SMED30005172
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005172 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30005172 aligns in the following genomic locations:
Homology
BLAST of SMED30005172 vs. TrEMBL
Match: A0A1I8GV57 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 95.9005 bits (237), Expect = 8.882e-19 Identity = 67/232 (28.88%), Postives = 104/232 (44.83%), Query Frame = 1 Query: 112 MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESL---------WSGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYI----QRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDEF 768 MK+LK+F+ F+ + VY + F E+ R+++R S+ L + L S W WSGLWD CFL + NK H++ H+ E+ Q+A+IG L + IGS+L++ G ++ +K YY+HSA E F VS A + V +YP ++ ++ + GYGY L W+ A +L MMLD+ Sbjct: 97 MKSLKNFLSFKVPDNSAYEQRVY----TEDFCFQEKRRLRWRCSGAGASVLALILYANMLHPASHWTAGPAGGGGGEGSGRGSAWSGLWDACFL------------LAFNKTVPHELQ--HISCEHFVPDWQKAIIGLLFFSVLFAAIGSVLAVCGLCAGHLPRKMYYYHSAGEIFFVSAVATTIAVGVYPYVVQRFV--YQSASYGYGYGLGWVTAGLLYLASLSMMLDDL 308
BLAST of SMED30005172 vs. TrEMBL
Match: A0A1I8IT32 (VWFA domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 92.4337 bits (228), Expect = 9.047e-17 Identity = 64/228 (28.07%), Postives = 99/228 (43.42%), Query Frame = 1 Query: 124 KSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESL---------WSGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYI----QRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDEF 768 K+F+ F+ + VY + F E+ R+++R S+ L + L S W WS LWD CFL + NK H++ HV E+ Q+A+IG L + IGS+L++ G ++ +K YY+HSA E F VS A + V +YP ++ ++ + GYGY L W+ A +L MMLD+ Sbjct: 1461 KNFLSFKVPDNSAYEQRVY----TEDFCFQEKRRLRWRCSGAGASVLALILYANMLHPASHWTAGPAGGGGGEGSGRGSAWSSLWDACFL------------LAFNKTVPHELQ--HVSCEHFVPDWQKAIIGLLFFSVLFAAIGSVLAVCGLCAGHLPRKMYYYHSAGEIFFVSAVATTIAVGVYPYVVQRFVYQSASY--GYGYGLGWVTAGLLYLASLSMMLDDL 1668
BLAST of SMED30005172 vs. TrEMBL
Match: A0A1I8HVH0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 2.120e-10 Identity = 42/141 (29.79%), Postives = 66/141 (46.81%), Query Frame = 1 Query: 340 SGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLD 762 SGLWD CF +++ + +D + S+ + + + VIGS++A + GF + + +K YYFHS+ E F V + +IYP S++ +L + GYGY L W + L F MMLD Sbjct: 189 SGLWDTCFTANDTCISAFDLPTTNSDASDWQKSVAFLLVSSVLLTVIGSVVA------------LCGFWVSYLPRKIYYFHSSGEIFFVCAMLSTISAVIYPYSLQRFL--LHSIVYGYGYFLGWATSGLQCLAAFCMMLD 315
BLAST of SMED30005172 vs. TrEMBL
Match: A0A1I8IF80 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 2.943e-10 Identity = 42/141 (29.79%), Postives = 66/141 (46.81%), Query Frame = 1 Query: 340 SGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLD 762 SGLWD CF +++ + +D + S+ + + + VIGS++A + GF + + +K YYFHS+ E F V + +IYP S++ +L + GYGY L W + L F MMLD Sbjct: 221 SGLWDTCFTANDTCISAFDLPTTNSDASDWQKSVAFLLVSSVLLTVIGSVVA------------LCGFWVSYLPRKIYYFHSSGEIFFVCAMLSTISAVIYPYSLQRFL--LHSIVYGYGYFLGWATSGLQCLAAFCMMLD 347
BLAST of SMED30005172 vs. TrEMBL
Match: A0A267EMJ4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029213g1 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 2.996e-10 Identity = 42/141 (29.79%), Postives = 66/141 (46.81%), Query Frame = 1 Query: 340 SGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLD 762 SGLWD CF +++ + +D + S+ + + + VIGS++A + GF + + +K YYFHS+ E F V + +IYP S++ +L + GYGY L W + L F MMLD Sbjct: 223 SGLWDTCFTANDTCISAFDLPTTNSDASDWQKSVAFLLVSSVLLTVIGSVVA------------LCGFWVSYLPRKIYYFHSSGEIFFVCAMLSTISAVIYPYSLQRFL--LHSIVYGYGYFLGWATSGLQCLAAFCMMLD 349
BLAST of SMED30005172 vs. Planmine SMEST
