SMED30005146

Overview
NameSMED30005146
Smed IDSMED30005146
Length (bp)2579
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30005146 (SMED30005146) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30005146 (SMED30005146) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30005146 (SMED30005146) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30005146 (SMED30005146) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30005146

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neuronSMED30005146h1SMcG0006308 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30005146h1SMcG0006308 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30005146h1SMcG0006308 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30005146h1SMcG0014995 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30005146h1SMcG0014995 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30005146h1SMcG0014995 dd_Smed_v6_1671_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30005146 vs. TrEMBL
Match: A0A1S3HAE6 (uncharacterized protein LOC106153553 OS=Lingula unguis OX=7574 GN=LOC106153553 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 5.738e-13
Identity = 89/356 (25.00%), Postives = 147/356 (41.29%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEW-RFRDSD-KEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERG-SLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFY 2379
            F S+  +V LSI+ GND T  Y + L + L  ++   K   V + A ++   K     I   P + R L  NPQ   ++    +FYTL              D       L+++  + +    +   I + H   ++   ++E      P     + ++R+ IY  VL    + V E+ R  DSD K   +   HA          P   N + D     + LF     H E    L+FF+++  + GF+  ILRYF  L   +NL ++ VEF ++  T F   G           K   +  + I P  +C+ + +WF  +Y     F G    L ++FP   ++ + +   AFY
Sbjct:  249 FRSHRLLVDLSIVNGNDFTAHYMKSL-YNLLDVRGAKK---VENFAGWMRHYK----GIENHPILARELQYNPQFATAVMTSRQFYTLTAPP----------DRPTMKCPLSEYLEQAVQHGALPSNIMAMHSNFYWHRFLLEDSTPGLPCAEVALQSLRTFIYRMVLPRYENYVYEYGRTPDSDLKTIQVHAAHASL--------PPVSNIKPDRTFESLKLFHRIMMHQEPDFELNFFQRYGRKSGFIFCILRYFLVLNWGQNLYITPVEFLSVLATAF---GWEHH-------KDQWYQNIAIRPSSRCVTIGNWFQALYRYAYNFLGQMLYLNQEFPPPHEIFAGARWTAFY 568          
BLAST of SMED30005146 vs. TrEMBL
Match: A0A210QAR5 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT01511 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 6.917e-12
Identity = 80/358 (22.35%), Postives = 149/358 (41.62%), Query Frame = 1
Query: 1330 YDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAK-----PESENERDDSIIYGVDLFDETWSHLER--GS-LHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFY 2379
