4-coumarate--CoA ligase 2
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30005068 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Match: TEKT3 (tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293]) HSP 1 Score: 342.428 bits (877), Expect = 1.132e-103 Identity = 193/479 (40.29%), Postives = 290/479 (60.54%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQV-------KNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P + +I+ S R R+ +++ + S + P R S + +S SV P +NT + + + R +TR++P+DW SN +++ S R + +L+ D + +++ +TR QA+ T+ L ERV +I FW+ E+ E+ M E L + + L+ L T+ PL ++ CL RE R GID V DEVE L+ EV+TI CQE+M L K Q+ NRA+QHE EKDL DK ++++DDK +HL+NTS G+ Y G+E D T+S+P SWAKF+ DNI RSQ RA+S LR +I+ L+ AN MWN F+ VN S +R+ E +A+NK+++HL +T+QEIF E +I ++KAI DK LK+A+TRL+ERT+R N+ELC D A L E+ E+ ++I+ L+++ R AE L++ K+ LE D+AVK NSL IDQ+ C+ +RK +P
Sbjct: 7 TLTTTYAHPRP---TPTNFLPAISTMASSYRDRFPHSNLTHS-LSLPWRPSTYYKVASNSPSVAPYCTRSQRVSENTMLPFVSNRTTFFTRYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQADTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRIDDKCHHLRNTSDGVGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQLRLVNEVHEVDDTIQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Match: TEKT3 (tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293]) HSP 1 Score: 342.428 bits (877), Expect = 1.132e-103 Identity = 193/479 (40.29%), Postives = 290/479 (60.54%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQV-------KNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P + +I+ S R R+ +++ + S + P R S + +S SV P +NT + + + R +TR++P+DW SN +++ S R + +L+ D + +++ +TR QA+ T+ L ERV +I FW+ E+ E+ M E L + + L+ L T+ PL ++ CL RE R GID V DEVE L+ EV+TI CQE+M L K Q+ NRA+QHE EKDL DK ++++DDK +HL+NTS G+ Y G+E D T+S+P SWAKF+ DNI RSQ RA+S LR +I+ L+ AN MWN F+ VN S +R+ E +A+NK+++HL +T+QEIF E +I ++KAI DK LK+A+TRL+ERT+R N+ELC D A L E+ E+ ++I+ L+++ R AE L++ K+ LE D+AVK NSL IDQ+ C+ +RK +P
Sbjct: 7 TLTTTYAHPRP---TPTNFLPAISTMASSYRDRFPHSNLTHS-LSLPWRPSTYYKVASNSPSVAPYCTRSQRVSENTMLPFVSNRTTFFTRYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQADTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRIDDKCHHLRNTSDGVGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQLRLVNEVHEVDDTIQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Match: TEKT5 (tektin 5 [Source:HGNC Symbol;Acc:HGNC:26554]) HSP 1 Score: 306.22 bits (783), Expect = 5.193e-91 Identity = 162/402 (40.30%), Postives = 254/402 (63.18%), Query Frame = -2
Query: 3210 TARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4415
T R+ L++R+SP+DW+ SN + +EA R A+RL D+ + +++ + T Q + L +R+ +I FW+ EL E+ + E +NL+ R L+ PL ++ CL RE R GID V D VEK+LI+EV+ +K CQE+M + Q+ +IQM+ NR AQH E+DL+DKS + +D+K ++L+NTS +++ G+E D TIS+P +WAKFS DNI SQ RA+S LR E + L ++ MW F++ N + +R+ E+T+ +NKL++ L +T+QEIF E +I LL ++I KE PLK+A+TRLE RT+R N+ELC D L E+ + ++++ LK + R + L+ TK LE ++A+K N+L ID++ C+G+RK FP ++
Sbjct: 80 TLRSALFSRYSPHDWDQSNQLQVRGAEASRLWASRLTDDSMRLLQDKDQLTHQMQEGTCRNLGQRLSDIGFWKSELSYELDRLLTENQNLETVKRRLECAANEVNCPLQVALECLYHREKRIGIDLVHDNVEKNLIREVDLLKCCQEQMRKLAQRIDIQMRDNRDAQHVLERDLEDKSSAQCIDEKCFNLRNTSDCISFFHGMEKIDGTISVPETWAKFSNDNIKHSQNMRANSIQLREEAEHLFETLSDQMWRQFTDTNLAFNARISEVTDVKNKLQTQLAKTLQEIFQAENTIMLLERSIMAKEGPLKVAQTRLECRTRRPNMELCRDIPQLKLVNEVFTIDDTLQTLKLRLRETQDTLQLLVMTKCRLEHELAIKANTLCIDKEKCMGMRKTFPCTPRL 481
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Match: TEKT1 (tektin 1 [Source:HGNC Symbol;Acc:HGNC:15534]) HSP 1 Score: 228.024 bits (580), Expect = 1.020e-64 Identity = 137/391 (35.04%), Postives = 229/391 (58.57%), Query Frame = -2
Query: 3222 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHP-GIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPM 4391
+F P++W +N + ++A+R+++ RL +++ + V E E TR Q++ K+L +R++ + FW++ELD ++ + ++L + L++ LE ++PL I+E CL RE R GID V D VE LIKE E I+ + R L++ Q++MNR+A++ EKDLKDK + +DD + L N S + Y + + N++S+ W FS N+ ++ K R +S L+ +D ++ AN + V+ + + +++ +AR+KL HL + ++EI EK+I L KAI D+E P K+A TRLE RT R NVELC D A L KE+ E+ +++ LKE A+ L + L+++I VKEN++ ID+ C+ +RK P+
Sbjct: 10 KFLPSEWHIANKNQYHRADAQRSRSERLVAESQRLVDEIEKTTRKSQSDVNKKLEQRLEEVQFWKKELDDKLEQLVNVTDDLLIYKIRLEKALETLKEPLHITETCLAYREKRIGIDLVHDTVEHELIKEAEIIQGIMALLTRTLEEASEQIRMNRSAKYNLEKDLKDKFVALTIDDICFSLNNNSPNIRYSENAVRIEPNSVSLE-DWLDFSSTNVEKADKQRNNSLMLKALVDRILSQTANDLRKQCDVVDTAFKNGLKDTKDARDKLADHLAKVMEEIASQEKNITALEKAILDQEGPAKVAHTRLETRTHRPNVELCRDVAQYRLMKEVQEITHNVARLKETLAQAQAELKGLHRRQLALQEEIQVKENTIYIDEVLCMQMRKSIPL 399
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Match: TEKT4 (tektin 4 [Source:HGNC Symbol;Acc:HGNC:31012]) HSP 1 Score: 198.364 bits (503), Expect = 2.706e-54 Identity = 128/390 (32.82%), Postives = 216/390 (55.38%), Query Frame = -2
Query: 3225 TRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4394
+++ +W + A + + A+R Q+ R + ++ + ET+ + Q ++T+ + ER+Q+ W+ EL +E+ ++ E L + L+ L+ T+ P +I+ L RE R+ + V+D VE L+KE E I+ QE + R + + Q+++NR + CE D DK ++ +D+ + S+ + HP F + S P + AKF+QDN+ R+Q+ R +S LR +D ++R+ + + VN + R +E+ +AR KL HL +T++EI D E ++ L++AI DKE PL +A+TRL R+ R N+ELC D A L E+ EL S+ L+E+ AE L + LEKDIA NSL ID++ C+ R +P
Sbjct: 37 SKYLLEEWFQNCYARYHQAFADRDQSERQRHESQQLATETQALAQRTQQDSTRTVGERLQDTHSWKSELQREMEALAAETNLLLAQKQRLERALDATEVPFSITTDNLQCRERREHPNLVRDHVETELLKEAELIRNIQELLKRTIMQAVSQIRLNREHKETCEMDWSDKMEAYNIDETCGRHHSQSTEVQAHPYSTTFQESASTPETRAKFTQDNLCRAQRERLASANLRVLVDCILRDTSEDLRLQCDAVNLAFGRRCEELEDARYKLHHHLHKTLREITDQEHNVAALKQAIKDKEAPLHVAQTRLYLRSHRPNMELCRDAAQFRLLSEVEELNMSLTALREKLLEAEQSLRNLEDIHMSLEKDIAAMTNSLFIDRQKCMAHRTRYP 426
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Match: acs-6 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q23404]) HSP 1 Score: 114.39 bits (285), Expect = 1.028e-25 Identity = 105/349 (30.09%), Postives = 173/349 (49.57%), Query Frame = 1
Query: 247 TTAELIISYC----SAFPDKVALIDDARNIKYK-FSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNM--SPIGQLELNLDKD-LAILPYSSGTSGLPKGVMLTHSNFVS----SNKQLEM----THESKESDSY-----MLILPMYHVYGMICIPNFLFG---LGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGA-ASLDQQLENRLIEKFKVKNPDFFTTQ--GITDINKLIVI 1212
T E I C SA D V ID K K + DV+ +++++A+AL K G G +A N PE+L+ + A+ G ++ + +T E++ Q SN SI+F E+ L ++ +K V+ I + P + ++D + +P + +N+ D +A+LPYSSGT+G PKG LTH N + + LE KE ++ +L+LP YH YG+ N +F LG+ +V KFD + ML I+ YKV + L+PP+++FL P V F+ + K + A A+ +QL + ++F P+ + Q G+T++ + I
Sbjct: 16 TVHERIFEACRIHASANKDAVCFIDAETTTKKKLYKDVEPTVNSLATALVKLGFKPGDVASQAFPNCPEFLVAMLAVMKCGGAMSNASAIFTDYELQLQFCDSNTSIVFTDEDRLARIRRATAKCPGVRKIICL-RTFPLRTEFPENVLDYVELTQTPDQPINVNVSMDSIALLPYSSGTTGRPKGCQLTHRNIGAMLDVAKAHLETDVAPAMFGKEKATWHKEHTVLLLPWYHAYGL----NTMFETILLGMTGIVFKKFDTIVMLNRIKFYKVKLAWLVPPMLIFLAKDPMVPIFNTAPFLKVIMSAGATAGKQLCEEVSKRF----PNAWLCQAYGMTEMVQFTTI 355
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Match: acs-14 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q19339]) HSP 1 Score: 114.005 bits (284), Expect = 1.134e-25 Identity = 93/287 (32.40%), Postives = 146/287 (50.87%), Query Frame = 1
Query: 334 FSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVI----GNRIP-GYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSS-------NKQLEMTHESKESDSY----MLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKF 1146
F ++ A+A L G K +A ++ N Y + G V+ + +T E++ Q + S ++F E+ L KV + N++ I VI G+ +P G S +++ S ++ + Q+ +++ DL +LPYSSGT+G PKGVML+H NF S +K + D Y +L LP YHVYG + + L G+ +V F+P LTA++ YKV L L+PP+++FL HP D+FDLSSV+ + GAA + L L K+
Sbjct: 50 FEQLRKDAFAVAMYLHSIGFKK-DVAAVVLPNVWHYTSFFIGCAINGGAVSGASALFTDYELQRQFVDSRAKVVFTYEDFLPKVLLAVKQSPNIQKIIVIPKPVGSSLPAGVVSWNEVV--STPVTALPQVPIDVHNDLLVLPYSSGTTGPPKGVMLSHYNFTSMISMYLAIDKSHILDVLDPNWDCYNEKALLFLPFYHVYGFGLLNHCLLK-GMTGIVMSHFEPNNFLTAVQNYKVRCLCLVPPIMVFLAKHPICDKFDLSSVQMIMAGAAPAGKDLIEELKRKY 332
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Match: acs-14 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q19339]) HSP 1 Score: 114.005 bits (284), Expect = 1.134e-25 Identity = 93/287 (32.40%), Postives = 146/287 (50.87%), Query Frame = 1
Query: 334 FSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVI----GNRIP-GYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSS-------NKQLEMTHESKESDSY----MLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKF 1146
F ++ A+A L G K +A ++ N Y + G V+ + +T E++ Q + S ++F E+ L KV + N++ I VI G+ +P G S +++ S ++ + Q+ +++ DL +LPYSSGT+G PKGVML+H NF S +K + D Y +L LP YHVYG + + L G+ +V F+P LTA++ YKV L L+PP+++FL HP D+FDLSSV+ + GAA + L L K+
Sbjct: 50 FEQLRKDAFAVAMYLHSIGFKK-DVAAVVLPNVWHYTSFFIGCAINGGAVSGASALFTDYELQRQFVDSRAKVVFTYEDFLPKVLLAVKQSPNIQKIIVIPKPVGSSLPAGVVSWNEVV--STPVTALPQVPIDVHNDLLVLPYSSGTTGPPKGVMLSHYNFTSMISMYLAIDKSHILDVLDPNWDCYNEKALLFLPFYHVYGFGLLNHCLLK-GMTGIVMSHFEPNNFLTAVQNYKVRCLCLVPPIMVFLAKHPICDKFDLSSVQMIMAGAAPAGKDLIEELKRKY 332