Match: SMESG000018557.1 (SMESG000018557.1) HSP 1 Score: 503.056 bits (1294), Expect = 0.000e+0 Identity = 266/268 (99.25%), Postives = 267/268 (99.63%), Query Frame = 1 Query: 112 MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESLWSGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDEFCXXXXXXXXXXXXXXXXXXTYTLNKSKTNTKNSIQNNKESIQTNKENWV 915 MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESLWSGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNN+FKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFL FFIMMLDEFCSASTIKTSSISGSSSNAKTYTLNKSKTNTKNSIQNNKESIQTNKENWV Sbjct: 1 MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESLWSGLWDLCFLSSNFTFVGAHAQMKENKYNDHKMSICHVKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNLFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLDFFIMMLDEFCSASTIKTSSISGSSSNAKTYTLNKSKTNTKNSIQNNKESIQTNKENWV 268
BLAST of SMED30005172 vs. Planmine SMEST
Match: SMESG000069934.1 (SMESG000069934.1) HSP 1 Score: 54.299 bits (129), Expect = 3.443e-8 Identity = 46/169 (27.22%), Postives = 82/169 (48.52%), Query Frame = 1 Query: 112 MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSISNLAMIIVSLQFQSQWVKRIGNESLWSGLWDLCFLSSNFTFVGAHAQMKENKY-NDHKMSICH-VKIEYIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSG 612 MK++ F+ + + +Y +D F + E R +R CA L + + A++I L ++ W+K G+ ++ LW C + G+ + K +KY +D CH I+ +G +L + I +LL++ G I N+ KK YYF+S+ E +LV+ Sbjct: 66 MKSMSILSVFKCPDDRSYSRRIY----SDEFQMAEIERKPFRICAFFLGLFSDALLITVLLPETPWLKEHGSSYIYC-LWRSC---EDIVLNGSTSHDKSHKYESDIAGHHCHGFMID------VGLILFSLFFTTIATLLNLMGIIVKNLPKKMYYFNSSYEMYLVTA 220
BLAST of SMED30005172 vs. Planmine SMEST
Match: SMESG000029557.1 (SMESG000029557.1) HSP 1 Score: 51.9878 bits (123), Expect = 2.494e-7 Identity = 29/113 (25.66%), Postives = 57/113 (50.44%), Query Frame = 1 Query: 439 ICHVKIE---YIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDEF 768 C + I+ +++ + S++ + C+ L+ + +G ++ KK YY+HS +EFF V+ + ++I+ + ++ + F GY Y L WI F ++ LDEF Sbjct: 239 TCSLAIQTPNWLKSVIFLSIIGSLCN-LLATFFGFSGVFVKHLPKKLYYYHSGSEFFCVASLSYGSGLLIFTLFSKNQFNKFTWFRYGYAYTLGWIGVLLSMTAFILLKLDEF 350
BLAST of SMED30005172 vs. Planmine SMEST
Match: SMESG000074883.1 (SMESG000074883.1) HSP 1 Score: 50.447 bits (119), Expect = 5.914e-7 Identity = 30/99 (30.30%), Postives = 55/99 (55.56%), Query Frame = 1 Query: 469 RAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDE 765 + +IG + ++ +GL G +++I G I I K+ YY+HS+ EF +++ I++ ++I+ IS R L G + +C YFL F ++ +DE Sbjct: 170 KLLIGFICLSSLTGLCGLIVAILGCIHKPIHKQMYYYHSSGEFSVIAFAMIMVGLLIFFISDRRTLKFNS---FGLSFWMCLACGFLYFLAFILLTVDE 265
BLAST of SMED30005172 vs. Planmine SMEST
Match: SMESG000055359.1 (SMESG000055359.1) HSP 1 Score: 50.8322 bits (120), Expect = 6.574e-7 Identity = 28/113 (24.78%), Postives = 57/113 (50.44%), Query Frame = 1 Query: 439 ICHVKIE---YIQRAVIGSLLAATCSGLIGSLLSIAGFITNNIFKKYYYFHSAAEFFLVSGFAILLVVIIYPISIRHYLDPKEKFILGYGYILCWIAAANYFLGFFIMMLDEF 768 C + I+ +++ + S++ + C+ L+ + +G ++ KK YY+HS +EF+ V+ + ++I+ + ++ + F GY Y L WI F ++ LDEF Sbjct: 239 TCSLAIQTPNWLKSVIFLSIIGSLCN-LLATFFGFSGVFVKHLPKKLYYYHSGSEFYCVASLSYGSGLLIFTLFSKNQFNKFTWFRYGYAYTLGWIGVLLSMTAFILLKLDEF 350 The following BLAST results are available for this feature:
BLAST of SMED30005172 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30005172 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30005172 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30005172 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30005172 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30005172 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005172 ID=SMED30005172|Name=SMED30005172|organism=Schmidtea mediterranea sexual|type=transcript|length=960bpback to top protein sequence of SMED30005172-orf-1 >SMED30005172-orf-1 ID=SMED30005172-orf-1|Name=SMED30005172-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=269bp MKNLKSFIKFRNLNQKPKFHEVYLSKINDAFIILEEIRMKYRKCAMILSIback to top Annotated Terms
The following terms have been associated with this transcript:
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