notchless-1

Overview
Namenotchless-1 (AFJ24805.1)
Smed IDSMED30005194
Length (bp)1838
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of notchless-1 (SMED30005194) t-SNE clustered cells

Violin plots show distribution of expression levels for notchless-1 (SMED30005194) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of notchless-1 (SMED30005194) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for notchless-1 (SMED30005194) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005194

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005194SMESG000000678.1 SmedASXL_010302SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30005194SMESG000000678.1 dd_Smed_v4_10113_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005194SMESG000000678.1 Contig48534newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005194SMESG000000678.1 Contig48534uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of notchless-1 vs. Ensembl Human
Match: NLE1 (notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:19889])

HSP 1 Score: 419.083 bits (1076), Expect = 1.273e-140
Identity = 227/546 (41.58%), Postives = 313/546 (57.33%), Query Frame = 3 
Query:   66 ESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            E++   V+ + VQF D  G   G  F +P       L    N +L +E P     +FF+ +              IV+++  ++                  S+    E VL I+Y PQ++FRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+G+ LAS    G+I+ W    GK   +T A         K +  L+W+PLH +  CR +A++  D ++ IWDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    8 EAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLP--LAFFVHDA------------EIVSSLGKTL-----------------ESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKG-HRHWVLSISWSPDGRKLASGCKNGQILLWDPSTGKQVGRTLA------GHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 483

HSP 2 Score: 51.9878 bits (123), Expect = 2.618e-6
Identity = 40/153 (26.14%), Postives = 68/153 (44.44%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W              H++ +  + +SP+ +++AS+S    I  W    GK+     A ++ + +A   V  +AW         RLL +   D T+ +WD    +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:  344 LVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYL----ASLRGHVAA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 484
[Close]
BLAST of notchless-1 vs. Ensembl Human
Match: NLE1 (notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:19889])

HSP 1 Score: 348.591 bits (893), Expect = 8.724e-114
Identity = 203/546 (37.18%), Postives = 282/546 (51.65%), Query Frame = 3 
Query:   66 ESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            E++   V+ + VQF D  G   G  F +P       L    N +L +E P     +FF+ +              IV+++  ++                  S+    E VL I+Y PQ++FRVR +TRCTSS+ GH +AV                                           + +++SP GK LAS S    I+ W    GK   +T A         K +  L+W+PLH +  CR +A++  D ++ IWDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    8 EAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLP--LAFFVHDA------------EIVSSLGKTL-----------------ESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAV-------------------------------------------ISVAFSPTGKYLASGSGDTTILLWDPSTGKQVGRTLA------GHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 441

HSP 2 Score: 52.373 bits (124), Expect = 1.759e-6
Identity = 40/153 (26.14%), Postives = 68/153 (44.44%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W              H++ +  + +SP+ +++AS+S    I  W    GK+     A ++ + +A   V  +AW         RLL +   D T+ +WD    +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:  302 LVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKY----LASLRGHVAA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 442
[Close]
BLAST of notchless-1 vs. Ensembl Human
Match: NLE1 (notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:19889])

HSP 1 Score: 257.299 bits (656), Expect = 2.073e-80
Identity = 139/330 (42.12%), Postives = 183/330 (55.45%), Query Frame = 3 
Query:  831 DKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGK---------------------------------------FLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            D ++ IWDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK                                       +L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    5 DGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKDDFHSVTWCSSGSSAAARSWLITTSASWVQVILLPQPPRYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 302
[Close]
BLAST of notchless-1 vs. Ensembl Human
Match: NLE1 (notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:19889])

HSP 1 Score: 195.667 bits (496), Expect = 2.368e-58
Identity = 106/223 (47.53%), Postives = 134/223 (60.09%), Query Frame = 3 
Query: 1035 ISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            ++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    1 MALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 191

HSP 2 Score: 54.299 bits (129), Expect = 9.839e-8
Identity = 40/153 (26.14%), Postives = 68/153 (44.44%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W              H++ +  + +SP+ +++AS+S    I  W    GK+     A ++ + +A   V  +AW         RLL +   D T+ +WD    +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:   52 LVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKY----LASLRGHVAA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDVKAQKLAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 192

HSP 3 Score: 51.2174 bits (121), Expect = 1.045e-6
Identity = 38/138 (27.54%), Postives = 62/138 (44.93%), Query Frame = 3 
Query:  438 RPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIW 851
            +P+TR    M GH+  +    FSPD R +A+ S D +++ WD  T    A +   H + V  ++WS + ++L S S    +  W +       K   L          V ++ W P   DG  + +A+   DK + IW
Sbjct:   71 KPLTR----MTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLR-GHVAAVYQIAWSADSRLLVSGSSDSTLKVWDV-------KAQKLAMDLPGHADEVYAVDWSP---DG--QRVASGGKDKCLRIW 191
[Close]
BLAST of notchless-1 vs. Ensembl Human
Match: POC1B (POC1 centriolar protein B [Source:HGNC Symbol;Acc:HGNC:30836])

HSP 1 Score: 103.99 bits (258), Expect = 6.883e-23
Identity = 92/368 (25.00%), Postives = 137/368 (37.23%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            HK+ +  L  SPNGK LA++S       W      W+ K  A   +    K +V S+ + P        LLA+A  D+T+ +W             H   VR + +      + +  ED  I+VW         S+  H+HW+           R   F+P                                   +V CS+D T+ +W  + +NK      +      N V F+P G  IASA  D++VK+WD    K L   Q H   VN IS+      L++ S D ++K+  + + R                               L+  L GH   VF + +S  G + ASGG D  V LW
Sbjct:   17 HKAAITSLDLSPNGKQLATAS-------WDTFLMLWNFKPHARAYRYVGHKDVVTSVQFSP-----HGNLLASASRDRTVRLWIPDKRGKFSEFKAHTAPVRSVDFSADGQFLATASEDKSIKVWSMYRQRFLYSLYRHTHWV-----------RCAKFSP-------------------------------DGRLIVSCSEDKTIKIW--DTTNKQCVNNFSDSVGFANFVDFNPSGTCIASAGSDQTVKVWDVRVNKLLQHYQVHSGGVNCISFHPSGNYLITASSDGTLKILDLLEGR-------------------------------LIYTLQGHTGPVFTVSFSKGGELFASGGADTQVLLW 297

HSP 2 Score: 90.1225 bits (222), Expect = 2.191e-18
Identity = 105/408 (25.74%), Postives = 162/408 (39.71%), Query Frame = 3 
Query:  471 GHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPW-SLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARIS---HLLEALP----GHADSVFALDWSPDGMVV 1667
            GHK A+     SP+G+ LAT S D  +  W+       A     HK  V  + +SP+G +LAS+S    +  W     GK+S+      K +++  + V   A      DG  + LA A +DK+I +W     +   SL  H   VRC K+     LI S  ED  I++W   +             +N+ S +  +A  +  FNP                                   +     D T+ +W     NK +      H   VN + F P G ++ +AS D ++KI D + G+ + +LQGH   V  +S+S    L  S   D+ + LW            D+L       K +T  N KR       HLL+  P     H + V  ++ +P   V+
Sbjct:   16 GHKAAITSLDLSPNGKQLATASWDTFLMLWNFKPHA-RAYRYVGHKDVVTSVQFSPHGNLLASASRDRTVRLWIPDKRGKFSE-----FKAHTAPVRSVDFSA------DG--QFLATASEDKSIKVWSMYRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTN----------KQCVNNFSDSVGFANFVD-FNP-------------------------------SGTCIASAGSDQTVKVWD-VRVNK-LLQHYQVHSGGVNCISFHPSGNYLITASSDGTLKILDLLEGRLIYTLQGHTGPVFTVSFSKGGELFASGGADTQVLLWRTN--------FDELH-----CKGLTKRNLKRLHFDSPPHLLDIYPRTPHPHEEKVETVEINPKLEVI 352

HSP 3 Score: 58.151 bits (139), Expect = 3.179e-8
Identity = 48/190 (25.26%), Postives = 84/190 (44.21%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVL--LLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAE-DGVLCRSM 1007
            R   S+  H   V  A+FSPDGR + + S D T++ WD    T + C+ N   S      + ++P+G  +AS+     +  W +       + + L++        V  +++ P     +   L  A  D T+ I D +  +   +L GH   V  + +  G  L  SGG D ++ +WR   D + C+ +
Sbjct:  135 RFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWD---TTNKQCVNNFSDSVGFANFVDFNPSGTCIASAGSDQTVKVWDV-------RVNKLLQHYQVHSGGVNCISFHP-----SGNYLITASSDGTLKILDLLEGRLIYTLQGHTGPVFTVSFSKGGELFASGGADTQVLLWRTNFDELHCKGL 309

HSP 4 Score: 56.9954 bits (136), Expect = 7.662e-8
Identity = 53/232 (22.84%), Postives = 98/232 (42.24%), Query Frame = 3 
Query:  459 SSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRAL 1148
            S    H   V    FS DG++LAT S D +++ W +  Q     +   H   V    +SP+G+++ S S    I  W   N +        +   S +      + + P    GTC  +A+A  D+T+ +WD  + +       H   V C+ +  S N + +   D  +++    +G L  +++ H+  + ++S +    +   G  +      +TN + L  + L KR L
Sbjct:   96 SEFKAHTAPVRSVDFSADGQFLATASEDKSIKVWSMYRQRFLYSLYR-HTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTNKQ-------CVNNFSDSVGFANFVDFNP---SGTC--IASAGSDQTVKVWDVRVNKLLQHYQVHSGGVNCISFHPSGNYLITASSDGTLKILDLLEGRLIYTLQGHTGPVFTVSFSKGGELFASGGADTQVLLWRTNFDELHCKGLTKRNL 314

HSP 5 Score: 54.299 bits (129), Expect = 5.378e-7
Identity = 36/156 (23.08%), Postives = 68/156 (43.59%), Query Frame = 3 
Query: 1269 GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT------MAQIRGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            GH+  +  +  SP+G  +A+AS+D  + +W+           GH   V  + +S    LL S S+D +++LW        ++ +    P+  +      +   TA   K  ++        L +L  H   V    +SPDG ++ S  +D  +++W
Sbjct:   16 GHKAAITSLDLSPNGKQLATASWDTFLMLWNFKPHARAYRYVGHKDVVTSVQFSPHGNLLASASRDRTVRLWIPDKRGKFSEFKAHTAPVRSVDFSADGQFLATASEDKSIKVWSMYRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIW 171
[Close]
BLAST of notchless-1 vs. Ensembl Celegans
Match: W07E6.2 (pep chromosome:WBcel235:II:480722:484386:-1 gene:WBGene00021074.1 transcript:W07E6.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:W07E6.2)

HSP 1 Score: 395.971 bits (1016), Expect = 3.031e-132
Identity = 208/453 (45.92%), Postives = 282/453 (62.25%), Query Frame = 3 
Query:  351 VNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTP-EACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARR-NGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V+  +++ E    ET L++VY PQ+VFRVRP+TRC++S+PGH + V+ AQFSPDGR LA+GS D T+R WD+  + P   C   SHKS VL ++WSP+   +AS+   GEI  W   N K  ++    +K++   K+ + SLAW+P+H D TCRLLA+   D  I IWDT+       LSGH  SV C++WGG  LIYSG +D  +++WRA+DGV+CR+M  H+HWIN+++LNTDYA+R   F P    +K  I     EE QK A   Y  A    G ER+V  SDD TL +W P+E+ ++I  RMTGH  +VN V FSPD  +IASASFD+SVK+W G TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K++ +                               +   L   LPGH D VF +DWSP+G  V SGGKD +++LW
Sbjct:   63 VDSIRKSLEEIDFETTLKLVYQPQAVFRVRPVTRCSASIPGHGEPVISAQFSPDGRGLASGSGDQTMRIWDIELELPLHTC--KSHKSWVLCIAWSPDATKIASACKNGEICIW---NAKTGEQIGKTLKRH---KQWITSLAWQPMHKDPTCRLLASCGKDGNIFIWDTVQGTVVRCLSGHTASVTCLRWGGEGLIYSGSQDRTVKMWRADDGVMCRNMTGHAHWINTLALNTDYALRTSCFEP----SKRCIKPDTVEECQKVAQTRYEAALEIAGGERLVSGSDDFTLFMWNPKETKQSI-NRMTGHMQLVNQVVFSPDTRYIASASFDKSVKLWCGRTGKYLASLRGHVGPVYQVAWSADSRLLVSGSADSTLKVFEL-------------------------------KTKSLYYDLPGHGDEVFTVDWSPEGTKVVSGGKDKVLKLW 471
[Close]
BLAST of notchless-1 vs. Ensembl Celegans
Match: W07E6.2 (pep chromosome:WBcel235:II:480722:483104:-1 gene:WBGene00021074.1 transcript:W07E6.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:W07E6.2)

HSP 1 Score: 136.346 bits (342), Expect = 1.003e-37
Identity = 71/161 (44.10%), Postives = 96/161 (59.63%), Query Frame = 3 
Query: 1221 LWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +W P+E+ ++I  RMTGH  +VN V FSPD  +IASASFD+SVK+W G TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K++ +                               +   L   LPGH D VF +DWSP+G  V SGGKD +++LW
Sbjct:    1 MWNPKETKQSI-NRMTGHMQLVNQVVFSPDTRYIASASFDKSVKLWCGRTGKYLASLRGHVGPVYQVAWSADSRLLVSGSADSTLKVFEL-------------------------------KTKSLYYDLPGHGDEVFTVDWSPEGTKVVSGGKDKVLKLW 129
[Close]
BLAST of notchless-1 vs. Ensembl Celegans
Match: wdr-5.1 (WD repeat-containing protein wdr-5.1 [Source:UniProtKB/Swiss-Prot;Acc:Q17963])

HSP 1 Score: 83.5741 bits (205), Expect = 5.031e-17
Identity = 54/167 (32.34%), Postives = 85/167 (50.90%), Query Frame = 3 
Query: 1245 KAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            K +C  + GH   ++  KFSP G ++ ++S D++VKIW+        +L GH   VN I+WS DSR +VS S D ++K++ +   R                  P   L    SF + +   + K       ++ LP H+D V A+ ++ DG ++ASG  DGLVR+W
Sbjct:   78 KLMC-TLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGM---CIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIW 240

HSP 2 Score: 72.7886 bits (177), Expect = 1.884e-13
Identity = 73/285 (25.61%), Postives = 133/285 (46.67%), Query Frame = 3 
Query:  588 CMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKL-VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYA-IRIGAFNPMYE----STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDV--KFS-PDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEV 1412
            C    H  ++    +SP GK L +SS    +  W++ +         + ++  +  KL V  +AW         R + +A DDKT+ I++ +  +   +L GH   V C  +   S+L+ SG  D  +R+W  + G+  +++  HS  ++++S N D + I  G+++ +      +    I  L  +E    A + ++    NG + ++  + D+TL LW  + S      + TGH+N    +   FS   G +I S S D  + IW+  T + +  L+GH Q V
Sbjct:   81 CTLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDH--------MICERTLTGHKLGVNDIAWS-----SDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVKFSP---NG-KYILASNLDSTLKLW--DFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQPV 346

HSP 3 Score: 70.8626 bits (172), Expect = 6.998e-13
Identity = 61/222 (27.48%), Postives = 101/222 (45.50%), Query Frame = 3 
Query: 1104 NINNLDAEE-LQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMT----GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            N++++  E  L    L + + A  + +  +V  SDD TL +++       +  RMT    GH N V    F+P    + S SFD SV+IWD  TG  + +L  H   V+ +S++ D  L+ S S D  +++W  A  + +   +D     ++F K   +PN K    S+L              L+   GH +S   +FA      G  + SG +D  + +W
Sbjct:  115 NMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFE------IVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVK--FSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIW 328

HSP 4 Score: 65.855 bits (159), Expect = 2.557e-11
Identity = 50/198 (25.25%), Postives = 86/198 (43.43%), Query Frame = 3 
Query:  447 TRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCM----KWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSH 1022
            +R T ++ GH   V    F+P    + +GS D +VR WD+ T     C++   +H   V  +S++ +G ++AS S  G +  W   NG   Q    L+   +     VK             + + A+  D T+ +WD    +     +GH  S  C+       G   I SG EDC+I +W  +   + + ++ H+ 
Sbjct:  161 SRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGM---CIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANG---QCIKTLVDDENPPVAFVK--------FSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQ 344

HSP 5 Score: 64.3142 bits (155), Expect = 8.447e-11
Identity = 62/294 (21.09%), Postives = 128/294 (43.54%), Query Frame = 3 
Query:  879 SLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSL-QGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--LVPIDQLKEDISFKKKIT--------APNCK----RARISHLLEALPGHADSVFALDWSPDGMVVASGG--KDGLVRLW 1703
            +L GH KS+   K+      + +   D  +++W  +  +  R++  H   +N I+ ++D    + A +         + +   + L+     ++       +  +V  S D ++ +W   +    +C + +  H + V+ V F+ DG  IAS S+D  V+IWD   G+ + +L       V  + +S + + +++ + DS++KLW  ++ + L      +  K  I     +T        + +CK      +   +++ L GH   V A D  P   ++ASG    D  + +W
Sbjct:   82 TLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIW---DVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVKFSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQPVLASDCHPVQNIIASGALEPDNKIHIW 372
[Close]
BLAST of notchless-1 vs. Ensembl Celegans
Match: wdr-5.1 (WD repeat-containing protein wdr-5.1 [Source:UniProtKB/Swiss-Prot;Acc:Q17963])

HSP 1 Score: 83.5741 bits (205), Expect = 5.031e-17
Identity = 54/167 (32.34%), Postives = 85/167 (50.90%), Query Frame = 3 
Query: 1245 KAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            K +C  + GH   ++  KFSP G ++ ++S D++VKIW+        +L GH   VN I+WS DSR +VS S D ++K++ +   R                  P   L    SF + +   + K       ++ LP H+D V A+ ++ DG ++ASG  DGLVR+W
Sbjct:   78 KLMC-TLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGM---CIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIW 240

HSP 2 Score: 72.7886 bits (177), Expect = 1.884e-13
Identity = 73/285 (25.61%), Postives = 133/285 (46.67%), Query Frame = 3 
Query:  588 CMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKL-VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYA-IRIGAFNPMYE----STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDV--KFS-PDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEV 1412
            C    H  ++    +SP GK L +SS    +  W++ +         + ++  +  KL V  +AW         R + +A DDKT+ I++ +  +   +L GH   V C  +   S+L+ SG  D  +R+W  + G+  +++  HS  ++++S N D + I  G+++ +      +    I  L  +E    A + ++    NG + ++  + D+TL LW  + S      + TGH+N    +   FS   G +I S S D  + IW+  T + +  L+GH Q V
Sbjct:   81 CTLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDH--------MICERTLTGHKLGVNDIAWS-----SDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVKFSP---NG-KYILASNLDSTLKLW--DFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQPV 346

HSP 3 Score: 70.8626 bits (172), Expect = 6.998e-13
Identity = 61/222 (27.48%), Postives = 101/222 (45.50%), Query Frame = 3 
Query: 1104 NINNLDAEE-LQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMT----GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            N++++  E  L    L + + A  + +  +V  SDD TL +++       +  RMT    GH N V    F+P    + S SFD SV+IWD  TG  + +L  H   V+ +S++ D  L+ S S D  +++W  A  + +   +D     ++F K   +PN K    S+L              L+   GH +S   +FA      G  + SG +D  + +W
Sbjct:  115 NMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFE------IVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVK--FSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIW 328

HSP 4 Score: 65.855 bits (159), Expect = 2.557e-11
Identity = 50/198 (25.25%), Postives = 86/198 (43.43%), Query Frame = 3 
Query:  447 TRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCM----KWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSH 1022
            +R T ++ GH   V    F+P    + +GS D +VR WD+ T     C++   +H   V  +S++ +G ++AS S  G +  W   NG   Q    L+   +     VK             + + A+  D T+ +WD    +     +GH  S  C+       G   I SG EDC+I +W  +   + + ++ H+ 
Sbjct:  161 SRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIWDVKTGM---CIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANG---QCIKTLVDDENPPVAFVK--------FSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQ 344

HSP 5 Score: 64.3142 bits (155), Expect = 8.447e-11
Identity = 62/294 (21.09%), Postives = 128/294 (43.54%), Query Frame = 3 
Query:  879 SLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSL-QGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--LVPIDQLKEDISFKKKIT--------APNCK----RARISHLLEALPGHADSVFALDWSPDGMVVASGG--KDGLVRLW 1703
            +L GH KS+   K+      + +   D  +++W  +  +  R++  H   +N I+ ++D    + A +         + +   + L+     ++       +  +V  S D ++ +W   +    +C + +  H + V+ V F+ DG  IAS S+D  V+IWD   G+ + +L       V  + +S + + +++ + DS++KLW  ++ + L      +  K  I     +T        + +CK      +   +++ L GH   V A D  P   ++ASG    D  + +W
Sbjct:   82 TLEGHTKSISSAKFSPCGKYLGTSSADKTVKIWNMDHMICERTLTGHKLGVNDIAWSSDSRCVVSASDDKTLKIFEIVTSRMTKTLKGHNNYVFCCNFNPQSSLVVSGSFDESVRIW---DVKTGMCIKTLPAHSDPVSAVSFNRDGSLIASGSYDGLVRIWDTANGQCIKTLVDDENPPVAFVKFSPNGKYILASNLDSTLKLWDFSKGKTLKQYTGHENSKYCIFANFSVTGGKWIISGSEDCKIYIWNLQTREIVQCLEGHTQPVLASDCHPVQNIIASGALEPDNKIHIW 372
[Close]
BLAST of notchless-1 vs. Ensembl Celegans
Match: rack-1 (Guanine nucleotide-binding protein subunit beta-2-like 1 [Source:UniProtKB/Swiss-Prot;Acc:Q21215])

HSP 1 Score: 81.2629 bits (199), Expect = 1.633e-16
Identity = 66/210 (31.43%), Postives = 99/210 (47.14%), Query Frame = 3 
Query: 1161 TARRNGAERMVVCSDDNTLSLWKPEES---NKAICGR----MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW-TMAQIRGLLVPIDQLKED----ISFKKKITAP-----------------NCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            T  RN    ++  S D T+ +W  +     ++   GR    +TGH + V+DV  S DG F  S S+D+++++WD   G        H ++V  +++S D+R +VS S+D SIKLW T+AQ +  +   D    D    + F      P                 NC R + +H+     GH   V  +  SPDG + ASGGKDG   LW
Sbjct:   24 TYTRNDKTTVLSSSRDKTILVWDVDSVAPVDEGPIGRPVRSLTGHNHFVSDVVISSDGQFALSGSWDKTLRLWDLNQGVSTRQFISHTKDVLSVAFSADNRQIVSGSRDKSIKLWNTLAQCKYTIT--DDCHTDWVSTVRFSPSNRDPVIVSAGWDKVVKVWNLGNC-RLKTNHI-----GHTGYVNTVTVSPDGSLCASGGKDGQAMLW 225

HSP 2 Score: 57.7658 bits (138), Expect = 8.615e-9
Identity = 75/314 (23.89%), Postives = 127/314 (40.45%), Query Frame = 3 
Query:  831 DKTITIWDT---------ILCQPKISLSGHIKSVR-CMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD--------YAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAER---MVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDIS--FKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            DKTI +WD           + +P  SL+GH   V   +         SG  D  +R+W    GV  R    H+  + S++ + D            I  +N + +   T  ++   + +        +T R + + R   +V    D  + +W     N  +     GH   VN V  SPDG   AS   D    +WD   GK L +L G+   +N +S+S + R  +  +  SSIK+W +   +     I++LK +I+     + ++P C                    +L WS DG  + +G  D ++R++
Sbjct:   39 DKTILVWDVDSVAPVDEGPIGRPVRSLTGHNHFVSDVVISSDGQFALSGSWDKTLRLWDLNQGVSTRQFISHTKDVLSVAFSADNRQIVSGSRDKSIKLWNTLAQCKYTITDDCHTDWV--------STVRFSPSNRDPVIVSAGWDKVVKVW--NLGNCRLKTNHIGHTGYVNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLPGN-DVINAMSFSPN-RYWLCAAVGSSIKIWDLEDKK----EIEELKPEIASSGSSRGSSPQC-------------------ISLAWSQDGQTLFAGYTDNIIRVY 317

HSP 3 Score: 55.8398 bits (133), Expect = 3.878e-8
Identity = 52/203 (25.62%), Postives = 91/203 (44.83%), Query Frame = 3 
Query:  441 PITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLN 1046
            PI R   S+ GH   V     S DG++  +GS D T+R WDLN Q        SH  +VL +++S + + + S S    I  W+ +              +      V ++ + P + D    ++ +A  DK + +W+   C+ K +  GH   V  +      +L  SGG+D +  +W   +G    ++ P +  IN++S +
Sbjct:   57 PIGRPVRSLTGHNHFVSDVVISSDGQFALSGSWDKTLRLWDLN-QGVSTRQFISHTKDVLSVAFSADNRQIVSGSRDKSIKLWNTL------AQCKYTITDDCHTDWVSTVRFSPSNRD---PVIVSAGWDKVVKVWNLGNCRLKTNHIGHTGYVNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTL-PGNDVINAMSFS 248
[Close]
BLAST of notchless-1 vs. Ensembl Fly
Match: Nle (gene:FBgn0021874 transcript:FBtr0078075)

HSP 1 Score: 408.683 bits (1049), Expect = 7.776e-137
Identity = 222/542 (40.96%), Postives = 316/542 (58.30%), Query Frame = 3 
Query:   81 TVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPE-ACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            T   I+ + V   G   G    +P+    + L    N +L+ E  E + Y FF+            G + I  ++E+++                 +  +   E V+ IVY PQ+VF+VRP+TRCTSSMPGH +AV+   FSPDG +LA+GS D TVR WDLNT+TP   C    HK  VL +SW+P+GK LAS    G II W    G+  QK   L    S  KK +  LAW+P H D  CR LA+A  D    IWD  L Q  ++++GH  +V  ++WGG+ LIY+  +D  +++WRA DG+LCR+   H+HW+N+I+L+TDY +R G F+P+ + +K+++ +L  EELQ+ AL  Y     +  E +V CSDDNTL LW+  + NK +  RMTGHQNVVNDVK+SPD   IASASFD+SV++W    G+++ + +GH+Q V  ++WS DSRL+VS S+DS++K+W++                               +   L + LPGHAD VF +DW+PDG  VASGGKD +++LW
Sbjct:   18 TPHTIQARLVSDTGEEAGPPIDLPAGITTQQLGLICNALLKNE--EATPYLFFV------------GEDEIKKSLEDTL-----------------DLASVDTENVIDIVYQPQAVFKVRPVTRCTSSMPGHAEAVVSLNFSPDGAHLASGSGDTTVRLWDLNTETPHFTC--TGHKQWVLCVSWAPDGKRLASGCKAGSIIIWDPETGQ--QKGRPL----SGHKKHINCLAWEPYHRDPECRKLASASGDGDCRIWDVKLGQCLMNIAGHTNAVTAVRWGGAGLIYTSSKDRTVKMWRAADGILCRTFSGHAHWVNNIALSTDYVLRTGPFHPVKDRSKSHL-SLSTEELQESALKRYQAVCPDEVESLVSCSDDNTLYLWRNNQ-NKCV-ERMTGHQNVVNDVKYSPDVKLIASASFDKSVRLWRASDGQYMATFRGHVQAVYTVAWSADSRLIVSGSKDSTLKVWSV-------------------------------QTKKLAQELPGHADEVFGVDWAPDGSRVASGGKDKVIKLW 486
[Close]
BLAST of notchless-1 vs. Ensembl Fly
Match: wds (gene:FBgn0040066 transcript:FBtr0308566)

