SMED30005190

Overview
NameSMED30005190
Smed IDSMED30005190
Length (bp)513
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005190 (SMED30005190) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005190 (SMED30005190) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005190 (SMED30005190) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005190 (SMED30005190) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005190

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30005190SMESG000041069.1 SmedASXL_003316SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005190SMESG000041069.1 dd_Smed_v4_237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30005190SMESG000041069.1 dd_Smed_v6_237_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30005190SMESG000041069.1 dd_Smed_v6_237_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005190 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000065:216248..216854 -2503
Homology
BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 213.001 bits (541), Expect = 1.561e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 213.001 bits (541), Expect = 1.561e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 213.001 bits (541), Expect = 1.561e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 213.001 bits (541), Expect = 1.561e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Human
Match: RPS15A (ribosomal protein S15a [Source:HGNC Symbol;Acc:HGNC:10389])

HSP 1 Score: 161.77 bits (408), Expect = 1.341e-51
Identity = 75/100 (75.00%), Postives = 86/100 (86.00%), Query Frame = -2
Query:  120 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFG 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFG 100          
BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 201.83 bits (512), Expect = 1.601e-67
Identity = 92/130 (70.77%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 201.83 bits (512), Expect = 1.601e-67
Identity = 92/130 (70.77%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 201.83 bits (512), Expect = 1.601e-67
Identity = 92/130 (70.77%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 201.83 bits (512), Expect = 1.601e-67
Identity = 92/130 (70.77%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADAL +I NAEKRGK+QVLIRP+SKVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALNAINNAEKRGKRQVLIRPASKVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Celegans
Match: rps-22 (Ribosomal Protein, Small subunit [Source:UniProtKB/TrEMBL;Acc:O17218])

HSP 1 Score: 151.369 bits (381), Expect = 5.047e-48
Identity = 67/99 (67.68%), Postives = 84/99 (84.85%), Query Frame = -2
Query:   30 KVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 326
            KVI++FL VM   GYI EF IVDDHR+GKIVV L GR+NK +VISPR +  + ++EK+T+ LLPSRQFGY+++TTSAGIM+H EARRKHLGGKILG+F+
Sbjct:    3 KVIVRFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKASVISPRLNIRLNDLEKYTNTLLPSRQFGYLILTTSAGIMDHEEARRKHLGGKILGFFF 101          
BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073793)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073792)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0300828)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073794)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Fly
Match: RpS15Aa (gene:FBgn0010198 transcript:FBtr0073795)

HSP 1 Score: 215.698 bits (548), Expect = 6.493e-73
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Zebrafish
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:336764])

HSP 1 Score: 212.231 bits (539), Expect = 2.334e-71
Identity = 96/130 (73.85%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVL+RP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLLRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Zebrafish
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:336764])

HSP 1 Score: 212.231 bits (539), Expect = 2.334e-71
Identity = 96/130 (73.85%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVL+RP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLLRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Xenopus
Match: pdp2 (pyruvate dehyrogenase phosphatase catalytic subunit 2 [Source:Xenbase;Acc:XB-GENE-480918])

HSP 1 Score: 214.157 bits (544), Expect = 5.051e-72
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 213.001 bits (541), Expect = 1.071e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 213.001 bits (541), Expect = 1.071e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 213.001 bits (541), Expect = 1.071e-71
Identity = 97/130 (74.62%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 174.096 bits (440), Expect = 1.519e-56
Identity = 80/108 (74.07%), Postives = 94/108 (87.04%), Query Frame = -2
Query:   96 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSA 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFG++V+TTSA
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSA 108          
BLAST of SMED30005190 vs. Ensembl Mouse
Match: Rps15a (ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:2389091])

HSP 1 Score: 125.561 bits (314), Expect = 7.616e-38
Identity = 60/78 (76.92%), Postives = 67/78 (85.90%), Query Frame = -2
Query:  186 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPR 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPR
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPR 78          
BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|P42798|R15A1_ARATH (40S ribosomal protein S15a-1 OS=Arabidopsis thaliana OX=3702 GN=RPS15AA PE=2 SV=2)