            +D +V LSI+ GND T  Y  K     K+   + K   +  VA++I ++   P+              +P   + ++    F    ++  N+ N  +       +  L+    K + +     ++   H   ++   ++E  +E  P     ++ +R+ +Y  VL      V+E+           P E  P +T  + A      P     + D I + +  F    SH+ER  GS   +F+++  + GF  Y+LRYF  L   +NL V++ EF AL        G     K++         G+ + P  +C+ + SW+ ++Y+    F G   +++ +FP   +  S +V   FY
Sbjct:  250 HDLLVELSIVGGNDFTGQYIHKGGLQQKL---EIKGRGIEGVADWI-RDHTVPET-------------HPVFAQEMRHNMGFRGAVVHSRNFYNLGVPPLKPSKTGHLSQILEKGIREGHFVARVMGMHNNFYWHRAVVEDLSEGQPCAEVALAPLRAHVYRIVLQRHESMVEEYGRS--------PWE--PMRTAGVLAVEDSNIPSIHRIQPDKIFWNLKHFHYIMSHMERRTGSESKWFERYGRKTGFSVYLLRYFLLLNWGRNLFVTENEFLALLAM---SLGRPSAAKYQ---------GICVRPTPRCVTLGSWYQDLYYHAYCFLGKLLSISHEFPLPCEAYSGAVWTVFY 568          
BLAST of SMED30005146 vs. TrEMBL
Match: A0A0L8GFX0 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22034150mg PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.233e-11
Identity = 80/359 (22.28%), Postives = 157/359 (43.73%), Query Frame = 1
Query: 1327 SYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAK--PESENERDDSIIYGVDLFDETWSHLERG------SLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFY 2379
            S D+++ L+I+ GND T  Y +  Q  L  +  + +   + ++  ++   K   ++    P +D  +  N +   +++    FY L++ ++  +N+K          SLT+   ++L    +  K+  AH G ++  V+ E      P   +L+S +RS IY  +L      V ++ +   D+   I     P    + K K  P+ E  + ++I + + +F +  +  E G        ++F ++    GF+ Y LRYF  L    NL +++ EF  L   +F         K E + +R       + P  +C+ + +W  +I+     + G    LT++FP    + S S    +Y
Sbjct:  248 SSDELIELAIVCGNDLTSHYMRNNQTLLYKLDIRERKNQLENIVAWVKSYKRIENH----PLLDNEMRNNSEFALAVQLSRSFYKLELVQDYDDNNK---------GSLTELVIERLKTKTLHPKMLGAHHGLYWQKVLFEDLYNYWPGIETLLSNLRSCIYRILLPLNEMGVIDYGW---DQSLTI----MPHTVSVAKWKWLPQFEEIKKENISHNLKIFHKIITQQEPGWKTQDWPRNYFIRYGRHNGFIFYCLRYFLLLNWQHNLFITEREFLTLICLLFTH-------KEEKQYQR-----YPLTPTSRCITINNWMQDIFHHAYGYLGTLLFLTEEFPRPATLFSGSTWVVYY 574          
BLAST of SMED30005146 vs. TrEMBL
Match: K1QJE9 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10014990 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.602e-11
Identity = 89/409 (21.76%), Postives = 166/409 (40.59%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDK---EFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSH---LERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ*THKLCI 2529
            F S   +V LSII GND T  Y          I+ +     V   A ++ + +   ++    P ++R ++ N     +++    FY L+   E            V     +    +K+S  +    + + H   ++  ++ E      P     ++ +R+ IY  VL    + V E+     D    E ++ ++            P     ++D I + ++ F    SH   +ER    +F ++  + GF+ Y LRYF  L   +NL++ Q EF AL   +F             + + + +  ++I P  +C+ + +WF+++Y     F G    LT +FP  E++ S SV   FY    +D          T   + +   + VL+   DQ +AV +   H + +
Sbjct:  249 FQSPHLMVELSIIAGNDFTSQYVSTGLNRQIDIRGRIG---VETFAEWVKRYRGIENH----PMLNREMSRNVSFARAVEHSRMFYCLQSNPEEI----------VHKGYFSKLLAEKISSLKYPSHLMAMHNNFYWHRLLQEDTTRGQPCAEEALTELRALIYRIVLPRRENLVNEYGRSPWDTLRIEGVLAIDDPNL--------PALHKIQEDKIFWNLNSFHYIMSHQEPVERPK--WFDRYGRKNGFIVYCLRYFLLLNWGRNLQLQQQEFLALCALVF------------ARPREEHYQQIQIRPTPRCVSIGNWFLDVYRHAFLFLGKLLYLTHEFPLPEEIYSGSVWTCFYMCCKDD----------TYYAASRQTTQEVLSWIQDQMNAVISDKRHPIML 608          
BLAST of SMED30005146 vs. TrEMBL
Match: A0A2T7PUB9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04002 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.297e-9
Identity = 58/210 (27.62%), Postives = 97/210 (46.19%), Query Frame = 1
Query: 1765 CPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSL---HFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSAS---VLYAF 2376
            C     L++ +R+ +Y  +L      V E+  +   ++F     +A    +L    P   + R++ I   + LF    + LE G      +F K+  + GF+ YILRYF  L   + L V++ EF AL  T F   G S+E  ++          +++CP  +CL + + F +IY     F G   +L  +FP  +++ S S   VLYA 
Sbjct:  381 CAPAEELLAPLRADLYAILLPRHKAFVTEYG-QTMRQDFQQMQNYAVNDKNL----PSLNHIRENQIFRNMRLFHYIMTRLEPGQAPEKTWFDKYGRKTGFIFYILRYFLLLNWGQCLNVTEAEFLALVATAF---GASKESSWQE---------LRVCPDPRCLTIAALFQDIYRHAFLFLGSVLHLKHEFPLPKEIFSGSMWTVLYAL 573          
BLAST of SMED30005146 vs. Planmine SMEST
Match: SMESG000001486.1 (SMESG000001486.1)

HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ 2508
            FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRKKLSKSEISKKIYSAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ
Sbjct:  243 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRKKLSKSEISKKIYSAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ 638          
BLAST of SMED30005146 vs. Planmine SMEST
Match: SMESG000001486.1 (SMESG000001486.1)

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ 2508
            FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRKKLSKSEISKKIYSAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ
Sbjct:  251 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRKKLSKSEISKKIYSAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVTTQ 646          
BLAST of SMED30005146 vs. Planmine SMEST
Match: SMESG000032650.1 (SMESG000032650.1)

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 1
Query:   40 MPALKGFIIALEDNCSINRINHLEMTSLLISLTVCYLXXXXXXXXXXMLATMMLICMYRINTRATFYTIYFWILYIQLFKLFINVTFYEMQYILIFGSGMTALFMAFVIYSYNIFFYIYMSVDAIFFYLLAGLSVLNLIRLQKPFQIINKHIIHLSCSVIVFLAFLFNMIILYNTQAFICTNSGKIGFIYTTQLWKLPILLCLVFFHIIIPWVTSIFTTIYVYKLSRRLGSKSSNLKRXXXXXXXXXXXXXXPTITTLVSWILHRDIFLYQSLAMIMSDIGQYTSVLFILYILFVTDAKFRKLFLRFNPRLTESVEXXXXXXXXXXXXXXXXXXXXXXXNDEVVTNKVXXXXXXXXXXXXXXXXRPSKMEIKSSEVSKERA 1182
            MPALKGFIIALEDNCSINRINHLEMTSLLISLTVCYLIIIIIANIVIMLATMMLICMYRINTRATFYTIYFWILYIQLFKLFINVTFYEMQYILIFGSGMTALFMAFVIYSYNIFFYIYMSVDAIFFYLLAGLSVLNLIRLQKPFQIINKHIIHLSCSVIVFLAFLFNMIILYNTQAFICTNSGKIGFIYTTQLWKLPILLCLVFFHIIIPWVTSIFTTIYVYKLSRRLGSKSSNLKRLALFINLIFILLAAPTITTLVSWILHRDIFLYQSLAMIMSDIGQYTSVLFILYILFVTDAKFRKLFLRFNPRLTESVESKYYTSNSRYKSTKTSYSTETWTNDEVVTNKVLKPPKSFSKSFISLLSRPSKMEIKSSEVSKERA
Sbjct:    1 MPALKGFIIALEDNCSINRINHLEMTSLLISLTVCYLIIIIIANIVIMLATMMLICMYRINTRATFYTIYFWILYIQLFKLFINVTFYEMQYILIFGSGMTALFMAFVIYSYNIFFYIYMSVDAIFFYLLAGLSVLNLIRLQKPFQIINKHIIHLSCSVIVFLAFLFNMIILYNTQAFICTNSGKIGFIYTTQLWKLPILLCLVFFHIIIPWVTSIFTTIYVYKLSRRLGSKSSNLKRLALFINLIFILLAAPTITTLVSWILHRDIFLYQSLAMIMSDIGQYTSVLFILYILFVTDAKFRKLFLRFNPRLTESVESKYYTSNSRYKSTKTSYSTETWTNDEVVTNKVLKPPKSFSKSFISLLSRPSKMEIKSSEVSKERA 381          
BLAST of SMED30005146 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 368.622 bits (945), Expect = 7.703e-117
Identity = 190/394 (48.22%), Postives = 271/394 (68.78%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVT 2502
            F+++++I+ LSI+KGNDNT +Y++ L + L I ++  +  H++ VA YI  N V  +N+F L GM  FLNENP+ + ++ FC++FYTL+ ++       I FDP  A  SLT F ++KLSK EIS  +Y +H G +  V  +EKP +   + +++MS IRS+IYPYVL  +L  V E++ RD++   +I V H+PFK++LI +KP+ E+ER + + YG+D+F+E WSHLE  SL+F  +F LE GFLCYILRY YK+T   N++++  EF A+F  +FY+Y +S  + F T L +DIFLGMKICP EKCL ++SWFM IY   KYF GGA NL KDFP AE VMS++V  AFYE  +ND N+RV+HLDE+V+  I+DL   +L      Y A+T
Sbjct:  218 FSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEG-ILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLE-NSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAIT 609          
BLAST of SMED30005146 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 368.237 bits (944), Expect = 1.008e-116
Identity = 190/395 (48.10%), Postives = 272/395 (68.86%), Query Frame = 1
Query: 1321 FTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTHVANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDFFRXXXXXXXXXXXXXXAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIYPYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDSIIYGVDLFDETWSHLE-RGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLKVSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYSWFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRVEHLDETVVESIKDLGESVLAHFSDQYDAVT 2502
            F+++++I+ LSI+KGNDNT +Y++ L + L I ++  +  H++ VA YI  N V  +N+F L GM  FLNENP+ + ++ FC++FYTL+ ++       I FDP  A  SLT F ++KLSK EIS  +Y +H G +  V  +EKP +   + +++MS IRS+IYPYVL  +L  V E++ RD++   +I V H+PFK++LI +KP+ E+ER + + YG+D+F+E WSHLE + SL+F  +F LE GFLCYILRY YK+T   N++++  EF A+F  +FY+Y +S  + F T L +DIFLGMKICP EKCL ++SWFM IY   KYF GGA NL KDFP AE VMS++V  AFYE  +ND N+RV+HLDE+V+  I+DL   +L      Y A+T
Sbjct:  218 FSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLDRWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPIDAFATITTVMSEIRSRIYPYVLHADLDHVTEFKRRDAEG-ILINVVHSPFKSNLIISKPDPESERQNGVKYGIDIFEEIWSHLESQDSLNFVYQFGLEKGFLCYILRYLYKVTHGLNIEMTAKEFDAIFAMVFYRYRLSINMPFRTNLNQDIFLGMKICPTEKCLTIFSWFMEIYSTAKYFCGGALNLLKDFPKAENVMSSTVFIAFYENNVNDINKRVDHLDESVIGMIQDLENEILECLDGPYQAIT 611          
The following BLAST results are available for this feature:
BLAST of SMED30005146 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3HAE65.738e-1325.00uncharacterized protein LOC106153553 OS=Lingula un... [more]
A0A210QAR56.917e-1222.35Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
A0A0L8GFX01.233e-1122.28Uncharacterized protein OS=Octopus bimaculoides OX... [more]
K1QJE93.602e-1121.76Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A2T7PUB93.297e-927.62Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of SMED30005146 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30005146 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000001486.10.000e+0100.00SMESG000001486.1[more]
SMESG000001486.10.000e+0100.00SMESG000001486.1[more]
SMESG000032650.10.000e+0100.00SMESG000032650.1[more]
SMESG000033479.17.703e-11748.22SMESG000033479.1[more]
SMESG000033479.11.008e-11648.10SMESG000033479.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005146 ID=SMED30005146|Name=SMED30005146|organism=Schmidtea mediterranea sexual|type=transcript|length=2579bp
CTGTCATATTGATCAGTATTGAGCCAAGAATTATAGTCAATGCCAGCCTT
GAAAGGTTTCATCATAGCCCTAGAAGACAACTGTAGCATAAACCGGATCA
ATCACTTGGAGATGACGTCATTGCTGATCTCTCTGACGGTGTGCTACTTA
ATCATCATCATAATAGCCAACATAGTCATCATGCTAGCAACAATGATGCT
GATATGCATGTATCGGATAAATACACGGGCGACATTCTATACAATATATT
TCTGGATCTTATACATTCAGCTGTTCAAGCTCTTCATCAACGTGACATTC
TATGAAATGCAATACATTCTCATATTCGGGTCTGGAATGACAGCCTTGTT
CATGGCGTTTGTCATTTATTCATATAACATTTTCTTCTATATCTACATGT
CTGTCGATGCGATATTCTTCTATTTGCTGGCAGGGCTGTCGGTACTAAAT
CTGATAAGGCTGCAGAAGCCGTTCCAGATCATCAACAAACATATTATACA
CTTGTCATGCTCCGTCATCGTATTTCTCGCGTTTTTATTCAATATGATCA
TTCTCTACAATACTCAGGCATTCATTTGCACAAATTCTGGTAAAATTGGT
TTCATTTATACCACTCAATTATGGAAACTTCCCATTTTATTGTGCCTCGT
ATTCTTCCACATCATTATTCCGTGGGTGACATCAATCTTCACCACCATAT
ACGTATACAAGCTCAGTAGGAGACTTGGAAGTAAATCATCGAATTTGAAG
CGACTGGCTTTGTTTATAAATTTAATTTTCATCTTATTAGCAGCCCCTAC
CATTACCACCTTGGTGAGTTGGATCCTACATAGGGATATTTTCCTTTATC
AAAGTCTTGCGATGATTATGTCAGACATTGGGCAATATACGTCAGTGTTG
TTTATTCTCTACATACTCTTTGTCACAGACGCCAAGTTTAGGAAGCTGTT
TCTGAGATTCAATCCTAGATTGACAGAGTCTGTCGAATCGAAGTATTATA
CATCAAATTCCAGGTACAAGTCCACCAAGACCTCTTATTCAACAGAAACC
TGGACAAACGATGAAGTAGTAACCAACAAAGTATTGAAACCACCAAAATC
GTTTTCAAAATCCTTTATTTCTCTGCTTTCCAGACCATCGAAAATGGAAA
TAAAAAGTTCTGAAGTATCAAAAGAGAGAGCTTGAATGGATAAAATCAAA
GATAATGCTCAACAACAGCAACAATAACGAGAATGACGATGATAATGATG
ATGACGATCATACATATAAATACACACACACACATATATATATATTTGAA
ATGTTATAATTATCACCTAGTTTACGAGTTATGACCAAATTGTTGCACTG
AGCATTATCAAGGGCAATGACAATACTGAGGATTACGCTCAGAAACTTCA
GTTTTATCTGAAAATCATCAAAAATCAAACCAAACATGCACACGTGACTC
ATGTTGCCAATTATATTGTAAAAAATAAGGTGACTCCTGATAATATTTTC
