UPF0546 membrane protein AGAP012180

Overview
NameUPF0546 membrane protein AGAP012180
Smed IDSMED30005099
Length (bp)1658
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of UPF0546 membrane protein AGAP012180 (SMED30005099) t-SNE clustered cells

Violin plots show distribution of expression levels for UPF0546 membrane protein AGAP012180 (SMED30005099) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of UPF0546 membrane protein AGAP012180 (SMED30005099) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for UPF0546 membrane protein AGAP012180 (SMED30005099) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30005099

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30005099SMESG000012908.1 Contig3122newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005099SMESG000012908.1 Contig3122uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005099SMESG000064518.1 Contig3122newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30005099SMESG000064518.1 Contig3122uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Human
Match: TMEM234 (transmembrane protein 234 [Source:HGNC Symbol;Acc:HGNC:28837])

HSP 1 Score: 47.3654 bits (111), Expect = 8.627e-6
Identity = 33/111 (29.73%), Postives = 54/111 (48.65%), Query Frame = 1
Query: 1132 WGLTNVLMKL----FQTDDSPIYK------------NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            WG T  L+K      Q    P +             N ++LM FLLNQ  S+ + +TL  + +++AVPI NSL  + + I G AL  +     +  G+   ++G+ +C T+
Sbjct:   18 WGGTQPLLKRASAGLQRVHEPTWAQQLLQEMKTLFLNTEYLMPFLLNQCGSLLYYLTLASTDLTLAVPICNSLAIIFTLIVGKALGEDIGGKRAVAGMVLTVIGISLCITS 128          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Fly
Match: CG12929 (gene:FBgn0033429 transcript:FBtr0088541)

HSP 1 Score: 57.7658 bits (138), Expect = 9.485e-10
Identity = 39/114 (34.21%), Postives = 58/114 (50.88%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKL-----------------FQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICC 1410
            VG++WG+TN  ++L                 F  +   I    ++ + F LNQ  S  ++ TL+ + I+VAVP+ NSL+F  +AI GYAL  EKL      G   +L  L +CC
Sbjct:   11 VGLLWGVTNPFIRLGSQGIESVGDTGSKWRNFVQEARTIGSRWRYWIPFGLNQCGSALYVWTLQRASITVAVPVANSLSFAFTAITGYAL-GEKLP-----GRKVILGTLLVCC 118          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Fly
Match: CG12929 (gene:FBgn0033429 transcript:FBtr0113060)

HSP 1 Score: 51.9878 bits (123), Expect = 5.469e-8
Identity = 34/95 (35.79%), Postives = 51/95 (53.68%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKL-----------------FQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKL 1353
            VG++WG+TN  ++L                 F  +   I    ++ + F LNQ  S  ++ TL+ + I+VAVP+ NSL+F  +AI GYAL  EKL
Sbjct:   11 VGLLWGVTNPFIRLGSQGIESVGDTGSKWRNFVQEARTIGSRWRYWIPFGLNQCGSALYVWTLQRASITVAVPVANSLSFAFTAITGYAL-GEKL 104          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Zebrafish
Match: tmem234 (transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GENE-090313-326])

HSP 1 Score: 53.5286 bits (127), Expect = 3.501e-8
Identity = 36/112 (32.14%), Postives = 56/112 (50.00%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKLFQTDDSPIYK----------------NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            V I+WG TN  +K        + K                N K+L+ FLLNQS S+ F  TL  + +S+AVP+ NSLTF+ + + G  L  E     + +G+  ++ G+ +C
Sbjct:   13 VAILWGGTNPFLKKGTEGIEEVQKKNKLLQFLAELKFLFLNFKYLVPFLLNQSGSLVFYFTLASTDLSLAVPVVNSLTFLFTLLMGKVLGEEFGGKGAIMGMLLIMCGVTVC 124          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Zebrafish
Match: tmem234 (transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GENE-090313-326])

HSP 1 Score: 53.5286 bits (127), Expect = 3.967e-8
Identity = 36/112 (32.14%), Postives = 56/112 (50.00%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKLFQTDDSPIYK----------------NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            V I+WG TN  +K        + K                N K+L+ FLLNQS S+ F  TL  + +S+AVP+ NSLTF+ + + G  L  E     + +G+  ++ G+ +C
Sbjct:   13 VAILWGGTNPFLKKGTEGIEEVQKKNKLLQFLAELKFLFLNFKYLVPFLLNQSGSLVFYFTLASTDLSLAVPVVNSLTFLFTLLMGKVLGEEFGGKGAIMGMLLIMCGVTVC 124          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Zebrafish
Match: tmem234 (transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GENE-090313-326])