HSP 1 Score: 220.32 bits (560), Expect = 1.141e-73
Identity = 102/130 (78.46%), Postives = 117/130 (90.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q9AT34|RS15A_DAUCA (40S ribosomal protein S15a OS=Daucus carota OX=4039 GN=RPS15A PE=2 SV=3)

HSP 1 Score: 220.32 bits (560), Expect = 1.179e-73
Identity = 101/130 (77.69%), Postives = 117/130 (90.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS++NAEKRGK+QV+IRPSSKV+I+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRVSVLNDALKSMFNAEKRGKRQVMIRPSSKVVIKFLMVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q00332|RS15A_BRANA (40S ribosomal protein S15a OS=Brassica napus OX=3708 GN=RPS15A PE=2 SV=3)

HSP 1 Score: 218.779 bits (556), Expect = 3.727e-73
Identity = 101/130 (77.69%), Postives = 117/130 (90.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS++NAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMFNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q9LX88|R15A4_ARATH (40S ribosomal protein S15a-4 OS=Arabidopsis thaliana OX=3702 GN=RPS15AD PE=2 SV=3)

HSP 1 Score: 217.624 bits (553), Expect = 1.466e-72
Identity = 100/130 (76.92%), Postives = 116/130 (89.23%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL D LKS+YNAEKRGK+QV+IRPSSKVII+FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFG++V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDGLKSMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEGWTARLLPSRQFGFIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. UniProt/SwissProt
Match: sp|Q6XIM8|RS15A_DROYA (40S ribosomal protein S15a OS=Drosophila yakuba OX=7245 GN=RpS15Aa PE=2 SV=3)

HSP 1 Score: 215.698 bits (548), Expect = 6.509e-72
Identity = 101/130 (77.69%), Postives = 113/130 (86.92%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK I NAEKRGK+QVL+RP SKVII+FL VM   GYI EF IVDDHRSGKIVV L GR+NKC VISPRFD  + +IEKWT+NLLPSRQFGYVV+TTS GIM+H EARRKHLGGKILG+F+
Sbjct:    1 MVRMNVLADALKCINNAEKRGKRQVLLRPCSKVIIKFLTVMMKHGYIGEFEIVDDHRSGKIVVNLTGRLNKCGVISPRFDVPINDIEKWTNNLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGFFF 130          
BLAST of SMED30005190 vs. TrEMBL
Match: A0A0V1H872 (40S ribosomal protein S15a (Fragment) OS=Trichinella zimbabwensis OX=268475 GN=RpS15Aa PE=3 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.366e-72
Identity = 105/135 (77.78%), Postives = 115/135 (85.19%), Query Frame = -2
Query:   30 NFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 434
            +F   MVRM VLADAL+SI NAEKRGK+QVLIRPSSKVII+FL VM   GYI EF IVDDHRSGKIVV L GRINKC VISPRFD  +++IEKWT  LLPSRQFGYVV+TTS GIM+H EARRKHLGGKILGYF+
Sbjct:    6 SFSFKMVRMDVLADALRSISNAEKRGKRQVLIRPSSKVIIRFLTVMMQHGYINEFEIVDDHRSGKIVVNLTGRINKCGVISPRFDVGIRDIEKWTEKLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGYFF 140          
BLAST of SMED30005190 vs. TrEMBL
Match: A0A0V1MS34 (40S ribosomal protein S15a (Fragment) OS=Trichinella papuae OX=268474 GN=RpS15Aa PE=3 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.366e-72
Identity = 105/135 (77.78%), Postives = 115/135 (85.19%), Query Frame = -2
Query:   30 NFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 434
            +F   MVRM VLADAL+SI NAEKRGK+QVLIRPSSKVII+FL VM   GYI EF IVDDHRSGKIVV L GRINKC VISPRFD  +++IEKWT  LLPSRQFGYVV+TTS GIM+H EARRKHLGGKILGYF+
Sbjct:    6 SFSFKMVRMDVLADALRSISNAEKRGKRQVLIRPSSKVIIRFLTVMMQHGYINEFEIVDDHRSGKIVVNLTGRINKCGVISPRFDVGIRDIEKWTEKLLPSRQFGYVVLTTSGGIMDHEEARRKHLGGKILGYFF 140          
BLAST of SMED30005190 vs. TrEMBL
Match: F6HQY9 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_08s0040g02460 PE=3 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 3.012e-72
Identity = 103/137 (75.18%), Postives = 120/137 (87.59%), Query Frame = -2
Query:   30 LNNFILNMVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 440
            L  F   MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVI++FL+VMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK+IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:   55 LQPFFARMVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIVKFLLVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKDIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 191          
BLAST of SMED30005190 vs. TrEMBL
Match: A0A5N6NC77 (Uncharacterized protein OS=Mikania micrantha OX=192012 GN=E3N88_22544 PE=4 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 3.101e-72
Identity = 103/130 (79.23%), Postives = 117/130 (90.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRISVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEFVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. TrEMBL
Match: A0A5C7HND3 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_016399 PE=3 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 3.537e-72
Identity = 103/130 (79.23%), Postives = 117/130 (90.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR+SVL DALKS+YNAEKRGK+QV+IRPSSKVII+FLMVMQ  GYI EF  VDDHRSGKIVVELNGR+NKC VISPRFD  VK IE WT+ LLPSRQFGY+V+TTSAGIM+H EARRK++GGK+LG+FY
Sbjct:    1 MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEFVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY 130          
BLAST of SMED30005190 vs. Ensembl Cavefish
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:336764])