AGACTTCCTGGAATGGATAGATTTCTAAACGAAAATCCTCAGTGTAGGGA
ATCGATAAAATTCTGCTATGAATTTTATACCTTGAAAATTTACAAGGAAA
ATTATGAAAATGATAAAATTATTTTTGATCCAAACGTTGCAAGTGATTCT
TTGACAGATTTCTTTCGAAAAAAATTATCAAAATCTGAAATTTCCAAGAA
AATATATTCAGCCCATAAAGGAACTTTCTATGATGTTGTTATTATGGAAA
AACCCAATGAAGAATGCCCTTCTAATTCGTCTTTAATGAGCACTATTCGT
TCAAAGATTTATCCATATGTTCTGGATACCGAACTTCATGAAGTAAAAGA
ATGGAGATTTCGAGATTCGGATAAAGAGTTTATAATTCCAGTGGAACATG
CCCCGTTTAAAACTGATCTAATAAAAGCCAAACCCGAAAGTGAAAACGAG
CGTGATGACTCCATTATTTATGGGGTTGATTTATTTGATGAAACTTGGTC
TCATTTGGAACGAGGATCATTACATTTTTTCAAAAAGTTTGATTTGGAAA
TAGGATTTCTTTGCTATATATTGAGATATTTCTATAAATTGACTAAAAAT
AAAAATTTAAAGGTGTCACAAGTAGAATTTAAAGCTTTGTTTATTACTAT
ATTCTATCAATACGGAGTTTCTCAAGAAATCAAGTTTGAAACAAAGTTAA
AAAGAGATATATTTTTGGGAATGAAAATTTGCCCAATGGAAAAATGTTTA
ATTATGTATTCGTGGTTTATGAATATTTATTTTATGGGAAAATATTTCAC
CGGAGGTGCTTTTAATTTGACTAAAGATTTCCCTACAGCGGAAAAAGTGA
TGAGTGCAAGCGTGCTATACGCATTCTACGAATATTATATGAATGATAGG
AATAGAAGAGTAGAACATTTGGATGAAACGGTTGTGGAATCAATCAAGGA
TCTTGGGGAATCGGTTTTAGCGCATTTTTCCGATCAATACGATGCTGTAA
CTACACAGTGAACGCATAAGCTTTGCATTTGTGCATATTTATAAAAACAT
TTAATTTATTTATTGATTTTAGACTTCAC
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protein sequence of SMED30005146-orf-1

>SMED30005146-orf-1 ID=SMED30005146-orf-1|Name=SMED30005146-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=333bp
MDRFLNENPQCRESIKFCYEFYTLKIYKENYENDKIIFDPNVASDSLTDF
FRKKLSKSEISKKIYSAHKGTFYDVVIMEKPNEECPSNSSLMSTIRSKIY
PYVLDTELHEVKEWRFRDSDKEFIIPVEHAPFKTDLIKAKPESENERDDS
IIYGVDLFDETWSHLERGSLHFFKKFDLEIGFLCYILRYFYKLTKNKNLK
VSQVEFKALFITIFYQYGVSQEIKFETKLKRDIFLGMKICPMEKCLIMYS
WFMNIYFMGKYFTGGAFNLTKDFPTAEKVMSASVLYAFYEYYMNDRNRRV
EHLDETVVESIKDLGESVLAHFSDQYDAVTTQ*
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protein sequence of SMED30005146-orf-2

>SMED30005146-orf-2 ID=SMED30005146-orf-2|Name=SMED30005146-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=382bp
MPALKGFIIALEDNCSINRINHLEMTSLLISLTVCYLIIIIIANIVIMLA
TMMLICMYRINTRATFYTIYFWILYIQLFKLFINVTFYEMQYILIFGSGM
TALFMAFVIYSYNIFFYIYMSVDAIFFYLLAGLSVLNLIRLQKPFQIINK
HIIHLSCSVIVFLAFLFNMIILYNTQAFICTNSGKIGFIYTTQLWKLPIL
LCLVFFHIIIPWVTSIFTTIYVYKLSRRLGSKSSNLKRLALFINLIFILL
AAPTITTLVSWILHRDIFLYQSLAMIMSDIGQYTSVLFILYILFVTDAKF
RKLFLRFNPRLTESVESKYYTSNSRYKSTKTSYSTETWTNDEVVTNKVLK
PPKSFSKSFISLLSRPSKMEIKSSEVSKERA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0000463cholinergic neuron
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 65..87
NoneNo IPR availableTMHMMTMhelixcoord: 114..136
NoneNo IPR availableTMHMMTMhelixcoord: 273..295
NoneNo IPR availableTMHMMTMhelixcoord: 92..109
NoneNo IPR availableTMHMMTMhelixcoord: 241..263
NoneNo IPR availableTMHMMTMhelixcoord: 28..50
NoneNo IPR availableTMHMMTMhelixcoord: 199..221
NoneNo IPR availableTMHMMTMhelixcoord: 157..179