SMED30004893

Overview
NameSMED30004893
Smed IDSMED30004893
Length (bp)2285
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004893 (SMED30004893) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004893 (SMED30004893) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004893 (SMED30004893) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004893 (SMED30004893) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30004893

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30004893SMESG000071513.1 SmedASXL_011229SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30004893SMESG000071513.1 dd_Smed_v4_7180_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004893SMESG000036452.1 SMESG000006455.1 dd_Smed_v4_7629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004893SMESG000036452.1 SMESG000006455.1 dd_Smed_v4_7629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30004893 vs. Planmine SMEST
Match: SMESG000071513.1 (SMESG000071513.1)

HSP 1 Score: 1319.68 bits (3414), Expect = 0.000e+0
Identity = 660/661 (99.85%), Postives = 661/661 (100.00%), Query Frame = 3 
Query:   75 MNTNLIFITNIPKSQRPILLLKELNNKWSKMVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIDEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCXXXXXXXXXXXXXVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 2057
            MNTNLIFITNIPKSQRPILLLKELNNKWSKMVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEI+EGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD
Sbjct:    1 MNTNLIFITNIPKSQRPILLLKELNNKWSKMVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIEEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 661
[Close]
BLAST of SMED30004893 vs. Planmine SMEST
Match: SMESG000071513.1 (SMESG000071513.1)

HSP 1 Score: 1260.74 bits (3261), Expect = 0.000e+0
Identity = 630/631 (99.84%), Postives = 631/631 (100.00%), Query Frame = 3 
Query:  165 MVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIDEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCXXXXXXXXXXXXXVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 2057
            MVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEI+EGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD
Sbjct:    1 MVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIEEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 631
[Close]
BLAST of SMED30004893 vs. Planmine SMEST
Match: SMESG000071513.1 (SMESG000071513.1)

HSP 1 Score: 1042.72 bits (2695), Expect = 0.000e+0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 3 
Query:  489 WIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCXXXXXXXXXXXXXVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 2057
            WIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD
Sbjct:   41 WIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPKKSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVWMKEPPEKRFKD 563

HSP 2 Score: 82.4185 bits (202), Expect = 4.885e-16
Identity = 39/41 (95.12%), Postives = 40/41 (97.56%), Query Frame = 1 
Query:  229 MLIYLIKELKNYEEKKLMKALHYKCIMSGFLHQTFIYQLIY 351
            MLIYLIKELKNYEEKKL KALHYKCIMSGFLHQTFIYQLI+
Sbjct:    1 MLIYLIKELKNYEEKKLRKALHYKCIMSGFLHQTFIYQLIW 41
[Close]
BLAST of SMED30004893 vs. Planmine SMEST
Match: SMESG000024144.1 (SMESG000024144.1)