HSP 1 Score: 182.185 bits (461), Expect = 8.217e-60
Identity = 86/130 (66.15%), Postives = 101/130 (77.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALK               RP SKVI++FL VM   GYI EF I+DDHR+GKIVV LNGR+NKC VISPRFD  +K++EKW SNLLPSRQFG++V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKCPA------------RPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLNGRLNKCGVISPRFDVQLKDLEKWQSNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF 118          
BLAST of SMED30005190 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010438.1 (pep scaffold:Pmarinus_7.0:GL478779:59925:63803:1 gene:ENSPMAG00000009448.1 transcript:ENSPMAT00000010438.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 214.927 bits (546), Expect = 3.055e-73
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVKFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQIKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Ensembl Yeast
Match: RPS22A (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication [Source:SGD;Acc:S000003726])

HSP 1 Score: 208.764 bits (530), Expect = 5.952e-71
Identity = 96/130 (73.85%), Postives = 112/130 (86.15%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            M R SVLADAL +I NAEK GK+QVLIRPSSKVII+FL VMQ  GYI EF  +DDHRSGKIVV+LNGR+NKC VISPRF+  + +IEKWT+NLLP+RQFGYV++TTSAGIM+H EARRKH+ GKILG+ Y
Sbjct:    1 MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY 130          
BLAST of SMED30005190 vs. Ensembl Yeast
Match: RPS22B (Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication [Source:SGD;Acc:S000004359])

HSP 1 Score: 207.994 bits (528), Expect = 1.268e-70
Identity = 95/130 (73.08%), Postives = 112/130 (86.15%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            M R SVLADAL +I NAEK GK+QVL+RPSSKVII+FL VMQ  GYI EF  +DDHRSGKIVV+LNGR+NKC VISPRF+  + +IEKWT+NLLP+RQFGYV++TTSAGIM+H EARRKH+ GKILG+ Y
Sbjct:    1 MTRSSVLADALNAINNAEKTGKRQVLLRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY 130          
BLAST of SMED30005190 vs. Ensembl Nematostella
Match: EDO48339 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJV0])

HSP 1 Score: 206.453 bits (524), Expect = 1.566e-69
Identity = 95/130 (73.08%), Postives = 111/130 (85.38%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVR++VL DAL SI NAEKRGK+QV IRPSSKVI++FL VM   GYI EF IVDDHR+GKI+V L GR+NKC VISPRFD  V++IE+W SNLLPSRQFG++V+TTS GIM+H EARRKH GGKILG+FY
Sbjct:    1 MVRVNVLNDALVSICNAEKRGKRQVQIRPSSKVIVKFLTVMMKHGYIGEFVIVDDHRAGKIIVNLTGRLNKCGVISPRFDVGVRDIEQWASNLLPSRQFGFLVLTTSTGIMDHEEARRKHTGGKILGFFY 130          
BLAST of SMED30005190 vs. Ensembl Medaka
Match: rps15a (ribosomal protein S15a [Source:NCBI gene;Acc:101164033])

