+    +G T L  AA++G   +  +LL   A  L   K+G S L +A    ++  V+LLL ++   D    D  T L  AA  G   +  LLL  G   N +   G TPL  A  K    +++LLL +GA I      G + I VA+        +L++++LLL+   + N  +  G   L +A+  G   + K LL++ A + +    D++PL  A+  G  E+V+LLL  G+  +    SG + L  ++ +G+  + S LL  G D       G TPLH+AS  G+ E   LLL+  + A P     +G  PL ++                        YD     K+A+L         LL +GA       NG TPLHI+  ++Q +  +VLL+          N L   G   +H+A+ +G  +++  L+   A I L    G T +HL A +    + ++L     +++ T     T L +AS  G++++ N L+   A V        TP   A +  H  + N L +H A
Sbjct:  242 LHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFM-----GHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATH----------------------YDHQ---KVALL---------LLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA-----ETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEA 759          

HSP 4 Score: 137.502 bits (345), Expect = 2.958e-33
Identity = 126/453 (27.81%), Postives = 196/453 (43.27%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLEN--NADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            ++N    +  L+ +G  I+   E+G T + VA+  G  ++  LLL +GA+    +  G + L +A   G +EVVK LL           + +T L  A+ +G+ +IV LLL  GS+ N   + G TPL  A+ +    +  LLL NGA                                      +I+ +  +G   L IA+  G   +  +LLE   +AD  +  +   +PL  A    + +V  LLL  GA+ H   K+G++ L I+A +   ++   LL  G +       G  PLHLA+ EG  + V LLL   A  +     G   + L+ QE  + +AE ++      VN  +    T L +A      + V FLL   A    +  NG TPLH +  Q        LLQH  +      N L  +G TA+ IA   G   ++ +L
Sbjct:  379 KKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGA--------------------------------------DISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAE-ILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEAS-----PNELTANGNTALAIARRLGYISVVDTL 787          

HSP 5 Score: 127.102 bits (318), Expect = 6.307e-30
Identity = 104/398 (26.13%), Postives = 184/398 (46.23%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L S   ++D++  LL+    I+     G   L IAA  G   + ++L+E  A+I+   +   +PL  AA   + E+V+ LL  GA      + GF+ L+++  QG+ Q++  LL    +   +       LH+A+ +       LLL+     +     G  PL ++   G + +A  L+++   +V+F + +G T L +A        V+ LL RGA    +  +G TPLH +         ++LL+         I     +G + +H+A+       ++ L+   A +D    +  T LH+ A  G Y++ +LL+D G   +     G T L IA ++  +++  LL+  GA++        TP  VA    H+ +  LL ++GA  +  NV
Sbjct:   47 ALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLL----ESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLG-AAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAP-----ILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNV 434          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 237.269 bits (604), Expect = 4.124e-65
Identity = 175/569 (30.76%), Postives = 278/569 (48.86%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA---------ALCLNSNSKY---------------LDIIKLLLRYNCN--------INLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +  TS + AA+NG        L  G +    +++G +AL LA   G+++VV  LL ++ + D     G TAL  AA  G+TDIV +L+  G+NIN Q   G TPL  A  +    +VK LL+NGA       DG + + VA          L L S++K                 +   LLL++  N        +N     G   L IAA+ G+  +  +LL   A +D   R   +PL  AA  GN  +V+LLL  GA I    + G + L  +A  G+ +++  LL R    + +   G +PLH+A+      CV LLL   A  +++      PL ++   G+ ++A+ L+D   E  N ++ +G T L IA      + +E LL  GA       +G TP+H++      +  Q+LLQ+  + N  N+      G T +H+AS  G  E++K+L+  GAR+ +K  E +T LH+ +  G  +I QLL+  G + +   + G+T L  AS+EGH ++ +LL+  GA++  +     TP  +A K   +++  LL +  A  D K
Sbjct:    9 DAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEP-NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVR-----GETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPK 571          

HSP 2 Score: 197.978 bits (502), Expect = 2.848e-52
Identity = 156/566 (27.56%), Postives = 264/566 (46.64%), Query Frame = 2
Query: 1718 INCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLN-------------SNSKYLD---------------IIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNAD---IDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +N   E+G T L +AA  G  ++  LLL+ GA      ++G + L +A   G   +VKLLL +    D    DG T L  AA  G   ++ +LL   + I  +   G +PL  AT       V+LLL   A +D    D  + + VAA C +              N++ L+               +++LLL++  +I+     G+  + +A++ G   + ++LL+N A     ++   +PL  A+ +G  EVV+ LL  GA +    +   + L I++  G ++I+  LL RG D       G TPL+ AS EG  +  +LLL++GA  + +   G  PL ++ + G +++A  L++ +  S + K  +G T L +A      +    LL +GA       NG TPLHI+  ++Q +  +VLL+          N L   G   +H+A+ +G  +++  L+   A I L    G T +HL A +    + ++L     +++ T     T L +AS  G++++ N L+   A V        TP   A +  H  + N L +H A
Sbjct:  205 LNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKY-ASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGA-----ETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEA 764          

HSP 3 Score: 193.356 bits (490), Expect = 9.320e-51
Identity = 166/575 (28.87%), Postives = 265/575 (46.09%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMA---ATYKCDYVMVKLLLNNGAYID--------HQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL---ARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +L  + ++   + +++L+    +I+   + G T+L +AA  G T I  +L+  GAN     ++G++ L +A    ++E+VK LL    +      DG T L  A   G   +V LLL S       D++G+  L A   A  K D     LLL +    D             G + + +AA   N N     +  LLL     ++     G+  L +AA  G+  + K+LL+  A ID   R   +PL  AA SG+ +V+ +LL   A I    K+G S L + A+QG      +LL      +D++  D    TPLH+A+  G Y    LLL  G +PN    +G  PL ++C++  IK+ E L+     S++  +  G T + +A        V+ LL  GA +   +  G TPLH++    Q +  + LLQ         +     + +T +HIAS  G  EI++ L+  G+  +     G T L+  + +G   +  LL++ G +I     +G T L IAS+ G LE+  LL+   A+         TP  VA   +H K+  LL   GA
Sbjct:   46 ALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLES-------DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMN-----VATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHM-ATQGEHLACVQLLLGRQAPVDDVTLDYL--TPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLK-HGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGA-----RVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGA 599          

HSP 4 Score: 139.043 bits (349), Expect = 1.301e-33
Identity = 129/450 (28.67%), Postives = 196/450 (43.56%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKY--LDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            ++N    +  L+ +G  I+   E+G T + VA+  G  ++  LLL +GA+    +  G + L +A   G +EVVK LL           + +T L  A+ +G+ +IV LLL  GS+ N   + G TPL  A+ +    +  LLL NGA I      G + + +A       SKY  L++  LLL    +    D +G N L                           +PL  A    + +V  LLL  GA+ H   K+G++ L I+A +   ++   LL  G +       G  PLHLA+ EG  + V LLL   A  +     G   + L+ QE  + +AE ++      VN  +    T L +A      + V FLL   A    +  NG TPLH +  Q        LLQH  +      N L  +G TA+ IA   G   ++ +L
Sbjct:  386 KKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIA-------SKYGRLEVATLLLEKYAS---ADPKGKNGL---------------------------TPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAE-ILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEAS-----PNELTANGNTALAIARRLGYISVVDTL 792          

HSP 5 Score: 124.02 bits (310), Expect = 6.067e-29
Identity = 109/418 (26.08%), Postives = 190/418 (45.45%), Query Frame = 2
Query: 2210 LRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDI---VDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAK-----P--------------------------------NEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            L+   +IN  +  G+N L +A+ EG   +   LL+  A+ID    V  + L  AA +G  ++VR+L+ +GA I+   ++GF+ L ++A + + +I+  LL  G ++      G TPL +A  +G  + V LLL+S  K     P                                N+    G  PL ++   G + +A  L+++   +V+F + +G T L +A        V+ LL RGA    +  +G TPLH +         ++LL+         I     +G + +H+A+       ++ L+   A +D    +  T LH+ A  G Y++ +LL+D G   +     G T L IA ++  +++  LL+  GA++        TP  VA    H+ +  LL ++GA  +  NV
Sbjct:   30 LKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLG-AAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAP-----ILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNV 441          

HSP 6 Score: 95.5153 bits (236), Expect = 3.749e-20
Identity = 110/462 (23.81%), Postives = 183/462 (39.61%), Query Frame = 2
Query: 1379 IFKFKGYHFAVWLLY----SDCDIEVKGILP---CEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTY-----ILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESV 2719
            +  F G+   V LL     S     V+G  P     + G L      ++VK L +S   V +     +    I S       +  +    S+    T   S  L+ + R+   +  S L+ NG DI+ V + G T L +A++ G   +  LLL   A+     K+G + L +A    + +V  LLL +   P  T  +G T L  AA   + ++  +LL  G+  N    +G  PL  A  +    MV LLL            GR A                          +I+L    G+  + +AA E    + +IL ++ AD++   +   +PL  A+  GN ++V  LL   A ++   ++G++ L  +A QG++ +                                 VN LL+  A PNE+  +G   L ++ + GYI + + L  +  E V
Sbjct:  416 VASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQL------EVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLL------------GRHA--------------------------SISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHV---------------------------------VNALLQHEASPNELTANGNTALAIARRLGYISVVDTLSILTEEPV 800          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 194.126 bits (492), Expect = 1.989e-50
Identity = 153/534 (28.65%), Postives = 243/534 (45.51%), Query Frame = 2
Query: 1730 NENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
             ++G T L  AA++G   +  +LL  GA  L   K+G S L +A    ++E V++L+  +   D    D  TAL  AA  G      +LL  G+N N +   G TPL  A  K    +++LLL  GA I+     G + + V+A   N     L+I+ +LL+   + N  +  G   L +AA  G   + + LL N A +D   R   +PL  AA  G  E+V+LLL   A+      +G++ L ++A +G+  + + LL  G         G TPLH+AS  G+ E  +LLL+  A P+    +G  PL ++                        YD     K+A+L         LL +GA       NG TPLHI+  + Q +    LL++         N +   G   +H+ + +G  ++   L+   A ++     G + LHL A +    + ++L+  G +++ T   G T L +A   G++++ N L+  GA+V        TP   A +  H  I NLL K GA
Sbjct:    3 RQDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGN-----LNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASH----------------------YDNQ---KVALL---------LLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGA-----ETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGA 492          

HSP 2 Score: 180.259 bits (456), Expect = 4.479e-46
Identity = 142/473 (30.02%), Postives = 225/473 (47.57%), Query Frame = 2
Query: 1931 NDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
             DG T L  AA  G   +V +LL  G+ I  +   G +PL  AT       V++L+   A +D    D  +A+ VAA C      +    K+LL    N N+    G   L IA  +    + ++LL+  A I+ V  S   P+  +AF GN  +V +LL  GA+ +  +  G + L ++A  G ++++  LL  G     +     TPLH+A+  G+ E V LLL+  A P+   ++G  PL ++ +EG+  +   L++    S+   +  G T L +A      E    LL R A       NG TPLH++   S YD  +V L   L     + +    +G T +HIA+ K   EI  +L++ GA  +    +G   LHLGA +G   +  LL+    +++     G + L +A+QE  + +  +L+  GA+V        TP  VA    +IK+ N L +HGA V+ K 
Sbjct:    4 QDGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHC-----GHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLE-HGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVA---SHYDNQKVALL--LLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKT 465          

HSP 3 Score: 177.948 bits (450), Expect = 2.233e-45
Identity = 137/519 (26.40%), Postives = 240/519 (46.24%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLL 3208
            + R      +  L+  G  I    +NG + L +A Q        +L+   A    I  D  +AL +A   G+    K+LL +  +P+    +G T L  A    R  ++ LLL  G++I      G TP+  + +  +  +V +LL  GA  +  +  G +A+ +AA      +   ++++ LLR    ++    E    L IAA  G   + ++LL++ A  D       +PL  AA  G+ +V  +LL  GA++    K GF+ L +++  G  ++ S LL R          G TPLH+AS     +   LLL  GA P+    +G  PL ++ ++  ++IA  L++   E+ N  +  G   L +       +    LL+R AD      +G +PLH++  + + +  ++L++H       ++N     G T + +A   G+ +++  L+  GA ++ K   G T LH  A QG   I  LL+  G   ++    G TAL+IA + G++ + + L
Sbjct:   13 AARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAA-----RAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAET-NAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGA-----HVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTL 520          

HSP 4 Score: 157.918 bits (398), Expect = 3.168e-39
Identity = 128/479 (26.72%), Postives = 208/479 (43.42%), Query Frame = 2
Query: 1505 VKILFESINLVNINKLNAKKSFTIF----STYILNITSTYSNSIEATNVYSFS-LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDN 2833
             K+L +     N+  LN      I        ++ +   Y  SIEA      + +  S    + N +  L+  G   N  N  G T+L +AA+ G T +   LL +GA      ++  + L +A   G +E+V+LLL     PD   ++G T L  AA  G  D+  +LL  G+++     +G TPL  A+      +  LLL   A  D    +G + + VA+                            L + +    L+I   LL Y    N I  +G+  L + A EG   +  +LL   AD++   +S   PL  AA      V  +L+  GA ++   K G++ L ++   GN ++++ LL  G D   +   G TPLH A+ +G    VNLLLK GA+PN++  +G   L ++ + GYI + + L  V  E++   +    +S ++ V   + E +E     G D    D 
Sbjct:   88 AKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTLKVVTEETITSTTVSNPSS-RVNVPETMTEVLEVSDDEGEDTMTGDG 565          

HSP 5 Score: 58.151 bits (139), Expect = 1.314e-8
Identity = 46/172 (26.74%), Postives = 81/172 (47.09%), Query Frame = 2
Query: 2831 NNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKV-AYKGNHIKICNLLEKHGALVDRKNV 3343
             +G TPLH +         ++LL+         I     +G + +H+A+     E ++ L+   A +D    +  T LH+ A  G Y+  ++L+D G N +     G T L IA ++  + +  LL+ +GA++  +     TP  V A+ GN + I  +L + GA  +  NV
Sbjct:    4 QDGLTPLHCAARSGHEQVVEILLEQGAP-----ILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGN-LNIVLILLQGGASPNATNV 169          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008129.1 (pep scaffold:Pmarinus_7.0:GL476358:593185:649018:1 gene:ENSPMAG00000007339.1 transcript:ENSPMAT00000008129.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 178.333 bits (451), Expect = 1.610e-45
Identity = 212/840 (25.24%), Postives = 345/840 (41.07%), Query Frame = 2
Query:  248 KLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKY---FGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPP----PNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLN-KSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIK--------SNGYIIYLEIVLDGILDGYLSIQV--IKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYY--LKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLL--DVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATN------VYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDG---------RTALRTAAWIGRTDIVLLLLSSGSNINKQDSE-----------GRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAK 2614
            +L + +++ H+C A +  +  + +F+      L +S     + E+  R  ++  + +L   ++    P  AF+ GIL PL +       P EN  ILID +                 N+  FHK   G   I+  L+     FP WL LV T R + + IV  F  F    LD    +  +  D+Q Y+ +R+     ++Q ++  T  L  +   +        S G  +YL + LD I  G+L ++    K +P  L  LYL      F     F  V  + N+ L+SL  L+D++ + A+    +   L +   +  +  +  FLV+  D    F H S  EWL     +  + K+ C    GH  LA   +   SKL   Q      H++  A I+K       V    S   ++   +           +S D +   L    +L N+   N K      S  ++   +  ++  EA        VYS+  ++ +        S L+  G D N  +E+G ++L  AA +G   I  +L   GA    +D+ G  AL  A   G+++++  LL  + +   TG            + AL   A  G  ++V  LL    + + +D             G T L AA  +    + +LLL  GA        G   +F A         +  +++LLL +  ++N+ D +G   L++++ EG       L    A +  VDR  L A   A   G   VVR LL  GAAI   DKSG + L ++A  G+  ++  L+  G    H DN G +PL  A        V  LL+ GA+
Sbjct:  332 RLAARVVSYHYCQADNAYTCLVPEFVHSTAALLCRSPQLPAYRELLMREPHALPVLSL---RSCVQDPAAAFRRGILEPLSTLRRERRIPEENFIILIDGL-----------------NEAEFHKPDYG-DTITSFLAKMLPRFPVWLKLVVTVRSKLQAIVEAFP-FHTVSLDGFGEREEISADLQAYVSSRVQSSCEIQQNVSLGTGRLDTIALGRLGGHLCSLSRGSFLYLRLTLDLIEWGHLVLKSSSYKVVPVSLAELYLLQCNMRFPTTSAFERVLPLLNVALASLHPLTDEQAFRAVAAGWMAPPLSWEDFQARMEMLSAFLVRRRDGTRMFCHPSFREWLTWRTGEGQSTKFLCDPRSGHALLAFLFSRQDSKLNRQQTIELGHHIL-KARIYKGLSKRLGV----SSSVLQALWVA----------FSTDGLSTAL---ASLRNLYTPNVK-----VSRLLIQAGADVNHRTEALGNAPILCVYSYLGYQDME-------SVLLEMGADPNLASESGMSALCYAASSGHLGIVRMLTRKGAKVDHVDRAGQCALVHAGLRGHLDIMIHLL--QAEWTLTGRQPGPVQRYQAVQQALTATASSGHVEVVSYLLDMLPDWDVEDLGRPHIDSFDTLWGETALTAAATQGRLEICRLLLEWGANASQASRQGVVPVFGAV-----RKGHWQVVELLLSHGVDVNVTDRQGRTPLMVSSSEGHLQTAAFLHSQGASVAAVDREGLSALSWACLKGRLPVVRFLLENGAAIEHADKSGRTPLDLAAFYGDENVIDCLIEHGASIDHTDNSGMSPLDRAIGCRNSSVVVALLRKGAR 1112          