HSP 1 Score: 96.2857 bits (238), Expect = 2.935e-21
Identity = 55/161 (34.16%), Postives = 85/161 (52.80%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + GH   V+ VKFSP+G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DSRLLVS S D ++K+W ++  + L                P   L    SF + +        R    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   68 LAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESV---RIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 225

HSP 2 Score: 83.9593 bits (206), Expect = 3.985e-17
Identity = 75/251 (29.88%), Postives = 109/251 (43.43%), Query Frame = 3 
Query: 1005 MKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
              P+  W+ S S   D  I+I GA++  +E T           +    L I + A  + +  +V  SDD TL +W  E S       + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   80 FSPNGEWLASSS--ADKLIKIWGAYDGKFEKT-----------ISGHKLGISDVAWSSDSRLLVSGSDDKTLKVW--ELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 313

HSP 3 Score: 68.5514 bits (166), Expect = 5.047e-12
Identity = 79/325 (24.31%), Postives = 137/325 (42.15%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            V+P      ++ GH KAV   +FSP+G +LA+ SAD  ++ W       E  + + HK  +  ++WS + ++L S S    +  W L  GK S KT   +K +S+    V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +  +K +  ++ GH + V      P    IASA+   D+++K+W
Sbjct:   58 VKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGK-SLKT---LKGHSN---YVFCCNFNP-----QSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--SKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLW 357
[Close]
BLAST of notchless-1 vs. Ensembl Fly
Match: wds (gene:FBgn0040066 transcript:FBtr0070438)

HSP 1 Score: 96.2857 bits (238), Expect = 2.935e-21
Identity = 55/161 (34.16%), Postives = 85/161 (52.80%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + GH   V+ VKFSP+G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DSRLLVS S D ++K+W ++  + L                P   L    SF + +        R    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   68 LAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESV---RIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 225

HSP 2 Score: 83.9593 bits (206), Expect = 3.985e-17
Identity = 75/251 (29.88%), Postives = 109/251 (43.43%), Query Frame = 3 
Query: 1005 MKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
              P+  W+ S S   D  I+I GA++  +E T           +    L I + A  + +  +V  SDD TL +W  E S       + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   80 FSPNGEWLASSS--ADKLIKIWGAYDGKFEKT-----------ISGHKLGISDVAWSSDSRLLVSGSDDKTLKVW--ELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 313

HSP 3 Score: 68.5514 bits (166), Expect = 5.047e-12
Identity = 79/325 (24.31%), Postives = 137/325 (42.15%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            V+P      ++ GH KAV   +FSP+G +LA+ SAD  ++ W       E  + + HK  +  ++WS + ++L S S    +  W L  GK S KT   +K +S+    V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +  +K +  ++ GH + V      P    IASA+   D+++K+W
Sbjct:   58 VKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGK-SLKT---LKGHSN---YVFCCNFNP-----QSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--SKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLW 357
[Close]
BLAST of notchless-1 vs. Ensembl Fly
Match: Poc1 (gene:FBgn0036354 transcript:FBtr0075863)

HSP 1 Score: 92.0485 bits (227), Expect = 1.047e-19
Identity = 87/356 (24.44%), Postives = 140/356 (39.33%), Query Frame = 3 
Query:  420 QSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            Q +FR   + R      GH   +   +F PDG  +AT S D+TV  W+LN Q        SH + V  ++WSP G ++AS+     +  W                +  S + +  S A + +  D T  L+  A DDK+  IW     Q   S +     VR  K+     L+ +  +D  +R++  + G   R+                 A R  A++P          N+ A  L    + I++ +     +  VV                         H   VNDV F P G F+ S S DR+++I D + G+ + +L GH   VN +++S D     +   D  + +W
Sbjct:    2 QGLFRDPALER---HFTGHSGGITQLRFGPDGAQIATSSTDSTVILWNLN-QAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWE------------PKLRGVSGEFVAHSKAVRSVDFDSTGHLMLTASDDKSAKIWRVARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIYDVDSGECVRTFT-----------EERAAPRQLAWHPW--------GNMLAVALGCNRIKIFDVSGSQLLQLYVV-------------------------HSAPVNDVAFHPSGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVW 297

HSP 2 Score: 69.3218 bits (168), Expect = 3.016e-12
Identity = 68/309 (22.01%), Postives = 122/309 (39.48%), Query Frame = 3 
Query:  813 LAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMA--QIRGLLVPIDQLKEDISFKKK----ITAPNCKRARISHLLEALP-----GHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +A +  D T+ +W+       I  + H   V  + W    NL+ S G D  +++W  +   +      HS  + S+                ++ST                              M+  SDD +  +W+   + +          N V   KFSP+G  +A+AS D+SV+I+D  +G+ + +          ++W     +L      + IK++ ++  Q+  L V       D++F       ++  + +  RI  LLE  P     GH D+V A+ +S DG   A+GG D  + +W
Sbjct:   33 IATSSTDSTVILWNLNQAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFVAHSKAVRSVD---------------FDST---------------------------GHLMLTASDDKSAKIWR--VARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIYDVDSGECVRTFTEERAAPRQLAWHPWGNMLAVALGCNRIKIFDVSGSQLLQLYVVHSAPVNDVAFHPSGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVW 297

HSP 3 Score: 63.5438 bits (153), Expect = 1.957e-10
Identity = 38/112 (33.93%), Postives = 62/112 (55.36%), Query Frame = 3 
Query: 1173 NGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD----GMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQ 1496
            +GA+ +   S D+T+ LW   ++  A C R   H   VN V +SP G  +ASA  DR+VKIW+    G++G+F+     H + V  + +     L+++ S D S K+W +A+
Sbjct:   29 DGAQ-IATSSTDSTVILWNLNQA--ARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFVA----HSKAVRSVDFDSTGHLMLTASDDKSAKIWRVAR 133

HSP 4 Score: 62.7734 bits (151), Expect = 4.346e-10
Identity = 41/165 (24.85%), Postives = 75/165 (45.45%), Query Frame = 3 
Query: 1242 NKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT--MAQIRGLLVPIDQLKEDISFKKK----ITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + A+    TGH   +  ++F PDG  IA++S D +V +W+       +    H   VN ++WS    L+ S   D ++K+W   +  + G  V   +    + F       +TA + K A+I        + +     + V +  +SP+G +VA+   D  VR++
Sbjct:    7 DPALERHFTGHSGGITQLRFGPDGAQIATSSTDSTVILWNLNQAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFVAHSKAVRSVDFDSTGHLMLTASDDKSAKIWRVARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIY 171

HSP 5 Score: 54.299 bits (129), Expect = 2.144e-7
Identity = 43/168 (25.60%), Postives = 80/168 (47.62%), Query Frame = 3 
Query:  486 VLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRA 980
            V  A+FSP+G+ +AT S D +VR +D+++     C+      ++    L+W P G +LA +     I  + +         S L++        V  +A+ P     +   L +  DD+TI I D +  +P  +L+GH  +V  + +    +   +GG D ++ VW++
Sbjct:  147 VRSAKFSPNGKLVATASDDKSVRIYDVDSGE---CVRTFTEERAAPRQLAWHPWGNMLAVALGCNRIKIFDV-------SGSQLLQLYVVHSAPVNDVAFHP-----SGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVWQS 299
[Close]
BLAST of notchless-1 vs. Ensembl Fly
Match: Poc1 (gene:FBgn0036354 transcript:FBtr0321318)

HSP 1 Score: 92.0485 bits (227), Expect = 1.299e-19
Identity = 87/356 (24.44%), Postives = 140/356 (39.33%), Query Frame = 3 
Query:  420 QSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            Q +FR   + R      GH   +   +F PDG  +AT S D+TV  W+LN Q        SH + V  ++WSP G ++AS+     +  W                +  S + +  S A + +  D T  L+  A DDK+  IW     Q   S +     VR  K+     L+ +  +D  +R++  + G   R+                 A R  A++P          N+ A  L    + I++ +     +  VV                         H   VNDV F P G F+ S S DR+++I D + G+ + +L GH   VN +++S D     +   D  + +W
Sbjct:    2 QGLFRDPALER---HFTGHSGGITQLRFGPDGAQIATSSTDSTVILWNLN-QAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWE------------PKLRGVSGEFVAHSKAVRSVDFDSTGHLMLTASDDKSAKIWRVARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIYDVDSGECVRTFTEER-----------AAPRQLAWHPW--------GNMLAVALGCNRIKIFDVSGSQLLQLYVV-------------------------HSAPVNDVAFHPSGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVW 297

HSP 2 Score: 68.9366 bits (167), Expect = 4.779e-12
Identity = 68/309 (22.01%), Postives = 122/309 (39.48%), Query Frame = 3 
Query:  813 LAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMA--QIRGLLVPIDQLKEDISFKKK----ITAPNCKRARISHLLEALP-----GHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +A +  D T+ +W+       I  + H   V  + W    NL+ S G D  +++W  +   +      HS  + S+                ++ST                              M+  SDD +  +W+   + +          N V   KFSP+G  +A+AS D+SV+I+D  +G+ + +          ++W     +L      + IK++ ++  Q+  L V       D++F       ++  + +  RI  LLE  P     GH D+V A+ +S DG   A+GG D  + +W
Sbjct:   33 IATSSTDSTVILWNLNQAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFVAHSKAVRSVD---------------FDST---------------------------GHLMLTASDDKSAKIWRV--ARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIYDVDSGECVRTFTEERAAPRQLAWHPWGNMLAVALGCNRIKIFDVSGSQLLQLYVVHSAPVNDVAFHPSGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVW 297

HSP 3 Score: 63.1586 bits (152), Expect = 2.952e-10
Identity = 38/112 (33.93%), Postives = 62/112 (55.36%), Query Frame = 3 
Query: 1173 NGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD----GMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQ 1496
            +GA+ +   S D+T+ LW   ++  A C R   H   VN V +SP G  +ASA  DR+VKIW+    G++G+F+     H + V  + +     L+++ S D S K+W +A+
Sbjct:   29 DGAQ-IATSSTDSTVILWNLNQA--ARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFV----AHSKAVRSVDFDSTGHLMLTASDDKSAKIWRVAR 133

HSP 4 Score: 62.3882 bits (150), Expect = 5.290e-10
Identity = 41/165 (24.85%), Postives = 75/165 (45.45%), Query Frame = 3 
Query: 1242 NKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT--MAQIRGLLVPIDQLKEDISFKKK----ITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + A+    TGH   +  ++F PDG  IA++S D +V +W+       +    H   VN ++WS    L+ S   D ++K+W   +  + G  V   +    + F       +TA + K A+I        + +     + V +  +SP+G +VA+   D  VR++
Sbjct:    7 DPALERHFTGHSGGITQLRFGPDGAQIATSSTDSTVILWNLNQAARCIRFASHSAPVNGVAWSPKGNLVASAGHDRTVKIWEPKLRGVSGEFVAHSKAVRSVDFDSTGHLMLTASDDKSAKIWRVARRQFVSSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIY 171

HSP 5 Score: 53.9138 bits (128), Expect = 2.294e-7
Identity = 45/177 (25.42%), Postives = 82/177 (46.33%), Query Frame = 3 
Query:  459 SSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRA 980
            SS       V  A+FSP+G+ +AT S D +VR +D+++     C+      ++    L+W P G +LA +     I  + +         S L++        V  +A+ P     +   L +  DD+TI I D +  +P  +L+GH  +V  + +    +   +GG D ++ VW++
Sbjct:  138 SSFAQQNNWVRSAKFSPNGKLVATASDDKSVRIYDVDSGE---CVRTFTEERAAPRQLAWHPWGNMLAVALGCNRIKIFDV-------SGSQLLQLYVVHSAPVNDVAFHP-----SGHFLLSGSDDRTIRILDLLEGRPIYTLTGHTDAVNAVAFSRDGDKFATGGSDRQLLVWQS 299
[Close]
BLAST of notchless-1 vs. Ensembl Zebrafish
Match: nle1 (notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-050522-321])

HSP 1 Score: 406.371 bits (1043), Expect = 5.760e-136
Identity = 225/542 (41.51%), Postives = 305/542 (56.27%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V  + +Q  D  G   G  F +P   +   L    N +L+KE P   +   F   + E           +V+++ + I                   +  G+ET  VL +VY PQ+VFRVR + RCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W  + GK   KT       +   K +  L W+PLHL+  CR LA+   D TI IWDT+L      L+GH  SV C+KWGG  L+Y+  +D  I+VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P   +          EE++++AL  YN+ R  G ER+V  SDD T+ LW P E  K +  R+TGHQ +VN+V FSPD   IASASFD+S+KIWDG TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K+W +                               +   L   LPGHAD VFA+DWSPDG  VASGGKD  +R+W
Sbjct:    5 VERVLIQLQDEAGEVLGSPFDVPLDISPDKLQLVCNALLQKEEP---VPFLFFVKDAE-----------LVSSLGSCI-------------------QTLGLETEQVLPVVYQPQAVFRVRAVARCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHH-TSRGHTHWVLSIAWSPDGKKLASGCKNSQIFLWDPVTGKQIGKTL------TGHTKWITWLCWEPLHLNPECRYLASTSKDCTIRIWDTVLGHYDKILTGHTHSVTCVKWGGDGLLYTSSQDRTIKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPANATINPQDLTGSLEEIKEKALKRYNSVRGEGHERLVSGSDDFTMFLWNPAEDKKPV-ARLTGHQALVNEVLFSPDTRLIASASFDKSIKIWDGKTGKYLNSLRGHVGPVYQVAWSADSRLLVSGSSDSTLKVWDI-------------------------------KTGKLNADLPGHADEVFAVDWSPDGQRVASGGKDKCLRIW 474
[Close]
BLAST of notchless-1 vs. Ensembl Zebrafish
Match: nle1 (notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-050522-321])

HSP 1 Score: 406.371 bits (1043), Expect = 5.760e-136
Identity = 225/542 (41.51%), Postives = 305/542 (56.27%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V  + +Q  D  G   G  F +P   +   L    N +L+KE P   +   F   + E           +V+++ + I                   +  G+ET  VL +VY PQ+VFRVR + RCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W  + GK   KT       +   K +  L W+PLHL+  CR LA+   D TI IWDT+L      L+GH  SV C+KWGG  L+Y+  +D  I+VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P   +          EE++++AL  YN+ R  G ER+V  SDD T+ LW P E  K +  R+TGHQ +VN+V FSPD   IASASFD+S+KIWDG TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K+W +                               +   L   LPGHAD VFA+DWSPDG  VASGGKD  +R+W
Sbjct:    5 VERVLIQLQDEAGEVLGSPFDVPLDISPDKLQLVCNALLQKEEP---VPFLFFVKDAE-----------LVSSLGSCI-------------------QTLGLETEQVLPVVYQPQAVFRVRAVARCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHH-TSRGHTHWVLSIAWSPDGKKLASGCKNSQIFLWDPVTGKQIGKTL------TGHTKWITWLCWEPLHLNPECRYLASTSKDCTIRIWDTVLGHYDKILTGHTHSVTCVKWGGDGLLYTSSQDRTIKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPANATINPQDLTGSLEEIKEKALKRYNSVRGEGHERLVSGSDDFTMFLWNPAEDKKPV-ARLTGHQALVNEVLFSPDTRLIASASFDKSIKIWDGKTGKYLNSLRGHVGPVYQVAWSADSRLLVSGSSDSTLKVWDI-------------------------------KTGKLNADLPGHADEVFAVDWSPDGQRVASGGKDKCLRIW 474
[Close]
BLAST of notchless-1 vs. Ensembl Zebrafish
Match: nle1 (notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:ZDB-GENE-050522-321])

HSP 1 Score: 406.371 bits (1043), Expect = 5.760e-136
Identity = 225/542 (41.51%), Postives = 305/542 (56.27%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V  + +Q  D  G   G  F +P   +   L    N +L+KE P   +   F   + E           +V+++ + I                   +  G+ET  VL +VY PQ+VFRVR + RCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W  + GK   KT       +   K +  L W+PLHL+  CR LA+   D TI IWDT+L      L+GH  SV C+KWGG  L+Y+  +D  I+VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P   +          EE++++AL  YN+ R  G ER+V  SDD T+ LW P E  K +  R+TGHQ +VN+V FSPD   IASASFD+S+KIWDG TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K+W +                               +   L   LPGHAD VFA+DWSPDG  VASGGKD  +R+W
Sbjct:    5 VERVLIQLQDEAGEVLGSPFDVPLDISPDKLQLVCNALLQKEEP---VPFLFFVKDAE-----------LVSSLGSCI-------------------QTLGLETEQVLPVVYQPQAVFRVRAVARCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHH-TSRGHTHWVLSIAWSPDGKKLASGCKNSQIFLWDPVTGKQIGKTL------TGHTKWITWLCWEPLHLNPECRYLASTSKDCTIRIWDTVLGHYDKILTGHTHSVTCVKWGGDGLLYTSSQDRTIKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPANATINPQDLTGSLEEIKEKALKRYNSVRGEGHERLVSGSDDFTMFLWNPAEDKKPV-ARLTGHQALVNEVLFSPDTRLIASASFDKSIKIWDGKTGKYLNSLRGHVGPVYQVAWSADSRLLVSGSSDSTLKVWDI-------------------------------KTGKLNADLPGHADEVFAVDWSPDGQRVASGGKDKCLRIW 474
[Close]
BLAST of notchless-1 vs. Ensembl Zebrafish
Match: wdr5 (WD repeat domain 5 [Source:ZFIN;Acc:ZDB-GENE-040426-2082])

HSP 1 Score: 96.6709 bits (239), Expect = 2.175e-21
Identity = 56/172 (32.56%), Postives = 89/172 (51.74%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N  +   + GH   V+ VKFSP G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DS LLVS S D ++K+W ++  + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   30 PVKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 198

HSP 2 Score: 85.1149 bits (209), Expect = 1.524e-17
Identity = 80/295 (27.12%), Postives = 123/295 (41.69%), Query Frame = 3 
Query:  873 KISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            K +L+GH K+V  +K+  S   + S   D  I++W A DG   +++  H   I+ ++ ++D                   +NL                       +V  SDD TL +W  + S+      + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   38 KFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSD-------------------SNL-----------------------LVSASDDKTLKIW--DVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 286

HSP 3 Score: 69.3218 bits (168), Expect = 3.091e-12
Identity = 76/325 (23.38%), Postives = 131/325 (40.31%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            V+P      ++ GH KAV   +FSP G +LA+ SAD  ++ W       E  + + HK  +  ++WS +  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +   K I  ++ GH +VV      P    IASA+   D+++K+W
Sbjct:   31 VKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--TKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW 330
[Close]
BLAST of notchless-1 vs. Ensembl Zebrafish
Match: daw1 (dynein assembly factor with WDR repeat domains 1 [Source:NCBI gene;Acc:556760])

HSP 1 Score: 97.8265 bits (242), Expect = 2.733e-21
Identity = 101/411 (24.57%), Postives = 178/411 (43.31%), Query Frame = 3 
Query:  561 DLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG--SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLD----------AEELQKRA-------LLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT------MAQIRGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            DL   TPE  +E       L+L       ++ SS V    +    +  K SQK         + +  +  L     +  G+C  +  +YD +T  IWDT   +   +L GH   V  + +     + + +G  D   ++W AE G    + + H+  I  +           AFNP  +ST     ++D           EE+   A        L +NT      +R+V  S D+T  LW      K     ++GH+  ++ V+F+ D   IA+AS D+S K+WD   G+ L +L GH  EV  + ++   +L+ + S D + ++++      + Q+ G    I ++  +    + +TA   K +R+        L+ L GH+D +F+  ++ +G  + +G KD   R+W
Sbjct:   31 DLLNLTPETDVEE------LVLEIRAAEPLITSSCVEQVKLLICKLQEKVSQKDERKFYLFRALQAHILPLTNVAFNKSGSC-FITGSYD-RTCKIWDTASGEELHTLEGHRNVVYAIAFNNPYGDKVATGSFDKTCKLWSAETGKCFYTFRGHTAEIVCL-----------AFNP--QSTLVATGSMDTTAKLWDVESGEEVSTLAGHFAEIISLCFNTT----GDRLVTGSFDHTAILWDVPSGRKV--HVLSGHRGEISCVQFNWDCSLIATASLDKSCKVWDAEGGQCLATLLGHNDEVLDVCFNYTGQLIATASADGTSRVFSTDTFQCLCQLEGHKGEISKVCFNAQGSRVLTASVDKTSRVWCVKTGACLQVLEGHSDEIFSCAFNYEGDTIITGSKDNTCRIW 414

HSP 2 Score: 69.3218 bits (168), Expect = 4.492e-12
Identity = 76/350 (21.71%), Postives = 147/350 (42.00%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSW-SPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSV--RCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALL------IYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            ++  H   +    F+  G    TGS D T + WD  +      +E  H++ V  +++ +P G  +A+ S       WS   GK          +  +A+  +  LA+ P        L+A    D T  +WD    +   +L+GH   +   C    G  L+ +G  D    +W    G     +  H   I+ +  N D ++   A      S   +    DAE  Q  A L      + +       + +   S D T  ++   ++ + +C ++ GH+  ++ V F+  G  + +AS D++ ++W   TG  L  L+GH  E+   +++ +   +++ S+D++ ++W
Sbjct:   87 ALQAHILPLTNVAFNKSGSCFITGSYDRTCKIWDTASGEELHTLEG-HRNVVYAIAFNNPYGDKVATGSFDKTCKLWSAETGK-----CFYTFRGHTAE--IVCLAFNPQS-----TLVATGSMDTTAKLWDVESGEEVSTLAGHFAEIISLCFNTTGDRLV-TGSFDHTAILWDVPSGRKVHVLSGHRGEISCVQFNWDCSLIATA------SLDKSCKVWDAEGGQCLATLLGHNDEVLDVCFNYTGQLIATASADGTSRVFST-DTFQCLC-QLEGHKGEISKVCFNAQGSRVLTASVDKTSRVWCVKTGACLQVLEGHSDEIFSCAFNYEGDTIITGSKDNTCRIW 414

HSP 3 Score: 56.9954 bits (136), Expect = 3.358e-8
Identity = 57/261 (21.84%), Postives = 100/261 (38.31%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVR--CMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLW 1226
            +C  +  GH   ++   F+P    +ATGS D T + WD+ +   E      H + ++ L ++  G  L + S     I W + +G+     S    + S  +       W        C L+A A  DK+  +WD    Q   +L GH   V   C  + G  LI +   D   RV+  +       ++ H   I+ +  N   +  + A                 + L+  +  I++ A     + ++  S DNT  +W
Sbjct:  168 KCFYTFRGHTAEIVCLAFNPQSTLVATGSMDTTAKLWDVESG-EEVSTLAGHFAEIISLCFNTTGDRLVTGSFDHTAILWDVPSGRKVHVLSGHRGEISCVQ-----FNWD-------CSLIATASLDKSCKVWDAEGGQCLATLLGHNDEVLDVCFNYTG-QLIATASADGTSRVFSTDTFQCLCQLEGHKGEISKVCFNAQGSRVLTASVDKTSRVWCVKTGACLQVLEGHSDEIFSCAFNYEGDTIITGSKDNTCRIW 414
[Close]
BLAST of notchless-1 vs. Ensembl Xenopus
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:779995])

HSP 1 Score: 417.542 bits (1072), Expect = 2.950e-140
Identity = 226/544 (41.54%), Postives = 314/544 (57.72%), Query Frame = 3 
Query:   72 INDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +N+ V  + +QF D +G   G  F +P       L    N +L++E P   +   F  +++E           IVT+++ ++                   ++   E V+ I+Y PQ+VF+VR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDLNT+TP       H   +L ++WSP+G+ LAS     +I  W    GK       + K  +   K +  L W+PLHL+  CR LA+A  D TI IWDT++ Q +  L+ H +SV  +KWGG  L+YS  +D  I+ WRA+DGVLCR+++ H+HW+N+++L+TDY +R GAFNP   S      +   E+L+++AL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N+V FSPD   IASASFD+SVK+WDG TGKFL SL+GH+  V  I+WS DSRLLVS S DS++K+W                                ++   LL  LPGHAD V+++DWSPDG  VASGGKD  +R+W
Sbjct:    1 MNEDVGRLLIQFKDENGEVLGSPFDVPVDITSDKLQLVCNALLQEEEP---VPLAFFVHDLE-----------IVTSLDKTL-----------------EKQSVETEKVIDIIYQPQAVFKVRAVTRCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLNTETPH-FTSKGHTHWILSIAWSPDGRKLASGCKNSQIFIWDPNTGK------QIGKPLTGHSKWITWLCWEPLHLNPECRYLASASKDCTIRIWDTVMGQCQKILTSHTQSVTAVKWGGDGLLYSSSQDRTIKAWRAQDGVLCRTLQGHAHWVNTMALSTDYVLRTGAFNPADASVNPQDMSGSLEDLKEKALKRYNEVRGQGPERLVSGSDDFTLFLWAPAEEKKPL-QRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFLASLRGHVSAVYQIAWSADSRLLVSGSSDSTLKVWD-------------------------------SKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRIW 474

HSP 2 Score: 50.8322 bits (120), Expect = 4.611e-6
Identity = 40/153 (26.14%), Postives = 70/153 (45.75%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W    +         H++ +  + +SP+ +++AS+S    +  W    GK+     A ++ + SA   V  +AW         RLL +   D T+ +WD+   +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:  335 LVSGSDDFTLFLWAPAEEKKPLQRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFL----ASLRGHVSA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDSKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRIWR 475
[Close]
BLAST of notchless-1 vs. Ensembl Xenopus
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:779995])

