phosphatidylinositol 4-kinase beta

Overview
Namephosphatidylinositol 4-kinase beta
Smed IDSMED30004928
Length (bp)2653
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of phosphatidylinositol 4-kinase beta (SMED30004928) t-SNE clustered cells

Violin plots show distribution of expression levels for phosphatidylinositol 4-kinase beta (SMED30004928) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of phosphatidylinositol 4-kinase beta (SMED30004928) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for phosphatidylinositol 4-kinase beta (SMED30004928) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004928

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30004928h1SMcG0017164 tr5_4416mu_Smed_v1PMID:25558068
Reuter et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
nervous systemSMED30004928h1SMcG0017164 dd_Smed_v4_6071_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Match: PI4KB (phosphatidylinositol 4-kinase beta [Source:HGNC Symbol;Acc:HGNC:8984])

HSP 1 Score: 484.567 bits (1246), Expect = 3.436e-158
Identity = 271/569 (47.63%), Postives = 348/569 (61.16%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDL---VLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    + S       LSS+L     +P +E +++       L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASIS-------LSSNLKRTASNPKVENEDE----PVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 800          

HSP 2 Score: 85.1149 bits (209), Expect = 3.515e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Match: PI4KB (phosphatidylinositol 4-kinase beta [Source:HGNC Symbol;Acc:HGNC:8984])

HSP 1 Score: 484.567 bits (1246), Expect = 3.436e-158
Identity = 271/569 (47.63%), Postives = 348/569 (61.16%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDL---VLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    + S       LSS+L     +P +E +++       L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASIS-------LSSNLKRTASNPKVENEDE----PVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 800          

HSP 2 Score: 85.1149 bits (209), Expect = 3.515e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Match: PI4KB (phosphatidylinositol 4-kinase beta [Source:HGNC Symbol;Acc:HGNC:8984])

HSP 1 Score: 483.026 bits (1242), Expect = 2.333e-157
Identity = 270/573 (47.12%), Postives = 345/573 (60.21%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  263 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 827          

HSP 2 Score: 85.1149 bits (209), Expect = 3.545e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  133 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 231          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Match: PI4KB (phosphatidylinositol 4-kinase beta [Source:HGNC Symbol;Acc:HGNC:8984])

HSP 1 Score: 482.641 bits (1241), Expect = 2.409e-157
Identity = 270/573 (47.12%), Postives = 345/573 (60.21%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815          

HSP 2 Score: 85.1149 bits (209), Expect = 3.536e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Match: PI4KB (phosphatidylinositol 4-kinase beta [Source:HGNC Symbol;Acc:HGNC:8984])

HSP 1 Score: 469.159 bits (1206), Expect = 1.218e-156
Identity = 252/491 (51.32%), Postives = 317/491 (64.56%), Query Frame = 1
Query: 1216 VSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    E   + +          N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:    1 MAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 483          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Match: pifk-1 (PhosphoInositide Four Kinase (PI-4 Kinase) [Source:UniProtKB/TrEMBL;Acc:Q20077])

HSP 1 Score: 364.77 bits (935), Expect = 2.004e-114
Identity = 212/489 (43.35%), Postives = 291/489 (59.51%), Query Frame = 1
Query: 1153 KNKCS-CASYFLDPQLSFIKILVSISMRLTRY---PSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSG----------KSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGIQ 2577
            K++C   ++  L  Q +FI  LV I ++LT +    SK +KT  L++EL+ LN  LP  VWIP +   + +  I  N +  LNSK + PY+ + EV+ ++  +                                  NIK +   +                I++   E++ L+   ++   + DPS +V  E W +K  RI+ SS Y   + W+L+ VIVK GDDL QE  A Q+L   + +W  E  PL++RP  IV I SD+G+IEPVL+ +S+HQIKR  +            SL  +F+N FGP NSE+++NAQK F++S AAYSLV Y LQ+KDRHNGNILLD +GH+IHIDYGF+LS+SP RNLGFE +PFKLT E +D+MGG+ SDMF YFK L+L+GL+AARKH  +I++  EI+ + S K+ CF  G ETV      RFH+  T+ QLQ LVD++VE S  S TTR YDSFQYYTNGI 
Sbjct:  230 KSRCGPISTKLLTNQQNFIGRLVYIGVKLTEHHLLSSKQEKTIALQSELSMLNSMLPNSVWIP-FDFENTMFNICVNESAVLNSKDKVPYVFFAEVIRSSPNY--------------------------------NSNIKTLSSVEPR--------------IKKSQSESVLLK--NKSYHDSTDPSIAVFAESWAEKKARIRGSSRYENHQKWDLIPVIVKTGDDLSQEAFAQQLLFTFKDLWHEEGVPLYLRPYKIVCIGSDAGLIEPVLDTLSLHQIKRHLTNVFRMNGNVTTPSLKHHFENVFGPVNSETYVNAQKNFIQSTAAYSLVSYYLQLKDRHNGNILLDMDGHLIHIDYGFLLSSSP-RNLGFETAPFKLTTEIIDVMGGIDSDMFLYFKSLLLRGLMAARKHHRRIVSLAEIMSAGS-KMQCFRAGAETV-RALEARFHVSSTDEQLQQLVDTLVEGSRDSYTTRFYDSFQYYTNGIH 666          

HSP 2 Score: 60.8474 bits (146), Expect = 3.404e-9
Identity = 28/101 (27.72%), Postives = 54/101 (53.47%), Query Frame = 1
Query:  160 CTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYV 462
             T  T ++Q   +RL  S  F+  +A++YL+   +     FL  + F  P+  +DF+LPQL+ L+V     + V  +Y   R K+  ++++  +WL++ + 
Sbjct:   51 TTKPTHDRQSWTMRLISSESFNAPMAVEYLNSIQDKPSLTFLGKRLFDLPEKDLDFYLPQLMYLYVTKVDAASVSHAYIEERNKKDAQFTVLCIWLLNCFT 151          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Match: Y75B8A.24 (pep chromosome:WBcel235:III:12251494:12273692:-1 gene:WBGene00013557.1 transcript:Y75B8A.24.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y75B8A.24)

HSP 1 Score: 185.267 bits (469), Expect = 1.188e-47
Identity = 104/262 (39.69%), Postives = 156/262 (59.54%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++++ VW     P+ V P  +V  S   G+IE V N+ S  Q+ R  +   L EYFK Q+G  +SE+F  A++ FV S AAYS+  +LLQ+KDRHNGNI++D +GH+IHID+GF+  +SPG NLGFE   FKL+ E V IMGG +++  F  F  L +   LA R +    ++ + ++  +   LPCF +G     N  R RF     E      + S++ N   ++ +++YD  QY+ N I
Sbjct: 1850 AAIFKVGDDVRQDMLALQLMQLMKNVWAGLGLPVRVFPYRVVATSPGCGVIECVPNSKSRDQLGR-QTDFGLYEYFKTQYGDESSETFQEARRNFVRSMAAYSVFSFLLQIKDRHNGNIMIDLDGHIIHIDFGFMFESSPGGNLGFE-PDFKLSEEMVAIMGGKMEAAPFRQFASLCVHAYLAVRPYHKSFVSLVSLM--LDTHLPCF-RG--KTINQLRARFAPEMNEKDAAKYMYSVITNCFLNIRSKMYDQLQYFQNEI 2104          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Match: Y75B8A.24 (pep chromosome:WBcel235:III:12251492:12273692:-1 gene:WBGene00013557.1 transcript:Y75B8A.24.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y75B8A.24)

HSP 1 Score: 185.267 bits (469), Expect = 1.188e-47
Identity = 104/262 (39.69%), Postives = 156/262 (59.54%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++++ VW     P+ V P  +V  S   G+IE V N+ S  Q+ R  +   L EYFK Q+G  +SE+F  A++ FV S AAYS+  +LLQ+KDRHNGNI++D +GH+IHID+GF+  +SPG NLGFE   FKL+ E V IMGG +++  F  F  L +   LA R +    ++ + ++  +   LPCF +G     N  R RF     E      + S++ N   ++ +++YD  QY+ N I
Sbjct: 1850 AAIFKVGDDVRQDMLALQLMQLMKNVWAGLGLPVRVFPYRVVATSPGCGVIECVPNSKSRDQLGR-QTDFGLYEYFKTQYGDESSETFQEARRNFVRSMAAYSVFSFLLQIKDRHNGNIMIDLDGHIIHIDFGFMFESSPGGNLGFE-PDFKLSEEMVAIMGGKMEAAPFRQFASLCVHAYLAVRPYHKSFVSLVSLM--LDTHLPCF-RG--KTINQLRARFAPEMNEKDAAKYMYSVITNCFLNIRSKMYDQLQYFQNEI 2104          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Match: piki-1 (Phosphatidylinositol 3-kinase piki-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EDY0])

HSP 1 Score: 86.2705 bits (212), Expect = 7.788e-17
Identity = 72/267 (26.97%), Postives = 123/267 (46.07%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLI--EYFKNQFGPTNSESFL--NAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYG-FILSASPGRNLGFEFSPFKLTYEEVDIM--GGVKSDMFEYFKILILQGLLAARKHMD------KILATIEI----LDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRL 2541
            S+I K  DD+RQ+    ++L  + ++W S    L +    I+ +    GM E VLN  ++ +I++    + ++  E  +      NS+ F    AQ+ F+ SCA + +V Y+L + DRHN NIL    GH+ HID+G ++           +  PF  T E   ++  G   +   + F     +     R++ +      +I+A  +I    +DS++F           V NN      L  T+A +Q    +M++NSL S   RL
Sbjct: 1055 SIIHKRDDDMRQDAFVMKMLNEMDRIWKSNGLDLRMITFRIMPVGYRRGMGELVLNCATLMEIQKEEGLRGVLNDEILRKWLMKHNSDEFAYKEAQENFIRSCAGWCIVTYVLGIGDRHNDNILFTKNGHVFHIDFGKYMGDWQMAAGFRRDRVPFVFTTEMFHVINNGRAPTQYNQKFIDYCCKAFNHLRRNKNTLTNLLRIMACSDIPGINMDSLAF-----------VENNL--MLDLSDTDATVQFT--AMIQNSLGSAFVRL 1306          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Match: vps-34 (Phosphatidylinositol 3-kinase catalytic subunit type 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXI7])

HSP 1 Score: 84.7297 bits (208), Expect = 1.212e-16
Identity = 68/267 (25.47%), Postives = 121/267 (45.32%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSL------IEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            +VI K GDDLRQ+QL  Q+++++  +    +  L + P  ++      G ++  + +  +  I+  Y    +      ++  +   GP   E   N    +V S A YS++ Y+L + DRH  N+LL   G + H+D+GFIL    GR+      P KLT E V +MGGVKS  F  F   +       R+H + +L    ++  +   +P  +   +        R  L  ++      + + +E+SL++    + D    Y   +
Sbjct:  392 TVIFKTGDDLRQDQLIQQMVRLIDIILKKGQLDLKLTPYLVLSTGVGQGFVQ-CIKSKPLRAIQEQYKAHKMDCIREAMKELRPGDGPFGIEP--NVIDNYVRSLAGYSVIMYILGLGDRHLDNLLLCENGKLFHVDFGFIL----GRDPKPMPPPMKLTSEMVQVMGGVKSKQFLEFVQHVDSAYRILRRHSNVLLNLFSLM--LDAGIPDIAAEPDKAIFKIEQRLRLDLSDEAATKHIFTQIESSLNAKMAMISDIIHAYKQNL 649          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Match: fwd (gene:FBgn0004373 transcript:FBtr0072517)

HSP 1 Score: 352.829 bits (904), Expect = 2.383e-104
Identity = 172/318 (54.09%), Postives = 230/318 (72.33%), Query Frame = 1
Query: 1621 GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            GD+R R   N+  +  +      +DPS++ L EPW +K + I+ESSPYG + NW LLS IVK GDDLRQE +A Q+L+M + +W  E+  LWVRP  IV +S+DSG+IEP+LN VS+HQIK+  S KSL EYF +++G  + ESF  AQK FV+SCAAY L+ YLLQVKDRHNGNIL  ++GH+IHID+GFILS SP +NLGFE SPFKLT E V++MGG  S+ +  F  L+L G+++ARKHMD+I+  +EI+ S +  LPCF  G      N R RFH+  TE +++  V+ +V++SL SL+T+LYD +QYYTNGI
Sbjct: 1023 GDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKK-NSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRS-NAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1337          

HSP 2 Score: 154.066 bits (388), Expect = 7.920e-38
Identity = 120/437 (27.46%), Postives = 187/437 (42.79%), Query Frame = 1
Query:  184 QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAY---VNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSD----LSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK 1470
            Q GLLR F+S +F++  A+ YL    E G+ +F+ NK FS+P   VD ++PQLV +++    L++V   Y T RC++S+++S+  +WL++AY   V+     +N  ++S L+L     +  EH K+ +N + +  +  +H + E R  +  +        K H RS SD    L+     ++++ S +M +      T++        C G                                       DL SGHAFDN C  F       + G    L   R + +   P                                        K S    F+   ++  K L S       +PSK +KT+ LR  L  +N NLPARVW+P+Y    H+VVRI       LNSK + PY+IYVEVV   +++   LI K
Sbjct:  563 QSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREH-KQTQNDLKSAGTGGLH-VGEPRSVVALAK-------KTHHRSQSDATVLLADFRSPHTLSISHRM-YQQPPYTTQTLPTTPAKLCLG---------------------------------------DLTSGHAFDNGCTCFE-----TVRGQVNGLLGQRTLCSCGAP----------------------------------------KTSPQKEFMKALMNVGKNLTS-------WPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPK 898          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Match: fwd (gene:FBgn0004373 transcript:FBtr0072516)

HSP 1 Score: 354.369 bits (908), Expect = 7.770e-104
Identity = 172/318 (54.09%), Postives = 230/318 (72.33%), Query Frame = 1
Query: 1621 GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            GD+R R   N+  +  +      +DPS++ L EPW +K + I+ESSPYG + NW LLS IVK GDDLRQE +A Q+L+M + +W  E+  LWVRP  IV +S+DSG+IEP+LN VS+HQIK+  S KSL EYF +++G  + ESF  AQK FV+SCAAY L+ YLLQVKDRHNGNIL  ++GH+IHID+GFILS SP +NLGFE SPFKLT E V++MGG  S+ +  F  L+L G+++ARKHMD+I+  +EI+ S +  LPCF  G      N R RFH+  TE +++  V+ +V++SL SL+T+LYD +QYYTNGI
Sbjct: 1359 GDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKK-NSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRS-NAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673          

