SMED30005154

Overview
NameSMED30005154
Smed IDSMED30005154
Length (bp)2469
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005154 (SMED30005154) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005154 (SMED30005154) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005154 (SMED30005154) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005154 (SMED30005154) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30005154

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
tail regionSMED30005154h1SMcG0009176 SmedASXL_018940SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30005154h1SMcG0006034 Contig103GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30005154h1SMcG0009176 Contig103GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal cellSMED30005154h1SMcG0009176 dd_Smed_v4_2225_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30005154 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002298:29976..34736 +12115
Homology
[Close]
BLAST of SMED30005154 vs. Ensembl Human
Match: MRPL20 (mitochondrial ribosomal protein L20 [Source:HGNC Symbol;Acc:HGNC:14478])

HSP 1 Score: 65.4698 bits (158), Expect = 7.296e-12
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = -1 
Query:  802 DRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSL 1107
            DR    ++ ++ A +F  R NRCY+L ++ VI +F     AR+L++   +  +  RI     EH L  P + I +L    +E+N+ +L  L IYEP++FKSL
Sbjct:   16 DRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPAL-IGNLVKCQVELNRKVLADLAIYEPKTFKSL 116
[Close]
BLAST of SMED30005154 vs. Ensembl Celegans
Match: mrpl-20 (Mitochondrial Ribosomal Protein, Large [Source:UniProtKB/TrEMBL;Acc:Q9NAG2])

HSP 1 Score: 54.6842 bits (130), Expect = 3.550e-8
Identity = 44/134 (32.84%), Postives = 59/134 (44.03%), Query Frame = -1 
Query:  784 MFLSFLLLTRRVIMK--------PKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1161
            M LS  L  RRV+          PKPD  + RE+  RF            K   +K    F ++N  R   RN  K +  ER+     EHD+   + F   L   +I ++   L  L IYEPRSF+SLV   K+
Sbjct:    1 MQLSKALSLRRVLNSGFHPFQVIPKPDVWMKRERLNRFTAWQYASERSTVKGAYRKEDKIFAYLNMQRQDERNLEKFHAEERVRTALEEHDMEYSK-FKSILSQSHILLDNICLSQLAIYEPRSFRSLVAFAKE 133
[Close]
BLAST of SMED30005154 vs. Ensembl Fly
Match: mRpL20 (gene:FBgn0036335 transcript:FBtr0075897)

HSP 1 Score: 60.4622 bits (145), Expect = 2.064e-10
Identity = 36/126 (28.57%), Postives = 63/126 (50.00%), Query Frame = -1 
Query:  784 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1161
            +FL+  LL R       PD    + +  + A ++  R    Y   I+ V  +  +    R L++      ++ R+E GC ++ +G  E F E L   +I +NK +L  L I+EPRSF++LV + ++
Sbjct:    2 VFLNLPLLVR----SRGPDEFWRKRRIFKLAAHYRSRTRNVYSFAIRSVHRALAYATKGRKLKKLDMAQLWSTRVEAGCQQYGVGL-ETFKEGLARSDILLNKKVLSDLAIWEPRSFEALVKISRE 122
[Close]
BLAST of SMED30005154 vs. Ensembl Zebrafish
Match: mrpl20 (mitochondrial ribosomal protein L20 [Source:ZFIN;Acc:ZDB-GENE-030131-5299])

HSP 1 Score: 59.3066 bits (142), Expect = 5.845e-10
Identity = 34/103 (33.01%), Postives = 54/103 (52.43%), Query Frame = -1 
Query:  802 PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSL 1110
            PDR    ++ ++ A +F  R +RCY L ++ V  +F + + AR  +R   +  +  RI     EH L  P + +  L   ++ +N+ ML  L I EPRSF SL
Sbjct:   15 PDRYWKVQELLKNARHFRGRKSRCYSLAVRAVRRAFVYASKARKAKRRSMRQLWITRIAAATREHGLKYPAL-VHQLLKCSVLLNRRMLCDLSITEPRSFLSL 116
[Close]
BLAST of SMED30005154 vs. Ensembl Xenopus
Match: marchf7 (membrane associated ring-CH-type finger 7 [Source:Xenbase;Acc:XB-GENE-962645])

