Anosmin-1

Overview
NameAnosmin-1
Smed IDSMED30004938
Length (bp)3264
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Anosmin-1 (SMED30004938) t-SNE clustered cells

Violin plots show distribution of expression levels for Anosmin-1 (SMED30004938) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Anosmin-1 (SMED30004938) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Anosmin-1 (SMED30004938) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004938

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004938h1SMcG0020575 SmedASXL_013895SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30004938h1SMcG0020575 SmedASXL_013896SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30004938h1SMcG0020575 Contig35362uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004938h1SMcG0020575 Contig35362newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30004938h1SMcG0020575 dd_Smed_v6_10075_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004938h1SMcG0020575 dd_Smed_v4_10075_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Anosmin-1 vs. Ensembl Human
Match: ANOS1 (anosmin 1 [Source:HGNC Symbol;Acc:HGNC:6211])

HSP 1 Score: 151.369 bits (381), Expect = 4.833e-37
Identity = 166/675 (24.59%), Postives = 278/675 (41.19%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKI--SWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV--YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLV-------FWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            D  + + +V +A C  +CL +Q        Q  Q++       S++  Q    N +  + C   C+ SG   L    C + C+P     S  CL  C+FL   + +  G+CP   +           C +D +C     V+KCC+      C VP+   K G  L P   +   +  S ++  K+  + F+  + P++YV+Q R  +        A++W+T+  T  E V L D++P  WYQFRVA+V  +GT GF + S   + +K P++P  P NL      + ++G + + + W  P   DIPV  YK+  SW+       P  +     TD      G Q S  +E L  D  Y V+++AI  WG   L S   ++  T+        E+LV      +          P    +++  P+Y    L++ ++   ++   V  Y + + P  C    ++       + S    +E Y             L  L FSC+Y + VQP  IRP      + V            F TP C  L  +       + CL  A  +L   +K+L +  + +  SF+V        + +      L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +T Y ++VQ +
Sbjct:   36 DESLSAGSVQRARCASRCLSLQITRISAFFQHFQNN------GSLVWCQ----NHKQCSKCLEPCKESGD--LRKHQCQSFCEPLFPKKSYECLTSCEFLKYILLVKQGDCPAPEKASGFAAACVESCEVDNEC---SGVKKCCSNGCGHTCQVPKTLYK-GVPLKPRKELRFTELQSGQLEVKWS-SKFNISIEPVIYVVQRRWNYGIHPSEDDATHWQTVAQTTDERVQLTDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSAPPAPANLRLANSTVNSDGSVTVTIVWDLPEEPDIPVHHYKVFWSWMVSSKSLVPTKKKRRKTTD------GFQNSVILEKLQPDCDYVVELQAITYWGQTRLKSAKVSLHFTSTHA-TNNKEQLVKTRKGGIQTQLPFQRRRPTRP-LEVGAPFYQDGQLQVKVYWKKTEDPTVNRYHVRWFPEACAHNRTTG-----SEASSGMTHENY-----------IILQDLSFSCKYKVTVQP--IRPKSHSKAEAV-----------FFTTPPCSALKGKSH---KPVGCLGEAGHVL---SKVLAKPENLS-ASFIVQDVNITGHFSWKMAKANLYQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPSDHYVLTVPNLRPSTLYRLEVQVL 637          
BLAST of Anosmin-1 vs. Ensembl Celegans
Match: kal-1 (KALlmann syndrome homolog [Source:UniProtKB/TrEMBL;Acc:G5EDX1])

HSP 1 Score: 98.5969 bits (244), Expect = 1.047e-20
Identity = 109/452 (24.12%), Postives = 189/452 (41.81%), Query Frame = 2
Query:  299 INPDHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQC--QPNSSGCLEGCQFLNTAIKMSLGNCPE-----KFEIDKL--LDRECHLDQQCMIEGDVRKCCNLRCVVPQFHP-KIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPI-LYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDL-QPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHG-----------------FTQPDGQSLEALTD--FVQ---------TVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKIS 1534
            ++PD ++  +  C+A C H   Q   N+      M+   T+ H     I Q CK+      ++C+  C          K C   C    +   C + C +L    +   G CP       +E   L  +D EC   Q+C   G     C+ +C+ P+    K+ P     ++ E K   S  + W     S ++      L+++Q R   + D   +  + W+T+ +  +    L+ L  PG +Y+FR A+V++ G+ GF S S P K++K   +P  P++L+ GA ++ A+G  N  VHW  P + D+P+  Y+ISW                      F   + +SL++  D  F+           VP +  S +I GL  +  Y V++ A        L      +F+ T      +S   V +D    +G +     G ++ +I+ S
Sbjct:   13 VSPDELI--STRCQAQCLHLMDQRLQNN------MELRKTLKHH----IIQLCKE--DATCSACSAPCREQFDDI---KACKKSCVASDDRETCEDSCHYLQRIYQEKPGACPSVSNQSNYECSALCQMDGECPETQKCCSSG-----CSRQCLKPRSSDIKLLPIPRNISVQERKRKRSIIIRWATGRLSKAQQNENANLFLVQWRWGLHAD--ESAMTEWQTVTVRNKPYAILKHLLSPGRFYEFRTAAVSQEGSLGFSSPSKPFKISKEAKAPPPPKDLSLGASKVVASGLWNQMVHWNPPPS-DLPIKNYQISWASSTKSEADAFEEQMRKKSTLEFAAHEKRSLQSDDDEEFIGIQERDRQSVVVPSHATSSEITGLFPNSVYLVEIHASVDSSDGELHGEKGVVFIRTA-----DSLSAVPEDREPYTGPKARIAHGSEELKIEAS 434          
BLAST of Anosmin-1 vs. Ensembl Fly
Match: Kal1 (gene:FBgn0039155 transcript:FBtr0084549)

HSP 1 Score: 69.707 bits (169), Expect = 8.910e-12
Identity = 75/339 (22.12%), Postives = 135/339 (39.82%), Query Frame = 2
Query:  488 CTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKL----LDRECHLDQQCMIEGDVRKCCNLRC---VVPQFHPKIGPSLWP-PTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKAS--NWKTLLITFREGVN-------LQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLT------DGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPV 1435
            C   C  + ++  G + C N+C                 +    G+CP+     +     LD  C  D +C    +V+KCC   C    V     +    L P P I+  +      +  K + +     +L   + ++VR++  R F   K     W+ +  T  E +           ++PG WY+ RVA+V  YG  G+   S P      P  P  P +        DG +       + +++ W  P+  ++PV +YKI+W     +      S+   + +V+    +   ++I+ L+ + +Y +QV+AI   GS  L S   ++   T   P+
Sbjct:   64 CLAKCGSNPTTKAGREQCLNKC------------IQELLLGPRAGSCPKIGRQSRARLSCLD-NCQYDHEC---PEVQKCCPSSCGPMCVEPLGVRNNTQLPPIPKILYFRRSRGHAVDLKIESS-----LLVYYFHVEVRSHIGRHFAARKLGPWQWQKVEKTMEENIGHSKHTYIFFHMRPGRWYEVRVAAVNAYGFRGYSEPSDPFPSTGNPKPPKSPNDSKIIGKQFDGRY-------MTLKLVWC-PSKSNLPVEKYKITWSL---YVNSAKASMITNSSYVK----DTHQFEIKELLPNSSYYIQVQAISYLGSRRLKSEQWSMLFNTTLQPL 366          
BLAST of Anosmin-1 vs. Ensembl Zebrafish
Match: anos1a (anosmin 1a [Source:ZFIN;Acc:ZDB-GENE-000201-9])

HSP 1 Score: 126.716 bits (317), Expect = 2.060e-29
Identity = 121/469 (25.80%), Postives = 191/469 (40.72%), Query Frame = 2
Query:  539 CSNQCQ---PNSSG-CLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPK---IGPSLWP--PTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNL-VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQ 1906
            C + C+   P   G C+  C+FL + + +  G+CP               C  D +C  +   +KCC   C      PK    G  L P    + E  +    E+ W    + F+    P+L VLQ R  +        A+ W+ +  T  E V L D++PG WYQFRVA+V  +GT G+ + S   + +K P  P  P +L        A+   ++R+ W   A  DIPV  YK+SW        P  +  E       TV G+    +++ L  + +Y V+++A+  WG   L S+   +  TT            S+  S L          PQ   + +  P+Y    L++ ++       +  Y++ +AP  C    S +                 +E+ IT     A+L  LQFSC+Y + +QP             V L     V +  F TPSC  + S+
Sbjct:   92 CRDLCEHAFPKRHGECVTSCEFLRSVMVVKQGDCPPPERASGFAAACVEGCEEDGECSGQ---KKCCPNGCGHTCQSPKNLYRGAPLKPRKELVFEELESGVLEVRW---SSKFNVSAEPVLNVLQRRWNYGIHPSEDGATEWQVVARTSEERVWLTDIRPGRWYQFRVAAVNVHGTRGYTTPSRHFRSSKDPPPPPAPSDLHVSDMTFGADHSASVRLSWSISADLDIPVNHYKLSWSSSDHTLPPKLKKRE-------TVNGDSTYAELDDLRENGSYTVELQAVSYWGQVPLKSSKAILQFTT------------SQTQSDLEPTVSPVFIKPQSDLLDVGTPFYQDGQLQVRVYWKKKDPTVNRYRVQWAPEFCSHNRSRT-----------------QEKLITQE-NFASLSGLQFSCKYKVIIQP-------------VGLKGRAQVESTTFYTPSCATIQSK 504          
BLAST of Anosmin-1 vs. Ensembl Zebrafish
Match: anos1b (anosmin 1b [Source:ZFIN;Acc:ZDB-GENE-000201-13])

HSP 1 Score: 125.946 bits (315), Expect = 2.962e-29
Identity = 137/600 (22.83%), Postives = 246/600 (41.00%), Query Frame = 2
Query:  572 CLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPS-LWPPTIMEGKDGSSF--------EMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLF----ITNSKKN-LVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANP----ILEPPNK--ILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKT 2302
            C+   +FL +      G+CP               C  D++C     V+KCC+  C     H    P+ L+    ++ +   SF        ++TW    + F+  + P+LYVLQ R          +AS W+T+L+T  +   L+D++P  WYQFRV++V   GT GF + S      + P  P  P+N       +  +G +++ V W  P   D+ V  +K++W        P G    + +   +   G+    +++GL  +  Y  Q++AI  WG   L S+   I  TT       S+   +  +   S +       P   R++ + P+YH N L++ +F    +  S+K+   Y + + P VC                    N    E+  T    +  +  L F+C+Y + V+P               + +++  +T    TP CL L+ + +  +   R     +P     L  P K  +LFQ  + + ++ L  W+ + +S     P+  F+  W          K      N  L+       ++T +L  +  S+ ++ L+ ++ Y VQVQ +        ++KT
Sbjct:   99 CVTSAEFLRSLRVQKQGDCPPAQRASGFAAACVESCARDREC---SGVKKCCSNGC----GHTCQSPANLFKGVPLKPRRDISFSEDHQGHVKVTWM---SKFNVSVEPVLYVLQRRWNHGIHPSEDEASAWQTVLMTMEDRSVLKDIRPHRWYQFRVSAVNSQGTRGFTTPSKHFFSVRDPLPPETPQNARTANQTLHPDGTVSVLVLWETPRESDLVVHHHKVTW-------SPRGTPSSSQSRAGRVTDGDVTQMELQGLQPNTPYTAQIQAISYWGQNRLKSSRAHISFTT------TSQNSATSSNGEFSNELPVSHSSPSVLRLEAAAPHYHDNQLQVKVFWKSRVQESQKDSSSYILRWYPEVCSH------------------NVTKDEKTATVQGTHYVITGLLFACKYRVAVKP---------------VAEQRSEVTTSVTTPLCLSLMGRRKKPAACAR--EERHPQLKKALRRPEKLAVLFQPVNGSLQA-LFSWQLSPESSG-QTPSSGFQFSWV---------KVSSGSSNGSLI-------SQTHILPPDQLSLTVDALQPSSVYRVQVQVLSEAGNGPSVAKT 622          
BLAST of Anosmin-1 vs. Ensembl Zebrafish
Match: anos1b (anosmin 1b [Source:ZFIN;Acc:ZDB-GENE-000201-13])