HSP 1 Score: 414.846 bits (1065), Expect = 4.043e-139
Identity = 225/543 (41.44%), Postives = 313/543 (57.64%), Query Frame = 3 
Query:   72 INDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            +N+ V  + +QF D +G   G  F +P       L    N +L++E P   +   F  +++E           IVT+++ ++                   ++   E V+ I+Y PQ+VF+VR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDLNT+TP       H   +L ++WSP+G+ LAS     +I  W    GK       + K  +   K +  L W+PLHL+  CR LA+A  D TI IWDT++ Q +  L+ H +SV  +KWGG  L+YS  +D  I+ WRA+DGVLCR+++ H+HW+N+++L+TDY +R GAFNP   S      +   E+L+++AL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N+V FSPD   IASASFD+SVK+WDG TGKFL SL+GH+  V  I+WS DSRLLVS S DS++K+W                                ++   LL  LPGHAD V+++DWSPDG  VASGGKD  +R+
Sbjct:    1 MNEDVGRLLIQFKDENGEVLGSPFDVPVDITSDKLQLVCNALLQEEEP---VPLAFFVHDLE-----------IVTSLDKTL-----------------EKQSVETEKVIDIIYQPQAVFKVRAVTRCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLNTETPH-FTSKGHTHWILSIAWSPDGRKLASGCKNSQIFIWDPNTGK------QIGKPLTGHSKWITWLCWEPLHLNPECRYLASASKDCTIRIWDTVMGQCQKILTSHTQSVTAVKWGGDGLLYSSSQDRTIKAWRAQDGVLCRTLQGHAHWVNTMALSTDYVLRTGAFNPADASVNPQDMSGSLEDLKEKALKRYNEVRGQGPERLVSGSDDFTLFLWAPAEEKKPL-QRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFLASLRGHVSAVYQIAWSADSRLLVSGSSDSTLKVWD-------------------------------SKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRM 473
[Close]
BLAST of notchless-1 vs. Ensembl Xenopus
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:779995])

HSP 1 Score: 414.846 bits (1065), Expect = 4.518e-139
Identity = 225/544 (41.36%), Postives = 313/544 (57.54%), Query Frame = 3 
Query:   72 INDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +N+ V  + +QF D +G   G  F +P       L    N +L+    E+ +   F  +++E           IVT+++ ++                   ++   E V+ I+Y PQ+VF+VR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDLNT+TP       H   +L ++WSP+G+ LAS     +I  W    GK       + K  +   K +  L W+PLHL+  CR LA+A  D TI IWDT++ Q +  L+ H +SV  +KWGG  L+YS  +D  I+ WRA+DGVLCR+++ H+HW+N+++L+TDY +R GAFNP   S      +   E+L+++AL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N+V FSPD   IASASFD+SVK+WDG TGKFL SL+GH+  V  I+WS DSRLLVS S DS++K+W                                ++   LL  LPGHAD V+++DWSPDG  VASGGKD  +R+W
Sbjct:   12 MNEDVGRLLIQFKDENGEVLGSPFDVPVDITSDKLQLVCNALLQ----EEPVPLAFFVHDLE-----------IVTSLDKTL-----------------EKQSVETEKVIDIIYQPQAVFKVRAVTRCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLNTETPH-FTSKGHTHWILSIAWSPDGRKLASGCKNSQIFIWDPNTGK------QIGKPLTGHSKWITWLCWEPLHLNPECRYLASASKDCTIRIWDTVMGQCQKILTSHTQSVTAVKWGGDGLLYSSSQDRTIKAWRAQDGVLCRTLQGHAHWVNTMALSTDYVLRTGAFNPADASVNPQDMSGSLEDLKEKALKRYNEVRGQGPERLVSGSDDFTLFLWAPAEEKKPL-QRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFLASLRGHVSAVYQIAWSADSRLLVSGSSDSTLKVWD-------------------------------SKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRIW 484

HSP 2 Score: 50.8322 bits (120), Expect = 5.707e-6
Identity = 40/153 (26.14%), Postives = 70/153 (45.75%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W    +         H++ +  + +SP+ +++AS+S    +  W    GK+     A ++ + SA   V  +AW         RLL +   D T+ +WD+   +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:  345 LVSGSDDFTLFLWAPAEEKKPLQRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFL----ASLRGHVSA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDSKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRIWR 485
[Close]
BLAST of notchless-1 vs. Ensembl Xenopus
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:779995])

HSP 1 Score: 414.461 bits (1064), Expect = 6.107e-139
Identity = 225/543 (41.44%), Postives = 313/543 (57.64%), Query Frame = 3 
Query:   72 INDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            +N+ V  + +QF D +G   G  F +P       L    N +L++E P   +   F  +++E           IVT+++ ++                   ++   E V+ I+Y PQ+VF+VR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDLNT+TP       H   +L ++WSP+G+ LAS     +I  W    GK       + K  +   K +  L W+PLHL+  CR LA+A  D TI IWDT++ Q +  L+ H +SV  +KWGG  L+YS  +D  I+ WRA+DGVLCR+++ H+HW+N+++L+TDY +R GAFNP   S      +   E+L+++AL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N+V FSPD   IASASFD+SVK+WDG TGKFL SL+GH+  V  I+WS DSRLLVS S DS++K+W                                ++   LL  LPGHAD V+++DWSPDG  VASGGKD  +R+
Sbjct:    5 MNEDVGRLLIQFKDENGEVLGSPFDVPVDITSDKLQLVCNALLQEEEP---VPLAFFVHDLE-----------IVTSLDKTL-----------------EKQSVETEKVIDIIYQPQAVFKVRAVTRCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLNTETPH-FTSKGHTHWILSIAWSPDGRKLASGCKNSQIFIWDPNTGK------QIGKPLTGHSKWITWLCWEPLHLNPECRYLASASKDCTIRIWDTVMGQCQKILTSHTQSVTAVKWGGDGLLYSSSQDRTIKAWRAQDGVLCRTLQGHAHWVNTMALSTDYVLRTGAFNPADASVNPQDMSGSLEDLKEKALKRYNEVRGQGPERLVSGSDDFTLFLWAPAEEKKPL-QRMTGHQALINEVLFSPDTRIIASASFDKSVKLWDGKTGKFLASLRGHVSAVYQIAWSADSRLLVSGSSDSTLKVWD-------------------------------SKTKKLLVDLPGHADEVYSVDWSPDGQRVASGGKDKCLRM 477
[Close]
BLAST of notchless-1 vs. Ensembl Xenopus
Match: daw1 (dynein assembly factor with WDR repeat domains 1 [Source:NCBI gene;Acc:394975])

HSP 1 Score: 102.834 bits (255), Expect = 9.296e-23
Identity = 73/305 (23.93%), Postives = 147/305 (48.20%), Query Frame = 3 
Query:  831 DKTITIWDTILCQPKISLSGHIKSVRCMKWGG--SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYA-IRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT------MAQIRGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            D+T  +WDT   +   +L GH   V  +++     + I +G  D   ++W AE G    + + H+  I  ++ N     I  G+ +   +      +  +A  L   A  I + +     +R++  S D+T+S+W+     +     + GH+  ++  +F+ D   IA+AS D+S K+WD + GK + +L GH  EV  +++    +L+ + S D + ++++      +A++ G    I ++  +    + +TA + K +R+        L+ L GH D +F+  ++ +G  + +G KD   R+W
Sbjct:  135 DRTCKVWDTASGEELHTLEGHRNVVYAIQFNNPYGDKIATGSFDKTCKLWSAETGKCYHTFRGHTAEIVCLAFNPQSTLIATGSMDTTAKLWDIQ-SGEEALTLSGHAAEIISLSFNTTGDRLITGSFDHTVSVWEIPSGRRN--HTLIGHRGEISSAQFNWDCSLIATASMDKSCKLWDSLNGKCVATLTGHEDEVLDVTFDSTGQLVATASADGTARVYSASSRKCLAKLEGHEGEISKICFNAQGNRILTASSDKTSRLWNPHTGECLQVLKGHTDEIFSCAFNYEGNTIITGSKDNTCRIW 436

HSP 2 Score: 70.8626 bits (172), Expect = 1.957e-12
Identity = 69/345 (20.00%), Postives = 149/345 (43.19%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFS-PDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            ++ GH+  V   QF+ P G  +ATGS D T + W   T     C      H + ++ L+++P   ++A+ S+      W + +G+      AL     +A+  + SL++         RL+  ++ D T+++W+    +   +L GH   +   ++    +LI +   D   ++W + +G    ++  H   +                +  ++ST                  +  TA  +G  R+   S    L+             ++ GH+  ++ + F+  G  I +AS D++ ++W+  TG+ L  L+GH  E+   +++ +   +++ S+D++ ++W
Sbjct:  151 TLEGHRNVVYAIQFNNPYGDKIATGSFDKTCKLWSAETG---KCYHTFRGHTAEIVCLAFNPQSTLIATGSMDTTAKLWDIQSGE-----EALTLSGHAAE--IISLSFNTTGD----RLITGSF-DHTVSVWEIPSGRRNHTLIGHRGEISSAQFNWDCSLIATASMDKSCKLWDSLNGKCVATLTGHEDEV---------------LDVTFDSTGQ----------------LVATASADGTARVYSASSRKCLA-------------KLEGHEGEISKICFNAQGNRILTASSDKTSRLWNPHTGECLQVLKGHTDEIFSCAFNYEGNTIITGSKDNTCRIW 436

HSP 3 Score: 68.9366 bits (167), Expect = 1.013e-11
Identity = 65/305 (21.31%), Postives = 116/305 (38.03%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIW 1358
            +C  +  GH   ++   F+P    +ATGS D T + WD+ +   EA   + H + ++ LS++  G  L + S    +  W + +G+ +        + SSA+       W        C L+A A  DK+  +WD++  +   +L+GH   V  + +  +  L+ +   D   RV+ A        ++ H   I+ I  N                                              R++  S D T  LW P       C + + GH + +    F+ +G  I + S D + +IW
Sbjct:  190 KCYHTFRGHTAEIVCLAFNPQSTLIATGSMDTTAKLWDIQSG-EEALTLSGHAAEIISLSFNTTGDRLITGSFDHTVSVWEIPSGRRNHTLIGHRGEISSAQ-----FNWD-------CSLIATASMDKSCKLWDSLNGKCVATLTGHEDEVLDVTFDSTGQLVATASADGTARVYSASSRKCLAKLEGHEGEISKICFNAQ------------------------------------------GNRILTASSDKTSRLWNPHTGE---CLQVLKGHTDEIFSCAFNYEGNTIITGSKDNTCRIW 436
[Close]
BLAST of notchless-1 vs. Ensembl Mouse
Match: Nle1 (notchless homolog 1 [Source:MGI Symbol;Acc:MGI:2429770])

HSP 1 Score: 409.838 bits (1052), Expect = 3.320e-137
Identity = 220/540 (40.74%), Postives = 310/540 (57.41%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V+ + VQF D  G   G  F +P       L    N +L +E P   +   F  ++ E           IV+++  ++                  S++   E ++ I+Y PQ+VFRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+GK LAS    G+++ W    G    +T       +   K +  L+W+PLH++  CR +A++  D ++ +WDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   +          +EL++RA   YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:   14 VQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEEP---LPLAFYVHDAE-----------IVSSLGKTL-----------------ESQSVETEKIVDIIYQPQAVFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKG-HRHWVLSISWSPDGKKLASGCKNGQVLLWDPSTGLQVGRTL------TGHSKWITGLSWEPLHMNPECRYVASSSKDGSVRVWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEATVNAQDLQGSLKELKERASSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLA-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 483

HSP 2 Score: 51.2174 bits (121), Expect = 3.299e-6
Identity = 40/153 (26.14%), Postives = 67/153 (43.79%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W              H++ +  + +SP+ +++AS+S    I  W    GK+     A ++ + +A   V  +AW         RLL +   D T+ +WD    +    L GH   V  + W      + SGG+D  +R+WR
Sbjct:  344 LVSGSDDFTLFLWSPAEDKKPLARMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYL----ASLRGHVAA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDVKAQKLATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 484
[Close]
BLAST of notchless-1 vs. Ensembl Mouse
Match: Poc1b (POC1 centriolar protein B [Source:MGI Symbol;Acc:MGI:1918511])

HSP 1 Score: 100.523 bits (249), Expect = 1.348e-22
Identity = 92/368 (25.00%), Postives = 138/368 (37.50%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            HK+ +    +SPN K +A++S       W      WS K  A   +    K +V SL + P        LLA+A  D+T+ +W             H   VR + +     L+ +  ED  I+VW         S+  H+HW+           R   F+P                                   +V CS+D T+ +W  + +NK      +      N V F+P+G  IASA  D +VKIWD    K L   Q H   VN +S+      LV+ S D ++K+  + + R                               L+  L GH   VF + +S DG ++ SGG D  V +W
Sbjct:   17 HKAAITSADFSPNCKQIATAS-------WDTFLMLWSLKPHARAYRYVGHKDVVTSLQFSP-----QGNLLASASRDRTVRLWVLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWV-----------RCAKFSP-------------------------------DGRLIVSCSEDKTIKIW--DTTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDIRMNKLLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGR-------------------------------LIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 2 Score: 90.8929 bits (224), Expect = 2.231e-19
Identity = 88/342 (25.73%), Postives = 137/342 (40.06%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            S  GHK A+  A FSP+ + +AT S D  +  W L      A     HK  V  L +SP G +LAS+S    +  W             L +K  S++    +   + +      +LL  A +DK+I +W     +   SL  H   VRC K+     LI S  ED  I++W   +             +N+ S +  +A  +  FNP                              NG   +     D+ + +W    +   +      H   VN + F P G  + +AS D +VK+ D + G+ + +LQGH   V  +S+S D  LL S   D+ + +W
Sbjct:   13 SFKGHKAAITSADFSPNCKQIATASWDTFLMLWSLKPHA-RAYRYVGHKDVVTSLQFSPQGNLLASASRDRTVRLW------------VLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTN----------KQCVNNFSDSVGFANFVD-FNP------------------------------NGTC-IASAGSDHAVKIWDIRMNK--LLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 3 Score: 59.6918 bits (143), Expect = 4.118e-9
Identity = 51/188 (27.13%), Postives = 84/188 (44.68%), Query Frame = 3 
Query:  423 SVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVL--LLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            S+FR R +     S+  H   V  A+FSPDGR + + S D T++ WD    T + C+ N   S      + ++PNG  +AS+     +  W +       + + L++        V  L++ PL        L  A  D T+ + D I  +   +L GH   V  + +     L+ SGG D ++ +WR
Sbjct:  130 SMFRQRFLY----SLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWD---TTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDI-------RMNKLLQHYQVHSCGVNCLSFHPL-----GNSLVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIWR 298
[Close]
BLAST of notchless-1 vs. Ensembl Mouse
Match: Poc1b (POC1 centriolar protein B [Source:MGI Symbol;Acc:MGI:1918511])

HSP 1 Score: 102.064 bits (253), Expect = 1.503e-22
Identity = 92/368 (25.00%), Postives = 138/368 (37.50%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            HK+ +    +SPN K +A++S       W      WS K  A   +    K +V SL + P        LLA+A  D+T+ +W             H   VR + +     L+ +  ED  I+VW         S+  H+HW+           R   F+P                                   +V CS+D T+ +W  + +NK      +      N V F+P+G  IASA  D +VKIWD    K L   Q H   VN +S+      LV+ S D ++K+  + + R                               L+  L GH   VF + +S DG ++ SGG D  V +W
Sbjct:   17 HKAAITSADFSPNCKQIATAS-------WDTFLMLWSLKPHARAYRYVGHKDVVTSLQFSP-----QGNLLASASRDRTVRLWVLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWV-----------RCAKFSP-------------------------------DGRLIVSCSEDKTIKIW--DTTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDIRMNKLLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGR-------------------------------LIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 2 Score: 91.6633 bits (226), Expect = 3.657e-19
Identity = 88/342 (25.73%), Postives = 137/342 (40.06%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            S  GHK A+  A FSP+ + +AT S D  +  W L      A     HK  V  L +SP G +LAS+S    +  W             L +K  S++    +   + +      +LL  A +DK+I +W     +   SL  H   VRC K+     LI S  ED  I++W   +             +N+ S +  +A  +  FNP                              NG   +     D+ + +W    +   +      H   VN + F P G  + +AS D +VK+ D + G+ + +LQGH   V  +S+S D  LL S   D+ + +W
Sbjct:   13 SFKGHKAAITSADFSPNCKQIATASWDTFLMLWSLKPHA-RAYRYVGHKDVVTSLQFSPQGNLLASASRDRTVRLW------------VLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTN----------KQCVNNFSDSVGFANFVD-FNP------------------------------NGT-CIASAGSDHAVKIWDIRMNK--LLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 3 Score: 59.3066 bits (142), Expect = 7.468e-9
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 3 
Query:  423 SVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVL--LLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            S+FR R +     S+  H   V  A+FSPDGR + + S D T++ WD    T + C+ N   S      + ++PNG  +AS+     +  W +       + + L++        V  L++ PL   G    L  A  D T+ + D I  +   +L GH   V  + +     L+ SGG D ++ +WR
Sbjct:  130 SMFRQRFLY----SLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWD---TTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDI-------RMNKLLQHYQVHSCGVNCLSFHPL---GNS--LVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIWR 298
[Close]
BLAST of notchless-1 vs. Ensembl Mouse
Match: Poc1b (POC1 centriolar protein B [Source:MGI Symbol;Acc:MGI:1918511])

HSP 1 Score: 102.064 bits (253), Expect = 1.629e-22
Identity = 92/368 (25.00%), Postives = 138/368 (37.50%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            HK+ +    +SPN K +A++S       W      WS K  A   +    K +V SL + P        LLA+A  D+T+ +W             H   VR + +     L+ +  ED  I+VW         S+  H+HW+           R   F+P                                   +V CS+D T+ +W  + +NK      +      N V F+P+G  IASA  D +VKIWD    K L   Q H   VN +S+      LV+ S D ++K+  + + R                               L+  L GH   VF + +S DG ++ SGG D  V +W
Sbjct:   17 HKAAITSADFSPNCKQIATAS-------WDTFLMLWSLKPHARAYRYVGHKDVVTSLQFSP-----QGNLLASASRDRTVRLWVLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWV-----------RCAKFSP-------------------------------DGRLIVSCSEDKTIKIW--DTTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDIRMNKLLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGR-------------------------------LIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 2 Score: 91.6633 bits (226), Expect = 4.904e-19
Identity = 88/342 (25.73%), Postives = 137/342 (40.06%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            S  GHK A+  A FSP+ + +AT S D  +  W L      A     HK  V  L +SP G +LAS+S    +  W             L +K  S++    +   + +      +LL  A +DK+I +W     +   SL  H   VRC K+     LI S  ED  I++W   +             +N+ S +  +A  +  FNP                              NG   +     D+ + +W  +     +      H   VN + F P G  + +AS D +VK+ D + G+ + +LQGH   V  +S+S D  LL S   D+ + +W
Sbjct:   13 SFKGHKAAITSADFSPNCKQIATASWDTFLMLWSLKPHA-RAYRYVGHKDVVTSLQFSPQGNLLASASRDRTVRLW------------VLDRKGKSSEFKAHTAPVRSVDFSADGQLLVTASEDKSIKVWSMFRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTN----------KQCVNNFSDSVGFANFVD-FNP------------------------------NGT-CIASAGSDHAVKIW--DIRMNKLLQHYQVHSCGVNCLSFHPLGNSLVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIW 297

HSP 3 Score: 59.3066 bits (142), Expect = 9.583e-9
Identity = 52/188 (27.66%), Postives = 85/188 (45.21%), Query Frame = 3 
Query:  423 SVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVL--LLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            S+FR R +     S+  H   V  A+FSPDGR + + S D T++ WD    T + C+ N   S      + ++PNG  +AS+     +  W +       + + L++        V  L++ PL   G    L  A  D T+ + D I  +   +L GH   V  + +     L+ SGG D ++ +WR
Sbjct:  130 SMFRQRFLY----SLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWD---TTNKQCVNNFSDSVGFANFVDFNPNGTCIASAGSDHAVKIWDI-------RMNKLLQHYQVHSCGVNCLSFHPL---GNS--LVTASSDGTVKMLDLIEGRLIYTLQGHTGPVFTVSFSKDGELLTSGGADAQVLIWR 298
[Close]
BLAST of notchless-1 vs. Ensembl Mouse
Match: Wdr5 (WD repeat domain 5 [Source:MGI Symbol;Acc:MGI:2155884])

HSP 1 Score: 98.9821 bits (245), Expect = 3.787e-22
Identity = 57/172 (33.14%), Postives = 91/172 (52.91%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N A+   + GH   V+ VKFSP+G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DS LLVS S D ++K+W ++  + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   30 PVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 198

HSP 2 Score: 85.8853 bits (211), Expect = 9.273e-18
Identity = 75/251 (29.88%), Postives = 110/251 (43.82%), Query Frame = 3 
Query: 1005 MKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
              P+  W+ S S   D  I+I GA++  +E T           +    L I + A  + +  +V  SDD TL +W  + S+      + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D LV +W
Sbjct:   53 FSPNGEWLASSS--ADKLIKIWGAYDGKFEKT-----------ISGHKLGISDVAWSSDSNLLVSASDDKTLKIW--DVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIW 286

HSP 3 Score: 68.5514 bits (166), Expect = 4.795e-12
Identity = 76/325 (23.38%), Postives = 132/325 (40.62%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            V+P      ++ GH KAV   +FSP+G +LA+ SAD  ++ W       E  + + HK  +  ++WS +  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +   K I  ++ GH +VV      P    IASA+   D+++K+W
Sbjct:   31 VKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQ--TKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW 330
[Close]
BLAST of notchless-1 vs. UniProt/SwissProt
Match: sp|Q9NVX2|NLE1_HUMAN (Notchless protein homolog 1 OS=Homo sapiens OX=9606 GN=NLE1 PE=1 SV=4)

HSP 1 Score: 419.083 bits (1076), Expect = 6.111e-140
Identity = 227/546 (41.58%), Postives = 313/546 (57.33%), Query Frame = 3 
Query:   66 ESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            E++   V+ + VQF D  G   G  F +P       L    N +L +E P     +FF+ +              IV+++  ++                  S+    E VL I+Y PQ++FRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+G+ LAS    G+I+ W    GK   +T A         K +  L+W+PLH +  CR +A++  D ++ IWDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    8 EAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLP--LAFFVHDA------------EIVSSLGKTL-----------------ESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKG-HRHWVLSISWSPDGRKLASGCKNGQILLWDPSTGKQVGRTLA------GHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 483
[Close]
BLAST of notchless-1 vs. UniProt/SwissProt
Match: sp|Q58D20|NLE1_BOVIN (Notchless protein homolog 1 OS=Bos taurus OX=9913 GN=NLE1 PE=2 SV=3)

HSP 1 Score: 419.083 bits (1076), Expect = 6.314e-140
Identity = 228/540 (42.22%), Postives = 313/540 (57.96%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V+ + VQF D  G   G  F +P       L    N +L +E P   +   F  ++ E           IV+++  ++                  S+    E VL I+Y PQ++FRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+GK LAS    G+I+ W    GK  Q   AL   +    K + +L+W+PLH +  CR +A++  D ++ +WDT   + + +L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   IASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:   15 VQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEDP---LPLAFYVHDAE-----------IVSSLGRTL-----------------ESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCQG-HRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGK--QVGRALTGHS----KWITALSWEPLHANPECRYVASSSKDGSVRVWDTTAGRCERTLTGHAQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNAQDLRGSLQELKERALSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLA-RMTGHQALINQVVFSPDSRVIASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LSTDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 484

HSP 2 Score: 52.373 bits (124), Expect = 9.619e-6
Identity = 41/153 (26.80%), Postives = 67/153 (43.79%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W              H++ +  + +SP+ +V+AS+S    I  W    GK+     A ++ + +A   V  +AW         RLL +   D T+ +WD    +    L GH   V  + W      + SGG+D  +R+WR
Sbjct:  345 LVSGSDDFTLFLWSPAEDKKPLARMTGHQALINQVVFSPDSRVIASASFDKSIKLWDGRTGKYL----ASLRGHVAA---VYQIAWS-----ADSRLLVSGSSDSTLKVWDVKAQKLSTDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 485
[Close]
BLAST of notchless-1 vs. UniProt/SwissProt
Match: sp|Q5RFF8|NLE1_PONAB (Notchless protein homolog 1 OS=Pongo abelii OX=9601 GN=NLE1 PE=2 SV=3)

HSP 1 Score: 416.772 bits (1070), Expect = 5.677e-139
Identity = 226/540 (41.85%), Postives = 310/540 (57.41%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V+ + VQF D  G   G  F +P       L    N +L +E P     +FF+ +              I++++  ++                  S+    E VL I+Y PQ++FRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+GK LAS    G+I+ W    GK   +T A         K +  L+W+PLH +  CR +A++  D ++ IWDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   S          +EL++RAL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:   14 VQRLLVQFQDEGGQLLGSPFDVPVDITPDRLQLVCNALLAQEDPLP--LAFFVHDA------------EIISSLGKTL-----------------ESQAVETEKVLDIIYQPQAIFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKG-HRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGKQVGRTLA------GHSKWITGLSWEPLHANPECRYVASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLMRGQGPERLVSGSDDFTLFLWSPAEDKKPLT-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LAMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 483
[Close]
BLAST of notchless-1 vs. UniProt/SwissProt
Match: sp|Q8VEJ4|NLE1_MOUSE (Notchless protein homolog 1 OS=Mus musculus OX=10090 GN=Nle1 PE=1 SV=4)

HSP 1 Score: 409.838 bits (1052), Expect = 2.322e-136
Identity = 220/540 (40.74%), Postives = 310/540 (57.41%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V+ + VQF D  G   G  F +P       L    N +L +E P   +   F  ++ E           IV+++  ++                  S++   E ++ I+Y PQ+VFRVR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP    +  H+  VL +SWSP+GK LAS    G+++ W    G    +T       +   K +  L+W+PLH++  CR +A++  D ++ +WDT   + +  L+GH +SV C++WGG  L+YS  +D  I+VWRA DGVLCR+++ H HW+N+++L+TDYA+R GAF P   +          +EL++RA   YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N V FSPD   +ASASFD+S+K+WDG TGK+L SL+GH+  V  I+WS DSRLLVS S DS++K+W                 D+  +K              L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:   14 VQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEEP---LPLAFYVHDAE-----------IVSSLGKTL-----------------ESQSVETEKIVDIIYQPQAVFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKG-HRHWVLSISWSPDGKKLASGCKNGQVLLWDPSTGLQVGRTL------TGHSKWITGLSWEPLHMNPECRYVASSSKDGSVRVWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEATVNAQDLQGSLKELKERASSRYNLVRGQGPERLVSGSDDFTLFLWSPAEDKKPLA-RMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVW-----------------DVKAQK--------------LATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 483
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BLAST of notchless-1 vs. UniProt/SwissProt
Match: sp|Q7ZXK9|NLE1_XENLA (Notchless protein homolog 1 OS=Xenopus laevis OX=8355 GN=nle1 PE=2 SV=1)

HSP 1 Score: 408.297 bits (1048), Expect = 6.482e-136
Identity = 223/544 (40.99%), Postives = 310/544 (56.99%), Query Frame = 3 
Query:   72 INDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + + V  + +QF + +G   G  F +P       L    N +L++E P   +   F   ++E           IVT+++ ++                   ++   E V+ I+Y PQ+VF+VR +TRCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W    GK       + K  +   K +  L W+PLHL+   R LA+A  D TI IWDT++ Q +  L+ H +SV  +KWGG  L+YS  +D  I+ WRA+DGVLCR+++ H+HW+N+++L+TDY +R GAFNP   S      +   E L+++AL  YN  R  G ER+V  SDD TL LW P E  K +  RMTGHQ ++N+V FSPD   IASASFD+S+K+WDG TGKFL SL+GH+  V  I+WS DSRLLVS S DS++K+W                                ++   LL  LPGHAD V+++DWSPDG  VASGGKD  +R+W
Sbjct:    1 MKEDVGRLLIQFKNENGEGLGTPFDVPVDITPDKLQLVCNALLQEEDP---VPLAFFVQDLE-----------IVTSLDKTL-----------------EKQSVETEKVIDIIYQPQAVFKVRAVTRCTSSLEGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPH-FTSKGHTHWVLSIAWSPDGKKLASGCKNSQIFIWDPSTGK------QIGKPLTGHSKWITWLCWEPLHLNPESRYLASASKDCTIRIWDTVMGQCQKILTSHTQSVTAVKWGGDGLLYSSSQDRTIKAWRAQDGVLCRTLQGHAHWVNTMALSTDYVLRTGAFNPADASVNPQDMSGSLEVLKEKALKRYNEVRGQGPERLVSGSDDFTLFLWAPAEEKKPL-QRMTGHQALINEVLFSPDTRIIASASFDKSIKLWDGKTGKFLTSLRGHVSAVYQIAWSADSRLLVSGSSDSTLKVWD-------------------------------SKTKKLLIDLPGHADEVYSVDWSPDGQRVASGGKDKCLRIW 474
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BLAST of notchless-1 vs. TrEMBL
Match: I1ZIF1 (Notchless-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 1127.85 bits (2916), Expect = 0.000e+0
Identity = 544/546 (99.63%), Postives = 545/546 (99.82%), Query Frame = 3 
Query:   63 MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQS+YSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL
Sbjct:    1 MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSLYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENGHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 546

HSP 2 Score: 71.2478 bits (173), Expect = 4.219e-9
Identity = 34/36 (94.44%), Postives = 35/36 (97.22%), Query Frame = 2 
Query: 1691 RQIVEILVFEDFTVKYNYKIVLTFLTADCRLFFIYL 1798
            R +VEILVFEDFTVKYNYKIVLTFLTADCRLFFIYL
Sbjct:  545 RLLVEILVFEDFTVKYNYKIVLTFLTADCRLFFIYL 580
[Close]
BLAST of notchless-1 vs. TrEMBL
Match: A0A0R3T1V1 (WD_REPEATS_REGION domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS878 PE=4 SV=1)

HSP 1 Score: 492.656 bits (1267), Expect = 5.464e-165
Identity = 262/567 (46.21%), Postives = 354/567 (62.43%), Query Frame = 3 
Query:   87 RHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLIN-GKWS-QKTSALIKKN------SSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPM---------------YESTKT--NINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIR--GLLVPIDQLKEDISFKKKITAPNCKRA--RISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            R + +QFVD++G   G    +P SS+V  L++ +  + E +  E  +Y FFI            G   I+  + + I      +    V +T E+      E+V+++ Y  Q+VFRVRP+TRCTS++PGHK AVLVA FSPD R LATGS D++VRFWDLNT+ P+  + ++H S VL L+WSP+G  LAS    G I  W     G W     S LI+ +      +S  + ++SLAW+P HLD  CRLLAAAY D +I IWDT+  +P+  LSGH K V C++WGG++LIYS  +D  IRVWR+ DGVLCR +  H HW+N ++L+ DY +R G F+P                 E +KT  +   +  E ++ R   +Y   + +G+ERMV  SDDNTLSLW PE S   IC RMTGHQ VVNDVKFSPDG  IASASFD SVKIWDG+TG+FL ++ GH+Q+V L++WS DSRLLVS S+DS++KLWTMA++R  G    +   K     K+K+     K+   R  H+L  LPGHAD+V+ALDWSPDG  V SGGKD ++++
Sbjct:   24 RSVFIQFVDTNGEETGTPIFVPCSSSVSALTDILTTLRENDSNEPKVYEFFI------------GETQILDDLASGISQFTAAQKL--VQDTVES--GAITESVVKVTYQAQAVFRVRPVTRCTSTIPGHKGAVLVALFSPDARALATGSGDHSVRFWDLNTELPQNVVTSAHTSPVLCLAWSPDGLKLASGCQGGYIYLWKQDTVGNWILGNPSPLIQASLARTPANSKSRWIRSLAWRPFHLDRDCRLLAAAYQDTSIVIWDTVTGKPERILSGHEKPVTCLRWGGTDLIYSSSQDRTIRVWRSTDGVLCRRLDMHGHWVNCLALSVDYVLRTGPFDPADSVLVKPIIPATAQGEEKSKTLGSATFIKRELMRLRTKELYEKVKGSGSERMVAGSDDNTLSLWTPESSKNPICPRMTGHQGVVNDVKFSPDGRLIASASFDHSVKIWDGLTGQFLATMFGHVQDVYLVTWSSDSRLLVSGSKDSTLKLWTMAEVRRWGQEASVQMSKAK---KRKVDGGGLKQPHQRKRHILHDLPGHADAVYALDWSPDGQRVVSGGKDRVLKM 571
[Close]
BLAST of notchless-1 vs. TrEMBL
Match: A0A158QCT0 (WD_REPEATS_REGION domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS1907 PE=4 SV=1)

HSP 1 Score: 490.73 bits (1262), Expect = 2.410e-164
Identity = 257/559 (45.97%), Postives = 351/559 (62.79%), Query Frame = 3 
Query:   87 RHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSL-INGKWS--------QKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--------MYESTKTNINNLDA--EELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIR--GLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            R + +QF+D++G   G    +P SS+V  L++ +    E++  E  +Y FFI +      ++ + G +  T  +   ++ DK+++                E+V+++ Y  Q+VFRVRP+TRCTS++PGHK AVLV+ FSPD R LATGS D++VRFWDLNT+ P+  + N+H   VL L+WSP+G  LAS    G I  W     G W         Q + AL   NS ++  V+SLAW+P HLD  CR LAAAY + +I IWDT+  +P+  LSGH K V C++WGG++LIYS  +D  IRVWR+ DGVLCR +  H HWINS++L+ DY +R G F+P        +  ST       ++  E ++ RA  +Y+  + +G ER+V  SDDNTLSLW PE S   IC RMTGHQ VVNDVKFSPD   IASASFD SVKIWDG+TG+FL ++ GH+Q+V L++WS DSRLLVSCS+DS++KLWTMA++R  G    + + K                 R  HLL  LPGHAD+V+ALDWSPDG  V SGGKD ++++
Sbjct:   24 RSVFIQFIDTNGEETGTPVFVPCSSSVSALTDILTTFRERDPNEPEVYEFFIGDT--QILDDLAAGISQFTATQK--LDKDKIES------------GAITESVVKVTYQAQAVFRVRPVTRCTSTLPGHKGAVLVSLFSPDARALATGSGDHSVRFWDLNTELPQNVVTNAHAFPVLCLAWSPDGLKLASGCQGGYIYLWKQDTAGNWILENPSPLIQASLALTPANSKSR-WVRSLAWRPFHLDRDCRFLAAAYQETSIVIWDTVTGKPERLLSGHEKPVTCLRWGGTDLIYSSSQDRTIRVWRSTDGVLCRRLDMHGHWINSLALSVDYVLRTGPFDPADAVFVKPVIPSTAQGEEKSESSGELMRLRAKELYDKVKGSGPERLVAGSDDNTLSLWTPESSKNPICPRMTGHQGVVNDVKFSPDSRLIASASFDHSVKIWDGLTGQFLATMFGHVQDVYLVAWSSDSRLLVSCSKDSTLKLWTMAEVRRWGQEAAVKKSKAKKKKVDGDDDNKRPHQRKRHLLHDLPGHADAVYALDWSPDGQRVVSGGKDRVLKM 565
[Close]
BLAST of notchless-1 vs. TrEMBL
Match: A0A0X3PTC6 (Notchless protein homolog 1 OS=Schistocephalus solidus OX=70667 GN=NLE1 PE=4 SV=1)

HSP 1 Score: 490.73 bits (1262), Expect = 2.691e-164
Identity = 249/572 (43.53%), Postives = 349/572 (61.01%), Query Frame = 3 
Query:   66 ESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCG--IETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLI---NGKWS-QKTSALIKKN------SSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP-----MYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRA---------RISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +S++     + +QF D+DG   G    +P  ++V  L+  +  + + E     +Y FFI            G   ++  +  +I        F     T E ++     +E+V+++ Y  Q++FRVRP+TRCTS++PGHK AVLVA FSPD R LATGS D +VRFWDLNT+ P+A    +H S VL LSWSP+G  LAS    G I  W      NG W+    S LI+ +      +S  + ++SL WKP HLD  CR LAAAY + +I IWDT++ +P   LSGH K V C++WGG++LIYS  +D  IRVWR+ DGV+CR +  H HW+N ++L+ DY +R G F+P     +  +  T   +   +++Q RA  +Y   + +G ER+   SDDNTLSLW PE S K +C RMTGHQ VVNDVKFSPD   +ASASFD S+KIWDG TG+FL ++ GH+Q+V L++WS DSRLLVS S+DS++KLW+MA +R       +  ++ + +   TA   K++         R  H+L  LPGHAD+V+ALDWSPDG  V SGGKD ++++W
Sbjct:   22 KSVDAAEPSVFIQFADTDGEETGSPIYLPCKTDVSALTEILTSLRQSEPDTPEVYEFFI------------GETQVLDDLATAIAQ------FTASKNTTEGTDVVAEALESVVKVTYQAQALFRVRPVTRCTSTLPGHKGAVLVALFSPDARALATGSGDCSVRFWDLNTELPQAANTTAHGSPVLCLSWSPDGLRLASGCQGGYIYLWRQTGGPNGGWAIDPPSPLIQSSLARTPANSKSRWIRSLVWKPFHLDAECRYLAAAYQEASIIIWDTVMGKPHRILSGHEKPVTCLRWGGTDLIYSASQDRTIRVWRSTDGVICRRLDLHGHWVNCLALSVDYVLRTGPFDPADAVLVKTAPVTAEGDEKRKQMQLRAKELYEKVKGSGPERLAAGSDDNTLSLWSPETSKKTLCSRMTGHQGVVNDVKFSPDARLLASASFDHSIKIWDGHTGQFLATMFGHVQDVYLVAWSSDSRLLVSGSKDSTLKLWSMADVR-------RWNQEAAVQAVTTAKRAKKSNDGAKKVHQRRRHILHDLPGHADAVYALDWSPDGQRVVSGGKDRVLKIW 568
[Close]
BLAST of notchless-1 vs. TrEMBL
Match: A0A4E0RD44 (Notchless protein 1 OS=Fasciola hepatica OX=6192 GN=D915_003768 PE=4 SV=1)

HSP 1 Score: 490.73 bits (1262), Expect = 4.398e-164
Identity = 253/569 (44.46%), Postives = 353/569 (62.04%), Query Frame = 3 
Query:   81 TVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWS---LINGKWSQKTS------ALIKKNSSAK-KLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYE-----STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIR-----GLLVPIDQLKE-----DISFKKKITAPNCKRA---RISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +V  + VQ  DSDG   G    +P S N   L+  + +++EK+  + + Y F++            G   ++  +E +++         G +         G+E+V+++VY  Q+VFRVRP+TRCTS++PGHK AVLVAQFSPD R LATGS D TVRFWDL+T+ P     ++H + VL L+WSP+G  LAS    G +  W     + G WS  T        L+K  ++++ + ++SL W+P HLD  CR L  AY D ++ IWD+   QP ++L+GH K V C++WGG++L+YS  +D  IRVWR++DG+LCR++  H HW+N ++L+TDY +R G F+P        +     + +  EELQK+A  +Y   R  G+ER+V  SDDNTLSLW+PE   K +  RM GHQ VVNDVKFS D   IASASFD SVKIWDG TG+FL +L GH+QEV L++WS DSRLLVSCS+DS++KLW++ ++R        VP  + K+     D+   KK    N   A   R  HLL  LPGHAD+V+ALDWSPDG  VASGGKD ++++W
Sbjct:   29 SVNSVFVQLKDSDGNEAGSPVWLPLSINTTRLTALVKELVEKDEDDPARYDFYV------------GDIQLLDDLETALIQSRA----KGADPEVPQYGATGLESVVQVVYQEQAVFRVRPVTRCTSTLPGHKGAVLVAQFSPDARCLATGSGDQTVRFWDLSTELPATTGTDAHHAPVLCLAWSPDGLRLASGCQGGVVCLWEESDSVKGLWSMCTGKPLIRPTLVKSPTASRGRWIRSLVWRPFHLDPDCRKLVVAYQDTSMVIWDSWTGQPLLTLNGHEKPVVCVRWGGTDLLYSASQDRTIRVWRSDDGILCRTLSMHGHWVNCLALSTDYVLRTGPFDPAEAQLIKCAPPKKSDKMKREELQKQATELYGKIRGAGSERLVAGSDDNTLSLWQPELDKKPLAPRMVGHQGVVNDVKFSRDARMIASASFDHSVKIWDGHTGQFLATLFGHVQEVYLVAWSSDSRLLVSCSKDSTLKLWSLDEVRRWNQEAATVPKSERKQQLFAADVVGGKKAKGSNTNAAAHQRRRHLLHDLPGHADAVYALDWSPDGQRVASGGKDRVLKIW 581
[Close]
BLAST of notchless-1 vs. Ensembl Cavefish
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:103046643])

HSP 1 Score: 409.838 bits (1052), Expect = 1.883e-137
Identity = 226/544 (41.54%), Postives = 312/544 (57.35%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDA--EELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V  + +QF D  G   G  F +P       L    N +L+KE P   +   F   + E           +V+++  S+                   +  G+ET  VL +VY PQ+VFRVR + RCTSS+ GH +AV+   FSP G+YLA+GS D TVRFWDL T+TP       H   VL ++WSP+GK LAS     +I+ W  + G+       + K  +   K +  L W+PLHL+  CR LA++  D ++ IWDT+L +    L+GH +SV C+KWGG  L+Y+  +D  I+VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P   +   N+ +L    +E+++RAL  YN  R  G ER+V  SDD TL LW P E  K +  R+TGHQ +VN+V FSPD   +ASASFD+S+KIWDG TGK+L SL+GH+  V  ++WS DSRLLVS S DS++K+W         V   +L  D                       LPGHAD VFA+DWSPDG  VASGGKD  +R+W
Sbjct:    5 VERMLIQFQDEGGEALGSPFDVPLDITPDKLQLVCNALLQKEEP---VPLLFFVRDAE-----------VVSSLGASV-------------------QTLGLETEQVLPVVYQPQAVFRVRAVARCTSSLQGHTEAVISVAFSPTGKYLASGSGDTTVRFWDLTTETPHHTARG-HTHWVLSIAWSPDGKKLASGCKNSQILLWDPVTGQ------QIGKALTGHTKWITWLCWEPLHLNPECRYLASSSKDCSVRIWDTVLGRCDKILTGHTQSVTCVKWGGDGLLYTSSQDRTIKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPA--TATVNLQDLTGSLDEIKERALKRYNKVRGEGPERLVSGSDDFTLFLWNPAEEKKPV-ARLTGHQALVNEVLFSPDTRLLASASFDKSIKIWDGKTGKYLTSLRGHVAAVYQVAWSADSRLLVSGSSDSTLKVWD--------VKTGKLNVD-----------------------LPGHADEVFAVDWSPDGQRVASGGKDKCLRIW 474

HSP 2 Score: 52.7582 bits (125), Expect = 7.717e-7
Identity = 40/153 (26.14%), Postives = 65/153 (42.48%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W+   +         H++ V  + +SP+ ++LAS+S    I  W    GK+       +         V  +AW         RLL +   D T+ +WD    +  + L GH   V  + W      + SGG+D  +R+WR
Sbjct:  335 LVSGSDDFTLFLWNPAEEKKPVARLTGHQALVNEVLFSPDTRLLASASFDKSIKIWDGKTGKYLTSLRGHVAA-------VYQVAWS-----ADSRLLVSGSSDSTLKVWDVKTGKLNVDLPGHADEVFAVDWSPDGQRVASGGKDKCLRIWR 475
[Close]
BLAST of notchless-1 vs. Ensembl Cavefish
Match: ENSAMXT00000007963.2 (WD repeat-containing protein 5-like [Source:NCBI gene;Acc:103023146])

HSP 1 Score: 100.138 bits (248), Expect = 1.257e-22
Identity = 58/172 (33.72%), Postives = 89/172 (51.74%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N  +   + GH   ++ VKFSP+G ++AS+S D+ +KIW    GKF  SL GH   ++ ++WS DS LLVS S D ++K+W M   + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   27 PVKPNYTLKFTLAGHTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKFEKSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKC---LKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMW 195

HSP 2 Score: 89.3521 bits (220), Expect = 5.853e-19
Identity = 90/307 (29.32%), Postives = 145/307 (47.23%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKL-VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGA----FNPMYESTKTN-INNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRM-TGHQNVVNDV--KFS-PDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCS--QDSSIKLW 1484
            H   +  + +SPNG+ LASSS    I  W   +GK         +K+ S  KL +  +AW P        LL +A DDKT+ IWD    +   +L GH   V C  +   SNL+ SG  D  +R+W  + G   +++  HS  ++++  N D ++ + +       M++ST    +  L  ++    + + ++    NG + ++  + DNTL LW   + +K  C +  TGH+N    V   FS   G +I S S D  V IW+  T + +  LQGH   V   +      ++ S +   D +IKLW
Sbjct:   41 HTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGK--------FEKSLSGHKLGISDVAWSP-----DSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTLIDDDNPPVSFVKFSP---NG-KYILAATLDNTLKLW---DYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHMVYIWNLQTKEIVQKLQGHADVVIATACHPSENIIASAALENDKTIKLW 327

HSP 3 Score: 80.1073 bits (196), Expect = 6.049e-16
Identity = 72/249 (28.92%), Postives = 107/249 (42.97%), Query Frame = 3 
Query: 1011 PHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            P+  W+ S S   D  I+I G+F+  +E           + L    L I + A    +  +V  SDD TL +W  +  +      + GH N V    F+P    + S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W     + L   ID     +SF K   +PN K    + L              L+   GH +    VFA      G  + SG +D +V +W
Sbjct:   52 PNGEWLASSS--ADKLIKIWGSFDGKFE-----------KSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIW--DMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHMVYIW 283
[Close]
BLAST of notchless-1 vs. Ensembl Cavefish
Match: ENSAMXT00000007962.2 (WD repeat-containing protein 5-like [Source:NCBI gene;Acc:103023146])

HSP 1 Score: 100.138 bits (248), Expect = 1.669e-22
Identity = 58/172 (33.72%), Postives = 89/172 (51.74%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N  +   + GH   ++ VKFSP+G ++AS+S D+ +KIW    GKF  SL GH   ++ ++WS DS LLVS S D ++K+W M   + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   27 PVKPNYTLKFTLAGHTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKFEKSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKC---LKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMW 195

HSP 2 Score: 83.9593 bits (206), Expect = 3.953e-17
Identity = 73/271 (26.94%), Postives = 115/271 (42.44%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKL-VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQE 1409
            H   +  + +SPNG+ LASSS    I  W   +GK+        +K+ S  KL +  +AW P        LL +A DDKT+ IWD    +   +L GH   V C  +   SNL+ SG  D  +R+W  + G   +++  HS  ++++  N D ++                                          +V  S D    +W    S + +   +      V+ VKFSP+G +I +A+ D ++K+WD   GK L +  GH  E
Sbjct:   41 HTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKF--------EKSLSGHKLGISDVAWSP-----DSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSL------------------------------------------IVSSSYDGLCRMWD-STSGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNE 255

HSP 3 Score: 75.8702 bits (185), Expect = 2.007e-14
Identity = 59/192 (30.73%), Postives = 87/192 (45.31%), Query Frame = 3 
Query: 1005 MKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCK 1577
              P+  W+ S S   D  I+I G+F+  +E           + L    L I + A    +  +V  SDD TL +W  +  +      + GH N V    F+P    + S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W     + L   ID     +SF K   +PN K
Sbjct:   50 FSPNGEWLASSS--ADKLIKIWGSFDGKFE-----------KSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIW--DMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTLIDDDNPPVSFVK--FSPNGK 224

HSP 4 Score: 58.151 bits (139), Expect = 9.233e-9
Identity = 84/363 (23.14%), Postives = 142/363 (39.12%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDV--KFSPDG------LFIASASFDRSVKIWDGMTGKFLVSLQG-HMQEVNLISWSCDSRLLVSCSQDSSIKLWTM 1490
            V+P      ++ GH KA+   +FSP+G +LA+ SAD  ++ W       E  + + HK  +  ++WSP+  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W +  G   +++                   I   NP     K + N                       + ++  + DNTL LW   + +K  C +  TGH+N    V   FS  G      LF+   +F  S     G T  FLVS+   +   ++ I W      +VS S+D  + +W +
Sbjct:   28 VKPNYTLKFTLAGHTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKFEKSL-SGHKLGISDVAWSPDSNLLVSASDDKTLKIWDMGSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTL-------------------IDDDNPPVSFVKFSPN----------------------GKYILAATLDNTLKLW---DYSKGKCLKTYTGHKNEKYCVFANFSVTGGKVRRLLFLDLVTF--SAAFGRGQTVLFLVSVYSINTSLIDSIQW------IVSGSEDHMVYIWNL 325
[Close]
BLAST of notchless-1 vs. Ensembl Cavefish
Match: ENSAMXT00000052579.1 (WD repeat-containing protein 5-like [Source:NCBI gene;Acc:103023146])

HSP 1 Score: 100.138 bits (248), Expect = 1.669e-22
Identity = 58/172 (33.72%), Postives = 89/172 (51.74%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N  +   + GH   ++ VKFSP+G ++AS+S D+ +KIW    GKF  SL GH   ++ ++WS DS LLVS S D ++K+W M   + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   27 PVKPNYTLKFTLAGHTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKFEKSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKC---LKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMW 195

HSP 2 Score: 83.9593 bits (206), Expect = 3.953e-17
Identity = 73/271 (26.94%), Postives = 115/271 (42.44%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKL-VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQE 1409
            H   +  + +SPNG+ LASSS    I  W   +GK+        +K+ S  KL +  +AW P        LL +A DDKT+ IWD    +   +L GH   V C  +   SNL+ SG  D  +R+W  + G   +++  HS  ++++  N D ++                                          +V  S D    +W    S + +   +      V+ VKFSP+G +I +A+ D ++K+WD   GK L +  GH  E
Sbjct:   41 HTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKF--------EKSLSGHKLGISDVAWSP-----DSNLLVSASDDKTLKIWDMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSL------------------------------------------IVSSSYDGLCRMWD-STSGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNE 255

HSP 3 Score: 75.8702 bits (185), Expect = 2.007e-14
Identity = 59/192 (30.73%), Postives = 87/192 (45.31%), Query Frame = 3 
Query: 1005 MKPHSHWINSISLNTDYAIRI-GAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCK 1577
              P+  W+ S S   D  I+I G+F+  +E           + L    L I + A    +  +V  SDD TL +W  +  +      + GH N V    F+P    + S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W     + L   ID     +SF K   +PN K
Sbjct:   50 FSPNGEWLASSS--ADKLIKIWGSFDGKFE-----------KSLSGHKLGISDVAWSPDSNLLVSASDDKTLKIW--DMGSGKCLKTLKGHSNYVFCCNFNPQSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTLIDDDNPPVSFVK--FSPNGK 224

HSP 4 Score: 58.151 bits (139), Expect = 9.233e-9
Identity = 84/363 (23.14%), Postives = 142/363 (39.12%), Query Frame = 3 
Query:  435 VRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDV--KFSPDG------LFIASASFDRSVKIWDGMTGKFLVSLQG-HMQEVNLISWSCDSRLLVSCSQDSSIKLWTM 1490
            V+P      ++ GH KA+   +FSP+G +LA+ SAD  ++ W       E  + + HK  +  ++WSP+  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W +  G   +++                   I   NP     K + N                       + ++  + DNTL LW   + +K  C +  TGH+N    V   FS  G      LF+   +F  S     G T  FLVS+   +   ++ I W      +VS S+D  + +W +
Sbjct:   28 VKPNYTLKFTLAGHTKAISSVKFSPNGEWLASSSADKLIKIWGSFDGKFEKSL-SGHKLGISDVAWSPDSNLLVSASDDKTLKIWDMGSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLVVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRMWDSTSGQCLKTL-------------------IDDDNPPVSFVKFSPN----------------------GKYILAATLDNTLKLW---DYSKGKCLKTYTGHKNEKYCVFANFSVTGGKVRRLLFLDLVTF--SAAFGRGQTVLFLVSVYSINTSLIDSIQW------IVSGSEDHMVYIWNL 325
[Close]
BLAST of notchless-1 vs. Ensembl Cavefish
Match: wdr5 (WD repeat domain 5 [Source:NCBI gene;Acc:103033424])

HSP 1 Score: 96.6709 bits (239), Expect = 1.679e-21
Identity = 56/172 (32.56%), Postives = 89/172 (51.74%), Query Frame = 3 
Query: 1230 PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            P + N  +   + GH   V+ VKFSP G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DS LLVS S D ++K+W ++  + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   30 PVKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 198

HSP 2 Score: 85.1149 bits (209), Expect = 1.222e-17
Identity = 80/295 (27.12%), Postives = 123/295 (41.69%), Query Frame = 3 
Query:  873 KISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            K +L+GH K+V  +K+  S   + S   D  I++W A DG   +++  H   I+ ++ ++D                   +NL                       +V  SDD TL +W  + S+      + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   38 KFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSD-------------------SNL-----------------------LVSASDDKTLKIW--DVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 286