HSP 1 Score: 53.5286 bits (127), Expect = 3.967e-8
Identity = 36/112 (32.14%), Postives = 56/112 (50.00%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKLFQTDDSPIYK----------------NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            V I+WG TN  +K        + K                N K+L+ FLLNQS S+ F  TL  + +S+AVP+ NSLTF+ + + G  L  E     + +G+  ++ G+ +C
Sbjct:   13 VAILWGGTNPFLKKGTEGIEEVQKKNKLLQFLAELKFLFLNFKYLVPFLLNQSGSLVFYFTLASTDLSLAVPVVNSLTFLFTLLMGKVLGEEFGGKGAIMGMLLIMCGVTVC 124          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Mouse
Match: Tmem234 (transmembrane protein 234 [Source:MGI Symbol;Acc:MGI:1924049])

HSP 1 Score: 47.3654 bits (111), Expect = 4.935e-6
Identity = 27/78 (34.62%), Postives = 46/78 (58.97%), Query Frame = 1
Query: 1183 IYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            ++ N ++LM FLLNQS S+ + +TL  + +++AVPI NSL  V + I G  L  +     +  G+   + G+ +C T+
Sbjct:   51 LFGNTEYLMPFLLNQSGSLLYYLTLASTDLTLAVPICNSLAIVFTLIVGKVLGEDIGGKEAVAGMVLTITGITVCITS 128          
BLAST of UPF0546 membrane protein AGAP012180 vs. UniProt/SwissProt
Match: sp|A0NGI1|TM234_ANOGA (Transmembrane protein 234 homolog OS=Anopheles gambiae OX=7165 GN=AGAP012180 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.944e-9
Identity = 39/130 (30.00%), Postives = 65/130 (50.00%), Query Frame = 1
Query: 1066 SNTLNVKMDLVIILIQCFVGIVWGLTNVLMKLFQ------TDDSPIYK----------NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
             NT+   +D+  +L    V I+WG TN  +K           DS + +            ++L+  ++NQ  SI +++TL+ + +S+ VP+ NSLTFV +AI    L   +  W  + G+  V+LG  IC
Sbjct:    5 ENTVPASVDIYAVLSILLVAIMWGATNPFIKRGSIGYNELKADSKLGQLWLEVRFLITRWQYLLPLVINQLGSIVYVLTLQRTELSLTVPMANSLTFVFTAITARLLGERQSGWKIYCGMTLVILGTVIC 134          
BLAST of UPF0546 membrane protein AGAP012180 vs. UniProt/SwissProt
Match: sp|Q54ZG7|TM234_DICDI (Transmembrane protein 234 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0277575 PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 2.853e-8
Identity = 41/126 (32.54%), Postives = 64/126 (50.79%), Query Frame = 1
Query: 1087 MDLVIILIQCFVGIVWGLTNVLMKLFQTDDSPIYKN----------------LKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            MD   I+    VG +WG TN L+K      S + K+                  + +  L+N S S+ F  TL    IS+ VPI+NSLTF+ +++ G  L  + L + S++G+ FVL G+ IC ++
Sbjct:    1 MDTYNIISLLLVGFIWGGTNPLIKRGSEGVSKVKKDNFLSQIIYEFVYLWTRPSYTIPMLINLSGSVVFFYTLSKVDISLVVPISNSLTFLFTSLMGMLLGEKVLHFKSYLGMIFVLAGVTICVSS 126          
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Match: A0A267DPK2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001788g2 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.338e-13
Identity = 39/115 (33.91%), Postives = 73/115 (63.48%), Query Frame = 1
Query: 1099 IILIQCFVGIVWGLTNVLMKL---FQTDDSPIY------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            ++L+   VG+VWG+TN LMK      T DS  +      +N K+L +   NQS S+ F+ +L  + +S+AVP+TN++TF+++++ G+ +N EKL+  + + +  +  G+++C T+