HSP 1 Score: 213.772 bits (543), Expect = 4.102e-72
Identity = 98/130 (75.38%), Postives = 114/130 (87.69%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRM+VLADALKSI NAEKRGK+QVLIRP SKVI++FL VM   GYI EF I+DDHR+GKIVV L GR+NKC VISPRFD  +K++EKW +NLLPSRQFGY+V+TTSAGIM+H EARRKH GGKILG+F+
Sbjct:    1 MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDLQLKDLEKWQNNLLPSRQFGYIVLTTSAGIMDHEEARRKHTGGKILGFFF 130          
BLAST of SMED30005190 vs. Planmine SMEST
Match: SMESG000041069.1 (SMESG000041069.1)

HSP 1 Score: 270.011 bits (689), Expect = 1.657e-94
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = -2
Query:   30 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 419
            MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY
Sbjct:    1 MVRMSVLADALKSIYNAEKRGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRINKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRKHLGGKILGYFY 130          
The following BLAST results are available for this feature:
BLAST of SMED30005190 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPS15A1.561e-7174.62ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN... [more]
RPS15A1.561e-7174.62ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN... [more]
RPS15A1.561e-7174.62ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN... [more]
RPS15A1.561e-7174.62ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN... [more]
RPS15A1.341e-5175.00ribosomal protein S15a [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of SMED30005190 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rps-221.601e-6770.77Ribosomal Protein, Small subunit [Source:UniProtK... [more]
rps-221.601e-6770.77Ribosomal Protein, Small subunit [Source:UniProtK... [more]
rps-221.601e-6770.77Ribosomal Protein, Small subunit [Source:UniProtK... [more]
rps-221.601e-6770.77Ribosomal Protein, Small subunit [Source:UniProtK... [more]
rps-225.047e-4867.68Ribosomal Protein, Small subunit [Source:UniProtK... [more]
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BLAST of SMED30005190 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RpS15Aa6.493e-7377.69gene:FBgn0010198 transcript:FBtr0073793[more]
RpS15Aa6.493e-7377.69gene:FBgn0010198 transcript:FBtr0073792[more]
RpS15Aa6.493e-7377.69gene:FBgn0010198 transcript:FBtr0300828[more]
RpS15Aa6.493e-7377.69gene:FBgn0010198 transcript:FBtr0073794[more]
RpS15Aa6.493e-7377.69gene:FBgn0010198 transcript:FBtr0073795[more]
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BLAST of SMED30005190 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps15a2.334e-7173.85ribosomal protein S15a [Source:NCBI gene;Acc:33676... [more]
rps15a2.334e-7173.85ribosomal protein S15a [Source:NCBI gene;Acc:33676... [more]
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BLAST of SMED30005190 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pdp25.051e-7275.38pyruvate dehyrogenase phosphatase catalytic subuni... [more]
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BLAST of SMED30005190 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rps15a1.071e-7174.62ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:... [more]
Rps15a1.071e-7174.62ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:... [more]
Rps15a1.071e-7174.62ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:... [more]
Rps15a1.519e-5674.07ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:... [more]
Rps15a7.616e-3876.92ribosomal protein S15A [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of SMED30005190 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P42798|R15A1_ARATH1.141e-7378.4640S ribosomal protein S15a-1 OS=Arabidopsis thalia... [more]
sp|Q9AT34|RS15A_DAUCA1.179e-7377.6940S ribosomal protein S15a OS=Daucus carota OX=403... [more]
sp|Q00332|RS15A_BRANA3.727e-7377.6940S ribosomal protein S15a OS=Brassica napus OX=37... [more]
sp|Q9LX88|R15A4_ARATH1.466e-7276.9240S ribosomal protein S15a-4 OS=Arabidopsis thalia... [more]
sp|Q6XIM8|RS15A_DROYA6.509e-7277.6940S ribosomal protein S15a OS=Drosophila yakuba OX... [more]
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BLAST of SMED30005190 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0V1H8721.366e-7277.7840S ribosomal protein S15a (Fragment) OS=Trichinel... [more]
A0A0V1MS341.366e-7277.7840S ribosomal protein S15a (Fragment) OS=Trichinel... [more]
F6HQY93.012e-7275.18Uncharacterized protein OS=Vitis vinifera OX=29760... [more]
A0A5N6NC773.101e-7279.23Uncharacterized protein OS=Mikania micrantha OX=19... [more]
A0A5C7HND33.537e-7279.23Uncharacterized protein OS=Acer yangbiense OX=1000... [more]
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BLAST of SMED30005190 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps15a8.217e-6066.15ribosomal protein S15a [Source:NCBI gene;Acc:33676... [more]
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BLAST of SMED30005190 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000010438.13.055e-7375.38pep scaffold:Pmarinus_7.0:GL478779:59925:63803:1 g... [more]
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BLAST of SMED30005190 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS22A5.952e-7173.85Protein component of the small (40S) ribosomal sub... [more]
RPS22B1.268e-7073.08Protein component of the small (40S) ribosomal sub... [more]
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BLAST of SMED30005190 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO483391.566e-6973.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30005190 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps15a4.102e-7275.38ribosomal protein S15a [Source:NCBI gene;Acc:10116... [more]
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BLAST of SMED30005190 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000041069.11.657e-94100.00SMESG000041069.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005190 ID=SMED30005190|Name=SMED30005190|organism=Schmidtea mediterranea sexual|type=transcript|length=513bp
ACTGAATCCAAATTTTATTATTATATTTAATAGAAATATCCAAGAATCTT
TCCACCCAGGTGTTTCCTTCTGGCTTCAGTGTGTTCCATAATACCAGCTG
AAGTTGTCATTACAACATATCCAAACTGACGAGATGGAAGAAGGTTGCTG
GTCCATTTTTCGATATTTTTAACAGAAGTATCGAATCTTGGGCTAATAAC
TGCACATTTATTAATTCGGCCGTTCAATTCAACAACAATCTTTCCAGATC
TATGATCGTCAACAATGGTAAACTCTTTAATGTAACCTTTGGATTGCATG
ACCATAAGAAATTGAATGATAACTTTAGAACTTGGTCTAATTAAAACCTG
TTGTTTACCACGTTTTTCGGCGTTATAAATGCTTTTTAAAGCATCAGCTA
GTACTGACATTCGGACCATGTTTAGTATAAAATTATTTAAATTGTAATAC
TTCAAAATGAATAATTTATTTAAACAAATTATTTCATAATTATAACATAA
ATTTTTATGCATG
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protein sequence of SMED30005190-orf-1

