SMED30004994
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004994 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30004994 aligns in the following genomic locations:
Homology
BLAST of SMED30004994 vs. Planmine SMEST
Match: SMESG000050292.1 (SMESG000050292.1) HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0 Identity = 409/442 (92.53%), Postives = 414/442 (93.67%), Query Frame = 2 Query: 53 MTFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDKFTINGKGFHLKKRSGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKYKNEMIILLFLDQDGSVSVESKNSNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVFDGQQIQTHIYHINSLLSLINVGFCHVNYLRNFAGKMRFRYNSLDWRPLINENGKSTPNPISKGTTSLNYIYSVL---ASFIIFLAFIVYIRKRHN 1369 MTFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDK SGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKY NEMIILLFLDQDG VSVESKNSNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKV+DGQQIQTHIYHINSLLSLINVGFCHVNYLRNFAGKMRFRYNSLDWRPLINENGKSTPNPISKGTTSLNYIYS+L FI+ L Y ++ N Sbjct: 1 MTFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDK-------------SGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKYNNEMIILLFLDQDGYVSVESKNSNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVYDGQQIQTHIYHINSLLSLINVGFCHVNYLRNFAGKMRFRYNSLDWRPLINENGKSTPNPISKGTTSLNYIYSILFGIGVFILLLCLSNYYCRKEN 429
BLAST of SMED30004994 vs. Planmine SMEST
Match: SMESG000050295.1 (SMESG000050295.1) HSP 1 Score: 323.553 bits (828), Expect = 2.448e-104 Identity = 207/517 (40.04%), Postives = 283/517 (54.74%), Query Frame = 2 Query: 56 TFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDKFTINGKGFHLKKRSGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKYKNEMIILLFLDQDGSVSVESKNSNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVFDGQQIQTHIYHINSLLSLINVG-FCHVNYLRNFAGKMRFRYNSLDWRPLINENGKSTPNPISKGTTSLN---YIYSVLASFIIFLAFIVYIRKRHNGKEANEIINNQNREDISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVITESETNG 1594 + ++ L ++ N+F + A+YIY+E L F +Y+ G++ Y D EN + +K++ + + + +N + T + CS ++++ F L S + I+C N + L+ T KD F +P+P +K LFIWMGNIG YIG Y PL+ Q++GC D ++ K L+++ G F+ CDI Y E N + ++ KMY+C+ S+N YC EK K S I RI+W NYCLLE+ KN I++L +D+ + + K+S + IN + N N LL+ + +KV G+ + + S +I VG + H N F G + R + + S+ NP L I+ VLASF+I LAFIVYIRKRHNGKEANEIINNQNRE+ISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVIT+SETNG Sbjct: 3 SVKLILLFITYLNIFLWLNASYIYKELAGTIADHLGLEFRIYILNGSGIIFYSFDVENDIFVKIDVDRPKPELIVAYNQKIPSKISTMNEICGCSACFNIFMTFKFHLDLSLIDIECANKSNIILLKNTTDKDSFKFFPRPKSYKSSEKILFIWMGNIGKYIGYSGMKYLPLKAQIFGCLDYIKVDKKYVKLERQEGVSFLNSCDILYNEKVCYKSNCWQNKNSYKMYDCSVASLNTKYCNITFSRHYEKEFDSRKNS--ERIKLMRIDWIRDQHIMK-NYCLLEIILKNGTILMLHIDRHNHLRMNIKHSK-TKLLINTNL---NKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISIRLEMILF---------SSQNPSRYSILDLKEIFIIFLVLASFLILLAFIVYIRKRHNGKEANEIINNQNREEISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVITDSETNG 503
BLAST of SMED30004994 vs. Planmine SMEST
Match: SMESG000050295.1 (SMESG000050295.1) HSP 1 Score: 203.756 bits (517), Expect = 8.969e-60 Identity = 132/258 (51.16%), Postives = 159/258 (61.63%), Query Frame = 2 Query: 833 SSEIDYFRIEWKNSASKNSINYCLLEMKYKNEMIILLFLDQDGSVSVESKNSNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVFDGQQIQTHIYHINSLLSLINVG-FCHVNYLRNFAGKMRFRYNSLDWRPLINENGKSTPNPISKGTTSLN---YIYSVLASFIIFLAFIVYIRKRHNGKEANEIINNQNREDISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVITESETNG 1594 S I RI+W NYCLLE+ KN I++L +D+ + + K+S + IN + N N LL+ + +KV G+ + + S +I VG + H N F G + R + + S+ NP L I+ VLASF+I LAFIVYIRKRHNGKEANEIINNQNRE+ISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVIT+SETNG Sbjct: 150 SERIKLMRIDWIRDQHIMK-NYCLLEIILKNGTILMLHIDRHNHLRMNIKHSK-TKLLINTNL---NKISNSLLVNLDPSNFSVKVSFGEGRVEYRDEMTSEYVIITVGKWQHSNIYPEFLGNISIRLEMILF---------SSQNPSRYSILDLKEIFIIFLVLASFLILLAFIVYIRKRHNGKEANEIINNQNREEISEDENNVYGIYSHNQNPTEEDRQIVIGNVASSSLGVYDIYIPQIISNEYNVITDSETNG 393