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Match: acs-10 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:O02200]) HSP 1 Score: 112.464 bits (280), Expect = 4.485e-25 Identity = 95/323 (29.41%), Postives = 161/323 (49.85%), Query Frame = 1
Query: 244 QTTAELIISYC----SAFPDKVALIDDARNIKYK-FSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNL---DKDLAILPYSSGTSGLPKGVMLTHSN------FVSSNKQLEMTHE--SKESDSY-----MLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGA-ASLDQQLENRLIEKF 1146
Q E I+ C +A D + ID K K + DV+ +++++A+AL K G G +A N PE+L+ + A+ G ++ + +T E++ Q SN SI+F E+ L +V + +K V+ I + P + ++D ++ +N+ +A+LPYSSGT+G PKG LTH N S+ + E+ KE ++ +L+LP YH YG+ + + LG +V KFD + ML I+ YKV + L+PP+++FL P V F+++ K + A A+ +QL + ++F
Sbjct: 15 QPVHERILDSCRIHAAANKDAIVFIDAETTTKKKLYRDVEPTVNSLATALVKLGFKPGDVAAQAFPNCPEFLIAMLAVMKCGGAMSNASAIFTDYELQLQFKDSNTSIVFTDEDRLARVRRSVAKCPGVRKIICL-RTFPLRAEFPENVLDFVELTQTPDQPINVVVSPDAIALLPYSSGTTGRPKGCQLTHKNISAMLDIAQSHLETEVAQAMFGKEKPTWNKEHVLLLLPWYHAYGLNTMLETIL-LGATGLVFKKFDTIVMLNRIKFYKVKLAWLVPPMLIFLAKDPMVPIFNVAPYLKVIMSAGATAGKQLCEEVQKRF 335
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Match: R02E12.4 (pep chromosome:WBcel235:X:4005314:4007634:1 gene:WBGene00019828.1 transcript:R02E12.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R02E12.4) HSP 1 Score: 100.523 bits (249), Expect = 6.617e-22 Identity = 91/373 (24.40%), Postives = 180/373 (48.26%), Query Frame = -2
Query: 3198 AVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCL-VKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLK-NTSSGLAYHPGIELFDNTISIPVS-------WAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPIL 4289
AVKE + Q + T++L +RV+++ +W E+D+E+ + E ++L R L+ L+ T + +E CL V+R+ Q D D V+ L KE E + ++M ++ E Q+++N++A+ +DL K + LD K + + + + P + D P+ W + + +N+N + + R S + ++ IR A+ + V L V+ ++ L S + +EI + + +I + ++ K PL++A +R +R R +ELCND A L +E+M L+ + L+ Q A+ +L N + L++ + + +++L ID + +R +P + ++ L
Sbjct: 20 AVKEANMRAARMQGQTTQQLMQRVKDLKYWSGEIDRELADVKEEHDDLLRCYRRLQLCLDITGRSSRCNESCLAVRRKKVQVGDHTSDRVDMELHKEKELMTDTMKQMKEIGERVERQLEVNQSARKNLLRDLTLKQEAISLDHKSVAIGADGTKTVIRTPDGDRLDFREGAPLQRMSEYSEWIENTGNNLNYAARARVHSRKIGQKLCQSIREMAHQLRTEAIAVEALLKDSVRNWQEWKDNLHSQVNGKDKEIKNADGAIEEISFSLRQKSGPLQVALSRQNQRGLRPGIELCNDKAQHALHQELMMLKGTFLSLENQLDKAKESRKKLDNDRDKLQRKLDICDHNLTIDNEILRQIRTSYPHEIQLSGFL 392
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Match: CG32819 (gene:FBgn0052819 transcript:FBtr0070051) HSP 1 Score: 261.151 bits (666), Expect = 2.865e-75 Identity = 165/490 (33.67%), Postives = 269/490 (54.90%), Query Frame = -2
Query: 3210 ANST*QK*PWIRTLAVNFIQPICLWYIPMASISS-INRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4676
A T K PW +A IQ + ++P +++ + + +G + DG + P GF R PQ + AR LYTR++ N+W ++N + S R + R+++DA + ++ET+ K S Q +A +RL ER+ +++FWR EL+ E+ + E ++ E R + L + PL I++ CL RE RQG ++V D VEK+L+ E+ ++ ++++ +K Q R AQH E D+ K S +D + L N S G+ Y+ GIE FD ++S SWA+ S +++ RSQ RA LR + ++ + A +W+ +SN NN+ R QE+ A+N+++ HLQ+ QE+FD EK + LL+KAI DK PLK+A+TRLE R+ R VELC D A L +E+ +++ ++ L + AE + L+ T+ LE D+ K N+L ID++ C+ +R+ FP+ I
Sbjct: 54 AYETPTKHPWRPAMAYELIQ---VKHMPEQPVTNQLTKQCFLPKGMKT--DGMIFP-----NLVTGFDRN-------PQ--------HAARAALYTRYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQDRLVQEVQDIQGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPVSNLI 518
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Match: CG32820 (gene:FBgn0052820 transcript:FBtr0070054) HSP 1 Score: 261.151 bits (666), Expect = 2.865e-75 Identity = 165/490 (33.67%), Postives = 269/490 (54.90%), Query Frame = -2
Query: 3210 ANST*QK*PWIRTLAVNFIQPICLWYIPMASISS-INRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4676
A T K PW +A IQ + ++P +++ + + +G + DG + P GF R PQ + AR LYTR++ N+W ++N + S R + R+++DA + ++ET+ K S Q +A +RL ER+ +++FWR EL+ E+ + E ++ E R + L + PL I++ CL RE RQG ++V D VEK+L+ E+ ++ ++++ +K Q R AQH E D+ K S +D + L N S G+ Y+ GIE FD ++S SWA+ S +++ RSQ RA LR + ++ + A +W+ +SN NN+ R QE+ A+N+++ HLQ+ QE+FD EK + LL+KAI DK PLK+A+TRLE R+ R VELC D A L +E+ +++ ++ L + AE + L+ T+ LE D+ K N+L ID++ C+ +R+ FP+ I
Sbjct: 54 AYETPTKHPWRPAMAYELIQ---VKHMPEQPVTNQLTKQCFLPKGMKT--DGMIFP-----NLVTGFDRN-------PQ--------HAARAALYTRYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQDRLVQEVQDIQGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPVSNLI 518
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Match: CG17450 (gene:FBgn0040028 transcript:FBtr0345920) HSP 1 Score: 261.151 bits (666), Expect = 2.865e-75 Identity = 165/490 (33.67%), Postives = 269/490 (54.90%), Query Frame = -2
Query: 3210 ANST*QK*PWIRTLAVNFIQPICLWYIPMASISS-INRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4676
A T K PW +A IQ + ++P +++ + + +G + DG + P GF R PQ + AR LYTR++ N+W ++N + S R + R+++DA + ++ET+ K S Q +A +RL ER+ +++FWR EL+ E+ + E ++ E R + L + PL I++ CL RE RQG ++V D VEK+L+ E+ ++ ++++ +K Q R AQH E D+ K S +D + L N S G+ Y+ GIE FD ++S SWA+ S +++ RSQ RA LR + ++ + A +W+ +SN NN+ R QE+ A+N+++ HLQ+ QE+FD EK + LL+KAI DK PLK+A+TRLE R+ R VELC D A L +E+ +++ ++ L + AE + L+ T+ LE D+ K N+L ID++ C+ +R+ FP+ I
Sbjct: 54 AYETPTKHPWRPAMAYELIQ---VKHMPEQPVTNQLTKQCFLPKGMKT--DGMIFP-----NLVTGFDRN-------PQ--------HAARAALYTRYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQDRLVQEVQDIQGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPVSNLI 518
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Match: CG17450 (gene:FBgn0040028 transcript:FBtr0070056) HSP 1 Score: 261.151 bits (666), Expect = 2.865e-75 Identity = 165/490 (33.67%), Postives = 269/490 (54.90%), Query Frame = -2
Query: 3210 ANST*QK*PWIRTLAVNFIQPICLWYIPMASISS-INRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4676
A T K PW +A IQ + ++P +++ + + +G + DG + P GF R PQ + AR LYTR++ N+W ++N + S R + R+++DA + ++ET+ K S Q +A +RL ER+ +++FWR EL+ E+ + E ++ E R + L + PL I++ CL RE RQG ++V D VEK+L+ E+ ++ ++++ +K Q R AQH E D+ K S +D + L N S G+ Y+ GIE FD ++S SWA+ S +++ RSQ RA LR + ++ + A +W+ +SN NN+ R QE+ A+N+++ HLQ+ QE+FD EK + LL+KAI DK PLK+A+TRLE R+ R VELC D A L +E+ +++ ++ L + AE + L+ T+ LE D+ K N+L ID++ C+ +R+ FP+ I
Sbjct: 54 AYETPTKHPWRPAMAYELIQ---VKHMPEQPVTNQLTKQCFLPKGMKT--DGMIFP-----NLVTGFDRN-------PQ--------HAARAALYTRYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQDRLVQEVQDIQGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPVSNLI 518
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Match: CG17450 (gene:FBgn0040028 transcript:FBtr0070057) HSP 1 Score: 260.381 bits (664), Expect = 3.547e-74 Identity = 165/490 (33.67%), Postives = 269/490 (54.90%), Query Frame = -2
Query: 3210 ANST*QK*PWIRTLAVNFIQPICLWYIPMASISS-INRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4676
A T K PW +A IQ + ++P +++ + + +G + DG + P GF R PQ + AR LYTR++ N+W ++N + S R + R+++DA + ++ET+ K S Q +A +RL ER+ +++FWR EL+ E+ + E ++ E R + L + PL I++ CL RE RQG ++V D VEK+L+ E+ ++ ++++ +K Q R AQH E D+ K S +D + L N S G+ Y+ GIE FD ++S SWA+ S +++ RSQ RA LR + ++ + A +W+ +SN NN+ R QE+ A+N+++ HLQ+ QE+FD EK + LL+KAI DK PLK+A+TRLE R+ R VELC D A L +E+ +++ ++ L + AE + L+ T+ LE D+ K N+L ID++ C+ +R+ FP+ I
Sbjct: 135 AYETPTKHPWRPAMAYELIQ---VKHMPEQPVTNQLTKQCFLPKGMKT--DGMIFP-----NLVTGFDRN-------PQ--------HAARAALYTRYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQDRLVQEVQDIQGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPVSNLI 599
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2]) HSP 1 Score: 346.665 bits (888), Expect = 1.768e-105 Identity = 195/480 (40.62%), Postives = 290/480 (60.42%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQ--------VKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
T + +P ++P +IS+ S + S N S P R +N + R+ S+I V+ ++Y + R L TR+ P DW SN ++++ SE+ R A RL+ D + +++ E TR Q +K + ER+ +ISFWR E++ EI +M E L + R L+ L T+ PL +++ CL RE R ID V D+VE LIKEV+ IK CQE+M R L++ Q+ NRAAQHE E+DL DK + ++DD+ +HL+NTS G++Y+ GI+ D +IS+P SW+KF+ DNI SQ RA+S LR EI++L+ +N MWN F++VN S +R+ E + +N L++HL +T+QEI+ E I L+KAI DKE PLK+A+TRLEERT+R NVELC D L E+ E+ ++I L+++ AE L+ TK LE D+++K NSL +DQ+ C+ +RK FP
Sbjct: 7 TQMATYTRPKTSHFLP--AISTTASSYRSHQPPLSLNQNSNMPWR-----TNSYYRSGSAIPTLSALQRDNLELVRAKTMFYPSNRTALSTRYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHHRLITEVREIEDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSFP 479
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2]) HSP 1 Score: 346.665 bits (888), Expect = 1.768e-105 Identity = 195/480 (40.62%), Postives = 290/480 (60.42%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQ--------VKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
T + +P ++P +IS+ S + S N S P R +N + R+ S+I V+ ++Y + R L TR+ P DW SN ++++ SE+ R A RL+ D + +++ E TR Q +K + ER+ +ISFWR E++ EI +M E L + R L+ L T+ PL +++ CL RE R ID V D+VE LIKEV+ IK CQE+M R L++ Q+ NRAAQHE E+DL DK + ++DD+ +HL+NTS G++Y+ GI+ D +IS+P SW+KF+ DNI SQ RA+S LR EI++L+ +N MWN F++VN S +R+ E + +N L++HL +T+QEI+ E I L+KAI DKE PLK+A+TRLEERT+R NVELC D L E+ E+ ++I L+++ AE L+ TK LE D+++K NSL +DQ+ C+ +RK FP