Sbjct:    1 MLLLCAAVGLVWGVTNALMKASTAADTSDSKAWSLGALLRNWKYLTALAANQSASLLFLWSLAEASLSIAVPLTNTVTFLATSLTGWLINGEKLAPTTLLAMALMSAGIYLCVTS 115          
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Match: E2BHZ2 (UPF0546 membrane protein AGAP012180 OS=Harpegnathos saltator OX=610380 GN=EAI_05688 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.437e-13
Identity = 50/137 (36.50%), Postives = 78/137 (56.93%), Query Frame = 1
Query: 1048 SKFYFVSNTLNVKMDLVIILIQCFVGIVWGLTNVLMK-----LFQTDDSPIY-----------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICC 1410
            S+  F+  T +  +D VI L  C V I+WG+TN  +K     L       +Y            NLK+++ FLLNQ  S+ + +TL+ + IS+AVP++NSLTFV +AI G+ L  EK+   +++G+  +L G  +CC
Sbjct:    7 SRLIFIDVTFDFFIDSVIYL--CLVAILWGVTNPFIKKGAQGLEDVKAMSVYGQFIQEILFLVTNLKYILPFLLNQCGSVLYYLTLQSTDISLAVPVSNSLTFVFTAITGWFLGEEKVHRNTYMGMILILCGTILCC 141          
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Match: A0A151XJ06 (Uncharacterized protein OS=Trachymyrmex zeteki OX=64791 GN=ALC60_00800 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.590e-13
Identity = 48/123 (39.02%), Postives = 73/123 (59.35%), Query Frame = 1
Query: 1090 DLVIILIQCFVGIVWGLTNVLMK-----LFQTDDSPIY-----------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICC 1410
            D VI L  CFV I+WG+TN  +K     L   +   +Y            NLK+++ FLLNQ  S+ + +TL+ S +S+AVP++NSLTFV +A+ G+ L  EK+   +++G+  VL G  +CC
Sbjct:    7 DTVIYL--CFVAILWGVTNPFIKKGAQGLENINAKSVYGQFFKEQLFLITNLKYIVPFLLNQCGSVLYFLTLQSSDLSLAVPVSNSLTFVFTAVTGWFLGEEKVHRNTYLGMILVLCGTMLCC 127          
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Match: A0A158NEG4 (Uncharacterized protein OS=Atta cephalotes OX=12957 GN=105619002 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.328e-13
Identity = 49/124 (39.52%), Postives = 73/124 (58.87%), Query Frame = 1
Query: 1087 MDLVIILIQCFVGIVWGLTNVLMK-----LFQTDDSPIY-----------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICC 1410
            +D VI L  CFV I+WG+TN  +K     L       +Y            NLK+++ FLLNQ  S+ + +TL+ S +S+AVP++NSLTFV +AI G+ L  EK+   +++G+  VL G  +CC
Sbjct:    5 IDSVIYL--CFVAILWGVTNPFIKKGAQGLEDIRAKSVYGQFFKEQLFLITNLKYIVPFLLNQCGSVLYFLTLQNSDLSLAVPVSNSLTFVFTAITGWFLGEEKVHRNTYLGMILVLCGTMLCC 126          
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Match: A0A195FLA5 (Uncharacterized protein (Fragment) OS=Trachymyrmex septentrionalis OX=34720 GN=ALC56_04277 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 2.900e-13
Identity = 49/124 (39.52%), Postives = 73/124 (58.87%), Query Frame = 1
Query: 1087 MDLVIILIQCFVGIVWGLTNVLMK-----LFQTDDSPIY-----------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICC 1410
            +D VI L  CFV I+WG+TN  +K     L       +Y            NLK+++ FLLNQ  S+ + +TL+ S +S+AVP++NSLTFV +AI G+ L  EK+   +++G+  VL G  +CC
Sbjct:    3 VDSVIYL--CFVAILWGVTNPFIKKGAQGLEAIRAKSVYGQFFKEQLFLITNLKYIVPFLLNQCGSVLYFLTLQSSDLSLAVPVSNSLTFVFTAITGWFLGEEKVHRNTYLGMILVLCGTMLCC 124          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Cavefish
Match: tmem234 (transmembrane protein 234 [Source:NCBI gene;Acc:103036571])