HSP 1 Score: 713.761 bits (1841), Expect = 0.000e+0
Identity = 356/643 (55.37%), Postives = 486/643 (75.58%), Query Frame = 3 
Query:   75 MNTNLIFITNIPKSQRPIL---LLKELNNKWSKMVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIDEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCXXXXXXXXXXXXXVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDRKPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRS 1994
            MN+N IFI++IPK Q       L  +L+NKWS+ +YID+P +E VD F+YN T ++  FDKG+E+L+RKE +    LQVYY+WFP PNV +++ IP+G+ MLPY++VD +PK +EV++VIG+LPFKAND KEWDI  K+DK +V+RLP   D+ TRNY L YLRNK++ KG+ PRVTE+F+S+E  LS+IM PK   SL+  +SYK NLIF YDLP R++ H IVDAL+D+DYRHCKAYL+D+ +Q RLGFIS SKS P  KF +L+ KLKT+ I+  S GK+KLNISEVWLQPVNC+PP+ KKS++  KVEF+L NYIYLHN+S S+Q  DLT KSPV FRV++Y  QDS  QIGF  + D+ E KV ++I YLRSL+F+ LP+LVDE+WIKE DLPEI DR+ VH  RK   S E        + T+E ++II+E+   ++F IK A++PP+ SNL KS++I L NLP + N S    D+N+   GLCLM++  EK+ FLYHRQVS++KW++ L+KI  +++N RRI+ KEIYLM DE+I+LIQ+KY +IYLS+Y+FVYN+G + +  ++E D H+ I++H  M  D+  RC+F+QLR+D++P +IIEVL +IRTL + GK+++ADL+WLAN+ + +  RS
Sbjct:    1 MNSNFIFISSIPKCQISNFSKDLDSKLDNKWSRTLYIDDPCNESVDVFIYNPTSSISHFDKGVERLKRKE-ENCVQLQVYYIWFPAPNVNMASLIPKGTNMLPYLIVDCIPKYVEVHDVIGDLPFKANDFKEWDISYKEDKCLVLRLPKCPDEITRNYALCYLRNKKLPKGVLPRVTEVFISKEPRLSKIMFPKIWYSLNETNSYKSNLIFIYDLPPRISSHDIVDALRDIDYRHCKAYLVDVVDQLRLGFISTSKSMPADKFEDLVNKLKTISIDAGSLGKLKLNISEVWLQPVNCEPPRVKKSDADRKVEFVLTNYIYLHNLSQSIQPLDLTLKSPVNFRVNLYQCQDSKRQIGFVPLIDMSERKVNKIIDYLRSLVFEGLPVLVDEIWIKEYDLPEIEDRRLVHCPRKSSRSFE-----NFQNRTIEALDIIEEVNLNRQFGIKCADEPPSFSNLHKSNIILLRNLPLDFNGSTDSLDLNI-TSGLCLMESVNEKIGFLYHRQVSDVKWMDFLEKISFSNVNGRRIIVKEIYLMYDESIDLIQTKYQAIYLSNYIFVYNIGMNGDLKLDELDKHSSIVKHVSMNADNGARCSFIQLREDISPFEIIEVLQFIRTLKVCGKVLFADLVWLANVNIDESSRS 636
[Close]
BLAST of SMED30004893 vs. Planmine SMEST
Match: SMESG000052914.1 (SMESG000052914.1)

