Lysine-specific demethylase 6A

Overview


Name	Lysine-specific demethylase 6A
Smed ID	SMED30005027
Length (bp)	2110

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Lysine-specific demethylase 6A (SMED30005027) t-SNE clustered cells

Violin plots show distribution of expression levels for Lysine-specific demethylase 6A (SMED30005027) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Lysine-specific demethylase 6A (SMED30005027) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Lysine-specific demethylase 6A (SMED30005027) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30005027

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
X2 cell	SMED30005027	SMESG000030162.1	SmedASXL_014184	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
protonephridia	SMED30005027	SMESG000030162.1	dd_Smed_v4_12343_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30005027	SMESG000030162.1	dd_Smed_v4_12343_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30005027	SMESG000030162.1	dd_Smed_v4_12343_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30005027	SMESG000030162.1	dd_Smed_v6_12343_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Match: A0A1I8HYR5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.258e-17
Identity = 77/305 (25.25%), Postives = 143/305 (46.89%), Query Frame = 2

Query:   68 SHVFIKMYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIRSNFKFEKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSI--EFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQ 976
            S ++  M       + +W+IWY  L+     ++ YS+ +RY  F+++    +    W M+P++ Y+  +I ++ L  FF+Y  +    +     ++     ++D  +      S   ++L R  +PY  +LH++ A CLL+P+PV+E  +I +    SD IW  N DF                     I++   S+T S+  E  N ++A  IL++++PS  W+SN+ F+  FS I+    L  ++ YE  SV  K S + G       L   I      +  ++  L +++  +  F++G  Q
Sbjct:    2 SELYQAMAKTRAKFDKMWRIWYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQ--IGRDRDCLRVAIAETSSGAAALRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDF---------------------IYHPKASATNSVCPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNGVVTQVNLLPPAI-----IALHSIGDLLLLVANIAFFEFGYAQ 274

[Close]

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Match: A0A267H7U1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g1 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.288e-15
Identity = 77/299 (25.75%), Postives = 137/299 (45.82%), Query Frame = 2

Query:  110 NNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPN-----GNIRSNFKF---EKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSI--EFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQ 976
            + +W+IWY  L+     ++ YS+ +RY  F+++    +    W M+P++ Y+  +I ++ L  FF+Y  +    +        G  R   +    E          +   +S L R  +PY  +LH++ A CLL+P+PV+E  +I +    SD IW  N DF                     I++   S+T S+  E  N ++A  IL++++PS  W+SN+ F+  FS I+    L  ++ YE  SV  K S + G       L   I      +  ++  L +++  +  F++G  Q
Sbjct:    9 DKMWRIWYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMIITVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDF---------------------IYHPKASATNSVCPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNGVVTQVNLLPPAI-----IALHSIGDLLLLVANIAFFEFGYAQ 277

[Close]

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Match: A0A267E669 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g3 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.422e-15
Identity = 77/299 (25.75%), Postives = 137/299 (45.82%), Query Frame = 2

Query:  110 NNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPN-----GNIRSNFKF---EKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSI--EFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQ 976
            + +W+IWY  L+     ++ YS+ +RY  F+++    +    W M+P++ Y+  +I ++ L  FF+Y  +    +        G  R   +    E          +   +S L R  +PY  +LH++ A CLL+P+PV+E  +I +    SD IW  N DF                     I++   S+T S+  E  N ++A  IL++++PS  W+SN+ F+  FS I+    L  ++ YE  SV  K S + G       L   I      +  ++  L +++  +  F++G  Q
Sbjct:   42 DKMWRIWYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDF---------------------IYHPKASATNSVCPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKASMSNGVVTQVNLLPPAI-----IALHSIGDLLLLVANIAFFEFGYAQ 310

[Close]

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Match: A0A267GLJ3 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030458g2 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.560e-15
Identity = 72/267 (26.97%), Postives = 124/267 (46.44%), Query Frame = 2

Query:   68 SHVFIKMYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPN-----GNIRSNFKF---EKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSI--EFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKIS 838
            S ++  M       + +W+IWY  L+     ++ YS+ +RY  F+++    +    W M+P++ Y+  +I ++ L  FF+Y  +    +        G  R   +    E          +   +S L R  +PY  +LH++ A CLL+P+PV+E  +I +    SD IW  N DF                     I++   S+T S+  E  N ++A  IL++++PS  W+SN+ F+  FS I+    L  ++ YE  SV  K S
Sbjct:   28 SEIYQAMAKTRAKFDKMWRIWYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDF---------------------IYHPKASATNSVCPEVFNLMLALAILLVRYPSTFWFSNRAFAFMFSVILCVCGLHIVLSYEATSVLFKAS 269