HSP 2 Score: 154.451 bits (389), Expect = 7.775e-38
Identity = 120/437 (27.46%), Postives = 187/437 (42.79%), Query Frame = 1
Query:  184 QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAY---VNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSD----LSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK 1470
            Q GLLR F+S +F++  A+ YL    E G+ +F+ NK FS+P   VD ++PQLV +++    L++V   Y T RC++S+++S+  +WL++AY   V+     +N  ++S L+L     +  EH K+ +N + +  +  +H + E R  +  +        K H RS SD    L+     ++++ S +M +      T++        C G                                       DL SGHAFDN C  F       + G    L   R + +   P                                        K S    F+   ++  K L S       +PSK +KT+ LR  L  +N NLPARVW+P+Y    H+VVRI       LNSK + PY+IYVEVV   +++   LI K
Sbjct:  899 QSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREH-KQTQNDLKSAGTGGLH-VGEPRSVVALAK-------KTHHRSQSDATVLLADFRSPHTLSISHRM-YQQPPYTTQTLPTTPAKLCLG---------------------------------------DLTSGHAFDNGCTCFE-----TVRGQVNGLLGQRTLCSCGAP----------------------------------------KTSPQKEFMKALMNVGKNLTS-------WPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPK 1234          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Match: fwd (gene:FBgn0004373 transcript:FBtr0072515)

HSP 1 Score: 354.369 bits (908), Expect = 7.770e-104
Identity = 172/318 (54.09%), Postives = 230/318 (72.33%), Query Frame = 1
Query: 1621 GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            GD+R R   N+  +  +      +DPS++ L EPW +K + I+ESSPYG + NW LLS IVK GDDLRQE +A Q+L+M + +W  E+  LWVRP  IV +S+DSG+IEP+LN VS+HQIK+  S KSL EYF +++G  + ESF  AQK FV+SCAAY L+ YLLQVKDRHNGNIL  ++GH+IHID+GFILS SP +NLGFE SPFKLT E V++MGG  S+ +  F  L+L G+++ARKHMD+I+  +EI+ S +  LPCF  G      N R RFH+  TE +++  V+ +V++SL SL+T+LYD +QYYTNGI
Sbjct: 1359 GDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKK-NSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRS-NAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673          

HSP 2 Score: 154.451 bits (389), Expect = 7.775e-38
Identity = 120/437 (27.46%), Postives = 187/437 (42.79%), Query Frame = 1
Query:  184 QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAY---VNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSD----LSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK 1470
            Q GLLR F+S +F++  A+ YL    E G+ +F+ NK FS+P   VD ++PQLV +++    L++V   Y T RC++S+++S+  +WL++AY   V+     +N  ++S L+L     +  EH K+ +N + +  +  +H + E R  +  +        K H RS SD    L+     ++++ S +M +      T++        C G                                       DL SGHAFDN C  F       + G    L   R + +   P                                        K S    F+   ++  K L S       +PSK +KT+ LR  L  +N NLPARVW+P+Y    H+VVRI       LNSK + PY+IYVEVV   +++   LI K
Sbjct:  899 QSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREH-KQTQNDLKSAGTGGLH-VGEPRSVVALAK-------KTHHRSQSDATVLLADFRSPHTLSISHRM-YQQPPYTTQTLPTTPAKLCLG---------------------------------------DLTSGHAFDNGCTCFE-----TVRGQVNGLLGQRTLCSCGAP----------------------------------------KTSPQKEFMKALMNVGKNLTS-------WPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPK 1234          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Match: fwd (gene:FBgn0004373 transcript:FBtr0332841)

HSP 1 Score: 354.369 bits (908), Expect = 7.770e-104
Identity = 172/318 (54.09%), Postives = 230/318 (72.33%), Query Frame = 1
Query: 1621 GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            GD+R R   N+  +  +      +DPS++ L EPW +K + I+ESSPYG + NW LLS IVK GDDLRQE +A Q+L+M + +W  E+  LWVRP  IV +S+DSG+IEP+LN VS+HQIK+  S KSL EYF +++G  + ESF  AQK FV+SCAAY L+ YLLQVKDRHNGNIL  ++GH+IHID+GFILS SP +NLGFE SPFKLT E V++MGG  S+ +  F  L+L G+++ARKHMD+I+  +EI+ S +  LPCF  G      N R RFH+  TE +++  V+ +V++SL SL+T+LYD +QYYTNGI
Sbjct: 1359 GDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKK-NSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRS-NAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673          

HSP 2 Score: 154.451 bits (389), Expect = 7.775e-38
Identity = 120/437 (27.46%), Postives = 187/437 (42.79%), Query Frame = 1
Query:  184 QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAY---VNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSD----LSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK 1470
            Q GLLR F+S +F++  A+ YL    E G+ +F+ NK FS+P   VD ++PQLV +++    L++V   Y T RC++S+++S+  +WL++AY   V+     +N  ++S L+L     +  EH K+ +N + +  +  +H + E R  +  +        K H RS SD    L+     ++++ S +M +      T++        C G                                       DL SGHAFDN C  F       + G    L   R + +   P                                        K S    F+   ++  K L S       +PSK +KT+ LR  L  +N NLPARVW+P+Y    H+VVRI       LNSK + PY+IYVEVV   +++   LI K
Sbjct:  899 QSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREH-KQTQNDLKSAGTGGLH-VGEPRSVVALAK-------KTHHRSQSDATVLLADFRSPHTLSISHRM-YQQPPYTTQTLPTTPAKLCLG---------------------------------------DLTSGHAFDNGCTCFE-----TVRGQVNGLLGQRTLCSCGAP----------------------------------------KTSPQKEFMKALMNVGKNLTS-------WPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPK 1234          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Match: fwd (gene:FBgn0004373 transcript:FBtr0474147)

HSP 1 Score: 354.369 bits (908), Expect = 9.104e-104
Identity = 172/318 (54.09%), Postives = 230/318 (72.33%), Query Frame = 1
Query: 1621 GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            GD+R R   N+  +  +      +DPS++ L EPW +K + I+ESSPYG + NW LLS IVK GDDLRQE +A Q+L+M + +W  E+  LWVRP  IV +S+DSG+IEP+LN VS+HQIK+  S KSL EYF +++G  + ESF  AQK FV+SCAAY L+ YLLQVKDRHNGNIL  ++GH+IHID+GFILS SP +NLGFE SPFKLT E V++MGG  S+ +  F  L+L G+++ARKHMD+I+  +EI+ S +  LPCF  G      N R RFH+  TE +++  V+ +V++SL SL+T+LYD +QYYTNGI
Sbjct: 1359 GDVRLRHCSNLSCENTKSFSNDPEDPSAAALKEPWHEKEKLIRESSPYGHLSNWRLLSAIVKCGDDLRQELMATQLLQMFKIIWQEEQVDLWVRPYKIVCLSNDSGLIEPILNTVSLHQIKK-NSNKSLKEYFIDEYGSPSGESFRRAQKNFVQSCAAYCLISYLLQVKDRHNGNILFHSDGHIIHIDFGFILSISP-KNLGFEQSPFKLTPEFVEVMGGTSSEHWREFNKLLLVGMMSARKHMDRIINFVEIMRS-NAHLPCFKNGCSGTVQNLRKRFHMNLTEQEMERKVEQLVQDSLKSLSTKLYDGYQYYTNGI 1673          

HSP 2 Score: 154.451 bits (389), Expect = 8.357e-38
Identity = 120/437 (27.46%), Postives = 187/437 (42.79%), Query Frame = 1
Query:  184 QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAY---VNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSD----LSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK 1470
            Q GLLR F+S +F++  A+ YL    E G+ +F+ NK FS+P   VD ++PQLV +++    L++V   Y T RC++S+++S+  +WL++AY   V+     +N  ++S L+L     +  EH K+ +N + +  +  +H + E R  +  +        K H RS SD    L+     ++++ S +M +      T++        C G                                       DL SGHAFDN C  F       + G    L   R + +   P                                        K S    F+   ++  K L S       +PSK +KT+ LR  L  +N NLPARVW+P+Y    H+VVRI       LNSK + PY+IYVEVV   +++   LI K
Sbjct:  899 QSGLLRFFESPVFNIHFAVHYLFYSKEPGVLSFIGNKIFSFPDQEVDLYIPQLVVMYIQMDELAEVLDPYLTIRCRKSVDFSLKCLWLLEAYNYQVDSLGNSHNSSRKSKLALMKEIFSKREH-KQTQNDLKSAGTGGLH-VGEPRSVVALAK-------KTHHRSQSDATVLLADFRSPHTLSISHRM-YQQPPYTTQTLPTTPAKLCLG---------------------------------------DLTSGHAFDNGCTCFE-----TVRGQVNGLLGQRTLCSCGAP----------------------------------------KTSPQKEFMKALMNVGKNLTS-------WPSKAEKTSALRMSLNLINKNLPARVWLPLYSDIPHHVVRITEEKTAVLNSKDKTPYIIYVEVVEIPDIYTSPLIPK 1234          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 478.789 bits (1231), Expect = 9.362e-156
Identity = 257/502 (51.20%), Postives = 322/502 (64.14%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S   L++YF+ + G  N+E FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  341 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETQIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFRKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLKQLQIIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLLLLDYFRQEHGNFNTEEFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 834          

HSP 2 Score: 96.2857 bits (238), Expect = 7.470e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query:  133 EPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            EP+ +      NN+  KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+   WL+ AY
Sbjct:  131 EPIKSVRRRQKNNSS-KQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVYRCRQSINFSLQCAWLLGAY 239          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 478.789 bits (1231), Expect = 9.362e-156
Identity = 257/502 (51.20%), Postives = 322/502 (64.14%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S   L++YF+ + G  N+E FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  341 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETQIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFRKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLKQLQIIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLLLLDYFRQEHGNFNTEEFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 834          

HSP 2 Score: 96.2857 bits (238), Expect = 7.470e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query:  133 EPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            EP+ +      NN+  KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+   WL+ AY
Sbjct:  131 EPIKSVRRRQKNNSS-KQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVYRCRQSINFSLQCAWLLGAY 239          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 478.789 bits (1231), Expect = 9.362e-156
Identity = 257/502 (51.20%), Postives = 322/502 (64.14%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S   L++YF+ + G  N+E FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  341 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETQIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFRKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLKQLQIIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLLLLDYFRQEHGNFNTEEFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 834          

HSP 2 Score: 96.2857 bits (238), Expect = 7.470e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query:  133 EPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            EP+ +      NN+  KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+   WL+ AY
Sbjct:  131 EPIKSVRRRQKNNSS-KQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVYRCRQSINFSLQCAWLLGAY 239          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 478.789 bits (1231), Expect = 1.351e-155
Identity = 257/502 (51.20%), Postives = 322/502 (64.14%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S   L++YF+ + G  N+E FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  356 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETQIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFRKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLKQLQIIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLLLLDYFRQEHGNFNTEEFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 849          

HSP 2 Score: 96.2857 bits (238), Expect = 7.594e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query:  133 EPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            EP+ +      NN+  KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+   WL+ AY
Sbjct:  131 EPIKSVRRRQKNNSS-KQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVYRCRQSINFSLQCAWLLGAY 239          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 478.789 bits (1231), Expect = 1.351e-155
Identity = 257/502 (51.20%), Postives = 322/502 (64.14%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S   L++YF+ + G  N+E FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  356 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETQIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFRKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLKQLQIIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLLLLDYFRQEHGNFNTEEFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 849          

HSP 2 Score: 96.2857 bits (238), Expect = 7.594e-20
Identity = 45/110 (40.91%), Postives = 68/110 (61.82%), Query Frame = 1
Query:  133 EPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            EP+ +      NN+  KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+   WL+ AY
Sbjct:  131 EPIKSVRRRQKNNSS-KQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVYRCRQSINFSLQCAWLLGAY 239          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Match: cobl (cordon-bleu WH2 repeat protein [Source:Xenbase;Acc:XB-GENE-490658])

HSP 1 Score: 163.31 bits (412), Expect = 2.540e-40
Identity = 103/278 (37.05%), Postives = 160/278 (57.55%), Query Frame = 1
Query: 1744 IKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            I ES   G        + I K GDD RQ+ +   +L + Q V L     L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF  Q+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD +GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF    TE +    +  +++N   S  +R YD  QYY N I
Sbjct: 1790 IDESEEEGGDGKVCWQAAIFKVGDDCRQDLVV--LLNIFQLVGLD----LFVFPYRVVATAPGCGVIECIPDCTSRDQLGR-QTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2054          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Match: cobl (cordon-bleu WH2 repeat protein [Source:Xenbase;Acc:XB-GENE-490658])

HSP 1 Score: 163.31 bits (412), Expect = 2.699e-40
Identity = 103/278 (37.05%), Postives = 160/278 (57.55%), Query Frame = 1
Query: 1744 IKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            I ES   G        + I K GDD RQ+ +   +L + Q V L     L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF  Q+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD +GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF    TE +    +  +++N   S  +R YD  QYY N I
Sbjct: 1789 IDESEEEGGDGKVCWQAAIFKVGDDCRQDLVV--LLNIFQLVGLD----LFVFPYRVVATAPGCGVIECIPDCTSRDQLGR-QTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2053          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Match: cobl (cordon-bleu WH2 repeat protein [Source:Xenbase;Acc:XB-GENE-490658])

HSP 1 Score: 163.31 bits (412), Expect = 2.743e-40
Identity = 103/278 (37.05%), Postives = 160/278 (57.55%), Query Frame = 1
Query: 1744 IKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            I ES   G        + I K GDD RQ+ +   +L + Q V L     L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF  Q+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD +GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF    TE +    +  +++N   S  +R YD  QYY N I
Sbjct: 1822 IDESEEEGGDGKVCWQAAIFKVGDDCRQDLVV--LLNIFQLVGLD----LFVFPYRVVATAPGCGVIECIPDCTSRDQLGR-QTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2086          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Match: cobl (cordon-bleu WH2 repeat protein [Source:Xenbase;Acc:XB-GENE-490658])

HSP 1 Score: 163.31 bits (412), Expect = 2.878e-40
Identity = 103/278 (37.05%), Postives = 160/278 (57.55%), Query Frame = 1
Query: 1744 IKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            I ES   G        + I K GDD RQ+ +   +L + Q V L     L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF  Q+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD +GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF    TE +    +  +++N   S  +R YD  QYY N I
Sbjct: 1765 IDESEEEGGDGKVCWQAAIFKVGDDCRQDLVV--LLNIFQLVGLD----LFVFPYRVVATAPGCGVIECIPDCTSRDQLGR-QTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2029          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Match: cobl (cordon-bleu WH2 repeat protein [Source:Xenbase;Acc:XB-GENE-490658])

HSP 1 Score: 163.31 bits (412), Expect = 2.883e-40
Identity = 103/278 (37.05%), Postives = 160/278 (57.55%), Query Frame = 1
Query: 1744 IKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            I ES   G        + I K GDD RQ+ +   +L + Q V L     L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF  Q+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD +GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF    TE +    +  +++N   S  +R YD  QYY N I
Sbjct: 1811 IDESEEEGGDGKVCWQAAIFKVGDDCRQDLVV--LLNIFQLVGLD----LFVFPYRVVATAPGCGVIECIPDCTSRDQLGR-QTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDI 2075          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Match: Pi4kb (phosphatidylinositol 4-kinase beta [Source:MGI Symbol;Acc:MGI:1334433])

