SMED30004833
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004833 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SMED30004833 vs. TrEMBL
Match: D2CG37 (Uncharacterized protein OS=Tribolium castaneum OX=7070 GN=GLEAN_10694 PE=4 SV=1) HSP 1 Score: 73.559 bits (179), Expect = 2.968e-14 Identity = 38/89 (42.70%), Postives = 48/89 (53.93%), Query Frame = -1 Query: 250 MLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW 513 +L KLK G+ I+ C G+ Y+NA M G H G+ R I K V C NH N A VH A VN++TFFG +E+ F S S W Sbjct: 2 ILQKLKKDGIDIQNCRGQAYDNAAVMAGQHTGVQKRIKEINPKVEFVACTNHSLNLAGVHAASVAVNSVTFFGTMERLFLFFSSSTHRW 90 HSP 2 Score: 35.4242 bits (80), Expect = 2.968e-14 Identity = 26/93 (27.96%), Postives = 43/93 (46.24%), Query Frame = -3 Query: 2 NKSVKRVCETH*SSKHDAVASNTEGLVNLY-----------NSCVRAILKLQKLTLEIFILICFISSFQTYLMKEIKETQNTLQPKDLGLYKC 247 + VKR+ ET S++ DAV+ + + N R+ + L+ F +CF+ F ++KEI ++Q LQ K L L +C Sbjct: 98 GQDVKRLIETRWSARGDAVSVVRKAYSKILTVLEDLTGEKENGTTRSDAGVLLAALQSFSFLCFL-GFWDSVLKEINDSQAYLQTKKLNLQQC 189
BLAST of SMED30004833 vs. TrEMBL
Match: A0A3L8D616 (Dimer_Tnp_hAT domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_011674 PE=4 SV=1) HSP 1 Score: 72.4034 bits (176), Expect = 1.334e-13 Identity = 35/92 (38.04%), Postives = 51/92 (55.43%), Query Frame = -1 Query: 250 TEQMLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW 522 T +L K+ GL I+ C G+ Y+NA M G+H + +R +I +A V C NH N A +H A + V ++TFFG +E+ F S S W Sbjct: 18 TAMILTKISKDGLDIQNCRGQAYDNAAVMAGVHTRVQVRIKSINPRAQFVACTNHSLNLAGIHAASEAVQSVTFFGTVEQVFVFFSSSTHRW 109 HSP 2 Score: 33.8834 bits (76), Expect = 1.334e-13 Identity = 25/100 (25.00%), Postives = 47/100 (47.00%), Query Frame = -3 Query: 2 LVNKSVKRVCETH*SSKHDAVASNTEGLVNLYNSCVRAILKLQKLT----------------LEIFILICFISSFQTYLMKEIKETQNTLQPKDLGLYKC 253 + + VKR+ ET S++H+AV E N ++ + + KL T L+ +C++ + + ++KE+ + QN LQ K L + +C Sbjct: 115 VTGQGVKRLIETRWSARHEAV----EAFKNHFSDILNVLEKLASDTNENIVTRSEAGLLLKALQSLSFLCYLGLW-SQVLKEVNDAQNYLQTKGLNIEQC 209
BLAST of SMED30004833 vs. TrEMBL
Match: A0A026WGR2 (Zinc finger MYM-type protein OS=Ooceraea biroi OX=2015173 GN=X777_05348 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 1.900e-13 Identity = 34/89 (38.20%), Postives = 50/89 (56.18%), Query Frame = -1 Query: 250 MLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW 513 +L K+ GL I+ C G+ Y+NA M G+H + +R +I +A V C NH N A +H A + V ++TFFG +E+ F S S W Sbjct: 2 ILTKISKDGLDIQNCRGQAYDNAAVMAGVHTRVQVRIKSINPRAQFVACTNHSLNLAGIHAASEAVQSVTFFGTVEQVFVFFSSSTHRW 90 HSP 2 Score: 34.2686 bits (77), Expect = 1.900e-13 Identity = 25/100 (25.00%), Postives = 47/100 (47.00%), Query Frame = -3 Query: 2 LVNKSVKRVCETH*SSKHDAVASNTEGLVNLYNSCVRAILKLQKLT----------------LEIFILICFISSFQTYLMKEIKETQNTLQPKDLGLYKC 253 + + VKR+ ET S++H+AV E N ++ + + KL T L+ +C++ + + ++KE+ + QN LQ K L + +C Sbjct: 96 VTGQGVKRLIETRWSARHEAV----EAFKNHFSDILNVLEKLASDTNENIVTRSEAGLLLKALQSLSFLCYLGLW-SQVLKEVNDAQNYLQTKGLNIEQC 190