HSP 1 Score: 514.998 bits (1325), Expect = 9.625e-174
Identity = 284/678 (41.89%), Postives = 430/678 (63.42%), Query Frame = 3 
Query:   75 MNTNLIFITNIPKSQRPILLLKELNNKWSKMVYIDNPYSELVDAFVYNLTPNVDLFDKGIEKLRRKEIDEGTPLQVYYVWFPPPNVYISTNIPEGSIMLPYILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDDSTRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIHSYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFISASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCX-XXXXXXXXXXXXVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNTQIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPE-ISDRKPVHISRK-IDTSNELIS-----LKTINSVTVE--TVEIID-----EICAGKEFIIKLAEDPPTSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLYHRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIYLSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDLTPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDP---RNVWMKE--PPEKR 2048
            MNTNLI +TNIPK  R   L+KEL NKW+++++IDNPY++L +AF+YN T  +  F+ G+ +LR K I+   PL+VY++WFPPPNVY++  IPE + MLP + VD+LPKE +  EVIG+LPF++NDVK WDIPQK+DKW+V+RLP+  D++ R+YVL Y+R +R+  G+FPRV E+FVSE+  LS+ MVPK+L+ L I  S+K NL+F YDLPT+LT   I DA K L  +  KA+ ID+ +  R+GF+   KSFPD KF    + LK  EI V S   V LN++EV+LQP     P   KK +    +E++L NY+Y+HN+++ +  + L   S + F+++++   ++ TQ+GF  ++ + +  + +LI +LRSL+F+   ++VD +++K  D+P+  S+R+ VH+ R+ +D  + + S        I  V +E   VE+ID     +I   KE  ++++ DPP   NLMKS  IF+  LP  +  S     + + C+ L +M     K+AFLY+   +  KW+  L+K+ +T I +  +   E+YL SDE  E++      I  S+YVFVYN+G  S+    E +T+  I+R   MT +S + CAFV+L   LT  + IE LNYIR +++ GK + ADL+WL        VR+ +  DP   R+ W+K+  PP K+
Sbjct:    1 MNTNLILVTNIPKHIRHFDLIKELENKWARLIFIDNPYADLQEAFIYNPTSLLTHFEMGVSRLRSKVINGIRPLKVYHLWFPPPNVYLTNLIPENATMLPVLFVDNLPKECDSYEVIGDLPFQSNDVKHWDIPQKEDKWMVLRLPVNQDEAIRDYVLKYIRTRRLSNGLFPRVLEVFVSEDIRLSKNMVPKTLQILPITASFKSNLLFLYDLPTKLTVKGISDAAKALGVKGSKAFFIDVVDSLRVGFLFVPKSFPDSKFEFFQEALKEKEIVVDSNQTVMLNLNEVYLQPKGALPPKVHKKRDDDRTIEYVLTNYVYVHNVAAGIDHNQLAKGSTINFKIYLFQIFENTTQVGFVCLRGLSDVAISQLIDHLRSLVFEGFQVMVDNIYLKCYDMPDNNSERRTVHVPREPLDNVDNITSHVDNTFVDITEVNLEESEVEVIDTDEENDIEMLKECSVEISSDPPPEYNLMKSDFIFVNYLPNTVEYSSFSL-LPLECDSLTIMSCDEHKIAFLYYPDTTTDKWMGALNKLVTTKIKETNVEVSEVYLQSDENEEMLDKGLDMISPSNYVFVYNIGDCSDTRKLEGNTN--IVRFSTMTQESGS-CAFVELASGLTALEKIETLNYIRDISLRGKNLCADLIWLG------TVRNKTKSDPNALRSQWLKKSGPPSKK 668
The following BLAST results are available for this feature:
BLAST of SMED30004893 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004893 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000071513.10.000e+099.85SMESG000071513.1[more]
SMESG000071513.10.000e+099.84SMESG000071513.1[more]
SMESG000071513.14.885e-16100.00SMESG000071513.1[more]
SMESG000024144.10.000e+055.37SMESG000024144.1[more]
SMESG000052914.19.625e-17441.89SMESG000052914.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004893 ID=SMED30004893|Name=SMED30004893|organism=Schmidtea mediterranea sexual|type=transcript|length=2285bp
TTTTGAATTTTTTTTATGTCAGTTAGTTTTTCTTTACATATTTGGAATAT
TTATTAAATTATTTATTGAAAATCATGAATACTAATTTAATTTTTATAAC
TAACATTCCTAAATCACAAAGGCCAATTTTATTATTAAAAGAGTTGAACA
ATAAATGGTCAAAAATGGTATATATCGACAATCCGTATTCAGAATTAGTT
GATGCATTTGTTTACAACCTAACTCCAAATGTTGATCTATTTGATAAAGG
AATTGAAAAATTACGAAGAAAAGAAATTGATGAAGGCACTCCATTACAAG
TGTATTATGTCTGGTTTCCTCCACCAAACGTTTATATATCAACTAATATA
CCAGAAGGATCAATTATGCTACCTTACATATTGGTGGATAGTTTACCTAA
AGAGATAGAGGTGAATGAAGTTATTGGAAATTTGCCGTTCAAGGCAAATG
ATGTTAAAGAGTGGGATATTCCACAGAAAGATGACAAATGGATTGTTATA
AGATTGCCAATAACGAACGATGACAGCACCAGAAATTATGTTTTAAATTA
TTTAAGAAATAAAAGAATAGGGAAAGGAATATTTCCTCGAGTTACTGAAA
TGTTCGTATCAGAAGAATCTTGCTTGTCGAGAATTATGGTTCCGAAATCT
TTAAGATCTTTGTCTATTATTCACTCATATAAATGTAATCTTATTTTCAC
GTACGACTTACCAACCAGGCTTACAGGTCATCATATAGTCGACGCTCTAA
AGGATCTCGATTACCGGCATTGCAAAGCGTATTTGATCGATATTACTGAA
CAACAAAGACTGGGATTTATTTCTGCTTCTAAATCATTTCCTGATTACAA
ATTTAATGAATTAATTCAAAAGTTAAAAACTCTTGAAATCGAAGTGACGT
CGTTTGGTAAAGTAAAATTAAATATAAGCGAAGTTTGGTTACAGCCCGTT
AACTGCAAACCGCCAAAACCTAAAAAATCCGAATCTACGAGCAAAGTTGA
ATTTATTTTGATCAACTACATCTACCTACATAACATCAGCTCTTCCGTTC
AATCTTCCGATTTGACAAATAAGTCCCCTGTACCATTTAGAGTACACATC
TATCACAGCCAGGATTCTAATACCCAAATCGGGTTTGCATCAGTTCAAGA
TATTCATGAATCAAAAGTTGGCAGACTGATAACTTATTTAAGATCTTTGA
TATTCGATTGTTTACCGTTGCTAGTGGATGAAATGTGGATTAAAGAATGT
GATTTGCCAGAAATCTCCGATAGGAAACCAGTTCATATTTCGAGGAAAAT
AGATACCAGTAACGAACTCATCTCTTTAAAAACTATCAATTCGGTTACAG
TGGAAACGGTTGAAATTATAGATGAGATTTGTGCTGGGAAAGAATTCATT
ATAAAATTAGCAGAAGATCCCCCAACATCATCCAATTTGATGAAATCACA
CATGATATTTTTAATGAACCTTCCACCAAATTTAAACAAGAGCAGAATAA
TTTTCGACATGAATGTTCCATGTGAAGGATTATGTTTAATGCAGACGGCG
ACAGAAAAAGTGGCATTTCTCTATCACAGACAAGTATCAGAATTGAAATG
GCTAGAAGTATTGGATAAAATATGTTCCACGAGTATCAATGATAGGCGCA
TTGTTGCTAAAGAAATTTATTTGATGTCTGACGAAACGATTGAATTAATC
CAATCAAAATATCCGTCAATATATCTGAGTAGCTATGTTTTTGTGTACAA
CCTTGGATCTGACAGTGAGCCGAATATGGAGGAATTCGACACACATGCGT
ACATTATTAGGCATTGTTTTATGACTACCGATTCCAATACCAGATGTGCT
TTTGTCCAATTGCGAGACGATTTGACTCCTCCACAGATTATTGAGGTTTT
GAATTATATTCGCACTCTCAATATCGGTGGCAAAATCGTCTGGGCAGATC
TTATGTGGCTAGCAAATATCACTCTGATGGATGTTGTCCGATCGGCCAGT
TTCGGTGATCCTCGAAATGTTTGGATGAAAGAACCTCCAGAAAAACGATT
CAAAGATTAATATTTTAATTCCACGCATAAAAATATTTATTTTTATTTCT
TTGTTATTATGATATTGTTTACTTTATATTTATAGTTGCAACTCTTCAAG
TTGATGTTAATTTTAAATTCGTTTTATTATTATTATTAATAATAATAATA
TTTTCTAATTCATTGAAATTTATCACCATGTAAGTCTGTGCAACGGTATC
GGTGGTTATGATTAAATTTCTTTGCTTTGACCATC
back to top