HSP 1 Score: 484.182 bits (1245), Expect = 2.921e-158
Identity = 271/569 (47.63%), Postives = 347/569 (60.98%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDL---VLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL    P     L  + ++H  S S    + S       LSS+L     +P +E +++       L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPTLSPAPDTGLSPSKRTHQRSKSDATASIS-------LSSNLKRTASNPKVENEDE----PVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 800          

HSP 2 Score: 84.3445 bits (207), Expect = 3.373e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Match: Pi4kb (phosphatidylinositol 4-kinase beta [Source:MGI Symbol;Acc:MGI:1334433])

HSP 1 Score: 483.026 bits (1242), Expect = 1.226e-157
Identity = 270/573 (47.12%), Postives = 344/573 (60.03%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL    P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPTLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815          

HSP 2 Score: 84.3445 bits (207), Expect = 3.480e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Match: Pi4kb (phosphatidylinositol 4-kinase beta [Source:MGI Symbol;Acc:MGI:1334433])

HSP 1 Score: 483.026 bits (1242), Expect = 1.672e-157
Identity = 270/573 (47.12%), Postives = 344/573 (60.03%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL    P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  263 KRELPTLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 827          

HSP 2 Score: 84.3445 bits (207), Expect = 3.704e-16
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  133 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 231          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Match: Pi4kb (phosphatidylinositol 4-kinase beta [Source:MGI Symbol;Acc:MGI:1334433])

HSP 1 Score: 469.159 bits (1206), Expect = 8.826e-157
Identity = 252/491 (51.32%), Postives = 317/491 (64.56%), Query Frame = 1
Query: 1216 VSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    E   + +          N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:    1 MAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 483          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Match: Pi4kb (phosphatidylinositol 4-kinase beta [Source:MGI Symbol;Acc:MGI:1334433])

HSP 1 Score: 261.922 bits (668), Expect = 3.978e-81
Identity = 132/230 (57.39%), Postives = 167/230 (72.61%), Query Frame = 1
Query: 1945 EPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDS--------------------ISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            EPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+                      I  +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:    1 EPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGCRRCSGASLSGPVVTVAQVICSQLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 226          
BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Match: sp|A4IID4|PI4KB_XENTR (Phosphatidylinositol 4-kinase beta OS=Xenopus tropicalis OX=8364 GN=pi4kb PE=2 SV=1)

HSP 1 Score: 482.641 bits (1241), Expect = 8.145e-157
Identity = 261/503 (51.89%), Postives = 324/503 (64.41%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELI----------QKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N F   L+            + +    E   + +          N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG   NW LLSVIVK GDDLRQE LAYQ+LK LQ +W SER PLW+RP  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMD+++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  312 LAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECEN-FETSLVPVRIPENRIRSTRSVENLPECGITHEQRASSFTTVPNYDNDDEAWSVDDIGELQVELPELHTNSCDNISQFSVDSITSQESKDPVFIAAGDIRRRLSEQL---AHTPTTFRRDPEDPSAVALKEPWQEKVRRIREGSPYGHFPNWRLLSVIVKCGDDLRQELLAYQVLKQLQSIWESERVPLWIRPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSCTTEAFLTAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDRVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNMTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 805          

HSP 2 Score: 87.0409 bits (214), Expect = 3.713e-16
Identity = 39/98 (39.80%), Postives = 60/98 (61.22%), Query Frame = 1
Query:  169 NTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            N   KQ  LLRLF+  +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+   WL+ AY
Sbjct:  127 NNSAKQSWLLRLFECKLFDVSMAISYLYNSKEPGVQAYIGNRLFCFRYEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVHRCRQSINFSLQCAWLLGAY 224          
BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Match: sp|B1MTG7|PI4KB_PLEMO (Phosphatidylinositol 4-kinase beta OS=Plecturocebus moloch OX=9523 GN=PI4KB PE=3 SV=1)

HSP 1 Score: 483.026 bits (1242), Expect = 8.397e-157
Identity = 270/573 (47.12%), Postives = 345/573 (60.21%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815          

HSP 2 Score: 86.6557 bits (213), Expect = 4.688e-16
Identity = 40/99 (40.40%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  YRC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVYRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Match: sp|Q8BKC8|PI4KB_MOUSE (Phosphatidylinositol 4-kinase beta OS=Mus musculus OX=10090 GN=Pi4kb PE=1 SV=2)

HSP 1 Score: 483.026 bits (1242), Expect = 8.579e-157
Identity = 270/573 (47.12%), Postives = 344/573 (60.03%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL    P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPTLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815          

HSP 2 Score: 84.3445 bits (207), Expect = 2.434e-15
Identity = 39/99 (39.39%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFYFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Match: sp|B4UT09|PI4KB_OTOGA (Phosphatidylinositol 4-kinase beta OS=Otolemur garnettii OX=30611 GN=PI4KB PE=3 SV=1)

HSP 1 Score: 483.026 bits (1242), Expect = 8.764e-157
Identity = 270/573 (47.12%), Postives = 345/573 (60.21%), Query Frame = 1
Query:  991 RKELGNFRPVSTLSLPANCKSHSSSYSTFNCN-----NSTKLHSEPL--SSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++EL +  P     L  + ++H  S S    +     N  +  S P   + D  L    E  +    +   L P+  FIK L++I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VIS+DSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL+AQ+ FV+SCA Y LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  251 KRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQL---AHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGI 815          

HSP 2 Score: 83.9593 bits (206), Expect = 3.706e-15
Identity = 38/99 (38.38%), Postives = 60/99 (60.61%), Query Frame = 1
Query:  166 NNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
             N   KQ  LLRLF+S +FD+ +A+ YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+    L+ AY
Sbjct:  121 QNNSAKQSWLLRLFESKLFDISMAMSYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAY 219          
BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Match: sp|Q6GN16|PI4KB_XENLA (Phosphatidylinositol 4-kinase beta OS=Xenopus laevis OX=8355 GN=pi4kb PE=2 SV=1)

HSP 1 Score: 482.256 bits (1240), Expect = 1.082e-156
Identity = 263/503 (52.29%), Postives = 322/503 (64.02%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELI----------QKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L P+  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P  G  H+VVR+P   AV LNSK +APYLIYVEV+   N F   L+            + +    E   + +          N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T    D  DPS+  L EPW++KV+RI+E SPYG   NW LLSVIVK GDDLRQE LA Q+LK LQ +W SER PLW+RP  I+VIS DSGMIEPV+NAVSIHQ+K+  S  SL+ YF  + G   +E+FL AQ+ FV+SCAAY LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHMDK++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQILV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  310 LAPEREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECEN-FETSLVPVRIPENRIRSTRSVENLPECGITHEQRASSFTTVPNYDNDDEAWSVDDIGELQVELPELHTNSCDNISQFSVDSITSQESKDPVFIAAGDIRRRLSEQL---AHTPTTFRRDPEDPSAVALKEPWEEKVRRIREGSPYGHFPNWRLLSVIVKCGDDLRQELLASQVLKQLQSIWESERVPLWIRPYKILVISGDSGMIEPVVNAVSIHQVKK-QSQLSLLHYFLQEHGSCTTEAFLTAQRNFVQSCAAYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTAEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQVVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMNMTEEQLQILVEQMVDGSMRSITTKLYDGFQYLTNGI 803          

HSP 2 Score: 87.0409 bits (214), Expect = 3.580e-16
Identity = 39/98 (39.80%), Postives = 60/98 (61.22%), Query Frame = 1
Query:  169 NTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            N   KQ  LLRLF+  +FD+ +AI YL    E G+QA++ N+ F +    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+   WL+ AY
Sbjct:  125 NNSAKQSWLLRLFECKLFDVSMAISYLYNSKEPGVQAYIGNRLFCFRYEDVDFYLPQLLNMYIHMDEDVGDAIKPYVVHRCRQSINFSLQCAWLLGAY 222          
BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Match: A0A1E1X2D8 (Putative phosphatidylinositol 4-kinase beta OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 573.933 bits (1478), Expect = 0.000e+0
Identity = 344/864 (39.81%), Postives = 475/864 (54.98%), Query Frame = 1
Query:  124 DVQEPVNNFESMCTNNTCEK---QEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHV---KKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGAS--HYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXX--XXNPGNIKEILDTDESGEEFNGET------------------------------------FVAAGDIRRRLKENIFLQKHQETVKVN-DDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            +V  PV+  E        E+   ++ LLRLF+S++FDM LAI YL +  E G+ A+L N+ FS+  + VDF+LPQ+V ++V +  ++D  + Y   RC +  ++S+ LVWL+ A+ +   A     KRS         A L+H+   + FR +I  + +S +       LT+         + K H+RS SD +G            MS      + ES R   L                                          L DL SGHAFDN C     +S   I G                              +    T++                   +C C +  L  Q  FIK+L+S+ +RL   P+K  KT  L+AEL+ +NLNLPARVW+P++  +  H VVRIPP AAV LNSK +APYLIYVEVVA ++      IQ  P+            S     D     P ++  +   D+  E +  E                                     FV A D+RRRL EN  + K+  TVK + +DPS++ L EPW++KVQRI+ESSPY  +  W L + IVK GDDLRQE +AYQ+LK+L+ +W  ER  LW+RP  I+V S+DSG++EP+L+ +S+HQ+K+ +S  SL++YF+ QFGP  SE+FL+AQ+CFVESCAAY L+ YL+QVKDRHNGNILLD EGH+IHID+GFILS+SP +NLGFE SPFKLT E VD+MGG+ S+MF YFK+LILQGL+AARKH ++I+  +EIL S + +LPCF     +     R+RFH+  T+ +LQ+LVDS+VE+S+HSLTTRLYD+FQY+TNGI
Sbjct:  153 EVHSPVSPQEETVQTPAAERLVPKDLLLRLFESTLFDMPLAIDYLFRSKEPGVLAYLGNRMFSFSDADVDFYLPQIVSMYVHHSDIADSLRPYLVQRCSKDTDFSLQLVWLLSAFCSDPNAPPR--KRSQ-------GAQLKHLILSEDFRPRITVQQTSSL-------LTVS--------SKKTHQRSLSDATG------------MSGGGGRHIPESSRKAAL------------------------------------------LGDLGSGHAFDNGCTC--HDSCDGIVG------------------------------DLRGRTRV-------------------ECHCQAPRLTSQNQFIKVLISLGLRLQAVPTKELKTQRLQAELSLVNLNLPARVWLPLHSDTFPHLVVRIPPQAAVVLNSKDKAPYLIYVEVVAVDD------IQTSPVPAKVLNPLRLTRSEENLPDYFGVQPASVFSMSGMDDDAECWTQEDDEISMQFLRNQKAQERDTLSVLSLDSGTSADSKEPLFVVASDVRRRLSENSHVPKN--TVKRDPEDPSAAALKEPWEEKVQRIRESSPYSHLPGWQLRAAIVKCGDDLRQELMAYQLLKLLKTIWQQERVSLWLRPYRILVTSADSGLVEPILDTMSLHQVKK-HSQLSLLQYFEQQFGPPTSEAFLSAQRCFVESCAAYCLISYLIQVKDRHNGNILLDGEGHIIHIDFGFILSSSP-KNLGFESSPFKLTQEFVDVMGGLDSNMFNYFKVLILQGLVAARKHHERIITLVEILQS-NARLPCFQWHGASAVRALRERFHMGSTDERLQMLVDSLVESSMHSLTTRLYDNFQYFTNGI 876          
BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Match: A0A2R5LNV9 (Putative phosphatidylinositol 4-kinase (Fragment) OS=Ornithodoros turicata OX=34597 PE=3 SV=1)

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 336/836 (40.19%), Postives = 451/836 (53.95%), Query Frame = 1
Query:  193 LLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIY--GASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXX--XXNPGNIKEILDTDESGEEFNGE------------------------------------TFVAAGDIRRRLKENIFLQKHQETVKVN-DDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGIQ 2577
            LLRLF S +FDM +AI YL    E G+  ++ N+ FS+  S VDF+LPQLV ++V +  +++    Y   RC++   +S+ L WL+ A+ +   A +   KRS      N I + E   +   Q++T   +F        LT+           K H+RS SD +G+         +Q S            N  L Y  G                    N +R           N+L DL SGHAFDN C  ++                            C S  S +       S +L                   +C C +  L PQ  FIK+L+S+ +RL    +K +KT  L+AEL+ +N+NLPARVW+PI+     H VVR+PP AAV LNSK +APYLIYVEVV   +      IQ  P+            S     +      G++  +   D+  E +  E                                     FVAAGDIRRRL +N+ + K    VK + +DPS++ L EPW++KV+RI+E+SPY  +  W LL+ IVK GDDLRQE + YQ+L +LQK+W  E  PLWVRP  I+V S DSG++EP+LN +S+HQ+K+  S  SL+EYF  +FG   +E FL AQ+ FV SCAAY LV YL+QVKDRHNGNILLD EGH+IHID+GFILS+SP +NLGFE SPFKLT E VD+MGG+ SDMFEYFKIL+LQGL+AARKH ++IL  +EIL + + +LPCF +         R+R HL   E +L +LV+S+VE+S+HSLTTRLYD FQYYTNGI 
Sbjct:  125 LLRLFTSELFDMPIAINYLFFSKEPGVITYIGNRMFSFKDSDVDFYLPQLVSMYVHHSEVAEALHPYLVQRCRKDANFSLQLCWLLSAFCSDPNAPSR--KRSQGIKLKNLILSEELRPRVPLQLVT--PTFNGSAMGPSLTVA--------TKKTHQRSLSDATGM---------MQPS------------NSGLRYLGG-------------------MNASRK----------NALGDLNSGHAFDNGCTCYD---------------------------TCDSFVSDWR----GGSGRL-------------------ECHCQAPRLSPQNHFIKVLISLGLRLQTVSTKDRKTQRLQAELSMVNMNLPARVWLPIHLDTLPHLVVRVPPQAAVVLNSKDKAPYLIYVEVVEVAD------IQTSPVPAKIINTLRQTRSEENLPEYFGQTAGSVFSMCSMDDDPECWTQEDDEISLQFLRAHKMQDRDTLSVLSLDSGTSADSKEPVFVAAGDIRRRLSDNLHVPKC--IVKRDPEDPSAAALKEPWEEKVRRIREASPYSHLPGWRLLAAIVKCGDDLRQELMVYQLLSVLQKIWEQEHVPLWVRPYRILVTSEDSGLVEPILNTMSLHQVKKHSSQLSLLEYFVQEFGAPTTEGFLCAQRNFVHSCAAYCLVSYLVQVKDRHNGNILLDGEGHIIHIDFGFILSSSP-KNLGFENSPFKLTQEFVDVMGGMGSDMFEYFKILMLQGLVAARKHHERILTLVEILQA-NARLPCFQRHGALALRTLRERLHLGIPEEKLHLLVESLVESSMHSLTTRLYDGFQYYTNGIH 838          
BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Match: A0A069DX32 (Putative phosphatidylinositol 4-kinase (Fragment) OS=Panstrongylus megistus OX=65343 PE=2 SV=1)

HSP 1 Score: 545.428 bits (1404), Expect = 1.258e-179
Identity = 329/827 (39.78%), Postives = 449/827 (54.29%), Query Frame = 1
Query:  193 LLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKF-------RNQIITENSSFIHLICEN-RLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKK--PIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGEEFNG----------------------ETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            LLRLF+S +FDM +AI YL    E G+Q+++ NK FS+P + VDF+LPQLV +++  P +++V   Y  +RC+ES+++S+   WL+DAY +   A+++  K     L    ++     +++       R Q++  N+      C N      +S        K H RS SD S L +      SL               NG    C G                                       DL SG AFDN C  F+     +  G   +L   R                   T  C ++ +L  E                            L  IK L++I   L    +K  +T  L +EL  +NLNLP+RVW+PI     H++VRIPP+AA  LNSK +APY++Y+EVV   +L    ++ K    +  +  + + + +         +  +     D DE  +++ G                       +  AAGDIRRRL E+  L   +      +DPS++ L EPW++K +RI+E SPYG + +W LLSVIVK GDDLRQE LA Q+L MLQ +W  ER PLW+ P  I+ +S DSG+IEP+LN VS+HQIK+ +   SL EYF  +FGPTNSE+FL AQ+ FV SCAAY LV YL+QVKDRHNGNILL  EGH+IHID+GFILS SP RNLGFE SPFKLT E VD+MGG KSDMFEYFKIL+LQGL+AARKHMDKIL+ +EI+  +  +LPCF  G   V    ++RFH+  TE QLQ+L+D++VE+S+HS++T+LYD FQY TNGI
Sbjct:   15 LLRLFESKLFDMSMAIMYLFNSKEPGVQSYIGNKVFSFPDNEVDFYLPQLVNMYIQMPDVAEVLHPYLVHRCRESVDFSLKCSWLLDAYSSD-AALSSKKKSHGTKLKNLILSGELRPREYEPTRRVSRPQVMVPNA------CTNGTCAPTNSLAAPSPLKKTHHRSRSDASALHQGQQKAISL---------------NGTSRVCLG---------------------------------------DLNSGRAFDNGCTCFD-----SCQGKVNDLRGKR-------------------TVCCCDAPRLTPE----------------------------LELIKTLINIGKTLATVGTKEARTTRLISELTLVNLNLPSRVWLPINSHTPHHIVRIPPHAAAVLNSKDKAPYILYIEVVEVEDLATSPVVSKSMNTLRHTKSEENLAAALTSYSHLELDAESDCWSQDDDEISQQYVGVRKTVERDTISQLSQESTDSREPSLFAAGDIRRRLSES--LHDPRPFTHDPEDPSAAALKEPWEEKERRIRECSPYGYLTSWRLLSVIVKCGDDLRQELLASQVLTMLQNIWTVERVPLWLHPYRILCLSDDSGLIEPILNTVSLHQIKK-HCQLSLGEYFAKEFGPTNSEAFLTAQRNFVRSCAAYCLVSYLVQVKDRHNGNILLSGEGHLIHIDFGFILSTSP-RNLGFETSPFKLTSEFVDVMGGPKSDMFEYFKILMLQGLVAARKHMDKILSLVEIM-RLGSQLPCFKCGAAAV-QGLKNRFHMNMTEEQLQLLIDNLVESSIHSISTKLYDGFQYLTNGI 722          
BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Match: F1KUT5 (Phosphatidylinositol 4-kinase beta OS=Ascaris suum OX=6253 PE=2 SV=1)

HSP 1 Score: 530.02 bits (1364), Expect = 3.087e-171
Identity = 323/832 (38.82%), Postives = 459/832 (55.17%), Query Frame = 1
Query:  193 LLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYR--NYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGAS--HYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDES----------------GEEFN-----GETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYS-------------GKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            LLRLF+S +FD+ +A+QYLS   E G+  +L NK F +P  SVDF++PQLV L++    ++DV   Y   RC+ES+E+S+   WL++AY  G  A+    +++             H  + R  I+ E   F + +      I      ++R  + + H RS S+ + +  +++ N+S      NN    E  R+G +                        +N +          ++ S  DL +G AFDN C  F+     N     KE          ++   CK                                       C +  + P+  F+K L+++  RL   P K  K+ HL  EL  +NLNLPARVW+P+Y  +  H V+RIP +A   LNSK +APY +YVEV+  ++      ++   +      +++++    +    +N  N   + D  ++                 EE +      E  ++A +IR+RL  N   +  ++   V DDPS+S + EPW++K  RI+E+SPYG+   W LL VIVK GDDLRQE LAYQ+L  L+ +W+ E+ PL++RP  IVV S +SGMIEP+LNA S+HQIK+  +               +L+ +F   FGP+N ESFL AQ+ FV+SCA YSL CY LQVKDRHNGNILLD+EGH+IHID+GFILS SP RNLGFE SPFKLT E VD+MGGV SDMF+Y+KIL+LQGL+AARKH D+I+  +EI+ + S +LPCF  G  TV  + R+RFH+  TE+QLQ LVD+MVENSL ++TTRLYD+FQYYTNGI
Sbjct:  201 LLRLFESKLFDVVIAMQYLSSSKEPGVLHYLGNKLFDFPVESVDFYVPQLVNLYINMREVADVIYPYMVKRCRESVEFSLECCWLLEAY--GVDAMRKQKRKA-------------HGYRLRRLILNE---FRNELEPGESAITAPPAPLFRASSVRAHSRSRSEAT-VRGLDATNESATKLVGNNG---ELRRSGSV------------------------FNTS----------TLTSFGDLTTGRAFDNGCVCFDDTMVDNA----KE----------AMKMECK---------------------------------------CGAQRIRPEQEFVKALMNVGNRLKEIPLKEDKSRHLVNELIMINLNLPARVWLPLYADTVRHVVLRIPHSAGCVLNSKDKAPYCLYVEVLEVDD------VRTSFVPHRISDIEAAEFQRKERQGSANSLNSPSLTDDSQTPPSSVAATSVDSVSRFSEERSPLADPAERIISAAEIRKRLT-NWVKKPRKQLRHVPDDPSASEMSEPWEEKQARIREASPYGRNPKWRLLPVIVKTGDDLRQELLAYQLLTTLKNIWIEEKVPLYLRPYKIVVCSQNSGMIEPILNASSLHQIKKNLTISANEQLDPERRLPPTLLTHFLESFGPSNCESFLIAQQNFVQSCAGYSLACYFLQVKDRHNGNILLDSEGHLIHIDFGFILSISP-RNLGFETSPFKLTQEIVDVMGGVGSDMFKYYKILLLQGLIAARKHHDRIVNIVEIMLAGS-QLPCFRGGANTV-RSLRERFHMNCTESQLQALVDTMVENSLGAITTRLYDNFQYYTNGI 913          
BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Match: A0A0A9YXT1 (Phosphatidylinositol 4-kinase beta (Fragment) OS=Lygus hesperus OX=30085 GN=PI4KB_1 PE=3 SV=1)

HSP 1 Score: 526.939 bits (1356), Expect = 5.502e-171
Identity = 329/837 (39.31%), Postives = 445/837 (53.17%), Query Frame = 1
Query:  136 PVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYG-ASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKK-----------------------PIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            P+   E      +   +  LLRLF+S +FDM +AI YL    E G+Q+++ NK FS+  + VDF+LPQ+V +++  P +++V   Y  +RC+ES+++S+   WL+DAY +   A  +  K+S  +   N I + E   + R+   T   S    I  +  ++   +       K H RS SD S L                  L  +S  NG    C G                                       DL SG A+DN C  F+        G   +L   R + +   P                               L P +EL          +   ++  K+L +IS       +K  +T  L +EL  +NLNLPARVW+P+     H++VRIPP+AA  LNSK +APY++YVE V   +L    ++ K                         +               Q +  + P     I    +  +      F A GDIRRRL E+  L   +      +DPS++ L EPW++K +RI+E SPYG + +W LLSVIVK GDDLRQE LA QIL MLQ +W  ER PL + P  I+V+S DSG+IEP+LN VS+HQIK+ +   SL +YF  +FG  NSE FLNAQ+ FV SCAAY LV YL+QVKDRHNGNILL+NEGH+IHID+GFILS SP RNLGFE SPFKLT E V++MGG +SDMFEYFKIL+LQGL+AARKHMDKI+  +EI+  +  +LPCF  G  TV    ++RFHL KTE QLQ+LVD+MVE+S+HSL+T +YD FQY+TNGI
Sbjct:  124 PIRTLEQSLPPPSQSSKGFLLRLFESKLFDMSMAITYLFNSKEPGVQSYIGNKIFSFLDNEVDFYLPQMVNMYIQMPDVAEVLHPYLVHRCRESVDFSLKCSWLLDAYSSD--AALSSKKKSHGTKLKNLILSGE--LRPRDSEPTRRVSRPSAITSSPTSLTAPSP----LKKTHHRSRSDASAL------------------LGNKSSSNGTSRVCLG---------------------------------------DLNSGRAYDNGCTCFD-----TCQGKVNDLRGKRTLCSCDAPR------------------------------LSPELEL----------IKSLINIGKLLATIS-------TKEARTTRLISELTTVNLNLPARVWLPLSTHIPHHIVRIPPHAAAVLNSKDKAPYILYVEAVEVEDLASSPIVSKSMNTLRHTKSEENLAAAHSSYSHLELEEMDAWSQEEDDISQQYLQLNKPVERDSISQLSQESDSKESSIFAA-GDIRRRLSES--LHDPRPFTHDPEDPSAAALKEPWEEKERRIRECSPYGYLNSWKLLSVIVKCGDDLRQELLASQILTMLQSIWEIERVPLQLHPYRILVLSDDSGLIEPILNTVSLHQIKK-HCNLSLYKYFVKEFGAPNSEGFLNAQRNFVRSCAAYCLVSYLIQVKDRHNGNILLNNEGHLIHIDFGFILSTSP-RNLGFETSPFKLTPEFVEVMGGPESDMFEYFKILMLQGLVAARKHMDKIIGIVEIM-RLGSQLPCFKSGAATV-QGLKNRFHLNKTEEQLQLLVDNMVESSIHSLSTMIYDGFQYFTNGI 836          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Match: pi4kb (phosphatidylinositol 4-kinase beta [Source:NCBI gene;Acc:103035584])

HSP 1 Score: 477.633 bits (1228), Expect = 2.051e-155
Identity = 256/502 (51.00%), Postives = 321/502 (63.94%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG   DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  338 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETRIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFKKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGNYTTEAFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTNEFVDVMGGQDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 831          

HSP 2 Score: 92.0485 bits (227), Expect = 1.380e-18
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 1
Query:  169 NTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAY-PHLSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+   WL+ AY
Sbjct:  152 NNTSKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEEVGDAIKPYVVHRCRQSINFSLQCAWLLGAY 249          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Match: pi4kb (phosphatidylinositol 4-kinase beta [Source:NCBI gene;Acc:103035584])

HSP 1 Score: 477.633 bits (1228), Expect = 2.952e-155
Identity = 256/502 (51.00%), Postives = 321/502 (63.94%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP---------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETV--KVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P         +    +   +            N  N  E    D+ GE                           E     F+AAGDIRRRL E +    H  T   K  +DPS+  L EPW +KV+RI+E SPYG + NW LLSVIVK GDDLRQE LA+Q+LK LQ +W  ER PLW++P  I+VISSDSGMIEPV+NAVSIHQ+K+  S  SL++YF  + G   +E+FL AQ+ FV+SCA Y L+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG   DMF Y+K+L+LQGL+AARKHM+K++  +EI+   S +LPCF  G  T+  N ++RFH+  TE QLQ+LV+ MV+ S+ S+TT+LYD FQY TNGI
Sbjct:  351 LTPQREFIKSLMGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPETRIRSTRSVENLPDCGITPDQRASSFSTVPNYDNDDEAWSVDDIGELQVELPEIHTNSCDNISQFSVDSITSQESKEPIFIAAGDIRRRLSEQL---AHTPTTFKKDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISSDSGMIEPVVNAVSIHQVKK-QSQLSLLDYFLQEHGNYTTEAFLTAQRNFVQSCAGYCLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-RNLGFETSAFKLTNEFVDVMGGQDGDMFNYYKMLMLQGLIAARKHMEKVIQIVEIMQQGS-QLPCF-HGSSTI-RNLKERFHMSLTEEQLQVLVEQMVDGSMRSITTKLYDGFQYLTNGI 844          

HSP 2 Score: 92.0485 bits (227), Expect = 1.472e-18
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 1
Query:  169 NTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAY-PHLSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            N   KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+   WL+ AY
Sbjct:  152 NNTSKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFSFRNEDVDFYLPQLLNMYIHMDEEVGDAIKPYVVHRCRQSINFSLQCAWLLGAY 249          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Match: pi4kab (phosphatidylinositol 4-kinase, catalytic, alpha b [Source:ZFIN;Acc:ZDB-GENE-080220-34])

HSP 1 Score: 174.866 bits (442), Expect = 3.094e-44
Identity = 98/262 (37.40%), Postives = 162/262 (61.83%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD RQ+ LA QI+ + + ++      L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF+NQ+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD++GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF+   +E +    +  +++N   S  ++ YD  QYY N I
Sbjct: 1476 AAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGR-QTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSQGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFNPSMSEKEAAAFMIKVIQNCFLSSRSKTYDMIQYYQNQI 1730          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Match: pi4kaa (phosphatidylinositol 4-kinase, catalytic, alpha a [Source:ZFIN;Acc:ZDB-GENE-100204-2])

HSP 1 Score: 173.711 bits (439), Expect = 8.260e-44
Identity = 102/281 (36.30%), Postives = 169/281 (60.14%), Query Frame = 1
Query: 1735 VQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            V+  +E+S   +   W   + I K GDD RQ+ LA QI+ + + ++      L+V P  +V  +   G+IE + +  S  Q+ R  +   + +YF+NQ+G  ++ +F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD++GH+IHID+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA R +MD +++ + ++  +   LPCF +G +T+    + RF+   +E +    +  ++E    S  ++ YD  QYY N I
Sbjct: 1743 VEDGEENSEAAQKVCWQ--AAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCKSRDQLGR-QTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSKGHLIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLM--LDTGLPCF-RG-QTI-KLLKQRFNPNMSEKEAAAFMIKVIERCFLSSRSKTYDMLQYYQNQI 2014          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Match: si:rp71-17i16.5 (phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform-like [Source:NCBI gene;Acc:103035431])

HSP 1 Score: 107.071 bits (266), Expect = 3.292e-23
Identity = 72/224 (32.14%), Postives = 111/224 (49.55%), Query Frame = 1
Query: 1789 LSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKS-------LIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPG-RNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVY 2436
            + +I K GDDLRQ+ L  Q L ++  +W      L + P   +    + GMIE V +AV+I  I+R   G +       L E+ + +  P   + +   +K FV SCAAY +  Y+L + DRHN NI++ N+G++ HID+G IL  +     +  E  PF LT + + +MG VK +   YF+   +   L   K    +  T +    I  K+    KG   VY
Sbjct:  818 VGIIFKQGDDLRQDMLIIQTLMVMDSIWQENCLDLNLVPYGCISTGYNIGMIEIVRDAVTIAAIQRNQGGTTGAFKNNALYEWLREK-CPLLEKQYQAMEK-FVNSCAAYCVATYVLGIGDRHNDNIMITNQGNLFHIDFGHILGNTKSFLGVNRERVPFVLTPDFLYVMGRVKGESSLYFRRFKVSISLNHEKKGSSLKTTYDTYTHIFLKVTQNEKGLSVVY 1039          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 469.159 bits (1206), Expect = 5.114e-154
Identity = 253/494 (51.21%), Postives = 326/494 (65.99%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP-----IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGEEFNGET-----------FVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKR---------LYSGK-----SLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L P+  FI+ L++I  RL   P+K QKT  L AEL+ LNL LPARVW+      H++VRIP    + LNSK +APYLIYVEV+  ++L    +  + P      + S E L    S  +     + P   ++  ++ ++  +F+ ++           ++ AGDIRRRL E + +   Q   K  +DPS++ L EPW +KV+RI+E SPYG   NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+V S+DSGMIEP++NAVSIHQ+ R          YS K     SL+EYF  + G   +E FL AQ+ FV+SCAAY LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS SP RNLGFE S FKLT E VD+MGG  SDMF YFK+L+LQGL+ ARKHM++++  +EI+   S +LPCF  G  T+    ++RFH+  TE QLQ  +D MVE S+ SLTTRLYD FQY+TNGI
Sbjct:  250 LAPEREFIQTLMAIGKRLVSLPTKEQKTQRLIAELSLLNLTLPARVWLATASFPHHIVRIPHTQTIVLNSKDKAPYLIYVEVLVCDDLERSVVPARIPEARIRTARSEECLQDESSPAVASETHTQPPETQKSNNSSDTISQFSLDSITSLDSKTEPVYILAGDIRRRLSEQLSVPITQ-FRKDPEDPSAAALKEPWKEKVRRIREVSPYGHFPNWRLLSVIVKCGDDLRQELLAYQVLKQLQMIWEQERVPLWLKPYKILVTSADSGMIEPIVNAVSIHQVPRPELTKHPNARYSVKKQSQLSLLEYFLQEHGSRTTEGFLTAQRNFVQSCAAYCLVCYLLQVKDRHNGNILLDLEGHIIHIDFGFILSNSP-RNLGFEVSAFKLTQEFVDVMGGQDSDMFIYFKLLMLQGLIGARKHMERVVQIVEIMQQGS-QLPCF-HGASTI-RQLKERFHMSLTEEQLQATIDQMVEGSMRSLTTRLYDGFQYFTNGI 738          

HSP 2 Score: 88.1965 bits (217), Expect = 4.139e-18
Identity = 38/93 (40.86%), Postives = 59/93 (63.44%), Query Frame = 1
Query:  181 KQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFV-AYPHLSDVFKSYFTYRCKESIEYSISLVWLMDA 456
            KQ  LLRLF+S +FD+ +AI YL    E G+QA+L N+ FS+P  + DF+LPQL+ +++       D  K Y  +RC+ S ++++   WL+ A
Sbjct:   24 KQSLLLRLFESKLFDVSMAISYLHSSKEPGVQAYLGNRLFSFPTEATDFYLPQLLTMYLHGSEETGDAVKPYLVHRCRRSPDFALRCAWLLGA 116          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 403.29 bits (1035), Expect = 2.497e-128
Identity = 198/319 (62.07%), Postives = 244/319 (76.49%), Query Frame = 1
Query: 1618 AGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            AGDIRRRL E + L   Q   +  +DPS++ L EPW +KV+RI+E+SPYG   NW LLSVIVK GDDLRQE LAYQ L+ LQ VW  ER PLW++P  I+V S+DSGMIEP+LNAVSIHQ+K+  S  SL+EYF  + G  N+E FL AQ+ FVESCAAY L+CY+LQVKDRHNGNILLD+EGH+IHID+GFILS SP RNLGFE S FKLT E V++MGG  SDMF Y+K+L+LQGL+AARKHM++++  +EI+   S +LPCF  G  T+    ++RFH+  TE QLQ  ++ MVE S+ SLTTRLYD FQY TNGI
Sbjct:  469 AGDIRRRLSEQLNLPATQ-FRRDPEDPSAAALKEPWKEKVRRIRETSPYGHFPNWKLLSVIVKCGDDLRQELLAYQFLRQLQSVWEQERVPLWLKPYKILVTSADSGMIEPILNAVSIHQVKK-QSQLSLLEYFLQEHGSPNTEGFLTAQRHFVESCAAYCLICYILQVKDRHNGNILLDSEGHIIHIDFGFILSNSP-RNLGFETSAFKLTAEFVEVMGGPDSDMFNYYKLLMLQGLIAARKHMERVVQIVEIMQQGS-QLPCF-HGASTI-RQLKERFHVSLTEEQLQSTIEQMVEGSMRSLTTRLYDGFQYITNGI 781          

HSP 2 Score: 98.9821 bits (245), Expect = 1.670e-21
Identity = 47/88 (53.41%), Postives = 63/88 (71.59%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNL 1446
            L P+  FIK L++I  RL   P++ QKT  L AEL+ LNL+LPARVW+P   ++H++VR+P   AV LNSK +APYLIYVEV+   +L
Sbjct:  282 LAPEREFIKSLMAIGKRLATLPTREQKTQRLIAELSLLNLSLPARVWLPTALSAHHIVRLPHTQAVVLNSKDKAPYLIYVEVLECEDL 369          

HSP 3 Score: 93.5893 bits (231), Expect = 7.528e-20
Identity = 59/201 (29.35%), Postives = 95/201 (47.26%), Query Frame = 1
Query:  169 NTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFV-AYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVK--KFRNQIITEN--------SSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLS-------GLSRVNSINK 717
            ++  KQ  LLRLF+S +FD+ +AI YL    E G+QA+L NK FS+P  + DF+LPQL+ +++       D  K Y  +RC+ S ++++   WL+ AY                S D    +  +H +  K R  I+++         S+  H +       K     +    + H+RS SD +       GL R +SI +
Sbjct:   72 SSASKQSLLLRLFESKLFDVSMAISYLHSSKEPGVQAYLGNKLFSFPADATDFYLPQLLTMYLHGAEEAGDAIKPYLVHRCRRSPDFALRCAWLLGAY----------------SADGQPSSRQKHSRGTKLRRLILSDELVPATPAPSAPPHQLLAASARAKLRAAAVSPGKRTHQRSKSDATSSCIQADGLRRTSSIPR 256          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Match: pi4kb (phosphatidylinositol 4-kinase, catalytic, beta [Source:NCBI gene;Acc:563201])

HSP 1 Score: 387.111 bits (993), Expect = 1.318e-124
Identity = 217/427 (50.82%), Postives = 270/427 (63.23%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEI-------LDTDESG---------------------EEFNGETFVA-----------AGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARK 2346
            L P+  FI+ L++I  RL   P+K QKT  L AEL+ LNL LPARVW+      H++VRIP    + LNSK +APYLIYVEV+  ++L    +  + P     E    +  S     D S+P    E+       LD DE                        +F+ ++  +           AGDIRRRL E + +   Q   K  +DPS++ L EPW +KV+RI+E SPYG   NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+V S+DSGMIEP++NAVSIHQ+K+  S  SL+EYF  + G   +E FL AQ+ FV+SCAAY LVCYLLQVKDRHNGNILLD EGH+IHID+GFILS SP RNLGFE S FKLT E VD+MGG  SDMF YFK+L+LQGL+ ARK
Sbjct:  161 LAPEREFIQTLMAIGKRLVSLPTKEQKTQRLIAELSLLNLTLPARVWLATASFPHHIVRIPHTQTIVLNSKDKAPYLIYVEVLVCDDLERSVVPARIP-----EARIRTARSEECLQDESSPAVASELRLCPLPTLDPDEEAWSQDDIADLQVEKSNNSSDTISQFSLDSITSLDSKTEPVYILAGDIRRRLSEQLSVPITQFR-KDPEDPSAAALKEPWKEKVRRIREVSPYGHFPNWRLLSVIVKCGDDLRQELLAYQVLKQLQMIWEQERVPLWLKPYKILVTSADSGMIEPIVNAVSIHQVKK-QSQLSLLEYFLQEHGSRTTEGFLTAQRNFVQSCAAYCLVCYLLQVKDRHNGNILLDLEGHIIHIDFGFILSNSP-RNLGFEVSAFKLTQEFVDVMGGQDSDMFIYFKLLMLQGLIGARK 579          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008978.1 (pep scaffold:Pmarinus_7.0:GL477391:15964:91650:-1 gene:ENSPMAG00000008040.1 transcript:ENSPMAT00000008978.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 168.318 bits (425), Expect = 8.657e-43
Identity = 101/266 (37.97%), Postives = 162/266 (60.90%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHI--DYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAA--RKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD RQ+ LA QI+ + + ++      L+V P  +V  S   G+IE + +  S  Q+ R  +   + EYF+NQ+G  ++E+F  A+  F+ S AAYSL+ +LLQ+KDRHNGNI+LD++GH+IHI  D+GF+  +SPG NLG+E    KLT E V IMGG +++  F++F  + ++G LA   R +MD +++ + ++  +   LPCF +G +T+    + RF   ++E      +  +++N   S  ++ YD  QYY N I
Sbjct: 1850 AAIFKVGDDCRQDMLALQIIGLFKNIFQLVGMDLFVFPYRVVATSPGCGVIECIPDCKSRDQLGR-QTDIGMYEYFRNQYGDESTEAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSQGHLIHIVLDFGFMFESSPGGNLGWE-PDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVSNRPYMDAVISLVALM--LDTGLPCF-RG-QTI-KLLKQRFAPNQSERDAANYMIKIIQNCFLSNRSKTYDMIQYYQNQI 2108          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Match: pik3c2a (phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha [Source:ZFIN;Acc:ZDB-GENE-030328-39])

HSP 1 Score: 102.449 bits (254), Expect = 1.974e-22
Identity = 75/261 (28.74%), Postives = 126/261 (48.28%), Query Frame = 1
Query: 1789 LSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSI------HQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASP--GRNLGFEFSPFKLTYEEVDIMGGVK--SDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRL 2541
            + V+ K GDDLRQ+ L  Q++K++ K+WL E   + +     +    + GM+E VL + ++      H +   +  K L E+ +    PT  E +  A + F+ SCA   +  Y+L + DRHN NI+L   GHM HID+G  L  +   G     + +PF LT +   ++ G +  +  F++F  L  Q     RKH +  L  + ++ S         +  + VY     +     TEA  Q     ++E+SL S+ T++
Sbjct:  391 IQVMFKVGDDLRQDMLTLQMIKIMDKIWLQEGLDMRMVLFKCISTGKERGMVELVLASDTLRKIQVEHGVTGSFKDKPLAEWLQKH-NPTEQE-YEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLRTTGHMFHIDFGKFLGHAQMFGNIKRCDRAPFVLTSDMAYVINGGERPTSRFQHFVDLCCQAYNLIRKHTNLFLNLLGLMMSSGIPELADVQDLKYVYEALMPQ--ATDTEATAQF--TRLIESSLGSMATKV 645          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Match: PIK1 (Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p [Source:SGD;Acc:S000005211])

HSP 1 Score: 268.085 bits (684), Expect = 2.298e-76
Identity = 132/306 (43.14%), Postives = 197/306 (64.38%), Query Frame = 1
Query: 1702 SSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGK--------------SLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDS-MVENSLHSLTTRLYDSFQYYTNGI 2574
            +S L E W  K +RI+++S YG  ENW+L SVI K GDDLRQE  AYQ+++ +  +W+ E+  +WV+ + I++ S+++G++E + NA+S+H IK+  + K              SL ++F   FG  N   +  AQ  F  S AAYS++CYLLQVKDRHNGNI++DNEGH+ HID+GF+LS SPG ++GFE +PFKLTYE ++++GGV+ + F+ F  L      A RK+ D+I++  EI+   + + PCF  G +T     R RF L  +E ++   V++ ++  SL S+ TR+YD FQ  T GI
Sbjct:  762 TSYLGEDWATKKERIRKTSEYGHFENWDLCSVIAKTGDDLRQEAFAYQMIQAMANIWVKEKVDVWVKRMKILITSANTGLVETITNAMSVHSIKKALTKKMIEDAELDDKGGIASLNDHFLRAFGNPNGFKYRRAQDNFASSLAAYSVICYLLQVKDRHNGNIMIDNEGHVSHIDFGFMLSNSPG-SVGFEAAPFKLTYEYIELLGGVEGEAFKKFVELTKSSFKALRKYADQIVSMCEIMQKDNMQ-PCFDAGEQTSV-QLRQRFQLDLSEKEVDDFVENFLIGKSLGSIYTRIYDQFQLITQGI 1064          

HSP 2 Score: 56.225 bits (134), Expect = 1.822e-8
Identity = 55/161 (34.16%), Postives = 78/161 (48.45%), Query Frame = 1
Query: 1024 TLSLPA--NCKSHSSSYSTFNCNNSTKLH---SEPLS---------SDLVLHPPIEL-------KNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIY------GASHYVVRIPPNAAVCLNSKMRAPYLIYVE 1425
            T S+P   N +  +SS S+ +   + KL+   S+PLS         ++  L   I+L       K K   A+YF   +  F   L +IS RL R P++  + + LRAEL  LN +LPA V IP        G  H +V I  N A  LNS  + PYL+ +E
Sbjct:  364 TSSMPDLHNIQPRTSSASSASLEGTPKLNRTNSQPLSRQAFKNSKKANSSLSQEIDLSQLSTTSKIKMLKANYFR-CETQFAIALETISQRLARVPTE-ARLSALRAELFLLNRDLPAEVDIPTLLPPNKKGKLHKLVTITANEAQVLNSAEKVPYLLLIE 522          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Match: STT4 (Phosphatidylinositol-4-kinase; functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization [Source:SGD;Acc:S000004296])

HSP 1 Score: 186.037 bits (471), Expect = 1.267e-48
Identity = 104/270 (38.52%), Postives = 161/270 (59.63%), Query Frame = 1
Query: 1768 KIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGV-KSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            ++E W   + I K GDD RQ+ LA Q++ + + +W S    ++V P  +   +   G+I+ + N+VS   + R  +   L EYF ++FG  ++  F NA+  FV+S A YS++ YLLQ KDRHNGNI+ D++GH +HID+GFI    PG  + FE  PFKLT E V +MGG  ++  +  F+ L ++  LAAR H++ I+  +  +  +   LPCF KG +T+  N R RF   KT+ +  + + +++  S  S+ T+ YD FQ  TNGI
Sbjct: 1637 EVEKWQ--AAIFKVGDDCRQDVLALQLISLFRTIWSSIGLDVYVFPYRVTATAPGCGVIDVLPNSVSRDMLGR-EAVNGLYEYFTSKFGNESTIEFQNARNNFVKSLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPG-GIKFEAVPFKLTKEMVKVMGGSPQTPAYLDFEELCIKAYLAARPHVEAIIECVNPM--LGSGLPCF-KGHKTI-RNLRARFQPQKTDHEAALYMKALIRKSYESIFTKGYDEFQRLTNGI 1898          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Match: VPS34 (Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery [Source:SGD;Acc:S000004230])

HSP 1 Score: 95.1301 bits (235), Expect = 2.105e-20
Identity = 69/250 (27.60%), Postives = 121/250 (48.40%), Query Frame = 1
Query: 1795 VIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQ----KCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPF-KLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSL 2529
            ++ K GDDLRQ+QL  QI+ ++ ++  +E   L + P  I+      G IE + N  ++  I   Y G  ++ Y K  +   N+   L  Q      FV+SCA Y ++ Y+L V DRH  N+L+  +GH  H D+G+IL   P       F P  KL  + ++  GG +S  ++ F+          R++   IL   E++ + +  +P             R+RF+L  +E    +   +++ +S+++L
Sbjct:  621 LMFKVGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPND-TLASILSKYHG--ILGYLKLHYPDENAT--LGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVTPDGHFFHADFGYILGQDPK-----PFPPLMKLPPQIIEAFGGAESSNYDKFRSYCFVAYSILRRNAGLILNLFELMKTSN--IPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVNAL 858          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Match: TEL1 (Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene [Source:SGD;Acc:S000000184])

HSP 1 Score: 66.6254 bits (161), Expect = 1.749e-11
Identity = 66/231 (28.57%), Postives = 104/231 (45.02%), Query Frame = 1
Query: 1810 GDDLRQEQLAYQILKMLQKVWLSERT----PLWVRPLNIVVISSDSGMIEPVLNAVSIHQI-KRLYSG---------------------KSLIEYFK--NQFGPTNSESFLN----------AQKCFVESCAAYSLVCYLLQVKDRHNGNILLD-NEGHMIHIDYGFILSASPGRNLGF-EFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEIL 2382
             DDLRQ+ +  Q+ + + KV  +++      L +R   +V +   +G+IE V N+ S+HQI  +L++                      + L  Y K  N+  P     F +          A+K + +  AA S+V Y+L + DRH  NILLD + G  IHID G  ++   G+ L   E  PF+LT + VD  G    D    F+    +     RK   K++  + IL
Sbjct: 2468 NDDLRQDAIMEQVFQQVNKVLQNDKVLRNLDLGIRTYKVVPLGPKAGIIEFVANSTSLHQILSKLHTNDKITFDQARKGMKAVQTKSNEERLKAYLKITNEIKPQLRNFFFDSFPDPLDWFEAKKTYTKGVAASSIVGYILGLGDRHLNNILLDCSTGEPIHIDLG--IAFDQGKLLPIPELVPFRLTRDIVDGFGVTGVDGL--FRRSCERVYAVLRKDYVKVMCVLNIL 2694          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Match: MEC1 (Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres [Source:SGD;Acc:S000000340])

HSP 1 Score: 54.299 bits (129), Expect = 8.345e-8
Identity = 56/209 (26.79%), Postives = 97/209 (46.41%), Query Frame = 1
Query: 1783 NLLSVIVKHGDDLRQE----QLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQI--------KRLYSGKSL------------IEYFKNQ---FGPTNSESFL----------NAQKCFVESCAAYSLVCYLLQVKDRHNGNILLD-NEGHMIHIDYGFILSASPGRNLGF-EFSPFKLTYEEVDIMGGVKSD 2292
            N+  ++ K  +D+RQ+    Q A  +  +L K   S +  L +   +++ +  D G++E V N V++  I        K  YS KSL            +E++  Q   F P   + FL          NA+  +  S A  ++V ++L + DRH  NILLD   G ++H+D+  +     G+ L   E  PF+LT   +D +G + ++
Sbjct: 2073 NIYGIMCKK-EDVRQDNQYMQFATTMDFLLSKDIASRKRSLGINIYSVLSLREDCGILEMVPNVVTLRSILSTKYESLKIKYSLKSLHDRWQHTAVDGKLEFYMEQVDKFPPILYQWFLENFPDPINWFNARNTYARSYAVMAMVGHILGLGDRHCENILLDIQTGKVLHVDFDCLFEK--GKRLPVPEIVPFRLTPNLLDALGIIGTE 2278          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Match: EDO42545 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S165])

HSP 1 Score: 171.785 bits (434), Expect = 1.260e-43
Identity = 117/365 (32.05%), Postives = 196/365 (53.70%), Query Frame = 1
Query: 1552 NIKEILDTDESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDK-------KVQR----------IKESSPY----GKIENWNLL--SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            NI  ++     G E       A GDIR   ++ ++L  + E + +  D +S    +   K       KV+R          +K+  P        EN N+   + I K GDD+RQ+ LA Q++ + + ++      ++++P  +V  S   G+IE V ++ S  Q+ R  +   L EY+   +G  ++ +F  A+  F++S AAYS+V +LLQ+KDRHNGN++LD EGH+IHID+GF+  +SPG NLGFE    K+++E V IMGG ++S  F +F  L ++  LA R + + +++ + ++  +   LPCF +G ET+    R RF   ++E +    +  ++ +S  +  TR+YD  Q   N I
Sbjct: 1326 NISAVIKPFPKGPERKEACLRALGDIRA--EKGVYLPSNPEAMVLEIDQTSGTPMQSAAKAPFLAKFKVKRMGIAELESINVKKDFPCDSDEANQENPNVYWQAAIFKVGDDVRQDMLALQVISLFKNIFEQVGLDVYLKPYRVVATSPGCGVIECVPDSKSRDQLGR-QTEVDLYEYYLKTYGDESTPAFQQARSNFIKSMAAYSIVSFLLQIKDRHNGNLMLDREGHIIHIDFGFMFESSPGGNLGFE-PDMKISHEMVMIMGGTMESPPFRWFMDLCVKSYLAVRPYQEALVSLVALM--LDTGLPCF-RG-ETL-KRLRARFSPAQSEKEAATYMSKVIRDSYLNYRTRVYDLIQLAQNQI 1681          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Match: EDO45547 (Phosphatidylinositol 3-kinase [Source:UniProtKB/TrEMBL;Acc:A7RSD9])

HSP 1 Score: 104.375 bits (259), Expect = 7.220e-23
Identity = 71/250 (28.40%), Postives = 129/250 (51.60%), Query Frame = 1
Query: 1795 VIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPV-LNAVSIHQIKRLYSGKSLIEYFKN----QFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSL 2529
            VI K+GDDLRQ+QL  Q+++++ K+ + E   L + P  ++  S+  G+++ +  NAV+    + L    S+  YF+     + GP            +V+SCA Y ++ YLL V DRH  N+LL   G++ HID+G+IL    GR+      P KL+ E V+ MGG   + ++ F+       L  R+H + IL    ++  +   +P  +   +      +D+F L  ++ +    + S+++ S+ ++
Sbjct:  624 VIFKNGDDLRQDQLILQMIRLMDKLLMKENLDLKLTPYKVLATSASHGLVQFIESNAVA----EVLDKDGSIQNYFRKHAPCETGPNGISP--EVMDTYVKSCAGYCVITYLLGVGDRHLDNLLLTKSGNLFHIDFGYIL----GRDPKPLPPPMKLSKEMVEGMGGANGECYQAFRKQCYTAFLHLRRHANLILNLFGLM--VDASVPDIALEPDKTVKKVQDKFRLDLSDEEAVQYLQSLIDESVSAM 861          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Match: EDO45977 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRA6])

HSP 1 Score: 93.2041 bits (230), Expect = 2.485e-19
Identity = 67/208 (32.21%), Postives = 104/208 (50.00%), Query Frame = 1
Query: 1759 PYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQ--------FGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILS-ASPGRNLGFEFSPFKLTYEEVDIM-GGVK-SDMFEYFKILILQGLLAARKH 2349
            P+G  EN N   VI K GDD+RQ+ L  Q++ ++ K+WL E   L +     +      GMIE V ++ ++ +I   +    L   FK++        F P   E +  A + F  SCA Y +  Y+L + DRHN NI++   GH+ HID+  IL  A    N   + +PF LT +   ++ GG + S  F++F  L  +     R+H
Sbjct:  746 PFG--ENVN---VIFKMGDDMRQDTLTMQLVGIMNKIWLQEGLDLKIITYQCMATGPGMGMIEIVSDSQTLREIHTEFG---LTGSFKDESINRWLLKFNPDEKE-YRRAVENFTASCAGYCVATYVLGICDRHNDNIMIHRSGHLFHIDFSKILGHAQMFGNFKRDRAPFVLTPDMAYVINGGYRPSPRFQHFIDLCSRAFNLIRRH 944          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Match: EDO42011 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J3])

HSP 1 Score: 91.2781 bits (225), Expect = 8.466e-19
Identity = 56/198 (28.28%), Postives = 100/198 (50.51%), Query Frame = 1
Query: 1789 LSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSG--------KSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGR-NLGFEFSPFKLTYEEVDIM--GGVKSDMFEYFKILILQGLLAARKH 2349
            + +I K GDDLRQ+ L  Q+++++ ++W  +   L + P   +      GMIE V N+ +I +I+    G          ++++  ++ G  + E+ + A   F+ SCA Y +  Y+L V DRH+ NI++   G + HID+G  L     +  +  E  PF LT   V ++  G   ++ F+ F+ +  Q  L  R+ 
Sbjct:  773 VKIIFKSGDDLRQDMLTLQLIRIMDRLWKKDGLDLEMIPYGCLSTGDHVGMIEVVTNSETIAKIQMKQMGAITGAFNKTCILDWLADRNG--SKEALVEATHRFMLSCAGYCVATYVLGVGDRHSDNIMVHENGQLFHIDFGHFLGNFKNKFGIRRERVPFVLTDHFVHVITRGNDNTEGFQEFRRICEQAFLVLRRR 968          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Match: EDO27738 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8E9])

HSP 1 Score: 71.2478 bits (173), Expect = 2.850e-13
Identity = 61/245 (24.90%), Postives = 107/245 (43.67%), Query Frame = 1
Query: 1795 VIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSL 2529
            VI K+GDDLRQ+QL  Q+++++ KV                                   +I   YS    I    + F P   +  +        S + Y ++ YLL V DRH  N+LL   G++ HID+G+IL    GR+      P KL+ E V+ MGG   + ++ F+       L  R+H + IL    ++  +   +P  +   +      +D+F L  ++ +    + S+++ S+ ++
Sbjct:  107 VIFKNGDDLRQDQLILQMIRLMDKV-----------------------------------RIFGPYSACGKIRSTFSDFAPPPPQEKILGTGLL--SVSGYCVITYLLGVGDRHLDNLLLTKSGNLFHIDFGYIL----GRDPKPLPPPMKLSKEMVEGMGGANGECYQAFRKQCYTAFLHLRRHANLILNLFGLM--VDASVPDIALEPDKTVKKVQDKFRLDLSDEEAVQYLQSLIDESVSAM 308          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Match: pi4kb (phosphatidylinositol 4-kinase beta [Source:NCBI gene;Acc:101165462])

HSP 1 Score: 473.781 bits (1218), Expect = 3.004e-154
Identity = 268/565 (47.43%), Postives = 339/565 (60.00%), Query Frame = 1
Query: 1000 LGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDL---VLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPG---------NIKEILDTDESGE---------------------------EFNGETFVAAGDIRRRLKENIFLQKHQETVKVN-DDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L  F P  + SLP    S S +  T   + S    S  LSS+L     +P +E           L PQ  FIK L+ I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+VVR+P   AV LNSK +APYLIYVEV+   N     +  + P +                      G         N  E    D+ GE                           E     F+AAGDIRRRL E   L +   T K + +DPS+  L EPW++KV+RI+E SPYG + NW LLSVIVK GDDLRQE LAYQ+L+ LQ +W  ER PLW++P  I+V+S+DSGMIEPV+NAVS+HQ+K+  S  SL++Y   + G   +E+FL AQ+ FV+SCA YSL+CYLLQVKDRHNGNILLD +GH+IHID+GFILS+SP RNLGFE S FKLT E VD+MGG   DMF YFK+L+LQGL+AARKHMD++L  +EI+   S  LPCF     +     ++RFH+  TE QLQ+L+D +V+ S+ SLTT+LYD FQY TNGI
Sbjct:  257 LTPFCPAPSGSLPGE-HSLSPAKRTHQRSKSDATVSISLSSNLKRTASNPKVE---SSPDEPVRLAPQREFIKSLIGIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAAFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFETSSVPVRIPENRIRSTRSVENLPDCGMTAEQRAGSFSIVPNYDNDDEAWSVDDIGELQVELPEGHTNSCDNISQFSVDSITSLESKEPIFIAAGDIRRRLSEQ--LAQAPTTFKRDPEDPSAVALKEPWEEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAYQVLQQLQSIWQQERVPLWIKPYKILVLSADSGMIEPVMNAVSLHQVKK-QSQLSLLDYLLQEHGAPTTEAFLTAQRNFVQSCAGYSLICYLLQVKDRHNGNILLDADGHIIHIDFGFILSSSP-RNLGFETSAFKLTSEFVDVMGGPDGDMFIYFKMLMLQGLIAARKHMDRVLQVVEIMQQGSH-LPCFHGS--STMRALKERFHMSLTEEQLQLLIDQLVDGSMRSLTTKLYDGFQYLTNGI 810          

HSP 2 Score: 91.6633 bits (226), Expect = 1.499e-18
Identity = 40/94 (42.55%), Postives = 61/94 (64.89%), Query Frame = 1
Query:  181 KQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPH-LSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            KQ  LLRLF+S +FD+ +AI YL    E G+QA++ N+ FS+    VDF+LPQL+ +++     + D  K Y  +RC++SI +S+   WL+ AY
Sbjct:  124 KQSWLLRLFESKLFDVSMAISYLHNSKEPGVQAYIGNRLFSFAHEEVDFYLPQLLNMYIHMDEDVGDAIKPYLVHRCRQSISFSLRCAWLLGAY 217          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Match: ENSORLT00000001557.2 (phosphatidylinositol 4-kinase beta-like [Source:NCBI gene;Acc:101161567])

HSP 1 Score: 470.315 bits (1209), Expect = 5.405e-153
Identity = 253/508 (49.80%), Postives = 330/508 (64.96%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP----------------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---EFNGET-----------------------FVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  F+K L  I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+V R+PP   V LNSK +APY+IY+EV+  ++     +  + P                 + ++  + +++          N  N  E   TD+ G+   E   +T                       F+AAGDIRRRL EN+    H  T    D  DPS+  L EPW++KV+RI+E+SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+V+SSDSGMIEPVLNAVS+HQ+K+  S  SL++YF  + G   +E+FL AQ+ FV+SCA YSL+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP +NLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K+L  +EI+   S  LPCF  G  T+    ++RFH+  TE QLQ+LV+ +V+ S+ S+TT+LYDSFQY TNGI
Sbjct:  305 LRPQREFVKSLFCIGKRLVTLPTKEQKTQRLISELSLLNHKLPARVWLPTAECQHHVCRLPPTQGVVLNSKDKAPYIIYIEVLECDSFETSPVPIRIPETRIHSSRSEESLDSGATANANSVITAEHRAGSFSTVPNYDNDDEAWATDDIGQLQVEMEAQTSSSDNISQFSVDSITSLESKEPMFIAAGDIRRRLSENL---AHPPTSFKWDPEDPSAVALKEPWEEKVRRIREASPYGHLANWKLLSVIVKCGDDLRQELLAYQVLKQLQSIWQQERVPLWIKPYKILVMSSDSGMIEPVLNAVSLHQVKK-QSQLSLLDYFLQEHGSFTTEAFLTAQRNFVQSCAGYSLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-KNLGFETSAFKLTSEFVDVMGGLDGDMFIYYKMLMLQGLIAARKHMEKVLQIVEIMQQGS-HLPCF-HGSSTI-RGLKERFHMSLTEEQLQVLVEQLVDGSMRSITTKLYDSFQYVTNGI 804          

HSP 2 Score: 90.1225 bits (222), Expect = 4.631e-18
Identity = 41/90 (45.56%), Postives = 61/90 (67.78%), Query Frame = 1
Query:  193 LLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAY-PHLSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            LLRLF+S +FD+ +AI YL K  E G+QA++ N+ FS+P S VDF+LPQL+ ++V     + D  + Y  +RC+ SI +S+   WL+ AY
Sbjct:  136 LLRLFESKLFDVSMAISYLYKSKEPGVQAYIGNRLFSFPDSEVDFYLPQLLNMYVHMDTEVGDSIRPYLIHRCRGSITFSLLSAWLLGAY 225          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Match: ENSORLT00000041935.1 (phosphatidylinositol 4-kinase beta-like [Source:NCBI gene;Acc:101161567])

HSP 1 Score: 470.7 bits (1210), Expect = 3.121e-151
Identity = 253/508 (49.80%), Postives = 330/508 (64.96%), Query Frame = 1
Query: 1183 LDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKP----------------IXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGE---EFNGET-----------------------FVAAGDIRRRLKENIFLQKHQETVKVND--DPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            L PQ  F+K L  I  RL   P+K QKT  L +EL+ LN  LPARVW+P     H+V R+PP   V LNSK +APY+IY+EV+  ++     +  + P                 + ++  + +++          N  N  E   TD+ G+   E   +T                       F+AAGDIRRRL EN+    H  T    D  DPS+  L EPW++KV+RI+E+SPYG + NW LLSVIVK GDDLRQE LAYQ+LK LQ +W  ER PLW++P  I+V+SSDSGMIEPVLNAVS+HQ+K+  S  SL++YF  + G   +E+FL AQ+ FV+SCA YSL+CYLLQVKDRHNGNILLD+EGH+IHID+GFILS+SP +NLGFE S FKLT E VD+MGG+  DMF Y+K+L+LQGL+AARKHM+K+L  +EI+   S  LPCF  G  T+    ++RFH+  TE QLQ+LV+ +V+ S+ S+TT+LYDSFQY TNGI
Sbjct:  467 LRPQREFVKSLFCIGKRLVTLPTKEQKTQRLISELSLLNHKLPARVWLPTAECQHHVCRLPPTQGVVLNSKDKAPYIIYIEVLECDSFETSPVPIRIPETRIHSSRSEESLDSGATANANSVITAEHRAGSFSTVPNYDNDDEAWATDDIGQLQVEMEAQTSSSDNISQFSVDSITSLESKEPMFIAAGDIRRRLSENL---AHPPTSFKWDPEDPSAVALKEPWEEKVRRIREASPYGHLANWKLLSVIVKCGDDLRQELLAYQVLKQLQSIWQQERVPLWIKPYKILVMSSDSGMIEPVLNAVSLHQVKK-QSQLSLLDYFLQEHGSFTTEAFLTAQRNFVQSCAGYSLICYLLQVKDRHNGNILLDSEGHIIHIDFGFILSSSP-KNLGFETSAFKLTSEFVDVMGGLDGDMFIYYKMLMLQGLIAARKHMEKVLQIVEIMQQGSH-LPCF-HGSSTI-RGLKERFHMSLTEEQLQVLVEQLVDGSMRSITTKLYDSFQYVTNGI 966          

HSP 2 Score: 90.8929 bits (224), Expect = 3.495e-18
Identity = 41/90 (45.56%), Postives = 61/90 (67.78%), Query Frame = 1
Query:  193 LLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAY-PHLSDVFKSYFTYRCKESIEYSISLVWLMDAY 459
            LLRLF+S +FD+ +AI YL K  E G+QA++ N+ FS+P S VDF+LPQL+ ++V     + D  + Y  +RC+ SI +S+   WL+ AY
Sbjct:  251 LLRLFESKLFDVSMAISYLYKSKEPGVQAYIGNRLFSFPDSEVDFYLPQLLNMYVHMDTEVGDSIRPYLIHRCRGSITFSLLSAWLLGAY 340          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Match: pik3c3 (phosphatidylinositol 3-kinase catalytic subunit type 3 [Source:NCBI gene;Acc:101175027])

HSP 1 Score: 108.997 bits (271), Expect = 7.191e-24
Identity = 76/273 (27.84%), Postives = 140/273 (51.28%), Query Frame = 1
Query: 1768 KIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFK----NQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQ----LQILVDSMVENSLHSLTTRLYDSFQYY 2562
            K E+  +  VI KHGDDLRQ+QL  QI+ ++ K+   E   L + P  ++  SS  G ++  + +V + ++  L +  ++  +F+    ++ GP    S       +V+SCA Y ++ Y+L V DRH  N+LL   G + HID+G+IL    GR+      P KL+ E V+ MGG++S+ ++ F+       L  R++ + IL    ++  +   +P  +   +      +D+F L  ++ +    +Q L+D  V     ++  +++   QY+
Sbjct:  612 KTEDGAMYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSSKHGFMQ-FVQSVPVAEV--LATEGNIQSFFRKHAPSEKGPYGISS--EVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYIL----GRDPKPLPPPMKLSKEMVEGMGGMQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLM--VDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQTLIDESVGALFAAVVEQIHKFAQYW 873          
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Match: pik3c3 (phosphatidylinositol 3-kinase catalytic subunit type 3 [Source:NCBI gene;Acc:101175027])

HSP 1 Score: 108.997 bits (271), Expect = 7.593e-24
Identity = 76/273 (27.84%), Postives = 140/273 (51.28%), Query Frame = 1
Query: 1768 KIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFK----NQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQ----LQILVDSMVENSLHSLTTRLYDSFQYY 2562
            K E+  +  VI KHGDDLRQ+QL  QI+ ++ K+   E   L + P  ++  SS  G ++  + +V + ++  L +  ++  +F+    ++ GP    S       +V+SCA Y ++ Y+L V DRH  N+LL   G + HID+G+IL    GR+      P KL+ E V+ MGG++S+ ++ F+       L  R++ + IL    ++  +   +P  +   +      +D+F L  ++ +    +Q L+D  V     ++  +++   QY+
Sbjct:  621 KTEDGAMYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSSKHGFMQ-FVQSVPVAEV--LATEGNIQSFFRKHAPSEKGPYGISS--EVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYIL----GRDPKPLPPPMKLSKEMVEGMGGMQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLM--VDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQTLIDESVGALFAAVVEQIHKFAQYW 882          
BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Match: SMESG000061430.1 (SMESG000061430.1)

HSP 1 Score: 1636.31 bits (4236), Expect = 0.000e+0
Identity = 816/819 (99.63%), Postives = 817/819 (99.76%), Query Frame = 1
Query:  121 MDVQEPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKPIXXXXXXXXXXXXXXXXXXXXXNPGNIKEILDTDESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGIQ 2577
            MDVQEPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLV+HPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKK ISTSSEKLDSSQSSLLQDVDCSNPGNIKEILDT ESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGIQ
Sbjct:    1 MDVQEPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFGIQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESIEYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQIITENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKSLQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRSKLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPVSTLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVMHPPIELKNKCSCASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARVWIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQKKSISTSSEKLDSSQSSLLQDVDCSNPGNIKEILDTYESGEEFNGETFVAAGDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGKIENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILATIEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGIQ 819          
BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Match: SMESG000041715.1 (SMESG000041715.1)

HSP 1 Score: 164.081 bits (414), Expect = 7.002e-41
Identity = 100/263 (38.02%), Postives = 152/263 (57.79%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEI-LDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++ + +   E    ++ P  +V  S   G+IE V ++ S  QI R  +   + +YF + FG   + S+  A++ FV S A+YS+VCYLLQ+KDRHNGNI+LD  GH+IHID+GF+  +SPG N+GFE    KLT E V +MGG + S  F++F+ L +Q  L  R + + I+A + + LDS    LPCF +G +T+    R RF    ++ +       ++           YD  QY+ N I
Sbjct: 1980 ACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPDSKSRDQIGRT-TDSGMYDYFLSLFGDETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMLDKHGHIIHIDFGFMFESSPGNNMGFE-PDIKLTKEMVMVMGGKIDSPAFQWFEQLSVQAYLVLRPYQEAIVALVSLMLDS---GLPCF-RG-QTI-KLLRQRFTPLMSDREAANHYLRVLRTCCDHWRANTYDFIQYFQNQI 2234          
BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Match: SMESG000041715.1 (SMESG000041715.1)

HSP 1 Score: 164.081 bits (414), Expect = 7.015e-41
Identity = 100/263 (38.02%), Postives = 152/263 (57.79%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEI-LDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++ + +   E    ++ P  +V  S   G+IE V ++ S  QI R  +   + +YF + FG   + S+  A++ FV S A+YS+VCYLLQ+KDRHNGNI+LD  GH+IHID+GF+  +SPG N+GFE    KLT E V +MGG + S  F++F+ L +Q  L  R + + I+A + + LDS    LPCF +G +T+    R RF    ++ +       ++           YD  QY+ N I
Sbjct: 1990 ACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPDSKSRDQIGRT-TDSGMYDYFLSLFGDETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMLDKHGHIIHIDFGFMFESSPGNNMGFE-PDIKLTKEMVMVMGGKIDSPAFQWFEQLSVQAYLVLRPYQEAIVALVSLMLDS---GLPCF-RG-QTI-KLLRQRFTPLMSDREAANHYLRVLRTCCDHWRANTYDFIQYFQNQI 2244          
BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Match: SMESG000041715.1 (SMESG000041715.1)

HSP 1 Score: 164.081 bits (414), Expect = 7.143e-41
Identity = 100/263 (38.02%), Postives = 152/263 (57.79%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEI-LDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++ + +   E    ++ P  +V  S   G+IE V ++ S  QI R  +   + +YF + FG   + S+  A++ FV S A+YS+VCYLLQ+KDRHNGNI+LD  GH+IHID+GF+  +SPG N+GFE    KLT E V +MGG + S  F++F+ L +Q  L  R + + I+A + + LDS    LPCF +G +T+    R RF    ++ +       ++           YD  QY+ N I
Sbjct: 1994 ACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPDSKSRDQIGRT-TDSGMYDYFLSLFGDETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMLDKHGHIIHIDFGFMFESSPGNNMGFE-PDIKLTKEMVMVMGGKIDSPAFQWFEQLSVQAYLVLRPYQEAIVALVSLMLDS---GLPCF-RG-QTI-KLLRQRFTPLMSDREAANHYLRVLRTCCDHWRANTYDFIQYFQNQI 2248          
BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Match: SMESG000041715.1 (SMESG000041715.1)