HSP 1 Score: 60.077 bits (144), Expect = 4.675e-10
Identity = 35/124 (28.23%), Postives = 66/124 (53.23%), Query Frame = -1 
Query:  784 LSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1155
            + FL L+R V  +  PDR    ++ ++ A +F  R NRC+ L ++ V  +F +   AR  ++   +  +  RI     EH +  P + + +L    + +N+ ++  + IYEP++FKSL  + K+
Sbjct:    1 MVFLSLSRWVRSR-GPDRYWRVQEVLKHARHFRGRKNRCFSLAVRAVRRAFVYSTKARKAKKRIMRALWVSRIAGATREHGMKYP-MLMCNLVKCQVALNRKVISDMAIYEPKTFKSLAALAKR 122
[Close]
BLAST of SMED30005154 vs. Ensembl Mouse
Match: Mrpl20 (mitochondrial ribosomal protein L20 [Source:MGI Symbol;Acc:MGI:2137221])

HSP 1 Score: 65.4698 bits (158), Expect = 4.728e-12
Identity = 40/122 (32.79%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  784 LLLTRRVIMKPK-PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1146
            + LT R+ ++ +  DR    ++ ++ A +F  R NRCY+L ++ V  +F     AR L++   +  +  RI     EH L  P  FI +L    +E+N+ +L  L IYEP++FKSL  + K+
Sbjct:    2 VFLTTRLWLRNRLTDRYWRVQEVLKHAQHFRGRKNRCYRLAVRAVTRAFVKCTKARRLKKRNLRTLWINRITAASQEHGLKYPA-FIVNLIKCQVELNRKVLVDLAIYEPKTFKSLAALAKR 122
[Close]
BLAST of SMED30005154 vs. Ensembl Mouse
Match: Mrpl20 (mitochondrial ribosomal protein L20 [Source:MGI Symbol;Acc:MGI:2137221])