Sbjct: 7 TQMATYTRPKTSHFLP--AISTTASSYRSHQPPLSLNQNSNMPWR-----TNSYYRSGSAIPTLSALQRDNLELVRAKTMFYPSNRTALSTRYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHHRLITEVREIEDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSFP 479
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2]) HSP 1 Score: 345.125 bits (884), Expect = 4.114e-105 Identity = 192/472 (40.68%), Postives = 283/472 (59.96%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
T + +P ++P S ++ N P R GS P L+ N V+ ++Y + R L TR+ P DW SN ++++ SE+ R A RL+ D + +++ E TR Q +K + ER+ +ISFWR E++ EI +M E L + R L+ L T+ PL +++ CL RE R ID V D+VE LIKEV+ IK CQE+M R L++ Q+ NRAAQHE E+DL DK + ++DD+ +HL+NTS G++Y+ GI+ D +IS+P SW+KF+ DNI SQ RA+S LR EI++L+ +N MWN F++VN S +R+ E + +N L++HL +T+QEI+ E I L+KAI DKE PLK+A+TRLEERT+R NVELC D L E+ E+ ++I L+++ AE L+ TK LE D+++K NSL +DQ+ C+ +RK FP
Sbjct: 7 TQMATYTRPKTSHFLPAISTTNSNMPW---RTNSYYRSGSAIPTLSALQRDN-----------LELVRAKTMFYPSNRTALSTRYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHHRLITEVREIEDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSFP 464
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Match: tekt1 (tektin 1 [Source:ZFIN;Acc:ZDB-GENE-041114-99]) HSP 1 Score: 186.037 bits (471), Expect = 1.102e-50 Identity = 130/386 (33.68%), Postives = 211/386 (54.66%), Query Frame = -2
Query: 3234 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRK 4391
RF P++W+ +N H++ SEA R + +L ++ + ++E + Q + KRL R+Q+I FWRQEL Q+ M E E L + +++ LE +P ++ CL +R+ R ID V DEVE+ L+KE E ++ + R L++ Q+++ R+ ++ EKDL+DK + +DD L NTS L + P W NI++++K + +S +LR +D L+ A M + + R++E A+ +LE+ L + EI + EK+ + LR A+A+KE PLK+A+TRL R++R NVELC+DP L +I EL N ++ L E +E L + LE++I VK NSL ID+ C +R+
Sbjct: 10 RFLPSEWKHANQEHYRSSEAGRKHSEKLTAECERLIEECNKSNKRMQHDTQKRLERRIQDIKFWRQELVQKFEQMVQEIETLIIYKSRVEKALESCSEPFQVTLQCLAERQKRVAIDLVHDEVEEELLKEKEVVEGVMVLLQRTLEQINEQIRLIRSVKYYLEKDLQDKFQAEHIDDFCSLLTNTSPDLNEGNSSFKYSGAAVTPEEWESLCNLNISKAEKEKNNSLSLRALVDSLLEQTARDMRRQYETTGRAFDLRIEETKTAKEQLENQLNELLAEIANQEKNQDALRLAVAEKEAPLKVAQTRLSTRSQRPNVELCHDPPQIQLLAQIKELTNHVQRLSEALELSESELKALAYNQLRLEEEIQVKTNSLYIDEVICHQLRQ 395
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Match: tekt4 (tektin 4 [Source:ZFIN;Acc:ZDB-GENE-070410-98]) HSP 1 Score: 178.333 bits (451), Expect = 1.532e-47 Identity = 139/458 (30.35%), Postives = 235/458 (51.31%), Query Frame = -2
Query: 3225 MASISSINRPIVSARGRYSNNDGSVSPVRFP--LRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4592
M+S ++RP R GS PV P N ++ T + + T Y +A+ ++P++W +N+A + A+R QA + ++ ET+ T Q E T L R+Q+I WR EL++ I + E + R L++ L+ T+ P AI+ L RE R G D V+D VE+ L+KEVE I+ Q + + L + Q+++NR +H E D DK ++ LDD N S+ HP + + + W KF+ DN+ +++ +S+ALR + +++ + V+ + R E++ A+ +LE HL + +++I E++I+ L++A+ DKE PL++A++RL RT R N+E C D +L E +L N++ L++Q A+ + L ++ LEKDIA K NSL ID++ C+ R +P
Sbjct: 2 MSSQILVSRPHYDTR--VVAQSGSQPPVENPEIAVKHNVYAGTSTGL--------TTAGYRSAK------YTPDEWFDNNSALLNRAAADRNQAENICHESRALKAETDAATLRTQTEGTTHLGARLQDIHLWRSELERLIERLSAETDLQISSRRRLEKALDATEIPFAIATDNLTCRERRFGPDLVKDAVEEELLKEVELIRSIQTLLKKTLDQTINQIRLNREVKHTLELDWSDKQQAYSLDDHCGRFSNRSTDTQRHPSSAVLQDQVCDEEVWRKFTVDNLRLAEREETASQALRRLTERVLQETTEDLRAQCDAVDRAFTQRCVEMSQAKTQLELHLAQILEQIGAQERNISQLQEAVHDKEAPLRVAQSRLHYRTLRPNMEQCRDQPQLSLLGEASKLSNTVSALQQQLYEAQKSLSDLEESRLNLEKDIACKTNSLLIDREKCMTHRTRYP 443
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:549229]) HSP 1 Score: 359.377 bits (921), Expect = 7.935e-110 Identity = 198/464 (42.67%), Postives = 291/464 (62.72%), Query Frame = -2
Query: 3225 PMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTK-------IYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4595
P + +I+ S R R+ + S S + P R S + ++ S+ P K++ + + + R L+TR++P+DW SN +++ SE+ R A RL+ D + ++E + KTR Q++ K L ERV +ISFW+ E+ EI M E L E R ++ L T+ PL +S+ CL RE R ID V D+VEK LI+EV+ IK CQEKM R LQK Q+ NRAAQHE E+D+ DK + ++DDK +L+N+S G+ Y G+E D T+SIP +WAK+S DNI RSQ R +S LR + + ++ + +N MW F+ VN + SR+ E +A+NKL++HL +T+QEIF E +I ++KAI DK PLK+A+TRL+ERT+R NVELC D L +E+ ++ N+I+ L+ + R AE LI+TKS+LE D+A+K NSL IDQ+ C+G+RK FP
Sbjct: 19 PTNFLPAISTMSASYRNRFPHYTMSHS-LTLPWRPSTYYKVASANPSLAPLSKSSPGLNPSQILSFPSNRTALFTRYTPDDWYKSNQVNYRDSESSRRSAERLRVDTSRLIQEKDQKTRKVQSDTNKDLGERVNDISFWKSEIIHEIDQMIGETNALSEMKRRMERALMETEGPLQVSQECLYHREKRMSIDLVHDDVEKQLIQEVDCIKSCQEKMRRHLQKISAQLASNRAAQHELERDISDKQTALRIDDKCQNLRNSSDGIGYFRGVERMDATVSIPETWAKYSDDNILRSQSDRVASTKLREDAETILASTSNDMWGQFNAVNVNFTSRISETADAKNKLQNHLAKTLQEIFQTEMTIEAIKKAIKDKSAPLKVAQTRLDERTRRPNVELCRDSVQMRLIQEVQDIDNTIQALQLRLREAEDALQMLIHTKSILEHDLAIKANSLFIDQEKCMGMRKSFP 481
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Match: gdf10 (growth differentiation factor 10 [Source:Xenbase;Acc:XB-GENE-5998698]) HSP 1 Score: 241.121 bits (614), Expect = 1.906e-69 Identity = 141/397 (35.52%), Postives = 237/397 (59.70%), Query Frame = -2
Query: 3219 QLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSM--DIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMD 4403
QL +F P +W +N + +E++R+++ RL +++ + V E E T++ Q + K+L++R++ I FW++ELD ++ DI Q L + L++ LE ++PL+I++ CL++RE R GID V DE E+ LIKEVE I+ + R +++ Q++M+R+A++ EKDLKDK + ++D+ L N S + Y + P+ W ++ NI +++K R ++ ALR ID ++ A+ + +L +R+QE +A+NKLE HL + + EI EK+I +L+KA+ADKE P+K+A+TRL+ RT R NVELC DP L E+ E+ ++ L+E AE L + LE++I VKENS+ ID+ C+ +R+ ++
Sbjct: 6 QLPPKFLPKEWNIANKTCYARAESQRSRSERLVAESQRLVDEIEKTTQNSQKDVNKKLDQRLEEIQFWKKELDSKLEDTVNDIAQ--LLAYKIRLEKALESCKEPLSIAQQCLIEREKRVGIDLVHDEAERELIKEVEVIQGVMTLLERTIEQITEQIRMSRSAKYSLEKDLKDKFQALKIDNHCAGLTNNSPDIDYSANTVRIEANSVTPIEWENYTNTNILKAEKQRNNAIALRSLIDGILSETASDICKQCDTATYALKNRIQETKDAKNKLEEHLAKVMDEISSQEKNIEILKKALADKEGPMKMAQTRLDTRTLRPNVELCRDPVQYRLINEVQEITTNVERLRETLAQAEAELKALNRNQLSLEEEIQVKENSIYIDEVICMQMREAISIN 400
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Match: plcd1 (phospholipase C delta 1 [Source:Xenbase;Acc:XB-GENE-1013054]) HSP 1 Score: 231.876 bits (590), Expect = 1.209e-65 Identity = 156/475 (32.84%), Postives = 258/475 (54.32%), Query Frame = -2
Query: 3210 PICLWYIP--MASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQV-KNTKIYYNTARNQLYTR---FSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4616
PI LW + M+S ++RP+ SV P++S PQV +NT Y ++ R + +W+ N A F + ++R Q+ + +A + V ETE + + QA+ TK L ER+ +I FW+ EL +EIR ++ E + L + L+ L+ T P +I+ L R+ R+G + V+DEVE L+KEV+ I+ QE + R L + Q++ NR A+ E D DK +++ DDK N S+ + +H +++ S P SWA+++ +NI ++++ R +S LR ID ++++ + + + + R +E+ +A+ KLE HL++T++EI EK+I L++AI DK PLK+A+TRL +R+ R NVELC D L E+ EL +S+ LK + +E L +T+ LEKDIA K NS+ ID++ C+ +R +P K+
Sbjct: 5 PIRLWKLTTDMSSQILVSRPLAPQ---------SVPATSLPVQS--------------PQVAQNTGPYSSSGLATAGFRTAKYLHEEWQQGNLATFYRAFSDRDQSETGRHEAKQLVTETEARAQRSQADCTKSLGERLLDIHFWKSELAREIRDVEAETQLLVQQKGRLERALDATDIPFSIATDNLKCRDRRRGSELVRDEVELELLKEVDLIRNVQELLKRTLDQTVQQIRQNRDAKENLEMDYSDKVEAYEFDDKCGRYNNQSTDIQFHRNSSKYEDNTSTPESWAQYTHENIYKAERERMASINLRSLIDSILQDISEDLEAQSAAIAVDFEKRCREVEDAKQKLEMHLKKTLEEIGGQEKNIAALKQAINDKSPPLKVAQTRLHDRSYRPNVELCRDHVQIRLVSEVGELTDSLDALKLKLEDSEQSLRNLEDTRMSLEKDIANKANSIFIDREKCMTLRTRYPHGMKL 456
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Match: fam168a (family with sequence similarity 168 member A [Source:Xenbase;Acc:XB-GENE-5856038]) HSP 1 Score: 147.902 bits (372), Expect = 2.928e-38 Identity = 100/296 (33.78%), Postives = 163/296 (55.07%), Query Frame = -2
Query: 3435 RLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSM--DIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRA-AQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVEL 4313
RL +++ + V E+E T+ Q + K+L++R++ I FW+ ELD ++ DI Q L R L+++LE ++PL++++ CL+K+E R GID V DE E+ LIK V+ I+ ++ NR +++ EKDLKDK + ++D+ L N S + Y + P+ W F+ NI +++K R ++ ALR ID ++ A+ ++ A+NKLE HL + + EI EK+I +L+KA+ADKE P+K+ + RL+ RT R NVEL
Sbjct: 18 RLVAESQRLVDESEKTTQKSQKDFNKKLDQRLEEIQFWK-ELDSKLEDTVNDIAQL-LACKFR-LEKSLERCKEPLSLAQQCLIKQEKRVGIDLVHDEAEQELIKAVDVIQGVM-----------TLLEYNRTNSKYSLEKDLKDKFQALKIDNHCAGLTNNSPDIGYSANTVRIEANSVTPIEWEDFTNTNIVKAEKRRNNAVALRSLIDGILSETASDIY-------------------AKNKLEEHLAKVISEISSQEKNIEILKKALADKEGPMKMEQIRLDTRTLRPNVEL 280
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Match: fam168a (family with sequence similarity 168 member A [Source:Xenbase;Acc:XB-GENE-5856038]) HSP 1 Score: 144.821 bits (364), Expect = 1.947e-37 Identity = 96/279 (34.41%), Postives = 154/279 (55.20%), Query Frame = -2
Query: 3435 TRSRQAEATKRLNERVQNISFWRQELDQEIRSM--DIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVEL 4265
T+ Q + K+L++R++ I FW+ ELD ++ DI Q L R L+++LE ++PL++++ CL+K+E R GID V DE E+ LIK V+ I Q ++M+ +A++ EKDLKDK + ++D+ L N S + Y + P+ W F+ NI +++K R ++ ALR ID ++ A+ ++ A+NKLE HL + + EI EK+I +L+KA+ADKE P+K+ + RL+ RT R NVEL
Sbjct: 1 TQKSQKDFNKKLDQRLEEIQFWK-ELDSKLEDTVNDIAQL-LACKFR-LEKSLERCKEPLSLAQQCLIKQEKRVGIDLVHDEAEQELIKAVDVI-----------QGVMTLLEMSLSAKYSLEKDLKDKFQALKIDNHCAGLTNNSPDIGYSANTVRIEANSVTPIEWEDFTNTNIVKAEKRRNNAVALRSLIDGILSETASDIY-------------------AKNKLEEHLAKVISEISSQEKNIEILKKALADKEGPMKMEQIRLDTRTLRPNVEL 246
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Match: Tekt3 (tektin 3 [Source:MGI Symbol;Acc:MGI:1918312]) HSP 1 Score: 323.553 bits (828), Expect = 3.517e-97 Identity = 186/482 (38.59%), Postives = 285/482 (59.13%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASIS---SINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQ-------VKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P AS S +I S + R+ + + + S + P R + + + ++ P ++T + + + R +TR++P+DW SN F+ S + R + RL+ D + +++ + R QA +T+ L ERV +++FW+ E+ E+ M E L + R L+ L T+ PL +S CL RE R GID V DE EK L+ EV+TI CQE+M + L K Q+ +R+AQHE EKDL DK + ++DDK HL+NTS G++Y G+E D T+S+P +WAKF+ DN+ RSQ RA+S LR E + L+ AN MWN F+ VN + +R+ E +A+NK+ +HL +T+QEIF E +I ++KAI +K LK+A+TRL+ERT+R NVELC D A L E+ E+ +I+ L+++ R +E L +TK+ LE D+AVK N+L IDQ+ C+ +R +P
Sbjct: 7 TLTATYAHP------PPASASFLPAIGTITSSYKDRFPHRNLTHS-LSLPWRPNTYYKTAYNYPTLAPYSSRSQRVCESTMLPFVSNRTTFFTRYTPDDWYRSNLVSFQESNSSRHNSERLRVDTSRLIQDKYQQIRKTQAHSTQNLGERVNDLAFWKSEITHELDEMIGETNALTDIKRRLERGLIETEGPLQVSRECLFHREKRMGIDLVHDEAEKELLAEVDTILCCQERMRQHLDKANAQLASDRSAQHELEKDLSDKQAALRIDDKCQHLRNTSEGVSYFRGVERVDATVSVPETWAKFTDDNVLRSQSERAASAKLREETENLLIVTANEMWNQFNKVNLAFTNRIAETVDAKNKIHTHLTKTLQEIFQIEMTIESIKKAIKEKSAFLKVAQTRLDERTRRPNVELCRDMAQLRLVNEVYEVDETIQTLQQRLRDSEDTLQSLAHTKATLEHDLAVKANTLYIDQEKCMSMRNSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Match: Tekt5 (tektin 5 [Source:MGI Symbol;Acc:MGI:1917676]) HSP 1 Score: 281.567 bits (719), Expect = 9.055e-82 Identity = 150/395 (37.97%), Postives = 243/395 (61.52%), Query Frame = -2
Query: 3225 RNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4409
R+ L+ R++P DW+ SN+ + +EA R A+RL D+ + +++ + Q ++ L +R+ ++ FW+ EL E+ + E ++ R L+ E PL ++ CL RE R GID V D VEK+LI+EV+ +K CQ++M + ++ + Q++ NR AQH E+D++DKS + +D+ ++L++TS +++ G+E FD T+SIP +WAKFS DNI +Q RA+S LR E + L ++ MW F+N N + +R+ E T+ +NKL++ L + +QEIF E +I LL +AI KE PLK+A+T L RT+R NVELC D L E+ + ++++ LK + R + L+ TKS LE ++A+K N+L ID+ C+ +RK FP
Sbjct: 83 RSALFCRYTPRDWDRSNDLQIRNAEASRLWASRLTGDSLRIMQDKDQLIHQMQEGTSRNLGQRLSDLGFWKSELCYELDRLLTENSSMDTLKRRLECAAEEVNCPLQVALECLYNREKRIGIDLVHDNVEKNLIREVDLLKCCQDQMRKLAKRIDFQIRDNRDAQHSLERDIEDKSSAQYIDENCFNLRSTSDSISFFHGVEKFDGTVSIPETWAKFSNDNIRHAQNMRANSIRLREEAEHLFETLSDQMWKQFTNTNLAFNARISEETDVKNKLQTQLAKILQEIFQAENTIMLLERAIVAKEYPLKMAQTMLACRTRRPNVELCRDVPQFRLVNEVFTIDDTLQTLKLRLRETQDTLQLLVMTKSRLEHELAIKANTLCIDKDKCMSMRKSFP 477
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Match: Tekt5 (tektin 5 [Source:MGI Symbol;Acc:MGI:1917676]) HSP 1 Score: 244.588 bits (623), Expect = 1.346e-70 Identity = 130/333 (39.04%), Postives = 207/333 (62.16%), Query Frame = -2
Query: 3414 RNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCND-PAMR 4409
R+ L+ R++P DW+ SN+ + +EA R A+RL D+ + +++ + Q ++ L +R+ ++ FW+ EL E+ + E ++ R L+ E PL ++ CL RE R GID V D VEK+LI+EV+ +K CQ++M + ++ + Q++ NR AQH E+D++DKS + +D+ ++L++TS +++ G+E FD T+SIP +WAKFS DNI +Q RA+S LR E + L ++ MW F+N N + +R+ E T+ +NKL++ L + +QEIF E +I LL +AI KE PLK+A+T L RT+R NVELC D P R
Sbjct: 83 RSALFCRYTPRDWDRSNDLQIRNAEASRLWASRLTGDSLRIMQDKDQLIHQMQEGTSRNLGQRLSDLGFWKSELCYELDRLLTENSSMDTLKRRLECAAEEVNCPLQVALECLYNREKRIGIDLVHDNVEKNLIREVDLLKCCQDQMRKLAKRIDFQIRDNRDAQHSLERDIEDKSSAQYIDENCFNLRSTSDSISFFHGVEKFDGTVSIPETWAKFSNDNIRHAQNMRANSIRLREEAEHLFETLSDQMWKQFTNTNLAFNARISEETDVKNKLQTQLAKILQEIFQAENTIMLLERAIVAKEYPLKMAQTMLACRTRRPNVELCRDVPQFR 415
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Match: Tekt1 (tektin 1 [Source:MGI Symbol;Acc:MGI:1333819]) HSP 1 Score: 222.246 bits (565), Expect = 7.072e-63 Identity = 137/390 (35.13%), Postives = 228/390 (58.46%), Query Frame = -2
Query: 3225 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFD-NTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4391
RF P +W +N + + +EA+R+Q+ RL +++ + V+E E TR Q++ K+L +R++ + FW++ELD ++ + + ++L + L+ +LE ++PL I+E CL RE R GID V D VE+ L KE + I + R L++ Q+++NR+A++ EKDL+DK + +DD + L N S + + + + N++S+ W FS N+ ++ K +S AL+ +D ++ AN + V+ + ++ ++E +ARNKL HL + ++EI EK+I L AI +E P K+A TRLE RT R NVELC D A L KEI E+ +++ LKE A+ L + L+++I VKEN++ IDQ C+ +RK P
Sbjct: 10 RFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLE-DWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQYRLIKEIQEINHNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSIP 398
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Match: Tekt1 (tektin 1 [Source:MGI Symbol;Acc:MGI:1333819]) HSP 1 Score: 222.246 bits (565), Expect = 7.072e-63 Identity = 137/390 (35.13%), Postives = 228/390 (58.46%), Query Frame = -2
Query: 3225 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFD-NTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4391
RF P +W +N + + +EA+R+Q+ RL +++ + V+E E TR Q++ K+L +R++ + FW++ELD ++ + + ++L + L+ +LE ++PL I+E CL RE R GID V D VE+ L KE + I + R L++ Q+++NR+A++ EKDL+DK + +DD + L N S + + + + N++S+ W FS N+ ++ K +S AL+ +D ++ AN + V+ + ++ ++E +ARNKL HL + ++EI EK+I L AI +E P K+A TRLE RT R NVELC D A L KEI E+ +++ LKE A+ L + L+++I VKEN++ IDQ C+ +RK P
Sbjct: 10 RFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLE-DWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQYRLIKEIQEINHNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSIP 398
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Match: sp|A6H782|TEKT3_BOVIN (Tektin-3 OS=Bos taurus OX=9913 GN=TEKT3 PE=1 SV=1) HSP 1 Score: 342.813 bits (878), Expect = 3.963e-103 Identity = 191/479 (39.87%), Postives = 292/479 (60.96%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQV-------KNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P + +I+ + R R+ + + + S + P R S + + ++ P ++T + + + R L+TR++P+DW SN +F+ S R + RL+ D + +++ +TR QA++T+ L ERV +I FW+ E+ E+ +M E L + + L+ L T+ PL ++ CL RE R GID V DEVEK L+ EV+TI CQE+M +L K Q+ NRAAQHE EKDL DK ++++DDK +HL+NTS G++Y G+E D T+S+P SWAKF+ DNI RSQ RA+S LR +I ++ AN MWN F+ VN + +R+ E +A+NK+++HL +T+QEIF E +I ++KAI +K LK+A+TRL+ERT+R N+ELC D A L E+ E+ ++I+ L+++ R AE L +TK+ LE D+AVK NSL IDQ C+ +R+ FP
Sbjct: 7 TLTATYAHPRP---TPTNFLPAISTMASTYRDRFPHYNLTHS-LSLPWRPSTYYKAASNWPTLDPYCTRSQRVSESTMLPFVSNRTTLFTRYTPDDWYRSNLTNFQESNTSRHNSERLRVDTSRLIQDKYQQTRKTQADSTQNLGERVNDIGFWKSEIIHELDAMIGETNELTDIKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEKELLTEVDTILCCQERMKLYLDKAIAQLAANRAAQHELEKDLSDKQSAYRIDDKCHHLRNTSDGVSYFHGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIQNVLVVTANEMWNQFNKVNLAFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIVEKSAFLKVAQTRLDERTRRPNIELCRDMAQLRLVNEVYEVDDTIQTLQQRLRDAEDTLQSLAHTKATLEHDLAVKANSLYIDQDKCMSMRRSFP 481
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Match: sp|Q9BXF9|TEKT3_HUMAN (Tektin-3 OS=Homo sapiens OX=9606 GN=TEKT3 PE=1 SV=1) HSP 1 Score: 342.428 bits (877), Expect = 5.436e-103 Identity = 193/479 (40.29%), Postives = 290/479 (60.54%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQV-------KNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P + +I+ S R R+ +++ + S + P R S + +S SV P +NT + + + R +TR++P+DW SN +++ S R + +L+ D + +++ +TR QA+ T+ L ERV +I FW+ E+ E+ M E L + + L+ L T+ PL ++ CL RE R GID V DEVE L+ EV+TI CQE+M L K Q+ NRA+QHE EKDL DK ++++DDK +HL+NTS G+ Y G+E D T+S+P SWAKF+ DNI RSQ RA+S LR +I+ L+ AN MWN F+ VN S +R+ E +A+NK+++HL +T+QEIF E +I ++KAI DK LK+A+TRL+ERT+R N+ELC D A L E+ E+ ++I+ L+++ R AE L++ K+ LE D+AVK NSL IDQ+ C+ +RK +P
Sbjct: 7 TLTTTYAHPRP---TPTNFLPAISTMASSYRDRFPHSNLTHS-LSLPWRPSTYYKVASNSPSVAPYCTRSQRVSENTMLPFVSNRTTFFTRYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQADTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRIDDKCHHLRNTSDGVGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQLRLVNEVHEVDDTIQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Match: sp|Q6X6Z7|TEKT3_MOUSE (Tektin-3 OS=Mus musculus OX=10090 GN=Tekt3 PE=2 SV=1) HSP 1 Score: 323.553 bits (828), Expect = 2.460e-96 Identity = 186/482 (38.59%), Postives = 285/482 (59.13%), Query Frame = -2
Query: 3225 TLAVNFIQPICLWYIPMASIS---SINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQ-------VKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4640
TL + P P AS S +I S + R+ + + + S + P R + + + ++ P ++T + + + R +TR++P+DW SN F+ S + R + RL+ D + +++ + R QA +T+ L ERV +++FW+ E+ E+ M E L + R L+ L T+ PL +S CL RE R GID V DE EK L+ EV+TI CQE+M + L K Q+ +R+AQHE EKDL DK + ++DDK HL+NTS G++Y G+E D T+S+P +WAKF+ DN+ RSQ RA+S LR E + L+ AN MWN F+ VN + +R+ E +A+NK+ +HL +T+QEIF E +I ++KAI +K LK+A+TRL+ERT+R NVELC D A L E+ E+ +I+ L+++ R +E L +TK+ LE D+AVK N+L IDQ+ C+ +R +P
Sbjct: 7 TLTATYAHP------PPASASFLPAIGTITSSYKDRFPHRNLTHS-LSLPWRPNTYYKTAYNYPTLAPYSSRSQRVCESTMLPFVSNRTTFFTRYTPDDWYRSNLVSFQESNSSRHNSERLRVDTSRLIQDKYQQIRKTQAHSTQNLGERVNDLAFWKSEITHELDEMIGETNALTDIKRRLERGLIETEGPLQVSRECLFHREKRMGIDLVHDEAEKELLAEVDTILCCQERMRQHLDKANAQLASDRSAQHELEKDLSDKQAALRIDDKCQHLRNTSEGVSYFRGVERVDATVSVPETWAKFTDDNVLRSQSERAASAKLREETENLLIVTANEMWNQFNKVNLAFTNRIAETVDAKNKIHTHLTKTLQEIFQIEMTIESIKKAIKEKSAFLKVAQTRLDERTRRPNVELCRDMAQLRLVNEVYEVDETIQTLQQRLRDSEDTLQSLAHTKATLEHDLAVKANTLYIDQEKCMSMRNSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Match: sp|Q4V8G8|TEKT3_RAT (Tektin-3 OS=Rattus norvegicus OX=10116 GN=Tekt3 PE=1 SV=1) HSP 1 Score: 323.553 bits (828), Expect = 2.484e-96 Identity = 188/485 (38.76%), Postives = 284/485 (58.56%), Query Frame = -2
Query: 3225 PWIRTLAVNFI--QPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFP-------QVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4652
P TL + QP ++P +I S R R+ + + + S + P R S + + ++ P ++T + + + R L+TR++P+DW S F+ S R + RL+ D + +++ + R QA++T+ L ERV +I+FW+ E+ E+ M E L + R L+ L T PL ++ CL RE R GID V DE EK L+ EVET+ CQE+M R L K Q+ +R+AQHE EKDL DK + ++DDK HL+NTS G++Y G+E D T+S+P SWAKF+ DN+ RSQ RA+S LR + + L+ AN MWN F+ VN + +R+ E +A+NK+ HL +T+QEIF E +I +RKAI +K LK+A+TRL+ERT+R N+ELC D A L E+ E+ +I+ L+++ R +E L +TK+ LE D+AVK N+L IDQ+ C+ +R +P
Sbjct: 3 PLGSTLTATYAHPQPAATNFLP-----AIGTLTSSYRNRFPHRNLTHS-LSLPWRPSTYYKTAYNYPTLAPLSSTSQSVCESTMLPFVSNRTTLFTRYTPDDWYRSTLVGFQESNCSRHNSERLRVDTSRLIQDKYQQIRKTQADSTQNLGERVNDIAFWKSEIIHELDEMIGETNALTDIKRRLERGLIETDAPLQVARECLFHREKRMGIDLVHDEAEKELLTEVETVLCCQERMRRHLDKAIAQLASDRSAQHELEKDLSDKQAALRIDDKCKHLRNTSQGVSYFRGVENVDATVSVPESWAKFTDDNVLRSQSERAASAKLREDTENLLIVIANEMWNQFNKVNVAFTNRIAETVDAKNKIHIHLSKTLQEIFQTEMAIESIRKAIKEKSAFLKVAQTRLDERTRRPNIELCRDIAQLRLVNEVYEVDETIQTLQQRLRDSEDTLQSLAHTKATLEHDLAVKANTLYIDQEKCMSMRNSYP 481
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Match: sp|Q96M29|TEKT5_HUMAN (Tektin-5 OS=Homo sapiens OX=9606 GN=TEKT5 PE=1 SV=1) HSP 1 Score: 306.22 bits (783), Expect = 2.494e-90 Identity = 162/402 (40.30%), Postives = 254/402 (63.18%), Query Frame = -2
Query: 3210 TARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKI 4415
T R+ L++R+SP+DW+ SN + +EA R A+RL D+ + +++ + T Q + L +R+ +I FW+ EL E+ + E +NL+ R L+ PL ++ CL RE R GID V D VEK+LI+EV+ +K CQE+M + Q+ +IQM+ NR AQH E+DL+DKS + +D+K ++L+NTS +++ G+E D TIS+P +WAKFS DNI SQ RA+S LR E + L ++ MW F++ N + +R+ E+T+ +NKL++ L +T+QEIF E +I LL ++I KE PLK+A+TRLE RT+R N+ELC D L E+ + ++++ LK + R + L+ TK LE ++A+K N+L ID++ C+G+RK FP ++
Sbjct: 80 TLRSALFSRYSPHDWDQSNQLQVRGAEASRLWASRLTDDSMRLLQDKDQLTHQMQEGTCRNLGQRLSDIGFWKSELSYELDRLLTENQNLETVKRRLECAANEVNCPLQVALECLYHREKRIGIDLVHDNVEKNLIREVDLLKCCQEQMRKLAQRIDIQMRDNRDAQHVLERDLEDKSSAQCIDEKCFNLRNTSDCISFFHGMEKIDGTISVPETWAKFSNDNIKHSQNMRANSIQLREEAEHLFETLSDQMWRQFTDTNLAFNARISEVTDVKNKLQTQLAKTLQEIFQAENTIMLLERSIMAKEGPLKVAQTRLECRTRRPNMELCRDIPQLKLVNEVFTIDDTLQTLKLRLRETQDTLQLLVMTKCRLEHELAIKANTLCIDKEKCMGMRKTFPCTPRL 481
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Match: A0A1I8GUU6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004034g1 PE=4 SV=1) HSP 1 Score: 535.028 bits (1377), Expect = 4.966e-172 Identity = 261/447 (58.39%), Postives = 349/447 (78.08%), Query Frame = -2
Query: 3183 SVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLA 4523
S+S P+ +R S ++ P V + + +YN ARN +Y RFS +DW+ SN A+F +SE ERA+A RL+++AW+ V+ T+ +T++RQ++A+KRLNER+++I FWR+EL +E + E ENL EHLRVL+ L+HT KPL ISE CL+ RE R GIDQV D VE+ LIKEVE IK+CQ M + + K ++Q+K+NRAA HE +KD+K KSHS ++DD+MYHL++ SSG+AYHPGIE DN+ S+P SWA+FSQ+NI RSQKCRA+SE LRG ID L+R CAN MW+ FS VNN+L SRV+E T+A+NKL+++LQ+T+ EIFD ++SI LLRKAIADKE P+K+A+TRLEERT+RL++E+CNDPAM TLQ+E+ E+R+SIR LK++ R+AE +RL TK LLE DIAVKENS+QID K CLG+RK FPMD K+GPI MP+A
Sbjct: 23 SLSRALPPIAQETVGNRLSRSYTLPPGVSHAQNFYNPARNAVYARFSTDDWKKSNTANFGVSEKERAEAERLRAEAWRTVRATDQRTKARQSDASKRLNERLEDIQFWRRELQKESQLNHDESENLSEHLRVLENALQHTAKPLKISEECLMNREKRIGIDQVHDNVERQLIKEVEVIKRCQGDMQKLIDKAKVQLKLNRAALHEIDKDVKHKSHSREIDDRMYHLRDHSSGIAYHPGIETVDNSTSVPQSWARFSQENILRSQKCRAASEKLRGCIDSLLRTCANDMWSQFSTVNNALNSRVRESTDAKNKLQANLQKTMGEIFDVDRSIALLRKAIADKEAPMKVAQTRLEERTRRLDIEICNDPAMNTLQREVCEIRDSIRALKDKLRAAEAALSRLNKTKGLLENDIAVKENSIQIDAKYCLGMRKTFPMDPKVGPIFSMPMA 469
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Match: A0A267FSZ1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023660g1 PE=4 SV=1) HSP 1 Score: 531.946 bits (1369), Expect = 8.623e-171 Identity = 259/447 (57.94%), Postives = 348/447 (77.85%), Query Frame = -2
Query: 3183 SVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLA 4523
S+S P+ +R S ++ P V + + +YN ARN +Y RFS +DW+ SN A+F +SE ERA+A RL+++AW+ V+ T+ +T++RQ++A+KRLNER+++I FWR+EL +E + E ENL EHLRVL+ L+HT KPL ISE CL+ RE R GIDQV D VE+ LIKEVE IK+CQ M + + K ++Q+K+NRAA HE +KD+K KSH+ ++DD+MYHL++ SSG+AYHPGIE D + S+P SWA+FSQ+NI RSQKCRA+SE LRG ID L+R CAN MW+ FS VNN+L SRV+E T+A+NKL+++LQ+T+ EIFD ++SI LLRKAIADKE P+K+A+TRLEERT+RL++E+CNDPAM TLQ+E+ E+R+SIR LK++ R+AE +RL TK LLE DIAVKENS+QID K CLG+RK FPMD K+GPI MP+A
Sbjct: 23 SLSRALPPIAQETVGNRLSRSYTLPPGVSHAQNFYNPARNAVYARFSTDDWKKSNTANFGVSEKERAEAERLRAEAWRTVRSTDQRTKARQSDASKRLNERLEDIQFWRRELQKESQLNHNESENLSEHLRVLENALQHTAKPLKISEECLMNREKRIGIDQVHDNVERQLIKEVEVIKRCQGDMQKLIDKAKVQLKLNRAALHEIDKDVKHKSHAREIDDRMYHLRDHSSGIAYHPGIETVDKSTSVPQSWARFSQENILRSQKCRAASEKLRGCIDSLLRTCANDMWSQFSTVNNALNSRVRESTDAKNKLQANLQKTMGEIFDVDRSIALLRKAIADKEAPMKVAQTRLEERTRRLDIEICNDPAMNTLQREVCEIRDSIRALKDKLRAAEAALSRLNKTKGLLESDIAVKENSIQIDAKYCLGMRKTFPMDPKVGPIFSMPMA 469
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Match: A0A1S3HFV6 (tektin-3 OS=Lingula unguis OX=7574 GN=LOC106154939 PE=4 SV=1) HSP 1 Score: 525.013 bits (1351), Expect = 2.659e-168 Identity = 265/463 (57.24%), Postives = 344/463 (74.30%), Query Frame = -2
Query: 3183 PIVSARGR--YSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLA 4565
P+V R + + G ++P+ + S G TK V + + Y+N AR QLY RFS DW SSN+A++ +S+ ER+ A RL++DAW+AVKET+++TR+RQA+ TKRL ERV++I FW++EL E R+M+ E ENLKEH R+L++ T PL I+E CL+ RE RQGID V D+VE+SL KEV IK CQE+M + LQ+ Q+K++RAA HE EKD KDK H+H LDD+M+ L+NTS G+ YHPGIE DNTIS+P +W KF+Q+NI RSQK RA+SE LRGEID +R CAN MWN F++VNNS R++E T+ARNKL++HL RT+QE+FD EK+I LLRKAI DKE P+K+A+TRL+ER +R NVELCNDP MRTLQKE+ E+R S+R LKE+ R +EI RL+ TKS LE DI+VKENSL ID KAC+G+RK FPMD K+GPI MPLA
Sbjct: 9 PVVRTRSATPLTPDIGRIAPIGY----STGHKATKD-------VGHARTYFNPARQQLYNRFSHGDWSSSNHANYHMSDRERSFAERLRADAWRAVKETDHRTRNRQADVTKRLGERVEDIDFWKKELVNECRAMENEIENLKEHKRILEKAYMDTNNPLHIAEECLLHREKRQGIDMVHDDVERSLSKEVLIIKNCQERMRKVLQRAHAQLKLDRAALHEMEKDAKDKHHAHHLDDRMHQLRNTSMGIGYHPGIENVDNTISVPHTWVKFTQNNILRSQKERAASERLRGEIDACLRACANEMWNVFNHVNNSFNMRIRESTDARNKLQAHLNRTMQEVFDMEKNIELLRKAIHDKEAPMKVAQTRLDERCRRQNVELCNDPVMRTLQKEVNEIRESVRYLKEKLRQSEISLARLMKTKSTLEHDISVKENSLSIDSKACMGMRKGFPMDPKVGPIFNMPLA 460
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Match: K1QTB9 (Tektin-3 OS=Crassostrea gigas OX=29159 GN=CGI_10010028 PE=4 SV=1) HSP 1 Score: 506.908 bits (1304), Expect = 2.206e-161 Identity = 247/439 (56.26%), Postives = 332/439 (75.63%), Query Frame = -2
Query: 3189 FPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMP 4505
P S SR + + K YYN ARN +Y+R+S NDW SN+ +F LS+ ER+ A RL++DAW+AVKET+ +TR+RQ + TK+L ERV +I+FW+ EL+ EI +M E ENLKE+ R L++ L T PL I+E CL+ RE RQGID V D+VE++LIKEV+ IKKCQ KM + L K +Q+KMNRAAQH E D KDK H+ LDD+M L+N+SSG+ Y+PGIE DNTI+IP SW +++Q+NI RSQK RA+SE LRGEID +R CAN MWN F++VNNS +R++E T+ARNKL++HLQRT+QEIFD EK+I LLRKAI DKE P+K+A+TRL+ERT+R+NVELCNDP M++LQ+E+ E+R S+R+LKE+ +++E+ RL+ TK+ LE DI VK+N+++ID C+G+RK FPMD KIGP+ +MP
Sbjct: 28 LPYVSDKTISRHLGYAGRSLDMGHAKAYYNPARNAVYSRYSTNDWGMSNSMNFSLSDKERSFAERLRADAWRAVKETDARTRNRQNDITKKLGERVYDITFWKSELNNEISAMATEIENLKEYRRTLEKALGDTANPLHIAEECLMHREKRQGIDLVHDDVERTLIKEVDCIKKCQAKMKKVLDKAYVQLKMNRAAQHAMEIDAKDKHHAQGLDDRMQQLRNSSSGIGYYPGIESIDNTITIPDSWVRYTQENIARSQKERAASERLRGEIDSTLRACANEMWNQFNSVNNSFNTRIRETTDARNKLQAHLQRTMQEIFDMEKNIELLRKAIQDKESPMKVAQTRLDERTRRINVELCNDPVMKSLQREVTEIRESVRILKERLKASELSLARLMKTKATLEHDIGVKDNTVKIDTSYCMGMRKGFPMDPKIGPVFQMP 466
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Match: A0A0B7AIW8 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF122763 PE=4 SV=1) HSP 1 Score: 503.827 bits (1296), Expect = 3.746e-160 Identity = 240/417 (57.55%), Postives = 329/417 (78.90%), Query Frame = -2
Query: 3186 NTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPL 4436
+ K Y+N A +Y+R+ PNDW SNN+++ LSE ER+ A RL++DAW+AVKETE +TR+RQ + TKRL ERVQ+I+FW+ +L+ E+ +MD+E ENLKE+ R ++ L T PL I+E CL+ RE RQGID V D+VEKSLIKE ++IKKCQ K+ R L +Q+KMNRAAQH E D KDK H+ LDD+M L+N+S+G+ Y PGIE DNTI+IP SW +++Q+NI RSQK RA+SE +RGEID +R CAN MWNNF+ VNN+ +RV+EIT+ARNK+++HLQ+ +QEIFD E++I+LLRKAI DKE+P+K+A+TRL+ERT+R+NVELCNDP M++LQ+EI E+R S+R+LKE+ +S+E+ RL+ TK+ L+ DI+VK+NSL+ID C+G+RK FPMD K+G I +MP+
Sbjct: 52 HAKNYFNPATRTVYSRYQPNDWFMSNNSNYSLSEKERSFAERLRADAWRAVKETEARTRNRQNDVTKRLGERVQDIAFWKSQLNSEVHAMDVEMENLKEYKRTCEKALADTSNPLHITEECLMSREQRQGIDLVNDDVEKSLIKETQSIKKCQAKLKRALDNAYVQLKMNRAAQHALETDAKDKDHAQVLDDRMQQLRNSSAGVGYFPGIENIDNTITIPESWTRYTQENIARSQKERAASERIRGEIDSTLRQCANEMWNNFNAVNNAFNNRVREITDARNKMQAHLQKVLQEIFDMERNISLLRKAIHDKEKPMKVAQTRLDERTRRINVELCNDPVMKSLQREIAEIRESVRILKERLKSSELSLARLMKTKATLDHDISVKDNSLKIDSTYCMGMRKGFPMDSKVGAIFQMPI 468
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:103037718]) HSP 1 Score: 342.043 bits (876), Expect = 8.280e-104 Identity = 185/406 (45.57%), Postives = 260/406 (64.04%), Query Frame = -2
Query: 3225 VKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4442
V+ ++Y + R + TR+ P+DW SN +++ SE+ R A RL+ D + +++ + TR Q +K L ER+ +I+FWR EL EI +M E L E R L+ L T+ PL S+ CL RE R ID V D VEK LIKEVE IK CQE+M R L K Q+ NRAAQHE E+DL DK + ++DD+ + ++NTS G++Y+ GI+ D ++S+P SW+KF+ DNI SQ R++S LR EI++L+ AN MWN F++ N S +R+ E+ A+N L++HL +T+QEIF E I L+KAI DKE PLK+A+TRLEERT+R NVELC D L E+ E+ +SI L+E+ AE L+ TK LE DI+VK NSL +DQ+ C+ +R+ FP
Sbjct: 75 VRAKTMFYPSNRAAMSTRYMPDDWFKSNYNNYRESESSRNSAERLRRDTLRLIQDRDQITRWTQESTSKNLGERLNDITFWRSELHHEIDNMVSEIAALTEVKRRLERALAETEGPLQTSQECLFHREKRMSIDLVHDGVEKDLIKEVEVIKSCQERMRRSLDKAIAQLASNRAAQHELERDLNDKVTAQRIDDRCHQMRNTSDGISYYRGIDRLDPSLSLPDSWSKFTDDNILLSQSERSTSHKLRDEIEVLLNTTANEMWNQFNSANVSFTNRISEMAEAKNSLQTHLAKTLQEIFQTEMLIETLKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHHRLVGEVREIEDSIYKLRERLAEAENTLQNLVKTKVTLEHDISVKANSLFLDQEKCMSMRRSFP 480
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Match: tekt4 (tektin 4 [Source:NCBI gene;Acc:103034475]) HSP 1 Score: 179.104 bits (453), Expect = 4.698e-48 Identity = 126/389 (32.39%), Postives = 213/389 (54.76%), Query Frame = -2
Query: 3225 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4391
++SP +W+ +N+A + + + A R+ + E T+ Q+ T L R+Q++ R EL + + E E L++H R L++ L T PL IS L RE R G D VQD VE+ L+KEVE I+ Q + + LQ+ Q+++NR A+ E D DK ++ LDD+ N S+ +HP + + +W KF+Q N+ +++ S +LR D +++ A + + V+ S R E++ A+N+LE LQR + EI E++I+ L++A+ DK+ PL++A++RL R +R N+ELC D +L+ E+ ++ S+ +L++Q A +L ++ +LEK+I+ K NSL ID+ CL R +P
Sbjct: 37 KYSPREWQENNSATLQRALTDHQHAQRVLRASRALSAEAAAGTQDAQSRGTLLLGARLQDVHSRRTELQRGAERLQAETELLQDHKRRLEKALLTTDLPLTISTDNLTCRENRFGPDLVQDPVEEELLKEVELIRNVQALLKKTLQQAVDQIRLNREAKQTLELDWSDKHEAYILDDQCGRYGNRSTDTQHHPSSARLHDQVCDERAWLKFTQVNLEEAERQYRCSVSLRELCDRVLQETAEDLRAQCAVVDQSFNQRCLELSQAKNQLELQLQRVLDEIGSQERNISTLQQAVYDKDTPLRVAQSRLHYRAQRPNMELCRDQPQISLESEVEQISGSVGVLQQQLSEARQSLAQLEESRLMLEKNISCKTNSLFIDRDKCLTHRTRYP 425
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Match: tekt2 (tektin 2 [Source:NCBI gene;Acc:103026688]) HSP 1 Score: 126.716 bits (317), Expect = 2.835e-30 Identity = 100/376 (26.60%), Postives = 183/376 (48.67%), Query Frame = -2
Query: 3249 DWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKAC 4376
DWE+SN +E R + ++ + ET KT + ++++RLN+R+ +++ W++ L+ ++ ++ E + L + L T PL ++ C REGR+G + V D VE L KEV+ I Q + + + + Q+ + + A H+ DL+ K+ + +D L TS ++ P + P W +FS N+ R+++ +S LR I + N + +L R + A +++ L+ T EI + E+ I L + + K PLKL TRLE RTKR V+LC D L +E +L ++I+ LK++ A L + ++ + +D++ K+ +L ++Q+
Sbjct: 16 DWENSNKLISDTAEHRRHVSHEIRQEGRALRNETANKTSWDEYDSSRRLNDRISDVTRWKKSLEACVQDVNAEIDALTLAKEETERALAATVLPLEVTAECQTLREGRRGSELVSDPVEAELKKEVDVIDGAQRVLQQLIDQSFEQLCLLQEAHHQLTLDLQHKTEALDVDMSCLSLTVTSPEISLKPNPIRVPPGSTTPQQWEQFSHHNVIRAKEEMQTSLQLRENISITRAQVQNELEAQRIATEFALRKRTHHMEQALQEVQWQLKTTQDEITEMERDIRGLERDMQAKMAPLKLVHTRLENRTKRPGVDLCRDEVQFGLVEEAKQLDSTIQTLKQKLAQALDSLQSLQHHEAQMLEDLSRKQEALALEQRTA 391
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Match: acsf2 (acyl-CoA synthetase family member 2 [Source:NCBI gene;Acc:103037666]) HSP 1 Score: 84.3445 bits (207), Expect = 5.155e-16 Identity = 73/319 (22.88%), Postives = 151/319 (47.34%), Query Frame = 1
Query: 283 FPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEE-------------CLGKVSET-----ASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLE-----LNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNK--QLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPK--FDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKN 1158
+PD+ A++ I+ F+ +N + A+ L G+ KG G+ N+ E++++ YA + G ++ + N Y E++Y L + + C G + + +S+L ++ + + ++ PG S DLM ++G+ QL+ L+ D + I ++SGT+G PKG L+H N V++ L M ++ +++ + +P+YH +G + + GL +V P +D L A++ K ++ P + + +L P + +FD+SSV+ + + ++ ++I+ +K
Sbjct: 78 YPDREAVVFVQSGIRKTFAQFQNDVDQAAAGLVALGLKKGDRLGMWGPNTYEWVVIQYATAKAGIIMVSVNPAYQLQELEYALRKVQCKAIVCPTQFKTQKYCDMLRQLCPGMDTASPGGIKSSRLPDLSTVILTDSKQPGTFSWTDLM-EAGSSDHFNQLQDIQKTLSFDDPINIQ-FTSGTTGSPKGAALSHHNIVNNAYFIGLRMGYQWRKNVRVCVPVPLYHCFGSVG-GGIVMGLHGITMVFPSSGYDGRANLEAMQNEKCTFVYGTPTMYIDMLGQPDLAKFDISSVQTGIVAGSPCPPEVMRKVIKVMGIKE 393
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Match: acsf2 (acyl-CoA synthetase family member 2 [Source:NCBI gene;Acc:103037666]) HSP 1 Score: 84.3445 bits (207), Expect = 5.155e-16 Identity = 73/319 (22.88%), Postives = 151/319 (47.34%), Query Frame = 1
Query: 283 FPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEE-------------CLGKVSET-----ASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLE-----LNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNK--QLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPK--FDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKN 1158
+PD+ A++ I+ F+ +N + A+ L G+ KG G+ N+ E++++ YA + G ++ + N Y E++Y L + + C G + + +S+L ++ + + ++ PG S DLM ++G+ QL+ L+ D + I ++SGT+G PKG L+H N V++ L M ++ +++ + +P+YH +G + + GL +V P +D L A++ K ++ P + + +L P + +FD+SSV+ + + ++ ++I+ +K
Sbjct: 78 YPDREAVVFVQSGIRKTFAQFQNDVDQAAAGLVALGLKKGDRLGMWGPNTYEWVVIQYATAKAGIIMVSVNPAYQLQELEYALRKVQCKAIVCPTQFKTQKYCDMLRQLCPGMDTASPGGIKSSRLPDLSTVILTDSKQPGTFSWTDLM-EAGSSDHFNQLQDIQKTLSFDDPINIQ-FTSGTTGSPKGAALSHHNIVNNAYFIGLRMGYQWRKNVRVCVPVPLYHCFGSVG-GGIVMGLHGITMVFPSSGYDGRANLEAMQNEKCTFVYGTPTMYIDMLGQPDLAKFDISSVQTGIVAGSPCPPEVMRKVIKVMGIKE 393
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Sea Lamprey
Match: TEKT2 (tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725]) HSP 1 Score: 72.7886 bits (177), Expect = 2.051e-14 Identity = 48/205 (23.41%), Postives = 99/205 (48.29%), Query Frame = -2
Query: 3777 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIP 4391
RF+ +W S +++ R + ++ +A ++ +T+ Q ++ RL ER+ ++ W + L + D E L + + L+ PL ++ CL R+ R+G+D V D VE L +EV+ I++ +E + R + +Q+ + A+ + + D++DK + ++D L N S+ ++ P N + +P
Sbjct: 11 RFTVGEWHDSGERVSSGAQSRRDVSRSVRQEARHLRDQSNNQTKWDQHDSNTRLEERMSSVQRWVERLQHTLDDTDAEINALAKTKDDAERALQAKAVPLQVATECLFLRDARRGVDLVHDPVEDELHREVKAIERAKEALTRSINDAFLQVCQLQEARQQLQGDVRDKEEALEIDSTCRALNNASATISLKP------NPMRVP 209
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Sea Lamprey
Match: SLC27A5 (solute carrier family 27 member 5 [Source:HGNC Symbol;Acc:HGNC:10999]) HSP 1 Score: 73.559 bits (179), Expect = 2.633e-13 Identity = 84/351 (23.93%), Postives = 146/351 (41.60%), Query Frame = 1
Query: 124 CNFKANMTIWKIFKNGATKNHQILARSIIS----SLQPVTNVKKQTTAELIISYCSAFPDKVALI--DDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILF-ASEECLGKVSETASKLSNVK-DIYVIGNRIPG--YKSVEDLMVDSGNMSPIGQLELNLD-KDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEK 1143
C A+ W GA ++ ++L ++ S +P+ +V + ++ SA P KV L DD + DV + HA+ GV G ++ N P +L +++A +S+ C T N N + + L S L S L V E + +++ ++ +G PG S+ ++ P + ++ KD I Y+SGT+GLPK V ++H+ N M+ E D + LP+YH +I +G V++ KF ++ V V + + +LL+ PK + + SVR V D + R +E+
Sbjct: 18 CILLASWIFWPQLWLGAWRSLRVLRNAVYRHRYLSRKPLFSVLDR-----LLERASATPHKVFLTFEDDRWTYEEFVRDVARTAHALQEF---GGVRPGDTVALLMENEPAFLFVLFACTSLACPATLMNTNLRGRMLLHCLTTSKAQFLVTGSGPLLVAVEEISVEMNELQIRPLTMGEATPGSAVPSLASTFREAPCNLPAASVRAHITAKDAFIYIYTSGTTGLPKAVKVSHTRAWCGNFTYSMSGVRAE-DVVYIFLPLYHSSAIIAAFIGSLDIGARLVLRKKFSASHFWGDCRRHGVTVFQYVGEICRYLLSQPKNELDRVHSVRMAVGNGMRAD--VWARFLER 357
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005192.1 (pep scaffold:Pmarinus_7.0:GL479731:2711:5574:-1 gene:ENSPMAG00000004718.1 transcript:ENSPMAT00000005192.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.0922 bits (170), Expect = 5.013e-13 Identity = 65/266 (24.44%), Postives = 118/266 (44.36%), Query Frame = 1
Query: 124 CNFKANMTIWKIFKNGATKNHQILARSIIS----SLQPVTNVKKQTTAELIISYCSAFPDKVALI--DDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPS--ILFASEECLGKVSETASKLSNVK-DIYVIGNRIPG--YKSVEDLMVDSGNMSPIGQLELNLD-KDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYH 885
C A+ W GA ++ ++L ++ S +P+ +V + ++ SA P KV L DD + DV + HA+ GV G ++ N P +L +++A +S+ C T N N +G M + ++ + ++ S L V E + +++ ++ +G+ PG S+ ++ P + ++ KD I Y+SGT+GLPK V ++H+ N M+ E D + LP+YH
Sbjct: 17 CILLASWIFWPQLWLGAWRSLRVLRNAVYRHRYLSRKPLFSVLDR-----LLERASATPHKVFLTFEDDRWTYEEFVRDVARTAHALQEF---GGVRPGDTVALLMENEPAFLFVLFACTSLACPATLMNTNL-RGRMLLHCLTTSKAQFLVTGSGPLLVAVEEISVEMNELQIRPLTMGDATPGSPVPSLASTFREAPCNLPAASVRAHITAKDAFIYIYTSGTTGLPKAVKVSHTRAWCGNFNYSMSGVRAE-DVVYIFLPLYH 272
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Sea Lamprey
Match: TEKT2 (tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725]) HSP 1 Score: 67.781 bits (164), Expect = 1.169e-12 Identity = 57/187 (30.48%), Postives = 92/187 (49.20%), Query Frame = -2
Query: 3234 SIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLL---EKDIAVKENSLQIDQKACLGVRK 3785
S P +W +FS+ N+ R+ +S ALR I I N + ++ + + R E+ + +LE + QEI E I L + K PLK+A TRLE RT R VELC D + E+ ++ +I LK++ + YN L + L E+D+A K +L ++++ C+ VR+
Sbjct: 2 SSPRTWDEFSRRNVERAMAEMRASSALREAITATIAQTTNELEAQKNSTDFAFRKRSHELDKVKAELEWQKKNNKQEISVLEGDIAHLEADVRAKMLPLKVAHTRLETRTYRSGVELCRDEPQYGITTEVHQIEATIATLKKKLSDS---YNALTGLRCSLERVERDLASKALALGLERR-CVDVRR 184
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Match: EDO39292 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAK1]) HSP 1 Score: 224.172 bits (570), Expect = 2.613e-64 Identity = 143/388 (36.86%), Postives = 232/388 (59.79%), Query Frame = -2
Query: 3234 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRV-LKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAY-HPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRK 4391
++ P +W +SN+ + +E ERA A RL+ ++ + E E +T Q + K+L++R+ +I+FW+ ELD+ +S E+ N+ + + L+ L T+ PL I++ CL+ RE R ID V D VE L+KEVE I+ Q + R +++ Q+++ R+A++ EKD KDK H++++D+ LKN S GL Y +++ N+++ P W FS N+ ++++ + SS +LR ID L+ N + N VN + R++E A+ KLE HL + +EI E++I L++AIA K P+K+A+TR+E RT R N+ELC D L E++E+ +I L+E+ AE LI + LE+DI VKENSL ID+ C+ +RK
Sbjct: 10 KYVPEEWHASNHLQYFTAEKERAAAERLRDESERLRVEIEVRTVETQEDVNKKLDQRITDINFWKSELDRN-QSETEEEINMMLNFKARLERALADTELPLMIAQQCLMNREKRIKIDLVHDNVEMQLLKEVEVIEGVQALLKRTIEQATEQIRLLRSARYYLEKDRKDKFHANKIDNTCAALKNDSPGLVYARDAVKIEKNSVT-PDEWEDFSNKNMLKAEREKNSSTSLRSIIDGLLVQTLNDLMNQCRAVNLAFEKRIEETLKAKTKLEDHLMKVKEEIAAMEENIATLQRAIALKIAPMKVAQTRVENRTFRPNIELCRDRVQYRLIDEVVEISTNIERLQEKLYEAESSLKALIRRQLSLEEDIEVKENSLFIDRDQCMELRK 395
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Match: EDO40681 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6M2]) HSP 1 Score: 194.512 bits (493), Expect = 3.055e-54 Identity = 131/369 (35.50%), Postives = 200/369 (54.20%), Query Frame = -2
Query: 3234 SEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRK 4340
E ERA A RL+ + + ET T Q + K+L+ R+ +I++W+ EL+++ E + L+ + L++ L T+KPL IS+ CL RE R ID V D VE L KEVE I+ Q + + + + Q+ + R+A+ + EKDL DK + +D K L N S + + P P W +S +NI ++++ R +S ALR EI+ ++ + N + VNN R+++ A+ LE L R + E+ + +I L +AIADKE PLK+A TRL++R+ R NVELC DP L E+ E+ SI L+ + A+ L+ K LE DI VK NSL ID+ C+ VR+
Sbjct: 7 GEKERATAERLRDECSRLRTETSLLTHRTQMDVNKKLDHRLHDINYWKAELEKQHSETVAEIKALQAFIGRLEKALAATEKPLNISQQCLGYRERRVKIDLVHDNVETHLSKEVEMIENVQALLKKTMDQAHEQLWLLRSAKTQLEKDLGDKYGALGIDGKCSTLNNHSKDIHFAPHAVTIQQHSFTPDEWEAYSSENIAKAERERKASVALRSEINGILMQTYVDLRNIYETVNNEFHKRIEDTVGAKKSLEKELSRVMDELASMDNNIRTLTQAIADKEAPLKVAHTRLDKRSIRPNVELCRDPVQYRLVGEVGEINTSIDRLRMRLAEAQASMQALLRNKENLEDDIEVKTNSLFIDRDQCMVVRQ 375
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Match: EDO37357 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG13]) HSP 1 Score: 164.851 bits (416), Expect = 9.801e-44 Identity = 121/386 (31.35%), Postives = 199/386 (51.55%), Query Frame = -2
Query: 3234 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRK 4391
RF+ +DW +SN +E +R + R++ ++ ET+ TR Q ++ +L +R+ +I+ W++ L++ + D E L + LE + PL I+ CL+ RE RQ ID V+DEVE L KEVE I+ + + + + + Q+ + + A+H+ D+ DK + Q+D Y + N S+ + Y P +W FS N R++ +S+ LR I ++ AN + S R+ E A+++LE + T++EI E I L++AI K P+ +A+TRLE RT R NVELC D L E+ E+ S+R L E+ R A+ L + S + +D+A K NSL +D + C+ VR+
Sbjct: 11 RFAVSDWHTSNQIIRTNAERQREASHRVRQESRFLRNETDNHTRWTQHDSNTKLEKRIDDINDWKRSLERCLAETDNEIALLTREKERTERALEAKKVPLDITLECLMLRENRQSIDLVRDEVEAQLHKEVEVIEGTKALLQQKVDESFEQLCLLQEARHQLHLDITDKFTTLQIDGDCYAMNNMSTNIGYQVNPTRTVKGSVTPETWDSFSNYNKLRAEAEMKASKHLREAIFATLQQTANDLEAQRKASEYSFRKRIHETDQAKSELEWQQKNTMKEIATLEDDIKGLKEAIDAKNAPMMVAQTRLENRTYRPNVELCRDQPQYQLCHEVAEIAGSMRALTEKLREAQNSLEALRSNLSRINEDLAFKNNSLALDNR-CMSVRE 395
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Match: EDO46392 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPW4]) HSP 1 Score: 166.777 bits (421), Expect = 2.423e-43 Identity = 109/310 (35.16%), Postives = 175/310 (56.45%), Query Frame = 1
Query: 277 SAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVED-LMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSS----NKQLEMTHESKESDS---YMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQG 1182
+ + D+ ALID A + FS++ I S L + G G +I N EY ++ Y S+G VT N YT EM QL S + + + E + +V++ A+K S V+ ++V+ + PG++++ D ++ D G+ P + +N +D+A + YSSGT+GLPKGV+LTH N +S+ N MT E S +LI+PM+HV+G+ + +G+ V +FDPV L AI+KYKV + ++PPL++FL HP V ++DLSSV+ CGAA L +++ + + +F P+ + QG
Sbjct: 29 AEYGDEKALIDSATGKSFTFSELCTLIRKCGSVLVRRGAQIGDTMAVILPNMIEYPVVCYGALSVGMRVTTLNPQYTVREMVPQLKDSQANYIITTPELIHQVNQAAAKCSCVRRVFVLAD-TPGHQTLYDQILNDDGSAFP-SHVPVNWKQDVAYILYSSGTTGLPKGVLLTHYNLISAVVILNNFWAMTSEQTTEASKIIQVLIVPMFHVFGLAIMLGINIAIGVTMVCIRQFDPVSFLEAIQKYKVTNISVVPPLLIFLAKHPSVLKYDLSSVKSVGCGAAPLGEEMMDAFMSRF----PNVESNQG 332
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Match: EDO46794 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RP51]) HSP 1 Score: 145.976 bits (367), Expect = 1.925e-36 Identity = 95/297 (31.99%), Postives = 157/297 (52.86%), Query Frame = 1
Query: 262 IISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLM-VDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYML-ILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKF 1146
++ + ++ ++ A++D Y ++ + I SAL + G+ + ++ N PEY ++ Y ++S+G +VT N YT E+ YQL S L + K + A + + VK IY G + G KS+ DL+ D G P+ + +N +D+ LPYSSGT+GLPKGVMLTH N + D L +LPM+H YG+ + G + +F+P L AI+K+K+ +L ++PP+VLFL HP V++++L S+++ GAA L + N L+ +
Sbjct: 24 VLKHMESYGEETAMVDSTSGRSYTYNKLTEMIKKCGSALIRQGLQTKDMVAVLLPNMPEYPIVFYGVTSVGGIVTTINPAYTTDEIIYQLKDSGAKYLVTVPQLAQKAKQAADQ-AGVKRIYTFG-YVDGCKSLFDLLHEDDGGSYPV-SVSVNWKEDVVCLPYSSGTTGLPKGVMLTHYNLIHHAAMFSNDDVMSSEDLITLGLLPMFHSYGLSILMGVCLIKGASVICMTQFEPTHFLEAIQKFKITMLPVVPPIVLFLAKHPLVEKYNLMSIKQVTSGAAPLGAEQINALMTRM 317
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:101160513]) HSP 1 Score: 338.576 bits (867), Expect = 1.311e-102 Identity = 193/487 (39.63%), Postives = 293/487 (60.16%), Query Frame = -2
Query: 3207 TLAVNFIQPICLWYIPMASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKI--------YYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP-MDQKIG 4640
TL N+ +P ++P S +++ R + +P R+ N F ++ S + P +++ + ++ + R + R++P+DW SN +H+ SE+ R A RL+ D + +++ + TR Q +K ++ER+ +I FW+ EL EI +M E L E R L+ L TQ PL +++ CL RE R ID V D VEK LIK +E IK CQE+M L + Q+ NRAAQHE EKDL DK + ++DD+ +HL+NTS G+ Y+ GI+ D+ +S+P SW++F+ DNI RSQ RA+S LR EI++L+ +N MWN F+NVN + +R+ E T+A++ L++HL +T+QEIF E I L+KAI DKE PLK+A+TRLEERT+R N+ELC D L E+ E+ ++I+ L E+ AE L+ TK LE D+++K NSL +DQ+ C+ +RK FP M + +G
Sbjct: 7 TLTANYARPKTSHFLPAIST-------MASSYRNPQQVMAFNPSANIWRT-NSFYKSNSIMPSTPSIQHDGLDLVRARTSFFPSNRTAMTNRYTPDDWYKSNQSHYWESESSRKSAERLRKDTMRLIQDKDQLTRRTQENTSKNISERLNDIRFWKSELSHEIDNMVTEIAALTEVKRRLERALAETQGPLQVAQECLYHREKRMSIDLVHDGVEKDLIK-IEVIKSCQERMRLHLDRATSQLASNRAAQHELEKDLSDKLTAQRIDDRCHHLRNTSDGIGYYRGIDRQDHAVSLPDSWSRFTDDNILRSQSERATSHKLRDEIEILLNTTSNEMWNQFNNVNVAFTNRISETTDAKSSLQAHLAKTMQEIFQTEMLIESLKKAIRDKENPLKVAQTRLEERTRRPNIELCRDNPYHRLVTEVREIEDTIQKLHERLMEAENTLQALVKTKVKLENDLSIKANSLFLDQEKCMSMRKTFPSMPRLVG 484
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Match: tekt4 (tektin 4 [Source:NCBI gene;Acc:101173527]) HSP 1 Score: 181.415 bits (459), Expect = 1.199e-48 Identity = 124/390 (31.79%), Postives = 206/390 (52.82%), Query Frame = -2
Query: 3225 TRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFP 4394
T++SP +W S+ + A+ +A ++ + +++E T QAE T+ L ER+Q I WR EL + I + + E L+ L+ LE T P AI+ L R R G D VQD VE+ L+KEV I+ Q + R + + Q++ N A+ E D DK ++ LD++ N S HP + P +W KF+QDN+ ++ + +S LR ++ ++R + N S V+ + R QE+ A+ +L+ L + +Q+I + EK+I L+KAI KE PL++ ++RL R+ R N+ELC DPA +L+ E ++ ++ LK++ A I + L ++ L+KDI+ K SL I+ + C+ R+ P
Sbjct: 47 TKYSPAEWFSNYQTILVRAGADNLEAESIQQLSRTLNRDSEGVTLQTQAEGTRLLGERLQEIHHWRSELLRHIEQLQADMEALQAARTRLERALEATDTPYAIATDNLNCRAKRPGPDLVQDSVEEELLKEVGLIRNIQALLKRTIAQVASQIRRNMDAKQVLEMDWSDKQEAYNLDERGGGHSNRSPDTQLHPSSAANHEQVCNPTTWTKFTQDNLLQAAQEEEASRRLRALVEEMLRETTEDLINQRSTVDGAFSQRCQELLEAKTELQIKLTQVLQQIGEQEKNIVGLQKAIHLKEAPLRVVQSRLHLRSLRPNMELCRDPAQLSLEAEAAQIETTLNSLKQKLNEARISLSNLEESRMELQKDISCKSQSLFIEGEKCMTQRRHLP 436
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Match: tekt2 (tektin 2 [Source:NCBI gene;Acc:101164907]) HSP 1 Score: 124.02 bits (310), Expect = 2.013e-29 Identity = 96/380 (25.26%), Postives = 187/380 (49.21%), Query Frame = -2
Query: 3252 RFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKA 4391
R ++W ++N + ++ +R ++ ++ + ET KT + E ++RL ERV ++S WR LD + +D E + L + ++ L T PL +S C++ REGR+G + V D V++ L KEVE I++ Q+ + + + + Q+ + + A+H+ DL++K + +D L TS ++ + S P W + S N+ ++++ S+ +R ++ + N + +L R AR++L+ ++ EI + E I L + K PLKLA TRLE RT R ++LC D +L E+ +L ++ L+ + A+ + + +D++ K+ +L ++Q++
Sbjct: 9 RCGVSEWNNNNLQLWAEAQQQRLVSSEIRQECKSLRNETHCKTVWDEKETSRRLTERVWDVSRWRDLLDSCAQKVDEEMDALTLSKELTEQALAATATPLEVSTECMMLREGRRGYELVTDPVQEQLQKEVELIERVQQTLQQHIDEAFQQLCVLQEARHQLTGDLQNKMEALDIDKSCLTLTITSPNISLKTNPTRIPSGASTPQEWVQLSNHNVVQAEEAMRVSQQMREDMSLSRAQLQNELEAERRATEFALRKRSHHEEQARDELQWQIRNMEAEIAEMESDIRELDADLQAKTAPLKLAHTRLENRTHRPGMDLCRDEVQYSLVAEVEQLEATVLALQLKLSKAQHSLQNMRLHHGQMLQDLSRKQEALSLEQRS 388
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Match: tekt1 (tektin-1 [Source:NCBI gene;Acc:101161072]) HSP 1 Score: 120.939 bits (302), Expect = 1.108e-28 Identity = 93/364 (25.55%), Postives = 191/364 (52.47%), Query Frame = -2
Query: 3237 QATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPV--SWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVR 4322
Q+ R +++ + +KET+ + Q + ++ +++++ I F ++EL+ ++ +++E ++L + + L+ ++PL +++ C+ +R R ++ DEV+ L+KE E +K+ + + R ++ Q+++NR+A++ EKDLK+KS + +D+ + S + P S+ W S N+ ++++ + +S +L+ + ++ A M ++ VQ++ NA+N++E L + EI K+ LR A+A+ EE L L + RL R +R E DPA R L ++ ++ I+ L+++ +E RL+N K L++ I K N L ID+ C+ R
Sbjct: 22 QSERFRAECKELIKETKQICKRMQEDENQQFDQKLRRIQFLKKELELKLDEVNLEIDDLVVLQSRVTKALDACKEPLEVTDLCMKERLKRPNSERFHDEVDGELLKESEGLKRVESLLQRVTEQISEQLRLNRSAKYGIEKDLKEKSEAQNVDNFCTLMATLSMSHRHRPKTHSTPEPSSVLTLDQWENISYANMAKAEQQKTNSRSLQVLVQSVLDQTAADMQKQVQATTTAIQLNVQKMKNAKNQMEDELVKIQSEISSQRKTREDLRVAVAENEEFLSLTQARLALRHERPGKEQWRDPAQRKLLADVQKITEHIKQLQQEVDRSEDEERRLLNWKLRLQESIDSKANCLYIDEVVCMQRR 385
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Match: slc27a2a (very long-chain acyl-CoA synthetase [Source:NCBI gene;Acc:101155585]) HSP 1 Score: 74.7146 bits (182), Expect = 4.888e-13 Identity = 78/304 (25.66%), Postives = 137/304 (45.07%), Query Frame = 1
Query: 286 PDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGK-GTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKY-------QLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIP--GYKSVEDLMVDSGNMSPIGQLELNLD-KDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGL------GLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKF 1146
PDK I + + YK +D +++ +A AL+ + K G + N P + + AL+ +GCV N+N + + +++ ++P + A EE L + E + + V+G+ + G++S+ + ++ + +L N+ K A+ Y+SGT+GLPK ++TH S++ EM + D + L LP+YH G FL GL G+ V++ KF KY + V+ I ++ +L N PK D +VR D E +E+F
Sbjct: 63 PDKKLFIFEDVSYTYKQADAESN--KVARALSAHAQLKPGDTVALFLGNEPLLVWVWLALAKLGCVTALLNYNIRSRSLLHCFSCCGAKILITSPDLREAVEEVLPTLREQGIR------VLVLGDHLEADGFESLFHKVQEASDQPLSPELRANIHHKSPALYIYTSGTTGLPKAAIITHQRAWSASLAQEMVG-VRSDDIFYLYLPLYHTAG------FLMGLCGGINKGVTFVLKRKFSVSSFWDDCRKYNITVIQYIGEIMRYLCNMPKKDNDKDHNVRLAFGNGIRTDTWAE--FLERF 349
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Match: SMESG000064101.1 (SMESG000064101.1) HSP 1 Score: 940.258 bits (2429), Expect = 0.000e+0 Identity = 469/471 (99.58%), Postives = 471/471 (100.00%), Query Frame = -2
Query: 3180 MASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQVKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLAY 4592
MASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQ+KNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETE+KTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWNNFSNVNNSLLSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLAY
Sbjct: 1 MASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQIKNTKIYYNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEHKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWNNFSNVNNSLLSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIGPILEMPLAY 471
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Match: SMESG000041780.1 (SMESG000041780.1) HSP 1 Score: 679.478 bits (1752), Expect = 0.000e+0 Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 1
Query: 142 MTIWKIFKNGATKNHQILARSIISSLQPVTNVKKQTTAELIISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQG 1182
MTIWKIFKNGATKNHQILARSIISSLQPVTNVKKQTTAELIISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAVLHLIPPLVLFLLNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQG
Sbjct: 1 MTIWKIFKNGATKNHQILARSIISSLQPVTNVKKQTTAELIISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAVLHLIPPLVLFLLNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQG 347
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Match: SMESG000041777.1 (SMESG000041777.1) HSP 1 Score: 324.709 bits (831), Expect = 8.189e-100 Identity = 167/329 (50.76%), Postives = 234/329 (71.12%), Query Frame = 1
Query: 196 ARSIISSLQPVTNVKKQTTAELIISYCSAFPDKVALIDDARNIKYKFSDVKNSIHAIASALTKNGVGKGTIAGIIASNSPEYLLLIYALSSIGCVVTACNHNYTQGEMKYQLMHSNPSILFASEECLGKVSETASKLSNVKDIYVIGNRIPGYKSVEDLMVDSGNMSPIGQLELNLDKDLAILPYSSGTSGLPKGVMLTHSNFVSSNKQLEMTHESKESDSYMLILPMYHVYGMICIPNFLFGLGLPAVVQPKFDPVKMLTAIEKYKVAXXXXXXXXXXXXXNHPKVDQFDLSSVRKTVCGAASLDQQLENRLIEKFKVKNPDFFTTQG 1182
AR+II+S + KK+T AE + ++C + + +A IDD R Y +SDVK + +IASALTK GV +GT+AGII++NSPEY++LIYALSS+GC VT CN N+T E+ QL S PSI+F ++ECL KV+ S+LS VK+IY+IG+ +PGY++V+ LM DSGNM+PIGQ+ L+LD +A++PYSSGT+GLPKGVMLTH+N V++ +Q+ HE + ++ LP+YH+Y + I + F G V Q KF+P L AIE+YK++VLHL+PP +LFLLN+ + F+L+S+ K AA LD +ENR IE F+ P+F T QG
Sbjct: 19 ARTIITSKYENISYKKETIAEHVFTFCDKYSNNIAFIDDVRKTSYTYSDVKGLVSSIASALTKRGVKQGTMAGIISTNSPEYVMLIYALSSMGCSVTICNQNFTSREIINQLSDSQPSIIFTNKECLSKVTNVISELSTVKNIYLIGDPLPGYETVDKLMADSGNMNPIGQINLDLDSHIALMPYSSGTTGLPKGVMLTHNNLVANFRQINKAHEISDVKKMLIALPLYHIYSQVIIISMGFFSGSQMVFQSKFEPTNYLRAIEQYKISVLHLVPPQILFLLNNHETKNFNLTSIEKCFSAAAPLDAVVENRFIETFREHQPNFDTFQG 347
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Match: SMESG000021346.1 (SMESG000021346.1) HSP 1 Score: 325.865 bits (834), Expect = 3.890e-98 Identity = 167/405 (41.23%), Postives = 260/405 (64.20%), Query Frame = -2
Query: 3207 YNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIG 4421
++ ARN LYTR++P DW ++ F+ ++ R R++ D + +ET+ +T+ Q + K++ +R+ + +FW+ ELD EI M E NL E RV ++ L T+ L I E CL RE RQGID V D VEKSLI E++T+K+ Q+ + + K QM +NRAAQHE E D +DK + Q+D+ + +KN+S+G+ +H G+E DN +S P +WAK+S DN+ RSQ R +S +R EI+ + AN MW ++NVN +L R+QE +ARNK+++HL + +QEIFD EK+I L+K I DKE+ +K+A+TRL R +R +ELC D L +E+ E+R + +L + R AE L++ K++LE+D+ +K N+L +D++ CLG+RK FP+ ++
Sbjct: 91 FSAARNALYTRYTPKDWMTAAMKTFQSADRVRDYGERIRFDTNRLGRETDDQTKRAQEDVGKKIGDRLSDTNFWKTELDDEIDKMITENNNLNEMKRVAEKMLGETENQLHIGEECLYTREKRQGIDLVHDNVEKSLIGEIDTVKQAQDDIRNIISKANAQMSLNRAAQHENELDSRDKFIAQQIDENAHQIKNSSAGINFHNGVETIDNCVSTPETWAKYSDDNVKRSQAERENSTKVREEIEATLDKAANDMWKRWNNVNTALTERIQETIDARNKIQAHLAKILQEIFDMEKNIEYLKKCIKDKEDYMKVAQTRLHFRLRRPGMELCRDQPNHRLVEEVDEIREVVAMLMRELREAETALQHLLDNKAVLEQDLNIKNNTLFLDREKCLGLRKTFPIQPRLA 495
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Match: SMESG000021346.1 (SMESG000021346.1) HSP 1 Score: 325.865 bits (834), Expect = 5.876e-98 Identity = 167/405 (41.23%), Postives = 260/405 (64.20%), Query Frame = -2
Query: 3207 YNTARNQLYTRFSPNDWESSNNAHFKLSEAERAQATRLKSDAWKAVKETEYKTRSRQAEATKRLNERVQNISFWRQELDQEIRSMDIEQENLKEHLRVLKENLEHTQKPLAISEHCLVKREGRQGIDQVQDEVEKSLIKEVETIKKCQEKMNRFLQKGEIQMKMNRAAQHECEKDLKDKSHSHQLDDKMYHLKNTSSGLAYHPGIELFDNTISIPVSWAKFSQDNINRSQKCRASSEALRGEIDMLIRNCANIMWXXXXXXXXXXXSRVQEITNARNKLESHLQRTVQEIFDCEKSINLLRKAIADKEEPLKLAETRLEERTKRLNVELCNDPAMRTLQKEIMELRNSIRLLKEQKRSAEIVYNRLINTKSLLEKDIAVKENSLQIDQKACLGVRKIFPMDQKIG 4421
++ ARN LYTR++P DW ++ F+ ++ R R++ D + +ET+ +T+ Q + K++ +R+ + +FW+ ELD EI M E NL E RV ++ L T+ L I E CL RE RQGID V D VEKSLI E++T+K+ Q+ + + K QM +NRAAQHE E D +DK + Q+D+ + +KN+S+G+ +H G+E DN +S P +WAK+S DN+ RSQ R +S +R EI+ + AN MW ++NVN +L R+QE +ARNK+++HL + +QEIFD EK+I L+K I DKE+ +K+A+TRL R +R +ELC D L +E+ E+R + +L + R AE L++ K++LE+D+ +K N+L +D++ CLG+RK FP+ ++
Sbjct: 103 FSAARNALYTRYTPKDWMTAAMKTFQSADRVRDYGERIRFDTNRLGRETDDQTKRAQEDVGKKIGDRLSDTNFWKTELDDEIDKMITENNNLNEMKRVAEKMLGETENQLHIGEECLYTREKRQGIDLVHDNVEKSLIGEIDTVKQAQDDIRNIISKANAQMSLNRAAQHENELDSRDKFIAQQIDENAHQIKNSSAGINFHNGVETIDNCVSTPETWAKYSDDNVKRSQAERENSTKVREEIEATLDKAANDMWKRWNNVNTALTERIQETIDARNKIQAHLAKILQEIFDMEKNIEYLKKCIKDKEDYMKVAQTRLHFRLRRPGMELCRDQPNHRLVEEVDEIREVVAMLMRELREAETALQHLLDNKAVLEQDLNIKNNTLFLDREKCLGLRKTFPIQPRLA 507 The following BLAST results are available for this feature:
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of 4-coumarate--CoA ligase 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30005068 ID=SMED30005068|Name=4-coumarate--CoA ligase 2|organism=Schmidtea mediterranea sexual|type=transcript|length=4752bpback to top protein sequence of SMED30005068-orf-1 >SMED30005068-orf-1 ID=SMED30005068-orf-1|Name=SMED30005068-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MPIHIALLDIGCTNSKVVLSQCLARKRTVDQLKFKKNTEKLVEIVQEEHRback to top protein sequence of SMED30005068-orf-2 >SMED30005068-orf-2 ID=SMED30005068-orf-2|Name=SMED30005068-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=472bp MASISSINRPIVSARGRYSNNDGSVSPVRFPLRSSNGFSRTKSSISVFPQback to top protein sequence of SMED30005068-orf-3 >SMED30005068-orf-3 ID=SMED30005068-orf-3|Name=SMED30005068-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=388bp MTIWKIFKNGATKNHQILARSIISSLQPVTNVKKQTTAELIISYCSAFPDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