HSP 3 Score: 70.0922 bits (170), Expect = 1.255e-12
Identity = 80/354 (22.60%), Postives = 137/354 (38.70%), Query Frame = 3 
Query:  348 GVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
               E K  +E    +T             V+P      ++ GH KAV   +FSP G +LA+ SAD  ++ W       E  + + HK  +  ++WS +  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +   K I  ++ GH +VV      P    IASA+   D+++K+W
Sbjct:    2 ATEEKKPETEATKTQTSSAASASQSKSAPVKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--TKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW 330
[Close]
BLAST of notchless-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002506.1 (pep scaffold:Pmarinus_7.0:GL479076:26529:32136:-1 gene:ENSPMAG00000002224.1 transcript:ENSPMAT00000002506.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.5001 bits (210), Expect = 1.943e-18
Identity = 64/197 (32.49%), Postives = 96/197 (48.73%), Query Frame = 3 
Query: 1182 ERMVVCSDDNTLSLWK--PEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDIS---FKKKITAP-----------------NCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + ++  S D TL +WK   +E+N  I  R + GH + V+DV  S DG F  S S+D ++++WD  TG       GH ++V  +++S D+R +VS S+D +IKLW    +    +  D   E +S   F      P                 NCK  + +H+     GH   +  +  SPDG + ASGGKDG   LW
Sbjct:   29 DMILSASRDKTLIMWKLTRDETNYGIPQRSLHGHGHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSADNRQIVSGSRDKTIKLWNTLGVCKYTIQEDGHTEWVSCVRFSPNNNNPIIVSCGWDKLVKVWNLTNCK-LKTNHI-----GHTGYLNTVTVSPDGSLCASGGKDGQAMLW 219

HSP 2 Score: 62.7734 bits (151), Expect = 5.375e-11
Identity = 74/308 (24.03%), Postives = 124/308 (40.26%), Query Frame = 3 
Query:  810 LLAAAYDDKTITIW-----DTILCQPKISLSGHIKSVR-CMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD-YAIRIGAFNPMYE--STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKED-ISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            ++ +A  DKT+ +W     +T    P+ SL GH   V   +         SG  D  +R+W    G   R    H+  + S++ + D   I  G+ +   +  +T         E+     +     +  N    +V C  D  + +W    +N  +     GH   +N V  SPDG   AS   D    +WD   GK L +L G    +N + +S  +R  +  +   SIK+W    + G ++ +D+L+++ IS   K   P C                    +L WS DG  + +G  D  +R+W
Sbjct:   30 MILSASRDKTLIMWKLTRDETNYGIPQRSLHGHGHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSADNRQIVSGSRDKTIKLWNTLGVCKYTIQEDGHTEWVSCVRFSPNNNNPIIVSCGWDKLVKVW--NLTNCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGG-DTINALCFS-PNRYWLCAATGPSIKIW---DLEGKVI-VDELRQEVISTSSKAEPPQC-------------------ISLAWSADGQTLFAGYTDNFIRVW 310
[Close]
BLAST of notchless-1 vs. Ensembl Sea Lamprey
Match: snrnp40 (small nuclear ribonucleoprotein 40 (U5) [Source:ZFIN;Acc:ZDB-GENE-040426-978])

HSP 1 Score: 84.3445 bits (207), Expect = 5.451e-18
Identity = 85/364 (23.35%), Postives = 147/364 (40.38%), Query Frame = 3 
Query:  399 LRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKW---GGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKL 1481
            L  V  P+S   + PI   T    GH+  V   +F P+G  LA+   D T+  W++            H   +L + +S +G  + +SS    +       G W  +T   IK+       V +            +++  A DD T+ +WDT   + K S+     + +C+       S+ I SGG D  I+VW      L  +MK H+  +  +SL+ D +  +   N M  S +  I ++      +R + I+     N  + ++ CS                                +S DG  +++ S DR V IWD  + + L  L GH   VN +++  +  +++SC+ D  + L
Sbjct:   35 LLAVGPPRSSSLLAPIMLLT----GHEGEVYCCKFHPNGNTLASAGFDRTILMWNVYGDCDNYAHLKGHTGAILDIHYSTDGSTIFTSSTDKTV-------GLWDSETGERIKRLKGHTSFVNAC----FPARRGPQMVCTASDDGTVKLWDT---RKKGSVHSFQNTYQCLSVTFNDTSDQILSGGIDNVIKVWDLRQNKLIYTMKGHNDSVTGLSLSPDGSYLLS--NAMDNSVR--IWDIRPFAPMERCVKIFQGNVHNFEKNLLKCS--------------------------------WSHDGSKVSAGSADRFVYIWDTTSRRILYKLPGHAGSVNDVAFHPEEPIVLSCANDKRLYL 344

HSP 2 Score: 64.3142 bits (155), Expect = 2.377e-11
Identity = 73/309 (23.62%), Postives = 129/309 (41.75%), Query Frame = 3 
Query:  813 LAAAYDDKTITIWDTIL-CQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALL--IYNTAR------RNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD----GMTGKFLVSLQG--HMQEVNLI--SWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKD 1685
            LA+A  D+TI +W+    C     L GH  ++  + +    + I++   D  + +W +E G   + +K H+ ++N+      +  R G   P    T ++   +   + +K+  +    NT +       + +++++    DN + +W   + NK I   M GH + V  +  SPDG ++ S + D SV+IWD        + +   QG  H  E NL+  SWS D   + + S D  + +W     R                               +L  LPGHA SV  + + P+  +V S   D
Sbjct:   72 LASAGFDRTILMWNVYGDCDNYAHLKGHTGAILDIHYSTDGSTIFTSSTDKTVGLWDSETGERIKRLKGHTSFVNAC-----FPARRG---PQMVCTASDDGTVKLWDTRKKGSVHSFQNTYQCLSVTFNDTSDQILSGGIDNVIKVWDLRQ-NKLIY-TMKGHNDSVTGLSLSPDGSYLLSNAMDNSVRIWDIRPFAPMERCVKIFQGNVHNFEKNLLKCSWSHDGSKVSAGSADRFVYIWDTTSRR-------------------------------ILYKLPGHAGSVNDVAFHPEEPIVLSCAND 339

HSP 3 Score: 64.3142 bits (155), Expect = 2.508e-11
Identity = 44/160 (27.50%), Postives = 73/160 (45.62%), Query Frame = 3 
Query: 1260 RMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVN-LISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRA-----------RISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
             + GH   + D+ +S DG  I ++S D++V +WD  TG+ +  L+GH   VN         +++ + S D ++KLW   + +G +       + +S     T+                 R + L+  + GH DSV  L  SPDG  + S   D  VR+W
Sbjct:   95 HLKGHTGAILDIHYSTDGSTIFTSSTDKTVGLWDSETGERIKRLKGHTSFVNACFPARRGPQMVCTASDDGTVKLWDTRK-KGSVHSFQNTYQCLSVTFNDTSDQILSGGIDNVIKVWDLRQNKLIYTMKGHNDSVTGLSLSPDGSYLLSNAMDNSVRIW 253

HSP 4 Score: 50.447 bits (119), Expect = 5.506e-7
Identity = 36/119 (30.25%), Postives = 60/119 (50.42%), Query Frame = 3 
Query:  354 NETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTP-EACME----NSH--KSNVLLLSWSPNGKVLASSSVTGEIIPW 689
            N+T +   + GI+ V++       V+ +R   +   +M GH  +V     SPDG YL + + DN+VR WD+    P E C++    N H  + N+L  SWS +G  +++ S    +  W
Sbjct:  193 NDTSDQILSGGIDNVIK-------VWDLRQ-NKLIYTMKGHNDSVTGLSLSPDGSYLLSNAMDNSVRIWDIRPFAPMERCVKIFQGNVHNFEKNLLKCSWSHDGSKVSAGSADRFVYIW 303

HSP 5 Score: 48.521 bits (114), Expect = 2.717e-6
Identity = 26/76 (34.21%), Postives = 42/76 (55.26%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD--GMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            +TGH+  V   KF P+G  +ASA FDR++ +W+  G    +   L+GH   +  I +S D   + + S D ++ LW
Sbjct:   53 LTGHEGEVYCCKFHPNGNTLASAGFDRTILMWNVYGDCDNY-AHLKGHTGAILDIHYSTDGSTIFTSSTDKTVGLW 127
[Close]
BLAST of notchless-1 vs. Ensembl Sea Lamprey
Match: POC1A (POC1 centriolar protein A [Source:HGNC Symbol;Acc:HGNC:24488])

HSP 1 Score: 85.5001 bits (210), Expect = 6.633e-18
Identity = 86/370 (23.24%), Postives = 135/370 (36.49%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG--GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAIC-GRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            H+  +  + ++PN K LAS S+   ++ W+        K      +    K  V S+ + P     +  L+ +A  DKT+ +W   +         H  +VR + +   G +L+ +  +D  I+VW         S+  HS+W+ S   + D                                             +V  SDD T+ +W   +SN   C      H   VN V F P G  IA+A  D +VK+WD    K L   Q H   VN +S+      L++ S DS++K+  + + R                               L   L GH     A+ +S  G   ASGG D  V +W
Sbjct:   17 HRDTITSVDFNPNMKQLASGSMDACLMVWNF-------KPQTRAYRFVGHKDAVMSVQFSP-----SGHLVVSASRDKTVRLWVPSVKGESTVFKAHTATVRSVSFSADGQSLV-TASDDKSIKVWTVHRQKFLYSLNQHSNWVRSAKFSPD------------------------------------------GRLIVSGSDDRTVRVW---DSNSRECVHTFYEHGGFVNQVDFHPSGTCIAAAGTDNTVKVWDIRMNKLLQHYQVHGAAVNSLSFHPTGNYLITASNDSTLKVLDLVEGR-------------------------------LFYTLHGHQGPATAVAFSRAGDFFASGGSDEQVMVW 297

HSP 2 Score: 83.9593 bits (206), Expect = 2.133e-17
Identity = 80/339 (23.60%), Postives = 136/339 (40.12%), Query Frame = 3 
Query:  471 GHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            GH+  +    F+P+ + LA+GS D  +  W+   QT  A     HK  V+ + +SP+G ++ S+S    +  W       S K  + + K  +A   V+S+++     DG  + L  A DDK+I +W     +   SL+ H   VR  K+     LI SG +D  +RVW +       +   H  ++N +            F+P                                   +     DNT+ +W     NK +      H   VN + F P G ++ +AS D ++K+ D + G+   +L GH      +++S       S   D  + +W
Sbjct:   16 GHRDTITSVDFNPNMKQLASGSMDACLMVWNFKPQT-RAYRFVGHKDAVMSVQFSPSGHLVVSASRDKTVRLWVP-----SVKGESTVFKAHTAT--VRSVSFS---ADG--QSLVTASDDKSIKVWTVHRQKFLYSLNQHSNWVRSAKFSPDGRLIVSGSDDRTVRVWDSNSRECVHTFYEHGGFVNQVD-----------FHP-------------------------------SGTCIAAAGTDNTVKVWD-IRMNK-LLQHYQVHGAAVNSLSFHPTGNYLITASNDSTLKVLDLVEGRLFYTLHGHQGPATAVAFSRAGDFFASGGSDEQVMVW 297

HSP 3 Score: 73.9442 bits (180), Expect = 2.969e-14
Identity = 78/312 (25.00%), Postives = 128/312 (41.03%), Query Frame = 3 
Query:  426 VFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNL-IYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIW 1358
            V+  +P TR    + GHK AV+  QFSP G  + + S D TVR W + +   E+ +  +H + V  +S+S +G+ L ++S    I  W++   K+      L   N  +   V+S  + P   DG  RL+ +  DD+T+ +WD+   +   +   H   V  + +  S   I + G D  ++VW      L +  + H   +NS+S           F+P                                   ++  S+D+TL +    E    +   + GHQ     V FS  G F AS   D  V +W
Sbjct:   44 VWNFKPQTRAYRFV-GHKDAVMSVQFSPSGHLVVSASRDKTVRLW-VPSVKGESTVFKAHTATVRSVSFSADGQSLVTASDDKSIKVWTVHRQKF------LYSLNQHS-NWVRSAKFSP---DG--RLIVSGSDDRTVRVWDSNSRECVHTFYEHGGFVNQVDFHPSGTCIAAAGTDNTVKVWDIRMNKLLQHYQVHGAAVNSLS-----------FHPT-------------------------------GNYLITASNDSTLKVLDLVEGR--LFYTLHGHQGPATAVAFSRAGDFFASGGSDEQVMVW 297

HSP 4 Score: 67.3958 bits (163), Expect = 3.606e-12
Identity = 64/251 (25.50%), Postives = 94/251 (37.45%), Query Frame = 3 
Query:  981 EDGVLCRSMKPHSHWINSISLNT----------DYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            ED  L R  K H   I S+  N           D  + +  F P   + +  + + DA         + +         +V  S D T+ LW P  S K        H   V  V FS DG  + +AS D+S+K+W     KFL SL  H   V    +S D RL+VS S D ++++W                            +  R  +    E    H   V  +D+ P G  +A+ G D  V++W
Sbjct:    6 EDPSLERHFKGHRDTITSVDFNPNMKQLASGSMDACLMVWNFKPQTRAYRF-VGHKDA---------VMSVQFSPSGHLVVSASRDKTVRLWVP--SVKGESTVFKAHTATVRSVSFSADGQSLVTASDDKSIKVWTVHRQKFLYSLNQHSNWVRSAKFSPDGRLIVSGSDDRTVRVWD---------------------------SNSRECVHTFYE----HGGFVNQVDFHPSGTCIAAAGTDNTVKVW 213

HSP 5 Score: 64.3142 bits (155), Expect = 3.559e-11
Identity = 54/224 (24.11%), Postives = 92/224 (41.07%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAE-------------------------DGVLCRSMKPHSHWINSISLNT 1049
            S+  H   V  A+FSPDGR + +GS D TVR WD N++    C+     H   V  + + P+G  +A++     +  W +       + + L++        V SL++ P     T   L  A +D T+ + D +  +   +L GH      + +    +   SGG D ++ VW+                             +  RS +PHS   NSI +++
Sbjct:  139 SLNQHSNWVRSAKFSPDGRLIVSGSDDRTVRVWDSNSR---ECVHTFYEHGGFVNQVDFHPSGTCIAAAGTDNTVKVWDI-------RMNKLLQHYQVHGAAVNSLSFHP-----TGNYLITASNDSTLKVLDLVEGRLFYTLHGHQGPATAVAFSRAGDFFASGGSDEQVMVWKTNFDSVDYSEVLRSHKQRANPDPPPYVHDIPPRSPRPHSQSANSIQIHS 347
[Close]
BLAST of notchless-1 vs. Ensembl Sea Lamprey
Match: taf5l (TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor [Source:ZFIN;Acc:ZDB-GENE-030131-5987])

HSP 1 Score: 85.1149 bits (209), Expect = 1.458e-17
Identity = 52/169 (30.77%), Postives = 78/169 (46.15%), Query Frame = 3 
Query: 1200 SDDNTLSLWKPEESNKAICGRM-TGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            S D T  LW PE   +A   R+  GH + VN V+F P+G ++A+ S DR+V++W    G       GH   V  +++S   RLL S  +D  ++LW +                                   +++ L GH+D +  L +SPDG  +ASG  DG VR+W
Sbjct:  404 SHDRTARLWVPE---RAFPLRVYAGHVSDVNVVRFHPNGSYVATGSADRTVRLWSAQQGSAARLFTGHRGAVLALAFSPGGRLLASGGEDQRVRLWDIGS-------------------------------GAIVKELRGHSDDITCLSFSPDGSALASGSSDGSVRVW 538

HSP 2 Score: 69.707 bits (169), Expect = 8.207e-13
Identity = 43/129 (33.33%), Postives = 66/129 (51.16%), Query Frame = 3 
Query:  471 GHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDT 857
            GH   V V +F P+G Y+ATGSAD TVR W    Q   A +   H+  VL L++SP G++LAS      +  W + +G       A++K+       +  L++ P   DG+   LA+   D ++ +WD 
Sbjct:  425 GHVSDVNVVRFHPNGSYVATGSADRTVRLWSAQ-QGSAARLFTGHRGAVLALAFSPGGRLLASGGEDQRVRLWDIGSG-------AIVKELRGHSDDITCLSFSP---DGSA--LASGSSDGSVRVWDV 540

HSP 3 Score: 58.9214 bits (141), Expect = 2.351e-9
Identity = 51/175 (29.14%), Postives = 76/175 (43.43%), Query Frame = 3 
Query:  456 TSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG--GSNLIYSGGEDCEIRVW 974
            T+   GH   V     S  G Y  +GS D T R W      P       H S+V ++ + PNG  +A+ S    +  WS      +Q+ SA  +  +  +  V +LA+ P       RLLA+  +D+ + +WD         L GH   + C+ +   GS L  SG  D  +RVW
Sbjct:  378 TAVYQGHAYPVWDVDVSALGLYFCSGSHDRTARLWVPERAFPLRVYAG-HVSDVNVVRFHPNGSYVATGSADRTVRLWS------AQQGSA-ARLFTGHRGAVLALAFSP-----GGRLLASGGEDQRVRLWDIGSGAIVKELRGHSDDITCLSFSPDGSALA-SGSSDGSVRVW 538

HSP 4 Score: 49.6766 bits (117), Expect = 1.666e-6
Identity = 39/178 (21.91%), Postives = 68/178 (38.20%), Query Frame = 3 
Query:  831 DKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD 1361
            D+T  +W      P    +GH+  V  +++    + + +G  D  +R+W A+ G   R    H   + ++           AF+P                               G   +    +D  + LW  +  + AI   + GH + +  + FSPDG  +AS S D SV++WD
Sbjct:  406 DRTARLWVPERAFPLRVYAGHVSDVNVVRFHPNGSYVATGSADRTVRLWSAQQGSAARLFTGHRGAVLAL-----------AFSP-------------------------------GGRLLASGGEDQRVRLW--DIGSGAIVKELRGHSDDITCLSFSPDGSALASGSSDGSVRVWD 539
[Close]
BLAST of notchless-1 vs. Ensembl Sea Lamprey
Match: ciao1 (cytosolic iron-sulfur assembly component 1 [Source:NCBI gene;Acc:795104])

HSP 1 Score: 82.8037 bits (203), Expect = 1.680e-17
Identity = 94/378 (24.87%), Postives = 158/378 (41.80%), Query Frame = 3 
Query:  465 MPGHKKAVLVAQFSPDGRYLATGSADNTVRFW--DLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDT-----ILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESN--------------KAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIW--------DGMTGKFLVSL---QGHMQEVNLISWS-CDSRLLVSCSQDSSIKLWTMAQ 1496
            + GH+       +SP G  +A+   D TVR W  + +     + + ++H+  V  ++WSP G  LAS+S       WS  +G +    +    +N      VK +AW P     +  LLA    DK++ IW+        C     L+ H + V+ + W   S ++ S   D  IR++R EDG           W +  +L              +EST  +I+  DA            T RR     +V C DD T+ +W+   +               + +C     H  V+ DV +      IA+AS D +++++        DG + +F  ++   + H ++VN ++WS  D  LL SC  D  + +W  A+
Sbjct:   11 LAGHQDRAWSVAWSPSGALIASCGGDKTVRVWGREGDGWVCRSVLADAHQRTVRNVAWSPCGTRLASASFDATTCVWSRRDGDFEVTATLEGHENE-----VKGVAWAP-----SGNLLATCSRDKSVWIWEVDEEEDYECASV--LNSHTQDVKHVVWHPNSEVLASASYDNSIRLYR-EDG---------DDWSSFATLEG------------HESTVWSID-FDA------------TGRR-----LVSCGDDKTIRIWQEGAATSSGGCICVAVGATWECVCTLSGHHTRVIYDVSWCKLTGAIATASGDDTIRVFEEEAVPEGDGKSPRFYEAVCVRKAHDRDVNCVAWSPSDPGLLASCGDDRVVAIWRYAR 336

HSP 2 Score: 60.4622 bits (145), Expect = 3.252e-10
Identity = 43/134 (32.09%), Postives = 66/134 (49.25%), Query Frame = 3 
Query:  456 TSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACME--NSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIW 851
            T+++ GH+  V    ++P G  LAT S D +V  W+++ +    C    NSH  +V  + W PN +VLAS+S    I  +      WS  + A ++ +       +S  W  +  D T R L +  DDKTI IW
Sbjct:   97 TATLEGHENEVKGVAWAPSGNLLATCSRDKSVWIWEVDEEEDYECASVLNSHTQDVKHVVWHPNSEVLASASYDNSIRLYREDGDDWS--SFATLEGH-------ESTVWS-IDFDATGRRLVSCGDDKTIRIW 220

HSP 3 Score: 53.9138 bits (128), Expect = 5.249e-8
Identity = 41/153 (26.80%), Postives = 70/153 (45.75%), Query Frame = 3 
Query: 1260 RMTGHQNVVNDVKFSPDGLFIASASFDRSVKIW----DGMTGKFLVSLQGHMQEVNLISWS-CDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            R+ GHQ+    V +SP G  IAS   D++V++W    DG   + +++   H + V  ++WS C +R L S S D++  +W+              + D  F+   T               L GH + V  + W+P G ++A+  +D  V +W
Sbjct:   10 RLAGHQDRAWSVAWSPSGALIASCGGDKTVRVWGREGDGWVCRSVLA-DAHQRTVRNVAWSPCGTR-LASASFDATTCVWS--------------RRDGDFEVTAT---------------LEGHENEVKGVAWAPSGNLLATCSRDKSVWIW 131

HSP 4 Score: 52.7582 bits (125), Expect = 1.230e-7
Identity = 78/389 (20.05%), Postives = 153/389 (39.33%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKIS--LSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRA--EDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD---------------GMTGKFLVSLQGHMQEVNL-ISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSP-DGMVVASGGKDGLVRLW 1703
            H+     ++WSP+G ++AS      +  W      W  + S L   +   ++ V+++AW P    GT RL +A++D  T  +W       +++  L GH   V+ + W  S NL+ +   D  + +W    E+   C S+      +NS + +  + +    ++P                                +E +   S DN++ L++ +  + +    + GH++ V  + F   G  + S   D++++IW                G T + + +L GH   V   +SW   +  + + S D +I+++                     ++ +   + K  R    +     H   V  + WSP D  ++AS G D +V +W
Sbjct:   14 HQDRAWSVAWSPSGALIASCGGDKTVRVWGREGDGWVCR-SVLADAH---QRTVRNVAWSPC---GT-RLASASFD-ATTCVWSRRDGDFEVTATLEGHENEVKGVAWAPSGNLLATCSRDKSVWIWEVDEEEDYECASV------LNSHTQDVKHVV----WHP-------------------------------NSEVLASASYDNSIRLYREDGDDWSSFATLEGHESTVWSIDFDATGRRLVSCGDDKTIRIWQEGAATSSGGCICVAVGATWECVCTLSGHHTRVIYDVSWCKLTGAIATASGDDTIRVFE--------------------EEAVPEGDGKSPRFYEAVCVRKAHDRDVNCVAWSPSDPGLLASCGDDRVVAIW 332
[Close]
BLAST of notchless-1 vs. Ensembl Yeast
Match: RSA4 (WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus [Source:SGD;Acc:S000000668])

HSP 1 Score: 332.413 bits (851), Expect = 9.181e-108
Identity = 207/546 (37.91%), Postives = 301/546 (55.13%), Query Frame = 3 
Query:   93 IKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPD-GRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHL--DGTCRLLAAAYDDKTITIWDTI--LCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRS-MKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNT-ARRNG--AERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            IK Q +D+ G N G   R+P + + K L   +N+ L     +   Y+F  T  ++ +K      + + T      +    +K  Y   E +           + ++Y P++VF+V+P+TR +S++ GH   +L + F+P     + TG+ DNT R WD +TQTP   ++  H + VL +SWSP+G+V+A+ S+   I  W   +G   Q     ++ +S   K + SL+W+P+HL   G+   LA++  D TI IWDT+  +CQ   ++SGH  SV C+KWGG  L+YSG  D  +RVW       C + +K H+HW+N +SL+TDYA+RIGAF+   +   T       EE QK+AL  Y    ++NG   E MV  SDD T+ LW P +S K I  RMTGHQ +VN V FSPDG +I SASFD S+K+WDG  GKF+ + +GH+  V  ++WS D RLLVSCS+D+++K+W +                               R   L   LPGH D V+ +DWS DG  V SGGKD +VRLW
Sbjct:   34 IKFQALDT-GDNVGGALRVPGAISEKQLEELLNQ-LNGTSDDPVPYTFSCT--IQGKK----ASDPVKTIDITDNLYSSLIKPGYNSTEDQ-----------ITLLYTPRAVFKVKPVTRSSSAIAGHGSTILCSAFAPHTSSRMVTGAGDNTARIWDCDTQTPMHTLKG-HYNWVLCVSWSPDGEVIATGSMDNTIRLWDPKSG---QCLGDALRGHS---KWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIWDTVSRVCQ--YTMSGHTNSVSCVKWGGQGLLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLSLSTDYALRIGAFDHTGKKPST------PEEAQKKALENYEKICKKNGNSEEMMVTASDDYTMFLWNPLKSTKPI-ARMTGHQKLVNHVAFSPDGRYIVSASFDNSIKLWDGRDGKFISTFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDV-------------------------------RTRKLSVDLPGHKDEVYTVDWSVDGKRVCSGGKDKMVRLW 513
[Close]
BLAST of notchless-1 vs. Ensembl Yeast
Match: PRP4 (Splicing factor; component of the U4/U6-U5 snRNP complex [Source:SGD;Acc:S000006382])

HSP 1 Score: 89.7373 bits (221), Expect = 1.716e-19
Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 3 
Query: 1185 RMVVCSDDNTLS--LWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +M+ C++D  +    +  EE    + G + GH+  ++DVK+ P G FI SAS D + ++WD  T + L+  +GH + V  +S+ CD  L+ S   DS   LW                 DI    K+                L GH+  ++ + WSP+G  VA+GG DG++ +W
Sbjct:  233 QMISCAEDGLIKNFQYSNEEGGLRLLGDLVGHERRISDVKYHPSGKFIGSASHDMTWRLWDASTHQELLLQEGHDKGVFSLSFQCDGSLVCSGGMDSLSMLW-----------------DIRSGSKVM--------------TLAGHSKPIYTVAWSPNGYQVATGGGDGIINVW 376

HSP 2 Score: 60.4622 bits (145), Expect = 3.909e-10
Identity = 60/301 (19.93%), Postives = 127/301 (42.19%), Query Frame = 3 
Query:  480 KAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSAL--IKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD-YAIRIGAFNPM---YESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD 1361
            K +     S D   +ATGS    ++  +  T  P     +SH   +  + W P+      S     +I     N ++S +   L  +      ++ +  + + P     + + + +A  D T  +WD    Q  +   GH K V  + +    +L+ SGG D    +W    G    ++  HS  I +++ + + Y +  G  + +   ++  K +   L+     +  +     ++ +G +++V C  DN ++++  +   K   G + GH + +  +  S +  F+ S  +DRS+K+W+
Sbjct:  176 KPISAVSLSTDDMVVATGSWAGDLQVLNSQTLQPLTQKLDSHVGKIGAIDWHPDSNNQMISCAEDGLIK----NFQYSNEEGGLRLLGDLVGHERRISDVKYHP-----SGKFIGSASHDMTWRLWDASTHQELLLQEGHDKGVFSLSFQCDGSLVCSGGMDSLSMLWDIRSGSKVMTLAGHSKPIYTVAWSPNGYQVATGGGDGIINVWDIRKRDEGQLNQILAHRNIVTQVRFSKEDGGKKLVSCGYDNLINVYSSDTWLK--MGSLAGHTDKIISLDISNNSHFLVSGGWDRSIKLWN 465

HSP 3 Score: 52.7582 bits (125), Expect = 1.071e-7
Identity = 48/211 (22.75%), Postives = 87/211 (41.23%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWD----------TILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD 1052
            R    + GH++ +   ++ P G+++ + S D T R WD +T   E  ++  H   V  LS+  +G ++ S  +    + W + +G    K   L    +   K + ++AW P         +A    D  I +WD           IL    I     +  VR  K  G   + S G D  I V+ ++  +   S+  H+  I S+ ++ +
Sbjct:  256 RLLGDLVGHERRISDVKYHPSGKFIGSASHDMTWRLWDASTHQ-ELLLQEGHDKGVFSLSFQCDGSLVCSGGMDSLSMLWDIRSGS---KVMTL----AGHSKPIYTVAWSP-----NGYQVATGGGDGIINVWDIRKRDEGQLNQILAHRNI-----VTQVRFSKEDGGKKLVSCGYDNLINVYSSDTWLKMGSLAGHTDKIISLDISNN 448
[Close]
BLAST of notchless-1 vs. Ensembl Yeast
Match: ASC1 (G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; required to prevent frameshifting at ribosomes stalled at repeated CGA codons; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation [Source:SGD;Acc:S000004722])

HSP 1 Score: 78.5666 bits (192), Expect = 2.182e-16
Identity = 57/195 (29.23%), Postives = 97/195 (49.74%), Query Frame = 3 
Query: 1188 MVVCSDDNTLSLWKPEESNKAI---CGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTM---------------AQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL----LEA-LPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            ++  S D TL  WK    ++          GH ++V D   + DG +  SAS+D+++++WD  TG+      GH  +V  +     + +++S S+D +IK+WT+               +Q+R  +VP ++  +D      I+A N K  +  +L    +EA   GH  ++  L  SPDG ++AS GKDG + LW
Sbjct:   33 LLSASRDKTLISWKLTGDDQKFGVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFVGHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWVSQVR--VVPNEKADDDSV--TIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDGEIMLW 223

HSP 2 Score: 62.003 bits (149), Expect = 6.325e-11
Identity = 69/302 (22.85%), Postives = 120/302 (39.74%), Query Frame = 3 
Query:  921 GGSNLIYSGGEDCEIRVWR--AED---GVLCRSMKPHSHWINSISLNTDYAIRIGA------------FNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAIC-GRMTGHQNVVNDVKFSP------DGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQ-------LKEDISFKKKITAPNCK------RARISHLLEALPGHADS----VFALDWSPDGMVVASGGKDGLVRLW 1703
            G  NL+ S   D  +  W+   +D   GV  RS K HSH +   +L  D A  + A                Y+    + +++ + ++ K+A +I + +R            D T+ +W    + K  C   + GH + V+ V+  P      D + I SA  D+ VK W+    +      GH   +N ++ S D  L+ S  +D  I LW +A  + +     Q          +  +    TA   K      +  +  L     G++ +      +L WS DG  + +G  D ++R+W
Sbjct:   28 GQPNLLLSASRDKTLISWKLTGDDQKFGVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFVGHKSDVMSVDIDKKASMIISGSR------------DKTIKVW----TIKGQCLATLLGHNDWVSQVRVVPNEKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAAATATGIKVFSLDPQYLVDDLRPEFAGYSKAAEPHAVSLAWSADGQTLFAGYTDNVIRVW 313

HSP 3 Score: 49.6766 bits (117), Expect = 5.644e-7
Identity = 55/224 (24.55%), Postives = 94/224 (41.96%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNT-QTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNS--SAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR-----------AEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMY 1088
            S  GH   V     + DG Y  + S D T+R WD+ T +T +  +   HKS+V+ +       ++ S S    I  W+ I G   Q  + L+  N   S  ++V +        D     + +A +DK +  W+    Q +    GH  ++  +       LI S G+D EI +W            A+D V   +  P+ +W+ +    T   I++ + +P Y
Sbjct:   60 SFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQRFV--GHKSDVMSVDIDKKASMIISGSRDKTIKVWT-IKG---QCLATLLGHNDWVSQVRVVPN-----EKADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDGEIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAAA---TATGIKVFSLDPQY 269
[Close]
BLAST of notchless-1 vs. Ensembl Yeast
Match: CDC4 (F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p [Source:SGD;Acc:S000001885])

HSP 1 Score: 76.2554 bits (186), Expect = 6.440e-15
Identity = 77/292 (26.37%), Postives = 122/292 (41.78%), Query Frame = 3 
Query:  828 DDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            DDK I ++D+I  +  + LSGH   V  +K+    ++ SG  D  +RVW  + G      K H+  +  + +  +Y       N  Y  T +  N L   +L K +     +   +G E       D  L    PEE N    G + GH   V  V  S  G  + S S+D ++ +WD    K L  L GH   +    +  + +  +S S D++I++W +  I            + S+     +P C  A+I   +  L GH   V  L  S   +V A+   DG +R W
Sbjct:  400 DDKMIRVYDSINKKFLLQLSGHDGGVWALKYAHGGILVSGSTDRTVRVWDIKKGCCTHVFKGHNSTVRCLDI-VEYK------NIKYIVTGSRDNTLHVWKLPKES-----SVPDHGEEH------DYPLVFHTPEE-NPYFVGVLRGHMASVRTV--SGHGNIVVSGSYDNTLIVWDVAQMKCLYILSGHTDRIYSTIYDHERKRCISASMDTTIRIWDLENIWN--------NGECSYATNSASP-C--AKILGAMYTLQGHTALVGLLRLSDKFLVSAAA--DGSIRGW 657
[Close]
BLAST of notchless-1 vs. Ensembl Yeast
Match: TUP1 (General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes [Source:SGD;Acc:S000000680])

HSP 1 Score: 70.8626 bits (172), Expect = 2.558e-13
Identity = 48/158 (30.38%), Postives = 79/158 (50.00%), Query Frame = 3 
Query: 1284 VNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIR-GLLVPIDQLKEDISFK----KKITAPNCKRA-------------RISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +  V FSPDG F+A+ + DR ++IWD    K ++ LQGH Q++  + +      LVS S D ++++W +   +  L + I+     ++      K I A +  RA             R+    E+  GH DSV+++ ++ DG  V SG  D  V+LW
Sbjct:  446 IRSVCFSPDGKFLATGAEDRLIRIWDIENRKIVMILQGHEQDIYSLDYFPSGDKLVSGSGDRTVRIWDLRTGQCSLTLSIEDGVTTVAVSPGDGKYIAAGSLDRAVRVWDSETGFLVERLDSENESGTGHKDSVYSVVFTRDGQSVVSGSLDRSVKLW 603

HSP 2 Score: 58.5362 bits (140), Expect = 2.226e-9
Identity = 49/187 (26.20%), Postives = 85/187 (45.45%), Query Frame = 3 
Query:  456 TSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDT-------ILCQPKISLSGHIKSVRCMKW--GGSNLIYSGGEDCEIRVWRAEDG 989
            TSS P     +    FSPDG++LATG+ D  +R WD+  +     ++  H+ ++  L + P+G  L S S    +  W L  G+ S           S +  V ++A  P    G  + +AA   D+ + +WD+        L     S +GH  SV  + +   G +++ SG  D  +++W  ++ 
Sbjct:  436 TSSSPSSDLYIRSVCFSPDGKFLATGAEDRLIRIWDIENRKIVMILQ-GHEQDIYSLDYFPSGDKLVSGSGDRTVRIWDLRTGQCSLTL--------SIEDGVTTVAVSP----GDGKYIAAGSLDRAVRVWDSETGFLVERLDSENESGTGHKDSVYSVVFTRDGQSVV-SGSLDRSVKLWNLQNA 608

HSP 3 Score: 57.7658 bits (138), Expect = 3.701e-9
Identity = 69/283 (24.38%), Postives = 117/283 (41.34%), Query Frame = 3 
Query:  747 SAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKW--GGSNLIYSGGEDCEIRVW-----------RAEDGVLCRSMKP-HSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALL--IYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMT----------GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRL------LVSCSQDSSIKLWTMAQI 1499
            S+   ++S+ + P   DG  + LA   +D+ I IWD    +  + L GH + +  + +   G  L+ SG  D  +R+W             EDGV   ++ P    +I + SL  D A+R+         T   +  LD+E          +Y+       + +V  S D ++ LW  + +N     +            GH++ V  V  + +  +I S S DR V  WD  +G  L+ LQGH   V  ++ +  S L        + S D   ++W   +I
Sbjct:  441 SSDLYIRSVCFSP---DG--KFLATGAEDRLIRIWDIENRKIVMILQGHEQDIYSLDYFPSGDKLV-SGSGDRTVRIWDLRTGQCSLTLSIEDGVTTVAVSPGDGKYIAAGSL--DRAVRV-----WDSETGFLVERLDSENESGTGHKDSVYSVVFTRDGQSVVSGSLDRSVKLWNLQNANNKSDSKTPNSGTCEVTYIGHKDFVLSVATTQNDEYILSGSKDRGVLFWDKKSGNPLLMLQGHRNSVISVAVANGSPLGPEYNVFATGSGDCKARIWKYKKI 710

HSP 4 Score: 48.9062 bits (115), Expect = 2.184e-6
Identity = 45/193 (23.32%), Postives = 75/193 (38.86%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQ------TPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTC----RLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-------NLIYSGGEDCEIRVWR 977
            +C+ ++        VA    DG+Y+A GS D  VR WD  T         E      HK +V  + ++ +G+ + S S+   +  W+L N      +    K  +S    V  +  K   L          + +   D+ +  WD     P + L GH  SV  +            N+  +G  DC+ R+W+
Sbjct:  518 QCSLTLSIEDGVTTVAVSPGDGKYIAAGSLDRAVRVWDSETGFLVERLDSENESGTGHKDSVYSVVFTRDGQSVVSGSLDRSVKLWNLQNANNKSDS----KTPNSGTCEVTYIGHKDFVLSVATTQNDEYILSGSKDRGVLFWDKKSGNPLLMLQGHRNSVISVAVANGSPLGPEYNVFATGSGDCKARIWK 706
[Close]
BLAST of notchless-1 vs. Ensembl Nematostella
Match: EDO31555 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXK1])

HSP 1 Score: 413.69 bits (1062), Expect = 1.730e-139
Identity = 228/536 (42.54%), Postives = 310/536 (57.84%), Query Frame = 3 
Query:   93 IKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRL 1700
            I  QF   DG   G  F +P       L    N +L+KE  E   YSFF+        NE    + ++ ++++++                 + ++   E V+ IVY PQ+VF+VR +TRCTSS+PGH +AV+   FSPDGRYLA+GS D TVRFWD+ T+TP    +  H   +L ++WSP+GK LAS    GEI  W    GK   KT   +K +    K +  L+W+PLH +  CR LA++  D ++ IWDT       + S H +SV C+KWGG  LIYS  +D  I+VWRAEDGVLCR+++ H+HW+N ++LNTDY +R GAF P   S  T +  + AE LQ  A   Y  A+ + AER+V  SDD TL LW+PE   K I  RMTGHQ ++N V FSPDG  IASA+FD+SVK+W+G TGKF+ SL+GH+  V  I+WS D RL+ S S DS++K+W M                               +   LL  LPGHAD V+++DWSPDG  V SGGKD ++++
Sbjct:    2 ILAQFKSEDGELVGAPFDLPVDITPDKLELVCNAVLQKE--ETVPYSFFV--------NE----SEVIGSLQDTL-----------------HKQDVETEKVVEIVYQPQAVFKVRAVTRCTSSIPGHTEAVISVAFSPDGRYLASGSGDTTVRFWDVTTETPHFTCKG-HMHWILHIAWSPDGKKLASGCKNGEIRIWDPATGKQMGKT---LKGH---LKWITWLSWEPLHRNPDCRYLASSSKDSSVKIWDTATGSVDKTFSSHTQSVTCVKWGGEGLIYSASQDRTIKVWRAEDGVLCRTLQGHAHWVNHMALNTDYVLRTGAFEP---SKGTTLQTMSAETLQSEASKRYKEAKGSKAERLVSGSDDFTLFLWEPEAKTKPI-ARMTGHQALINQVCFSPDGRLIASAAFDKSVKLWNGETGKFITSLRGHVNCVYQIAWSADCRLICSGSADSTLKVWDM-------------------------------KTKKLLYDLPGHADEVYSVDWSPDGARVGSGGKDKVLKM 464

HSP 2 Score: 71.633 bits (174), Expect = 3.260e-13
Identity = 83/370 (22.43%), Postives = 142/370 (38.38%), Query Frame = 3 
Query:  762 VKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDG-VLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRL---------------------------------------LVSCSQDSSIKLWT-------MAQIRGLLVPIDQ--------LKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V S+A+ P   DG  R LA+   D T+  WD     P  +  GH+  +  + W      + SG ++ EIR+W    G  + +++K H  WI  +S           + P++                          R      +   S D+++ +W  + +  ++    + H   V  VK+  +GL I SAS DR++K+W    G    +LQGH   VN ++ + D  L                                       LVS S D ++ LW        +A++ G    I+Q        L    +F K +   N +  +    + +L GH + V+ + WS D  ++ SG  D  +++W
Sbjct:  102 VISVAFSP---DG--RYLASGSGDTTVRFWDVTTETPHFTCKGHMHWILHIAWSPDGKKLASGCKNGEIRIWDPATGKQMGKTLKGHLKWITWLS-----------WEPLH--------------------------RNPDCRYLASSSKDSSVKIW--DTATGSVDKTFSSHTQSVTCVKWGGEGL-IYSASQDRTIKVWRAEDGVLCRTLQGHAHWVNHMALNTDYVLRTGAFEPSKGTTLQTMSAETLQSEASKRYKEAKGSKAERLVSGSDDFTLFLWEPEAKTKPIARMTGHQALINQVCFSPDGRLIASAAFDKSVKLWNGETGK---FITSLRGHVNCVYQIAWSADCRLICSGSADSTLKVW 423

HSP 3 Score: 61.6178 bits (148), Expect = 4.512e-10
Identity = 42/152 (27.63%), Postives = 64/152 (42.11%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDW-----SPDGMVVASGGKDGLVRLW 1703
            + GH   V  V FSPDG ++AS S D +V+ WD  T     + +GHM  +  I+WS D + L S  ++  I++W  A  +                               + + L GH   +  L W     +PD   +AS  KD  V++W
Sbjct:   95 IPGHTEAVISVAFSPDGRYLASGSGDTTVRFWDVTTETPHFTCKGHMHWILHIAWSPDGKKLASGCKNGEIRIWDPATGK------------------------------QMGKTLKGHLKWITWLSWEPLHRNPDCRYLASSSKDSSVKIW 216
[Close]
BLAST of notchless-1 vs. Ensembl Nematostella
Match: EDO34865 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN68])

HSP 1 Score: 107.457 bits (267), Expect = 4.651e-25
Identity = 76/309 (24.60%), Postives = 147/309 (47.57%), Query Frame = 3 
Query:  831 DKTITIWDTILCQPKISLSGHIKSVRCMKWGG--SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLN-TDYAIRIGAFNPMYE----STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT------MAQIRGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            D+T  +WDT   +  ++L GH   V  + +     + I +G  D   ++W AE G    + + HS  I  ++ N     I  G+ +   +     T   I+ L     +     I + A  +   +++  S D+T+S+W      +     + GH+  +++ +F+ D  FI + S D++ KIWD  TGK + +L+GH  EV  +++    +L+ + S D + +++       ++++ G    I ++  +    + +TA + K AR+        L+ L GH D +F+  ++ DG ++ +G KD   RLW
Sbjct:  113 DRTCKVWDTASGEETLTLEGHKNVVYAIAFNNPYGDKIATGSFDKTCKLWNAETGKCYHTYRGHSAEIVCLAFNPQSTVIATGSMDTTAKLWDVQTGAEISTLSGHSAE-----IISCAFNSTGTQLLTGSFDHTVSVWDTRSGRRV--HTLIGHRGEISNAQFNFDCSFIVTGSMDKTCKIWDAGTGKCIGTLRGHDDEVLDVAFDYTGQLIATASADGTGRVYNALTHHCLSKLEGHEGEISKITFNAQGTRLLTASSDKTARLWDPNTGKCLQVLEGHTDEIFSCAFNYDGDIIITGSKDNTCRLW 414

HSP 2 Score: 87.4261 bits (215), Expect = 2.195e-18
Identity = 80/347 (23.05%), Postives = 151/347 (43.52%), Query Frame = 3 
Query:  456 TSSMPGHKKAVLVAQFS-PDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            T ++ GHK  V    F+ P G  +ATGS D T + W+  T     C      H + ++ L+++P   V+A+ S+      W +       +T A I   S     + S A+      GT +LL  ++ D T+++WDT   +   +L GH   +   ++    + I +G  D   ++W A  G    +++ H   +  ++   DY  ++ A                             TA  +G  R+      + LS             ++ GH+  ++ + F+  G  + +AS D++ ++WD  TGK L  L+GH  E+   +++ D  ++++ S+D++ +LW
Sbjct:  127 TLTLEGHKNVVYAIAFNNPYGDKIATGSFDKTCKLWNAETG---KCYHTYRGHSAEIVCLAFNPQSTVIATGSMDTTAKLWDV-------QTGAEISTLSGHSAEIISCAFNST---GT-QLLTGSF-DHTVSVWDTRSGRRVHTLIGHRGEISNAQFNFDCSFIVTGSMDKTCKIWDAGTGKCIGTLRGHDDEVLDVAF--DYTGQLIA-----------------------------TASADGTGRVYNALTHHCLS-------------KLEGHEGEISKITFNAQGTRLLTASSDKTARLWDPNTGKCLQVLEGHTDEIFSCAFNYDGDIIITGSKDNTCRLW 414

HSP 3 Score: 63.5438 bits (153), Expect = 1.100e-10
Identity = 48/207 (23.19%), Postives = 90/207 (43.48%), Query Frame = 3 
Query: 1131 LQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFS-PDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDI---------------SFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            L+   L + N A        +  S D T  +W      + +   + GH+NVV  + F+ P G  IA+ SFD++ K+W+  TGK   + +GH  E+  ++++  S ++ + S D++ KLW + Q    +  +     +I               SF   ++  + +  R  H    L GH   +    ++ D   + +G  D   ++W
Sbjct:   88 LRAHILPLTNVAFNKSGTSFITGSYDRTCKVWDTASGEETL--TLEGHKNVVYAIAFNNPYGDKIATGSFDKTCKLWNAETGKCYHTYRGHSAEIVCLAFNPQSTVIATGSMDTTAKLWDV-QTGAEISTLSGHSAEIISCAFNSTGTQLLTGSFDHTVSVWDTRSGRRVH---TLIGHRGEISNAQFNFDCSFIVTGSMDKTCKIW 288

HSP 4 Score: 60.4622 bits (145), Expect = 1.058e-9
Identity = 63/305 (20.66%), Postives = 109/305 (35.74%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGS-NLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIW 1358
            +C  +  GH   ++   F+P    +ATGS D T + WD+ T   E    + H + ++  +++  G  L + S    +  W   +G+   +   LI            ++    + D  C  +     DKT  IWD    +   +L GH   V  + +  +  LI +   D   RV+ A        ++ H   I+ I+ N                                              R++  S D T  LW P   N   C + + GH + +    F+ DG  I + S D + ++W
Sbjct:  168 KCYHTYRGHSAEIVCLAFNPQSTVIATGSMDTTAKLWDVQTG-AEISTLSGHSAEIISCAFNSTGTQLLTGSFDHTVSVWDTRSGR---RVHTLIGHRG-------EISNAQFNFD--CSFIVTGSMDKTCKIWDAGTGKCIGTLRGHDDEVLDVAFDYTGQLIATASADGTGRVYNALTHHCLSKLEGHEGEISKITFNAQ------------------------------------------GTRLLTASSDKTARLWDP---NTGKCLQVLEGHTDEIFSCAFNYDGDIIITGSKDNTCRLW 414
[Close]
BLAST of notchless-1 vs. Ensembl Nematostella
Match: EDO37614 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFB4])

HSP 1 Score: 98.9821 bits (245), Expect = 5.591e-22
Identity = 87/348 (25.00%), Postives = 153/348 (43.97%), Query Frame = 3 
Query:  474 HKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACME-NSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNL-IYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWK-------PEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTM 1490
            H K V    FS + R LA+ S D T R WD+   T  A    + H  +V   S SP G ++A++S    ++ W+L  G+      A+++ ++ A ++ +             + L +   D+T  IWD +L +P   +     SV    +    L I +G  + ++ +W A+ G     ++ H           D  +    F+P + S    +++      Q    L+ +              +DN + LW         EE + A    +  HQ+ V D  FSP+G  +ASAS D++V +W+ + G  L ++ GH + V   S+S D + L + S D +++LW +
Sbjct:   11 HTKDVTGCCFSANSRVLASCSGDKTARLWDVEKGTELAQSPLDGHNYHVNSCSVSPFGTLMATASTDSTLMLWNLETGE----CLAVLEGHTGAVRVCR--------FSPNSQFLISGSADETFIIWDVLLKKPVRCVDKLESSVTACAFTPDGLHIITGTSEGKLAIWEAQKGKFITQVEGH-----------DMGVGACDFSPTFGSAVPGLSDTSGSAPQ---FLLASGG------------NDNLVRLWTIYTASAFSEECHIASRCVLDQHQSQVWDCCFSPNGKILASASGDKTVILWNPLNGVALHTITGHSRYVTCCSFSPDGKWLATASGDRTLRLWQL 320

HSP 2 Score: 73.559 bits (179), Expect = 7.698e-14
Identity = 56/200 (28.00%), Postives = 92/200 (46.00%), Query Frame = 3 
Query: 1188 MVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDIS---FKKK----ITAPNCKR-----ARISHLLEALPGHADSVFALDWSPD----------------GMVVASGGKDGLVRLW 1703
            +  CS D T  LW  E+  +     + GH   VN    SP G  +A+AS D ++ +W+  TG+ L  L+GH   V +  +S +S+ L+S S D +  +W +  ++  +  +D+L+  ++   F       IT  +  +     A+    +  + GH   V A D+SP                   ++ASGG D LVRLW
Sbjct:   27 LASCSGDKTARLWDVEKGTELAQSPLDGHNYHVNSCSVSPFGTLMATASTDSTLMLWNLETGECLAVLEGHTGAVRVCRFSPNSQFLISGSADETFIIWDVL-LKKPVRCVDKLESSVTACAFTPDGLHIITGTSEGKLAIWEAQKGKFITQVEGHDMGVGACDFSPTFGSAVPGLSDTSGSAPQFLLASGGNDNLVRLW 225

HSP 3 Score: 59.3066 bits (142), Expect = 2.645e-9
Identity = 53/218 (24.31%), Postives = 93/218 (42.66%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCR-----LLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCM-------KWG-----GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD 1052
             C + + GH  AV V +FSP+ ++L +GSAD T   WD+  + P  C++   +S+V   +++P+G  + + +  G++  W    GK+  +          A     +       L  T       LLA+  +D  + +W TI      S   HI S RC+        W         ++ S   D  + +W   +GV   ++  HS ++   S + D
Sbjct:   89 ECLAVLEGHTGAVRVCRFSPNSQFLISGSADETFIIWDVLLKKPVRCVDK-LESSVTACAFTPDGLHIITGTSEGKLAIWEAQKGKFITQVEGH-DMGVGACDFSPTFGSAVPGLSDTSGSAPQFLLASGGNDNLVRLW-TIYTASAFSEECHIAS-RCVLDQHQSQVWDCCFSPNGKILASASGDKTVILWNPLNGVALHTITGHSRYVTCCSFSPD 302
[Close]
BLAST of notchless-1 vs. Ensembl Nematostella
Match: EDO33297 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSL6])

HSP 1 Score: 95.9005 bits (237), Expect = 1.736e-21
Identity = 55/161 (34.16%), Postives = 85/161 (52.80%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            +TGH   V+ VKFS +G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DSR LVS S D ++K+W  A  + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   61 LTGHNKAVSSVKFSHNGEWLASSSADKVIKIWGAYDGKFEKTITGHKLGISDVAWSTDSRHLVSASDDKTLKIWDFATGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVTAVHFNRDGALIVSSSYDGLCRIW 218

HSP 2 Score: 82.0333 bits (201), Expect = 6.081e-17
Identity = 79/297 (26.60%), Postives = 118/297 (39.73%), Query Frame = 3 
Query:  867 QPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            Q K +L+GH K+V  +K+      + S   D  I++W A DG   +++  H   I+ ++ +TD                                          +  +V  SDD TL +W  + +       + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V  + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    VFA      G  + SG +D  V +W
Sbjct:   56 QLKYTLTGHNKAVSSVKFSHNGEWLASSSADKVIKIWGAYDGKFEKTITGHKLGISDVAWSTD------------------------------------------SRHLVSASDDKTLKIW--DFATGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVTAVHFNRDGALIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHKVYIW 306

HSP 3 Score: 66.6254 bits (161), Expect = 6.613e-12
Identity = 71/308 (23.05%), Postives = 130/308 (42.21%), Query Frame = 3 
Query:  813 LAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRI-GAFNPMYE----STKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWS---CDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGG--KDGLVRLW 1703
            LA++  DK I IW     + + +++GH   +  + W   S  + S  +D  +++W    G   +++K HS+++   + N    + + G+F+         T   +  L A      A+       R+GA  +V  S D    +W    S + +   +      V+ VKFSP+G +I +A+ D ++K+WD   GK L +  GH  E   +  +      + +VS S+D  + +W +           Q KE                    +++ L GH+D V      P   ++ASG    D  +++W
Sbjct:   80 LASSSADKVIKIWGAYDGKFEKTITGHKLGISDVAWSTDSRHLVSASDDKTLKIWDFATGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVTAVHF----NRDGA-LIVSSSYDGLCRIWDTA-SGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHKVYIWNL-----------QSKE--------------------VVQKLDGHSDVVLCCACHPTENIIASGALENDKTIKIW 350

HSP 4 Score: 63.1586 bits (152), Expect = 1.057e-10
Identity = 56/224 (25.00%), Postives = 92/224 (41.07%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCM----KWGGSNLIYSGGEDCEIRVWRAE------------DGVLCRSMKPHSHWINSISLNTDYAIRI 1067
            +C  ++ GH   V    F+P    + +GS D +VR WD+ T     C++   +H   V  + ++ +G ++ SSS  G    W   +G   Q    LI  ++     VK             + + AA  D T+ +WD    +   + +GH     C+       G   I SG ED ++ +W  +            D VLC +  P  + I S +L  D  I+I
Sbjct:  140 KCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTG---KCLKTLPAHSDPVTAVHFNRDGALIVSSSYDGLCRIWDTASG---QCLKTLIDDDNPPVSFVK--------FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHKVYIWNLQSKEVVQKLDGHSDVVLCCACHPTENIIASGALENDKTIKI 349

HSP 5 Score: 61.2326 bits (147), Expect = 4.661e-10
Identity = 72/324 (22.22%), Postives = 127/324 (39.20%), Query Frame = 3 
Query:  438 RPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            +P  +   ++ GH KAV   +FS +G +LA+ SAD  ++ W       E  +   HK  +  ++WS + + L S+S    +  W    GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +     LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+D+ + +W  +  +K +  ++ GH +VV      P    IAS +   D+++KIW
Sbjct:   52 KPNYQLKYTLTGHNKAVSSVKFSHNGEWLASSSADKVIKIWGAYDGKFEKTI-TGHKLGISDVAWSTDSRHLVSASDDKTLKIWDFATGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVTAVHFNRDGALIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCVFANFSVTGGKWIVSGSEDHKVYIWNLQ--SKEVVQKLDGHSDVVLCCACHPTENIIASGALENDKTIKIW 350
[Close]
BLAST of notchless-1 vs. Ensembl Nematostella
Match: EDO49767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFR6])

HSP 1 Score: 93.5893 bits (231), Expect = 4.058e-21
Identity = 84/350 (24.00%), Postives = 143/350 (40.86%), Query Frame = 3 
Query:  441 PITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVR--CMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            P+ R   ++ G  + +L    +PD + + TG AD ++R WD  T   E      H   V  L+ SP+   L S +    +  W +        T +L+      K  V  +   P     + R+++++YD KT+ IWD   C    SL GH   VR   +   G  L+ S  +D  +R+W  E      +++ HS  +  +  + D    I                                            S+D  + +W  E + +     + GH + +  V  +PDG  + S+  D  VK+W   +G+ L SL GH + V +++ S D   +VS S+D++ K+W
Sbjct:    4 PLLR---TIDGRSRTILGIAVTPDTKKIITGGADGSIRVWDYETG-KELNKLLDHTKLVYTLALSPHADFLVSGAFDHTVKIWDM-------DTLSLVHTLKGHKNWVSGVLVTP----DSKRIISSSYD-KTVKIWDVETCAFVNSLDGHDGHVRGIAITSDGRRLV-SASQDRTLRIWNLETFAHVSTLRGHSETVYCVCCSPDDKFAISG------------------------------------------SEDTMVKIWDLESAKE--VRSLVGHTSDIFAVAVTPDGSKVISSGDDTQVKVWSLESGEELASLHGHSESVRIVTVSPDGLTIVSGSEDATFKIW 292

HSP 2 Score: 92.0485 bits (227), Expect = 1.132e-20
Identity = 76/300 (25.33%), Postives = 128/300 (42.67%), Query Frame = 3 
Query:  831 DKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTK--------TNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            D +I +WD    +    L  H K V  +     ++ + SG  D  +++W  +   L  ++K H +W++ + +  D + RI   +  Y+ T           +N+LD  +   R + I +  RR     +V  S D TL +W  E    A    + GH   V  V  SPD  F  S S D  VKIWD  + K + SL GH  ++  ++ + D   ++S   D+ +K+W++                                    L +L GH++SV  +  SPDG+ + SG +D   ++W
Sbjct:   34 DGSIRVWDYETGKELNKLLDHTKLVYTLALSPHADFLVSGAFDHTVKIWDMDTLSLVHTLKGHKNWVSGVLVTPD-SKRI--ISSSYDKTVKIWDVETCAFVNSLDGHDGHVRGIAITSDGRR-----LVSASQDRTLRIWNLE--TFAHVSTLRGHSETVYCVCCSPDDKFAISGSEDTMVKIWDLESAKEVRSLVGHTSDIFAVAVTPDGSKVISSGDDTQVKVWSLES-------------------------------GEELASLHGHSESVRIVTVSPDGLTIVSGSEDATFKIW 292

HSP 3 Score: 88.9669 bits (219), Expect = 1.544e-19
Identity = 76/259 (29.34%), Postives = 122/259 (47.10%), Query Frame = 3 
Query:  987 GVLCRSMKPHSHWINSISLNTDYA--IRIGAFNPM----YESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW---TMAQIRGL-----------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            G L R++   S  I  I++  D    I  GA   +    YE+ K      +  +L     L+Y  A    A+ +V  + D+T+ +W  +    ++   + GH+N V+ V  +PD   I S+S+D++VKIWD  T  F+ SL GH   V  I+ + D R LVS SQD ++++W   T A +  L             P D+     S    +   + + A+    + +L GH   +FA+  +PDG  V S G D  V++W
Sbjct:    3 GPLLRTIDGRSRTILGIAVTPDTKKIITGGADGSIRVWDYETGK------ELNKLLDHTKLVYTLALSPHADFLVSGAFDHTVKIW--DMDTLSLVHTLKGHKNWVSGVLVTPDSKRIISSSYDKTVKIWDVETCAFVNSLDGHDGHVRGIAITSDGRRLVSASQDRTLRIWNLETFAHVSTLRGHSETVYCVCCSPDDKFAISGSEDTMVKIWDLESAK---EVRSLVGHTSDIFAVAVTPDGSKVISSGDDTQVKVW 250
[Close]
BLAST of notchless-1 vs. Ensembl Medaka
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:101155788])

HSP 1 Score: 405.986 bits (1042), Expect = 7.161e-136
Identity = 224/545 (41.10%), Postives = 310/545 (56.88%), Query Frame = 3 
Query:   75 NDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            ++ V  + +QF D DGV  G  F +P     + L    N +L KE P    ++FF     E               + +S+ +C  +KA              G+ET  VL +VY PQ+VFRVR ++RCTS++ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W  + G      + + K  +   K +  L W+PLHL+  CR LA+   D ++ IWDT L +    L+GH +SV C+KWGG  L+Y+   D  ++VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P   +          EE+++RAL  YN  R +  ER+V  SDD TL LW P    K +  RMTGH  +VN+V FSPD   +ASASFD+SVKIWDG TGK+L+SL+GH+  V  ++WS DSRLLVS S DS++K+W +                               +   L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    3 SEDVERLLIQFQDEDGVVLGSPFDVPLDITPEKLQLVCNSLLMKEEPVP--FAFFARGEAE---------------VVSSLGSC--VKAI-------------GVETEQVLPVVYQPQAVFRVRAVSRCTSTLQGHTEAVISTAFSPTGKYLASGSGDTTVRFWDLSTETPHHTARG-HTHWVLSIAWSPDGKKLASGCKNSQICLWDPVTG------AQIGKTLTGHTKWITWLCWEPLHLNPECRYLASTSKDGSVRIWDTALGRCDKILTGHTQSVTCVKWGGDGLLYTSSHDRTVKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPATATINPQDVTGALEEVKERALQRYNKVRGSNPERLVSGSDDFTLFLWNPAADKKPL-ARMTGHSALVNEVLFSPDTRLLASASFDKSVKIWDGRTGKYLMSLRGHVGSVYQVAWSADSRLLVSGSSDSTLKVWDV-------------------------------KTGKLSIDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 476

HSP 2 Score: 51.6026 bits (122), Expect = 1.695e-6
Identity = 40/153 (26.14%), Postives = 63/153 (41.18%), Query Frame = 3 
Query:  522 LATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            L +GS D T+  W+             H + V  + +SP+ ++LAS+S    +  W    GK+       +         V  +AW         RLL +   D T+ +WD    +  I L GH   V  + W      + SGG+D  +R+WR
Sbjct:  337 LVSGSDDFTLFLWNPAADKKPLARMTGHSALVNEVLFSPDTRLLASASFDKSVKIWDGRTGKYLMSLRGHVGS-------VYQVAWS-----ADSRLLVSGSSDSTLKVWDVKTGKLSIDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 477
[Close]
BLAST of notchless-1 vs. Ensembl Medaka
Match: nle1 (notchless homolog 1 [Source:NCBI gene;Acc:101155788])

HSP 1 Score: 384.415 bits (986), Expect = 1.093e-127
Identity = 218/542 (40.22%), Postives = 299/542 (55.17%), Query Frame = 3 
Query:   84 VRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIET--VLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            V  + +QF D DGV  G  F +P     + L    N +L KE P    ++FF     E               + +S+ +C K                 G+ET  VL +VY PQ+VFRVR ++RCTS++ GH +AV+   FSP G+YLA+GS D TVRFWDL+T+TP       H   VL ++WSP+GK LAS     +I  W  + G      + + K  +   K +  L W+PLHL+  CR LA+   D ++ IWDT L +    L+GH +SV C+KWGG  L+Y+   D  ++VWRA+DGV CR+++ H+HW+N+++L+TDY +R GAF P    T           LQ    +     + +  ER+V  SDD TL LW P    K +  RMTGH  +VN+V FSPD   +ASASFD+SVKIWDG TGK+L+SL+GH+  V  ++WS DSRLLVS S DS++K+W +                               +   L   LPGHAD V+A+DWSPDG  VASGGKD  +R+W
Sbjct:    5 VERLLIQFQDEDGVVLGSPFDVPLDITPEKLQLVCNSLLMKEEPVP--FAFFARGEAE---------------VVSSLGSCVK---------------AIGVETEQVLPVVYQPQAVFRVRAVSRCTSTLQGHTEAVISTAFSPTGKYLASGSGDTTVRFWDLSTETPHHTARG-HTHWVLSIAWSPDGKKLASGCKNSQICLWDPVTG------AQIGKTLTGHTKWITWLCWEPLHLNPECRYLASTSKDGSVRIWDTALGRCDKILTGHTQSVTCVKWGGDGLLYTSSHDRTVKVWRAKDGVQCRTLQGHAHWVNTLALSTDYVLRTGAFEPSPART----------PLQHSGFV----PQGSNPERLVSGSDDFTLFLWNPAADKKPL-ARMTGHSALVNEVLFSPDTRLLASASFDKSVKIWDGRTGKYLMSLRGHVGSVYQVAWSADSRLLVSGSSDSTLKVWDV-------------------------------KTGKLSIDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIW 461

HSP 2 Score: 52.7582 bits (125), Expect = 8.341e-7
Identity = 43/163 (26.38%), Postives = 66/163 (40.49%), Query Frame = 3 
Query:  501 FSPDG---RYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWR 977
            F P G     L +GS D T+  W+             H + V  + +SP+ ++LAS+S    +  W    GK+       +         V  +AW         RLL +   D T+ +WD    +  I L GH   V  + W      + SGG+D  +R+WR
Sbjct:  312 FVPQGSNPERLVSGSDDFTLFLWNPAADKKPLARMTGHSALVNEVLFSPDTRLLASASFDKSVKIWDGRTGKYLMSLRGHVGS-------VYQVAWS-----ADSRLLVSGSSDSTLKVWDVKTGKLSIDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWR 462
[Close]
BLAST of notchless-1 vs. Ensembl Medaka
Match: wdr5 (WD repeat domain 5 [Source:NCBI gene;Acc:101166483])

HSP 1 Score: 93.9745 bits (232), Expect = 1.313e-20
Identity = 54/161 (33.54%), Postives = 84/161 (52.17%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + GH   V+ VKFSP G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DS LLVS S D ++K+W +   + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   41 LAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVNSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 198

HSP 2 Score: 85.5001 bits (210), Expect = 9.118e-18
Identity = 81/296 (27.36%), Postives = 124/296 (41.89%), Query Frame = 3 
Query:  873 KISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL--------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            K +L+GH K+V  +K+  S   + S   D  I++W A DG   +++  H   I+ ++ ++D                   +NL                       +V  SDD TL +W   + N   C + + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L              L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   38 KFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSD-------------------SNL-----------------------LVSASDDKTLKIW---DVNSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 286

HSP 3 Score: 67.781 bits (164), Expect = 7.187e-12
Identity = 75/324 (23.15%), Postives = 130/324 (40.12%), Query Frame = 3 
Query:  438 RPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNP--MYESTKTNINNLDAEELQKRALLIYNTARRN------------GAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            +P      ++ GH KAV   +FSP G +LA+ SAD  ++ W       E  + + HK  +  ++WS +  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++            + +  +    F+P   Y    T  N L   +  K   L   T  +N            G + +V  S+DN + +W  +   K I  ++ GH +VV      P    IASA+   D+++K+W
Sbjct:   32 KPNYSLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVNSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLID----------DDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--TKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW 330
[Close]
BLAST of notchless-1 vs. Ensembl Medaka
Match: wdr5 (WD repeat domain 5 [Source:NCBI gene;Acc:101166483])

HSP 1 Score: 93.9745 bits (232), Expect = 1.395e-20
Identity = 54/161 (33.54%), Postives = 84/161 (52.17%), Query Frame = 3 
Query: 1263 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL--------------LVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            + GH   V+ VKFSP G ++AS+S D+ +KIW    GKF  ++ GH   ++ ++WS DS LLVS S D ++K+W +   + L                P   L    SF + +   + K  +    L+ LP H+D V A+ ++ DG ++ S   DGL R+W
Sbjct:   40 LAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVNSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGK---CLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIW 197

HSP 2 Score: 85.5001 bits (210), Expect = 1.110e-17
Identity = 79/298 (26.51%), Postives = 122/298 (40.94%), Query Frame = 3 
Query:  873 KISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR-MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHL----------------LEALPGHADS---VFALDWSPDGMVVASGGKDGLVRLW 1703
            K +L+GH K+V  +K+  S   + S   D  I++W A DG   +++  H   I+ ++ ++D                                          +  +V  SDD TL +W   + N   C + + GH N V    F+P    I S SFD SV+IWD  TGK L +L  H   V+ + ++ D  L+VS S D   ++W  A  + L   ID     +SF K   +PN K    + L                L+   GH +    +FA      G  + SG +D +V +W
Sbjct:   37 KFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSD------------------------------------------SNLLVSASDDKTLKIW---DVNSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVK--FSPNGKYILAATLDNSSLTFVFFYVNSQCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIW 287

HSP 3 Score: 68.1662 bits (165), Expect = 6.323e-12
Identity = 74/324 (22.84%), Postives = 129/324 (39.81%), Query Frame = 3 
Query:  438 RPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSM-------------KPHSHWINSISL-NTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASF--DRSVKIW 1358
            +P      ++ GH KAV   +FSP G +LA+ SAD  ++ W       E  + + HK  +  ++WS +  +L S+S    +  W + +GK        +K        V    + P        L+ +   D+++ IWD    +   +L  H   V  + +    +LI S   D   R+W    G   +++              P+  +I + +L N+         N     T T   N            I+      G + +V  S+DN + +W  +   K I  ++ GH +VV      P    IASA+   D+++K+W
Sbjct:   31 KPNYSLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTI-SGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVNSGK-------CLKTLKGHSNYVFCCNFNP-----QSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNSSLTFVFFYVNSQCLKTYTGHKN--------EKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQ--TKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLW 331
[Close]
BLAST of notchless-1 vs. Ensembl Medaka
Match: POC1A (POC1 centriolar protein A [Source:NCBI gene;Acc:101161307])

HSP 1 Score: 93.5893 bits (231), Expect = 8.504e-20
Identity = 89/370 (24.05%), Postives = 142/370 (38.38%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG--GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVV-CSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            H+  V  + +S N K +A+ SV   ++ W++       K      +    K  V S+ + P     +  ++A+A  DKT+ +W   +         H  +VRC+ +   G  L+ +   D  I+VW         S   H +W+           R   F+P                                 +R++V CSDD T+ LW  +++++        H    N V F P G  IA+AS D SVK+WD  T K L   Q H   VN +S+      L++ S DS++K+  + + +                               LL  L GH  SV  + +S  G   +SGG D  V +W
Sbjct:   17 HRGAVTSVDFSCNMKQIATGSVDSCVMIWNM-------KPQMRAYRFEGHKDAVTSVQFSP-----SGHMVASASRDKTVRLWVPSIKADSTEFRAHTAAVRCVNFSDDGQTLV-TASNDKTIKVWTVHRQKFLFSFNQHVNWV-----------RCAKFSP--------------------------------DDRLIVSCSDDKTIKLW--DKNSRECIQSFFEHAGYANHVDFHPSGTCIAAASTDSSVKLWDIRTNKMLQHYQVHSGAVNSLSFHPAGNFLITTSSDSTVKILDLLEGK-------------------------------LLYTLHGHQSSVTCVAFSRTGDYFSSGGADEQVMVW 297

HSP 2 Score: 89.3521 bits (220), Expect = 1.570e-18
Identity = 83/339 (24.48%), Postives = 137/339 (40.41%), Query Frame = 3 
Query:  471 GHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSN-LIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            GH+ AV    FS + + +ATGS D+ V  W++  Q      E  HK  V  + +SP+G ++AS+S    +  W              IK +S+  +   + A + ++     + L  A +DKTI +W     +   S + H+  VRC K+   + LI S  +D  I++W        +S   H+ + N +            F+P                                   +   S D+++ LW    +NK +      H   VN + F P G F+ + S D +VKI D + GK L +L GH   V  +++S       S   D  + +W
Sbjct:   16 GHRGAVTSVDFSCNMKQIATGSVDSCVMIWNMKPQMRAYRFEG-HKDAVTSVQFSPSGHMVASASRDKTVRLW-----------VPSIKADSTEFR-AHTAAVRCVNFSDDGQTLVTASNDKTIKVWTVHRQKFLFSFNQHVNWVRCAKFSPDDRLIVSCSDDKTIKLWDKNSRECIQSFFEHAGYANHVD-----------FHP-------------------------------SGTCIAAASTDSSVKLWDI-RTNKML-QHYQVHSGAVNSLSFHPAGNFLITTSSDSTVKILDLLEGKLLYTLHGHQSSVTCVAFSRTGDYFSSGGADEQVMVW 297

HSP 3 Score: 50.447 bits (119), Expect = 3.988e-6
Identity = 37/156 (23.72%), Postives = 65/156 (41.67%), Query Frame = 3 
Query: 1269 GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMA------QIRGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            GH+  V  V FS +   IA+ S D  V IW+          +GH   V  + +S    ++ S S+D +++LW  +      + R     +  +      +  +TA N K  ++        L +   H + V    +SPD  ++ S   D  ++LW
Sbjct:   16 GHRGAVTSVDFSCNMKQIATGSVDSCVMIWNMKPQMRAYRFEGHKDAVTSVQFSPSGHMVASASRDKTVRLWVPSIKADSTEFRAHTAAVRCVNFSDDGQTLVTASNDKTIKVWTVHRQKFLFSFNQHVNWVRCAKFSPDDRLIVSCSDDKTIKLW 171
[Close]
BLAST of notchless-1 vs. Planmine SMEST
Match: SMESG000000678.1 (SMESG000000678.1)

HSP 1 Score: 1129.78 bits (2921), Expect = 0.000e+0
Identity = 545/547 (99.63%), Postives = 546/547 (99.82%), Query Frame = 3 
Query:   63 MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQS+YSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW
Sbjct:    1 MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEGPEQSLYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNETKENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENGHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 547

HSP 2 Score: 57.3806 bits (137), Expect = 2.336e-8
Identity = 47/173 (27.17%), Postives = 77/173 (44.51%), Query Frame = 3 
Query:  465 MPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIY-SGGEDCEIRVWRA 980
            M GH+  V   +FSPDG ++A+ S D +V+ WD  T      ++  H   V L+SWS + ++L S S    I  W++   +        +K++ S KK + +   K   +     LL A                    L GH  SV  + W    ++  SGG+D  +R+W++
Sbjct:  401 MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQ-GHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARIS---HLLEA--------------------LPGHADSVFALDWSPDGMVVASGGKDGLVRLWKS 549
[Close]
BLAST of notchless-1 vs. Planmine SMEST
Match: SMESG000024482.1 (SMESG000024482.1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.712e-20
Identity = 82/368 (22.28%), Postives = 134/368 (36.41%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNL-IYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            HK  V  + ++PN   LAS  +   ++ W+        K      +    +  + S+ + P     T +L+A+A  DKT+ +W   +    +    H  +VR +     NL + +G +D  I+VW+        S+  H +W+  +  ++D                                           + +   SDD T+ LW  +  N+        H   V  + F P+G +IAS   D  V IWD    K L     H   VN +S+      L+S S DSS+K++ + + R                               LL  + GH   +    +S  G + ASGG D  V LW
Sbjct:   13 HKDKVTCVDFNPNSSQLASGGIDSCLMIWNF-------KPQTRAYRFVGHEDAILSVKFSP-----TGQLVASASRDKTVRLWVPSIKGESLVFRAHTATVRSVDISCDNLEMVTGSDDKTIKVWKMHRQKFSYSLNEHKNWVRCVRFSSD------------------------------------------GQLIASSSDDKTIKLW--DRHNQECIHTFQEHGGFVPSLAFHPNGSYIASGGTDSHVNIWDIRMNKLLQHYTSHTSPVNAVSFHPSGNFLISSSDDSSMKIYDLLEGR-------------------------------LLYTVQGHEGPILGTAFSHCGEMFASGGADHQVFLW 293

HSP 2 Score: 92.4337 bits (228), Expect = 1.414e-19
Identity = 95/383 (24.80%), Postives = 159/383 (41.51%), Query Frame = 3 
Query:  471 GHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLL--AAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIR--GLLV------PIDQLKEDISFKKKITAPNCKRAR 1586
            GHK  V    F+P+   LA+G  D+ +  W+   QT  A     H+  +L + +SP G+++AS+S    +  W       S K  +L+ +  +A   V+S       +D +C  L      DDKTI +W     +   SL+ H   VRC+++     LI S  +D  I++W   +     + + H  ++ S+           AF+P                              NG+  +     D+ +++W     NK +    T H + VN V F P G F+ S+S D S+KI+D + G+ L ++QGH   +   ++S    +  S   D  + LW   Q+   G+ V      P            K+T P  +R +
Sbjct:   12 GHKDKVTCVDFNPNSSQLASGGIDSCLMIWNFKPQT-RAYRFVGHEDAILSVKFSPTGQLVASASRDKTVRLWVP-----SIKGESLVFRAHTAT--VRS-------VDISCDNLEMVTGSDDKTIKVWKMHRQKFSYSLNEHKNWVRCVRFSSDGQLIASSSDDKTIKLWDRHNQECIHTFQEHGGFVPSL-----------AFHP------------------------------NGS-YIASGGTDSHVNIWDI-RMNK-LLQHYTSHTSPVNAVSFHPSGNFLISSSDDSSMKIYDLLEGRLLYTVQGHEGPILGTAFSHCGEMFASGGADHQVFLWQAGQLSEPGIAVHSGSSQPQHHATTGTKSTSKLTQPVVRRTQ 335

HSP 3 Score: 58.9214 bits (141), Expect = 7.212e-9
Identity = 52/208 (25.00%), Postives = 96/208 (46.15%), Query Frame = 3 
Query:  426 VFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLN 1046
            ++  +P TR    + GH+ A+L  +FSP G+ +A+ S D TVR W + +   E+ +  +H + V  +  S +   + + S    I  W +   K+S          +  K  V+ + +     DG  +L+A++ DDKTI +WD    +   +   H   V  + +    + I SGG D  + +W      L +    H+  +N++S +
Sbjct:   40 IWNFKPQTRAYRFV-GHEDAILSVKFSPTGQLVASASRDKTVRLW-VPSIKGESLVFRAHTATVRSVDISCDNLEMVTGSDDKTIKVWKMHRQKFSYSL-------NEHKNWVRCVRFSS---DG--QLIASSSDDKTIKLWDRHNQECIHTFQEHGGFVPSLAFHPNGSYIASGGTDSHVNIWDIRMNKLLQHYTSHTSPVNAVSFH 233

HSP 4 Score: 50.447 bits (119), Expect = 3.322e-6
Identity = 40/159 (25.16%), Postives = 69/159 (43.40%), Query Frame = 3 
Query: 1269 GHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWT-------------MAQIRGLLVPIDQLKEDI-SFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            GH++ V  V F+P+   +AS   D  + IW+           GH   +  + +S   +L+ S S+D +++LW               A +R + +  D L+    S  K I      R + S+ L     H + V  + +S DG ++AS   D  ++LW
Sbjct:   12 GHKDKVTCVDFNPNSSQLASGGIDSCLMIWNFKPQTRAYRFVGHEDAILSVKFSPTGQLVASASRDKTVRLWVPSIKGESLVFRAHTATVRSVDISCDNLEMVTGSDDKTIKVWKMHRQKFSYSLNE---HKNWVRCVRFSSDGQLIASSSDDKTIKLW 167
[Close]
BLAST of notchless-1 vs. Planmine SMEST
Match: SMESG000008519.1 (SMESG000008519.1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.031e-19
Identity = 70/297 (23.57%), Postives = 136/297 (45.79%), Query Frame = 3 
Query:  876 ISLSGHIKSVRCMKWG--GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLN-TDYAIRIGAFNPM-----YESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWD-GMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQI-------RGLLVPIDQLKEDISFKKKITAPNCKRARI-----SHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
             +L GH   V  + +    S+ I +G  D   ++W A DG L  + K H+  +  +  N +   +  G  + +      ES K  ++NL     +  +L      + +  + ++  S D+T+ LW      +    ++ GH   ++   F+ DG ++A+AS D+S+KIWD  M  + L +L GH  EV  +++    + + S   D++++LW +          +G    + ++  +      ++A   K AR        +++ L GH D +F+  ++ DG  + +G KD   R+W
Sbjct:    6 FTLEGHKNVVYSIAYNLPFSDKIVTGSFDKTAKMWSANDGSLYHTFKGHTAEVVCVQFNPSSTLVGTGGMDTLAMLWDVESGKC-VSNLQGHTAEVISLQF---GQAHNGKHLITGSFDHTVILWDVHNGRR--VSQLIGHDAEISAAVFNFDGTYVATASMDKSIKIWDLRMGARDLFTLAGHTDEVLDVTFDNVGKQVASAGADNTVRLWDVKDFSKPSFIFKGHEAEVSKVTFNPQGTVLLSASADKSARTWNTTTGKVIQVLEGHTDEIFSCAFNYDGSKIITGSKDNTCRIW 296

HSP 2 Score: 70.8626 bits (172), Expect = 4.265e-13
Identity = 56/239 (23.43%), Postives = 108/239 (45.19%), Query Frame = 3 
Query:  810 LLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWG----GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARR----------NGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLW 1484
            L+     D    +WD    +   +L GH   V  +++G    G +LI +G  D  + +W   +G     +  H   I++   N         F+  Y +T +   ++   +L+  A  ++  A            N  +++     DNT+ LW  ++ +K       GH+  V+ V F+P G  + SAS D+S + W+  TGK +  L+GH  E+   +++ D   +++ S+D++ ++W
Sbjct:   69 LVGTGGMDTLAMLWDVESGKCVSNLQGHTAEVISLQFGQAHNGKHLI-TGSFDHTVILWDVHNGRRVSQLIGHDAEISAAVFN---------FDGTYVATASMDKSIKIWDLRMGARDLFTLAGHTDEVLDVTFDNVGKQVASAGADNTVRLWDVKDFSKP-SFIFKGHEAEVSKVTFNPQGTVLLSASADKSARTWNTTTGKVIQVLEGHTDEIFSCAFNYDGSKIITGSKDNTCRIW 296

HSP 3 Score: 67.781 bits (164), Expect = 4.021e-12
Identity = 63/263 (23.95%), Postives = 109/263 (41.44%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMEN--SHKSNVLLLSW--SPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTIL-CQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAED-GVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWK 1229
            +  GH   V+  QF+P    + TG  D     WD+ +     C+ N   H + V+ L +  + NGK L + S    +I W + NG+   + S LI  ++     V        + DGT   +A A  DK+I IWD  +  +   +L+GH   V  + +      + S G D  +R+W  +D        K H   ++ ++ N    + + A       T         + L+     I++ A      +++  S DNT  +W+
Sbjct:   50 TFKGHTAEVVCVQFNPSSTLVGTGGMDTLAMLWDVESG---KCVSNLQGHTAEVISLQFGQAHNGKHLITGSFDHTVILWDVHNGR---RVSQLIGHDAEISAAV-------FNFDGT--YVATASMDKSIKIWDLRMGARDLFTLAGHTDEVLDVTFDNVGKQVASAGADNTVRLWDVKDFSKPSFIFKGHEAEVSKVTFNPQGTVLLSASADKSARTWNTTTGKVIQVLEGHTDEIFSCAFNYDGSKIITGSKDNTCRIWQ 297

HSP 4 Score: 59.3066 bits (142), Expect = 2.111e-9
Identity = 49/178 (27.53%), Postives = 80/178 (44.94%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVR--CMKWGGSNLIYSGGEDCEIRVWR 977
            R  S + GH   +  A F+ DG Y+AT S D +++ WDL     +      H   VL +++   GK +AS+     +  W +   K   K S + K + +    V  + + P    GT  L A+A  DK+   W+T   +    L GH   +      + GS +I +G +D   R+W+
Sbjct:  132 RRVSQLIGHDAEISAAVFNFDGTYVATASMDKSIKIWDLRMGARDLFTLAGHTDEVLDVTFDNVGKQVASAGADNTVRLWDV---KDFSKPSFIFKGHEAE---VSKVTFNP---QGTVLLSASA--DKSARTWNTTTGKVIQVLEGHTDEIFSCAFNYDGSKII-TGSKDNTCRIWQ 297

HSP 5 Score: 55.0694 bits (131), Expect = 5.846e-8
Identity = 47/205 (22.93%), Postives = 89/205 (43.41%), Query Frame = 3 
Query:  450 RCTSSMPGHKKAVLVAQF--SPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTI-LCQPKISLSGHIKSVRCMKWG-GSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTD 1052
            +C S++ GH   V+  QF  + +G++L TGS D+TV  WD++     + +   H + +    ++ +G  +A++S+   I  W L  G        L        +++       +  D   + +A+A  D T+ +WD     +P     GH   V  + +     ++ S   D   R W    G + + ++ H+  I S + N D
Sbjct:   88 KCVSNLQGHTAEVISLQFGQAHNGKHLITGSFDHTVILWDVHNGRRVSQLIG-HDAEISAAVFNFDGTYVATASMDKSIKIWDLRMG-----ARDLFTLAGHTDEVL------DVTFDNVGKQVASAGADNTVRLWDVKDFSKPSFIFKGHEAEVSKVTFNPQGTVLLSASADKSARTWNTTTGKVIQVLEGHTDEIFSCAFNYD 280

HSP 6 Score: 53.5286 bits (127), Expect = 2.082e-7
Identity = 28/94 (29.79%), Postives = 48/94 (51.06%), Query Frame = 3 
Query:  426 VFRVRPITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGK 707
            ++ +R   R   ++ GH   VL   F   G+ +A+  ADNTVR WD+   +  + +   H++ V  ++++P G VL S+S       W+   GK
Sbjct:  167 IWDLRMGARDLFTLAGHTDEVLDVTFDNVGKQVASAGADNTVRLWDVKDFSKPSFIFKGHEAEVSKVTFNPQGTVLLSASADKSARTWNTTTGK 260
[Close]
BLAST of notchless-1 vs. Planmine SMEST
Match: SMESG000057676.1 (SMESG000057676.1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.093e-16
Identity = 90/380 (23.68%), Postives = 148/380 (38.95%), Query Frame = 3 
Query:  441 PITRCTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLL------------------LSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDG---------LFIASASFDRSVKIWD---GMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTM 1490
            P  R T+ + GH   V +  ++P    LA+GS D+T R W+L   T +   ++++K  VL                   L W+ +G +LA+ S  G    W+ I+G        L+      K  + +L W      G   L A    DKT  +W+          S H      + W  +    S   D +I + +  +  + ++ K H + +N+I  +       G F                                     +  CSDD +L LW   E N        GH   +  +++S  G         L++ASASFD +V++WD   G+  K+L    GH + V  +S+S D R L + S D ++ +W +
Sbjct:  237 PTNRVTT-LKGHTSEVFICAWNPVKDLLASGSGDSTARIWNL---TSDNYFDHTNKELVLTHCVNRGGQNVLSNKDVTSLDWNTDGSLLATGSYDGFARIWN-IDG-------TLVSTLGQHKGPIFALKWNK---KGNYVLTAGV--DKTTIVWEASTNTMIQQFSFHKAPTLDVDWKDAKTFGSCSTDSKIHICKVGNTSVVKTFKGHENEVNAIKWDP-----TGRF-------------------------------------LASCSDDKSLKLWSMNEDN--CVQNFVGHTKEIYTIQWSKTGPNTDYPNSPLYLASASFDSTVRLWDIERGICSKYLT---GHTEPVYSVSFSPDGRFLATGSFDQNVMIWNV 552

HSP 2 Score: 79.337 bits (194), Expect = 3.094e-15
Identity = 94/388 (24.23%), Postives = 150/388 (38.66%), Query Frame = 3 
Query:  603 HKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALI---------KKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIW--DTILCQPKISLSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWS--------CDSRL-LVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLW 1703
            H S V + +W+P   +LAS S       W+L +  +   T+  +          +N  + K V SL W   + DG+  LLA    D    IW  D  L     +L  H   +  +KW    N + + G D    VW A    + +    H     + +L+ D+                                         A+    CS D+ + + K    N ++     GH+N VN +K+ P G F+AS S D+S+K+W       + +  GH +E+  I WS         +S L L S S DS+++LW +   RG+                     C +         L GH + V+++ +SPDG  +A+G  D  V +W
Sbjct:  247 HTSEVFICAWNPVKDLLASGSGDSTARIWNLTSDNYFDHTNKELVLTHCVNRGGQNVLSNKDVTSLDW---NTDGS--LLATGSYDGFARIWNIDGTLVS---TLGQHKGPIFALKWNKKGNYVLTAGVDKTTIVWEASTNTMIQQFSFH----KAPTLDVDWK---------------------------------------DAKTFGSCSTDSKIHICKV--GNTSVVKTFKGHENEVNAIKWDPTGRFLASCSDDKSLKLWSMNEDNCVQNFVGHTKEIYTIQWSKTGPNTDYPNSPLYLASASFDSTVRLWDIE--RGI---------------------CSK--------YLTGHTEPVYSVSFSPDGRFLATGSFDQNVMIW 550

HSP 3 Score: 50.447 bits (119), Expect = 3.703e-6
Identity = 30/89 (33.71%), Postives = 42/89 (47.19%), Query Frame = 3 
Query:  423 SVFRVRPITR--CTSSMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEII 683
            S  R+  I R  C+  + GH + V    FSPDGR+LATGS D  V  W  N          S    +  + W+  G  +A+S+  G I+
Sbjct:  503 STVRLWDIERGICSKYLTGHTEPVYSVSFSPDGRFLATGSFDQNVMIW--NVDRGSVIHTYSCSGGIFEVCWNSRGDKVAASASDGSIV 589
[Close]
BLAST of notchless-1 vs. Planmine SMEST
Match: SMESG000035997.1 (SMESG000035997.1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.133e-16
Identity = 67/279 (24.01%), Postives = 124/279 (44.44%), Query Frame = 3 
Query:  882 LSGHIKSVRCMKWGG-SNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMA--QIRGLLVPIDQLKEDISFKKKITAPNCKRARISHLLEALPGHADSVFALD-WSPDG-MVVASGGKDGLVRLW 1703
            L GH  S+ C+++    N+  SG ED  I++W  E G   R+++ H++ +  +           AF+P                                 + +  CS D  + LW   E        ++GH + V+ V F P G  + SAS D+++K+W+  TG  + + +GH   V ++  + D  L+ SCS D+S+++W+MA  + +      + + + I +  +       +A +    + +  + DS   LD +   G  ++ASG +D  +RLW
Sbjct:  105 LKGHRASITCVRFHPLYNVFVSGSEDATIKIWDYESGDYERTLRGHTNHVQDL-----------AFDP-------------------------------SGKLLASCSADMQIKLWDFVEF--TCVKTLSGHDHNVSGVSFMPSGDHLLSASRDKTIKLWEVSTGYCVQTFEGHSDWVRVVKPNFDGSLIASCSNDNSVRVWSMANKECKFDFREHEHVVQCIVWGGQKIHMETNQALLDD--KQMTSNGDSSNKLDGYKMSGCHLLASGSRDRTIRLW 337

HSP 2 Score: 68.5514 bits (166), Expect = 4.686e-12
Identity = 82/350 (23.43%), Postives = 134/350 (38.29%), Query Frame = 3 
Query:  465 MPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMK--WGGSNLIYSGGEDCEIRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNINNLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGRMTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEVNLISWSCDSRLLVSCSQDSSIKLWTMAQIRGL 1508
            + GH+ ++   +F P      +GS D T++ WD  +   E  +   H ++V  L++ P+GK+LAS S   +I  W  +           +K  S     V  +++ P     +   L +A  DKTI +W+        +  GH   VR +K  + GS LI S   D  +RVW   +       + H H +  I               ++   K ++          +ALL       NG                  + SNK    +M+G                +AS S DR++++WD  TG  L +L           WSCD    + CS+ S  +L  +   + L
Sbjct:  105 LKGHRASITCVRFHPLYNVFVSGSEDATIKIWDYESGDYERTLRG-HTNHVQDLAFDPSGKLLASCSADMQIKLWDFVE-------FTCVKTLSGHDHNVSGVSFMP-----SGDHLLSASRDKTIKLWEVSTGYCVQTFEGHSDWVRVVKPNFDGS-LIASCSNDNSVRVWSMANKECKFDFREHEHVVQCI---------------VWGGQKIHMET-------NQALLDDKQMTSNG------------------DSSNKLDGYKMSGCH-------------LLASGSRDRTIRLWDTNTGVCLFNL----------VWSCD---FIDCSKLSIFRLAIIIGFKSL 374

HSP 3 Score: 57.3806 bits (137), Expect = 1.559e-8
Identity = 43/158 (27.22%), Postives = 72/158 (45.57%), Query Frame = 3 
Query:  462 SMPGHKKAVLVAQFSPDGRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASSSVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRLLAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNL 935
            ++ GH   V    F P G+ LA+ SAD  ++ WD    T    + + H  NV  +S+ P+G  L S+S    I  W +  G   Q      + +S   ++VK       + DG+  L+A+  +D ++ +W     + K     H   V+C+ WGG  +
Sbjct:  146 TLRGHTNHVQDLAFDPSGKLLASCSADMQIKLWDFVEFTCVKTL-SGHDHNVSGVSFMPSGDHLLSASRDKTIKLWEVSTGYCVQT----FEGHSDWVRVVKP------NFDGS--LIASCSNDNSVRVWSMANKECKFDFREHEHVVQCIVWGGQKI 290
The following BLAST results are available for this feature:
BLAST of notchless-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NLE11.273e-14041.58notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:1... [more]
NLE18.724e-11437.18notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:1... [more]
NLE12.073e-8042.12notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:1... [more]
NLE12.368e-5847.53notchless homolog 1 [Source:HGNC Symbol;Acc:HGNC:1... [more]
POC1B6.883e-2325.00POC1 centriolar protein B [Source:HGNC Symbol;Acc:... [more]
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BLAST of notchless-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
W07E6.23.031e-13245.92pep chromosome:WBcel235:II:480722:484386:-1 gene:W... [more]
W07E6.21.003e-3744.10pep chromosome:WBcel235:II:480722:483104:-1 gene:W... [more]
wdr-5.15.031e-1732.34WD repeat-containing protein wdr-5.1 [Source:UniP... [more]
wdr-5.15.031e-1732.34WD repeat-containing protein wdr-5.1 [Source:UniP... [more]
rack-11.633e-1631.43Guanine nucleotide-binding protein subunit beta-2-... [more]
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BLAST of notchless-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nle7.776e-13740.96gene:FBgn0021874 transcript:FBtr0078075[more]
wds2.935e-2134.16gene:FBgn0040066 transcript:FBtr0308566[more]
wds2.935e-2134.16gene:FBgn0040066 transcript:FBtr0070438[more]
Poc11.047e-1924.44gene:FBgn0036354 transcript:FBtr0075863[more]
Poc11.299e-1924.44gene:FBgn0036354 transcript:FBtr0321318[more]
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BLAST of notchless-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nle15.760e-13641.51notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:... [more]
nle15.760e-13641.51notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:... [more]
nle15.760e-13641.51notchless homolog 1 (Drosophila) [Source:ZFIN;Acc:... [more]
wdr52.175e-2132.56WD repeat domain 5 [Source:ZFIN;Acc:ZDB-GENE-04042... [more]
daw12.733e-2124.57dynein assembly factor with WDR repeat domains 1 [... [more]
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BLAST of notchless-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nle12.950e-14041.54notchless homolog 1 [Source:NCBI gene;Acc:779995][more]
nle14.043e-13941.44notchless homolog 1 [Source:NCBI gene;Acc:779995][more]
nle14.518e-13941.36notchless homolog 1 [Source:NCBI gene;Acc:779995][more]
nle16.107e-13941.44notchless homolog 1 [Source:NCBI gene;Acc:779995][more]
daw19.296e-2323.93dynein assembly factor with WDR repeat domains 1 [... [more]
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BLAST of notchless-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nle13.320e-13740.74notchless homolog 1 [Source:MGI Symbol;Acc:MGI:242... [more]
Poc1b1.348e-2225.00POC1 centriolar protein B [Source:MGI Symbol;Acc:M... [more]
Poc1b1.503e-2225.00POC1 centriolar protein B [Source:MGI Symbol;Acc:M... [more]
Poc1b1.629e-2225.00POC1 centriolar protein B [Source:MGI Symbol;Acc:M... [more]
Wdr53.787e-2233.14WD repeat domain 5 [Source:MGI Symbol;Acc:MGI:2155... [more]
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BLAST of notchless-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9NVX2|NLE1_HUMAN6.111e-14041.58Notchless protein homolog 1 OS=Homo sapiens OX=960... [more]
sp|Q58D20|NLE1_BOVIN6.314e-14042.22Notchless protein homolog 1 OS=Bos taurus OX=9913 ... [more]
sp|Q5RFF8|NLE1_PONAB5.677e-13941.85Notchless protein homolog 1 OS=Pongo abelii OX=960... [more]
sp|Q8VEJ4|NLE1_MOUSE2.322e-13640.74Notchless protein homolog 1 OS=Mus musculus OX=100... [more]
sp|Q7ZXK9|NLE1_XENLA6.482e-13640.99Notchless protein homolog 1 OS=Xenopus laevis OX=8... [more]
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BLAST of notchless-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
I1ZIF14.219e-999.63Notchless-1 OS=Schmidtea mediterranea OX=79327 PE=... [more]
A0A0R3T1V15.464e-16546.21WD_REPEATS_REGION domain-containing protein OS=Rod... [more]
A0A158QCT02.410e-16445.97WD_REPEATS_REGION domain-containing protein OS=Hym... [more]
A0A0X3PTC62.691e-16443.53Notchless protein homolog 1 OS=Schistocephalus sol... [more]
A0A4E0RD444.398e-16444.46Notchless protein 1 OS=Fasciola hepatica OX=6192 G... [more]
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BLAST of notchless-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nle11.883e-13741.54notchless homolog 1 [Source:NCBI gene;Acc:10304664... [more]
ENSAMXT00000007963.21.257e-2233.72WD repeat-containing protein 5-like [Source:NCBI g... [more]
ENSAMXT00000007962.21.669e-2233.72WD repeat-containing protein 5-like [Source:NCBI g... [more]
ENSAMXT00000052579.11.669e-2233.72WD repeat-containing protein 5-like [Source:NCBI g... [more]
wdr51.679e-2132.56WD repeat domain 5 [Source:NCBI gene;Acc:103033424... [more]
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BLAST of notchless-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002506.11.943e-1832.49pep scaffold:Pmarinus_7.0:GL479076:26529:32136:-1 ... [more]
snrnp405.451e-1823.35small nuclear ribonucleoprotein 40 (U5) [Source:ZF... [more]
POC1A6.633e-1823.24POC1 centriolar protein A [Source:HGNC Symbol;Acc:... [more]
taf5l1.458e-1730.77TAF5-like RNA polymerase II, p300/CBP-associated f... [more]
ciao11.680e-1724.87cytosolic iron-sulfur assembly component 1 [Source... [more]
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BLAST of notchless-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
RSA49.181e-10837.91WD-repeat protein involved in ribosome biogenesis;... [more]
PRP41.716e-1929.71Splicing factor; component of the U4/U6-U5 snRNP c... [more]
ASC12.182e-1629.23G-protein beta subunit and guanine dissociation in... [more]
CDC46.440e-1526.37F-box protein required for both the G1/S and G2/M ... [more]
TUP12.558e-1330.38General repressor of transcription; forms complex ... [more]
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BLAST of notchless-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO315551.730e-13942.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO348654.651e-2524.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO376145.591e-2225.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO332971.736e-2134.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO497674.058e-2124.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of notchless-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nle17.161e-13641.10notchless homolog 1 [Source:NCBI gene;Acc:10115578... [more]
nle11.093e-12740.22notchless homolog 1 [Source:NCBI gene;Acc:10115578... [more]
wdr51.313e-2033.54WD repeat domain 5 [Source:NCBI gene;Acc:101166483... [more]
wdr51.395e-2033.54WD repeat domain 5 [Source:NCBI gene;Acc:101166483... [more]
POC1A8.504e-2024.05POC1 centriolar protein A [Source:NCBI gene;Acc:10... [more]
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BLAST of notchless-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000000678.12.336e-899.63SMESG000000678.1[more]
SMESG000024482.13.712e-2022.28SMESG000024482.1[more]
SMESG000008519.11.031e-1923.57SMESG000008519.1[more]
SMESG000057676.12.093e-1623.68SMESG000057676.1[more]
SMESG000035997.18.133e-1624.01SMESG000035997.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30005194
GenBankAFJ24805.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005194 ID=SMED30005194|Name=notchless-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1838bp
CTTTGTATTTAGCTTTCCATGGAATAAAGTTTGTTTAAATATTCGTAATT
AATTTTTCTGTGATGGAATCTATTAATGACACAGTAAGGCATATAAAAGT
CCAGTTTGTAGACTCTGATGGTGTTAATTGTGGAGATGTGTTTCGTATGC
CATCATCATCAAATGTCAAAACATTAAGTAATACTATCAATAAAATTCTT
GAGAAAGAAGGTCCTGAACAATCCATTTATTCTTTTTTTATTACTAATAA
TGTTGAGGATGAAAAAAATGAATTTTCGGGCGGTAATGCTATTGTGACCA
CAATTGAAAATTCTATAATGAATTGTGATAAATTAAAGGCATTTTATGGT
GTGAATGAAACTAAAGAGAATTCAGAGAATTGTGGAATTGAAACTGTTTT
AAGGATTGTATATCATCCACAATCAGTTTTCCGTGTTAGACCAATTACTC
GATGCACAAGTTCAATGCCTGGACATAAAAAGGCAGTACTCGTTGCTCAG
TTTTCTCCAGATGGAAGATATTTGGCAACAGGATCAGCAGATAATACCGT
ACGTTTTTGGGATTTAAATACCCAAACTCCAGAAGCATGTATGGAAAACA
GCCATAAATCTAATGTTTTATTGCTATCTTGGAGTCCAAACGGTAAAGTC
TTGGCATCTTCCAGTGTAACCGGTGAAATAATTCCCTGGTCATTAATTAA
TGGCAAATGGAGTCAGAAAACTTCAGCTTTAATTAAAAAAAATTCTTCTG
CTAAAAAGCTCGTAAAAAGTTTAGCATGGAAACCTTTGCATTTAGATGGT
ACATGCAGACTTTTAGCTGCTGCTTATGATGACAAGACAATAACGATTTG
GGACACAATTTTATGCCAACCAAAAATATCACTTTCGGGTCACATTAAAT
CGGTTCGTTGTATGAAATGGGGAGGTTCGAATTTGATCTATAGTGGTGGG
GAAGATTGTGAAATTCGAGTATGGAGAGCAGAAGATGGAGTTTTGTGCAG
ATCAATGAAACCTCACAGTCATTGGATCAACAGCATAAGCTTGAATACCG
ACTACGCCATTCGCATCGGGGCATTTAATCCCATGTATGAATCGACTAAA
ACCAATATCAACAATTTAGATGCTGAAGAACTTCAAAAACGGGCTCTTTT
GATATACAACACAGCTAGACGGAATGGGGCTGAGAGAATGGTCGTATGTA
GTGATGATAATACCTTGTCGTTGTGGAAACCAGAGGAGTCAAATAAAGCT
ATTTGTGGACGAATGACCGGTCATCAAAACGTGGTCAACGATGTCAAGTT
CTCACCGGATGGATTGTTTATTGCTAGCGCTAGTTTTGATAGGTCGGTAA
AAATTTGGGATGGAATGACAGGAAAATTTCTTGTCAGCCTTCAAGGTCAT
ATGCAAGAAGTTAATTTAATTAGTTGGTCATGTGATAGTCGCTTGCTCGT
GTCTTGCAGCCAAGATTCTTCTATTAAATTGTGGACCATGGCTCAAATTC
GGGGCTTACTAGTTCCGATTGATCAATTGAAGGAAGATATCAGTTTTAAA
AAGAAAATTACTGCTCCGAATTGCAAAAGAGCAAGAATCAGCCATTTGCT
AGAAGCATTGCCTGGACATGCAGATTCTGTGTTTGCGTTAGACTGGAGTC
CTGACGGCATGGTTGTTGCTTCAGGAGGTAAAGATGGGCTCGTCAGATTG
TGGAAATCTTAGTTTTCGAAGATTTTACTGTTAAATATAATTATAAAATC
GTTTTAACTTTTCTCACGGCTGATTGTAGATTATTTTTTATTTATCTATA
AACATTTCATAAAATATAAATCATATTTGGAAAAAAAA
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protein sequence of SMED30005194-orf-1

>SMED30005194-orf-1 ID=SMED30005194-orf-1|Name=SMED30005194-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=550bp
MESINDTVRHIKVQFVDSDGVNCGDVFRMPSSSNVKTLSNTINKILEKEG
PEQSIYSFFITNNVEDEKNEFSGGNAIVTTIENSIMNCDKLKAFYGVNET
KENSENCGIETVLRIVYHPQSVFRVRPITRCTSSMPGHKKAVLVAQFSPD
GRYLATGSADNTVRFWDLNTQTPEACMENSHKSNVLLLSWSPNGKVLASS
SVTGEIIPWSLINGKWSQKTSALIKKNSSAKKLVKSLAWKPLHLDGTCRL
LAAAYDDKTITIWDTILCQPKISLSGHIKSVRCMKWGGSNLIYSGGEDCE
IRVWRAEDGVLCRSMKPHSHWINSISLNTDYAIRIGAFNPMYESTKTNIN
NLDAEELQKRALLIYNTARRNGAERMVVCSDDNTLSLWKPEESNKAICGR
MTGHQNVVNDVKFSPDGLFIASASFDRSVKIWDGMTGKFLVSLQGHMQEV
NLISWSCDSRLLVSCSQDSSIKLWTMAQIRGLLVPIDQLKEDISFKKKIT
APNCKRARISHLLEALPGHADSVFALDWSPDGMVVASGGKDGLVRLWKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036322WD40_repeat_dom_sf
IPR020472G-protein_beta_WD-40_rep
IPR001680WD40_repeat
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR012972NLE
IPR019775WD40_repeat_CS
IPR017986WD40_repeat_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005730nucleolus
Vocabulary: biological process
TermDefinition
GO:0042254ribosome biogenesis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020472G-protein beta WD-40 repeatPRINTSPR00320GPROTEINBRPTcoord: 154..168
score: 40.76
coord: 420..434
score: 38.45
coord: 462..476
score: 33.56
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 267..305
e-value: 2.9E-5
score: 33.5
coord: 436..475
e-value: 1.9E-7
score: 40.7
coord: 170..210
e-value: 0.0042
score: 26.3
coord: 128..167
e-value: 9.3E-11
score: 51.7
coord: 509..548
e-value: 1.3E-8
score: 44.6
coord: 220..264
e-value: 5.6
score: 12.3
coord: 308..389
e-value: 6.2
score: 12.0
coord: 394..433
e-value: 8.1E-10
score: 48.6
IPR001680WD40 repeatPFAMPF00400WD40coord: 401..433
e-value: 2.3E-8
score: 34.5
coord: 269..304
e-value: 1.1E-4
score: 22.9
coord: 130..167
e-value: 5.9E-8
score: 33.3
coord: 515..548
e-value: 2.2E-7
score: 31.4
coord: 438..474
e-value: 1.2E-4
score: 22.8
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 443..476
score: 13.449
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 401..442
score: 16.591
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 178..219
score: 10.508
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 135..170
score: 16.992
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 516..549
score: 16.858
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 274..314
score: 10.408
IPR012972NLEPFAMPF08154NLEcoord: 11..62
e-value: 1.0E-5
score: 25.9
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3DG3DSA:2.130.10.10coord: 131..549
e-value: 8.6E-107
score: 359.3
NoneNo IPR availablePANTHERPTHR19848WD40 REPEAT PROTEINcoord: 5..548
NoneNo IPR availablePANTHERPTHR19848:SF0NOTCHLESS PROTEIN HOMOLOG 1coord: 5..548
NoneNo IPR availableCDDcd00200WD40coord: 131..474
e-value: 2.32922E-65
score: 212.58
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 154..168
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 420..434
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 251..265
IPR017986WD40-repeat-containing domainPROSITEPS50294WD_REPEATS_REGIONcoord: 135..549
score: 56.658
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILYSSF50978WD40 repeat-likecoord: 127..548