HSP 1 Score: 65.4698 bits (158), Expect = 4.728e-12
Identity = 40/122 (32.79%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  784 LLLTRRVIMKPK-PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1146
            + LT R+ ++ +  DR    ++ ++ A +F  R NRCY+L ++ V  +F     AR L++   +  +  RI     EH L  P  FI +L    +E+N+ +L  L IYEP++FKSL  + K+
Sbjct:    2 VFLTTRLWLRNRLTDRYWRVQEVLKHAQHFRGRKNRCYRLAVRAVTRAFVKCTKARRLKKRNLRTLWINRITAASQEHGLKYPA-FIVNLIKCQVELNRKVLVDLAIYEPKTFKSLAALAKR 122
[Close]
BLAST of SMED30005154 vs. UniProt/SwissProt
Match: sp|Q9CQL4|RM20_MOUSE (39S ribosomal protein L20, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl20 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.307e-11
Identity = 40/122 (32.79%), Postives = 67/122 (54.92%), Query Frame = -1 
Query:  784 LLLTRRVIMKPK-PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1146
            + LT R+ ++ +  DR    ++ ++ A +F  R NRCY+L ++ V  +F     AR L++   +  +  RI     EH L  P  FI +L    +E+N+ +L  L IYEP++FKSL  + K+
Sbjct:    2 VFLTTRLWLRNRLTDRYWRVQEVLKHAQHFRGRKNRCYRLAVRAVTRAFVKCTKARRLKKRNLRTLWINRITAASQEHGLKYPA-FIVNLIKCQVELNRKVLVDLAIYEPKTFKSLAALAKR 122
[Close]
BLAST of SMED30005154 vs. UniProt/SwissProt
Match: sp|Q9BYC9|RM20_HUMAN (39S ribosomal protein L20, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL20 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.504e-11
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = -1 
Query:  802 DRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSL 1107
            DR    ++ ++ A +F  R NRCY+L ++ VI +F     AR+L++   +  +  RI     EH L  P + I +L    +E+N+ +L  L IYEP++FKSL
Sbjct:   16 DRYFRIQEVLKHARHFRGRKNRCYRLAVRTVIRAFVKCTKARYLKKKNMRTLWINRITAASQEHGLKYPAL-IGNLVKCQVELNRKVLADLAIYEPKTFKSL 116
[Close]
BLAST of SMED30005154 vs. UniProt/SwissProt
Match: sp|Q2TBR2|RM20_BOVIN (39S ribosomal protein L20, mitochondrial OS=Bos taurus OX=9913 GN=MRPL20 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 4.088e-11
Identity = 43/126 (34.13%), Postives = 66/126 (52.38%), Query Frame = -1 
Query:  784 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1161
            +FLS  L  R  I     DR    ++ ++ A +F  R NRCY+L ++ V  +F     AR L++   +  +  RI     EH L  P  FI +L    +E+N+ +L  L IYEP++FKSL  + K+
Sbjct:    2 VFLSAPLWLRNRIT----DRYWRVQEVLKHARHFRGRKNRCYRLAVRAVTRAFVRCTRARSLKKRHLRTLWINRITAASQEHGLKYPA-FILNLIKCQVELNRKVLADLAIYEPKTFKSLAALSKR 122
[Close]
BLAST of SMED30005154 vs. UniProt/SwissProt
Match: sp|Q5U4Z8|RM20_XENLA (39S ribosomal protein L20, mitochondrial OS=Xenopus laevis OX=8355 GN=mrpl20 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.125e-9
Identity = 35/124 (28.23%), Postives = 66/124 (53.23%), Query Frame = -1 
Query:  784 LSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQ 1155
            + FL L+R V  +  PDR    ++ ++ A +F  R NRC+ L ++ V  +F +   AR  ++   +  +  RI     EH +  P + + +L    + +N+ ++  + IYEP++FKSL  + K+
Sbjct:    1 MVFLSLSRWVRSR-GPDRYWRVQEVLKHARHFRGRKNRCFSLAVRAVRRAFVYSTKARKAKKRIMRALWVSRIAGATREHGMKYP-MLMSNLVKCQVALNRKVISDMSIYEPKTFKSLAALAKR 122
[Close]
BLAST of SMED30005154 vs. UniProt/SwissProt
Match: sp|B0K3L3|RL20_THEPX (50S ribosomal protein L20 OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=rplT PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.756e-7
Identity = 28/105 (26.67%), Postives = 56/105 (53.33%), Query Frame = -1 
Query:  778 REKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYL 1092
            R+K ++ A  +    ++ +++  + V+ S  +    R LR+  ++  +  RI      H + S   FI  L+   IEIN+ ML  + I++ ++F+ LV++ KQ+L
Sbjct:   14 RKKILKLAKGYWGAKSKLFRVANQAVMKSLMYAYIGRKLRKRDFRRLWITRINAAARAHGI-SYSRFINGLKKAGIEINRKMLSEMAIHDEKAFEELVNIAKQHL 117
[Close]
BLAST of SMED30005154 vs. TrEMBL
Match: A0A5J6BUC9 (Adhesive organ protein 1 OS=Minona ileanae OX=60013 PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 5.553e-53
Identity = 103/312 (33.01%), Postives = 169/312 (54.17%), Query Frame = -3 