HSP 1 Score: 125.946 bits (315), Expect = 2.962e-29
Identity = 137/600 (22.83%), Postives = 246/600 (41.00%), Query Frame = 2
Query:  572 CLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPS-LWPPTIMEGKDGSSF--------EMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLF----ITNSKKN-LVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANP----ILEPPNK--ILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKT 2302
            C+   +FL +      G+CP               C  D++C     V+KCC+  C     H    P+ L+    ++ +   SF        ++TW    + F+  + P+LYVLQ R          +AS W+T+L+T  +   L+D++P  WYQFRV++V   GT GF + S      + P  P  P+N       +  +G +++ V W  P   D+ V  +K++W        P G    + +   +   G+    +++GL  +  Y  Q++AI  WG   L S+   I  TT       S+   +  +   S +       P   R++ + P+YH N L++ +F    +  S+K+   Y + + P VC                    N    E+  T    +  +  L F+C+Y + V+P               + +++  +T    TP CL L+ + +  +   R     +P     L  P K  +LFQ  + + ++ L  W+ + +S     P+  F+  W          K      N  L+       ++T +L  +  S+ ++ L+ ++ Y VQVQ +        ++KT
Sbjct:   99 CVTSAEFLRSLRVQKQGDCPPAQRASGFAAACVESCARDREC---SGVKKCCSNGC----GHTCQSPANLFKGVPLKPRRDISFSEDHQGHVKVTWM---SKFNVSVEPVLYVLQRRWNHGIHPSEDEASAWQTVLMTMEDRSVLKDIRPHRWYQFRVSAVNSQGTRGFTTPSKHFFSVRDPLPPETPQNARTANQTLHPDGTVSVLVLWETPRESDLVVHHHKVTW-------SPRGTPSSSQSRAGRVTDGDVTQMELQGLQPNTPYTAQIQAISYWGQNRLKSSRAHISFTT------TSQNSATSSNGEFSNELPVSHSSPSVLRLEAAAPHYHDNQLQVKVFWKSRVQESQKDSSSYILRWYPEVCSH------------------NVTKDEKTATVQGTHYVITGLLFACKYRVAVKP---------------VAEQRSEVTTSVTTPLCLSLMGRRKKPAACAR--EERHPQLKKALRRPEKLAVLFQPVNGSLQA-LFSWQLSPESSG-QTPSSGFQFSWV---------KVSSGSSNGSLI-------SQTHILPPDQLSLTVDALQPSSVYRVQVQVLSEAGNGPSVAKT 622          
BLAST of Anosmin-1 vs. Ensembl Zebrafish
Match: ANOS1 (anosmin-1-like [Source:NCBI gene;Acc:100535716])

HSP 1 Score: 111.694 bits (278), Expect = 7.617e-26
Identity = 91/320 (28.44%), Postives = 145/320 (45.31%), Query Frame = 2
Query:  320 LSAAVCKASCYHKCLQMQANS-SQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQ----PNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGD---VRKCCNLRCVVPQFHPK---IGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTK--ASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQ 1240
            LS +V +A C  +CL +     S     +Q + ++         +QC K        C   C    S  L    C + C+         CL  C+FL +   +  G+CP +   +K    E    Q C  + D   ++KCC+  C      PK    G  L P   ++  +  S ++  ++  + F+  + P+LYVLQ R  +N     T+  A++W+ +     E V L D++P  WYQFRVA+V  +GT GF + S   +  + P+ P  P  L      + A+G +++R+HW  PA  D+PV  YKI W   H  T+
Sbjct:   28 LSGSVQRARCASRCLSLHITRISAAFRHLQSNGSLVWCEN---HKQCSK--------CLEPCR--ASWDLKENQCQDLCETQFPKKHYECLTSCEFLRSVQTLKQGDCPAE---EKASGFEAACVQSCDSDSDCTSLKKCCSNGCGHTCQTPKNVYKGSPLKPRKDLQFVEHPSGQLELRWS-SRFNVSVEPVLYVLQRR--WNSGIHPTEDHATHWRDIAQVSEERVVLSDVRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSHRDPSPPPCPSALRVVNMSVCADGLLSLRIHWSLPAESDVPVHHYKIFWSSTHTPTK 328          
BLAST of Anosmin-1 vs. Ensembl Xenopus
Match: psmc4 (proteasome 26S subunit, ATPase 4 [Source:Xenbase;Acc:XB-GENE-5855370])

HSP 1 Score: 157.532 bits (397), Expect = 2.181e-39
Identity = 156/626 (24.92%), Postives = 262/626 (41.85%), Query Frame = 2
Query:  467 NQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR----IKISEPYYHKNSLKIDLFITNSKKNL--VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPIL-----EPPN-KILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            N +  + C   C+ +    L   +C N C+P        CL  C+FL + + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P    T  E + G   ++ W    + F+  + P++YV+Q R  +        ASNW+T+     E V L D++P  WYQFRVA+V  +GT GF + S   + +K P+ P  P NL          G +++R+ W  P   DIPV  YK+ W     +T      + A     +T  G+Q S  ++GL A+  Y V+++AI  WG   L S   +++ +T   P   S   +SK    +S  +   +  PQ  R    ++I  P+Y  N L++ ++   ++ ++  +Y + + P  C   E+                    E+ +T    +  L  L FSC+Y + V P R +             D     +  F TP C  L  +   Q   + C +   P++     +P N    F     N   +   W+ ++ +  L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   28 NHKQCSKCLEPCKETWD--LRKNNCHNLCEPLFPKKHYECLTSCEFLKSVLSVKQGDCPAPEKASGFAAACVESCDADNEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELTFDEPQPGH-LDIKW---SSKFNISVEPVIYVVQRRWNYGIHPSEDDASNWQTMAQITDERVQLSDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSPPPAPTNLRISNLTTNNEGTVSVRITWDLPEEPDIPVHHYKVFW----SWTANRKSVVPAKKKRRKTTEGSQNSVALDGLQANSNYMVELQAITYWGQVRLKSGKVSLYFSTS--PPSTSLDQISK----ISKMKTDILAPPQRKRTARMLEIGAPFYQDNQLQVKVYWKKTEDSMLGLYHVQWFPESCAHNETKG-----------------PEKAVTQDH-HIVLPALLFSCKYKVTVHPARPK-------------DHLKAESIFFTTPPCSTLKGKTHKQ---INCPSDTAPVVSKVLAKPENLSATFNVQEANITGYFS-WQLSKVN--LHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYMLTVHNLRPSMLYKLEVQVL 584          
BLAST of Anosmin-1 vs. Ensembl Xenopus
Match: psmc4 (proteasome 26S subunit, ATPase 4 [Source:Xenbase;Acc:XB-GENE-5855370])

HSP 1 Score: 157.918 bits (398), Expect = 2.333e-39
Identity = 165/679 (24.30%), Postives = 281/679 (41.38%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR----IKISEPYYHKNSLKIDLFITNSKKNL--VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPIL-----EPPN-KILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            D  + + ++ +A C  +CL +         +  Q++       S+I  Q    N +  + C   C+ +    L   +C N C+P        CL  C+FL + + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P    T  E + G   ++ W    + F+  + P++YV+Q R  +        ASNW+T+     E V L D++P  WYQFRVA+V  +GT GF + S   + +K P+ P  P NL          G +++R+ W  P   DIPV  YK+ W     +T      + A     +T  G+Q S  ++GL A+  Y V+++AI  WG   L S   +++ +T   P   S   +SK    +S  +   +  PQ  R    ++I  P+Y  N L++ ++   ++ ++  +Y + + P  C   E+                     +G   A  +  L  L FSC+Y + V P R +             D     +  F TP C  L  +   Q   + C +   P++     +P N    F     N   +   W+ ++ +  L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   41 DESLSAQSIYRARCTSRCLSLHITRISAYFKHFQNN------GSLIWCQ----NHKQCSKCLEPCKETWD--LRKNNCHNLCEPLFPKKHYECLTSCEFLKSVLSVKQGDCPAPEKASGFAAACVESCDADNEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELTFDEPQPGH-LDIKW---SSKFNISVEPVIYVVQRRWNYGIHPSEDDASNWQTMAQITDERVQLSDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSPPPAPTNLRISNLTTNNEGTVSVRITWDLPEEPDIPVHHYKVFW----SWTANRKSVVPAKKKRRKTTEGSQNSVALDGLQANSNYMVELQAITYWGQVRLKSGKVSLYFSTS--PPSTSLDQISK----ISKMKTDILAPPQRKRTARMLEIGAPFYQDNQLQVKVYWKKTEDSMLGLYHVQWFPESCAHNET---------------------KGPEKADHHIVLPALLFSCKYKVTVHPARPK-------------DHLKAESIFFTTPPCSTLKGKTHKQ---INCPSDTAPVVSKVLAKPENLSATFNVQEANITGYFS-WQLSKVN--LHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYMLTVHNLRPSMLYKLEVQVL 637          
BLAST of Anosmin-1 vs. Ensembl Xenopus
Match: psmc4 (proteasome 26S subunit, ATPase 4 [Source:Xenbase;Acc:XB-GENE-5855370])

HSP 1 Score: 157.147 bits (396), Expect = 3.957e-39
Identity = 160/679 (23.56%), Postives = 276/679 (40.65%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR----IKISEPYYHKNSLKIDLFITNSKKNL--VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPIL-----EPPN-KILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            D  + + ++ +A C  +CL +           +  +   H        QC +F+    + C   C+ +    L   +C N C+P        CL  C+FL + + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P    T  E + G   ++ W    + F+  + P++YV+Q R  +        ASNW+T+     E V L D++P  WYQFRVA+V  +GT GF + S   + +K P+ P  P NL          G +++R+ W  P   DIPV  YK+ W     +T      + A     +T  G+Q S  ++GL A+  Y V+++AI  WG   L S   +++ +T               ++    D+   +  PQ  R    ++I  P+Y  N L++ ++   ++ ++  +Y + + P  C   E+                    E+ +T    +  L  L FSC+Y + V P R +             D     +  F TP C  L  +   Q   + C +   P++     +P N    F     N   +   W+ ++ +  L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   41 DESLSAQSIYRARCTSRCLSLHIT--------RISAYFKHFQVQPWASQCSRFD----SRCLEPCKETWD--LRKNNCHNLCEPLFPKKHYECLTSCEFLKSVLSVKQGDCPAPEKASGFAAACVESCDADNEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELTFDEPQPGH-LDIKW---SSKFNISVEPVIYVVQRRWNYGIHPSEDDASNWQTMAQITDERVQLSDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSPPPAPTNLRISNLTTNNEGTVSVRITWDLPEEPDIPVHHYKVFW----SWTANRKSVVPAKKKRRKTTEGSQNSVALDGLQANSNYMVELQAITYWGQVRLKSGKVSLYFSTTL-------------NNVFLKDKTDILAPPQRKRTARMLEIGAPFYQDNQLQVKVYWKKTEDSMLGLYHVQWFPESCAHNETKG-----------------PEKAVTQDH-HIVLPALLFSCKYKVTVHPARPK-------------DHLKAESIFFTTPPCSTLKGKTHKQ---INCPSDTAPVVSKVLAKPENLSATFNVQEANITGYFS-WQLSKVN--LHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYMLTVHNLRPSMLYKLEVQVL 631          
BLAST of Anosmin-1 vs. Ensembl Xenopus
Match: psmc4 (proteasome 26S subunit, ATPase 4 [Source:Xenbase;Acc:XB-GENE-5855370])