HSP 2 Score: 108.612 bits (270), Expect = 4.745e-24
Identity = 99/356 (27.81%), Postives = 165/356 (46.35%), Query Frame = 2
Query: 2063 YKCDYVMVKLLLNNGAYIDHQDND-GRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLF--W---GAAIHCIDK--SGFSVLSISASQGNSQILSELL-------ARGLDEMHRDNF----GCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGL 3064
            Y  +  + +LL+  GA ++H+    G + I    LC+ S   Y D+  +LL    + NL    GM+ L  AA  G   + ++L    A +D VDR+    L+ A   G+ +++  LL   W   G     + +  +    L+ +AS G+ +++S LL          L   H D+F    G T L  A+ +G+ E   LLL+ GA  ++    G +P+  + ++G+ ++ E L+    + VN     G T L ++      +   FL S+GA     D  G + L  + L+ +    + LL+     N   I   D  GRT + +A++ GD  +I  LI+ GA ID  DN G + L
Sbjct:  746 YTPNVKVSRLLIQAGADVNHRTEALGNAPI----LCVYSYLGYQDMESVLLEMGADPNLASESGMSALCYAASSGHLGIVRMLTRKGAKVDHVDRAGQCALVHAGLRGHLDIMIHLLQAEWTLTGRQPGPVQRYQAVQQALTATASSGHVEVVSYLLDMLPDWDVEDLGRPHIDSFDTLWGETALTAAATQGRLEICRLLLEWGANASQASRQGVVPVFGAVRKGHWQVVELLLSHGVD-VNVTDRQGRTPLMVSSSEGHLQTAAFLHSQGASVAAVDREGLSALSWACLKGRLPVVRFLLE-----NGAAIEHADKSGRTPLDLAAFYGDENVIDCLIEHGASIDHTDNSGMSPL 1091          

HSP 3 Score: 95.1301 bits (235), Expect = 6.984e-20
Identity = 95/378 (25.13%), Postives = 153/378 (40.48%), Query Frame = 2
Query: 1961 AWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNIN------LIDNEGMNMLLIA-AYEGSKLLCKILLENNADIDIVD--------------RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARI 3031
            +++G  D+  +LL  G++ N     G + L  A       +V++L   GA +DH D  G+ A+  A L       +LDI+  LL+    +       +   + +   L A A  G   +   LL+   D D+ D               + L AAA  G  E+ RLLL WGA      + G   +  +  +G+ Q++  LL+ G+D    D  G TPL ++S EG  +    L   GA    +D  G   L  +C +G                                 RLP  V FLL  GA     D +GRTPL ++      +    L++H  +     I+  D+ G + +  A    +  ++ +L+  GAR+
Sbjct:  780 SYLGYQDMESVLLEMGADPNLASESGMSALCYAASSGHLGIVRMLTRKGAKVDHVDRAGQCALVHAGL-----RGHLDIMIHLLQAEWTLTGRQPGPVQRYQAVQQALTATASSGHVEVVSYLLDMLPDWDVEDLGRPHIDSFDTLWGETALTAAATQGRLEICRLLLEWGANASQASRQGVVPVFGAVRKGHWQVVELLLSHGVDVNVTDRQGRTPLMVSSSEGHLQTAAFLHSQGASVAAVDREGLSALSWACLKG---------------------------------RLP-VVRFLLENGAAIEHADKSGRTPLDLAAFYGDENVIDCLIEHGAS-----IDHTDNSGMSPLDRAIGCRNSSVVVALLRKGARL 1113          

HSP 4 Score: 83.1889 bits (204), Expect = 3.072e-16
Identity = 83/368 (22.55%), Postives = 154/368 (41.85%), Query Frame = 2
Query: 2294 LCKILLENNADI----DIVDRSPLMAA-AFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLK-----SGAKPNEIDNHGRI--PLMLSCQEGYIKIAEKLIDVWPE-------SVNFKSYD---GSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            + ++L++  AD+    + +  +P++   ++ G  ++  +LL  GA  +   +SG S L  +AS G+  I+  L  +G    H D  G   L  A + G  + +  LL+     +G +P  +  +  +   L  +   G++++   L+D+ P+         +  S+D   G T+L  A      E    LL  GA+A+     G  P+  +V                                       KG  ++++ L+  G  +++ D +GRT L + + +G  Q    L   G ++     +G +ALS A  +G L +   L+  GA + H D   RTP  +A       + + L +HGA +D
Sbjct:  752 VSRLLIQAGADVNHRTEALGNAPILCVYSYLGYQDMESVLLEMGADPNLASESGMSALCYAASSGHLGIVRMLTRKGAKVDHVDRAGQCALVHAGLRGHLDIMIHLLQAEWTLTGRQPGPVQRYQAVQQALTATASSGHVEVVSYLLDMLPDWDVEDLGRPHIDSFDTLWGETALTAAATQGRLEICRLLLEWGANASQASRQGVVPVFGAVR--------------------------------------KGHWQVVELLLSHGVDVNVTDRQGRTPLMVSSSEGHLQTAAFLHSQGASVAAVDREGLSALSWACLKGRLPVVRFLLENGAAIEHADKSGRTPLDLAAFYGDENVIDCLIEHGASID 1081          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 172.555 bits (436), Expect = 7.019e-44
Identity = 164/607 (27.02%), Postives = 273/607 (44.98%), Query Frame = 2
Query: 1634 NVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAK-------PNE-----------IDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISV------------------LQSQYDCF------QVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLID-VGA-RIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSC-MRTPYKVAYKGNHIKICNLL 3307
            N +   L  +    S   +  L+ +  D+N  ++N  T L VAA N        ++   ++  + D+ G +AL  A +NG+ E+V  L+++  + +      R A+  AA++G T++V +L++ G+++  +D +G +PL AA+      +VKLLL+ G  ID  ++ G +A+ VA  C N       ++  L+    N+N  +  G   L  AA      LC ++L+ N AD+++     +SPL   A  G F   + L+  G  I C D+ G + L I+A  G+  +++ L+  G D   R   G  PLHLA++ G  +C   LL +G K        NE            D  GR  L  +   G ++    L+    + +N K     T+L  A  N   + V  L++ GA    QD    TPLH +                   +QS  D        Q+ L+H L  + +     D  G T +H A+  G    ++ L++  GA + D K     + LHL A+ G +   ++LI   +++D     G T L +A+  GH +   +LI  GA+V   +    RT    A    H++   LL
Sbjct:   70 NKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVA--CYNGQDV---VVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGAD-MNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADP-GIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLL 669          

HSP 2 Score: 162.54 bits (410), Expect = 9.233e-41
Identity = 140/498 (28.11%), Postives = 236/498 (47.39%), Query Frame = 2
Query: 1859 AIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGAR------------------IDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTP--YKVAYKGN 3283
            AI+NG  + V+ L+ +K + +   ++ RT L  AA++G  +I+ LL+ SG+ +N +D++  TPL  A        V+ LL + A ++ +D + ++ + VAA   N   K  + I  +L  +   N+ D  G   L  AA+ G   +   L+   A+I   D  DR  +  AA+ G+ EVV++L+  GA + C DK G+S L  ++S G   ++  LL  G+D    ++FG T LH+A   GQ   VN L+  GA  N+ +  G  PL  +    +  +  +L+      VN +S DG + L +  ++      + L+  G +   +D +G TPLHI+   ++Y     LL ++L  N  +       G   +H+A+  G  +  + L+  G +                  ID  D  GRT LH  A  G  +   LL+  G ++++      TAL  A+   +++    L+  GA +   D    TP  Y  A  G+
Sbjct:   13 AIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAA--NRAVKCAEAIIPMLSSS---NVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADVNVQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCEDRDGNTPLHIA---ARYG--HELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGD 500          

HSP 3 Score: 152.525 bits (384), Expect = 1.208e-37
Identity = 163/588 (27.72%), Postives = 266/588 (45.24%), Query Frame = 2
Query: 1685 TISQ-LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLS--QK----------------IDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSG--FSVLSISASQGNSQILSELLARGLDEMH-RDNFGCTPLHLASVEGQYECVNLLLKSGAK------PNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWP--ESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDV----GARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLIN-FGANVCHI-DSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            T SQ LI NG +I+C + +G T L +AA+ G   + N L+++GA+       G   L LA  +G+ +  + LLS  QK                 + D     GRT L  AA  G  + + LLLS+G+++NK+D   RT L  A   C+   V  L+ +GA ++ QD    + +  AA      S     +   +     I++           +    +++  + LL  +AD  I D+   +P+  AA  G+   + LLL    A     KSG   S L ++A  G+   L E+L R L ++  R+  G TPL LA++ G  +CV +L++ GA       P+      R  L  +   G+++    L++     E+V+    +  T + +AV N   +    LLS+GA    +DN G T LH        +C  +LLQ S+     +++  +  GR  +H+A+  G   I+ +L+      GA I L D  G T LH   + G     +LL++  +   +      + L  A    +     +LI+ FGA + +  D+  R P   A   +H++   LL  H ALV+
Sbjct:  318 TRSQTLIQNGGEIDCEDRDGNTPLHIAARYGHELLINTLITNGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSAGQKYSIVSSFSNEHVLSAGFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVH----------STCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGAL-EVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDR-----RTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSV-----DVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDV-FQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVN 883          

HSP 4 Score: 132.88 bits (333), Expect = 1.441e-31
Identity = 147/593 (24.79%), Postives = 254/593 (42.83%), Query Frame = 2
Query: 1709 GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLS----------QKIDP----DFTGNDGRT-------ALRTAAWI------GRTDIVL-LLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNN-GAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD----RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGF---SVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPES---VNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLI----------DFGINI---------DETCSQGA------------------TALSIASQEGHLEICNLLINFGANVCHIDSCMRTP 3259
            G +I+  +E G T L  AA  G     NLLLS+GA+    D+   +AL  A  N  ++ V  L++          ++  P         DG T        +   ++I          I L  LL   ++   QD +G TP+  A      V ++LLL   GA+     +DG+S   ++ L L +   +   +++L+R   ++++ +  G   L +AA  G     ++L+++ A + + +    R+ L +AA +G+ E +RLL+    +   +D S     + + ++   G++   S LL++G     +DN GCT LH  +  G  ECV+LLL+     +  +  GR PL ++   G++ I   L+     S   +     +G T L  A  N     VE LL        Q+ N  +PLH +VL     C ++L+    T     INC D  GR  +H A++    E ++ L+   A +++ D  G++ L +    G     ++L+          D   N           ETC+                     T L +A++ G   +  +L+N GA++  +D    TP
Sbjct:  411 GFEIDTPDEFGRTCLHAAASGGNVECLNLLLSTGADMNKKDRFERTALHYAAANCNVQCVVTLVASGARLNEQDERRCTPLHYAAAADGDGSTVSFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAF----QSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDV-FQKQEGNAFSPLHCAVLNDNESCAEMLID---TFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGASILAVDHNGYTP 995          

HSP 5 Score: 131.339 bits (329), Expect = 3.787e-31
Identity = 89/300 (29.67%), Postives = 146/300 (48.67%), Query Frame = 2
Query: 2333 IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEIC-NLLINFGANV 3229
            +  ++PL+ A F+G+ + VR L++    ++ +D    + L  +A  G ++I+  L+  G     +DN   TPLH A        V  LLK  A  N  D + + PL ++     +K AE +I +   S N     G T+L  A  N   E V FL+++GA+    D   R  +H +      +  +VL+ H       ++ C D  G + +H AS  G   ++K L+D+G  ID  ++ G T LH+  + G   +   LID G N+++   +G T L  A+   H  +C  LL+N GA+V
Sbjct:    4 LCSQAPLIQAIFNGDPDEVRSLIYKKEEVNAVDDEKRTPLHAAAYLGEAEIIELLVLSGARVNSKDNKWLTPLHRACASRSEAAVQTLLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSS-NVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGA-----DVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELIDCGANVNQANERGFTPLHFAAASTHGALCLELLVNNGADV 297          

HSP 6 Score: 105.531 bits (262), Expect = 3.311e-23
Identity = 123/514 (23.93%), Postives = 206/514 (40.08%), Query Frame = 2
Query: 1643 SFSLFESIRQNSY----NTISQ-------LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGW--SALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNIN-KQDSEGRTPLMAATYKCDYVMVKLLLNNG---AYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEV----VRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLA-----------------------RGLDEMHRDNFGCT-----------PLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA------------------------------------VLNRLPEFVEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLL 2899
            SFS++ +I   SY    +T S+       L+    D    ++ G T +  AA  G      LLL          K G   S L LA ++G+   +++L+   +D D    +G T L  AA  G TD V +L+  G+++  K+  + RT L +A        ++LL+ N      +D  D + ++ + +A       + + D   LLL    +IN  DN+G   L   A  G +    +LL+ + D+ + +R   +PL  AA  G+  +    V+  +  GA I   D++G++ L  +   G+   +  LL                            EM  D FG             PLH A+     EC+ LLL   A  N  D  G+ PLM++C+ G     E L++     +  +  + +T+L +A                                      + +   V+ LL++GA     D+NG TP L  +  +S  +C  ++L
Sbjct:  505 SFSVYNTISVQSYIDVHSTCSEAQICLEHLLRKDADPGIQDKQGYTPVHYAAAYGHRVCLELLLEQPGAFQSDGKSGHPISPLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVENADSNEAVDASDANEQTPVMLAV-----GNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALVQAAMASGAPIPLTDRNGYTPLHWACYNGHDGCVELLLELDVFQKQEGNAFSPLHCAVLNDNESCAEMLIDTFGAKIINCPDAKGRMPLHAAAFTDHVECLQLLLSHNALVNVTDMAGKSPLMMACENGQATAVEVLVNSTKTDLKLQDANRNTALHLACSRSHETCALLILDKLDDLNLINATNAALQTPLHLAARSGMATVVQVLLNKGASILAVDHNGYTPALSCAPNKSVAECLSLIL 1013          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672])

HSP 1 Score: 79.7221 bits (195), Expect = 1.448e-15
Identity = 63/241 (26.14%), Postives = 103/241 (42.74%), Query Frame = 2
Query: 2468 QGNSQILSELLARGLDEMHRDNFGC---TPLHLASVEGQYECVNLLLKSGAKPN-EIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQ 3178
            +G+   + E++   L E++ D       T LH AS+  +   V+ L+  GA  N         PL  + + GY+ I                                  V+FLL  GAD T+ D+ G   LH+SV  S       +L + ++  + +I+C D  GRT++  A+++GD   +  L+  GA I + D EG T LH G  +G   + + LI  G +  +    G    +IA +
Sbjct:   83 RGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYI----------------------------------VDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQE 289          

HSP 2 Score: 65.4698 bits (158), Expect = 3.510e-11
Identity = 51/168 (30.36%), Postives = 81/168 (48.21%), Query Frame = 2
Query: 1673 NSYNTISQLIINGLDINC-VNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKI-----DPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA 2158
            N  + +  L+  G D+N        T L  AA+ G+  I + LL  GA+  M D  G++ L L++ +  I +V  +L   +     D D     GRT+L  AA+ G +  V  LL  G++I   D+EG TPL   T K    ++K L+ +GA    + + G+    +A
Sbjct:  120 NRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIA 287          

HSP 3 Score: 55.8398 bits (133), Expect = 3.537e-8
Identity = 59/217 (27.19%), Postives = 93/217 (42.86%), Query Frame = 2
Query: 2000 SSGSNINKQDSEGRTPLMA----ATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNE-GMNMLLIAAYEGSKLLCKILLENNADIDIVDRSP---LMAAAFSGNFEVVRLLLFWGAA-----IHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGA 2611
            S    +N  D     PL+     A  + D   VK +++ G  ++  +NDG S   +  L   S +  L ++  L+    ++N          L  AA  G   +   LL++ AD  + D      L  +  S N  +V  +LF   +     I C D  G + L  +A QG+S  ++ELL  G      D  G TPLH  +V+GQ   +  L++ GA
Sbjct:   57 SGNEQVNHNDEAEEDPLLTRYHTACQRGDLATVKEMIH-GKLLE-VNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGA 271          