HSP 1 Score: 164.081 bits (414), Expect = 7.865e-41
Identity = 100/263 (38.02%), Postives = 152/263 (57.79%), Query Frame = 1
Query: 1792 SVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVVISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQKCFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRNLGFEFSPFKLTYEEVDIMGG-VKSDMFEYFKILILQGLLAARKHMDKILATIEI-LDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSLHSLTTRLYDSFQYYTNGI 2574
            + I K GDD+RQ+ LA Q++++ + +   E    ++ P  +V  S   G+IE V ++ S  QI R  +   + +YF + FG   + S+  A++ FV S A+YS+VCYLLQ+KDRHNGNI+LD  GH+IHID+GF+  +SPG N+GFE    KLT E V +MGG + S  F++F+ L +Q  L  R + + I+A + + LDS    LPCF +G +T+    R RF    ++ +       ++           YD  QY+ N I
Sbjct: 1996 ACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPDSKSRDQIGRT-TDSGMYDYFLSLFGDETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMLDKHGHIIHIDFGFMFESSPGNNMGFE-PDIKLTKEMVMVMGGKIDSPAFQWFEQLSVQAYLVLRPYQEAIVALVSLMLDS---GLPCF-RG-QTI-KLLRQRFTPLMSDREAANHYLRVLRTCCDHWRANTYDFIQYFQNQI 2250          
The following BLAST results are available for this feature:
BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PI4KB3.436e-15847.63phosphatidylinositol 4-kinase beta [Source:HGNC Sy... [more]
PI4KB3.436e-15847.63phosphatidylinositol 4-kinase beta [Source:HGNC Sy... [more]
PI4KB2.333e-15747.12phosphatidylinositol 4-kinase beta [Source:HGNC Sy... [more]
PI4KB2.409e-15747.12phosphatidylinositol 4-kinase beta [Source:HGNC Sy... [more]
PI4KB1.218e-15651.32phosphatidylinositol 4-kinase beta [Source:HGNC Sy... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pifk-12.004e-11443.35PhosphoInositide Four Kinase (PI-4 Kinase) [Sourc... [more]
Y75B8A.241.188e-4739.69pep chromosome:WBcel235:III:12251494:12273692:-1 g... [more]
Y75B8A.241.188e-4739.69pep chromosome:WBcel235:III:12251492:12273692:-1 g... [more]
piki-17.788e-1726.97Phosphatidylinositol 3-kinase piki-1 [Source:UniP... [more]
vps-341.212e-1625.47Phosphatidylinositol 3-kinase catalytic subunit ty... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fwd2.383e-10454.09gene:FBgn0004373 transcript:FBtr0072517[more]
fwd7.770e-10454.09gene:FBgn0004373 transcript:FBtr0072516[more]
fwd7.770e-10454.09gene:FBgn0004373 transcript:FBtr0072515[more]
fwd7.770e-10454.09gene:FBgn0004373 transcript:FBtr0332841[more]
fwd9.104e-10454.09gene:FBgn0004373 transcript:FBtr0474147[more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pi4kb9.362e-15651.20phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb9.362e-15651.20phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb9.362e-15651.20phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb1.351e-15551.20phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb1.351e-15551.20phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cobl2.540e-4037.05cordon-bleu WH2 repeat protein [Source:Xenbase;Acc... [more]
cobl2.699e-4037.05cordon-bleu WH2 repeat protein [Source:Xenbase;Acc... [more]
cobl2.743e-4037.05cordon-bleu WH2 repeat protein [Source:Xenbase;Acc... [more]
cobl2.878e-4037.05cordon-bleu WH2 repeat protein [Source:Xenbase;Acc... [more]
cobl2.883e-4037.05cordon-bleu WH2 repeat protein [Source:Xenbase;Acc... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pi4kb2.921e-15847.63phosphatidylinositol 4-kinase beta [Source:MGI Sym... [more]
Pi4kb1.226e-15747.12phosphatidylinositol 4-kinase beta [Source:MGI Sym... [more]
Pi4kb1.672e-15747.12phosphatidylinositol 4-kinase beta [Source:MGI Sym... [more]
Pi4kb8.826e-15751.32phosphatidylinositol 4-kinase beta [Source:MGI Sym... [more]
Pi4kb3.978e-8157.39phosphatidylinositol 4-kinase beta [Source:MGI Sym... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A4IID4|PI4KB_XENTR8.145e-15751.89Phosphatidylinositol 4-kinase beta OS=Xenopus trop... [more]
sp|B1MTG7|PI4KB_PLEMO8.397e-15747.12Phosphatidylinositol 4-kinase beta OS=Plecturocebu... [more]
sp|Q8BKC8|PI4KB_MOUSE8.579e-15747.12Phosphatidylinositol 4-kinase beta OS=Mus musculus... [more]
sp|B4UT09|PI4KB_OTOGA8.764e-15747.12Phosphatidylinositol 4-kinase beta OS=Otolemur gar... [more]
sp|Q6GN16|PI4KB_XENLA1.082e-15652.29Phosphatidylinositol 4-kinase beta OS=Xenopus laev... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1E1X2D80.000e+039.81Putative phosphatidylinositol 4-kinase beta OS=Amb... [more]
A0A2R5LNV90.000e+040.19Putative phosphatidylinositol 4-kinase (Fragment) ... [more]
A0A069DX321.258e-17939.78Putative phosphatidylinositol 4-kinase (Fragment) ... [more]
F1KUT53.087e-17138.82Phosphatidylinositol 4-kinase beta OS=Ascaris suum... [more]
A0A0A9YXT15.502e-17139.31Phosphatidylinositol 4-kinase beta (Fragment) OS=L... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pi4kb2.051e-15551.00phosphatidylinositol 4-kinase beta [Source:NCBI ge... [more]
pi4kb2.952e-15551.00phosphatidylinositol 4-kinase beta [Source:NCBI ge... [more]
pi4kab3.094e-4437.40phosphatidylinositol 4-kinase, catalytic, alpha b ... [more]
pi4kaa8.260e-4436.30phosphatidylinositol 4-kinase, catalytic, alpha a ... [more]
si:rp71-17i16.53.292e-2332.14phosphatidylinositol 4,5-bisphosphate 3-kinase cat... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pi4kb5.114e-15451.21phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb2.497e-12862.07phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
pi4kb1.318e-12450.82phosphatidylinositol 4-kinase, catalytic, beta [So... [more]
ENSPMAT00000008978.18.657e-4337.97pep scaffold:Pmarinus_7.0:GL477391:15964:91650:-1 ... [more]
pik3c2a1.974e-2228.74phosphatidylinositol-4-phosphate 3-kinase, catalyt... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
PIK12.298e-7643.14Phosphatidylinositol 4-kinase; catalyzes first ste... [more]
STT41.267e-4838.52Phosphatidylinositol-4-kinase; functions in the Pk... [more]
VPS342.105e-2027.60Phosphatidylinositol (PI) 3-kinase that synthesize... [more]
TEL11.749e-1128.57Protein kinase primarily involved in telomere leng... [more]
MEC18.345e-826.79Genome integrity checkpoint protein and PI kinase ... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO425451.260e-4332.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO455477.220e-2328.40Phosphatidylinositol 3-kinase [Source:UniProtKB/T... [more]
EDO459772.485e-1932.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO420118.466e-1928.28Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO277382.850e-1324.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pi4kb3.004e-15447.43phosphatidylinositol 4-kinase beta [Source:NCBI ge... [more]
ENSORLT00000001557.25.405e-15349.80phosphatidylinositol 4-kinase beta-like [Source:NC... [more]
ENSORLT00000041935.13.121e-15149.80phosphatidylinositol 4-kinase beta-like [Source:NC... [more]
pik3c37.191e-2427.84phosphatidylinositol 3-kinase catalytic subunit ty... [more]
pik3c37.593e-2427.84phosphatidylinositol 3-kinase catalytic subunit ty... [more]
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BLAST of phosphatidylinositol 4-kinase beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061430.10.000e+099.63SMESG000061430.1[more]
SMESG000041715.17.002e-4138.02SMESG000041715.1[more]
SMESG000041715.17.015e-4138.02SMESG000041715.1[more]
SMESG000041715.17.143e-4138.02SMESG000041715.1[more]
SMESG000041715.17.865e-4138.02SMESG000041715.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004928 ID=SMED30004928|Name=phosphatidylinositol 4-kinase beta|organism=Schmidtea mediterranea sexual|type=transcript|length=2653bp
TTTCTTATTAAATTTACATACATTTTTTTAATTTTGTTAATAAACATATT
GTTAAACTATTTTTTTTTCAAGAAAATCTATTTTTATTAGAATTTTAGTT
AAATGTGATTTTAAGGTAAAATGGATGTACAAGAACCGGTTAATAATTTT
GAATCAATGTGTACTAATAATACTTGTGAAAAACAAGAAGGTTTGCTAAG
ATTATTCCAATCGTCGATCTTTGATATGGATTTAGCGATTCAATATCTCT
CAAAATATACTGAATTTGGTATTCAGGCATTTCTAGTAAATAAATTTTTT
TCATATCCGAAATCTTCCGTCGACTTTTTTCTGCCTCAGCTAGTCTGCTT
ATTTGTCGCTTATCCTCATTTAAGTGATGTTTTCAAATCATATTTTACAT
ATAGGTGCAAAGAATCGATCGAATATAGCATTTCTTTAGTTTGGTTAATG
GATGCATATGTCAATGGCTATATTGCAATAAACAATGACATTAAGAGAAG
TTCATTATCTTTAGACGCAAATTTCATTGCAAATTTGGAACATGTAAAAA
AATTTAGAAATCAAATTATCACTGAAAATTCAAGCTTTATTCATTTAATT
TGTGAAAACCGATTAACTATTAAAGATTCTACCGATGATATTTATAGAAA
TTATAAAAATCATAAAAGATCAACATCCGATCTCTCAGGATTGAGTCGTG
TAAATTCGATAAATAAATCTCTTCAAATGAGTTTCTATAACAATGATCTC
ATGACTGAATCATATCGAAACGGTCAACTGAATTATTGTAATGGAGGGGA
AAACAATACAGTCGATGACATCAAGTCTCTGGATTCCGATGAAAACAATT
TTTATAATATCAATAGATCAAAGTTGGCGAATCACGATTGCCAATCTGTG
AATAGTTTACCAGATTTATTATCAGGCCACGCATTTGACAATGTGTGCAA
TGCGTTTAATCGGAACTCATTCCCTAATATTTATGGTAGTCGCAAAGAAT
TAGGGAACTTTAGACCAGTCAGTACATTGAGCTTACCTGCTAATTGTAAA
TCCCACTCATCATCATATTCGACTTTCAATTGCAACAATTCAACCAAATT
ACACTCAGAACCACTTTCTTCTGATTTGGTATTGCATCCACCAATCGAAT
TAAAGAATAAATGCAGCTGTGCCAGCTATTTCCTGGACCCACAGTTGAGT
TTTATTAAAATCCTCGTATCTATCTCAATGAGACTGACTCGGTACCCATC
GAAATATCAGAAAACCAATCATTTGCGAGCTGAGTTGGCTCATCTAAATC
TTAATCTTCCAGCCAGAGTGTGGATACCGATTTATGGCGCCAGTCATTAC
GTCGTTAGGATACCTCCAAATGCTGCTGTTTGTTTGAATTCTAAAATGAG
AGCGCCATATTTAATTTATGTGGAAGTTGTAGCGGCTAATAATTTATTCT
ACGATGAATTAATTCAAAAGAAACCCATTTCGACGTCAAGTGAAAAATTG
GATTCGAGCCAGTCGTCACTGTTGCAAGATGTCGATTGTTCGAATCCAGG
AAACATAAAAGAAATATTAGACACAGATGAATCCGGAGAAGAATTCAATG
GGGAGACATTTGTAGCTGCAGGAGACATTCGTCGGCGACTTAAAGAGAAT
ATATTCTTACAAAAACATCAAGAAACTGTGAAAGTAAATGACGATCCAAG
TTCATCAGTACTATTTGAGCCTTGGGATAAAAAAGTTCAACGAATCAAAG
AATCTTCTCCTTATGGTAAAATAGAAAATTGGAATTTGTTATCTGTAATC
GTTAAGCACGGTGATGATTTAAGACAAGAACAATTGGCTTATCAAATTTT
AAAAATGCTCCAGAAAGTTTGGCTCTCCGAAAGAACTCCGCTTTGGGTAA
GGCCGTTAAATATCGTTGTGATCTCATCTGATAGTGGCATGATCGAACCA
GTTCTCAATGCGGTGTCTATACACCAAATTAAAAGACTTTATTCTGGGAA
ATCTTTGATCGAATATTTTAAAAATCAATTTGGTCCTACAAATTCCGAAA
GTTTTCTCAATGCCCAAAAATGTTTTGTTGAAAGTTGTGCCGCATATTCT
CTGGTGTGTTACTTGTTGCAAGTAAAGGACAGGCATAATGGAAATATCCT
TCTCGATAATGAAGGCCACATGATCCATATAGATTATGGATTTATTTTAT
CTGCGTCACCAGGAAGAAATCTTGGATTTGAATTTTCACCATTCAAACTG
ACTTACGAAGAAGTCGATATAATGGGTGGTGTAAAGAGTGACATGTTCGA
ATATTTTAAGATACTAATTCTTCAAGGATTATTGGCGGCTCGAAAGCACA
TGGATAAGATACTTGCAACTATTGAGATATTAGATTCCATTTCTTTCAAG
CTGCCTTGTTTCAGTAAGGGATTCGAAACAGTTTATAACAATTTCCGGGA
CAGATTTCATCTGTGCAAAACAGAAGCCCAATTACAGATTCTAGTTGACA
GTATGGTGGAAAATAGTCTTCATTCGCTTACAACGCGACTGTATGACAGT
TTCCAATACTATACTAATGGCATCCAATGAATACTTTGCAATCAAATCTG
CTTGTAAATGTCTTTCAATAAAATATGCAACAAGACTTTGTTTAAATTTC
ATA
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protein sequence of SMED30004928-orf-1

>SMED30004928-orf-1 ID=SMED30004928-orf-1|Name=SMED30004928-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=820bp
MDVQEPVNNFESMCTNNTCEKQEGLLRLFQSSIFDMDLAIQYLSKYTEFG
IQAFLVNKFFSYPKSSVDFFLPQLVCLFVAYPHLSDVFKSYFTYRCKESI
EYSISLVWLMDAYVNGYIAINNDIKRSSLSLDANFIANLEHVKKFRNQII
TENSSFIHLICENRLTIKDSTDDIYRNYKNHKRSTSDLSGLSRVNSINKS
LQMSFYNNDLMTESYRNGQLNYCNGGENNTVDDIKSLDSDENNFYNINRS
KLANHDCQSVNSLPDLLSGHAFDNVCNAFNRNSFPNIYGSRKELGNFRPV
STLSLPANCKSHSSSYSTFNCNNSTKLHSEPLSSDLVLHPPIELKNKCSC
ASYFLDPQLSFIKILVSISMRLTRYPSKYQKTNHLRAELAHLNLNLPARV
WIPIYGASHYVVRIPPNAAVCLNSKMRAPYLIYVEVVAANNLFYDELIQK
KPISTSSEKLDSSQSSLLQDVDCSNPGNIKEILDTDESGEEFNGETFVAA
GDIRRRLKENIFLQKHQETVKVNDDPSSSVLFEPWDKKVQRIKESSPYGK
IENWNLLSVIVKHGDDLRQEQLAYQILKMLQKVWLSERTPLWVRPLNIVV
ISSDSGMIEPVLNAVSIHQIKRLYSGKSLIEYFKNQFGPTNSESFLNAQK
CFVESCAAYSLVCYLLQVKDRHNGNILLDNEGHMIHIDYGFILSASPGRN
LGFEFSPFKLTYEEVDIMGGVKSDMFEYFKILILQGLLAARKHMDKILAT
IEILDSISFKLPCFSKGFETVYNNFRDRFHLCKTEAQLQILVDSMVENSL
HSLTTRLYDSFQYYTNGIQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR036940PI3/4_kinase_cat_sf
IPR015433PI_Kinase
IPR000403PI3/4_kinase_cat_dom
IPR018936PI3/4_kinase_CS
IPR001263PI3K_accessory_dom
IPR011009Kinase-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0016301kinase activity
GO:0000166nucleotide binding
GO:00044301-phosphatidylinositol 4-kinase activity
GO:0005524ATP binding
GO:0016740transferase activity
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005739mitochondrion
GO:0005741mitochondrial outer membrane
GO:0005783endoplasmic reticulum
GO:0012505endomembrane system
GO:0016020membrane
GO:0030867rough endoplasmic reticulum membrane
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation
GO:0046854phosphatidylinositol phosphorylation
GO:0048015phosphatidylinositol-mediated signaling
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainSMARTSM00146pi3k_hr1_6coord: 558..817
e-value: 1.2E-86
score: 303.9
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPFAMPF00454PI3_PI4_kinasecoord: 558..753
e-value: 3.2E-28
score: 99.2
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPROSITEPS50290PI3_4_KINASE_3coord: 557..818
score: 53.284
NoneNo IPR availableGENE3DG3DSA:3.30.1010.10coord: 536..611
e-value: 1.4E-14
score: 56.1
NoneNo IPR availablePANTHERPTHR10048:SF22PHOSPHATIDYLINOSITOL 4-KINASE BETAcoord: 478..819
NoneNo IPR availablePANTHERPTHR10048:SF22PHOSPHATIDYLINOSITOL 4-KINASE BETAcoord: 23..467
NoneNo IPR availableCDDcd05168PI4Kc_III_betacoord: 527..818
e-value: 1.98524E-165
score: 478.9
IPR036940Phosphatidylinositol 3-/4-kinase, catalytic domain superfamilyGENE3DG3DSA:1.10.1070.11coord: 616..819
e-value: 7.8E-55
score: 187.6
IPR015433Phosphatidylinositol kinasePANTHERPTHR10048PHOSPHATIDYLINOSITOL KINASEcoord: 478..819
IPR015433Phosphatidylinositol kinasePANTHERPTHR10048PHOSPHATIDYLINOSITOL KINASEcoord: 23..467
IPR018936Phosphatidylinositol 3/4-kinase, conserved sitePROSITEPS00915PI3_4_KINASE_1coord: 561..575
IPR018936Phosphatidylinositol 3/4-kinase, conserved sitePROSITEPS00916PI3_4_KINASE_2coord: 655..675
IPR001263Phosphoinositide 3-kinase, accessory (PIK) domainPROSITEPS51545PIK_HELICALcoord: 1..137
score: 8.874
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 357..813