HSP 1 Score: 157.147 bits (396), Expect = 5.188e-39
Identity = 165/679 (24.30%), Postives = 282/679 (41.53%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR----IKISEPYYHKNSLKIDLFITNSKKNL--VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPIL-----EPPN-KILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            D  + + ++ +A C  +CL +         +  Q++       S+I  Q    N +  + C   C+ +    L   +C N C+P        CL  C+FL + + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P    T  E + G   ++ W    + F+  + P++YV+Q R  +        ASNW+T+     E V L D++P  WYQFRVA+V  +GT GF + S   + +K P+ P  P NL          G +++R+ W  P   DIPV  YK+ W     +T      + A     +T  G+Q S  ++GL A+  Y V+++AI  WG   L S   +++ +T   P   S   +SK    +S  +   +  PQ  R    ++I  P+Y  N L++ ++   ++ ++  +Y + + P  C   E+                    E+ +T    +  L  L FSC+Y + V P R +             D     +  F TP C  L  +   Q   + C +   P++     +P N    F     N   +   W+ ++ +  L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   41 DESLSAQSIYRARCTSRCLSLHITRISAYFKHFQNN------GSLIWCQ----NHKQCSKCLEPCKETWD--LRKNNCHNLCEPLFPKKHYECLTSCEFLKSVLSVKQGDCPAPEKASGFAAACVESCDADNEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELTFDEPQPGH-LDIKW---SSKFNISVEPVIYVVQRRWNYGIHPSEDDASNWQTMAQITDERVQLSDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSPPPAPTNLRISNLTTNNEGTVSVRITWDLPEEPDIPVHHYKVFW----SWTANRKSVVPAKKKRRKTTEGSQNSVALDGLQANSNYMVELQAITYWGQVRLKSGKVSLYFSTS--PPSTSLDQISK----ISKMKTDILAPPQRKRTARMLEIGAPFYQDNQLQVKVYWKKTEDSMLGLYHVQWFPESCAHNETKG-----------------PEKAVTQDH-HIVLPALLFSCKYKVTVHPARPK-------------DHLKAESIFFTTPPCSTLKGKTHKQ---INCPSDTAPVVSKVLAKPENLSATFNVQEANITGYFS-WQLSKVN--LHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYMLTVHNLRPSMLYKLEVQVL 640          
BLAST of Anosmin-1 vs. Ensembl Xenopus
Match: psmc4 (proteasome 26S subunit, ATPase 4 [Source:Xenbase;Acc:XB-GENE-5855370])

HSP 1 Score: 156.762 bits (395), Expect = 6.781e-39
Identity = 153/607 (25.21%), Postives = 255/607 (42.01%), Query Frame = 2
Query:  524 LGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR----IKISEPYYHKNSLKIDLFITNSKKNL--VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPIL-----EPPN-KILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            L   +C N C+P        CL  C+FL + + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P    T  E + G   ++ W    + F+  + P++YV+Q R  +        ASNW+T+     E V L D++P  WYQFRVA+V  +GT GF + S   + +K P+ P  P NL          G +++R+ W  P   DIPV  YK+ W     +T      + A     +T  G+Q S  ++GL A+  Y V+++AI  WG   L S   +++ +T   P   S   +SK    +S  +   +  PQ  R    ++I  P+Y  N L++ ++   ++ ++  +Y + + P  C   E+                    E+ +T    +  L  L FSC+Y + V P R +             D     +  F TP C  L  +   Q   + C +   P++     +P N    F     N   +   W+ ++ +  L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   85 LRKNNCHNLCEPLFPKKHYECLTSCEFLKSVLSVKQGDCPAPEKASGFAAACVESCDADNEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELTFDEPQPGH-LDIKW---SSKFNISVEPVIYVVQRRWNYGIHPSEDDASNWQTMAQITDERVQLSDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSPPPAPTNLRISNLTTNNEGTVSVRITWDLPEEPDIPVHHYKVFW----SWTANRKSVVPAKKKRRKTTEGSQNSVALDGLQANSNYMVELQAITYWGQVRLKSGKVSLYFSTS--PPSTSLDQISK----ISKMKTDILAPPQRKRTARMLEIGAPFYQDNQLQVKVYWKKTEDSMLGLYHVQWFPESCAHNETKG-----------------PEKAVTQDH-HIVLPALLFSCKYKVTVHPARPK-------------DHLKAESIFFTTPPCSTLKGKTHKQ---INCPSDTAPVVSKVLAKPENLSATFNVQEANITGYFS-WQLSKVN--LHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYMLTVHNLRPSMLYKLEVQVL 624          
BLAST of Anosmin-1 vs. UniProt/SwissProt
Match: sp|P23352|KALM_HUMAN (Anosmin-1 OS=Homo sapiens OX=9606 GN=ANOS1 PE=1 SV=3)

HSP 1 Score: 151.369 bits (381), Expect = 2.321e-36
Identity = 166/675 (24.59%), Postives = 278/675 (41.19%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKI--SWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV--YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLV-------FWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            D  + + +V +A C  +CL +Q        Q  Q++       S++  Q    N +  + C   C+ SG   L    C + C+P     S  CL  C+FL   + +  G+CP   +           C +D +C     V+KCC+      C VP+   K G  L P   +   +  S ++  K+  + F+  + P++YV+Q R  +        A++W+T+  T  E V L D++P  WYQFRVA+V  +GT GF + S   + +K P++P  P NL      + ++G + + + W  P   DIPV  YK+  SW+       P  +     TD      G Q S  +E L  D  Y V+++AI  WG   L S   ++  T+        E+LV      +          P    +++  P+Y    L++ ++   ++   V  Y + + P  C    ++       + S    +E Y             L  L FSC+Y + VQP  IRP      + V            F TP C  L  +       + CL  A  +L   +K+L +  + +  SF+V        + +      L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +T Y ++VQ +
Sbjct:   36 DESLSAGSVQRARCASRCLSLQITRISAFFQHFQNN------GSLVWCQ----NHKQCSKCLEPCKESGD--LRKHQCQSFCEPLFPKKSYECLTSCEFLKYILLVKQGDCPAPEKASGFAAACVESCEVDNEC---SGVKKCCSNGCGHTCQVPKTLYK-GVPLKPRKELRFTELQSGQLEVKWS-SKFNISIEPVIYVVQRRWNYGIHPSEDDATHWQTVAQTTDERVQLTDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSAPPAPANLRLANSTVNSDGSVTVTIVWDLPEEPDIPVHHYKVFWSWMVSSKSLVPTKKKRRKTTD------GFQNSVILEKLQPDCDYVVELQAITYWGQTRLKSAKVSLHFTSTHA-TNNKEQLVKTRKGGIQTQLPFQRRRPTRP-LEVGAPFYQDGQLQVKVYWKKTEDPTVNRYHVRWFPEACAHNRTTG-----SEASSGMTHENY-----------IILQDLSFSCKYKVTVQP--IRPKSHSKAEAV-----------FFTTPPCSALKGKSH---KPVGCLGEAGHVL---SKVLAKPENLS-ASFIVQDVNITGHFSWKMAKANLYQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPSDHYVLTVPNLRPSTLYRLEVQVL 637          
BLAST of Anosmin-1 vs. UniProt/SwissProt
Match: sp|P33005|KALM_CHICK (Anosmin-1 OS=Gallus gallus OX=9031 GN=ANOS1 PE=2 SV=2)

HSP 1 Score: 144.05 bits (362), Expect = 5.265e-34
Identity = 159/670 (23.73%), Postives = 279/670 (41.64%), Query Frame = 2
Query:  329 AVCKASCYHKCLQMQANS-SQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTK--ASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHG-FTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR-IKISEPYYHKNSLKIDLFITNSKKNL-VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLV-------FWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            A   A C  +CL +Q    S      Q + ++A        +QC K        C   C+ S    L    C + C+P     +  CL  C+FL   + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P   ++  +  S ++  K+  + F+  + P++YV+Q R  +N+    ++  A+NW+T+  T  E V L D++   WYQFRVA+V  +GT GF + S   + +K P++P  P N+         +G +N+ + W  P   DIPV  YK+ W   +  +  P  +    +TD      G Q    +EGL  +  Y V+++A+ +WG   L S   ++  +T Q     +E+  +        D        +  R +KI  P+Y  N L++ ++   +  N+  +++      C+  ++  L           + E+  E  +        L  L FSC+Y + V P +   SK + K +          +  F TPSC     +      ++ C     P+L    K+L +  + +  SF+V        + +     +L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   37 AFSTARCTSRCLSLQITRISAFFKHFQNNGSLAWCQN---HKQCSK--------CLEPCKESWD--LKKNHCQSFCEPLFPKKNYECLTSCEFLKYILSVKQGDCPAPEKASGFAAACVESCEADSEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELKFIELQSGDLEVKWS-SKFNISIEPVIYVVQRR--WNQGIHPSEDDATNWQTVAQTTDERVQLSDIRASRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSAPPAPSNIRIANISANNDGTVNVMITWDLPEEPDIPVHHYKVFWSWTYSKYVIPAKKKRRKITD------GPQNYVVLEGLQPNSNYNVELQAVTRWGQIRLKSAKVSLHFSTAQDNRNNNEQTSAGKPPKGLVDPYPTFQRRKPTRFLKIGTPFYQDNQLQVKVYWKKTDINMNQFQVHSLLESCVHNDTKGL---------EKVTELTYENYM-------ILKDLSFSCKYKVTVLPAK---SKSRFKAE----------SIFFVTPSCSAFKEKTH---KHINCAAEEVPVLP---KVLAKPENLS-ASFIVQEGNITGHFSWKISKAVLHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYVLTVPNLRPSMLYRLEVQVL 632          
BLAST of Anosmin-1 vs. UniProt/SwissProt
Match: sp|Q90369|KALM_COTJA (Anosmin-1 OS=Coturnix japonica OX=93934 GN=ANOS1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 6.686e-33
Identity = 149/627 (23.76%), Postives = 264/627 (42.11%), Query Frame = 2
Query:  455 CKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTK--ASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHG-FTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGR-IKISEPYYHKNSLKIDLFITNSKKNL-VYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLV-------FWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            C+ + Q   + C   C+ S    L    C + C+P     +  CL  C+FL   + +  G+CP   +           C  D +C     V+KCC+      C VP+   K G  L P   ++  +  S ++  K+  + F+  + P++YV+Q R  +N+    ++  A+NW+T+  T  E V L D++   WYQFRVA+V  +GT GF + S   + +K P++P  P N+         +G +N+ + W  P   DIPV  YK+ W   +  +  P  +    +TD      G Q    +EGL  +  Y V+++A+ +WG   L S   ++  +T Q     +E+   +       D        +  R +KI  P+Y  N L++ ++   S  N+  +++      C   ++  L           + E+  E  +        L  L FSC+Y + V P +   SK + K +          +  F TP C     +      ++ C     P+L    K+L +  + +  SF+V        + +     +L  P   F+V W     +            Q  LP   +SQ++  +L  +   + + NL+ +  Y ++VQ +
Sbjct:   71 CQNYKQ--CSKCLEPCKESWD--LKRNHCQSFCEPLFPKKNYECLTSCEFLKYILSVKQGDCPAPEKASGFAAACFESCEADSEC---SGVKKCCSNGCGHTCQVPKNLYK-GVPLKPRKELKFIELQSGDLEVKWS-SKFNISIEPVIYVVQRR--WNQGIHPSEDDATNWQTVAQTTDERVQLSDIRASRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSAPPAPSNIRIANISANNDGTVNVMITWDLPEEPDIPVHHYKVFWSWTYSKYVIPAKKKRRKITD------GPQNYVVLEGLQPNSNYNVELQAVTRWGQIRLKSAKVSLHFSTTQDNRNNNEQTSVEKPPKGVVDPYPTFQRRKPTRFLKIGTPFYQDNQLQVKIYWKKSGINMNQFQVHSLLESCTHNDTKGL---------EKVTELTYENYM-------ILKDLSFSCKYKVTVLPAK---SKSRFKAE----------SIFFVTPPCSTFKEKTH---KHINCAAEEVPVLP---KVLAKPENLS-ASFIVQEGNITGHFSWKISKAVLHQPMTGFQVTWAEVTTES----------RQNSLPNSIISQSQ--ILPADHYVLTVPNLRPSMLYRLEVQVL 632          
BLAST of Anosmin-1 vs. TrEMBL
Match: A0A4E0RJ34 (Anosmin-1 OS=Fasciola hepatica OX=6192 GN=D915_003272 PE=4 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 6.127e-67
Identity = 201/726 (27.69%), Postives = 334/726 (46.01%), Query Frame = 2
Query:  338 KASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTS-CTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDK--------------LLDRECHLDQQCMIEGDVRKCC----NLRCVVPQFHPKIGP---SLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGF--KSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQL-PV--RESE-----RLVSKDSSSLSGDQCSC-VDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPL-RIRPSKRKIKKDVNLD-------------DEQFVLTFCFCTPSCLKL--------------ISQPQYQSTNLRCLNRANP----ILEPPNKILFQRSHKNPESFLVFWEYTEK--SDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIF-----YQQEMEQIS 2296
            +A C  KCL++  ++++    + + + +  S    +  +C + N E  +S C T C+         K+C + C     GC  GC  L  ++    G CP   ++ K                 +EC LD  C     + KCC    N  C  P F+  + P   SL P   +     +   + W    +  ++   PI+++LQ R    +  + T  S+W+TL++T + G  L   +PG  YQFR+A+V+ +GT GF  ++ + P +  + P+ P  PRN+TD  WR  ++G I++ + W  P   D+P+TEY I+WV DHG+ Q  G SLE LT F Q V  ++  Y I+ L    +YKVQV A+  W G   L S PNTIFL+T  + PV  R+S      R+  +   +     C C  D P     ++ + +Y + SL      T   K   + + ++P VCI+++             +DI  + +   +++     TLG L F+CRY +++  + R R S+ +   D + D             D   V   CFCTPSCL++                 P      L     ++P    I   P K+   +S     S L   ++T    S+   L   ++RVIW P +++ V+++     +     P +D  Q++ ++++EN + I L +L+  TFYIV+VQ +      YQ+E   ++
Sbjct:   43 RAQCKAKCLRLFQSANE--QDLFKPTWVQTSAQWSLLSKCFRNNSEHCSSECFTACDGPP------KECLSSCSTQGIGCRIGCHHLQESVDPRPGQCPRPADMPKPALKRSPTKLTETMRCGKECRLDSSC--SDSLHKCCSIGCNRVCTQPTFNDSVPPMPDSLIPQ--ISEVSNAILALDWSAPYSQLTKICGPIVFILQTRICACKMLDATHLSDWQTLIMTHQFGAKLDAFEPGKMYQFRIAAVSPHGTRGFGPETEAYPSR-PEIPSVPSPPRNVTDSMWRFYSSGSISVLLSWQPPIRTDLPITEYVINWVVDHGYVQSGGHSLEGLTQFSQKVAPDRLQYVIQNLKPATSYKVQVIAVSYWHGHGQLKSQPNTIFLSTQSIHPVYTRQSTMEDGFRVSMRSFPTEQKSNCECGPDDPAQKLFRLEKTFYDRGSLMAKFLFTPPNKGTDHLVEWSPQVCINSKE------------NDILTLKQHILVSSRTRVVTLGNLSFNCRYMVKITSVDRQRQSRDRNWFDSSHDSSPLTAISPFDDHDSVSVKRSCFCTPSCLEVEVAEGPAPINCAPTDPGPPLPPAGLTAAQTSSPLSYRIQWNPPKVDETQSETPTSSTLANRQHTRAALSNQSPLLGTKYRVIWAPRVEEPVNKEMYNDDVG--FSPIMDTQQSDVRIVEENQTWIELRDLQSGTFYIVRVQTLITSETGYQRESSPVA 741          
BLAST of Anosmin-1 vs. TrEMBL
Match: A0A504YS35 (Anosmin-1 OS=Fasciola gigantica OX=46835 GN=FGIG_03019 PE=4 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 6.943e-66
Identity = 201/730 (27.53%), Postives = 325/730 (44.52%), Query Frame = 2
Query:  338 KASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTS-CTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDK--------------LLDRECHLDQQCMIEGDVRKCC----NLRCVVPQFHPKIGP---SLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGF--KSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQL-PV--RESE-----RLVSKDSSSLSGDQCSC-VDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVN----------------LDDEQFVLT--FCFCTPSCLKLISQPQYQSTN------LRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILL--------------LPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIF-----YQQEMEQIS 2296
            +A C  KCL++  ++++    + + + I  S    +  +C + N E  +S C T C+         K+C + C     GC  GC  L  ++    G CP   ++ K                 +EC LD  C     + KCC    N  C  P F+  + P   SL P   +     +   + W    +  ++   PI+++LQ R    +  + T+ S+W+TL++T + G  L   +PG  YQFR+A+V+ +GT GF  ++ + P +  + P+ P  PRN+TD  WR  ++G I++ + W  P   D+P+TEY I+WV DHG+ Q  G SLE LT F Q V  ++  Y I+ L    +YKVQV A+  W G   L S PNTIFL+T  + PV  R+S      R+  +   +     C C +D P     ++ + +Y + SL          K   + + ++P+VCI+++      ND+      I    R R +       TLG L F+CRY +++  +      ++   D N                 DD   V     CFCTPSCL++         N            A       +  L  R   NP  F      T  S  L               L   ++RVIW P +++ V+++     +     P +D  Q++ ++++EN + I L +L+  TFYIV+VQ +      YQ+E   ++
Sbjct:   43 RAQCKAKCLRLFQSANE--QDLFKPTWIQTSAQWSLLSKCFRNNSEHCSSECFTACDGPP------KECISSCSTQGIGCRIGCHHLQESVDPRPGQCPRPADMPKPALKRSPTKLTETMRCGKECRLDSSC--SDSLHKCCSIGCNRVCTQPTFNDSVPPMPDSLIPQ--ISEVSNAILALDWSAPYSQLTKICGPIVFILQTRLCACKMLDATQLSDWQTLIMTHQFGAKLDAFEPGKMYQFRIAAVSPHGTRGFGPETEAYPSR-PEIPSVPSPPRNVTDSMWRFYSSGSISVLLSWQPPIRTDLPITEYVINWVVDHGYVQSGGHSLEGLTQFSQKVAPDRLQYVIQNLKPATSYKVQVIAVSYWHGHGQLKSQPNTIFLSTQSIHPVYTRQSTMEDRFRVSIRSFPTEQKSNCECGLDDPAQKLFRLEKTFYDRGSLMAKFLFKPPNKGTDHLVEWSPHVCINSKE-----NDILTLKQHILVSSRTRAV-------TLGNLSFNCRYMVKITSV----DGQRQSGDRNWFDSSHDSSPLTAMSPFDDHDLVSVKRSCFCTPSCLEVEVAEGPAPINCSPTDPGPPPPPAGLTAAQTSSPLSYRIQWNPPKFDETQSETLTSSTLANRQRTRAALSNQSPLLGTKYRVIWAPRVEEPVNKEMYNDDVG--FSPIMDTQQSDVRIVEENQTWIELRDLQSGTFYIVRVQTLITSETGYQRESSPVA 741          
BLAST of Anosmin-1 vs. TrEMBL
Match: A0A095C8H7 (Anosmin-1 (Fragment) OS=Schistosoma haematobium OX=6185 GN=MS3_06907 PE=4 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 1.686e-64
Identity = 190/647 (29.37%), Postives = 307/647 (47.45%), Query Frame = 2
Query:  479 TTSCTTGCEYSGSSGLGGKDCSNQCQPNS-SGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHP-KIGPSLWPP------TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFK-SFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQL----------PVR-----ESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNS-KKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSK------RKIKKDVNLDDEQF---VLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFW---------------EYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIF 2269
            T  C  GC       L  + C NQ  PN  S  +     LN    ++  N  +  E+   +  +C     C +  D + CC  +C   Q +   I   L PP      + +   + +  +++W     + +  M PI+++LQ+R    + F+   AS W+TL++T   G NL+  +PG  YQFR+A+V+ +GT GF  S     +    P+ PG PRN+TD  WR+ ++GKI++ + W  P   D+P+TEY I+W  DHG+   DGQ+LEAL  F QT+   +    ++ L    +YK+QVRAI  W G   L S PNTIFL+T  +          P++      + +L ++ +       C C DGPQ   +K  + +Y  ++LK  L I  + K      I + P VCID  S++L ++ L   PS I  + + +     L N     L F+CRY ++V   R+ P+         ++  V++ ++QF   V   CFCTPSC  ++ +      N  C+  A     PP K+  Q  H +  ++ + W                ++   + L   + ++R++W P I + VD       I     P +D+ Q + +V+ ++ + I L  LK NT YIV VQ + 
Sbjct:    2 TIGCQIGC------FLIKQTCENQ--PNECSNSMYLMNDLNYTSSINQNNQTQYNELKYPVVNDC--VNNCQLHNDCKTCCKSKC--HQLNTDSIYNELVPPRPIHLNSTISSTNTNIIKLSWSVQYQNITSTMNPIVFILQIRICICKVFDENYASTWQTLIMTHEFGANLEAFEPGRLYQFRIAAVSIHGTKGFGLSTDAYPRDPIRPSPPGSPRNVTDSMWRLYSSGKISLLLKWQPPVRSDLPLTEYLITWSIDHGYLHTDGQTLEALIQFTQTINAERTECILDNLKPATSYKIQVRAISYWHGFGNLESQPNTIFLSTQSIHPAFHQQIVNPIQIYTTPNTIKLPTEFTFKSKSSNCECGDGPQPLVLK--KIFYENHNLKAVLQINMTIKTENTTIIQWYPQVCID--SNNLILDTL---PSTI--LLKSKDKRVILEN-----LHFNCRYDVRV---RVYPANLDAKYITMVESVVHVQNQQFDVIVYVGCFCTPSCSNVLKKEGRLPDN--CILPALGPPSPPTKV--QVIHISDLNYRISWIQPMTTSDRNVNEHSSFSSSVNKLNKNSTKYRIMWAPRIKEPVDESMYNDKIG--FSPIMDLQQIDVRVIDKDQTWIILNQLKPNTLYIVHVQTLL 613          
BLAST of Anosmin-1 vs. TrEMBL
Match: A0A094ZMF2 (Anosmin-1 OS=Schistosoma haematobium OX=6185 GN=MS3_03459 PE=4 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 2.401e-63
Identity = 220/783 (28.10%), Postives = 339/783 (43.30%), Query Frame = 2
Query:  500 CEYSGSSGLGGKDCSNQCQ----PNSSGCLEGCQFLNTAIKMSLGNCPE-------------------------------KFEIDK--------------LLDRE--------------CHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAK-TPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDS--SSLSGDQCSCVDGPQDGRI------KISEPYYHKNSLKIDL-FITN-SKKNLVYK--------------------------------------ISYAPNVCIDT--------------ESSSLFVNDL----QPSPSDI---------------------------NEIYRERGITAALPNAT--LGPLQFSCRYTLQVQPLRIRPSKRKIKK--DVNLDDEQFVLTFCFCTPSC--LKL---ISQPQYQSTNLRCLNRA---NPILEPPNKILFQRSHKNPESFLVFWE-------------------YTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEM 2284
            CE +  S     DC   C+    P+   CL GC+ L+ A+K   G+CPE                               +F +DK              +L+                C +D +C  + D  KCC  +C    F+  I P +    I+E K   SF ++W  ++   S   LP +YVLQV++YF  +++   +S WKTL+++  +G  L D   G WYQF+VA+V ++G+ GF + SPPV+L    P+ P  PR+L D    ++ANGKI +++ W  PA   +PVTEY+ISW  D      + +  E  T FV TVP +Q +Y +  L     Y+++V A+ +WGS  L S PNT+F+ TP +   +  R   +DS  ++L     + V   ++ +         SE +   NS K D+ F+ N + K+LV+K                                      + + P VCI+T              +S +L  N++    +PS S I                           N +  +R     L +AT  L  L F+CRY +++   +   SKR  KK   V        L+   CTP+C  +KL   I +P       +CL      + I EP N    Q S     S+ + W+                   Y++K+  ++ P V  R+IWGPS D  +     +   N  L PRID ++AETKVL+ + +SI L+NL  NT Y+V +QA+  +  M
Sbjct:   24 CEQACDSDRFYSDCIKMCESGDSPSGWSCLTGCRSLDAALKRRSGDCPEQIGLAIHRIPNLVSVKNDVQNYPSHESSESSQFNLDKPISLPESDNGLKTNMLENSWFTHHSSAINEITSCTVDAECPQKQD--KCCQSQCKQALFNKAILPPIPTVRILEEKHPPSFLVSWNNNEIEVSNLSLPTVYVLQVKSYFGPEYDNRMSSPWKTLVVSTLKGAQLSDPAVGWWYQFQVAAVNQWGSLGFDTPSPPVQLTTLQPSPPSPPRDLADELLTLQANGKIRVQIQWNSPATTVLPVTEYRISWGPDSANIDNNHKFTEYPTQFVHTVPSSQTNYQLTNLYPGVLYRIEVIAMSEWGSTQLRSQPNTLFIHTPNVDSIQDHR---EDSLIANLKKIDANTVQHLKNKKYLNHYDESYSEDHLTHNSGKSDVSFVCNGTSKSLVFKSSLPSDSSINHINIKTAELSVETDVAVFDGHVSSRPLIVRWTPQVCIETVIVTGNNIDETNSFQSEALIANNIDQFTKPSYSQIENAGRRKESLNSNEKYILLNPVKHGVNNHVDGKRFKKPILRHATLQLTNLNFNCRYLIELLSEKQESSKRSDKKIHPVYTSGYNVELSAWLCTPACSTVKLASWIKKP-------KCLGTTQVLDIITEPKNLSYKQISVTPTISYNISWKSGALVSRSGSYQQLTTQEIYSDKTSKIIFP-VHHRIIWGPSRDTAMKSVVWKSWPN--LRPRIDHTRAETKVLRNSETSIVLDNLLPNTLYVVSLQAMTGEHYM 791          
BLAST of Anosmin-1 vs. TrEMBL
Match: A0A430QBF1 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0001289 PE=4 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 5.113e-63
Identity = 205/702 (29.20%), Postives = 324/702 (46.15%), Query Frame = 2
Query:  338 KASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNS-SGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHP-KIGPSLWPP------TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFK-SFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQL----------PVR-----ESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNS-KKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRK------IKKDVNLDDEQF---VLTFCFCTPSCLKLISQPQYQSTNL---RCLNRANPIL-----EPPNKILFQRSHKNPESFLVFW---------EYTEKSDI------LLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIF 2269
            +A C  KCL++  N   PG Q                   K F+ + T  C  GC       L  + C NQ  PN  S  +     LN    ++  N  +  E+   +  +C     C +  D + CC  +C   Q +   I   L PP      + +   + +  +++W     + +  M PI+++LQ+R    + F+   AS W+TL++T   G NL+  +PG  YQFR+A+V+ +GT GF  S     +    P+ PG PRN+TD  WR+ ++GKI++ + W  P   D+P+TEY I+W  DHG+   DGQ+LEAL  F QT+   +    ++ L    +YK+QVRAI  W G   L S PNTIFL+T  +          P++      + +L ++ +       C C DGPQ   +K  + +Y  ++LK  L I  + K      I + P VCID  S++L ++ L   PS I  + + +     L N     L F+CRY ++V   R+ P+         ++  V++ ++QF   V   CFCTPSC  +I +      N         A+ IL      PP K+  Q  H +  ++ + W            E S +      L   + ++R++W P I + VD       I     P +D+ Q + +V+ ++ + I L  LK NT YIV VQ + 
Sbjct:   49 RARCKAKCLRLFNN---PGEQ-------------------KAFSDK-TIGCQIGC------FLIKQTCENQ--PNECSNSMYLMNDLNYTSSINQNNQTQYNELKYPVVNDC--VNNCQLHNDCKTCCKSKC--HQLNTDSIYNELVPPRPIHLNSTISSTNTNIIKLSWSVQYQNITSTMNPIVFILQIRICICKVFDENYASTWQTLIMTHEFGANLEAFEPGRLYQFRIAAVSIHGTKGFGLSTDAYPRDPIRPSPPGSPRNVTDSMWRLYSSGKISLLLKWQPPVRSDLPLTEYLITWSIDHGYLHTDGQTLEALIQFTQTINAERTECILDNLKPATSYKIQVRAISYWHGFGNLESQPNTIFLSTQSIHPAFHQQIVNPIQIYTTPNTIKLPTEFTFKSKSSNCECGDGPQPLVLK--KIFYENHNLKAVLQINMTIKTENTTIIQWYPQVCID--SNNLILDTL---PSTI--LLKSKDKRVILEN-----LHFNCRYDVRV---RVYPANLDAKYVTLVESVVHVQNQQFDVIVYVGCFCTPSCSNVIKKEGRLPDNCILPEMTVYAHIILALGPPSPPTKV--QVIHISDLNYRISWIQPMTTSDRNVNEHSSLSSSVNKLNKNSTKYRIMWAPRIKEPVDESMYNDEIG--FSPIMDLQQIDVRVIDKDQTWIILNQLKPNTLYIVHVQTLL 694          
BLAST of Anosmin-1 vs. Ensembl Cavefish
Match: anos1a (anosmin-1-like [Source:NCBI gene;Acc:103026549])

HSP 1 Score: 130.568 bits (327), Expect = 1.083e-30
Identity = 121/470 (25.74%), Postives = 190/470 (40.43%), Query Frame = 2
Query:  536 DCSNQCQ---PNSSG-CLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPK---IGPSLWP--PTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISW---VRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV-YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKL 1897
             C   C+   P   G C+  C+FL + + +  G CP               C  D +C      +KCC   C      P+    G  L P    + E       E+ W    + F+    P+L VLQ R  +        A+ W+ +  T  E V L D++PG WYQFRVA+V  +GT G+ + S   + +K PA P  P  L         +  +++ V W  PA  D+PV  YK+SW   V     + P            +T+ G+  S ++EGL  +++Y ++++A+  WG   L S+  T+  TT       SE   +  SS     Q   +D        +  P+Y    L++ ++       +  Y++ + P  C                 S I E    +   A+LP      LQFSC Y + +QP     SK +++ +  +          F TPSC  L
Sbjct:   90 SCRELCEHTFPKRHGECVTSCEFLRSVMVVKQGECPAPERAAGFAAACVEGCEEDGECSSH---KKCCPNGCGHTCQSPRNLFRGAPLKPRKELLFEELPTGVLEVRW---SSKFNVSAEPVLNVLQRRWNYGIHPSEDGATEWQVVAQTSEERVWLSDVRPGRWYQFRVAAVNVHGTRGYTTPSRHFRSSKDPAPPPAPSELQVKHLSFGPDRSVSVHVSWAAPADLDVPVHHYKLSWSWSVSGAELSTPPKLKRR------KTINGDSTSAELEGLRENRSYTLELQAVSYWGQLPLKSSKTTLRFTT-----YRSEPQPTVSSSVPQKSQSDVLD--------VGTPFYQDGQLQVRVYWKKRDPTVSRYRVHWFPEFCSHNG-------------SRIQEKLITQDNYASLPG-----LQFSCTYKVIIQPAG---SKGRVQAESTV----------FYTPSCASL 503          
BLAST of Anosmin-1 vs. Ensembl Cavefish
Match: anos1b (anosmin-1 [Source:NCBI gene;Acc:103026226])

HSP 1 Score: 123.635 bits (309), Expect = 1.742e-28
Identity = 127/505 (25.15%), Postives = 199/505 (39.41%), Query Frame = 2
Query:  341 ASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCE--YSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLL---------DRECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFT-QPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLF----ITNSKK-NLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPL 1795
            A C+ +CL +           Q  ST  H  +  +   C+  N    + C   C+  +     L  K C    +     C+   +FL +      G+CP                  DREC   ++C   G     C   C  P    K G  L P    T +E + G   E+ W    + F+  + P+LY+LQ R           AS W T+L+T  E   L+D++P  WYQFRV++V   GT GF + S      + P  P  P+        + A+G +++ + W  PA  D+ V  YK++W    G    P+G+    +TD      G      + GL  D  Y VQ++A+   G   L S      L+        +    S  S   S +       P   R++ + P+YH N L++ +F    ++ S+K +  Y +S+ P VC                    N   RE+  T    +  +  L F+C+Y + V PL
Sbjct:   45 ARCFSRCLTLHIT--------QLTSTFQHLQSDEVLGWCE--NHRRCSQCLQPCKELWETRKALSHKLCEKHHE-----CVTSAEFLLSLQSQKQGDCPAPQRATGFAAACVESCSSDRECSASKKCCSNG-----CGHTCQPPANLFK-GVPLKPRKDMTFIEDQYGQ-LEVAWM---SKFNVSIEPVLYILQRRWNDGIHPSEDDASTWYTVLMTMEERAVLKDIRPQRWYQFRVSAVNSQGTRGFTTPSKHFFSTRDPLPPETPQKARVENETVHADGSVSVLLRWDPPAEGDLVVDRYKVTWSARGGLAGGPNGKESGRVTD------GTLTEMQLGGLQPDTRYTVQIQAVSFSGQKRLKS--GRAQLSFTTAAAAAASTGSSPPSREFSNELPISRSSPSVLRLEAAAPHYHDNQLQVKVFWKSRVSESQKASSSYLLSWYPEVC------------------GGNVTKREKMATVQGTHFIITGLLFACKYKVAVMPL 498          
BLAST of Anosmin-1 vs. Ensembl Cavefish
Match: ANOS1 (anosmin 1 [Source:HGNC Symbol;Acc:HGNC:6211])

HSP 1 Score: 116.316 bits (290), Expect = 3.000e-26
Identity = 152/624 (24.36%), Postives = 249/624 (39.90%), Query Frame = 2
Query:  572 CLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCN----LRCVVPQFHPKIGPSLWP--PTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGF----KSFSPPVKLAKTPASPGVP-RNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTI-FLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV--YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANP--ILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKT--KLIILST--HKDPSFPKLSVSNHHQQ 2374
            CL  C+FL +      G+CP               C  D +C      RKCC+      C VP+   K  P L P    +   +     E+ W    + F+  + P++YV+Q R           A+ W+ +     E V L D +P  WYQFRVA+V  +GT GF    ++F  P+  +  P+  G+   N++ G      +G +  R+ W  P   D+PV  +K+ W     +T P    +       +T  G + S D+EGLV + +Y V+++A+  WG   L S   ++ F T P       E   SK S+ L  ++      P    +++  P+Y    L++ ++      +    + + +    C   E+S       QP  S   E Y             L  L FSC+Y + V  L+   +KR+ + D          +  F TPSC  L ++     T   C     P  + +P N      +H+   +    W  +       +    F+V W            +     Q  LP   +SQ++  +L  + + + + +L+  + Y ++VQ I    E     KT    +I ST  HK    PKL     HQ+
Sbjct:  112 CLTSCEFLRSVESAKQGDCPAPERASGFAAACVESCEEDAECPAH---RKCCSNGCGHTCQVPRNLYKGAP-LKPRKELVFVEQPAGPLEVRW---SSKFNISVEPVVYVVQRRWNHGIHPSEDDATPWQDVAQVAEERVQLVDTRPSRWYQFRVAAVNVHGTRGFTAPQQTF--PLDPSPPPSPSGLKVSNISSG-----KDGFLTARLDWTLPEESDVPVHHFKVFW----SWTVPGKALVPTKKKRRKTTSGAKSSVDLEGLVGNSSYTVELQAVAYWGQVRLKSPKASLQFSTAP-------ENNHSKISTKLKKEEFPAGKRPAS-MLEVGTPFYQDGQLQVRVYWKKRGDSSATRHHVQWTAEHCSHNETS-------QPEKSVTQENY-----------INLPGLLFSCKYRVSVYTLK---NKRRSEDD----------STTFITPSCSTLRTKTHKPIT---CPGEGAPKVLAKPENLTASFSTHEGNVTGNFHWRISHPQPQHTITG--FQVTWAE----------VTTTSRQNSLPNSIISQSQ--ILPPDHNFLVVPSLRPASSYRLEVQVITTAGEGPATMKTFHTPVIFSTLQHK----PKLRQHPQHQR 657          
BLAST of Anosmin-1 vs. Ensembl Nematostella
Match: EDO36668 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI12])

HSP 1 Score: 66.6254 bits (161), Expect = 1.491e-11
Identity = 67/279 (24.01%), Postives = 116/279 (41.58%), Query Frame = 2
Query:  662 RECHLDQQCMIEGDVRKCCN-LRCVVPQFHPKIG--PSLWPPTIMEG-----KDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKS----FSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW------GSAALISTPNTIFLTTPQLPVRES 1444
            R+   ++  ++    R  C  L+ +     P I    +L PPT+++      K+  S  ++W   + S  E     +YV+Q RT    +    +   W  L  T  + + + D    +W QFRVA V +  ++ F +    F PP +       PG  R+       I  N K  I + W +P   D+PV++Y++ W +       D Q L    +    VP  +    I GL  + TY + ++AI ++      G    + T   +FL     P+ +S
Sbjct:   67 RQLEANKDWLVNTACRGSCKFLKALFNNGKPSISNPANLQPPTVVKAFYTRTKNMRSLTVSW---NISAPESKDGWVYVVQQRTARQHELPQ-QMGKWVPLTQTRSQQLKIPDPGSALWCQFRVAVVQQDSSSNFTTSTLTFIPPER-------PGPVRDFRVTGKHILKN-KAQISIAWRKPENSDLPVSKYRVYWSKRLDTYSTDWQHLR---EHRGRVPATRHKAIIHGLELNTTYHIAIQAISRYQGKRLKGPTMELYTRTLVFLILKLAPLSKS 330          
BLAST of Anosmin-1 vs. Ensembl Medaka
Match: anos1 (anosmin 1 [Source:NCBI gene;Acc:100049494])

HSP 1 Score: 154.066 bits (388), Expect = 3.124e-38
Identity = 179/719 (24.90%), Postives = 295/719 (41.03%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPK---IGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVS-KDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV--YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANP----ILEPPNKILFQRSHKNPE---SFLVFWEYTEKSDILLLPNVR---FRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKT----KLIILSTHKDPSFPKLSVSNHHQQ 2374
            D    +A++ +A C  +CL +         +  Q++       S++  Q    N +  + C   C+ S    L    C + C+P        CL  C+FL +   +  G+CP   +           C  D +C     V+KCC+  C      PK    G  L P      +E   G   E+ W    + F+  + P+LYV+Q R  +        A+ W+T+  T  E + L D +   WYQFRVA+V  +GT GF + S   + ++ PA+P    NL      +  +G +  R++W  P   DIP+  YK+ W     +T P    + +     +T+ G Q   D+EGL  + +Y V+++AI  WG   L S  +++  TT Q        L S KD++S  G   S +     G +++  P+Y    L++ ++        V  Y + + P  C   E+         P  S   E Y             L  L FSC+Y + VQ L+   SKR+ K +          +  F TPSC  + S+      ++ C          +L  P  +    S K+     SFL  W  +      + P+ R   F+V W            +     Q  LP   +SQ++  +L  + + + + NL+ +TFY ++VQ I    E     KT     ++ ++ H+    P+L   + H Q
Sbjct:   31 DDSFFTASIYRARCASRCLSLHITRISAFFKHSQNN------GSLVWCQ----NHKQCSKCLEPCKESWD--LKENQCQDLCEPLFPKKHYECLTSCEFLKSVEGVKQGDCPAPEKASGFAAACVESCEEDGEC---SAVKKCCSNGCGHTCQLPKNLYKGVPLKPRKELVFLEHPSGQ-LEIHW---SSKFNISVEPVLYVVQRRWNYGIHPSEDDATEWQTVAQTAEERIQLADTRASRWYQFRVAAVNVHGTRGFTAPSKHFRSSRDPAAPPSAANLHVTNMSLADDGLVTARLNWTLPHEPDIPIHHYKVFW----SWTVPTKSMVPSKKKQRKTINGAQNWLDLEGLQQNSSYSVELQAITYWGQVRLKSPKSSLHFTTNQNNESAKSMLKSKKDATSPVG---STLAKRPSGALEVGTPFYQDGQLQVRVYWKKRGDPSVSRYHVQWMPEYCNHNETRG-------PEKSVTQENY-----------INLPGLLFSCKYKVTVQLLK---SKRRSKDE----------STTFLTPSCATIRSKTH---KHIPCPGEGAAGHPKVLAKPENLTASFSIKDGNITGSFL--WGMSR-----VAPHHRITGFQVTWAE----------INTESRQNSLPNSIISQSQ--ILPPDPNMLVVSNLRPSTFYRLEVQVITTGGEGPATVKTFQTPNILPVTQHR----PRLRQHHPHHQ 666          
BLAST of Anosmin-1 vs. Ensembl Medaka
Match: anos1 (anosmin 1 [Source:NCBI gene;Acc:100049494])

HSP 1 Score: 152.91 bits (385), Expect = 8.951e-38
Identity = 171/690 (24.78%), Postives = 281/690 (40.72%), Query Frame = 2
Query:  308 DHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQP----NSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCCNLRCVVPQFHPK---IGPSLWPP---TIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLV--YKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANP----ILEPPNKILFQRSHKNPE---SFLVFWEYTEKSDILLLPNVR---FRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKT 2302
            D    +A++ +A C  +CL +         +  Q++       S++  Q    N +  + C   C+ S    L    C + C+P        CL  C+FL +   +  G+CP   +           C  D +C     V+KCC+  C      PK    G  L P      +E   G   E+ W    + F+  + P+LYV+Q R  +        A+ W+T+  T  E + L D +   WYQFRVA+V  +GT GF + S   + ++ PA+P    NL      +  +G +  R++W  P   DIP+  YK+ W     +T P    + +     +T+ G Q   D+EGL  + +Y V+++AI  WG   L S  +++  TT Q    ES + + K     +    S +     G +++  P+Y    L++ ++        V  Y + + P  C   E+         P  S   E Y             L  L FSC+Y + VQ L+   SKR+ K +          +  F TPSC  + S+      ++ C          +L  P  +    S K+     SFL  W  +      + P+ R   F+V W            +     Q  LP   +SQ++  +L  + + + + NL+ +TFY ++VQ I    E     KT
Sbjct:   31 DDSFFTASIYRARCASRCLSLHITRISAFFKHSQNN------GSLVWCQ----NHKQCSKCLEPCKESWD--LKENQCQDLCEPLFPKKHYECLTSCEFLKSVEGVKQGDCPAPEKASGFAAACVESCEEDGEC---SAVKKCCSNGCGHTCQLPKNLYKGVPLKPRKELVFLEHPSGQ-LEIHW---SSKFNISVEPVLYVVQRRWNYGIHPSEDDATEWQTVAQTAEERIQLADTRASRWYQFRVAAVNVHGTRGFTAPSKHFRSSRDPAAPPSAANLHVTNMSLADDGLVTARLNWTLPHEPDIPIHHYKVFW----SWTVPTKSMVPSKKKQRKTINGAQNWLDLEGLQQNSSYSVELQAITYWGQVRLKSPKSSLHFTTNQ--NNESAKSMLKSKKDATSPVGSTLAKRPSGALEVGTPFYQDGQLQVRVYWKKRGDPSVSRYHVQWMPEYCNHNETRG-------PEKSVTQENY-----------INLPGLLFSCKYKVTVQLLK---SKRRSKDE----------STTFLTPSCATIRSKTH---KHIPCPGEGAAGHPKVLAKPENLTASFSIKDGNITGSFL--WGMSR-----VAPHHRITGFQVTWAE----------INTESRQNSLPNSIISQSQ--ILPPDPNMLVVSNLRPSTFYRLEVQVITTGGEGPATVKT 642          
BLAST of Anosmin-1 vs. Ensembl Medaka
Match: anos1b (anosmin 1b [Source:NCBI gene;Acc:100049297])

HSP 1 Score: 94.7449 bits (234), Expect = 2.172e-19
Identity = 125/549 (22.77%), Postives = 198/549 (36.07%), Query Frame = 2
Query:  341 ASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCE--YSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD---RECHLDQQCMIEGDVRKCC----NLRCVVPQFHPKIGPSLWPPTIMEGKDGSS--FEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANG----------------KINIRVHWMEPAAFDIPVTEYKISWVRDHGFT-QPDGQSLEA----LTDFVQTVP-------------------GNQRSYDIEGLVADKTYKVQVRAICKWGSAALIS-TPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDG----------RIKISEPYYHKNSLKIDLFIT-----NSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQV 1786
            A C  +CL +        +  Q  +   H  +  +   C+  N      C   CE  +   + L  K C  Q +     C+   +FL +      G+CP               C  DQ C      RKCC       C  P    K G  L P   M   + S    ++ W    + F+  M P++Y+L+ R           AS W  + +T  E V L DL+P  WYQFRVA+V   G+ GF + S     +K P+ P  P N+      +   G                 + + ++W  P+  D+ +  Y+I+W+  H  T      SL+A    L    Q  P                   G Q    ++GL+    Y + V+++  WG   L S     +F T P      S  L S  SS              D           R++ + P+YH N L++ ++         K   +Y + + P+ CI+  +S                  +E+  T    + T+  L F+C+Y + V
Sbjct:   37 ARCTSRCLSL--------HMTQITAAFRHIQSDEVLVWCE--NHRRCAQCLQPCEELWETRTVLPPKACEKQTE-----CMTSREFLASLRSSRQGDCPPPQRATGFAAACVESCSSDQHC---PSSRKCCFNGCGHTCQAPANLYK-GVPLKPRREMSFTEDSEGHVKVGWV---SKFNVSMEPVVYLLESRWNIGIHPSEDHASPWSAVAMTLSEEVLLTDLRPQRWYQFRVAAVNSQGSRGFTTPSKHYISSKDPSPPEPPMNIRVSNQTLNWAGLQLMPKVTERSSESGTTVAVLLNWEPPSEGDLLIRNYRITWMPLHVHTAHKHTHSLQANAQRLHKNKQKWPTNSHRQEQKKKESNTVLTAGAQNELWVQGLLPGTPYSLSVQSVAYWGQKRLKSHRAQLVFTTIPNADGDFSNELPSSSSSLSPSSPSYPSVSQPDSPVNPVPSEKLRLEAAAPHYHDNQLQVKVYWKWPHHFRPKHQDLYILRWHPHTCINNVTS------------------KEKMTTVQGTHHTIKGLHFACKYRVSV 545          
BLAST of Anosmin-1 vs. Planmine SMEST
Match: SMESG000010608.1 (SMESG000010608.1)

HSP 1 Score: 1534.24 bits (3971), Expect = 0.000e+0
Identity = 751/752 (99.87%), Postives = 752/752 (100.00%), Query Frame = 2
Query:  215 VISYSIVNGLTMSSDSFVRNKPFFDNSYINPDHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKTKLIILSTHKDPSFPKLSVSNHHQQLMAQGKANVRHGXXXXXXXXXXXXXXXWRLGT 2470
            +ISYSIVNGLTMSSDSFVRNKPFFDNSYINPDHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKTKLIILSTHKDPSFPKLSVSNHHQQLMAQGKANVRHGSSSFAMSMLLLSIFSWRLGT
Sbjct:   17 LISYSIVNGLTMSSDSFVRNKPFFDNSYINPDHVVLSAAVCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQEMEQISKTKLIILSTHKDPSFPKLSVSNHHQQLMAQGKANVRHGSSSFAMSMLLLSIFSWRLGT 768          
BLAST of Anosmin-1 vs. Planmine SMEST
Match: SMESG000069895.1 (SMESG000069895.1)

HSP 1 Score: 246.899 bits (629), Expect = 1.254e-69
Identity = 171/628 (27.23%), Postives = 309/628 (49.20%), Query Frame = 2
Query:  470 QEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD----RECHLDQQCMIEGDVRKCCNLRC----VVPQFH---PKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPV-KLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGP----QDGRIKISEPYYHKNSLKIDLFITNSKK-NLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRE-RGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKR-KIKKDVNLDDEQFVLTFCFCTPSCLKL----ISQPQYQSTNLRCL-----NRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            +E    CT  C+     GL    C   C  +  GCL GC+FL   + +  G CP   + + +       +C  D++C     + KCC + C    V P ++   P++   L P  I    + S  E  W     +F     P+ ++LQ R    R F+ + A  W+TL+IT + G  L +   G  YQFR+A+V+  G  GF   S P  ++ + P++P   + L++   R+ + GKI++ + W++   +  P++EY + W+ D+G+ Q DG ++ ALT F Q+VP ++ +Y +E L  + TY+V+V     W G  AL+S P+T+FL+TP L     E + ++D     G   +C +       D  + +S P Y      +D+ I ++K  + +Y++S+ P +CID            P  + ++E +R  R I +     ++  L F+C+Y +Q++ + ++  K+  + K + ++         FC PSC  +     S+P   +  L  +     N  + ++ P +K  ++ S ++P S +V  E     DI    + R+R+ WGP +++ + +          ++P +D S++  KV+ ++ +SI L NL+  T YIV+ Q +
Sbjct:   30 KESKDQCTKACK-----GLPNM-CDIGCPSSEPGCLSGCKFLKETLDLHSGKCPSTLDTNPIKQPSCLSKCKSDKECF--NTLHKCCKIGCSNICVEPIYNDSIPELPAQLIPIKI---PNSSDIEFEWMSQYKTFINEKNPLFFILQTRVCSCRKFDESYADKWQTLIITSQSGARLNEFDHGKIYQFRLAAVSADGARGFGVPSTPFPEIVERPSNPASVKGLSELLTRVYSTGKISVTIRWLKSDEYVFPISEYAVKWMIDYGYVQSDGHAISALTQFTQSVPPDKTAYVLEDLKPNTTYRVEVLTKVYWNGDEALVSEPSTLFLSTPSLTHGRLEEIFNQD---YQGPATNCKNSSMLRHNDVTVYLSNPIYKNGETSLDIKINDNKDLSSLYRVSFTPKICID------------PLDNQMSEQHRSTRTIESKNNVISIENLYFNCQYYIQIEIISLKSFKKHHLLKPIQIEGY-------FCVPSCTNIASSEASEPPKCADYLTLIPSQPENLTSKLINPTSKT-YELSWRHPISVMVRHE-----DIY---HSRYRITWGPRLNEPIKQSMY--ATYSGIVPIMDSSKSNVKVINKDANSIILTNLQPRTMYIVKAQLL 613          
BLAST of Anosmin-1 vs. Planmine SMEST
Match: SMESG000069895.1 (SMESG000069895.1)

HSP 1 Score: 246.514 bits (628), Expect = 2.218e-69
Identity = 179/664 (26.96%), Postives = 323/664 (48.64%), Query Frame = 2
Query:  362 LQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCPEKFEIDKLLD----RECHLDQQCMIEGDVRKCCNLRC----VVPQFH---PKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPV-KLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKW-GSAALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGP----QDGRIKISEPYYHKNSLKIDLFITNSKK-NLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRE-RGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKR-KIKKDVNLDDEQFVLTFCFCTPSCLKL----ISQPQYQSTNLRCL-----NRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            L +  + S+P N  Q D T A +    +  +     +E    CT  C+     GL    C   C  +  GCL GC+FL   + +  G CP   + + +       +C  D++C     + KCC + C    V P ++   P++   L P  I    + S  E  W     +F     P+ ++LQ R    R F+ + A  W+TL+IT + G  L +   G  YQFR+A+V+  G  GF   S P  ++ + P++P   + L++   R+ + GKI++ + W++   +  P++EY + W+ D+G+ Q DG ++ ALT F Q+VP ++ +Y +E L  + TY+V+V     W G  AL+S P+T+FL+TP L     E + ++D     G   +C +       D  + +S P Y      +D+ I ++K  + +Y++S+ P +CID            P  + ++E +R  R I +     ++  L F+C+Y +Q++ + ++  K+  + K + ++         FC PSC  +     S+P   +  L  +     N  + ++ P +K  ++ S ++P S +V  E     DI    + R+R+ WGP +++ + +          ++P +D S++  KV+ ++ +SI L NL+  T YIV+ Q +
Sbjct:   17 LNVFISMSRPSNAYQ-DLTFASAQCYALCLKNFLKIKESKDQCTKACK-----GLPNM-CDIGCPSSEPGCLSGCKFLKETLDLHSGKCPSTLDTNPIKQPSCLSKCKSDKECF--NTLHKCCKIGCSNICVEPIYNDSIPELPAQLIPIKI---PNSSDIEFEWMSQYKTFINEKNPLFFILQTRVCSCRKFDESYADKWQTLIITSQSGARLNEFDHGKIYQFRLAAVSADGARGFGVPSTPFPEIVERPSNPASVKGLSELLTRVYSTGKISVTIRWLKSDEYVFPISEYAVKWMIDYGYVQSDGHAISALTQFTQSVPPDKTAYVLEDLKPNTTYRVEVLTKVYWNGDEALVSEPSTLFLSTPSLTHGRLEEIFNQD---YQGPATNCKNSSMLRHNDVTVYLSNPIYKNGETSLDIKINDNKDLSSLYRVSFTPKICID------------PLDNQMSEQHRSTRTIESKNNVISIENLYFNCQYYIQIEIISLKSFKKHHLLKPIQIEGY-------FCVPSCTNIASSEASEPPKCADYLTLIPSQPENLTSKLINPTSKT-YELSWRHPISVMVRHE-----DIY---HSRYRITWGPRLNEPIKQSMY--ATYSGIVPIMDSSKSNVKVINKDANSIILTNLQPRTMYIVKAQLL 635          
BLAST of Anosmin-1 vs. Planmine SMEST
Match: SMESG000067656.1 (SMESG000067656.1)

HSP 1 Score: 172.555 bits (436), Expect = 2.729e-44
Identity = 174/688 (25.29%), Postives = 287/688 (41.72%), Query Frame = 2
Query:  332 VCKASCYHKCLQM--QANSSQPGNQMQQDSTIAHSPAS------------------VIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGN-CPEKFEID----KLLDRECHLDQQCMIEGDVR--------KCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSP--PVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGS-AALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSD--ILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLL-----LPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            +  A CY KCLQ+  Q  S+ P +Q   ++    SPA                   V +Q       +  T C+  C  S S  + G DC   CQP+  GC+  CQF     K S  + CP    +D    K L+ +C   + C  + D          + C++ C+  +   K+    + P   +     + E  W      F     P++++LQ R+     F    A+ W+TL+ T +  V L     G  YQFR+A+V   G++GF   S   P  +++ P       N+ D  W+I    K  + + W +         E+++SW+ DHG  QPDG+ L +LT + +T+PGN+    I+ L    TYKVQ+     W S   + S P+TI++ TP           +KDS+    ++       +D +I+   P      + ID+ I +      Y +   P  CI+T S +L                R R          L  L+F CRY+++V            K    + +  + L  CFCTP C    S  QYQS      + + P  + P  +L    + +  ++ + W+ + +       L   ++R+ W P       RKF    I   L      P I++ +++ K++  NV+   L NL K   Y++++Q++
Sbjct:   11 IAAARCYSKCLQIFIQFKSTFPMSQTGPEN-FTRSPAKNQGTRLASFVDETAHHLLVTKQDILSCLNDTKTGCSKQCIQSCS--MVG-DCI--CQPSDIGCMLACQFRKKINKESSPDECPAMSSLDETTMKNLNSQC--PKNCKTKSDCHPNQLCCPVESCDMICLQIE-RRKLNYPKYKPVPTKLNTSVNIEFDWSQTFKPFLNKDSPLIFILQTRSCLCHKFNDKYATPWQTLIRTHKLRVELDTFDKGTIYQFRLAAVQHNGSDGFGPISDNLPTVISRPPPPLPP-ENVIDYKWQITDENKFRVEIKWSKSVDPFWRSYEFQVSWMVDHGVAQPDGEPLSSLTQYSKTLPGNEDRCTIDSLKPATTYKVQIVVKVFWPSFGPITSIPSTIYIATPN----------TKDSTEFYLEKKHA--NKKDFKIRSIHPSNRNLQVVIDMKIFSD-----YLVEGYPEACINTGSENL-----------SKSFQRFRKTYKRTDQLILNNLRFHCRYSVEV-----------TKTSGKVPNATYHL--CFCTPPCS---SNNQYQSKYCNKEDISLPSPKEPRSVLL---NLDTMTYKISWKPSHRISPRSRFLGVTKYRISWAP-------RKFEPVNIEHYLETKDFKPLINLDESDVKIVHSNVTLFLLHNLTKEQVYVIRIQSL 634          
BLAST of Anosmin-1 vs. Planmine SMEST
Match: SMESG000067656.1 (SMESG000067656.1)

HSP 1 Score: 170.244 bits (430), Expect = 1.809e-43
Identity = 179/702 (25.50%), Postives = 292/702 (41.60%), Query Frame = 2
Query:  332 VCKASCYHKCLQM--QANSSQPGNQMQQDSTIAHSPAS------------------VIEQQCKKFNQEFTTSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGN-CPEKFEID----KLLDRECHLDQQCMIEGDVR--------KCCNLRCVVPQFHPKIGPSLWPPTIMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNWKTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSP--PVKLAKTPASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHGFTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGS-AALISTPNTIFLTTPQLP-----VRESERLV---SKDSSSLSGD---QCSCVD---GPQDGRIKISEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPSPSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKDVNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKILFQRSHKNPESFLVFWEYTEKSD--ILLLPNVRFRVIWGPSIDDHVDRKFMQPGINQLL-----LPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAI 2266
            +  A CY KCLQ+  Q  S+ P +Q   ++    SPA                   V +Q       +  T C+  C  S S  + G DC   CQP+  GC+  CQF     K S  + CP    +D    K L+ +C   + C  + D          + C++ C+  +   K+    + P   +     + E  W      F     P++++LQ R+     F    A+ W+TL+ T +  V L     G  YQFR+A+V   G++GF   S   P  +++ P       N+ D  W+I    K  + + W +         E+++SW+ DHG  QPDG+ L +LT + +T+PGN+    I+ L    TYKVQ+     W S   + S P+TI++ TP          ES +L     K  + L  +   +CSC +     +D +I+   P      + ID+ I +      Y +   P  CI+T S +L                R R          L  L+F CRY+++V            K    + +  + L  CFCTP C    S  QYQS      + + P  + P  +L      +  ++ + W+ + +       L   ++R+ W P       RKF    I   L      P I++ +++ K++  NV+   L NL K   Y++++Q++
Sbjct:   11 IAAARCYSKCLQIFIQFKSTFPMSQTGPEN-FTRSPAKNQGTRLASFVDETAHHLLVTKQDILSCLNDTKTGCSKQCIQSCS--MVG-DCI--CQPSDIGCMLACQFRKKINKESSPDECPAMSSLDETTMKNLNSQC--PKNCKTKSDCHPNQLCCPVESCDMICLQIE-RRKLNYPKYKPVPTKLNTSVNIEFDWSQTFKPFLNKDSPLIFILQTRSCLCHKFNDKYATPWQTLIRTHKLRVELDTFDKGTIYQFRLAAVQHNGSDGFGPISDNLPTVISRPPPPLPP-ENVIDYKWQITDENKFRVEIKWSKSVDPFWRSYEFQVSWMVDHGVAQPDGEPLSSLTQYSKTLPGNEDRCTIDSLKPATTYKVQIVVKVFWPSFGPITSIPSTIYIATPNTKDSTEFYLESTKLFIFGIKIYNHLFQNKIKECSCPEKHANKKDFKIRSIHPSNRNLQVVIDMKIFSD-----YLVEGYPEACINTGSENL-----------SKSFQRFRKTYKRTDQLILNNLRFHCRYSVEV-----------TKTSGKVPNATYHL--CFCTPPCS---SNNQYQSKYCNKEDISLPSPKEPRSVLLNL---DTMTYKISWKPSHRISPRSRFLGVTKYRISWAP-------RKFEPVNIEHYLETKDFKPLINLDESDVKIVHSNVTLFLLHNLTKEQVYVIRIQSL 660          
The following BLAST results are available for this feature:
BLAST of Anosmin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ANOS14.833e-3724.59anosmin 1 [Source:HGNC Symbol;Acc:HGNC:6211][more]
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BLAST of Anosmin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
kal-11.047e-2024.12KALlmann syndrome homolog [Source:UniProtKB/TrEMB... [more]
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BLAST of Anosmin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Kal18.910e-1222.12gene:FBgn0039155 transcript:FBtr0084549[more]
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BLAST of Anosmin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
anos1a2.060e-2925.80anosmin 1a [Source:ZFIN;Acc:ZDB-GENE-000201-9][more]
anos1b2.962e-2922.83anosmin 1b [Source:ZFIN;Acc:ZDB-GENE-000201-13][more]
anos1b2.962e-2922.83anosmin 1b [Source:ZFIN;Acc:ZDB-GENE-000201-13][more]
ANOS17.617e-2628.44anosmin-1-like [Source:NCBI gene;Acc:100535716][more]
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BLAST of Anosmin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
psmc42.181e-3924.92proteasome 26S subunit, ATPase 4 [Source:Xenbase;A... [more]
psmc42.333e-3924.30proteasome 26S subunit, ATPase 4 [Source:Xenbase;A... [more]
psmc43.957e-3923.56proteasome 26S subunit, ATPase 4 [Source:Xenbase;A... [more]
psmc45.188e-3924.30proteasome 26S subunit, ATPase 4 [Source:Xenbase;A... [more]
psmc46.781e-3925.21proteasome 26S subunit, ATPase 4 [Source:Xenbase;A... [more]
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BLAST of Anosmin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Anosmin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P23352|KALM_HUMAN2.321e-3624.59Anosmin-1 OS=Homo sapiens OX=9606 GN=ANOS1 PE=1 SV... [more]
sp|P33005|KALM_CHICK5.265e-3423.73Anosmin-1 OS=Gallus gallus OX=9031 GN=ANOS1 PE=2 S... [more]
sp|Q90369|KALM_COTJA6.686e-3323.76Anosmin-1 OS=Coturnix japonica OX=93934 GN=ANOS1 P... [more]
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BLAST of Anosmin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RJ346.127e-6727.69Anosmin-1 OS=Fasciola hepatica OX=6192 GN=D915_003... [more]
A0A504YS356.943e-6627.53Anosmin-1 OS=Fasciola gigantica OX=46835 GN=FGIG_0... [more]
A0A095C8H71.686e-6429.37Anosmin-1 (Fragment) OS=Schistosoma haematobium OX... [more]
A0A094ZMF22.401e-6328.10Anosmin-1 OS=Schistosoma haematobium OX=6185 GN=MS... [more]
A0A430QBF15.113e-6329.20Uncharacterized protein OS=Schistosoma bovis OX=61... [more]
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BLAST of Anosmin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
anos1a1.083e-3025.74anosmin-1-like [Source:NCBI gene;Acc:103026549][more]
anos1b1.742e-2825.15anosmin-1 [Source:NCBI gene;Acc:103026226][more]
ANOS13.000e-2624.36anosmin 1 [Source:HGNC Symbol;Acc:HGNC:6211][more]
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BLAST of Anosmin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Anosmin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Anosmin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO366681.491e-1124.01Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Anosmin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
anos13.124e-3824.90anosmin 1 [Source:NCBI gene;Acc:100049494][more]
anos18.951e-3824.78anosmin 1 [Source:NCBI gene;Acc:100049494][more]
anos1b2.172e-1922.77anosmin 1b [Source:NCBI gene;Acc:100049297][more]
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BLAST of Anosmin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000010608.10.000e+099.87SMESG000010608.1[more]
SMESG000069895.11.254e-6927.23SMESG000069895.1[more]
SMESG000069895.12.218e-6926.96SMESG000069895.1[more]
SMESG000067656.12.729e-4425.29SMESG000067656.1[more]
SMESG000067656.11.809e-4325.50SMESG000067656.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004938 ID=SMED30004938|Name=Anosmin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=3264bp
GTGTTCCACTTGATTGCACAGCAACAACCTCACTGCATTCAATGAGTCAA
GTCTCTTCTGATTGGCTAATCGATCAGACCAATCAAATTCATATCAATTC
TACCTTCATTATTCAAATTCTAAATAGACGTTCAATTTTATTATTATTTT
TATTTTTGTCAATTGAGATGAGTCGGAATTTATGCAGCGTGGATTTAATC
GTCATCCTTATCGTGTGATTTCCTATTCGATCGTCAACGGTTTAACGATG
TCGTCGGATTCGTTTGTCAGAAATAAACCGTTTTTCGACAACTCATACAT
CAATCCCGATCATGTGGTATTGAGTGCAGCGGTTTGCAAAGCAAGTTGCT
ATCATAAATGTCTGCAGATGCAAGCCAATTCCAGTCAGCCCGGCAATCAG
ATGCAGCAAGACTCCACAATTGCTCACAGTCCTGCGAGTGTCATAGAACA
ACAGTGCAAGAAGTTCAACCAGGAATTCACAACATCGTGCACAACGGGTT
GTGAATATTCCGGGAGCAGTGGACTCGGTGGCAAGGACTGCAGCAACCAA
TGTCAACCAAACAGTAGTGGATGTCTTGAAGGCTGTCAATTTCTCAACAC
TGCCATAAAAATGTCTCTAGGGAATTGCCCTGAAAAATTCGAAATAGATA
AACTTCTTGATAGAGAATGCCACCTTGATCAGCAGTGCATGATAGAAGGA
GACGTAAGGAAGTGCTGCAACCTCCGATGTGTTGTTCCTCAGTTTCATCC
AAAAATTGGACCAAGTTTATGGCCTCCCACAATAATGGAAGGAAAAGATG
GCTCGTCTTTTGAAATGACATGGAAATACGATGACACATCTTTCTCCGAG
CCAATGCTTCCAATTTTGTATGTTTTACAAGTAAGAACGTATTTCAATCG
AGATTTCGAAACCACCAAGGCGTCCAATTGGAAAACCTTATTAATAACAT
TCAGAGAAGGAGTAAATTTGCAGGACCTGCAGCCTGGAATGTGGTATCAA
TTCAGAGTAGCATCTGTAACAAAGTACGGAACAAATGGTTTCAAATCATT
TTCACCGCCAGTAAAATTAGCAAAAACTCCTGCTAGCCCCGGAGTACCTA
GAAACCTTACCGATGGGGCTTGGCGTATTAGAGCCAATGGGAAAATAAAT
ATTCGAGTTCACTGGATGGAGCCAGCTGCCTTTGACATTCCTGTTACAGA
ATATAAGATTTCATGGGTCCGTGATCATGGATTTACTCAGCCAGATGGGC
AATCTTTGGAGGCCCTCACAGATTTTGTTCAAACAGTTCCTGGGAATCAA
AGAAGCTATGACATAGAAGGATTGGTGGCAGATAAAACCTACAAAGTTCA
GGTGCGCGCCATTTGCAAATGGGGCTCAGCTGCTCTGATATCGACACCGA
ACACAATTTTCCTGACTACTCCTCAACTGCCAGTTCGAGAATCTGAACGC
CTCGTCAGTAAAGATTCCTCATCATTGTCAGGAGATCAGTGCAGTTGTGT
TGATGGGCCACAAGATGGAAGAATCAAGATCAGTGAACCTTATTATCATA
AAAACTCCCTCAAAATCGATTTATTTATCACAAACTCGAAAAAGAATTTG
GTCTATAAGATTTCCTATGCTCCAAATGTTTGCATTGATACAGAATCCTC
ATCATTATTCGTCAATGACCTTCAACCTTCACCGAGTGATATTAACGAAA
TTTACCGAGAAAGAGGTATCACTGCGGCGTTACCAAACGCTACCCTTGGG
CCGTTGCAATTCAGCTGTAGATACACTCTACAAGTACAACCTTTGAGAAT
TCGCCCAAGTAAAAGAAAGATTAAAAAGGACGTTAACTTAGATGATGAAC
AATTCGTGTTGACATTTTGTTTTTGTACACCTTCGTGCTTGAAACTGATC
TCCCAACCCCAGTACCAATCGACAAATCTCAGATGTCTGAATAGAGCCAA
CCCAATATTAGAACCGCCAAATAAAATCCTCTTTCAAAGATCTCATAAGA
ATCCTGAATCGTTTCTCGTATTTTGGGAGTATACAGAGAAGTCAGATATC
CTTTTATTGCCGAATGTCAGATTCCGTGTGATTTGGGGCCCGTCAATAGA
TGATCATGTTGACCGAAAGTTTATGCAGCCCGGCATCAATCAGTTACTGC
TGCCTAGGATAGACATGTCACAAGCGGAAACTAAAGTACTGAAAGAAAAC
GTGTCATCAATTTGGCTGGAGAATTTGAAAAAGAACACATTCTATATTGT
GCAAGTCCAAGCTATCTTCTATCAACAAGAGATGGAGCAGATCAGTAAAA
CAAAATTAATTATTTTATCAACTCATAAAGATCCATCATTCCCGAAGTTG
TCAGTTTCCAATCACCACCAGCAGCTAATGGCACAGGGCAAGGCAAATGT
CCGTCACGGTTCATCATCATTTGCTATGTCAATGTTGCTACTGTCCATCT
TCAGTTGGCGCCTCGGAACGTGAATTGGTAACTGGCGGGTGATGCTACCA
GAGCGCCGCCACCGCCGCCACCGCTCAACCACATTGCTGCTGTGATAGTA
GTTCCTCTATCGTCGTTGTAGTCGTTGTTGTTGTTTTCGTCTCATTGATG
ATCCCGCATTCCGGTCAAAACCATGAAAATTGCATCTACAACCTGTAAAG
AACTTGACAACCTTAATTCGTATCTACAGCGTTCTGTGTTGCGAACTTCG
TGTGATTATTATATATAGTTATCCTTTTCTGCCTCCATTTCACCTGTGTA
TTAACTTATTAATCATTGACAGACAATTAATAAAGCCCAATTGATAGCAT
CGAGGACCCCCCGTTCACATGGAGGCCACAAACGATTGAAAGATTTTTAT
AAAATATTATAAACTTATCATTCATGCAAATGATTTCTCTGTTAAAATTT
AATGCCAAATTGCGGTGAGCATTTCCGAGTTTATGGGAGAAATTCTAGGA
AATGTGCCGCAGAGCGAGAAGTCTGGGAATTTCCGAGAAATGTCTCTGAA
AATATAATGACGAAATGCACCTCTAATCTGGAGTGTAATGAAATCAATTT
AAGGGAAGTCATTCACATTTCATTTCACATCTCTCCAATCCCAGCTATCA
AGTCAAATGCGCGGGTTGGCTAATTGTCAAATCGGATTGAAGCATCCAGG
CGCGCTTCATTCCCATTGGACAATCGGAAATCAATATAATAATTATTAAT
CGATCCGATAAATTCAATTATTTGATTGGTTGATAACTTTGGAAGATGAA
TTCAAATTTTAATT
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protein sequence of SMED30004938-orf-1

>SMED30004938-orf-1 ID=SMED30004938-orf-1|Name=SMED30004938-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=764bp
MQRGFNRHPYRVISYSIVNGLTMSSDSFVRNKPFFDNSYINPDHVVLSAA
VCKASCYHKCLQMQANSSQPGNQMQQDSTIAHSPASVIEQQCKKFNQEFT
TSCTTGCEYSGSSGLGGKDCSNQCQPNSSGCLEGCQFLNTAIKMSLGNCP
EKFEIDKLLDRECHLDQQCMIEGDVRKCCNLRCVVPQFHPKIGPSLWPPT
IMEGKDGSSFEMTWKYDDTSFSEPMLPILYVLQVRTYFNRDFETTKASNW
KTLLITFREGVNLQDLQPGMWYQFRVASVTKYGTNGFKSFSPPVKLAKTP
ASPGVPRNLTDGAWRIRANGKINIRVHWMEPAAFDIPVTEYKISWVRDHG
FTQPDGQSLEALTDFVQTVPGNQRSYDIEGLVADKTYKVQVRAICKWGSA
ALISTPNTIFLTTPQLPVRESERLVSKDSSSLSGDQCSCVDGPQDGRIKI
SEPYYHKNSLKIDLFITNSKKNLVYKISYAPNVCIDTESSSLFVNDLQPS
PSDINEIYRERGITAALPNATLGPLQFSCRYTLQVQPLRIRPSKRKIKKD
VNLDDEQFVLTFCFCTPSCLKLISQPQYQSTNLRCLNRANPILEPPNKIL
FQRSHKNPESFLVFWEYTEKSDILLLPNVRFRVIWGPSIDDHVDRKFMQP
GINQLLLPRIDMSQAETKVLKENVSSIWLENLKKNTFYIVQVQAIFYQQE
MEQISKTKLIILSTHKDPSFPKLSVSNHHQQLMAQGKANVRHGSSSFAMS
MLLLSIFSWRLGT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005576extracellular region
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR003961FN3_dom
IPR013783Ig-like_fold
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding
GO:0030414peptidase inhibitor activity
Vocabulary: biological process
TermDefinition
GO:0010466negative regulation of peptidase activity