[Close]

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Match: A0A1I8IPE7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.529e-14
Identity = 59/222 (26.58%), Postives = 105/222 (47.30%), Query Frame = 2

Query:  110 NNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPN-----GNIRSNFKF---EKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSI--EFINFIVAAVILVIQFPSGLWYSNK 745
            + +W+IWY  L+     ++ YS+ +RY  F+++    +    W M+P++ Y+  +I ++ L  FF+Y  +    +        G  R   +    E          +   +S L R  +PY  +LH++ A CLL+P+PV+E  +I +    SD IW  N DF                     I++   S+T S+  E  N ++A  IL++++PS  W+SN+
Sbjct:    9 DKMWRIWYAILVLALNAFLLYSAFQRYASFRAKALREQ----WDMSPMNFYLAMMILTVFLLPFFLYAALAKTANYAEDGVQIGRDRDCLRVAIAETSSGAGAQATVGTCMSRLRRHFMPYTCLLHVLTAFCLLIPMPVLEAAEIHHAKRPSDEIWRTNLDF---------------------IYHPKASATNSVCPEVFNLMLALAILLVRYPSTFWFSNR 205

[Close]

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Match: SMESG000030162.1 (SMESG000030162.1)

HSP 1 Score: 1049.27 bits (2712), Expect = 0.000e+0
Identity = 610/614 (99.35%), Postives = 612/614 (99.67%), Query Frame = 2

Query:   86 MYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIRSNFKFEKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQSAISCLKGLKTFSKSYWLCLVIVVGFGLVFSTLIIELLMFIVFFQCFQMFHXXXXXXXXXXXXXXXXYQILAWFTFSIKNRWVFQLDLPELTQGSSCNTDGNNKYTGSPDTIYGCFHNNITLXXXXXXXXERGMGRIIETPSAENGCYGTNYQNYVYLQNPTALIPTISEDYDENXXXXXXXXXINRKETRNNSLTDQDLIYRTASSISDIGLIKMKENLNSDSYISLQMNNSRKAGKLVTFHEIGEEKIPFFGSYNDQYSSNNMEQLDFLKVPDLNNLNRXXXXXXXXXXXXXXXXXXXXXXXXXXXNENGLCSEV 1927
            MYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCII SISLFLFFIYFCVISVRSSPNGNIRSNFKFEKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCFSIFFSSIIFFNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQSAISCLKGLKTFSKSYWLCLVIVVGFGLVFSTLIIELLMFIVFFQCFQMFHIPIFLISIV+GIINIIYQILAWFTFSIKNRWVFQLDLPELTQGSSCNTDGNNKYTGSPDTIYGCFHNNITLSTSSSHSSERGMGRIIETPSAENGCYGTNYQNYVYLQNPTALIPTISEDYDENTTSTSKSSSINRKETRNNSLTDQDLIYRTASSISDIGLIKMK+NLNSDSYISLQMNNSRKAGKLVTFHEIGEEKIPFFGSYNDQYSSNNMEQLDFLKVPDLNNLNRSTLSGSQSESSLDQISSSFS KQAIVSNENGLCSEV
Sbjct:    1 MYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCIITSISLFLFFIYFCVISVRSSPNGNIRSNFKFEKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCFSIFFSSIIFFNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQSAISCLKGLKTFSKSYWLCLVIVVGFGLVFSTLIIELLMFIVFFQCFQMFHIPIFLISIVIGIINIIYQILAWFTFSIKNRWVFQLDLPELTQGSSCNTDGNNKYTGSPDTIYGCFHNNITLSTSSSHSSERGMGRIIETPSAENGCYGTNYQNYVYLQNPTALIPTISEDYDENTTSTSKSSSINRKETRNNSLTDQDLIYRTASSISDIGLIKMKDNLNSDSYISLQMNNSRKAGKLVTFHEIGEEKIPFFGSYNDQYSSNNMEQLDFLKVPDLNNLNRSTLSGSQSESSLDQISSSFSPKQAIVSNENGLCSEV 614

[Close]

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Match: SMESG000077784.1 (SMESG000077784.1)

HSP 1 Score: 126.331 bits (316), Expect = 5.591e-30
Identity = 133/439 (30.30%), Postives = 211/439 (48.06%), Query Frame = 2