HSP 1 Score: 54.6842 bits (130), Expect = 1.451e-8
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKL----------------FQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            V  +WG TN L+K                 F  +   ++ NLK+L+ FLLNQS S+ F +TL  + +S+AVP  NSL+F+ + + G  L  +     + +G+   +LG+ +C
Sbjct:   13 VAALWGGTNPLLKRATVGIEHVKEGSRISQFLAEVKFLFLNLKYLLPFLLNQSGSVVFYITLATADLSLAVPTVNSLSFLFTMLTGKLLGEDFGGKRAVLGMVLTMLGVTLC 124          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Sea Lamprey
Match: tmem234 (transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GENE-090313-326])

HSP 1 Score: 48.9062 bits (115), Expect = 2.149e-7
Identity = 34/112 (30.36%), Postives = 56/112 (50.00%), Query Frame = 1
Query: 1120 VGIVWGLTNVLMKLFQTDDSPIY----------------KNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            V ++WG TN L+K        +                  N ++++ F+LNQS S+ +  TL  + +SVAVP+TNSLTF+ + I G  L        + +G+  + +GL +C
Sbjct:   15 VALLWGGTNPLLKRGAKGVETVRGRGRLRQLFAELCFLVSNPRYMVPFVLNQSGSLLYYFTLGSADLSVAVPLTNSLTFIFTLITGKLLGENIGGTRALLGMLLMGVGLTLC 126          
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Nematostella
Match: EDO37684 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF34])

HSP 1 Score: 59.3066 bits (142), Expect = 1.003e-10
Identity = 31/72 (43.06%), Postives = 48/72 (66.67%), Query Frame = 1
Query: 1192 NLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFIC 1407
            N K+++ FLLNQS S+ F +TL  + +S+AVPITNSLTF+ + + G  L  E     ++IG+  V+ G+ +C
Sbjct:   55 NWKYMVPFLLNQSGSVLFYLTLGSADLSLAVPITNSLTFIMTTLTGRILGEESCKTETYIGMLLVVGGVSLC 126          
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Match: SMESG000012908.1 (SMESG000012908.1)

HSP 1 Score: 214.542 bits (545), Expect = 1.198e-67
Identity = 109/110 (99.09%), Postives = 109/110 (99.09%), Query Frame = 1
Query: 1087 MDLVIILIQCFVGIVWGLTNVLMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            MDLVIILIQCFVGIVWGLTN LMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA
Sbjct:    1 MDLVIILIQCFVGIVWGLTNALMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 110          
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Match: SMESG000012908.1 (SMESG000012908.1)

HSP 1 Score: 174.096 bits (440), Expect = 1.404e-52
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 1
Query: 1153 MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA
Sbjct:    1 MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 88          
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Match: SMESG000012908.1 (SMESG000012908.1)

HSP 1 Score: 171.785 bits (434), Expect = 1.333e-51
Identity = 91/110 (82.73%), Postives = 91/110 (82.73%), Query Frame = 1
Query: 1087 MDLVIILIQCFVGIVWGLTNVLMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            MDLVIILIQCFVGIVWGLTN LMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKF                 S IAGYALNNEKLSWISFIGIGFVLLGLFICCTA
Sbjct:    1 MDLVIILIQCFVGIVWGLTNALMKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKF-----------------STIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 93          
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Match: SMESG000012908.1 (SMESG000012908.1)

HSP 1 Score: 131.339 bits (329), Expect = 9.208e-37
Identity = 70/88 (79.55%), Postives = 70/88 (79.55%), Query Frame = 1
Query: 1153 MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 1416
            MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKF                 S IAGYALNNEKLSWISFIGIGFVLLGLFICCTA
Sbjct:    1 MKLFQTDDSPIYKNLKFLMSFLLNQSMSIAFMVTLKF-----------------STIAGYALNNEKLSWISFIGIGFVLLGLFICCTA 71          
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Match: SMESG000026037.1 (SMESG000026037.1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.889e-14
Identity = 45/156 (28.85%), Postives = 84/156 (53.85%), Query Frame = 3
Query:  153 ISLTNFNDIETIYNSIRNMNRLSINMESCFYISLDEMIKYYLENYF--ILSKISINNSLEFCGFALYFTEYNRNIGWGVYVDDLFVAESYRRKGMCRLQMKNIAKLCKANNLKFIKLIYQNLNVHHFEIYKKFGFKN-----TGLQLLEIFGKENI 599
            I   N  DI T+++   N+ +    M   F +S + M++ +  N F  ++     + + +  G+ LY  EY+   G+G Y+DD +V+   R KG+ +L M +I  +   N ++F+KL++Q   +   ++Y  FGF N     +G+Q+ EIFG++ +
Sbjct:    4 ICKANAEDISTVFDFTNNLVQHHEGMTEFFLMSQESMLEAFNNNAFECLILFDDESETKKAVGYMLYMEEYSLLHGYGYYIDDFYVSPEIRGKGLGKLLMHHIFNIASENKVQFLKLVFQKSILGLEKMYISFGFDNISSPLSGVQMYEIFGRDEV 159          
The following BLAST results are available for this feature:
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TMEM2348.627e-629.73transmembrane protein 234 [Source:HGNC Symbol;Acc:... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG129299.485e-1034.21gene:FBgn0033429 transcript:FBtr0088541[more]
CG129295.469e-835.79gene:FBgn0033429 transcript:FBtr0113060[more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tmem2343.501e-832.14transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GEN... [more]
tmem2343.967e-832.14transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GEN... [more]
tmem2343.967e-832.14transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GEN... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tmem2344.935e-634.62transmembrane protein 234 [Source:MGI Symbol;Acc:M... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|A0NGI1|TM234_ANOGA1.944e-930.00Transmembrane protein 234 homolog OS=Anopheles gam... [more]
sp|Q54ZG7|TM234_DICDI2.853e-832.54Transmembrane protein 234 homolog OS=Dictyostelium... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DPK21.338e-1333.91Uncharacterized protein OS=Macrostomum lignano OX=... [more]
E2BHZ21.437e-1336.50UPF0546 membrane protein AGAP012180 OS=Harpegnatho... [more]
A0A151XJ061.590e-1339.02Uncharacterized protein OS=Trachymyrmex zeteki OX=... [more]
A0A158NEG42.328e-1339.52Uncharacterized protein OS=Atta cephalotes OX=1295... [more]
A0A195FLA52.900e-1339.52Uncharacterized protein (Fragment) OS=Trachymyrmex... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2341.451e-832.14transmembrane protein 234 [Source:NCBI gene;Acc:10... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tmem2342.149e-730.36transmembrane protein 234 [Source:ZFIN;Acc:ZDB-GEN... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO376841.003e-1043.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of UPF0546 membrane protein AGAP012180 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000012908.11.198e-6799.09SMESG000012908.1[more]
SMESG000012908.11.404e-52100.00SMESG000012908.1[more]
SMESG000012908.11.333e-5182.73SMESG000012908.1[more]
SMESG000012908.19.208e-3779.55SMESG000012908.1[more]
SMESG000026037.11.889e-1428.85SMESG000026037.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30005099 ID=SMED30005099|Name=UPF0546 membrane protein AGAP012180|organism=Schmidtea mediterranea sexual|type=transcript|length=1658bp
TTTTTTTTTAAAAAATTTAATTTTTAAATCATTTTTATTCCTTTTTTAAA
TATTTAAAATTGCTTTAAGAATATTTCTTAAAATTATTTCCATTATTAAC
AATTTCGGTGACTTAGGTTTATTTTATATCAATGTCTGAAACAAATTGTG
TAATAAGTCTTACAAACTTTAATGATATTGAAACTATTTATAATAGTATT
CGTAATATGAATCGGTTATCAATTAACATGGAAAGTTGTTTTTATATATC
GCTAGACGAAATGATAAAATATTACCTTGAGAACTATTTTATATTATCAA
AAATATCAATTAACAATTCATTAGAATTTTGTGGTTTTGCTCTATATTTT
ACTGAATACAATAGAAATATTGGATGGGGTGTGTATGTTGATGATTTGTT
TGTTGCTGAAAGCTACAGAAGAAAAGGAATGTGTCGTTTACAAATGAAAA
ATATTGCTAAATTATGCAAAGCAAATAACCTAAAATTCATTAAATTAATT
TATCAAAATTTAAATGTTCATCATTTTGAAATATATAAAAAGTTTGGATT
TAAAAATACTGGTTTGCAATTATTGGAAATTTTCGGTAAAGAAAATATCG
TAAAATTTAAAAATTTAGTTCTTGATGCAAACCTATGTCAAAACAATTCT
AAGATGAGTTTTGAAAAATCAAAGAAAGTAATTAATACTGGTGAAGTTTG
GTCAATTACAATACTTAATGATAATTCCTATCACTTTGATATACAAACAA
GCTATAATTGTTGGCTTGGTCCTATAATGTTTCTTAATAATTTTAGTGGT
AATTTGGTTGACATCGAAAGTAAAGATATTTGGATTGCATTAAACCATTT
TCTAAAAGAAAATATGGAAATTTTTGGTATGTGGGCTGATATAGATGATG
AGAATTTAGAAATTTTATTAAAGGAGATTGGTTTTACTAATATCACAAAA
GATGAATTTTGGACTTCTTTAATTCTCGATGAAAACGGAATCGAAAATTT
ATTGTAATAATATTTAATAAATTTAGCTTGGCTTTTTTTTAATTATATCA
AAATTTTATTTTGTGAGTAATACGTTAAATGTTAAGATGGATTTAGTAAT
TATTCTAATTCAATGTTTCGTCGGAATTGTTTGGGGACTAACAAATGTAC
TAATGAAATTATTTCAAACCGACGACTCTCCAATTTATAAAAATTTGAAG
TTCTTAATGTCATTTTTATTAAACCAGTCGATGTCAATTGCGTTTATGGT
CACTCTGAAATTTTCTATAATTTCTGTCGCTGTTCCTATTACGAATTCAT
TAACATTTGTATCTTCAGCAATTGCGGGTTACGCATTAAATAATGAGAAA
TTGAGTTGGATAAGTTTTATTGGAATTGGATTTGTGTTGCTGGGGCTTTT
TATTTGTTGTACTGCATAAAGAAATGTAAATAATAAATCTTTATAGTAAA
TTTCGTTATTTAAAATTGCAAAATCACGAAACAAAACAGCTGTAATGATT
GTAGAAGTGATGGACCCGTGGACAATCCAAATGCTCGGACACAGTTCCAG
CTTTGCTTAGCAACGGGTATCAAGCGGTAAGCAAAAATATTTGAATGGGG
TTGTCCGGTTAACAACCGCATATTCTCCAAAAAAATTTTAACACCCAGAA
ACCCACAG
back to top

protein sequence of SMED30005099-orf-1

>SMED30005099-orf-1 ID=SMED30005099-orf-1|Name=SMED30005099-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp
MDLVIILIQCFVGIVWGLTNVLMKLFQTDDSPIYKNLKFLMSFLLNQSMS
IAFMVTLKFSIISVAVPITNSLTFVSSAIAGYALNNEKLSWISFIGIGFV
LLGLFICCTA*
back to top

protein sequence of SMED30005099-orf-2

>SMED30005099-orf-2 ID=SMED30005099-orf-2|Name=SMED30005099-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=292bp
MSETNCVISLTNFNDIETIYNSIRNMNRLSINMESCFYISLDEMIKYYLE
NYFILSKISINNSLEFCGFALYFTEYNRNIGWGVYVDDLFVAESYRRKGM
CRLQMKNIAKLCKANNLKFIKLIYQNLNVHHFEIYKKFGFKNTGLQLLEI
FGKENIVKFKNLVLDANLCQNNSKMSFEKSKKVINTGEVWSITILNDNSY
HFDIQTSYNCWLGPIMFLNNFSGNLVDIESKDIWIALNHFLKENMEIFGM
WADIDDENLEILLKEIGFTNITKDEFWTSLILDENGIENLL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000101muscle cell
PLANA:0002056medial muscle cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR016181Acyl_CoA_acyltransferase
IPR000182GNAT_dom
IPR018908TMEM234
Vocabulary: molecular function
TermDefinition
GO:0008080N-acetyltransferase activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000182GNAT domainPFAMPF00583Acetyltransf_1coord: 65..140
e-value: 3.9E-7
score: 30.3
IPR000182GNAT domainPROSITEPS51186GNATcoord: 16..163
score: 11.245
NoneNo IPR availableGENE3DG3DSA:3.40.630.30coord: 7..161
e-value: 9.3E-16
score: 60.1
IPR016181Acyl-CoA N-acyltransferaseSUPERFAMILYSSF55729Acyl-CoA N-acyltransferases (Nat)coord: 8..149