Query: 1322 KDWLKWFKAFKLVKVEMLSGRTLGIHKNIPSSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKG-----NFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMTKLDYRDTM 2242
            +D+  + K   L+  E+ + R  G     P SFD  ++G++     S      ++GP++   + G GL LKG         P  + + LP  +   +VCG++I  +N+    ++ +  + W++D  + +D++ +P  G   +  ++      R + FDS+PA K+ +M     G     ++ F+ +C Y NT  + DC EK+D N RF  LP   PISIKCP++CI    CWG+KVYS+DS IC++A   FGR+ DG YT+YKI   + F  + +P LNH  +CR S +++  FTF+R+ YD    +Y   +
Sbjct:   10 RDYDAFVKMQTLINNEIKNNRAYG---ETPISFDFFIHGIQAKATSSKEYLSKSYGPSEISPLTGRGLLLKGVKDGKPKGQPQLIKLMLP--RTPVDVCGVLIGGVNNTEKFYVDSFEIHWRSDLGMKFDMLTDPKTGNSIFNTKLSKKNEFRAIFFDSVPA-KYFIMKLFYKGDRPGIRITFIHSCYYPNTKFLSDCGEKLDHNSRFDFLPVGTPISIKCPIDCILYRQCWGYKVYSSDSAICYSAVHAFGRYADGLYTLYKIANVSRFIYQPDPSLNHKAYCRGSSVQHSGFTFERRLYDEANKEYESHL 315
[Close]
BLAST of SMED30005154 vs. TrEMBL
Match: A0A5P8I4P2 (Trig-1 protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.452e-52
Identity = 107/291 (36.77%), Postives = 159/291 (54.64%), Query Frame = -3 
Query: 1346 FKLVKVEMLSGRTLGIHKNIP-SSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMT 2215
            +++V  E+   R +G+ K++  +  D     LK+  +P  +    +   N     + NG+  K +   PI L V LP  + + NVCGL+I    + G + I  + ++W+  S+ PW+L+A PT G+  + L +  N   +V+I D +PA +  L+L     K  + +L+ + NC+Y  T+ I DC E  ++N + QN   E P+S+ CP  C  S  C GFKVYST SPICWAA Q FG  GDG Y +Y I     F SE++P LN+G+FCR S       TF R YYD T
Sbjct:   25 WQIVSEELKEKRAIGLPKDLTLTGADFYEENLKFQTNPKSVSSSADSIQNDGKFNSENGIMFKPS-ETPIVLDVVLP--RNKSNVCGLLIRPGKN-GKDVIKTIEIIWKGSSKGPWNLVAVPTTGDLGFNLTISANNENKVLIIDPVPAEELKLILHADKEKGSSIRLSVMHNCQYSPTMKISDCLESFETNEKLQNSQTEGPVSVSCPTTCNSSSVCVGFKVYSTASPICWAAHQVFGSNGDGNYFLYVIPRVPFFKSEVDPTLNNGIFCRSSNQASPGITFSRVYYDYT 311
[Close]
BLAST of SMED30005154 vs. TrEMBL
Match: G4V5L6 (Putative 50s ribosomal protein L20 OS=Schistosoma mansoni OX=6183 GN=Smp_004980 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.412e-35
Identity = 73/162 (45.06%), Postives = 98/162 (60.49%), Query Frame = -1 
Query:  676 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYY 1161
            M L+  L  R+++     DR   ++  M+FAF F  R  RC+KLG  +VI +FQ+M +AR +RRN  K  + ER+E    +  L S     E L   NI +NKN+LQIL IYEPR+F +LVD+ KQY +E     PN+     VITRG+L SP+VPGNR  Y
Sbjct:    1 MKLTDALCIRKIVPFMPCDRWFRKDNLMKFAFFFKGRMARCHKLGTNRVIKAFQYMKDAREVRRNEMKCLWMERLEIASEQCGLPSARALCEGLAQSNIALNKNILQILSIYEPRTFSALVDLSKQYHLEKGVSVPNMSSPQTVITRGLLTSPVVPGNRNLY 162
[Close]
BLAST of SMED30005154 vs. TrEMBL
Match: A0A183M3Z5 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS10771 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 2.344e-35
Identity = 73/162 (45.06%), Postives = 99/162 (61.11%), Query Frame = -1 
Query:  676 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYY 1161
            M L+ +L  R+++     DR   R+  M+FAF F  R  RC+KLG  +VI +FQ+M + R +RR   K+ + ER+E    +  L S     E L   NI +NKNMLQIL IYEPR+F +LVD+ KQY +E     PN+    +VIT+G+L SPIVPGNR  Y
Sbjct:    1 MKLTDVLSIRKIVPFMPCDRWFRRDNLMKFAFFFKGRTARCHKLGTNRVIRAFQYMKDVREVRRKEMKSLWMERLEIASEQCGLPSARALCEGLAQSNIALNKNMLQILSIYEPRTFSALVDLSKQYHLEKGVSVPNMSSPQRVITQGLLTSPIVPGNRNLY 162
[Close]
BLAST of SMED30005154 vs. TrEMBL
Match: A0A3Q0KBS3 (Putative 50s ribosomal protein L20 OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 5.369e-35
Identity = 69/144 (47.92%), Postives = 90/144 (62.50%), Query Frame = -1 
Query:  676 DRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYY 1107
            DR   ++  M+FAF F  R  RC+KLG  +VI +FQ+M +AR +RRN  K  + ER+E    +  L S     E L   NI +NKN+LQIL IYEPR+F +LVD+ KQY +E     PN+     VITRG+L SP+VPGNR  Y
Sbjct:    4 DRWFRKDNLMKFAFFFKGRMARCHKLGTNRVIKAFQYMKDAREVRRNEMKCLWMERLEIASEQCGLPSARALCEGLAQSNIALNKNILQILSIYEPRTFSALVDLSKQYHLEKGVSVPNMSSPQTVITRGLLTSPVVPGNRNLY 147
[Close]
BLAST of SMED30005154 vs. Ensembl Cavefish
Match: mrpl20 (mitochondrial ribosomal protein L20 [Source:ZFIN;Acc:ZDB-GENE-030131-5299])

HSP 1 Score: 60.4622 bits (145), Expect = 1.608e-10
Identity = 32/96 (33.33%), Postives = 53/96 (55.21%), Query Frame = -1 
Query:  787 THRH-----NRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVK 1059
            THRH     NRCYKL ++ V  +F +   AR ++R   +  +  RI     EH L  P + I +L   ++++N+ +L  L I EPR+F++L  + +
Sbjct:   19 THRHFRGRKNRCYKLAVRTVCRAFVYATKARKVKRRSMRTLWISRIAAASREHGLKYPAL-IHNLVKSSVQLNRRILSDLAITEPRTFQALATLAR 113
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BLAST of SMED30005154 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011017.1 (pep scaffold:Pmarinus_7.0:GL498625:2086:2463:-1 gene:ENSPMAG00000009990.1 transcript:ENSPMAT00000011017.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.4394 bits (106), Expect = 4.518e-6
Identity = 30/102 (29.41%), Postives = 52/102 (50.98%), Query Frame = -1 
Query:  790 AREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVV 1095
            +R K  R A  FT R N C+ L +++V  S Q+    R +++   +  +   I     EH + S   F   L   NI++++ +L  L + EP SFK++++ V
Sbjct:    2 SRRKVFRLAKGFTGRSNNCFGLALRRVHKSLQYAYRDRKVKKRRTRRAWIHTINAAVREHGI-SYSRFQFGLTRSNIQLDRKILSDLAVNEPFSFKAVIEEV 102
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BLAST of SMED30005154 vs. Ensembl Nematostella
Match: EDO38461 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCU5])

HSP 1 Score: 64.6994 bits (156), Expect = 1.486e-12
Identity = 32/111 (28.83%), Postives = 58/111 (52.25%), Query Frame = -1 
Query:  778 PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYL 1110
             DR V R +    A  F  R   CY + I++V  + Q+   +R L++   ++ +  RI     EH++ +    +  +  ++I++N+ ML  + I+EPRSFK L  + K+ L
Sbjct:    7 ADRAVKRARIFALAKGFRGRSKNCYSIAIRRVQKALQYQYTSRRLKKRDMRSLWITRINIAGREHNI-NYSTLVHKMAKLDIQLNRKMLSEIAIHEPRSFKGLASLAKRRL 116
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BLAST of SMED30005154 vs. Ensembl Medaka
Match: mrpl20 (mitochondrial ribosomal protein L20 [Source:NCBI gene;Acc:101171059])

HSP 1 Score: 64.3142 bits (155), Expect = 1.005e-11
Identity = 32/108 (29.63%), Postives = 58/108 (53.70%), Query Frame = -1 
Query:  787 PDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVK 1110
            PDR    +  ++ A +F  R NRCY L ++ V  +F +   +R L++   +  +  RI     EH +  P + + +L   ++++N+ ++  L I EPR+F+SL  V K
Sbjct:   15 PDRFWKVQDLLKHARHFRGRKNRCYSLAVRAVRRAFVYATKSRKLKKRNMRTLWITRIAAATREHGMTYPAL-MHNLTKSSVQLNRRVISDLAITEPRTFQSLAKVAK 121
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BLAST of SMED30005154 vs. Planmine SMEST
Match: SMESG000049720.1 (SMESG000049720.1)

HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = -3 
Query: 1316 MSRYSLSVFFILLFAVQFSRVEPHKDWLKWFKAFKLVKVEMLSGRTLGIHKNIPSSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMTKLDYRDTMGA 2314
            MSRYSLSVFFILLFAVQFSRVEPHKDWLKWFKAFKLVKVEMLSGRTLGIHKNIPSSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMTKLDYRDTMGA
Sbjct:    1 MSRYSLSVFFILLFAVQFSRVEPHKDWLKWFKAFKLVKVEMLSGRTLGIHKNIPSSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMTKLDYRDTMGA 333
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BLAST of SMED30005154 vs. Planmine SMEST
Match: SMESG000049718.1 (SMESG000049718.1)

HSP 1 Score: 334.724 bits (857), Expect = 1.811e-110
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = -1 
Query:  673 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF 1161
            MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF
Sbjct:    1 MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF 163
[Close]
BLAST of SMED30005154 vs. Planmine SMEST
Match: SMESG000049718.1 (SMESG000049718.1)

HSP 1 Score: 308.916 bits (790), Expect = 9.395e-101
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = -1 
Query:  673 MKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF 1122
            MKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF
Sbjct:    1 MKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVISSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIEINKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGMLNSPIVPGNRKYYF 150
[Close]
BLAST of SMED30005154 vs. Planmine SMEST
Match: SMESG000049582.1 (SMESG000049582.1)

HSP 1 Score: 196.438 bits (498), Expect = 3.336e-56
Identity = 107/291 (36.77%), Postives = 159/291 (54.64%), Query Frame = -3 
Query: 1346 FKLVKVEMLSGRTLGIHKNIP-SSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLNPITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDLIANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKLAFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPCWGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHGVFCRKSILEYQAFTFDRKYYDMT 2215
            +++V  E+   R +G+ K++  +  D     LK+  +P  +    +   N     + NG+  K +   PI L V LP  + + NVCGL+I    + G + I  + ++W+  S+ PW+L+A PT G+  + L +  N   +V+I D +PA +  L+L     K  + +L+ + NC+Y  T+ I DC E  ++N + QN   E P+S+ CP  C  S  C GFKVYST SPICWAA Q FG  GDG Y +Y I     F SE++P LN+G+FCR S       TF R YYD T
Sbjct:   25 WQIVSEELKEKRAIGLPKDLTLTGADFYEENLKFQTNPKSVSSSADSIQNDGKFNSENGIMFKPS-ETPIVLDVVLP--RNKSNVCGLLIRPGKN-GKDVIKTIEIIWKGSSKGPWNLVAVPTTGDLGFNLTLSANNENKVLIIDPVPAEELKLILHADKEKGSSIRLSVMHNCQYSPTMKISDCLESFETNEKLQNSQTEGPVSVSCPTTCNSSSVCVGFKVYSTASPICWAAHQVFGSNGDGNYFLYVIPRVPFFKSEVDPTLNNGIFCRSSNQASPGITFSRVYYDYT 311
The following BLAST results are available for this feature:
BLAST of SMED30005154 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL207.296e-1235.29mitochondrial ribosomal protein L20 [Source:HGNC S... [more]
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BLAST of SMED30005154 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl-203.550e-832.84Mitochondrial Ribosomal Protein, Large [Source:Un... [more]
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BLAST of SMED30005154 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mRpL202.064e-1028.57gene:FBgn0036335 transcript:FBtr0075897[more]
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BLAST of SMED30005154 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl205.845e-1033.01mitochondrial ribosomal protein L20 [Source:ZFIN;A... [more]
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BLAST of SMED30005154 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
marchf74.675e-1028.23membrane associated ring-CH-type finger 7 [Source:... [more]
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BLAST of SMED30005154 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrpl204.728e-1232.79mitochondrial ribosomal protein L20 [Source:MGI Sy... [more]
Mrpl204.728e-1232.79mitochondrial ribosomal protein L20 [Source:MGI Sy... [more]
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BLAST of SMED30005154 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9CQL4|RM20_MOUSE3.307e-1132.7939S ribosomal protein L20, mitochondrial OS=Mus mu... [more]
sp|Q9BYC9|RM20_HUMAN3.504e-1135.2939S ribosomal protein L20, mitochondrial OS=Homo s... [more]
sp|Q2TBR2|RM20_BOVIN4.088e-1134.1339S ribosomal protein L20, mitochondrial OS=Bos ta... [more]
sp|Q5U4Z8|RM20_XENLA1.125e-928.2339S ribosomal protein L20, mitochondrial OS=Xenopu... [more]
sp|B0K3L3|RL20_THEPX2.756e-726.6750S ribosomal protein L20 OS=Thermoanaerobacter sp... [more]
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BLAST of SMED30005154 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J6BUC95.553e-5333.01Adhesive organ protein 1 OS=Minona ileanae OX=6001... [more]
A0A5P8I4P21.452e-5236.77Trig-1 protein OS=Schmidtea mediterranea OX=79327 ... [more]
G4V5L61.412e-3545.06Putative 50s ribosomal protein L20 OS=Schistosoma ... [more]
A0A183M3Z52.344e-3545.06Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A3Q0KBS35.369e-3547.92Putative 50s ribosomal protein L20 OS=Schistosoma ... [more]
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BLAST of SMED30005154 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl201.608e-1033.33mitochondrial ribosomal protein L20 [Source:ZFIN;A... [more]
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BLAST of SMED30005154 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000011017.14.518e-629.41pep scaffold:Pmarinus_7.0:GL498625:2086:2463:-1 ge... [more]
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BLAST of SMED30005154 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005154 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO384611.486e-1228.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30005154 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl201.005e-1129.63mitochondrial ribosomal protein L20 [Source:NCBI g... [more]
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BLAST of SMED30005154 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000049720.10.000e+0100.00SMESG000049720.1[more]
SMESG000049718.11.811e-110100.00SMESG000049718.1[more]
SMESG000049718.19.395e-101100.00SMESG000049718.1[more]
SMESG000049582.13.336e-5636.77SMESG000049582.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005154 ID=SMED30005154|Name=SMED30005154|organism=Schmidtea mediterranea sexual|type=transcript|length=2469bp
GCATTACACCCATTACATGTAAATAGATAAACAAGATCCTAACACAATTC
AACTTATAATTGAATATATTGTATACGTAAAGAATTTGTAATCTAATTTT
CTTAAGCGCTTATTTGTCTTCTTATAAGTTGTATAACCTCTCGAATAAGG
AAATATCACCACAGATAAACGCTTAATCAAAGTTTTCACAAATACACTAT
CGATCATTATGATTATATCTACTCAAATAAAATTTGAACCCATAACTGGA
AAGCCTACAGACTTTGAATATTAAAATAAAGAACGTAATTAACCATTTAA
ATGATTAACTTATTTTCTCTTTGATATTAATACAAAACTTTGACAAGCGT
TATTTAAATGTGACCTAAAATGTAGCATAAATTATTCAGCGCATTGCTGG
TTCATAATATGATCTTAACACCCGAAATATTTAAAAACCTTAAAATTCAA
GTGTTCACAAAATAATCAAAAAATAAAAGTAACAAAAATCAACCAATAAA
ATCACTCGGTTAATAAAAAATAAAATTTAACGATTACTATACAAACACGA
AATTATAAATACAAATATTACACAATTTCAATGCAAATTTTTTCAATATA
AATTCATAAAATTAAAATAAAGGCAACAAAAGCCAAATTTTGCTAATTAT
CCACCGAATTGAATTTATTCTAAAAATAATATTTTCTATTTCCGGGAACA
ATCGGACTATTTAACATTCCCCGAGTAATGACCTTTGCTCCAGGTCCTAT
GTTTGGAAATTTATAATCATTTTCAATTAAATATTGCTTCACAACATCTA
CTAAACTTTTAAAGCTTCTTGGTTCATAGATGCATAAAATTTGCAGCATA
TTTTTATTGATTTCTATGTTCATATGTCTCAATGATTCAATAAAAATTTC
GGGAGATCCTAAATCATGTTCTAAACATCCACGTTCTATTCGTTCAGCGA
AATAATTTTTATAAAAATTCCTTCGCAAATGACGAGCATTATTCATAAAT
TGAAATGAGGAGATAACTTTTTTAATGCCCAACTTGTAACAACGATTATG
CCTATGTGTAAAATTAAATGCAAATCTCATAAACTTTTCTCTGGCAACTA
TGCGATCAGGTTTAGGTTTCATTATCACTCTTCTTGTTAATAGTAAAAAA
CTCAAAAACATCTTATATTTATTTTAAAAGAACCACACAAAAATAATATT
TAAAACAAAATGCATTTAATTTTCAATTACATTTAAATAAATTTATTAAT
GAAATCATGTTTTAATTATCCACAACCAGTTAAATATTTGAAACGTTAAA
TTTTTAACGCTTTCATGCGCCCATGGTATCTCGATAATCTAGTTTCGTCA
TATCATAATATTTTCTGTCAAATGTAAATGCTTGATACTCTAGAATTGAC
TTTCTACAAAACACTCCGTGATTAAGCAGTGGATTTAGTTCTGAATTAAA
TGCAGCTGCTGCCTGAATTTTATAGACTGTATAATATCCATCTCCCCACC
GTCCAAAGTTTTGAGTTGCTGCCCAACAGATAGGAGAATCCGTAGAGTAG
ACCTTAAATCCCCAACACGGCCAACTTCGAATGCAGTTTACAGGGCATTT
TATGCTTATTGGAACTTCAAATGGCAAATTCTGGAAGCGAGGATTTGAAT
CAATCTTTTCGGAGCAATCTACGATATTAATCGTGTTTTTGTAGCGACAA
TTTCTTAGAAACGCCAACTTAAAATTGACATTTTTTCCTGGAATGGTAGT
CAACATCAGCATAAATTTTAAGGCCGGAATCGAATCAAAAATCACCACCC
TGGTATTCAAATTCGGTCTCATCCGAAGATAATATTCATATTCTCCGTTT
GTGGGATTTGCAATAAGGTCCCACGGTATTCGAGAATCCGCTTGCCACAT
TAATCTCATGTTGAAAATGAATTCATTCCCAGCATCATTTAGGGAAGTTA
TTACAAGACCGCAAACGTTTTGCTGTTTCTTTAGCACAGGAAGGCCAACA
GTTAAAGTTATCGGATTGAGAAAATTTCCTTTCAACGTTAAACCATTTCC
ATTAATAAGGTCAGCTTTATTCGGACCATGGTTAACAGGATCTACCAATA
TGGAAGGATCTATTCCGTATTTCAACCCATTCATTACGATATCAAACGAT
GATGGTATGTTTTTATGTATTCCTAAAGTTCGTCCACTCAACATTTCAAC
TTTAACCAATTTGAAAGCCTTGAACCATTTCAACCAATCTTTATGTGGCT
CAACTCGGGAAAATTGAACCGCGAAAAGAAGGATGAAAAAAACGCTTAGA
CTGTACCGGCTCATTCCAAAATTTAGTAAGTTAGGGAACTTAATAATATA
ATTGTCACATATTTCCAGTAGGCTTTTGTATTTGTCAGATGAGTTTCTCA
AGCCGTTACTTACAATCGATAGCAGCACATGTGTTTATGGGCAAATGTTT
CACTTCACTGATCTCGAAC
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protein sequence of SMED30005154-orf-1

>SMED30005154-orf-1 ID=SMED30005154-orf-1|Name=SMED30005154-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp
MSRYSLSVFFILLFAVQFSRVEPHKDWLKWFKAFKLVKVEMLSGRTLGIH
KNIPSSFDIVMNGLKYGIDPSILVDPVNHGPNKADLINGNGLTLKGNFLN
PITLTVGLPVLKKQQNVCGLVITSLNDAGNEFIFNMRLMWQADSRIPWDL
IANPTNGEYEYYLRMRPNLNTRVVIFDSIPALKFMLMLTTIPGKNVNFKL
AFLRNCRYKNTINIVDCSEKIDSNPRFQNLPFEVPISIKCPVNCIRSWPC
WGFKVYSTDSPICWAATQNFGRWGDGYYTVYKIQAAAAFNSELNPLLNHG
VFCRKSILEYQAFTFDRKYYDMTKLDYRDTMGA*
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protein sequence of SMED30005154-orf-2

>SMED30005154-orf-2 ID=SMED30005154-orf-2|Name=SMED30005154-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MFLSFLLLTRRVIMKPKPDRIVAREKFMRFAFNFTHRHNRCYKLGIKKVI
SSFQFMNNARHLRRNFYKNYFAERIERGCLEHDLGSPEIFIESLRHMNIE
INKNMLQILCIYEPRSFKSLVDVVKQYLIENDYKFPNIGPGAKVITRGML
NSPIVPGNRKYYF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000421tail
PLANA:0000429neoblast
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036609LCCL_sf
IPR004043LCCL
IPR005813Ribosomal_L20
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
GO:0019843rRNA binding
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004043LCCL domainPFAMPF03815LCCLcoord: 216..267
e-value: 3.5E-5
score: 23.8
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.75
IPR036609LCCL domain superfamilySUPERFAMILYSSF69848LCCL domaincoord: 234..268