BLAST of SMED30004833 vs. TrEMBL
Match: A0A0L8HCR5 (DUF4371 domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22017424mg PE=4 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 7.339e-13 Identity = 28/97 (28.87%), Postives = 47/97 (48.45%), Query Frame = -1 Query: 235 TEQMLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW*IKVS 522 T +L +L+ + I+ C G+ ++NA G + R + + K V C NH N A V+ A +++TFFG +++ F S S W I + Sbjct: 376 TSMILQQLEMDDINIQDCRGQTFDNASVKAGHRSSVQARITEVNPKIVFVPCTNHSLNLAAVYAASVGADSVTFFGTLDRFFSFFSASTHRWDISIE 472 HSP 2 Score: 46.9802 bits (110), Expect = 7.339e-13 Identity = 39/137 (28.47%), Postives = 73/137 (53.28%), Query Frame = -2 Query: 615 DHETSQTHLVAFDVWKEMAVPVGAG*MIDGDK------DVAK*RNVLKRIGDEFV----QNFALRRHDEKL-LEEFDINSGIFIELMKVMGKYD----ENTS**TDTQSEFIS-----LQAAIIYDFQNEKYFTIIF 965 ++E+S H AF WKE+ + + G +I ++ + K + +L R D + QN ALR H E + +E+ + N G F+EL++++GK+D E+ ++EF++ ++ +I + + KY+ IIF Sbjct: 196 ENESSPVHENAFTQWKELEIRLFKGMVIGKEQQTLVENETKKWKQILTRFLDIIMFLAKQNLALRGHREDIRVEKANENEGNFLELVQLIGKFDPVLREHL-----VKNEFVNVLGDRVRKKVIDEVKQSKYYCIIF 327 HSP 3 Score: 23.0978 bits (48), Expect = 7.339e-13 Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -3 Query: 1004 AFYCFCCGLFNPD 1042 A +CFCC LF+ D Sbjct: 170 AVFCFCCKLFSSD 182 HSP 4 Score: 21.1718 bits (43), Expect = 7.339e-13 Identity = 7/12 (58.33%), Postives = 10/12 (83.33%), Query Frame = -3 Query: 575 TNINQTSHILRY 610 ++INQ S +LRY Sbjct: 334 SHINQISQVLRY 345
BLAST of SMED30004833 vs. TrEMBL
Match: A0A1Y1NBI7 (DUF4371 domain-containing protein (Fragment) OS=Photinus pyralis OX=7054 PE=4 SV=1) HSP 1 Score: 79.337 bits (194), Expect = 1.902e-12 Identity = 41/106 (38.68%), Postives = 59/106 (55.66%), Query Frame = -1 Query: 199 MLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEKF-CLISLSPCSW*IKVSNEFVRLTRVPNT 513 +L KLK GL I C G+GY+NA M G H G+ R S++ KA + C NH N A +H A+ VN++TFFG +++ SLS W + + L R+ +T Sbjct: 62 ILEKLKSDGLDIMNCRGQGYDNAAVMKGQHSGVQKRISDVNPKAQFIACSNHSLNLAGIHAAQAEVNSVTFFGTLDRLHSFFSLSTHRWDVLSTTTGTSLKRLVDT 167
BLAST of SMED30004833 vs. Ensembl Cavefish
Match: ENSAMXT00000025204.2 (pep primary_assembly:Astyanax_mexicanus-2.0:12:7364240:7371214:-1 gene:ENSAMXG00000024490.2 transcript:ENSAMXT00000025204.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 53.1434 bits (126), Expect = 6.238e-7 Identity = 30/92 (32.61%), Postives = 47/92 (51.09%), Query Frame = -1 Query: 250 TEQMLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW 522 TE +L L+ L I C G+ Y+N M G G+ R + +KA C+ C +HK N A+ V + +FFG++++ + L S S W Sbjct: 303 TEVLLDHLQKHNLSISDCRGQSYDNGSNMMGQKQGVRARILQLNNKALCIPCSSHKLNLVVADAAKSSVLSTSFFGVLQRLYNLFSSSVQRW 394
BLAST of SMED30004833 vs. Ensembl Cavefish
Match: ENSAMXT00000036272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:11935414:11938921:1 gene:ENSAMXG00000041670.1 transcript:ENSAMXT00000036272.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 52.7582 bits (125), Expect = 7.638e-7 Identity = 28/91 (30.77%), Postives = 45/91 (49.45%), Query Frame = -1 Query: 244 MLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW*I 513 +L KL+ G+ E C G+ Y+N M G G+ R + +AA V C H N A+ +A+++FG +++ FC S + W I Sbjct: 386 VLEKLRALGIPFEDCRGQAYDNGANMKGKRQGVQARLLQLNPRAAFVPCAAHTMNLVISDAAKSSTDAISYFGYLQRLFCFFSAATQRWDI 476
BLAST of SMED30004833 vs. Ensembl Cavefish
Match: ENSAMXT00000055782.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001476.1:1798:4262:-1 gene:ENSAMXG00000042376.1 transcript:ENSAMXT00000055782.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.8322 bits (120), Expect = 2.607e-6 Identity = 32/86 (37.21%), Postives = 43/86 (50.00%), Query Frame = -1 Query: 250 KLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIE-KFCLISLSPCSW 504 +LK+ L I C G+ YNN M G G+ R I KA CV C +H N A+ V ++TFFGI++ + L S S W Sbjct: 336 ELKHMKLNIADCRGQSYNNGSNMMGHKQGVQKRVLEINQKALCVPCSSHSLNLVVADAAKSSVLSVTFFGILQWLYNLFSSSVHRW 421
BLAST of SMED30004833 vs. Ensembl Cavefish
Match: ENSAMXT00000044961.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000032.1:419978:423709:1 gene:ENSAMXG00000036481.1 transcript:ENSAMXT00000044961.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 50.8322 bits (120), Expect = 2.810e-6 Identity = 29/92 (31.52%), Postives = 46/92 (50.00%), Query Frame = -1 Query: 250 TEQMLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW 522 TE +L L+ L I C G+ Y+N M G G+ R + +KA C+ C +H N A+ V + +FFG++++ + L S S W Sbjct: 357 TEVLLDHLQKHNLSISDCRGQSYDNGSNMMGQKQGVQARILQLNNKALCIPCSSHTLNLVVADAAKSSVLSTSFFGVLQRLYNLFSSSVQRW 448
BLAST of SMED30004833 vs. Ensembl Cavefish
Match: ENSAMXT00000033195.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:11277158:11279506:-1 gene:ENSAMXG00000030798.1 transcript:ENSAMXT00000033195.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 49.2914 bits (116), Expect = 9.573e-6 Identity = 32/109 (29.36%), Postives = 49/109 (44.95%), Query Frame = -1 Query: 199 TEQMLYKLKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEK-FCLISLSPCSW*IKVSNEFVRLTRVPNT 522 +E ++ L+ G+ ++ C G+GY+N M+G G+ + A C H N VH AE TFFG + + + L S SP W I L R+ +T Sbjct: 340 SEMIIEALQSNGIPLQECRGQGYDNGANMSGKVKGVQAQILKANPLATFSPCATHTLNLVGVHAAESCPEVATFFGSVNRLYNLFSASPERWAILKEKTGCSLHRLSDT 448
BLAST of SMED30004833 vs. Planmine SMEST
Match: SMESG000069312.1 (SMESG000069312.1) HSP 1 Score: 56.6102 bits (135), Expect = 5.454e-9 Identity = 47/146 (32.19%), Postives = 68/146 (46.58%), Query Frame = -2 Query: 615 HETSQTHLVAFDVWKEMAVPVGAG*MIDGD------KDVAK*RNVLKRIGD--EFV--QNFALRRHDEKLLEEFDINSGIFIELMKVMGKYDENTS**TD---------------TQSEFISLQAA-----IIYDFQNEKYFTIIF 962 HE S TH +F WKE + +G ID + + K R+VLKR+ +F+ QN ALR H E L N G F+ +K++ +YD S D TQ+E ISL A+ ++ D + KY+ I+F Sbjct: 225 HENSPTHRASFTAWKEAERRIMSGKGIDAELQAQMQNEKQKWRDVLKRLLSCVKFLASQNLALRGHAESLATNNSSNVGNFLATLKLLAEYDPLLSNHIDHARQNPGSVSYLSPQTQNESISLLASKVRNKLLNDIKRNKYYGILF 370 HSP 2 Score: 22.7126 bits (47), Expect = 5.454e-9 Identity = 7/8 (87.50%), Postives = 7/8 (87.50%), Query Frame = -3 Query: 1013 YCFCCGLF 1036 YCFCC LF Sbjct: 189 YCFCCLLF 196 HSP 3 Score: 45.0542 bits (105), Expect = 4.408e-6 Identity = 29/85 (34.12%), Postives = 42/85 (49.41%), Query Frame = -1 Query: 250 LKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEKFCL-ISLSPCSW 501 L+ L ++ C + Y+NA M+G G+ R N +A V C NH N A +H A++ A+TFF I+ L S S W Sbjct: 427 LEADNLRLDDCRSQCYDNAALMSGHISGLQQRICNRNPRAIFVNCNNHSLNLAGMHSAKQDAVAVTFFRTIKSIYLYFSRSTLRW 511 HSP 4 Score: 24.6386 bits (52), Expect = 4.408e-6 Identity = 11/28 (39.29%), Postives = 19/28 (67.86%), Query Frame = -3 Query: 170 LVNKSVKRVCETH*SSKHDAVASNTEGL 253 +++ +VKR CET S++ DAV + G+ Sbjct: 517 VISNTVKRECETRWSARADAVKAVHNGV 544
BLAST of SMED30004833 vs. Planmine SMEST
Match: SMESG000049700.1 (SMESG000049700.1) HSP 1 Score: 46.9802 bits (110), Expect = 2.540e-6 Identity = 30/85 (35.29%), Postives = 43/85 (50.59%), Query Frame = -1 Query: 250 LKYGGLYIEYCSGEGYNNAPTMTGIHIGMGMRFSNIYSKAACVLCMNHK*N*ARVHCAEKVVNALTFFGIIEKFCL-ISLSPCSW 501 L+ L ++ C + Y+NA M+G G+ R N +A V C N+ N A +H A++ A+TFFG IE L S S W Sbjct: 317 LEADNLRLDDCRSQCYDNAALMSGHISGLQQRICNRNPRAIFVNCNNYSLNLAGMHSAKQDAVAVTFFGTIESIYLYFSRSTLRW 401 HSP 2 Score: 23.8682 bits (50), Expect = 2.540e-6 Identity = 10/28 (35.71%), Postives = 19/28 (67.86%), Query Frame = -3 Query: 170 LVNKSVKRVCETH*SSKHDAVASNTEGL 253 +++ +VKR C+T S++ DAV + G+ Sbjct: 407 VISNTVKRECDTRWSARADAVKAVHNGV 434 The following BLAST results are available for this feature:
BLAST of SMED30004833 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004833 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004833 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30004833 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30004833 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004833 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004833 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004833 ID=SMED30004833|Name=SMED30004833|organism=Schmidtea mediterranea sexual|type=transcript|length=1533bpback to top Annotated Terms
The following terms have been associated with this transcript:
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