Query:  101 WNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIRSNFKFEKDGQKNCFGPISR------LVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFF--SDQFRWLTMESLGDGNPYK-NIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQSAISCLKGLKTFSKS--------------YWLCLVIVVGFGLVFSTLIIELLMFIVFFQC--FQMFHXXXXXXXXXXXXXXXXYQILA----WFTFSIKNRWVFQLDLPELTQGSSCNTDGNNKYTGSPDTIYGCFHN 1330
            +NC+ LWK+WY  LI V Q+ +   +I  Y+ +KSQ ++PR+GN W   PL+  +  I+ S+SL ++ IY C+  V    N  I      + D  K     IS        ++ L+   LPY   +HL +A  L LP+ +++ EQI+NEA++ D IW  N DF      FR     ++     YK    Y  K S ++ EFIN I++ +I+VI+ PS  WY+NK FSI FS   F      L+ YE+  + IKI   I ++++  K+   +    +     +  L MI   + IF+YG TQ   +  K  + FS                Y    V    FG++   ++  L + I++ Q    ++         I++ I   ++  L+    WF   +KN+W F +++    Q +     G  K   SP+ IYGCF+N
Sbjct:    9 FNCDRLWKLWYSILIIVMQVLLISLAISNYLTYKSQSFDPRFGNHWSALPLNFQLASIVLSLSLLVWLIYSCMFKVGHIANDGIV--LGLDLDSIKKALEEISSYKGIKLFLTRLITHVLPYNCFIHLSIALLLCLPVALMQAEQIKNEAISPDYIWTTNIDFLISPHSFRIFKANTVIC---YKLPCEYPTKPSAIAPEFINLIISLLIIVIRLPSPFWYTNKAFSILFSIFAFITVSCILMEYESLILIIKI---IHNYDLDTKIESHLSVWLILIIFLIKSLLMIFMLIIIFEYGYTQFISNMRKYRQYFSSDISDSVSQQSLNDNQYKWYNVSPRIFGILILVIVKILKIPILYVQISYLKINGKISIHPLILISICISLFYFLSCLFLWFGLCVKNKWQFHINI----QTNPLPQIGVGKLKSSPEPIYGCFYN 435

[Close]

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Match: SMESG000008310.1 (SMESG000008310.1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.012e-18
Identity = 141/611 (23.08%), Postives = 250/611 (40.92%), Query Frame = 2

Query:  104 NCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIR-----SNFK--------FEKDGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIKISGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLT------MILGFLTIFDYGQTQ-------------------------SAISCLKGLKTFSKSYWLCLVIVVGFGLVFSTLIIELLMFIVFFQCFQMFHXXXXXXXXXXXXXXXXYQI----LAWFTFSIKNRWVFQL-DLPELTQGSSCNTDGNNKYTG----------------SPDTIYGCFHNNITLXXXXXXXXERGM-GRIIETPSAEN-GCYGTN--------YQNYVYLQNPTALIPTISEDYDENXXXXXXXXXINRKETRNNSLTDQDLIYRTASSISDIGLIKMKENLNSDSYIS-LQMNNSRKAGKLVTFHE 1708
            NC  LW+IW    + V  +Y+ Y  I RYI FK    +  +G+ +    +++Y+ CII+ ++ F F +Y  +    +  + NI+      NFK        ++ D   N     + L   L R  LPY+ I+H + A C L+PIP +E  QI  + L  + IW  + DF  D   W    S  D N      ++ K      ++ N I   + LV+++P+  W  +K FS  FS ++   SL  +I Y T  V I+++ N      P+ + ++++  T  S +++  L+      ++L F  +F++G  Q                         S I+C K    + + Y      +  FG +F   I  ++   +F+    + +       ++L ++  +  I    +AWF F++K  W F      +     S  T  N+   G                 P+ +Y C+H               GM  R++      N  CY             NYVYLQ  + L PTI+     N     +        + ++++  ++++  + SS      I+ +  ++ D Y    Q  NSR AG ++ + +
Sbjct:    6 NCLRLWRIWNFIFLVVLYIYLVYDCIFRYIKFKKYSLDLGFGSNYSAVHMNIYLICIISGLTCFPFSLYCILFFNGNYADDNIKVGKDTDNFKIHQENDVTWQPDELTNLEKSKNILWKRLKRHFLPYSFIMHFVSAICFLVPIPFLEAHQIHQKVLKPNEIWHSDLDFLWDIDSW---HSDFDNNGRWRTTWLMKP-----QYFNLIFCLLCLVLKYPNVFWACHKGFSFTFSLLLTLISLHVIIDYSTTCVLIRVASN------PEFVAEKLRI-TFLSPISLMLLSILGSSVLLLTFWCVFEFGFHQFLDNLSRYRHLMLIQCDGSHDGSNMSDITCCKRKDLYLRGY------LPHFGAIFGCAIGFIMKTPLFYASISVIYHHTSDPLMLLNLLLAVLFICAWFIAWFGFTLKPNWRFIFPSCSKAISDVSSTTIANSMTAGVPQPSLHSRELGGRADQPNQVYSCYH---------------GMKSRLVMDERLRNPDCYSEENDLSNNAANNNYVYLQ--STLNPTINHILMSNNMPQLQPGESPLMVSFHDNIDSRNMVSESESSYDSASNIRNQVLISVDPYHGRFQSLNSRTAGGVLKYSD 578

[Close]

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Match: SMESG000005995.1 (SMESG000005995.1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.142e-13
Identity = 71/262 (27.10%), Postives = 116/262 (44.27%), Query Frame = 2

Query:  104 NCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIR------------SNFKFEK-------DGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIK 832
            NC +L KIWYGF I  FQ+YV Y  I  Y+ FK Q  +PRYG+ W+  PL   +   + S +L   F+Y C+I   +  N N+              +  FE+         +  C+      V        P   +LH I A  LL+P   +  E+ +N AL+ +++W+ +            + ++ D      +   ++S +  I  INF +A++ L +   S  W + K FSI  +      S  +LI+    S+ I+
Sbjct:   11 NCRSLGKIWYGFTILAFQIYVHYDEINNYLDFKKQSNDPRYGSEWNENPLTFCLMMTMFSFALLPIFLYTCLIKTGNLINDNVDLGETNTLQILQPDSLIFERIQNCHDDSAKPKCWNIFKYYV-------FPSCQLLHYISAFLLLVPHLYLNAEKFKNSALSYENLWISDLHL---------IFNVRDPGTLTIVKQESESLSFPISTINFFIASMFLALFTSSVFWKTCKSFSILIAIFDLVLSFGYLILNSVLSIVIR 256

[Close]

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Match: SMESG000005995.1 (SMESG000005995.1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.362e-13
Identity = 71/262 (27.10%), Postives = 116/262 (44.27%), Query Frame = 2

Query:  104 NCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNLWHMAPLHLYIGCXXXXXXXXXXXXXXCVISVRSSPNGNIR------------SNFKFEK-------DGQKNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEALTSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINFIVAAVILVIQFPSGLWYSNKCXXXXXXXXXXXNSLTWLIIYETGSVFIK 832
            NC +L KIWYGF I  FQ+YV Y  I  Y+ FK Q  +PRYG+ W+  PL   +   + S +L   F+Y C+I   +  N N+              +  FE+         +  C+      V        P   +LH I A  LL+P   +  E+ +N AL+ +++W+ +            + ++ D      +   ++S +  I  INF +A++ L +   S  W + K FSI  +      S  +LI+    S+ I+
Sbjct:   11 NCRSLGKIWYGFTILAFQIYVHYDEINNYLDFKKQSNDPRYGSEWNENPLTFCLMMTMFSFALLPIFLYTCLIKTGNLINDNVDLGETNTLQILQPDSLIFERIQNCHDDSAKPKCWNIFKYYV-------FPSCQLLHYISAFLLLVPHLYLNAEKFKNSALSYENLWISDLHL---------IFNVRDPGTLTIVKQESESLSFPISTINFFIASMFLALFTSSVFWKTCKSFSILIAIFDLVLSFGYLILNSVLSIVIR 256

The following BLAST results are available for this feature:

BLAST of Lysine-specific demethylase 6A vs. Ensembl Human
BLAST of Lysine-specific demethylase 6A vs. Ensembl Celegans
BLAST of Lysine-specific demethylase 6A vs. Ensembl Fly
BLAST of Lysine-specific demethylase 6A vs. Ensembl Zebrafish
BLAST of Lysine-specific demethylase 6A vs. Ensembl Xenopus
BLAST of Lysine-specific demethylase 6A vs. Ensembl Mouse
BLAST of Lysine-specific demethylase 6A vs. UniProt/SwissProt
BLAST of Lysine-specific demethylase 6A vs. TrEMBL
BLAST of Lysine-specific demethylase 6A vs. Ensembl Cavefish
BLAST of Lysine-specific demethylase 6A vs. Ensembl Sea Lamprey
BLAST of Lysine-specific demethylase 6A vs. Ensembl Yeast
BLAST of Lysine-specific demethylase 6A vs. Ensembl Nematostella
BLAST of Lysine-specific demethylase 6A vs. Ensembl Medaka
BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST

BLAST of Lysine-specific demethylase 6A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1I8HYR5	1.258e-17	25.25	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A267H7U1	1.288e-15	25.75	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A267E669	1.422e-15	25.75	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A267GLJ3	1.560e-15	26.97	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1I8IPE7	2.529e-14	26.58	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]

BLAST of Lysine-specific demethylase 6A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Lysine-specific demethylase 6A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000030162.1	0.000e+0	99.35	SMESG000030162.1	[more]
SMESG000077784.1	5.591e-30	30.30	SMESG000077784.1	[more]
SMESG000008310.1	1.012e-18	23.08	SMESG000008310.1	[more]
SMESG000005995.1	2.142e-13	27.10	SMESG000005995.1	[more]
SMESG000005995.1	2.362e-13	27.10	SMESG000005995.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30005027 ID=SMED30005027|Name=Lysine-specific demethylase 6A|organism=Schmidtea mediterranea sexual|type=transcript|length=2110bp
CGATTTATCAACACACGGACATCACTGCGTTACATAATTCGATAATCATT
TTTATCATACTTTTTTATCCCATGTATTCATAAAAATGTATTCGAAACTC
TGGAATTGTAACAATTTGTGGAAAATCTGGTATGGATTTCTTATCAGTGT
ATTCCAACTCTATGTGACATACAGTTCAATTCGGCGATATATCATTTTCA
AAAGCCAATATTACAATCCTCGATACGGCAACTTGTGGCACATGGCACCG
CTTCACCTCTATATCGGATGTATAATTGCATCTATTTCTCTATTTCTGTT
TTTCATATATTTCTGCGTCATCAGCGTTCGAAGTTCGCCTAATGGAAATA
TCAGATCGAATTTCAAATTTGAAAAAGATGGACAAAAAAATTGTTTTGGA
CCAATTTCGAGATTGGTATCGAGTTTAATGCGTGACAATCTTCCCTATGC
ACCGATTTTACATTTAATCATGGCGGCTTGTTTGCTTTTGCCAATTCCAG
TAGTTGAAATGGAACAAATACGAAACGAAGCTCTTACTTCAGATTCTATT
TGGATGGTGAACTTCGACTTTTTTTCTGATCAATTTCGATGGTTGACGAT
GGAGTCTTTGGGGGATGGGAATCCTTATAAGAATATCCACTACATCAACA
AAAGTAGTACCTTGAGTATTGAATTCATTAACTTTATCGTTGCCGCCGTT
ATACTTGTGATCCAATTTCCTAGCGGTTTATGGTATTCCAACAAGTGCTT
TTCTATTTTCTTTTCGAGCATTATATTTTTCAATTCTTTAACTTGGCTCA
TTATCTATGAAACTGGCTCAGTCTTCATAAAAATTTCCGGCAATATTGGC
TCATTCAATATTCCTCAAAAATTGGGAGATCAAATCCAATATGGAACTAT
GTTTTCATTTGTTGCGGTCAATTTCCTGACAATGATCCTGGGATTTCTGA
CGATTTTCGATTATGGACAAACTCAGTCAGCGATCAGTTGTTTGAAAGGT
CTGAAAACCTTCAGCAAGAGTTATTGGCTCTGTTTAGTAATAGTCGTGGG
GTTTGGTTTGGTTTTCTCCACTTTAATAATTGAGTTGTTGATGTTCATTG
TATTTTTTCAATGTTTCCAAATGTTCCATATCCCAATATTTCTGATATCA
ATCGTATTAGGAATAATCAACATAATTTATCAAATTCTCGCCTGGTTTAC
TTTTTCAATTAAAAATCGGTGGGTTTTCCAGCTTGATCTACCAGAACTCA
CTCAAGGTTCCAGTTGCAATACTGATGGAAATAACAAATATACTGGATCA
CCGGATACGATTTACGGTTGCTTTCACAACAACATAACTCTGAGCACTTC
TTCTTCTCATTCATCGGAACGGGGAATGGGGAGAATAATTGAGACCCCGA
GTGCTGAAAACGGTTGTTATGGAACGAATTATCAAAACTATGTCTACTTA
CAAAACCCCACAGCTCTTATACCGACGATTTCAGAAGATTACGATGAGAA
CACAACAAGCACTAGTAAATCTTCATCTATAAATAGGAAAGAAACGAGAA
ACAATTCTCTGACTGACCAAGACCTAATCTATCGTACTGCTTCGTCAATA
TCCGACATCGGTTTGATAAAAATGAAAGAAAATTTAAACTCAGATTCCTA
TATAAGTTTACAAATGAATAACTCTCGAAAAGCTGGAAAGTTGGTGACAT
TCCACGAGATTGGTGAGGAGAAAATCCCATTTTTCGGGAGTTACAATGAT
CAGTATTCGAGTAACAACATGGAACAATTAGATTTTCTCAAAGTTCCTGA
TTTAAACAACCTAAACCGGTCGACATTGTCTGGATCACAATCAGAGTCCA
GCTTGGACCAAATCAGCAGCTCCTTCTCATCGAAACAGGCCATAGTGTCC
AACGAGAACGGCCTGTGTAGTGAAGTGTAAATCCTATTATTTTCTCTGTA
TTATTTCATTCGTTTTTGTAAATTTGCCTATCATGTTTGTGTTATTGTTT
TTTATAATATTTTTCGTATCATTTGTTATTAGTTTCCCTCAGGGTTCGGG
ATAAATTGTGACCAAATATAAAAATCGGCCAATCTAAGTGAGGAAGAAAG
AAAGATGGAG

protein sequence of SMED30005027-orf-1

>SMED30005027-orf-1 ID=SMED30005027-orf-1|Name=SMED30005027-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=615bp
MYSKLWNCNNLWKIWYGFLISVFQLYVTYSSIRRYIIFKSQYYNPRYGNL
WHMAPLHLYIGCIIASISLFLFFIYFCVISVRSSPNGNIRSNFKFEKDGQ
KNCFGPISRLVSSLMRDNLPYAPILHLIMAACLLLPIPVVEMEQIRNEAL
TSDSIWMVNFDFFSDQFRWLTMESLGDGNPYKNIHYINKSSTLSIEFINF
IVAAVILVIQFPSGLWYSNKCFSIFFSSIIFFNSLTWLIIYETGSVFIKI
SGNIGSFNIPQKLGDQIQYGTMFSFVAVNFLTMILGFLTIFDYGQTQSAI
SCLKGLKTFSKSYWLCLVIVVGFGLVFSTLIIELLMFIVFFQCFQMFHIP
IFLISIVLGIINIIYQILAWFTFSIKNRWVFQLDLPELTQGSSCNTDGNN
KYTGSPDTIYGCFHNNITLSTSSSHSSERGMGRIIETPSAENGCYGTNYQ
NYVYLQNPTALIPTISEDYDENTTSTSKSSSINRKETRNNSLTDQDLIYR
TASSISDIGLIKMKENLNSDSYISLQMNNSRKAGKLVTFHEIGEEKIPFF
GSYNDQYSSNNMEQLDFLKVPDLNNLNRSTLSGSQSESSLDQISSSFSSK
QAIVSNENGLCSEV*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000020	protonephridia
PLANA:0000418	head
PLANA:0002111	X2 cell

Vocabulary: **molecular function**
Term	Definition
GO:0008168	methyltransferase activity
GO:0046872	metal ion binding
GO:0071558	histone demethylase activity (H3-K27 specific)

Vocabulary: **biological process**
Term	Definition
GO:0010468	regulation of gene expression
GO:0032259	methylation
GO:0071557	histone H3-K27 demethylation

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane
GO:0016021	integral component of membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 468..488
None	No IPR available	PANTHER	PTHR21579:SF13	PROTEIN TINCAR	coord: 8..397
None	No IPR available	PANTHER	PTHR21579	FAMILY NOT NAMED	coord: 8..397
None	No IPR available	TMHMM	TMhelix		coord: 57..79
None	No IPR available	TMHMM	TMhelix		coord: 226..248
None	No IPR available	TMHMM	TMhelix		coord: 15..37
None	No IPR available	TMHMM	TMhelix		coord: 351..373
None	No IPR available	TMHMM	TMhelix		coord: 317..339
None	No IPR available	TMHMM	TMhelix		coord: 269..291
None	No IPR available	TMHMM	TMhelix		coord: 192..211
None	No IPR available	TMHMM	TMhelix		coord: 119..141

Main menu

Lysine-specific demethylase 6A

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

blazegraph-runner