protein sequence of SMED30004893-orf-1

>SMED30004893-orf-1 ID=SMED30004893-orf-1|Name=SMED30004893-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=662bp
MNTNLIFITNIPKSQRPILLLKELNNKWSKMVYIDNPYSELVDAFVYNLT
PNVDLFDKGIEKLRRKEIDEGTPLQVYYVWFPPPNVYISTNIPEGSIMLP
YILVDSLPKEIEVNEVIGNLPFKANDVKEWDIPQKDDKWIVIRLPITNDD
STRNYVLNYLRNKRIGKGIFPRVTEMFVSEESCLSRIMVPKSLRSLSIIH
SYKCNLIFTYDLPTRLTGHHIVDALKDLDYRHCKAYLIDITEQQRLGFIS
ASKSFPDYKFNELIQKLKTLEIEVTSFGKVKLNISEVWLQPVNCKPPKPK
KSESTSKVEFILINYIYLHNISSSVQSSDLTNKSPVPFRVHIYHSQDSNT
QIGFASVQDIHESKVGRLITYLRSLIFDCLPLLVDEMWIKECDLPEISDR
KPVHISRKIDTSNELISLKTINSVTVETVEIIDEICAGKEFIIKLAEDPP
TSSNLMKSHMIFLMNLPPNLNKSRIIFDMNVPCEGLCLMQTATEKVAFLY
HRQVSELKWLEVLDKICSTSINDRRIVAKEIYLMSDETIELIQSKYPSIY
LSSYVFVYNLGSDSEPNMEEFDTHAYIIRHCFMTTDSNTRCAFVQLRDDL
TPPQIIEVLNYIRTLNIGGKIVWADLMWLANITLMDVVRSASFGDPRNVW
MKEPPEKRFKD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR001024PLAT/LH2_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding