SMED30005190

Overview


Name	SMED30005190
Smed ID	SMED30005190
Length (bp)	513

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30005190 (SMED30005190) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005190 (SMED30005190) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005190 (SMED30005190) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005190 (SMED30005190) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005190

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X1 cell	SMED30005190	SMESG000041069.1	SmedASXL_003316	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
pharynx	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30005190	SMESG000041069.1	dd_Smed_v4_237_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gamma neoblast	SMED30005190	SMESG000041069.1	dd_Smed_v6_237_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018
neoblast	SMED30005190	SMESG000041069.1	dd_Smed_v6_237_0	dd_Smed_v6	PMID:29674432 Plass et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30005190 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000065:216248..216854 -	2503

Homology

[Close]

BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 213.001 bits (541), Expect = 1.561e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 161.77 bits (408), Expect = 1.341e-51
Identity = 75/100 (75.00%), Postives = 86/100 (86.00%), Query Frame = -2

Query:  120 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFG 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFG 100

[Close]

BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 201.83 bits (512), Expect = 1.601e-67
Identity = 92/130 (70.77%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 151.369 bits (381), Expect = 5.047e-48
Identity = 67/99 (67.68%), Postives = 84/99 (84.85%), Query Frame = -2

Query:   30 KVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 326
            KVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    3 KVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 101

[Close]

BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073793)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073792)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0300828)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073794)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073795)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Zebrafish
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:336764])

HSP 1 Score: 212.231 bits (539), Expect = 2.334e-71
Identity = 96/130 (73.85%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVL+RP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLLRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVL+RP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLLRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Xenopus
Match: pdp2 (pyruvate dehyrogenase phosphatase catalytic subunit 2 [Source:Xenbase;Acc:XB-GENE-480918])

HSP 1 Score: 214.157 bits (544), Expect = 5.051e-72
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 213.001 bits (541), Expect = 1.071e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 174.096 bits (440), Expect = 1.519e-56
Identity = 80/108 (74.07%), Postives = 94/108 (87.04%), Query Frame = -2

Query:   96 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSA 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSA
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSA 108

[Close]

BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 125.561 bits (314), Expect = 7.616e-38
Identity = 60/78 (76.92%), Postives = 67/78 (85.90%), Query Frame = -2

Query:  186 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPR 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPR
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPR 78

[Close]

BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|P42798|R15A1_ARATH (40S ribosomal protein S15a-1 OS=Arabidopsis thaliana OX=3702 GN=RPS15AA PE=2 SV=2)

HSP 1 Score: 220.32 bits (560), Expect = 1.141e-73
Identity = 102/130 (78.46%), Postives = 117/130 (90.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q9AT34|RS15A_DAUCA (40S ribosomal protein S15a OS=Daucus carota OX=4039 GN=RPS15A PE=2 SV=3)

HSP 1 Score: 220.32 bits (560), Expect = 1.179e-73
Identity = 101/130 (77.69%), Postives = 117/130 (90.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS++NAEKRGK+QV+IRPSSKV+I+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRVSVLNDALKSMFNAEKRGKRQVMIRPSSKVVIKFLMVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q00332|RS15A_BRANA (40S ribosomal protein S15a OS=Brassica napus OX=3708 GN=RPS15A PE=2 SV=3)

HSP 1 Score: 218.779 bits (556), Expect = 3.727e-73
Identity = 101/130 (77.69%), Postives = 117/130 (90.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS++NAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMFNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q9LX88|R15A4_ARATH (40S ribosomal protein S15a-4 OS=Arabidopsis thaliana OX=3702 GN=RPS15AD PE=2 SV=3)

HSP 1 Score: 217.624 bits (553), Expect = 1.466e-72
Identity = 100/130 (76.92%), Postives = 116/130 (89.23%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL D LKS+YNAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFG++V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDGLKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGFIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q6XIM8|RS15A_DROYA (40S ribosomal protein S15a OS=Drosophila yakuba OX=7245 GN=RpS15Aa PE=2 SV=3)

HSP 1 Score: 215.698 bits (548), Expect = 6.509e-72
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. TrEMBL
Match: A0A0V1H872 (40S ribosomal protein S15a (Fragment) OS=Trichinella zimbabwensis OX=268475 GN=RpS15Aa PE=3 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.366e-72
Identity = 105/135 (77.78%), Postives = 115/135 (85.19%), Query Frame = -2

Query:   30 NFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 434
            +F   MVRM VLADAL+SI NAEKRGK+QVLIRPSSKVII+FL VM   GYI EF IVDDHRSGKIVV L GRINKC VISPRFD  +++IEKWT  LLPSRQFGYVV+TTS GIM+H EARRKHLGGKILGYF+
Sbjct:    6 SFSFKMVRMDVLADALRSISNAEKRGKRQVLIRPSSKVIIRFLTVMMQHGYINEFEIVDDHRSGKIVVNLTGRINKCGVISPRFDVGIRDIEKWTEKLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGYFF 140

[Close]

BLAST of SMED30005190 vs. TrEMBL
Match: A0A0V1MS34 (40S ribosomal protein S15a (Fragment) OS=Trichinella papuae OX=268474 GN=RpS15Aa PE=3 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.366e-72
Identity = 105/135 (77.78%), Postives = 115/135 (85.19%), Query Frame = -2

Query:   30 NFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 434
            +F   MVRM VLADAL+SI NAEKRGK+QVLIRPSSKVII+FL VM   GYI EF IVDDHRSGKIVV L GRINKC VISPRFD  +++IEKWT  LLPSRQFGYVV+TTS GIM+H EARRKHLGGKILGYF+
Sbjct:    6 SFSFKMVRMDVLADALRSISNAEKRGKRQVLIRPSSKVIIRFLTVMMQHGYINEFEIVDDHRSGKIVVNLTGRINKCGVISPRFDVGIRDIEKWTEKLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGYFF 140

[Close]

BLAST of SMED30005190 vs. TrEMBL
Match: F6HQY9 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_08s0040g02460 PE=3 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 3.012e-72
Identity = 103/137 (75.18%), Postives = 120/137 (87.59%), Query Frame = -2

Query:   30 LNNFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 440
            L  F   MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVI++FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK+IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:   55 LQPFFARMVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIVKFLLVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKDIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 191

[Close]

BLAST of SMED30005190 vs. TrEMBL
Match: A0A5N6NC77 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_22544 PE=4 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 3.101e-72
Identity = 103/130 (79.23%), Postives = 117/130 (90.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEFVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. TrEMBL
Match: A0A5C7HND3 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_016399 PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 3.537e-72
Identity = 103/130 (79.23%), Postives = 117/130 (90.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEFVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130

[Close]

BLAST of SMED30005190 vs. Ensembl Cavefish
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:336764])

HSP 1 Score: 182.185 bits (461), Expect = 8.217e-60
Identity = 86/130 (66.15%), Postives = 101/130 (77.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK               RP SKVI++FL VM   GYI EF I+DDHR+GKIVV LNGR+NKC VISPRFD  +K++EKW SNLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKCPA------------RPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLNGRLNKCGVISPRFDVQLKDLEKWQSNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 118

[Close]

BLAST of SMED30005190 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010438.1 (pep scaffold:Pmarinus_7.0:GL478779:59925:63803:1 gene:ENSPMAG00000009448.1 transcript:ENSPMAT00000010438.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 214.927 bits (546), Expect = 3.055e-73
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVKFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQIKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Ensembl Yeast
Match: RPS22A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication [Source:SGD;Acc:S000003726])

HSP 1 Score: 208.764 bits (530), Expect = 5.952e-71
Identity = 96/130 (73.85%), Postives = 112/130 (86.15%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            M R SVLADAL +I NAEK GK+QVLIRPSSKVII+FL VMQ  GYI EF  +DDHRSGKIVV+LNGR+NKC VISPRF+  + +IEKWT+NLLP+RQFGYV++TTSAGIM+H EARRKH+ GKILG+ Y
Sbjct:    1 MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY 130

[Close]

BLAST of SMED30005190 vs. Ensembl Yeast
Match: RPS22B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication [Source:SGD;Acc:S000004359])

HSP 1 Score: 207.994 bits (528), Expect = 1.268e-70
Identity = 95/130 (73.08%), Postives = 112/130 (86.15%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            M R SVLADAL +I NAEK GK+QVL+RPSSKVII+FL VMQ  GYI EF  +DDHRSGKIVV+LNGR+NKC VISPRF+  + +IEKWT+NLLP+RQFGYV++TTSAGIM+H EARRKH+ GKILG+ Y
Sbjct:    1 MTRSSVLADALNAINNAEKTGKRQVLLRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY 130

[Close]

BLAST of SMED30005190 vs. Ensembl Nematostella
Match: EDO48339 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJV0])

HSP 1 Score: 206.453 bits (524), Expect = 1.566e-69
Identity = 95/130 (73.08%), Postives = 111/130 (85.38%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR++VL DAL SI NAEKRGK+QV IRPSSKVI++FL VM   GYI EF IVDDHR+GKI+V L GR+NKC VISPRFD  V++IE+W SNLLPSRQFG++V+TTS GIM+H EARRKH GGKILG+FY
Sbjct:    1 MVRVNVLNDALVSICNAEKRGKRQVQIRPSSKVIVKFLTVMMKHGYIGEFVIVDDHRAGKIIVNLTGRLNKCGVISPRFDVGVRDIEQWASNLLPSRQFGFLVLTTSTGIMDHEEARRKHTGGKILGFFY 130

[Close]

BLAST of SMED30005190 vs. Ensembl Medaka
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:101164033])

HSP 1 Score: 213.772 bits (543), Expect = 4.102e-72
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDLQLKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130

[Close]

BLAST of SMED30005190 vs. Planmine SMEST
Match: SMESG000041069.1 (SMESG000041069.1)

HSP 1 Score: 270.011 bits (689), Expect = 1.657e-94
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = -2

Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY
Sbjct:    1 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 130

The following BLAST results are available for this feature:

BLAST of SMED30005190 vs. Ensembl Human
BLAST of SMED30005190 vs. Ensembl Celegans
BLAST of SMED30005190 vs. Ensembl Fly
BLAST of SMED30005190 vs. Ensembl Zebrafish
BLAST of SMED30005190 vs. Ensembl Xenopus
BLAST of SMED30005190 vs. Ensembl Mouse
BLAST of SMED30005190 vs. UniProt/SwissProt
BLAST of SMED30005190 vs. TrEMBL
BLAST of SMED30005190 vs. Ensembl Cavefish
BLAST of SMED30005190 vs. Ensembl Sea Lamprey
BLAST of SMED30005190 vs. Ensembl Yeast
BLAST of SMED30005190 vs. Ensembl Nematostella
BLAST of SMED30005190 vs. Ensembl Medaka
BLAST of SMED30005190 vs. Planmine SMEST

BLAST of SMED30005190 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RPS15A	1.561e-71	74.62	ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN...	[more]
RPS15A	1.561e-71	74.62	ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN...	[more]
RPS15A	1.561e-71	74.62	ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN...	[more]
RPS15A	1.561e-71	74.62	ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN...	[more]
RPS15A	1.341e-51	75.00	ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN...	[more]

BLAST of SMED30005190 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rps-22	1.601e-67	70.77	Ribosomal Protein, Small subunit [Source:UniProtK...	[more]
rps-22	1.601e-67	70.77	Ribosomal Protein, Small subunit [Source:UniProtK...	[more]
rps-22	1.601e-67	70.77	Ribosomal Protein, Small subunit [Source:UniProtK...	[more]
rps-22	1.601e-67	70.77	Ribosomal Protein, Small subunit [Source:UniProtK...	[more]
rps-22	5.047e-48	67.68	Ribosomal Protein, Small subunit [Source:UniProtK...	[more]

BLAST of SMED30005190 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
RpS15Aa	6.493e-73	77.69	gene:FBgn0010198 transcript:FBtr0073793	[more]
RpS15Aa	6.493e-73	77.69	gene:FBgn0010198 transcript:FBtr0073792	[more]
RpS15Aa	6.493e-73	77.69	gene:FBgn0010198 transcript:FBtr0300828	[more]
RpS15Aa	6.493e-73	77.69	gene:FBgn0010198 transcript:FBtr0073794	[more]
RpS15Aa	6.493e-73	77.69	gene:FBgn0010198 transcript:FBtr0073795	[more]

BLAST of SMED30005190 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
rps15a	2.334e-71	73.85	ribosomal protein S15a [Source:NCBI gene;Acc:33676...	[more]
rps15a	2.334e-71	73.85	ribosomal protein S15a [Source:NCBI gene;Acc:33676...	[more]

BLAST of SMED30005190 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pdp2	5.051e-72	75.38	pyruvate dehyrogenase phosphatase catalytic subuni...	[more]

BLAST of SMED30005190 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Rps15a	1.071e-71	74.62	ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:...	[more]
Rps15a	1.071e-71	74.62	ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:...	[more]
Rps15a	1.071e-71	74.62	ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:...	[more]
Rps15a	1.519e-56	74.07	ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:...	[more]
Rps15a	7.616e-38	76.92	ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:...	[more]

BLAST of SMED30005190 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P42798\|R15A1_ARATH	1.141e-73	78.46	40S ribosomal protein S15a-1 OS=Arabidopsis thalia...	[more]
sp\|Q9AT34\|RS15A_DAUCA	1.179e-73	77.69	40S ribosomal protein S15a OS=Daucus carota OX=403...	[more]
sp\|Q00332\|RS15A_BRANA	3.727e-73	77.69	40S ribosomal protein S15a OS=Brassica napus OX=37...	[more]
sp\|Q9LX88\|R15A4_ARATH	1.466e-72	76.92	40S ribosomal protein S15a-4 OS=Arabidopsis thalia...	[more]
sp\|Q6XIM8\|RS15A_DROYA	6.509e-72	77.69	40S ribosomal protein S15a OS=Drosophila yakuba OX...	[more]

BLAST of SMED30005190 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0V1H872	1.366e-72	77.78	40S ribosomal protein S15a (Fragment) OS=Trichinel...	[more]
A0A0V1MS34	1.366e-72	77.78	40S ribosomal protein S15a (Fragment) OS=Trichinel...	[more]
F6HQY9	3.012e-72	75.18	Uncharacterized protein OS=Vitis vinifera OX=29760...	[more]
A0A5N6NC77	3.101e-72	79.23	Uncharacterized protein OS=Mikania micrantha OX=19...	[more]
A0A5C7HND3	3.537e-72	79.23	Uncharacterized protein OS=Acer yangbiense OX=1000...	[more]

BLAST of SMED30005190 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rps15a	8.217e-60	66.15	ribosomal protein S15a [Source:NCBI gene;Acc:33676...	[more]

BLAST of SMED30005190 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000010438.1	3.055e-73	75.38	pep scaffold:Pmarinus_7.0:GL478779:59925:63803:1 g...	[more]

BLAST of SMED30005190 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
RPS22A	5.952e-71	73.85	Protein component of the small (40S) ribosomal sub...	[more]
RPS22B	1.268e-70	73.08	Protein component of the small (40S) ribosomal sub...	[more]

BLAST of SMED30005190 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO48339	1.566e-69	73.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30005190 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rps15a	4.102e-72	75.38	ribosomal protein S15a [Source:NCBI gene;Acc:10116...	[more]

BLAST of SMED30005190 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000041069.1	1.657e-94	100.00	SMESG000041069.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005190 ID=SMED30005190|Name=SMED30005190|organism=Schmidtea mediterranea sexual|type=transcript|length=513bp
ACTGAATCCAAATTTTATTATTATATTTAATAGAAATATCCAAGAATCTT
TCCACCCAGGTGTTTCCTTCTGGCTTCAGTGTGTTCCATAATACCAGCTG
AAGTTGTCATTACAACATATCCAAACTGACGAGATGGAAGAAGGTTGCTG
GTCCATTTTTCGATATTTTTAACAGAAGTATCGAATCTTGGGCTAATAAC
TGCACATTTATTAATTCGGCCGTTCAATTCAACAACAATCTTTCCAGATC
TATGATCGTCAACAATGGTAAACTCTTTAATGTAACCTTTGGATTGCATG
ACCATAAGAAATTGAATGATAACTTTAGAACTTGGTCTAATTAAAACCTG
TTGTTTACCACGTTTTTCGGCGTTATAAATGCTTTTTAAAGCATCAGCTA
GTACTGACATTCGGACCATGTTTAGTATAAAATTATTTAAATTGTAATAC
TTCAAAATGAATAATTTATTTAAACAAATTATTTCATAATTATAACATAA
ATTTTTATGCATG

protein sequence of SMED30005190-orf-1

>SMED30005190-orf-1 ID=SMED30005190-orf-1|Name=SMED30005190-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp
MHKNLCYNYEIICLNKLFILKYYNLNNFILNMVRMSVLADALKSIYNAEK
RGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRI
NKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRK
HLGGKILGYFY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut
PLANA:0000039	gamma neoblast
PLANA:0000429	neoblast
PLANA:0002032	epidermal cell
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR035987	Ribosomal_S8_sf
IPR000630	Ribosomal_S8

Vocabulary: **cellular component**
Term	Definition
GO:0005840	ribosome

Vocabulary: **biological process**
Term	Definition
GO:0006412	translation

Vocabulary: **molecular function**
Term	Definition
GO:0003735	structural constituent of ribosome

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.30.1490.10		coord: 100..160 e-value: 7.1E-32 score: 110.9
None	No IPR available	GENE3D	G3DSA:3.30.1370.30		coord: 31..99 e-value: 4.4E-38 score: 130.9
None	No IPR available	PANTHER	PTHR11758:SF33		coord: 19..160
IPR000630	Ribosomal protein S8	PFAM	PF00410	Ribosomal_S8	coord: 37..160 e-value: 8.8E-23 score: 80.6
IPR000630	Ribosomal protein S8	PANTHER	PTHR11758	40S RIBOSOMAL PROTEIN S15A	coord: 19..160
IPR035987	Ribosomal protein S8 superfamily	SUPERFAMILY	SSF56047	Ribosomal protein S8	coord: 33..160