BLAST of SMED30004994 vs. Planmine SMEST
Match: SMESG000050289.1 (SMESG000050289.1) HSP 1 Score: 183.726 bits (465), Expect = 3.148e-51 Identity = 150/514 (29.18%), Postives = 245/514 (47.67%), Query Frame = 2 Query: 14 IKYYLFKIIMK*IMTFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDKFTINGKGFHLKKRSGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKYKNEMIILLFLDQDGSVSVESKN--SNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVFDGQQIQTHIYHINSLLSLINVGFCHVN-YLRNFAGKMRFRYNSLDWRPLINENGKS--------TPNPISKGTTSLNYIYSVLASFIIFLAFIVYIR-------KRHNGKEANEIINNQN--REDISEDENNVYGIYSHNQNPT-EEDRQIV 1492 +KY+LF + LL+ FN +F + YIY++FD + + F+V+++ FKGVL Y +DS+N+LLI++N++ I FNS +T L CS +VYL ++ L + E+ I C K + T DEF YP P + K + WMGNIGP+ G + Y L +QL GC DKF NGK KK SG F C++ Y+E + + KMY+C+ SMN +C+T D++ SY ++ E+ YFR++W+ + + L+E+K K+E ++L ++D ++ + + N +N + ++K + + R LLI + IK+ D I T+I LIN+G + F GK+ F++N W+ NG S PN + S Y+ ++ II + I+ + KR + K+ + I N + E +S + Q+PT + D +I+ Sbjct: 5 VKYFLF-----------LSVLLSKFNGMF----SEYIYEKFDGNMVSDKGMNFSVFIKNFKGVLFYCVDSKNRLLIQINKKSTETIITFKFNSYVTSEDMTSKRLCKCSNCSNVYLQLSILLQQLEILINCNKQANVVKFNKETDSDEFSEYPTPPDYNNNQTKTYFWMGNIGPFTGSKNKIYLALTSQLSGCLDKFYFNGKNIKFKK-SGKIFQSFCEVLYKEKVCNESRCSNNETHYKMYDCSPLSMNGKHCETILSDYAIVQKSYIRKQRWKEVIYFRLDWQMEVNSKIKRFSLMEIKLKDETLLLFYIDSTRNIVMSTLNISANTDIVDFSMKSDLNH--RFTLLIILGESETTIKLNDCSNILTYIKKNIFEFVLINIGTIQSDPSFSLFEGKINFQFNPKYWKI---SNGNSDFFLTSTIIPNRVQPSNLSKYYLCVIVFGGIIIMLVIILRKCKFRQKMKRQSQKDKWKDIQNNSYESESVSTPSAKNLVLEPFTQSPTLDSDYEII 497
BLAST of SMED30004994 vs. Planmine SMEST
Match: SMESG000050289.1 (SMESG000050289.1) HSP 1 Score: 168.703 bits (426), Expect = 5.520e-46 Identity = 136/462 (29.44%), Postives = 221/462 (47.84%), Query Frame = 2 Query: 170 FNVYLQTFKGVLLYGLDSENKLLIKVNREMEFRTIHIIFNSEEIGIKLTKTTLKFCSLNEDVYLDFTLRLSRSELTIKCRNMTWSYKLRQNTFKDEFDLYPQPNHDFKKKKKLFIWMGNIGPYIGRDSAHYKPLQNQLYGCTDKFTINGKGFHLKKRSGNQFIFPCDINYQETEYKGKNVTKRLSTIKMYNCTHYSMNPLYCQTNPLDFSEKTHSYHKQSMSSEIDYFRIEWKNSASKNSINYCLLEMKYKNEMIILLFLDQDGSVSVESKN--SNDPNIQINLKIFMPNFKRNVLLIFTTNIDLIIKVFDGQQIQTHIYHINSLLSLINVGFCHVN-YLRNFAGKMRFRYNSLDWRPLINENGKS--------TPNPISKGTTSLNYIYSVLASFIIFLAFIVYIR-------KRHNGKEANEIINNQN--REDISEDENNVYGIYSHNQNPT-EEDRQIV 1492 F+V+++ FKGVL Y +DS+N+LLI++N++ I FNS +T L CS +VYL ++ L + E+ I C K + T DEF YP P + K + WMGNIGP+ G + Y L +QL GC DKF NGK KK SG F C++ Y+E + + KMY+C+ SMN +C+T D++ SY ++ E+ YFR++W+ + + L+E+K K+E ++L ++D ++ + + N +N + ++K + + R LLI + IK+ D I T+I LIN+G + F GK+ F++N W+ NG S PN + S Y+ ++ II + I+ + KR + K+ + I N + E +S + Q+PT + D +I+ Sbjct: 9 FSVFIKNFKGVLFYCVDSKNRLLIQINKKSTETIITFKFNSYVTSEDMTSKRLCKCSNCSNVYLQLSILLQQLEILINCNKQANVVKFNKETDSDEFSEYPTPPDYNNNQTKTYFWMGNIGPFTGSKNKIYLALTSQLSGCLDKFYFNGKNIKFKK-SGKIFQSFCEVLYKEKVCNESRCSNNETHYKMYDCSPLSMNGKHCETILSDYAIVQKSYIRKQRWKEVIYFRLDWQMEVNSKIKRFSLMEIKLKDETLLLFYIDSTRNIVMSTLNISANTDIVDFSMKSDLNH--RFTLLIILGESETTIKLNDCSNILTYIKKNIFEFVLINIGTIQSDPSFSLFEGKINFQFNPKYWKI---SNGNSDFFLTSTIIPNRVQPSNLSKYYLCVIVFGGIIIMLVIILRKCKFRQKMKRQSQKDKWKDIQNNSYESESVSTPSAKNLVLEPFTQSPTLDSDYEII 464 The following BLAST results are available for this feature:
BLAST of SMED30004994 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004994 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004994 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004994 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004994 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004994 ID=SMED30004994|Name=SMED30004994|organism=Schmidtea mediterranea sexual|type=transcript|length=1685bpback to top protein sequence of SMED30004994-orf-1 >SMED30004994-orf-1 ID=SMED30004994-orf-1|Name=SMED30004994-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=515bp MTFQIYFLLTSFNNLFNIILATYIYQEFDNVSLKRDNLIFNVYLQTFKGVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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