SMED30005171

Overview
NameSMED30005171
Smed IDSMED30005171
Length (bp)1009
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005171 (SMED30005171) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005171 (SMED30005171) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005171 (SMED30005171) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005171 (SMED30005171) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005171

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 25

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30005171SMESG000024345.1 SMED30005171smed_20140614PMID:29906446
Zeng et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
X1 cellSMED30005171SMESG000024345.1 SmedASXL_010308SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30005171SMESG000024345.1 SmedASXL_010308SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005171SMESG000024345.1 Contig12011uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005171SMESG000024345.1 Contig12011newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005171SMESG000024345.1 Contig38869uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005171SMESG000024345.1 Contig38869newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
neoblastSMED30005171SMESG000024345.1 dd_Smed_v6_629_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30005171SMESG000024345.1 Contig225GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30005171SMESG000024345.1 Contig225GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
zeta neoblastSMED30005171SMESG000024345.1 dd_Smed_v4_629_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30005171SMESG000024345.1 SMED_01456_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30005171SMESG000024345.1 SMED_01456_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005171 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001315:72242..77890 +4775
Homology
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BLAST of SMED30005171 vs. Planmine SMEST
Match: SMESG000024345.1 (SMESG000024345.1)

HSP 1 Score: 187.578 bits (475), Expect = 8.822e-59
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 1 
Query:   22 MPRKNKETAVKKXXXXXXXXXXXXXXXXXXPKSKIKEESPVKTPKRQAAKRASNEVRKTMADEENVNSNVESIPSVEKVDQDGDKNSAEDNKAXXXXXXXXXXXXXXXXXIVDEESTELVIDTPNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNHVNNIESNDKVETLGS 546
            MPRKNKETAVKKEEAPELSRRRSARLAAVEPKSKIKEESPVKTPKRQAAKRASNEVRKTMADEENVNSNVESIPSVEKVDQDGDKNSAEDNKAETETKKLKLDEEDKLTKIVDEESTELVIDTPNEKKEVKEDSIKDKSDSEDKGSKSAEESGKGSEQNHVNNIESNDKVETLGS
Sbjct:    1 MPRKNKETAVKKEEAPELSRRRSARLAAVEPKSKIKEESPVKTPKRQAAKRASNEVRKTMADEENVNSNVESIPSVEKVDQDGDKNSAEDNKAETETKKLKLDEEDKLTKIVDEESTELVIDTPNEKKEVKEDSIKDKSDSEDKGSKSAEESGKGSEQNHVNNIESNDKVETLGS 175
The following BLAST results are available for this feature:
BLAST of SMED30005171 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005171 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000024345.18.822e-59100.00SMESG000024345.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005171 ID=SMED30005171|Name=SMED30005171|organism=Schmidtea mediterranea sexual|type=transcript|length=1009bp
TCTATATAAATCTTATATGATATGCCTCGTAAAAATAAGGAAACTGCAGT
GAAAAAGGAAGAAGCTCCAGAATTAAGTCGTCGTAGAAGTGCTAGATTGG
CAGCAGTTGAACCCAAATCTAAAATAAAAGAGGAAAGCCCGGTTAAGACA
CCCAAACGGCAAGCAGCTAAAAGAGCTTCGAATGAAGTGAGAAAAACAAT
GGCCGACGAAGAGAATGTTAATTCAAATGTTGAATCAATTCCTTCTGTAG
AAAAGGTTGACCAAGATGGCGACAAAAATTCAGCAGAAGATAATAAAGCT
GAAACAGAAACAAAAAAGTTAAAATTAGACGAAGAAGATAAATTAACAAA
AATTGTTGATGAGGAATCAACTGAATTAGTTATTGACACACCTAACGAAA
AGAAAGAAGTTAAGGAGGATAGCATTAAAGATAAAAGTGATTCGGAGGAT
AAAGGTTCAAAGAGTGCTGAAGAATCTGGCAAAGGTTCTGAACAGAATCA
TGTTAATAATATCGAATCAAATGATAAAGTAGAAACTCTCGGCAGTTAAA
TTAAAATATAATTGTCTGGTTTAAATGTTTGATATATTTTATCATTTATT
ATGTATTGTCATTGTGAATATTCATATTTAAATGGTTTTGGTGCTTAATA
AATTAATGCATGCAATTAAATTCGCACCAATTATGCATTTGAAGTATTTA
TTTGTGAAAAATGGTTTGTTATGTTGGTTTTAAATGTAATTAAAATGGAT
TGTACACAGTGGTGTTATAACTTGTATTATAAATTATATTTAATTTGTGT
AGAAAATAAATAATGTGGATTTTAATTTTTCAATAAATTTTCATGAATTA
TCTTTTCCAAGTTTCAATTTGTTATGAGATGGATATTTAGTTAAGCGGCT
GTCAATTTTATATGAATGGATAAACCAGTTGTCAAGGCCGGTGCCAGAGT
ACCTGTTGAATTGTGCAACTAATTATGTAATTAATATTAACTTTGATTTC
TAATCCGTC
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protein sequence of SMED30005171-orf-1

>SMED30005171-orf-1 ID=SMED30005171-orf-1|Name=SMED30005171-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp
MPRKNKETAVKKEEAPELSRRRSARLAAVEPKSKIKEESPVKTPKRQAAK
RASNEVRKTMADEENVNSNVESIPSVEKVDQDGDKNSAEDNKAETETKKL
KLDEEDKLTKIVDEESTELVIDTPNEKKEVKEDSIKDKSDSEDKGSKSAE
ESGKGSEQNHVNNIESNDKVETLGS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000231vitelline gland
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 88..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..178
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..178