>SMED30005190-orf-1 ID=SMED30005190-orf-1|Name=SMED30005190-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp
MHKNLCYNYEIICLNKLFILKYYNLNNFILNMVRMSVLADALKSIYNAEK
RGKQQVLIRPSSKVIIQFLMVMQSKGYIKEFTIVDDHRSGKIVVELNGRI
NKCAVISPRFDTSVKNIEKWTSNLLPSRQFGYVVMTTSAGIMEHTEARRK
HLGGKILGYFY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR035987Ribosomal_S8_sf
IPR000630Ribosomal_S8
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.1490.10coord: 100..160
e-value: 7.1E-32
score: 110.9
NoneNo IPR availableGENE3DG3DSA:3.30.1370.30coord: 31..99
e-value: 4.4E-38
score: 130.9
NoneNo IPR availablePANTHERPTHR11758:SF33coord: 19..160
IPR000630Ribosomal protein S8PFAMPF00410Ribosomal_S8coord: 37..160
e-value: 8.8E-23
score: 80.6
IPR000630Ribosomal protein S8PANTHERPTHR1175840S RIBOSOMAL PROTEIN S15Acoord: 19..160
IPR035987Ribosomal protein S8 superfamilySUPERFAMILYSSF56047Ribosomal protein S8coord: 33..160