HSP 4 Score: 53.9138 bits (128), Expect = 1.416e-7
Identity = 56/223 (25.11%), Postives = 93/223 (41.70%), Query Frame = 2
Query: 2657 SCQEGYIKIAEKLIDVWPESVN--FKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNN-GRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEII---------KSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHI 3289
            +CQ G +   +++I      VN    S +  T L  A +N     V+FL+S+GAD   +      TPLH +           LL+H     +      DD G   +H++    +  ++         K L+D    ID +D +GRT L   A+QG       L+ FG +I    ++G T L   + +G   +   LI  GA+        +  + +A + N +
Sbjct:   80 ACQRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINNRLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMT-----DDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLD----IDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQEMNTV 293          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 67.781 bits (164), Expect = 7.235e-13
Identity = 58/205 (28.29%), Postives = 95/205 (46.34%), Query Frame = 2
Query: 2645 PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQD---NNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN-IDETCSQGATALSIASQEGHLEICNLLI-NFGANVCHIDS 3244
            PL  +C E      ++L+   P  +  K  DG   L  +V  +  E   FLLS+  +  + D   ++G TP HI+      +  + L    L  ++N I    + G T +H+A  K   E+ + LI+ GA + +KD   +  LH  A  G  ++ +LL   G + ++    QG T L  A  EGH +   LL+  +GA    +D+
Sbjct:    5 PLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKIT---NQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDN 206          

HSP 2 Score: 51.9878 bits (123), Expect = 1.174e-7
Identity = 51/223 (22.87%), Postives = 99/223 (44.39%), Query Frame = 2
Query: 1643 SFSLFESIRQNSYNTISQLIINGLDINCV-NENGTTSLMVAAQNGFTSICNLLLSSGANQLM---IDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTG--NDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNG-AYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSK 2290
            ++ L ++  +N +  + +L+ +   +    +++G   L  +       I + LLS   N  +    D  GW+   +A   G +EVVK L  + + PD     N G T L  A      ++   L+ +G+++  +D   + PL  A       +++LL   G + ++ QD  G + +F A    + ++  L    L+ +Y    +L+DN+G     +A  E  K
Sbjct:    3 NYPLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVL----LVEKYGAEYDLVDNKGAKAEDVALNEQVK 221          

HSP 3 Score: 50.447 bits (119), Expect = 4.431e-7
Identity = 42/140 (30.00%), Postives = 67/140 (47.86%), Query Frame = 2
Query: 2429 DKSGFSVLSISASQGNSQILSELLARGL--DEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSR-GADATIQDNNG 2839
            D SG++   I+ S GN +++  L  R L  D     N G T LHLA  +  +E    L+++GA     D   +IPL  +   G +K+ E L  +   +VN++   G T L  A+     +    L+ + GA+  + DN G
Sbjct:   69 DDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKG 208          

HSP 4 Score: 46.9802 bits (110), Expect = 6.064e-6
Identity = 47/177 (26.55%), Postives = 90/177 (50.85%), Query Frame = 2
Query: 1991 LLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLL--LNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLL--RYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWG-AAIHCIDKSGFSVL--SISASQGNSQIL 2488
            LL S  S + ++D +GR PL  + +++   +   LL  + N    D+ D+ G +   +A    N     L+++K L       ++N I N+G+  L +A  +    + + L+EN A + I D+    PL  AA  G+ +++ LL   G +A++  DK G++ L  +++   G++ +L
Sbjct:   21 LLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGN-----LEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVL 192          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560])

HSP 1 Score: 62.3882 bits (150), Expect = 3.588e-10
Identity = 58/204 (28.43%), Postives = 102/204 (50.00%), Query Frame = 2
Query: 1958 AAWIGRTDIVLLLLSSGS-NINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKY--LDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILL------ENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDN 2533
            AA  G   +V  ++ SG+ +IN    +  + L  A     + + K LL  GA      N  ++A    A  L+  ++Y  + I+ LLL++  +  L D +G+N++  + Y  + LL   +L       +N DID  D   R+PL+ AA+ G+F  V LLL +G+ +   D  GF+ L  +   G+ +++ +L+  G +   R+N
Sbjct:   57 AAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKFLLLRGA------NPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNN 254          

HSP 2 Score: 60.8474 bits (146), Expect = 1.086e-9
Identity = 58/245 (23.67%), Postives = 101/245 (41.22%), Query Frame = 2
Query: 2459 SASQGNSQILSELLARGLDEMHRDNFG-CTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNIN-NINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQ-ICQLLIDFGINIDETCSQGATALSIASQEG 3184
            +A  G+ +++ +++  G  +++ D     + LH A +  ++     LL  GA PN+    G                                 G+T+L  A        V+ LL  GAD T++D  G   +H SV  S       +L   + +N N +I+  D++ RT +  A+++GD   ++ L+  G+ +   DN G   LH     G  + IC L++  G N  E  +Q      +A   G
Sbjct:   57 AAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKFLLLRGANPNQAAGPG---------------------------------GATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILS-GANFYERNNQKQDCFDLAEGMG 267          

HSP 3 Score: 58.5362 bits (140), Expect = 5.575e-9
Identity = 57/193 (29.53%), Postives = 93/193 (48.19%), Query Frame = 2
Query: 1760 AAQNGFTSICNLLLSSGANQLMIDK-DGWSALRLAIWNGYIEVVKLLLSQKIDPD-FTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLL------NNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEG-SKLLCKILL 2311
            AA++G   +   ++ SGA  +  D+ D  S L  A  N    V K LL +  +P+   G  G TAL  AA  G   IV LLL  G++   +D +G   +  + Y  + ++V  +L      N+   ID +DN+ R+ +  AA        +L  ++LLL++   +   DN G N L  A   G  +++C ++L
Sbjct:   57 AAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAY----QGDFL-TVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLIL 244          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374])

HSP 1 Score: 62.3882 bits (150), Expect = 4.185e-10
Identity = 39/100 (39.00%), Postives = 55/100 (55.00%), Query Frame = 2
Query: 2942 DDDGRTAIHIASWKGDGEIIKSLIDVGA-RIDLKDNEGRTGLHLGAWQGCYQICQLLIDFG--INIDETCSQGATALSIASQEGHLEICNLLINFGANVC 3232
            D  GRT + IA  KG  +++K +I+ G   I+ +DN G T LH  A QG  +I +LLI+ G  +NI      G T L  AS  GHL++   L+  GA+  
Sbjct:  327 DSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPT 426          

HSP 2 Score: 53.1434 bits (126), Expect = 2.949e-7
Identity = 34/98 (34.69%), Postives = 54/98 (55.10%), Query Frame = 2
Query: 3038 KDNEGRTGLHLGAWQGCYQICQLLIDFG-INIDETCSQGATALSIASQEGHLEICNLLINFGA--NVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            +D+ GRT L +   +G Y + + +I+ G  +I++  + G TAL  A+ +GH+EI  LLI  GA  N+  I+    TP   A    H+ +   L K+GA
Sbjct:  326 RDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGA 423          

HSP 3 Score: 51.2174 bits (121), Expect = 9.329e-7
Identity = 40/112 (35.71%), Postives = 63/112 (56.25%), Query Frame = 2
Query: 2519 MHRDNFGCTPLHLASVEGQYECVNLLLKSGAKP-NEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYD--GSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRT 2845
            ++RD+ G T L +A  +G+Y+ V  +++ G    N+ DN G   L  +  +G+I+I E LI+   + VN KS +  G T L  A  N   + V++LL  GAD TI++  G T
Sbjct:  324 IYRDSGGRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGAD-VNIKSIEMFGDTPLIDASANGHLDVVKYLLKNGADPTIRNAKGLT 434          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672])

HSP 1 Score: 54.299 bits (129), Expect = 7.025e-8
Identity = 42/149 (28.19%), Postives = 72/149 (48.32%), Query Frame = 2
Query: 2468 QGNSQILSELLARGLD---EMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDN--HGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLL 2899
            +GN  I+  LL R  D    +  +N G + LH AS  G+Y     L++ G   +E+     G   + L+  +G+ +    L+  +P  +N +  +G   + IA +N   + +  L+  GAD  + D NG TPLH+ +      C ++LL
Sbjct:   15 EGNLLIVKRLLRRNPDLLTNIDSEN-GWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLL 162          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Match: EDO42780 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0Q6])

HSP 1 Score: 98.2117 bits (243), Expect = 3.156e-23
Identity = 67/180 (37.22%), Postives = 85/180 (47.22%), Query Frame = 2
Query: 2537 GCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGA 3076
            G TPL +A   G  ECV  L  SGA  N  DN GR PL L+  +G+                    DG               V +LL+ GAD+TI DN GR PLH +   S      +LLQH  + +IN     D +G TA+H A++ G  E +K LID  A I  +D EG+T LH  A
Sbjct:   48 GMTPLFVAVSTGNLECVQFLATSGASLNTRDNLGRTPLALAAYQGW-------------------NDG---------------VYYLLAVGADSTIVDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINK---QDGEGMTALHWAAYHGRAEHVKLLIDHDAEIVARDTEGKTALHWAA 190          

HSP 2 Score: 70.4774 bits (171), Expect = 1.312e-13
Identity = 51/153 (33.33%), Postives = 73/153 (47.71%), Query Frame = 2
Query: 2735 DGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLID--VGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGH 3187
            +G T L +AV     E V+FL + GA    +DN GRTPL ++  Q   D    LL       I     +D+ GR  +H A++   G  +  L+       I+ +D EG T LH  A+ G  +  +LLID    I    ++G TAL  A+Q  H
Sbjct:   47 EGMTPLFVAVSTGNLECVQFLATSGASLNTRDNLGRTPLALAAYQGWNDGVYYLLAVGADSTI-----VDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINKQDGEGMTALHWAAYHGRAEHVKLLIDHDAEIVARDTEGKTALHWAAQVSH 194          

HSP 3 Score: 68.5514 bits (166), Expect = 6.009e-13
Identity = 45/147 (30.61%), Postives = 71/147 (48.30%), Query Frame = 2
Query: 2417 IHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWP-ESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLH 2854
            I  ++  G + L ++ S GN + +  L   G     RDN G TPL LA+ +G  + V  LL  GA    +DN GR+PL  +      +    L+   P + +N +  +G T+L  A  +   E V+ L+   A+   +D  G+T LH
Sbjct:   41 IEALNSEGMTPLFVAVSTGNLECVQFLATSGASLNTRDNLGRTPLALAAYQGWNDGVYYLLAVGADSTIVDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINKQDGEGMTALHWAAYHGRAEHVKLLIDHDAEIVARDTEGKTALH 187          

HSP 4 Score: 64.3142 bits (155), Expect = 1.732e-11
Identity = 53/162 (32.72%), Postives = 84/162 (51.85%), Query Frame = 2
Query: 1826 IDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNN--GAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAA---YEGSKLL 2296
            ++ +G + L +A+  G +E V+ L +     +   N GRT L  AA+ G  D V  LL+ G++    D+ GR PL AATY      V LLL +    +I+ QD +G +A+  AA   +  +++   +KLL+ ++  I   D EG   L  AA   ++G KL 
Sbjct:   44 LNSEGMTPLFVAVSTGNLECVQFLATSGASLNTRDNLGRTPLALAAYQGWNDGVYYLLAVGADSTIVDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINKQDGEGMTALHWAA--YHGRAEH---VKLLIDHDAEIVARDTEGKTALHWAAQVSHDGLKLF 200          

HSP 5 Score: 56.6102 bits (135), Expect = 7.753e-9
Identity = 45/146 (30.82%), Postives = 67/146 (45.89%), Query Frame = 2
Query: 1622 IEATNVYSFS-LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGR--TALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL 2050
            IEA N    + LF ++   +   +  L  +G  +N  +  G T L +AA  G+      LL+ GA+  ++D  G   L  A +      V LLL           DG   TAL  AA+ GR + V LL+   + I  +D+EG+T L
Sbjct:   41 IEALNSEGMTPLFVAVSTGNLECVQFLATSGASLNTRDNLGRTPLALAAYQGWNDGVYYLLAVGADSTIVDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINKQDGEGMTALHWAAYHGRAEHVKLLIDHDAEIVARDTEGKTAL 186          

HSP 6 Score: 56.225 bits (134), Expect = 9.272e-9
Identity = 43/155 (27.74%), Postives = 74/155 (47.74%), Query Frame = 2
Query: 2828 DNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDF--GINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNH 3286
            ++ G TPL ++V     +C Q L     +     +N  D+ GRT + +A+++G  + +  L+ VGA   + DN GR  LH   +    +   LL+      +I++   +G TAL  A+  G  E   LLI+  A +   D+  +T    A + +H
Sbjct:   45 NSEGMTPLFVAVSTGNLECVQFLATSGAS-----LNTRDNLGRTPLALAAYQGWNDGVYYLLAVGADSTIVDNCGRLPLHAATYYSSGRTVGLLLQHLPSDHINKQDGEGMTALHWAAYHGRAEHVKLLIDHDAEIVARDTEGKTALHWAAQVSH 194          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84])

HSP 1 Score: 88.5817 bits (218), Expect = 1.333e-19
Identity = 69/226 (30.53%), Postives = 105/226 (46.46%), Query Frame = 2
Query: 2252 MNMLLIAAYEGSKLLCKILLENNADI---DIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEG-QYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGS---TSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHS 2908
            M  L +A   G    CK LL    D+   D  DR+PL  AA SGN   + LL+      +CIDK G + L  +A   N + +  L+  G +    D   CTPLH A+      +CV  LL++    ++ D  G   +  +   G+    E L+DV    +  +S  GS   T L +A  +     +E L+    D  ++D+ GRTPL ++  Q   +C + LL H+
Sbjct:    1 MQPLHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRS-SGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHN 225          

HSP 2 Score: 80.1073 bits (196), Expect = 1.047e-16
Identity = 72/224 (32.14%), Postives = 112/224 (50.00%), Query Frame = 2
Query: 1751 LMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL-MAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEG---MNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLL 2401
            L +A   GFT  C  LL  G +    D +  + L  A  +G +  ++LL+SQ  D +     GR  L  AA       V+ L+++GSN+NK D++  TPL  AA    D   V+ LL N      +D  G SA+  A+    +N   L  I++LL  + N  L  + G   +  L +AA+ G K   ++L+++  D+++ D   R+PL  A+F G+ E V  LL
Sbjct:    4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYAS----ANGHKL-AIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLL 222          

HSP 3 Score: 75.8702 bits (185), Expect = 2.808e-15
Identity = 71/232 (30.60%), Postives = 104/232 (44.83%), Query Frame = 2
Query: 2546 PLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYD--GSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQS-QYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGR---TGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGA 3223
            PLHLA + G  EC   LL  G   N  DN+ R PL  +   G +   E L+    ++ +F   D  G   L  A  +     V  L++ G++    D    TPLH +        C + LL+     N  N +  D  G +A+H AS  G    I+ L+DV A   L  + G    T LHL A+ G     ++LI    +++    +G T L +AS +GH+E    L+N  A
Sbjct:    3 PLHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMS---QAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLR-----NRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNA 226          

HSP 4 Score: 75.0998 bits (183), Expect = 5.603e-15
Identity = 73/261 (27.97%), Postives = 114/261 (43.68%), Query Frame = 2
Query: 1850 LRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS-------PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGA 2611
            L LA   G+ E  K LL   +D +   N+ RT L  AA  G  + + LL+S   + N  D  GR PL  A    ++  V  L+  G+ ++  D    + +  AA    ++      ++ LLR   N +  D +G + +  A+  G KL  ++LL+ +A+ D+++RS       PL  AAF G+   + +L+             F+ L +                      RD+ G TPL LAS +G  ECV  LL   A
Sbjct:    4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAA----ASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSAN-DLLNRSSGSPCVTPLHLAAFHGHKPALEVLI-----------KSFNDLEV----------------------RDDRGRTPLDLASFQGHVECVECLLNHNA 226          

HSP 5 Score: 63.5438 bits (153), Expect = 5.355e-11
Identity = 59/231 (25.54%), Postives = 99/231 (42.86%), Query Frame = 2
Query: 2645 PLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKG-DGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGA---TALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGAL 3325
            PL L+C  G+ +  +KL+  +   VN K  +  T L  A  +     +E L+S+  D    D  GR PLH +   + + C       SL    +N+N  D    T +H A+    D + ++ L+        +D +G + +H  +  G     ++L+D   N     S G+   T L +A+  GH     +LI    ++   D   RTP  +A    H++    L  H A+
Sbjct:    3 PLHLACLGGFTECCKKLL-FYGVDVNAKDNNDRTPLHCAACSGNVNCLELLMSQAGDFNCIDKLGRNPLHYAAGHTNFRCVM-----SLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRGNASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLASFQGHVECVECLLNHNAM 227          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Match: EDO37520 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFL9])

HSP 1 Score: 90.8929 bits (224), Expect = 2.518e-18
Identity = 182/860 (21.16%), Postives = 323/860 (37.56%), Query Frame = 2
Query:  236 FKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNL---CNYPDRAFKNGILLPLLS-FPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVR---KFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKD----DNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLS-IQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLF---KIKYFL-VKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAF----HLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFS-LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGA---------YIDHQDNDGRSAIFV---------AALCLNSNSKYLDIIKLLLRYN-CNINLIDNEGMNMLLIAAYEGSKLLCKILLENN-------ADIDIVDRSP--------------------LMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLAR-GLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGA 2611
            F +  +   +L  H C +S   +   AKF+  +   +  S    E     +N     N+++ Q L    N P   F   +L PL     PP+  M++++D++DE +L              +T  KN      I +LLS   + FP W+ ++ T +K    +       +   +   D+ N  Y   DI+ Y+ +++ +       +   +   T E ++ L  +S G  +Y++++L+ +    L   + I  +P  +  +Y+   +  F   ++F+ V+ I  +++++ + L   +IY  +   + ++D  Y  D+L    ++  FL            H+SL  WL   +     +  +  +GH +LA Y   I  K +        F    H+     I K KG   A               LP E               +L  S +  N+  L+   S +  +T ++ + + + ++++  +  S +  F +      N ++ L   G ++  V  + +      A+N     C  L+            G+S L  A   G++E VK LL+ K+  D     G T ++ AA  GR  ++ +L   G+  + +      PL +A        V+ L   G           I       R    V          AL + S  +  DI+  +LR N   +   D  GM  L  A    S    K  LE         A  +++   P                    L  AA  G   ++  LL +GA     D  G   + ++A      +LS L+A   +D   RD  G TP H A        +  L K GA
Sbjct:  409 FSSYSMNIRVLGYHMCISSYRSTKEPAKFVRSVAAMI--SSVIKEYSYITLN-----NIYIQQKLDTCVNDPVDCFDQVVLSPLRHILDPPSNQMFLVVDALDECIL--------------ETGRKN-----EIVELLSKTLDQFPSWVKVLLTSQKVPSILTEFSSPHVSLVEIEQDNPNNLY---DIEQYVSHKLYEQSTVWSRMVSFLTGNTDERISSLIKRSEGNFLYIKVMLEYMQTEKLQGPEQINDVPKSVHRIYMSYLKRHFTE-EEFVPVRRILKVLVAAYEPLLTTDIYHVIRAKNQSSDSNYYYDILRTLDRLSMFLHFSRQRETVQVFHSSLSVWLTSNETLGGPFYVNRSNGHQSLAEYFLSIVEKNSQRFGYRLGFYLANHIFEGGTIAKHKGRFLA---------------LPSE---------------LLNSSDSTTNMTALHFAASTS--NTGVIGLLANHFSTVDCLDRNSRTPAFIAASTGQKNILAILFQKGANLFHVTNSLSKKHQQRAKNPVKD-CKRLMC-----------GYSLLHAAAQGGHVETVKFLLNHKLSKDAESGAGNTPMQIAAQYGRVSVMEVLHKHGAPCDPR------PLRSAAAGGHLPAVRFLHQAGVSDECVPCKYSISPSYMKQRVGKLVDDEYLWRCETALHIASAMEQPDIVSYILRSNDTALTCYDYLGMRPLHKAVVYNSIKSLKAFLEYGVSPLSRCAPAEVLSTYPEDKYKNIADYFEPCICRYNALHLAARHGRVNMIATLLEYGAKTGVFDACGSQAVHVAACYDQVGVLSLLVAEYKVDVNSRDLIGKTPFHYAVKCKAAGALKRLFKLGA 1188          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 85.8853 bits (211), Expect = 2.679e-17
Identity = 102/439 (23.23%), Postives = 180/439 (41.00%), Query Frame = 2
Query: 1745 TSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLS--------QKIDPDFTGNDG--RTALRTAAWIGRTDIVLLLLSSGSNINK-QDSEGRT--PLMAATYKCDYVMVKL-------------------------------LLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVV-------RLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMH-RDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQH 2905
            T L VA  +G   +   L+ +GA+   +D++  + L  A   G + V KLL            +    T  D   +TAL  A   G  D+  L L  G+N+N  ++S+ +T    +    +C +VM+K+                               ++   A I+ +D D  + + +AA        ++D ++ LL++  +I   D    N +  AA EG     +  LE+   +  +     +     G+ +++       +LLL  GA I   +    + +  +A  G  + +  LL R    ++  D    TPLHLA++ G    V  LL  G   +  +     PL  +   G +++A  L+D     V+      +T L +A      E V+ LLSR A+ T+ D+ GR  L I++  S  +    ++ H
Sbjct:    6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAA-----EKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLD-EDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIIDH 438          

HSP 2 Score: 83.1889 bits (204), Expect = 2.058e-16
Identity = 112/470 (23.83%), Postives = 197/470 (41.91%), Query Frame = 2
Query: 1943 TALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLL-----LNNG-----AYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLID---NEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID----VWP--ESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKI 3295
            T L  A   G   +   L+ +G+++   D E  TPL  A  + +  + KLL     L  G       +  QD + ++A+ +A         + D+ KL L    N+N +    N+  +  ++ A E   ++ K+             S L   +F  + ++ R +L +           F V+ I        I   +  R   E  RD    TPL +A+ +   + V  LL+ GA     D + R  +  +  EG ++  E  ++    VW   + V      G ++  I VL R+    + LL  GA    +++   TP+H +    +      LL+     +   IN  D    T +H+A+  G   ++K L+  G  ID ++    T L   A +G  ++  +L+D    +D T     T L +AS+EGH+E+  LL++  AN+   DS  R    +A + +H ++
Sbjct:    6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAV-----EGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKV-------------SKLKYLSFCKSEKIKREILLYF----------FDVVII--------ICYIIRFRACIEC-RDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVL-RISS--QLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKR----DPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEV 431          

HSP 3 Score: 83.1889 bits (204), Expect = 2.058e-16
Identity = 108/453 (23.84%), Postives = 189/453 (41.72%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLS--------SGANQLMIDKDG--WSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRT-------------------------------DIVLLL---LSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA------------------------LCLNS----NSKYLDIIK----LLLRYNCNINLIDNEGMNMLLIAAYEGS-KLLCKILLENNA---DIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDV--WPESVNFKSYDGS--TSLKIAVLNRLPEFVEFLLS 2803
            L+  G D+  ++E   T L  A   G   +  LL          S  ++++ D+D    +AL LA+  G+ ++ KL L +  + +               + R                                D+V+++   +   + I  +D +  TPLM A  K     V+ LL +GA I  +D + R+++F AA                        +CLN     ++K + +++    LLL     I+  ++E +  +  AA  G  + +C +L  + A   D D    +PL  AA  G+  VV+ LL+ G AI   + + ++ L  +A++G  ++ + LL         D    TPLHLAS EG  E V LLL   A     D+ GR  L ++ +  + ++A  +ID   W +++  +   G+  TS    ++ +LP    +LL 
Sbjct:   23 LVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIIDHVDWKQAMRNRCKLGNAITSPMRKLIKKLPGQKLYLLQ 475          

HSP 4 Score: 56.9954 bits (136), Expect = 3.023e-8
Identity = 53/160 (33.12%), Postives = 80/160 (50.00%), Query Frame = 2
Query: 1691 SQLII-NGLDINCVNENGTTSLMVAAQNG-FTSICNLLLSSGANQLMIDKDGWS--ALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVA 2158
            SQL++ NG  I+  N+   T +  AA+ G   ++C LL    A  ++ D+DG S   L LA  +G+  VVK LL +    D       T L  AA  G  ++  +LL   + ++  D    TPL  A+ +    MVKLLL+  A I   D+ GR+ + +A
Sbjct:  266 SQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPA--IINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIA 423          

HSP 5 Score: 55.8398 bits (133), Expect = 8.445e-8
Identity = 93/410 (22.68%), Postives = 156/410 (38.05%), Query Frame = 2
Query: 2252 MNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDN------HGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVE---FLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKS-------------------------------------LIDVGARIDLKDNEGRTGLHLGAWQG-CYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            M  L +A   G   + K L+E  AD+  +D    +PL  A   GN  V +LL F  A +    + G+S +S   +                   +D    T LHLA   G  +   L L+ GA  N +        H  I +   C    IK+++         + + S+  S  +K  +L    + V    +++   A    +D +  TPL I+  ++  D  + LLQH       +I   D + R ++  A+ +G  E +++                                     L+D GA ID K++E  T +H  A  G    +C LL      I++      T L +A+  GH  +   L+  G  +   ++ + TP   A     +++ N+L    A VD
Sbjct:    5 MTPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLL-FVAAEL----RGGWSTVSKMVTD------------------QDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVMIKVSK---------LKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHG-----ADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRISSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVD 377          

HSP 6 Score: 49.2914 bits (116), Expect = 8.120e-6
Identity = 32/91 (35.16%), Postives = 45/91 (49.45%), Query Frame = 2
Query: 1688 ISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTA 1960
            + +L+  G  I+  N    T L  AA  G   + N+LL   A     DK   + L LA   G++E+VKLLLS+K +   T + GR  L  A
Sbjct:  333 VKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIA 423          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Match: EDO34863 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN65])

HSP 1 Score: 80.1073 bits (196), Expect = 4.171e-17
Identity = 54/161 (33.54%), Postives = 82/161 (50.93%), Query Frame = 2
Query: 2744 TSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSL-THNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNE-GRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFG 3220
            T L IAV+   P+ V+ L+  GA+  + D NG+TP H++  +S   C   L   SL  +NI N      +G   +HIA  K D   I  L+  GA +D KD + G+T LH    +   QI + L+D   +I+ T   G + +++A++ G      LL N G
Sbjct:   15 TPLHIAVITDQPDIVKCLIYAGANPNLPDRNGQTPAHLACQRSAVRCLYELTSSSLLDYNIRNF-----EGLMPLHIAVAKRDKYAIALLVQNGANVDCKDGKSGKTPLHHAIERNDLQIIKTLLDMSADINATDFSGVSPMAMANRRGLANAVRLLGNRG 170          

HSP 2 Score: 75.0998 bits (183), Expect = 2.268e-15
Identity = 50/173 (28.90%), Postives = 87/173 (50.29%), Query Frame = 2
Query: 2528 DNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQD-NNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVG--ARIDL 3037
            +N   TPLH+A +  Q + V  L+ +GA PN  D +G+ P  L+CQ   ++   +L        N ++++G   L IAV  R    +  L+  GA+   +D  +G+TPLH ++ ++     + LL  S      +IN  D  G + + +A+ +G    ++ L + G   R+DL
Sbjct:   10 NNLRQTPLHIAVITDQPDIVKCLIYAGANPNLPDRNGQTPAHLACQRSAVRCLYELTSSSLLDYNIRNFEGLMPLHIAVAKRDKYAIALLVQNGANVDCKDGKSGKTPLHHAIERNDLQIIKTLLDMSA-----DINATDFSGVSPMAMANRRGLANAVRLLGNRGEDQRVDL 177          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Match: ankrd50l (ankyrin repeat domain 50 [Source:NCBI gene;Acc:101161928])

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 364/1068 (34.08%), Postives = 571/1068 (53.46%), Query Frame = 2
Query:  266 LASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDY-LGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEK------FGSLNDWSIDDIVKILFESINLVN--INKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLF-----ESIRQ--------NSYNTISQLII-NGLDINCVNENGTTSLMVAAQNGFTSICNLLL-------SSGANQLM--IDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS---KYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID-----------IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSG--AKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDF-GINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            LASHFC   D  S  L +F+  + + L  S +    Y   ++   + +     +    PD  FK  +L PLL  PPP + + ++ID++D     DY  GE +               S +I++LL+ + +  P WL LVC+ R++N+ + + F GFRK  LDDL K   +RD+Q YIL R+DKD  L++ +  +T ++LNLLHIKS G  ++LE VLDG+    + ++ I+ IPG L GLYLW+ + LF   + F  V+ + N++L+S K L+  +++ A+   D +  +   ++ L  +   L+          H S  EWL DVK CT KY C   +GH  LA+        L        A HL+ S    K K    A+W+L++D    V  + P            L      ++ ++L  S  +++  +++ + K           ++   + + +  T +    L       + RQ        + +  +++L++ +G D    +  G T + +A+  G   + +LLL          A Q+M   D +GW+ALR A W G+ E V LLL    D +   ++GRTALR AAW G  +IV  LL  G+ +NK DS+GRTPL+AA Y   +  V++LLN+ A +D  D DGR+A+ VAALC+ + +    + ++  LLL +N +    DN+GM  LL+AAYEG + + ++LLE  AD+D               +PL+A+A  G+ + V  LLFWGAA+  ID  G + L ++A++G+ +++  LL RGLDE H+D+ G TPLH A+ EG       L + G  A+  E+D  GR PL+L+ QEG+    + L+D     ++ ++YDG ++L  A L    +  E L+ RGAD  ++D  GR  L++ VL+   +   +L++         +   D +GRTA+H+ASW+G  EI+  L+  GA+ + +D+EGR  LH  AW G  ++ + L++  GI+++  C QGATALSIASQEGH  I  +L+  G+N  H+D   R+P KVA K  H  I  LLE +GA
Sbjct:   86 LASHFCQREDQRSTVLWRFVLGLVEQLRASPFLSSGYEDILSRESVVSALEPLSCQRSPDETFKRAVLQPLLELPPPAQTLMVVIDALD----VDYEPGEGVGK-------------SDSIAELLATNQHLLPSWLLLVCSVRRQNKAVCKMFSGFRKLCLDDLRKPPAVRDVQQYILCRLDKDAALRRQLTPDTADMLNLLHIKSGGCFLFLERVLDGVAAALVGLREIRDIPGTLNGLYLWLCQRLFPR-RLFAHVRPLLNVLLASPKPLTSLQLFTAVWTQDTSLSIQDFQNKLQTLSPLLISGPGCTKLLFHASFAEWLTDVKYCTQKYLCGRSEGHTMLAMALTLRGPDLDADSTCQLAAHLVCSG-HHKDKPSLLALWMLWAD----VPALPPSSSTALTTSLAQLPALVSQNVPQLLMRSGVILSGCLSEPDIKVGMLSLCEEGADLQQPFDSGLSVTKLLDCGLSVDQLGPADRQTLLAKAALDGFEKVAELLLKHGSDPLICDHQGQTPMTLASSQGHVKVLSLLLEWAKGQGPQTAAQMMEHADNEGWTALRSAAWGGHKEAVGLLLDAGADVNGCDSEGRTALRAAAWGGHEEIVFTLLDYGAQVNKADSKGRTPLIAAAYMGHHETVEVLLNHNAEVDLADGDGRTALSVAALCVPTAAGVQGFGEVASLLLEHNADPGHRDNDGMTPLLLAAYEGHEDVVELLLEAGADVDETAGLDGSVSAAAAVTPLLASAAMGHMKTVSRLLFWGAAVDAIDCEGRTALCLAAARGSLEVVRALLDRGLDENHKDDLGWTPLHAAACEGHRAVCAALTEHGSMARVGEMDIEGRTPLILAAQEGHWSTVKILLDRR-SPIDHRAYDGHSALSAAFLEGHTDVAELLMKRGADTDVRDAEGRPLLYLLVLEDHLNMASLLIEKGGV----PLESRDSEGRTALHVASWRGTVEIVDLLLKHGAKPNAQDSEGRPPLHSVAWTGHAKVGRRLLETDGIDVNLACHQGATALSIASQEGHANIVKMLLEKGSNPNHLDKYGRSPVKVAAKHGHFTIVRLLESYGA 1125          

HSP 2 Score: 130.954 bits (328), Expect = 3.837e-30
Identity = 105/353 (29.75%), Postives = 169/353 (47.88%), Query Frame = 2
Query: 1709 GLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGS--NINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEG------------MNM----------------------LLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFW-GAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIP 2647
            GLD +       T L+ +A  G     + LL  GA    ID +G +AL LA   G +EVV+ LL + +D +   + G T L  AA  G   +   L   GS   + + D EGRTPL+ A  +  +  VK+LL+  + IDH+  DG SA+  A L       + D+ +LL++   + ++ D EG            +NM                      L +A++ G+  +  +LL++ A  +  D   R PL + A++G+ +V R LL   G  ++     G + LSI++ +G++ I+  LL +G +  H D +G +P+ +A+  G +  V LL   GAKP     HG +P
Sbjct:  790 GLDGSVSAAAAVTPLLASAAMGHMKTVSRLLFWGAAVDAIDCEGRTALCLAAARGSLEVVRALLDRGLDENHKDDLGWTPLHAAACEGHRAVCAALTEHGSMARVGEMDIEGRTPLILAAQEGHWSTVKILLDRRSPIDHRAYDGHSALSAAFL-----EGHTDVAELLMKRGADTDVRDAEGRPLLYLLVLEDHLNMASLLIEKGGVPLESRDSEGRTALHVASWRGTVEIVDLLLKHGAKPNAQDSEGRPPLHSVAWTGHAKVGRRLLETDGIDVNLACHQGATALSIASQEGHANIVKMLLEKGSNPNHLDKYGRSPVKVAAKHGHFTIVRLLESYGAKP----YHGTVP 1133          

HSP 3 Score: 124.405 bits (311), Expect = 3.918e-28
Identity = 106/353 (30.03%), Postives = 166/353 (47.03%), Query Frame = 2
Query: 2336 VDRSPLMA-AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL--ARGLDEM-------HRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVL----QSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARID--------LKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALV 3328
             DR  L+A AA  G  +V  LLL  G+     D  G + +++++SQG+ ++LS LL  A+G           H DN G T L  A+  G  E V LLL +GA  N  D+ GR  L  +   G+ +I   L+D +   VN     G T L  A      E VE LL+  A+  + D +GRT L ++ L     +    F  +    L HN +  +  D+DG T + +A+++G  ++++ L++ GA +D        +      T L   A  G  +    L+ +G  +D    +G TAL +A+  G LE+   L++ G +  H D    TP   A    H  +C  L +HG++ 
Sbjct:  542 ADRQTLLAKAALDGFEKVAELLLKHGSDPLICDHQGQTPMTLASSQGHVKVLSLLLEWAKGQGPQTAAQMMEHADNEGWTALRSAAWGGHKEAVGLLLDAGADVNGCDSEGRTALRAAAWGGHEEIVFTLLD-YGAQVNKADSKGRTPLIAAAYMGHHETVEVLLNHNAEVDLADGDGRTALSVAALCVPTAAGVQGFGEVASLLLEHNADPGH-RDNDGMTPLLLAAYEGHEDVVELLLEAGADVDETAGLDGSVSAAAAVTPLLASAAMGHMKTVSRLLFWGAAVDAIDCEGRTALCLAAARGSLEVVRALLDRGLDENHKDDLGWTPLHAAACEGHRAVCAALTEHGSMA 892          

HSP 4 Score: 82.0333 bits (201), Expect = 2.869e-15
Identity = 66/216 (30.56%), Postives = 94/216 (43.52%), Query Frame = 2
Query: 2690 KLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASW-KGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPY-KVAYKGNHIKICNLLEKHGALVD 3331
            KL+D           D  T L  A L+   +  E LL  G+D  I D+ G+TP+ ++  Q       +LL+                         W KG G    + +     ++  DNEG T L   AW G  +   LL+D G +++   S+G TAL  A+  GH EI   L+++GA V   DS  RTP    AY G+H +   +L  H A VD
Sbjct:  528 KLLDCGLSVDQLGPADRQTLLAKAALDGFEKVAELLLKHGSDPLICDHQGQTPMTLASSQGHVKVLSLLLE-------------------------WAKGQGPQTAAQM-----MEHADNEGWTALRSAAWGGHKEAVGLLLDAGADVNGCDSEGRTALRAAAWGGHEEIVFTLLDYGAQVNKADSKGRTPLIAAAYMGHH-ETVEVLLNHNAEVD 712          

HSP 5 Score: 52.373 bits (124), Expect = 4.099e-6
Identity = 26/74 (35.14%), Postives = 43/74 (58.11%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDP 1918
            L  +G+D+N     G T+L +A+Q G  +I  +LL  G+N   +DK G S +++A  +G+  +V+LL S    P
Sbjct: 1054 LETDGIDVNLACHQGATALSIASQEGHANIVKMLLEKGSNPNHLDKYGRSPVKVAAKHGHFTIVRLLESYGAKP 1127          

HSP 6 Score: 51.6026 bits (122), Expect = 7.221e-6
Identity = 39/140 (27.86%), Postives = 72/140 (51.43%), Query Frame = 2
Query: 2948 DGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGIN---------IDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            D +T +  A+  G  ++ + L+  G+   + D++G+T + L + QG  ++  LL+++            ++   ++G TAL  A+  GH E   LL++ GA+V   DS  RT  + A  G H +I   L  +GA V++ +
Sbjct:  543 DRQTLLAKAALDGFEKVAELLLKHGSDPLICDHQGQTPMTLASSQGHVKVLSLLLEWAKGQGPQTAAQMMEHADNEGWTALRSAAWGGHKEAVGLLLDAGADVNGCDSEGRTALRAAAWGGHEEIVFTLLDYGAQVNKAD 682          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Match: ENSORLT00000031565.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 215.698 bits (548), Expect = 3.247e-56
Identity = 158/564 (28.01%), Postives = 270/564 (47.87%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  + + + + +  G+DI+  N+NG  +L +AA+ G   +   LL  GA      K G +AL ++   G  EVVK L+ +  D +    +G T L  AA     D+V  LL +G N +    +G TPL  A  +    +V +LL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +VRLLL  G+ I    + G + L  +A  G+   +  LL +G   + R   G +PLH+A+     ECV  LL+  A  +++       L ++   G+ ++ + L+D    + N ++ +G T L IA      + +E L+  GA       +G TP+H++      +   +LLQ+  + +++NI      G T++H+A+  G  E+++ L+  GA +D +  E +T LH+ +  G  +I QLL+    + D   + G T L I+++EG LE  ++L+  GA+         TP  V  K   + +  LL +  A  D
Sbjct:   50 SFLRAARAGNIDKVLEYLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKR-ANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIR-----GETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPD 598          

HSP 2 Score: 207.994 bits (528), Expect = 8.628e-54
Identity = 168/555 (30.27%), Postives = 253/555 (45.59%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            ++ + N +  L+  G  I+    +G T L  AA++G  +   +LL  GA  L   K+G S L +A    ++E VK LL  K   D    D  TAL  AA  G   +  LLL   +N N +   G TPL  A  K    +++LL+  GA I      G + I VAA        +L+I+ LLL+   + ++ +  G   L +AA  G   + + LL N A +D     D++PL  A+  G  E+V+LLL   A       +G++ L ISA +G  +  S LL  G         G TPLH+ S  G  +   LLL+  A P+    +G  PL ++                        YD     K+A+L         LL +GA       NG TPLHI+  ++Q D   VLLQ+         N L   G T +H+AS +G  ++   L+  GA+I++    G T LHL A +    + ++L+  G N+D     G T L +A   G+ ++ + L+  GA+V        TP   A +  +  I N+L +HGA
Sbjct:  283 KRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFM-----GHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAH----------------------YDNQ---KVALL---------LLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGA-----ETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGA 793          

HSP 3 Score: 197.208 bits (500), Expect = 1.728e-50
Identity = 173/622 (27.81%), Postives = 278/622 (44.69%), Query Frame = 2
Query: 1652 LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICN-----------------------------LLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ + ++N  + +  L+ NG + +   E+G T L +A Q G   + +                             LLL +  N  +  K G++ L +A   G + V  LLL++    DFT  +G T L  A+  G T++V LLL  GS I+ +  +G TPL  A        V++LL  GA +  +  +G S + +AA                            L + ++  +  + KLLL    N N     G   L IA  +    + ++L++  A I  V  S   P+  AAF G+  +V LLL  GA+    +  G + L ++A  G ++++  LL  G     R     TPLH+AS  G+ E V LLL+  A P+    +G  PL +S +EG ++ A  L++    S +  +  G T L +       +  + LL R A       NG TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   +I   L+  GA  +    +G T LHL + +G   +  LL+  G  I+     G TAL +A+QE  + +  +L+  GAN+ H      TP  VA    + K+ + L ++GA V+ K
Sbjct:  150 LYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAG-ASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVA---AHYDNQKVALL--LLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAK 765          

HSP 4 Score: 171.785 bits (434), Expect = 1.384e-42
Identity = 129/428 (30.14%), Postives = 206/428 (48.13%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            NG T L +A +     +  LL+  GA+   + + G + + +A + G++ +V LLL     PD +   G T+L  AA  G+ ++V  LL +G+ ++ +  E +TPL  A+      +V+LLL + A+ D    +G + + ++A         L+   +LL    + +L   +G   L + +  GS  + K+LL+  A  D   +   +PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G +       G TPLHLAS EG  +   LL+  GA+ N     G   L L+ QE  + +AE L+     +++ ++  G T L +A      + V FLL  GA    +  NG TPLH +  Q       VLLQH    N   +N     G TA+ IA   G   ++ +L
Sbjct:  405 NGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISA-----REGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNG-ANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVN-----GNTALGIARRLGYISVVDTL 821          

HSP 5 Score: 135.961 bits (341), Expect = 1.224e-31
Identity = 111/417 (26.62%), Postives = 187/417 (44.84%), Query Frame = 2
Query: 1547 KLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            K N  K   +   Y  +I +   + +   +V +F           N +  L+ NG   +  N  G TSL +AA+ G   +   LL +GA   M+D   ++  + L +A   G  E+V+LLL     PD    +G T L  +A  G+ +   +LL +G++ +    +G TPL   +      + KLLL   A  D    +G + + VAA                            L + +    +DI  +LL+Y    N +  +G+  L +A+ EG   +  +L+   A I++  +S L A   AA      V  +L+  GA +    K G++ L ++   GN++++S LL  G     +   G TPLH A+ +G    +N+LL+ GAKPN +  +G   L ++ + GYI + + L
Sbjct:  415 KKNRVKVMELLVKYGASIQAVTESGLTPIHVAAF-------MGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGA---MVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 821          

HSP 6 Score: 129.413 bits (324), Expect = 1.236e-29
Identity = 105/398 (26.38%), Postives = 187/398 (46.98%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L +   ++D+++ LL     ++    +G   L I++  G   + K L++  ADI+   +   +PL  AA   + +VVR LL  G       + GF+ L+I+  QG++Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G + +A  L++    +V+F + +G T L +A        V  LL RG+    +  +G TPLH +         +VLL+         +     +G + +H+A+     E +K L+   A +D    +  T LH+ A  G Y++ +LL+D   N +     G T L IA ++  +++  LL+ +GA++  +     TP  VA    H+ I  LL ++GA  D  N+
Sbjct:   83 ALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRG-AAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAP-----LLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNI 470          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458])

HSP 1 Score: 215.312 bits (547), Expect = 4.634e-56
Identity = 158/564 (28.01%), Postives = 270/564 (47.87%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            S   + R  + + + + +  G+DI+  N+NG  +L +AA+ G   +   LL  GA      K G +AL ++   G  EVVK L+ +  D +    +G T L  AA     D+V  LL +G N +    +G TPL  A  +    +V +LL         +ND +  + + AL + +         LLL+ + N ++    G   L IAA+ G+  +  +LL   A +D   R   +PL  A+  GN  +VRLLL  G+ I    + G + L  +A  G+   +  LL +G   + R   G +PLH+A+     ECV  LL+  A  +++       L ++   G+ ++ + L+D    + N ++ +G T L IA      + +E L+  GA       +G TP+H++      +   +LLQ+  + +++NI      G T++H+A+  G  E+++ L+  GA +D +  E +T LH+ +  G  +I QLL+    + D   + G T L I+++EG LE  ++L+  GA+         TP  V  K   + +  LL +  A  D
Sbjct:   35 SFLRAARAGNIDKVLEYLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILL---------ENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKR-ANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIR-----GETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPD 583          

HSP 2 Score: 207.608 bits (527), Expect = 1.054e-53
Identity = 168/555 (30.27%), Postives = 253/555 (45.59%), Query Frame = 2
Query: 1667 RQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            ++ + N +  L+  G  I+    +G T L  AA++G  +   +LL  GA  L   K+G S L +A    ++E VK LL  K   D    D  TAL  AA  G   +  LLL   +N N +   G TPL  A  K    +++LL+  GA I      G + I VAA        +L+I+ LLL+   + ++ +  G   L +AA  G   + + LL N A +D     D++PL  A+  G  E+V+LLL   A       +G++ L ISA +G  +  S LL  G         G TPLH+ S  G  +   LLL+  A P+    +G  PL ++                        YD     K+A+L         LL +GA       NG TPLHI+  ++Q D   VLLQ+         N L   G T +H+AS +G  ++   L+  GA+I++    G T LHL A +    + ++L+  G N+D     G T L +A   G+ ++ + L+  GA+V        TP   A +  +  I N+L +HGA
Sbjct:  268 KRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFM-----GHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAH----------------------YDNQ---KVALL---------LLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGA-----ETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGA 778          

HSP 3 Score: 196.823 bits (499), Expect = 2.527e-50
Identity = 173/622 (27.81%), Postives = 278/622 (44.69%), Query Frame = 2
Query: 1652 LFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICN-----------------------------LLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            L+ + ++N  + +  L+ NG + +   E+G T L +A Q G   + +                             LLL +  N  +  K G++ L +A   G + V  LLL++    DFT  +G T L  A+  G T++V LLL  GS I+ +  +G TPL  A        V++LL  GA +  +  +G S + +AA                            L + ++  +  + KLLL    N N     G   L IA  +    + ++L++  A I  V  S   P+  AAF G+  +V LLL  GA+    +  G + L ++A  G ++++  LL  G     R     TPLH+AS  G+ E V LLL+  A P+    +G  PL +S +EG ++ A  L++    S +  +  G T L +       +  + LL R A       NG TPLH++   + YD  +V L   L     + +    +G T +HIA+ K   +I   L+  GA  +    +G T LHL + +G   +  LL+  G  I+     G TAL +A+QE  + +  +L+  GAN+ H      TP  VA    + K+ + L ++GA V+ K
Sbjct:  135 LYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAG-ASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVA---AHYDNQKVALL--LLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAK 750          

HSP 4 Score: 171.014 bits (432), Expect = 2.092e-42
Identity = 129/428 (30.14%), Postives = 206/428 (48.13%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSL 3010
            NG T L +A +     +  LL+  GA+   + + G + + +A + G++ +V LLL     PD +   G T+L  AA  G+ ++V  LL +G+ ++ +  E +TPL  A+      +V+LLL + A+ D    +G + + ++A         L+   +LL    + +L   +G   L + +  GS  + K+LL+  A  D   +   +PL  AA   N +V  LLL  GA+ H   K+G++ L I+A +    I + LL  G +       G TPLHLAS EG  +   LL+  GA+ N     G   L L+ QE  + +AE L+     +++ ++  G T L +A      + V FLL  GA    +  NG TPLH +  Q       VLLQH    N   +N     G TA+ IA   G   ++ +L
Sbjct:  390 NGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISA-----REGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNG-ANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVN-----GNTALGIARRLGYISVVDTL 806          

HSP 5 Score: 135.576 bits (340), Expect = 1.560e-31
Identity = 111/417 (26.62%), Postives = 187/417 (44.84%), Query Frame = 2
Query: 1547 KLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMID---KDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA----------------------------LCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKL 2695
            K N  K   +   Y  +I +   + +   +V +F           N +  L+ NG   +  N  G TSL +AA+ G   +   LL +GA   M+D   ++  + L +A   G  E+V+LLL     PD    +G T L  +A  G+ +   +LL +G++ +    +G TPL   +      + KLLL   A  D    +G + + VAA                            L + +    +DI  +LL+Y    N +  +G+  L +A+ EG   +  +L+   A I++  +S L A   AA      V  +L+  GA +    K G++ L ++   GN++++S LL  G     +   G TPLH A+ +G    +N+LL+ GAKPN +  +G   L ++ + GYI + + L
Sbjct:  400 KKNRVKVMELLVKYGASIQAVTESGLTPIHVAAF-------MGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGA---MVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQHGAKPNAVTVNGNTALGIARRLGYISVVDTL 806          

HSP 6 Score: 129.028 bits (323), Expect = 1.536e-29
Identity = 105/398 (26.38%), Postives = 187/398 (46.98%), Query Frame = 2
Query: 2159 ALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            AL L +   ++D+++ LL     ++    +G   L I++  G   + K L++  ADI+   +   +PL  AA   + +VVR LL  G       + GF+ L+I+  QG++Q++S LL        +       LH+A+ +   +   LLL++    +     G  PL ++   G + +A  L++    +V+F + +G T L +A        V  LL RG+    +  +G TPLH +         +VLL+         +     +G + +H+A+     E +K L+   A +D    +  T LH+ A  G Y++ +LL+D   N +     G T L IA ++  +++  LL+ +GA++  +     TP  VA    H+ I  LL ++GA  D  N+
Sbjct:   68 ALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLENDT----KGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRG-AAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAP-----LLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNI 455          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 214.927 bits (546), Expect = 4.721e-56
Identity = 169/598 (28.26%), Postives = 277/598 (46.32%), Query Frame = 2
Query: 1688 ISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPD-----------FTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADID---IVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYEC---------------------------------VNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKN 3340
            +++L+  G ++N  ++ G T L +AAQ     +   LL +GANQ +  +DG++ L +A+  G+  VV LL+S                 F    G T L  AA     ++  LLL+ G+N      +  TPL  A     + ++++LL+NGA I  +  +G S I +AA        ++D +K LL+YN  I+ I  + +  L +AA+ G   + K+LL+  A  +   +   +PL  A    +  V+ LLL   A+I  + +SG + L +++  G+  I+  LL +G      +    TPLH+AS  G +E                                  V LLL+  A PN     G  PL ++ +EG+++    L+D+  +     +  G T L +A      +  E LL RGA+      NG TPLH++V  +  D   +L+         + +    +G TA+HIA+ +   E+  SL+  GA  + +  +G T LHL + +G   I  LLI    N++     G T L + +QEGH+ I ++L+  GA+V        TP  VA    +IK+   L +  A V+ K 
Sbjct:   89 VTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGAN------DELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAA-----QGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKM-TKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKG-----GSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNSKT 669          

HSP 2 Score: 190.274 bits (482), Expect = 2.033e-48
Identity = 154/571 (26.97%), Postives = 262/571 (45.88%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINL-----------IDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLA--RGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGA 3322
            S   + R  + +   + I NG+DIN  N+NG                                  +AL LA   G++++V  LL   I  + T   G TAL  AA  G+  +V  L++ G+N+N Q  +G TPL  A  +    +VK LL NGA       DG + + VA         + +++ LL+ Y     +           +   G   L IAA+  +  + ++LL   A+ ++   +PL  AA +G+F ++ +LL  GA I    K+G S + ++A   +   + +LL     +D++  D+   TPLH+A+  G +    +LL  GAKPN    +G  PL ++C++ ++++ + L+     S+   +  G T L +A        V+ LL +GA  +  +    TPLH++     ++  + LLQ     N   ++    D +T +H A+  G  E++K L++  A  +     G + LH+ A +G  Q  +LL+D      +   +G T L +AS+ G +++  LL+  GAN         TP  VA   N++ + NLL   G 
Sbjct:   10 SFLRAARSGNLDKALEHIKNGIDINTANQNG---------------------------------LNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVAL-----QQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDEL---TPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHL--TPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLK-HSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQ-----NAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGG 531          

HSP 3 Score: 157.532 bits (397), Expect = 2.534e-38
Identity = 129/477 (27.04%), Postives = 217/477 (45.49%), Query Frame = 2
Query: 1922 FTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHC-----------IDKSGFSVLSISASQGNSQILSELLARGL-DEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            ++ + G + LR AA  G  D  L  + +G +IN  +  G   L  A+ +    MV  LL+NG  ++     G +A+ +AAL          ++  L+ Y  N+N    +G   L +AA E    + K LLEN A+  I      +PL  A   G+  VV LL+ +G                +  +GF+ L I+A   N  +   LL RG  DE+       TPLH A+  G +  + +LL +GA       +G  P+ ++ Q  ++   ++L+    E ++  + D  T L +A         + LL +GA    +  NG TPLHI+  ++      +LL+HS +     I  + + G T +H+AS+ G   I+K L+  GA     + +  T LH+ +  G +++ + L+     +D       T L  A++ GH E+  LL+   AN     +   +P  +A +  H++   LL
Sbjct:    3 YSADAGNSFLR-AARSGNLDKALEHIKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQ-----VVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQSIPTEDGFTPLAVALQQGHENVVALLISYGTKGKVRLPALTDFCFFLSVTGFTPLHIAAHYENLNVAQLLLNRGANDEL-------TPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAE-IDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSAS-----IEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLL 460          

HSP 4 Score: 106.301 bits (264), Expect = 1.393e-22
Identity = 78/310 (25.16%), Postives = 143/310 (46.13%), Query Frame = 2
Query: 1589 ILNITSTYSNSIEATNV-YSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLAR 2506
            I+ I      S  A+NV     L  + R   +     L+ N   ++   ++  T L  AA+ G   +  LLL   AN       G S L +A   G+++ V+LLL  +         G T L  A+  G+ D+  LLL  G+N N     G TPL  A +  +  +V LL++ G        +G +A+ +AA         +++   LL++  + N    +G+  L +A+ EG   +  +L+   A++++ ++S   PL   A  G+  +  +L+  GA+++   + G++ L ++   GN +++  LL +
Sbjct:  357 IVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAA-----KQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQ 661          

HSP 5 Score: 64.3142 bits (155), Expect = 7.978e-10
Identity = 43/157 (27.39%), Postives = 73/157 (46.50%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYID 2119
             L  ++  N+ + ++ L+  G   +    NG T+L +AA+     + N LL  GA+       G + L LA   G  ++V LL+S++ + +     G T L   A  G   I  +L+  G+++      G TPL  A +  +  MVK LL   A ++
Sbjct:  510 PLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVN 666          
BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Match: ankrd44 (ankyrin repeat domain 44 [Source:NCBI gene;Acc:101173198])

HSP 1 Score: 208.379 bits (529), Expect = 1.674e-54
Identity = 185/678 (27.29%), Postives = 306/678 (45.13%), Query Frame = 2
Query: 1496 DDIVKILFESINLVNINKLNAKKSFTIFS---------TYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPD-FTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLC-KILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVE---------------------------------GQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRL-----------------------PEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCS-QGATALSIASQEGHLEICNLLINF--GANVCHI-DSCMRTPYKVAYKGNHIKICNLL 3307
            ++I  ++++S    +IN L+A+K   + +         T +L ++    N+ +  N++   L  ++   S + +  LI +  D+N  ++N  T L VAA N       +++    +  + D+ G +AL  A  NG+ E+V LLL++  + + F   DGR AL  AA++G  D+V +L+S G+ ++ +D  G TPL AA       +VK LL+    ID  +  G +A+ +A  C N       ++  L+ Y  N++  +N+G   L  AA      LC + L+ N ADI+I  R   SPL   A  G F   + L+  G  I+ +DK G + L ++A  G+  +++ L+  G D   R   G  PLHLA++                                  G  +CV LLLKSG + N  D  GR PL  +    +    E L+ V    +N     G ++L  A  + L                        + +EFLL  GA A ++D  G +P+H +       C ++LL    TH+         + ++ +H+A++ G  + ++ L+  G  +D +D  GRT L L A +G       L+  G +   T    G T + +A   GH     LL++   GA V  + D   RTP  +A  G H+   +LL
Sbjct:   22 EEIRMLIYKS---EDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKD--NMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGR-ALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLA--CFNGQDA---VVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLV-VCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLD---THDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLL 684          

HSP 2 Score: 193.356 bits (490), Expect = 9.742e-50
Identity = 177/597 (29.65%), Postives = 275/597 (46.06%), Query Frame = 2
Query: 1685 TISQ-LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAA---------LCLNSNSKYLDI---------IKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLE-NNADIDIVD----RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDN-FGCTPLHLASVEGQYECVNLLL--KSGAKPNEI-DNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQ--------------------------------------SQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLID-VGARI-DLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVA 3271
            T SQ LI NG +IN V+++G T L VAA+ G   + N L++SGA+       G   L LA  N + +  + LLS     D   + GRT L  AA  G  D V LLL SG   N+ D  GRTPL  A     Y  ++ L+  G  I+  D  GRSA+  AA         + L  +S+ +           ++ LL+      L D +G + +  AA  G +   ++LL+ ++   +  D    +SPL  AA+ G+ + +++LL  G  +   D++G + L+++A +G++  +  LL++G   +  D  +G TP+HLA + G   CV LLL    GA+  ++ D  GR PLML+   G++     L++    +VN     G  +L +A+L    E V+ LL + A     D+ GRTP+H++  Q                                       Q  C +VLL+           C+D +  T +H A   G       L+D +G+ I   +D +GRT LH  A+ G  +  QLL+     +D     G T L  A+ +G +     L++ GA +  ID    TP  VA
Sbjct:  320 TRSQTLIQNGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRHYHCLETLVVCGTDINATDQWGRSALHYAAASDLDRRRRVALEPDSEGVQAEKEKEAAQCLEFLLQSGAAAALKDKQGYSPVHYAAAFGHRHCLELLLDTHDGRQEEADCGNAQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLE-REANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQ------RGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVA 909          

HSP 3 Score: 184.111 bits (466), Expect = 6.785e-47
Identity = 144/483 (29.81%), Postives = 230/483 (47.62%), Query Frame = 2
Query: 1847 ALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNH 3286
            AL  AI++G  E +++L+ +  D +    + RT L  AA++G  +I  LL+ SG+ +N +D+   TPL  A        V++L+ + A ++ +D + ++ + VAA   N+  +  ++I  LL    ++N+ D  G   L  AA  G   +  +LL   A+I+  D+    A   AAF G+ +VV +L+  GA + C DK G++ L  +AS G   ++  LL+  ++    + FG T LHLA   GQ   V+ L+  GA  ++ +N G  PL  +                       S  G+  L            EFL++ GAD  IQ  +G++PLH++ +  ++   Q L+Q     N   IN +D DG T +H+A+  G   +I +LI  GA    +   G   LHL A       C+ L+  G  ID   S G T L  A+  G+++   LL+  G      D C RTP   A    H
Sbjct:   11 ALIQAIFSGDPEEIRMLIYKSEDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAA--ANNALRCAEVIIPLL---TSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAA---------------------ASTHGALCL------------EFLVNNGADINIQSRDGKSPLHMTAVHGRFTRSQTLIQ-----NGGEINSVDKDGNTPLHVAARYGHELLINTLITSGADCARRGVHGMFPLHLAALNAHSDCCRKLLSSGFQIDTPDSLGRTCLHAAAAGGNVDCVQLLLKSGGEHNRTDKCGRTPLHYAAASRH 450          

HSP 4 Score: 133.65 bits (335), Expect = 3.998e-31
Identity = 136/446 (30.49%), Postives = 194/446 (43.50%), Query Frame = 2
Query: 1736 NGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGN-DGRTALRTAAWIGRTDIVLLLL--SSGSNI-NKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEG-----SKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL-ARGLDEMH-RDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIA-----------VLNRLPEF-------------------------VEFLLSRGADATIQDNNGRTP-LHISVLQSQYDCFQVLLQHSLTHN 2920
            N  + L +AA +G      +LL  G      D+ G + L LA   G+ + V  LLSQ   P  T    GRT +  A   G T  V LLL    G+ + +  D +GRTPLM A        V LLL   A ++  D  G  A+ +A LC        + ++ LL  +  +   D++G   + +AA +G     S+LL     E+++   + D S   PL  A F G    V +LL       CID + F+ L  +   G+    + LL A G D    RD  G TPLH A+  GQ EC+ LLL   A  +  D+HGR PLM +  +G +   E L+D     ++    DG+T L +A           +L RL +                          V+ LLSRGA     D +G TP L  +  +   DC    L HS +H+
Sbjct:  561 NAQSPLHLAAYHGHAQALQVLLRGGREVDERDQAGRTPLALAALRGHADCVHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQD-----ECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQ-RGCRCIDGNPFTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAG-AGLSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQTVDEDGLTPALACAPSRQVADC----LAHSGSHD 995          

HSP 5 Score: 129.028 bits (323), Expect = 1.001e-29
Identity = 88/302 (29.14%), Postives = 145/302 (48.01%), Query Frame = 2
Query: 2327 IDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEIC-NLLINFGANV 3229
            + + D+  L+ A FSG+ E +R+L++    I+ +D    + L  +A  G+++I   L+  G     +DN   TPLH A      + V +L++  A  N  D + + PL ++     ++ AE +I +   SVN     G T+L  A LN   E V  LL++GA+    D      LH +      D   VL+ H        ++C D  G T +H A+  G   ++K L+ +   ID  +  G T LHL  + G   +   LID+G N+ +  ++G T L  A+   H  +C   L+N GA++
Sbjct:    4 LKLADQPALIQAIFSGDPEEIRMLIYKSEDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLL-TSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGA-----EVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNAFGNTALHLACFNGQDAVVSELIDYGANVSQPNNKGFTPLHFAAASTHGALCLEFLVNNGADI 299          

HSP 6 Score: 93.2041 bits (230), Expect = 9.652e-19
Identity = 104/375 (27.73%), Postives = 158/375 (42.13%), Query Frame = 2
Query: 1589 ILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLD----INCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGR----TDIVLLLLSSGSNINK-QDSEGRTPLM-AATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNIN-LIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDE--MHRDNFGC-TPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSC 2662
            +  + S  ++ +    +Y  +       N + T  +L+++  D    ++  +  G T LM+A   G     +LLL   AN    DK G  AL LA+     E V+ LL Q        + GRT +  AA  G     ++++ +  S  S++   +D  G TPL  A  Y  D  +  LL   G        DG    F    C   N        LL     +I    D +G   L  AA+ G     ++LL ++A +D  D   R+PLM AA  G    +  LL  GA +  ID+ G + L ++ S G    +  +L R  D   ++  N    TPLH+A+  G  + V  LL  GA    +D  G  P  L+C
Sbjct:  611 VHTLLSQGASPLSTDQLYGRTPVHLAVMNGHTTCVRLLLDEPDGAEVLDVADPQGRTPLMLAVVGGHVDAVSLLLEREANVNTADKKGLMALHLALLCAQDECVQCLLEQDACLLQGDSQGRTPIHLAAAQGHASWLSELLTIACSESSSVPPLKDGSGYTPLHWACFYGQDGCVEVLLEQRGCRC----IDGNP--FTPLHCAVVNGHESCAALLLDAMGSDITGCRDAKGRTPLHAAAFAGQTECIQLLLSHDAPVDPADDHGRTPLMTAAAKGRVGALEALLDAGAGLSLIDRDGNTPLHVACSNGTEDGVLMILQRLSDATLINATNAAMQTPLHMAAQRGLKKAVQELLSRGASTQTVDEDGLTP-ALAC 978          

HSP 7 Score: 81.6481 bits (200), Expect = 4.226e-15
Identity = 60/211 (28.44%), Postives = 94/211 (44.55%), Query Frame = 2
Query: 2711 ESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            E +N    +  T L  A      E  E L+  GA    +DN   TPLH +V     D  QVL++HS      ++N  D + +T +H+A+        + +I +   +++ D  GRT LH  A  G  ++  LL+  G NI+    +   AL  A+  GHL++ ++L++ GA V   D    TP   A     I +   L      +D  N 
Sbjct:   32 EDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDNMWFTPLHRAVASRSQDAVQVLIRHSA-----DVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMGHLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQIAVVKHLLSLSVEIDEPNA 237          

HSP 8 Score: 60.077 bits (144), Expect = 1.368e-8
Identity = 54/194 (27.84%), Postives = 86/194 (44.33%), Query Frame = 2
Query: 2909 LTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDN---------------------------------EGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSC-MRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*LFYVIDIGFSSYYI 3388
            L +   +IN LD + RT +H A++ GD EI + LI  GAR++ KDN                                   +T LH+ A     +  +++I    +++ +   G TAL  A+  GH E+ NLL+  GAN+   D    R  +  A+ G H+ + ++L  HGA V  K+   +  +    SS  I
Sbjct:   27 LIYKSEDINVLDAEKRTPLHAAAFLGDAEITELLILSGARVNAKDNMWFTPLHRAVASRSQDAVQVLIRHSADVNARDKNWQTPLHVAAANNALRCAEVIIPLLTSVNVSDRGGRTALHHAALNGHTEMVNLLLAKGANINAFDKKDGRALHWAAFMG-HLDVVSVLVSHGAEVSCKDKRGYTPLHAAASSGQI 219          
BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Match: SMESG000028414.1 (SMESG000028414.1)

HSP 1 Score: 2053.87 bits (5320), Expect = 0.000e+0
Identity = 1028/1052 (97.72%), Postives = 1029/1052 (97.81%), Query Frame = 2
Query:  188 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS                       MNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSG+SVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV
Sbjct:   55 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS-----------------------MNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 1083          

HSP 2 Score: 86.2705 bits (212), Expect = 1.058e-16
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 1
Query:   28 MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA 147
            MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA
Sbjct:    1 MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA 40          
BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Match: SMESG000028414.1 (SMESG000028414.1)

HSP 1 Score: 2047.71 bits (5304), Expect = 0.000e+0
Identity = 1028/1055 (97.44%), Postives = 1029/1055 (97.54%), Query Frame = 2
Query:  188 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS                       MNMLLIAAYEGSKLLCKILLENNADIDIVDR   SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSG+SVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV
Sbjct:   55 KSTICRLLTTELTIDCFKTQKLKSLLLASHFCSASDIDSLNLAKFIFEIHQTLLKSKYFGEIYSRNINSSGLCNLFLSQNLCNYPDRAFKNGILLPLLSFPPPNENMYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNFFPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRIDKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIKHIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCALLNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTPKYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHFAVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLNAKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS-----------------------MNMLLIAAYEGSKLLCKILLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 1086          

HSP 2 Score: 86.2705 bits (212), Expect = 1.096e-16
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 1
Query:   28 MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA 147
            MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA
Sbjct:    1 MINFIHGRSVNDSNLISKYLIQREWVFQKILHCINTRSCA 40          
BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Match: SMESG000028414.1 (SMESG000028414.1)

HSP 1 Score: 935.636 bits (2417), Expect = 0.000e+0
Identity = 463/490 (94.49%), Postives = 464/490 (94.69%), Query Frame = 2
Query: 1883 VVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            VVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS                       MNMLLIAAYEGSKLLCKILLENNADIDIVDR   SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSG+SVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV
Sbjct:   66 VVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS-----------------------MNMLLIAAYEGSKLLCKILLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 532          

HSP 2 Score: 117.087 bits (292), Expect = 6.963e-27
Identity = 102/339 (30.09%), Postives = 172/339 (50.74%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV--KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLE-----NNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKI 2683
            L+ N  DI+ V+ N  + LM AA +G   +  LLL  GA    IDK G+S L ++   G  +++  LL++ +D     N G T L  A+  G+ + V LLL SG+  N+ D+ GR PLM +  +  Y+ +  KL+      ++ +  DG +++ +A L     ++  + ++ LL    +  + DN G   L I+  +      ++LL+     N  +I+ +D   R+ +  A++ G+ E+++ L+  GA I   D  G + L + A QG  QI   L+  G++     + G T L +AS EG  E  NLL+  GA    ID+  R P  ++ +  +IKI
Sbjct:  184 LLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQE-GYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVL-----NRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKI 516          

HSP 3 Score: 112.849 bits (281), Expect = 1.746e-25
Identity = 107/395 (27.09%), Postives = 184/395 (46.58%), Query Frame = 2
Query: 1589 ILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLIINGLDINCVNENGTTS---------------LMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDII-KLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAA-----IHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLID 2701
            +L + S+ SN  +  +     L  +  +  Y  +  L+ NG  I+  + +G ++               L++AA  G   +C +LL + A+  ++D++  S L  A ++G  EVV+LLL            G + L  +A  G + I+  LL+ G +   +D+ G TPL  A+ +  Y  V LLL +GA  +  DN GR       L L+    Y+ I  KL+  +  ++N    +G   L IA         + LL   AD  I D   R+PL  +     ++  ++LL          I+C+D  G + + I++ +G+ +I+  L+  G     +DN G T LHL + +G Y+   LL+  G   +E  + G   L ++ QEG+++I   LI+
Sbjct:  100 VLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSMNMLLIAAYEGSKLLCKILLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGR-----IPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLIN 489          

HSP 4 Score: 104.375 bits (259), Expect = 9.498e-23
Identity = 86/266 (32.33%), Postives = 137/266 (51.50%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDP-DFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPL----MAATYKCDYVMVK-LLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIV---DRSPLMAAAFSGNFEVVRLLLFWGAAIHCID 2431
            S  Q +   +S+L+  GLD    +  G T L +A+  G     NLLL SGA    ID  G   L L+   GYI++ + L+    +  +F   DG T+L+ A      + V  LLS G++   QD+ GRTPL    + + Y C  V+++  L +N   I+  D+DGR+AI +A+   +      +IIK L+     I+L DNEG   L + A++G   +C++L++   +ID       + L  A+  G+ E+  LL+ +GA +  ID
Sbjct:  238 SASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDG-----EIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHID 498          

HSP 5 Score: 73.9442 bits (180), Expect = 3.946e-13
Identity = 51/162 (31.48%), Postives = 89/162 (54.94%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGL-----DINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQD 2128
            S+ Q+ Y+    L+ + L     +INC++++G T++ +A+  G   I   L+  GA   + D +G + L L  W G  ++ +LL+   I+ D T + G TAL  A+  G  +I  LL++ G+N+   DS  RTP   A YK +++ +  LL  +GA +D ++
Sbjct:  371 SVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVA-YKGNHIKICNLLEKHGALVDRKN 531          
BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Match: SMESG000028414.1 (SMESG000028414.1)

HSP 1 Score: 934.095 bits (2413), Expect = 0.000e+0
Identity = 463/491 (94.30%), Postives = 465/491 (94.70%), Query Frame = 2
Query: 1880 EVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 3343
            +VVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS                       MNMLLIAAYEGSKLLCKILLENNADIDIVDR   SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSG+SVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV
Sbjct:   71 KVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNS-----------------------MNMLLIAAYEGSKLLCKILLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV 538          

HSP 2 Score: 117.087 bits (292), Expect = 7.274e-27
Identity = 102/339 (30.09%), Postives = 172/339 (50.74%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV--KLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLE-----NNADIDIVD---RSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKI 2683
            L+ N  DI+ V+ N  + LM AA +G   +  LLL  GA    IDK G+S L ++   G  +++  LL++ +D     N G T L  A+  G+ + V LLL SG+  N+ D+ GR PLM +  +  Y+ +  KL+      ++ +  DG +++ +A L     ++  + ++ LL    +  + DN G   L I+  +      ++LL+     N  +I+ +D   R+ +  A++ G+ E+++ L+  GA I   D  G + L + A QG  QI   L+  G++     + G T L +AS EG  E  NLL+  GA    ID+  R P  ++ +  +IKI
Sbjct:  190 LLENNADIDIVDRNFRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGYSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQE-GYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVL-----NRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKI 522          

HSP 3 Score: 73.9442 bits (180), Expect = 4.183e-13
Identity = 51/162 (31.48%), Postives = 89/162 (54.94%), Query Frame = 2
Query: 1661 SIRQNSYNTISQLIINGL-----DINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMV-KLLLNNGAYIDHQD 2128
            S+ Q+ Y+    L+ + L     +INC++++G T++ +A+  G   I   L+  GA   + D +G + L L  W G  ++ +LL+   I+ D T + G TAL  A+  G  +I  LL++ G+N+   DS  RTP   A YK +++ +  LL  +GA +D ++
Sbjct:  377 SVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVA-YKGNHIKICNLLEKHGALVDRKN 537          
BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Match: SMESG000026655.1 (SMESG000026655.1)

HSP 1 Score: 213.772 bits (543), Expect = 6.898e-56
Identity = 161/544 (29.60%), Postives = 272/544 (50.00%), Query Frame = 2
Query: 1697 LIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKI-LLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECV-NLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEK 3313
            LI    ++N V  +G + +  AA++GF +I  +LL +GA+     K+G +AL++A      EV + L+++ ID D    D  TAL  AA  G  +   +LL +  N+N +   G TPL  A  K    MV+LL+   A+I+     G + + +AA      SK  +I+ +LL+   +I+    +  N L +AA    +   KI LL   A+ +   R   +PL  A   GN+ +V  LL  GA  + + K  ++ L I+A +G  +I   LL        +   G TPLH+AS  G+ +    LLL +   PN + +HG  PL ++    + ++ E L+D    +VN    +G T L +     + E  + L+ RGA+   Q   G TPLH++ +    +  ++LL  +      ++N   ++G TA+H+A+ K   +I++ L+   A I  K   G T LH     G  Q  ++L++   +++   + G T+L +A+Q+GH+ I  LL++   +V H +    T  +VA K +++ I   L K
Sbjct:  259 LISQKANVNSVTRDGLSPIHCAARSGFENIILILLENGADIQHKSKNGLTALQMATQGDQAEVCQTLINKGIDVDAVTMDYLTALHVAAHCGNINAAEVLLKNNCNVNARALNGFTPLHIACKKIQSKMVELLIKYKAHINSTTETGLTPLHIAAFM---GSK--EIVNILLKNGASISQTTMKCENALHLAARNNQEETFKILLLHPEANFNARARENQTPLHIAVRMGNYSIVEQLLNAGADPNMVTKDNYTALHIAAKEGRHEISRLLLEHQCKPDAKTKKGFTPLHIASKYGRVQVARQLLLSAKVNPNSLGHHGLTPLHVATYYNHYEMIELLLDTEA-NVNCLYKNGYTPLHLVAKKNMIEVTKLLIDRGANVNAQSQKGFTPLHLNSMAGNKEIMELLLDANA-----DVNHQAENGLTALHLAAEKNRTDIVEQLLKTEADISTKTQMGYTALHTAVHFGQIQTVKMLLEKNFDVNAQTNLGCTSLHLAAQQGHIAIVRLLLDNEIDVNHQNKQGLTALEVAQKQHYLNIAETLSK 791          

HSP 2 Score: 187.193 bits (474), Expect = 1.562e-47
Identity = 154/589 (26.15%), Postives = 270/589 (45.84%), Query Frame = 2
Query: 1643 SFSLFESIRQNSYNTISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAY------------------------------IDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDR---SPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEIC-NLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLL 3307
            + +   + R  +   + + +   +  +  N NG  ++ +AA+ G   +  LL+ +G +  +  + G ++L +A   G +EVV LLL  K + +    +G T L  AA     D+V +LL  G+N      +G TPL  A  + +  +V++LL+N +                               +DHQ   G + + +AA   N N     + KLL+  N ++N      +  L +AA  G + + K+L+   A+++ V R   SP+  AA SG   ++ +LL  GA I    K+G + L ++     +++   L+ +G+D         T LH+A+  G      +LLK+    N    +G  PL ++C++   K+ E LI  +   +N  +  G T L IA      E V  LL  GA  +         LH++   +Q + F++LL H       N N    + +T +HIA   G+  I++ L++ GA  ++   +  T LH+ A +G ++I +LL++     D    +G T L IAS+ G +++   LL++   N   +     TP  VA   NH ++  LL
Sbjct:   13 AHAFLRAARSGNVEKVKEFLQADIKPDMSNANGLNAMHLAAKEGNREVVQLLIENGLSVDIATRKGNTSLHIASLAGKLEVVSLLLESKANINVKSQNGFTPLYMAAQENHLDVVKVLLEYGANQAITTDDGFTPLAVALQQGNDKVVQILLDNDSRSKVRLPALHIAAKKDDVNAASLLIGSSDDNVDHQSASGFTPLHIAAHYNNVN-----VAKLLIEKNADVNFPAKNNITPLHVAAKWGKEEMLKLLISQKANVNSVTRDGLSPIHCAARSGFENIILILLENGADIQHKSKNGLTALQMATQGDQAEVCQTLINKGIDVDAVTMDYLTALHVAAHCGNINAAEVLLKNNCNVNARALNGFTPLHIACKKIQSKMVELLIK-YKAHINSTTETGLTPLHIAAFMGSKEIVNILLKNGASISQTTMKCENALHLAARNNQEETFKILLLHPEA----NFNARARENQTPLHIAVRMGNYSIVEQLLNAGADPNMVTKDNYTALHIAAKEGRHEISRLLLEHQCKPDAKTKKGFTPLHIASKYGRVQVARQLLLSAKVNPNSLGHHGLTPLHVATYYNHYEMIELL 591          

HSP 3 Score: 177.563 bits (449), Expect = 1.243e-44
Identity = 163/559 (29.16%), Postives = 262/559 (46.87%), Query Frame = 2
Query: 1688 ISQLIINGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAW--IGRTDIV----LLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPE-FVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQIC-QLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVD 3331
            +S L+ +  +IN  ++NG T L +AAQ     +  +LL  GANQ +   DG++ L +A+  G  +VV++LL          ND R+ +R  A     + D V    LL+ SS  N++ Q + G TPL  A +  +  + KLL+   A ++    +  + + VAA          +++KLL+    N+N +  +G++ +  AA  G + +  ILLEN ADI    ++ L A   A      EV + L+  G  +  +     + L ++A  GN      LL    +   R   G TPLH+A  + Q + V LL+K  A  N     G  PL ++   G  +I   L+     S++  +     +L +A  N   E F   LL   A+   +    +TPLHI+V    Y   + LL         + N +  D  TA+HIA+ +G  EI + L++   + D K  +G T LH+ +  G  Q+  QLL+   +N +     G T L +A+   H E+  LL++  ANV  +     TP  +  K N I++  LL   GA V+
Sbjct:   94 VSLLLESKANINVKSQNGFTPLYMAAQENHLDVVKVLLEYGANQAITTDDGFTPLAVALQQGNDKVVQILLD---------NDSRSKVRLPALHIAAKKDDVNAASLLIGSSDDNVDHQSASGFTPLHIAAHYNNVNVAKLLIEKNADVNFPAKNNITPLHVAAKWGKE-----EMLKLLISQKANVNSVTRDGLSPIHCAARSGFENIILILLENGADIQHKSKNGLTALQMATQGDQAEVCQTLINKGIDVDAVTMDYLTALHVAAHCGNINAAEVLLKNNCNVNARALNGFTPLHIACKKIQSKMVELLIKYKAHINSTTETGLTPLHIAAFMGSKEIVNILLKNGA-SISQTTMKCENALHLAARNNQEETFKILLLHPEANFNARARENQTPLHIAVRMGNYSIVEQLLNAGA-----DPNMVTKDNYTALHIAAKEGRHEISRLLLEHQCKPDAKTKKGFTPLHIASKYGRVQVARQLLLSAKVNPNSLGHHGLTPLHVATYYNHYEMIELLLDTEANVNCLYKNGYTPLHLVAKKNMIEVTKLLIDRGANVN 632          

HSP 4 Score: 172.17 bits (435), Expect = 6.129e-43
Identity = 163/570 (28.60%), Postives = 257/570 (45.09%), Query Frame = 2
Query: 1649 SLFESIRQNSYNTISQLIINGLD-INCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRS---PLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELL---ARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLKIAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQHSLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGRTGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNLLINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRK 3337
            +L  + +++  N  S LI +  D ++  + +G T L +AA     ++  LL+   A+     K+  + L +A   G  E++KLL+SQK + +    DG + +  AA  G  +I+L+LL +G++I  +   G T L  AT      + + L+N G  +D    D  +A+ VAA C N N+      ++LL+ NCN+N     G   L IA  +    + ++L++  A I+    +   PL  AAF G+ E+V +LL  GA+I        + L ++A     +    LL       +   R+N   TPLH+A   G Y  V  LL +GA P                                  N  + D  T+L IA      E    LL        +   G TPLHI+   S+Y   QV  Q  L+  +N  N L   G T +H+A++    E+I+ L+D  A ++     G T LHL A +   ++ +LLID G N++    +G T L + S  G+ EI  LL++  A+V H      T   +A + N   I   L K  A +  K
Sbjct:  176 ALHIAAKKDDVNAASLLIGSSDDNVDHQSASGFTPLHIAAHYNNVNVAKLLIEKNADVNFPAKNNITPLHVAAKWGKEEMLKLLISQKANVNSVTRDGLSPIHCAARSGFENIILILLENGADIQHKSKNGLTALQMATQGDQAEVCQTLINKGIDVDAVTMDYLTALHVAAHCGNINAA-----EVLLKNNCNVNARALNGFTPLHIACKKIQSKMVELLIKYKAHINSTTETGLTPLHIAAFMGSKEIVNILLKNGASISQTTMKCENALHLAARNNQEETFKILLLHPEANFNARAREN--QTPLHIAVRMGNYSIVEQLLNAGADP----------------------------------NMVTKDNYTALHIAAKEGRHEISRLLLEHQCKPDAKTKKGFTPLHIA---SKYGRVQVARQLLLSAKVNP-NSLGHHGLTPLHVATYYNHYEMIELLLDTEANVNCLYKNGYTPLHLVAKKNMIEVTKLLIDRGANVNAQSQKGFTPLHLNSMAGNKEIMELLLDANADVNHQAENGLTALHLAAEKNRTDIVEQLLKTEADISTK 700          

HSP 5 Score: 143.665 bits (361), Expect = 3.657e-34
Identity = 115/381 (30.18%), Postives = 181/381 (47.51%), Query Frame = 2
Query: 1580 STYILNITSTYSNSIEATNVY-SFSLFESIRQNSYNTISQLIING-LDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALRLAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVL-LLLSSGSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFVAALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKILLENNADIDIVDRSPLMA---AAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLSISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKSGAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDV 2704
            S  I+NI      SI  T +    +L  + R N   T   L+++   + N       T L +A + G  SI   LL++GA+  M+ KD ++AL +A   G  E+ +LLL  +  PD     G T L  A+  GR  +   LLLS+  N N     G TPL  ATY   Y M++LLL+  A ++    +G + + + A         +++ KLL+    N+N    +G   L + +  G+K + ++LL+ NAD++    + L A   AA     ++V  LL   A I    + G++ L  +   G  Q +  LL +  D   + N GCT LHLA+ +G    V LLL +    N  +  G   L ++ ++ Y+ IAE L  V
Sbjct:  417 SKEIVNILLKNGASISQTTMKCENALHLAARNNQEETFKILLLHPEANFNARARENQTPLHIAVRMGNYSIVEQLLNAGADPNMVTKDNYTALHIAAKEGRHEISRLLLEHQCKPDAKTKKGFTPLHIASKYGRVQVARQLLLSAKVNPNSLGHHGLTPLHVATYYNHYEMIELLLDTEANVNCLYKNGYTPLHLVA-----KKNMIEVTKLLIDRGANVNAQSQKGFTPLHLNSMAGNKEIMELLLDANADVNHQAENGLTALHLAAEKNRTDIVEQLLKTEADISTKTQMGYTALHTAVHFGQIQTVKMLLEKNFDVNAQTNLGCTSLHLAAQQGHIAIVRLLLDNEIDVNHQNKQGLTALEVAQKQHYLNIAETLSKV 792          
The following BLAST results are available for this feature:
BLAST of Ankyrin repeat domain 50 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD504.485e-6837.86ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
ANKRD501.892e-6838.42ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
ANK32.162e-6028.67ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK34.504e-6029.25ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK32.987e-5929.25ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
T28D6.43.305e-6132.01pep chromosome:WBcel235:III:11360456:11366214:1 ge... [more]
unc-443.019e-5026.96AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-443.019e-5026.96AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-446.795e-5027.12AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-446.795e-5027.12AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG100112.633e-12943.22gene:FBgn0039590 transcript:FBtr0085319[more]
Ank21.464e-5729.26gene:FBgn0261788 transcript:FBtr0303119[more]
Ank24.734e-5729.75gene:FBgn0261788 transcript:FBtr0334061[more]
Ank21.410e-5629.26gene:FBgn0261788 transcript:FBtr0303117[more]
Ank21.413e-5629.26gene:FBgn0261788 transcript:FBtr0303121[more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD507.538e-17637.71ankyrin repeat domain 50 [Source:HGNC Symbol;Acc:H... [more]
ankrd50l5.654e-11539.22ankyrin repeat domain 50-like [Source:ZFIN;Acc:ZDB... [more]
ank3a1.066e-6028.45ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank3a1.066e-6028.45ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
BX469921.11.079e-6028.90ankyrin 3a [Source:NCBI gene;Acc:103909035][more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gart6.001e-6738.03phosphoribosylglycinamide formyltransferase, phosp... [more]
tpm42.965e-5929.85tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097][more]
tpm43.068e-5929.85tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097][more]
tpm43.908e-5929.88tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097][more]
tpm44.115e-5929.88tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097][more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ankrd501.102e-6637.76ankyrin repeat domain 50 [Source:MGI Symbol;Acc:MG... [more]
Ank39.422e-6128.67ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank31.032e-6029.25ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank32.221e-6029.25ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
Ank32.351e-6029.25ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:8... [more]
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BLAST of Ankyrin repeat domain 50 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9ULJ7|ANR50_HUMAN2.154e-6737.86Ankyrin repeat domain-containing protein 50 OS=Hom... [more]
sp|G5E8K5|ANK3_MOUSE1.645e-5929.25Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV... [more]
sp|Q12955|ANK3_HUMAN1.435e-5829.25Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=... [more]
sp|O70511|ANK3_RAT6.284e-5728.65Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE... [more]
sp|Q5F478|ANR44_CHICK1.317e-5528.55Serine/threonine-protein phosphatase 6 regulatory ... [more]
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BLAST of Ankyrin repeat domain 50 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3H8J00.000e+038.76ankyrin repeat domain-containing protein 50 OS=Lin... [more]
A0A087T2Z36.160e-4538.68Ankyrin repeat domain-containing protein 50 (Fragm... [more]
L7MGU25.481e-3436.34ANK_REP_REGION domain-containing protein (Fragment... [more]
A0A131YW746.447e-3436.30Ankyrin repeat domain-containing protein 50 OS=Rhi... [more]
A0A1S3FLW47.403e-6437.93ankyrin repeat domain-containing protein 50 OS=Dip... [more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD504.797e-6537.38ankyrin repeat domain 50 [Source:NCBI gene;Acc:103... [more]
ankrd50l1.772e-3633.64ankyrin repeat domain-containing protein 50-like [... [more]
ank3b5.293e-6130.09ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
ank3a1.278e-5929.60ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
ank3a1.989e-5729.17ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1][more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000095.11.822e-6731.02pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000000093.14.124e-6530.76pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000007446.11.989e-5028.65pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000008129.11.610e-4525.24pep scaffold:Pmarinus_7.0:GL476358:593185:649018:1... [more]
ENSPMAT00000003991.17.019e-4427.02pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1... [more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
AKR11.448e-1526.14Palmitoyl transferase involved in protein palmitoy... [more]
NAS67.235e-1328.29Evolutionarily conserved 19S regulatory particle a... [more]
AKR23.588e-1028.43Ankyrin repeat-containing protein; member of a fam... [more]
HOS44.185e-1039.00Subunit of the Set3 complex; complex is a meiotic-... [more]
AVO27.025e-828.19Component of a complex containing the Tor2p kinase... [more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO427803.156e-2337.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494851.333e-1930.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO375202.518e-1821.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387022.679e-1723.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348634.171e-1733.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ankyrin repeat domain 50 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankrd50l3.837e-3034.08ankyrin repeat domain 50 [Source:NCBI gene;Acc:101... [more]
ENSORLT00000031565.13.247e-5628.01ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ENSORLT00000039458.14.634e-5628.01ankyrin 2 [Source:NCBI gene;Acc:101161458][more]
ank1a4.721e-5628.26ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ankrd441.674e-5427.29ankyrin repeat domain 44 [Source:NCBI gene;Acc:101... [more]
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BLAST of Ankyrin repeat domain 50 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028414.11.058e-1697.72SMESG000028414.1[more]
SMESG000028414.11.096e-1697.44SMESG000028414.1[more]
SMESG000028414.16.963e-2794.49SMESG000028414.1[more]
SMESG000028414.17.274e-2794.30SMESG000028414.1[more]
SMESG000026655.16.898e-5629.60SMESG000026655.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005209 ID=SMED30005209|Name=Ankyrin repeat domain 50|organism=Schmidtea mediterranea sexual|type=transcript|length=3629bp
ATCTATATAAATCTTATATGGTGTTTGATGATAAATTTTATCCATGGCAG
ATCTGTCAATGATTCTAATTTAATTTCAAAATATTTAATCCAACGAGAGT
GGGTATTTCAAAAAATTTTACATTGTATAAATACTAGAAGCTGCGCAATC
TTGTGGATTGTTGATATTAGGAGGATCTGGCAGTGGTAAAAGCACGATCT
GTCGTCTTCTTACAACTGAACTAACGATTGATTGTTTTAAAACTCAGAAG
TTAAAATCATTGCTTTTAGCCTCTCATTTTTGTTCAGCCAGTGATATTGA
TAGTTTGAATTTAGCTAAATTTATTTTTGAAATCCATCAGACATTATTGA
AATCAAAATATTTTGGTGAAATTTATTCAAGAAATATAAATTCATCTGGG
TTATGTAATTTATTCTTATCTCAAAATCTTTGTAATTATCCTGATAGAGC
TTTTAAAAATGGAATTCTTTTACCATTATTATCATTTCCACCACCCAATG
AAAATATGTATATTTTAATCGATTCTATTGATGAAAACCTCTTATCAGAT
TATTTGGGCGAAAAATTATCATACATTTTTAATAAAGACACCTTTCATAA
AAACAGACAAGGGAGTAAAAATATATCAGATTTGTTATCTGCGCATCACA
ATTTTTTTCCTGAATGGCTATCTCTAGTTTGCACTTGTCGAAAAGAAAAT
GAATTTATTGTTCGTAAATTTATGGGATTTCGTAAATTTTATCTGGATGA
TTTAAATAAAAGCTATGTAATTCGTGATATTCAATTATATATATTGAATA
GAATTGATAAAGATGATAATCTCAAACAAATGATCAATTCAGAAACTACT
GAGTTATTAAATTTATTACACATTAAGAGCAATGGATATATAATTTATTT
GGAAATCGTTCTTGACGGTATACTTGACGGTTATCTTAGTATTCAAGTTA
TTAAGCACATACCCGGAATATTGTGTGGATTATATTTATGGATATTTGAA
ACTCTTTTTAAACATTATCAAGATTTTATAGTTGTGAAAAATATTTTCAA
CATTATGTTATCATCACTTAAATCATTATCAGACCAAGAAATATATTGTG
CTTTGCTAAATTTAGATATAAACACTGATTTGTATTATTTAAAAGATATG
CTCTTCAAAATTAAATATTTTTTAGTTAAAACTTGTGACAATTGCTACCA
TTTTAGGCACAACAGTCTTCCTGAATGGTTGTTGGATGTCAAACTTTGTA
CTCCAAAGTATAAATGTTCTTTAATTGATGGCCATGTTAATTTGGCCCTG
TATAATGCGTATATAAGCTCTAAGCTTGCACCCTTACAGATAAGAAATTT
CGCTTTTCATTTAATTGCATCAGCTCCCATTTTTAAATTCAAAGGTTATC
ATTTTGCTGTTTGGCTTTTATATAGTGATTGTGATATTGAAGTTAAAGGT
ATTTTACCATGTGAAAAATTTGGAAGTCTAAATGACTGGAGCATAGATGA
CATAGTTAAAATACTATTCGAATCTATAAACTTGGTAAATATTAATAAGT
TAAATGCAAAAAAGTCTTTTACAATATTTTCAACATACATTTTAAACATA
ACTTCAACCTATTCTAATTCAATTGAAGCTACAAATGTTTATTCATTTTC
ACTATTTGAATCTATTCGGCAAAATAGTTACAATACAATTTCACAATTAA
TAATTAATGGATTGGATATAAATTGTGTAAATGAAAATGGAACAACTTCG
TTAATGGTTGCTGCACAAAACGGATTTACCAGCATCTGTAATTTGCTTTT
ATCTTCTGGTGCTAATCAATTGATGATTGATAAAGACGGTTGGTCAGCAT
TAAGATTGGCTATTTGGAATGGGTATATTGAAGTAGTTAAACTTCTATTA
TCTCAAAAAATTGATCCTGATTTTACTGGAAACGATGGAAGAACAGCGCT
CAGAACTGCAGCTTGGATCGGAAGGACCGATATTGTTTTGCTTTTACTAT
CTTCTGGTTCAAATATAAACAAACAAGATTCCGAAGGTCGCACTCCTTTA
ATGGCTGCTACTTACAAATGTGATTATGTGATGGTTAAACTATTATTAAA
TAACGGAGCGTATATTGATCATCAAGATAATGATGGACGGAGTGCAATAT
TTGTAGCTGCATTATGCTTGAATTCAAATAGTAAATATCTAGATATTATT
AAATTACTTTTAAGATATAATTGTAATATAAATTTGATTGATAATGAAGG
CATGAACATGCTACTAATTGCAGCTTATGAAGGTAGTAAATTGTTATGCA
AAATACTTTTGGAAAACAACGCAGACATTGATATAGTAGACAGATCACCT
TTGATGGCTGCTGCTTTTTCAGGAAACTTTGAAGTTGTTAGACTATTGTT
ATTTTGGGGAGCTGCTATACATTGTATAGATAAATCCGGGTTTTCTGTAT
TAAGTATATCGGCAAGTCAAGGAAATAGTCAAATATTATCTGAATTATTA
GCCAGAGGCTTAGATGAAATGCATCGTGATAATTTTGGATGTACACCTCT
ACATTTAGCTTCAGTTGAAGGTCAATATGAATGTGTAAATTTATTATTGA
AATCTGGTGCTAAGCCAAATGAAATCGATAATCATGGGCGTATTCCTCTA
ATGCTTTCTTGTCAAGAAGGATATATTAAAATTGCTGAGAAGCTTATTGA
TGTTTGGCCAGAAAGTGTTAATTTTAAGTCTTATGATGGAAGTACTTCTT
TAAAAATAGCGGTCTTGAACAGATTGCCAGAATTTGTGGAATTTTTACTA
AGTCGAGGAGCAGATGCAACTATTCAAGATAATAATGGTCGTACTCCTTT
GCATATATCAGTACTTCAAAGCCAGTATGATTGCTTTCAGGTTTTATTGC
AGCATAGTCTAACTCATAATATAAATAATATCAATTGTCTTGATGACGAT
GGACGAACAGCTATTCATATAGCCTCATGGAAAGGAGATGGGGAAATTAT
TAAGTCATTAATTGATGTTGGGGCAAGAATTGACTTAAAAGATAATGAAG
GACGAACTGGATTGCATTTAGGTGCATGGCAAGGGTGTTATCAAATTTGC
CAATTACTAATTGATTTCGGAATAAATATTGACGAAACTTGCTCTCAAGG
AGCAACAGCATTGAGTATCGCATCTCAAGAAGGGCACTTAGAAATTTGTA
ACTTATTAATTAATTTTGGAGCAAATGTATGTCACATTGATTCATGTATG
CGAACTCCATATAAAGTTGCATACAAAGGAAATCATATTAAAATTTGCAA
CCTACTCGAAAAACATGGAGCTCTTGTGGATAGAAAAAATGTTTGATTAT
TTTACGTTATCGATATAGGTTTTTCAAGTTACTACATCCTGAATTCTTTT
TTTTAATATTCAACTTTTTGAATGTTTAATGGAATTTTGTACATTTAATC
GTGGTGATAGATCTATTTTCCCAAGATTTTTTAAGTATAAATATGGTGTT
ATATATGTGTTTACATTACGTTTGTGAAAGAAAAAATCAGGGCTCAGCCG
AGTCACTTGTAAAACAAAAAACTGTGTCGGGACAGCTGGGATGATTGTAT
ATATTTTTTCTATTGCTCAGATCGGAAGA
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protein sequence of SMED30005209-orf-1

>SMED30005209-orf-1 ID=SMED30005209-orf-1|Name=SMED30005209-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=947bp
MYILIDSIDENLLSDYLGEKLSYIFNKDTFHKNRQGSKNISDLLSAHHNF
FPEWLSLVCTCRKENEFIVRKFMGFRKFYLDDLNKSYVIRDIQLYILNRI
DKDDNLKQMINSETTELLNLLHIKSNGYIIYLEIVLDGILDGYLSIQVIK
HIPGILCGLYLWIFETLFKHYQDFIVVKNIFNIMLSSLKSLSDQEIYCAL
LNLDINTDLYYLKDMLFKIKYFLVKTCDNCYHFRHNSLPEWLLDVKLCTP
KYKCSLIDGHVNLALYNAYISSKLAPLQIRNFAFHLIASAPIFKFKGYHF
AVWLLYSDCDIEVKGILPCEKFGSLNDWSIDDIVKILFESINLVNINKLN
AKKSFTIFSTYILNITSTYSNSIEATNVYSFSLFESIRQNSYNTISQLII
NGLDINCVNENGTTSLMVAAQNGFTSICNLLLSSGANQLMIDKDGWSALR
LAIWNGYIEVVKLLLSQKIDPDFTGNDGRTALRTAAWIGRTDIVLLLLSS
GSNINKQDSEGRTPLMAATYKCDYVMVKLLLNNGAYIDHQDNDGRSAIFV
AALCLNSNSKYLDIIKLLLRYNCNINLIDNEGMNMLLIAAYEGSKLLCKI
LLENNADIDIVDRSPLMAAAFSGNFEVVRLLLFWGAAIHCIDKSGFSVLS
ISASQGNSQILSELLARGLDEMHRDNFGCTPLHLASVEGQYECVNLLLKS
GAKPNEIDNHGRIPLMLSCQEGYIKIAEKLIDVWPESVNFKSYDGSTSLK
IAVLNRLPEFVEFLLSRGADATIQDNNGRTPLHISVLQSQYDCFQVLLQH
SLTHNINNINCLDDDGRTAIHIASWKGDGEIIKSLIDVGARIDLKDNEGR
TGLHLGAWQGCYQICQLLIDFGINIDETCSQGATALSIASQEGHLEICNL
LINFGANVCHIDSCMRTPYKVAYKGNHIKICNLLEKHGALVDRKNV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
IPR027417P-loop_NTPase
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005768endosome
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006886intracellular protein transport
GO:0015031protein transport
GO:1990126retrograde transport, endosome to plasma membrane