SMED30004814
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004814 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30004814 aligns in the following genomic locations:
Homology
BLAST of SMED30004814 vs. Planmine SMEST
Match: SMESG000040945.1 (SMESG000040945.1) HSP 1 Score: 840.491 bits (2170), Expect = 0.000e+0 Identity = 497/499 (99.60%), Postives = 497/499 (99.60%), Query Frame = 2 Query: 35 MESIELNESITDEVASNKQEIMEICVYGTCILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSALIIANMYHNFNNRNFFEKHKIPNQSILYLICXXXXXXXXXXXXXXXMKEVLLKENSSVGKEXXXXXXXXXXXXXXXXXXEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMAKSETSNLYSALESLFELNSNLVYKXXXXXXXXXXXXXXNPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSXXXXXXXXXXXXYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYSHSAVSGQFHVQLFESGFNHLGMIFSVLHRIHGAXXXXXXXXXXXXNKPALYTCNL 1531 MESIELNESITDEVASNKQEIMEI VYGTCILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSS LIIANMYHNFNNRNFFEKHKIPNQSILYLICLPSLVLILVGLSIVIMKEVLLKENSSVGKEIFSVSSLILFLSILSLLIEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMAKSETSNLYSALESLFELNSNLVYKIIIIFNISCIFFLINPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSFIFIVAICVCIIYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYSHSAVSGQFHVQLFESGFNHLGMIFSVLHRIHGAIVFVYYFKYYIINKPALYTCNL Sbjct: 1 MESIELNESITDEVASNKQEIMEISVYGTCILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSVLIIANMYHNFNNRNFFEKHKIPNQSILYLICLPSLVLILVGLSIVIMKEVLLKENSSVGKEIFSVSSLILFLSILSLLIEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMAKSETSNLYSALESLFELNSNLVYKIIIIFNISCIFFLINPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSFIFIVAICVCIIYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYSHSAVSGQFHVQLFESGFNHLGMIFSVLHRIHGAIVFVYYFKYYIINKPALYTCNL 499
BLAST of SMED30004814 vs. Planmine SMEST
Match: SMESG000051898.1 (SMESG000051898.1) HSP 1 Score: 91.2781 bits (225), Expect = 3.471e-19 Identity = 123/467 (26.34%), Postives = 228/467 (48.82%), Query Frame = 2 Query: 125 ILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSALIIANMYHNFNNRNFFEKHKIPNQSILYLICXXXXXXXXXXXXXXXMKEVLLKENSSVGKEXXXXXXXXXXXXXXXXXXEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMA-KSETSNLYSALESLFELNSNL---VYKXXXXXXXXXXXXXXNPLVYHFKNLQ----NSKVSQLV--QEEIPEDEEQPKLPN------SSLILSXXXXXXXXXXXXYDWTSKQSLE--LINLLIVSTYIASFMHIYFILKNIQKCPRK-DKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYSHSAV--SGQFHVQLFESGFNHLGMIFSVLHRIHG 1462 ++TFS G+L Y + +L+ +S + V++ PL + G+ N++ LI+ +Y N RN F K + NQ ILY C+P+ + ++ L I ++K + + E + +F +S+I F+ I+ + E N D GE W++N +NM L + SLV N+++ ++Q+ + +N S+ +S NL + + L ++ +YK I++F + F+ L ++ LQ + K + L E++ + ++ L S+L+ +F+ I SK+ LE LI + + S YI+ F++KN+ ++ + V +WLGL GQ + A A I LM + + Y +++Y Q++ Q++ ++E+ W K + + ++ +N ++F L+Y + G ++LF F + G IFSVL RIHG Sbjct: 21 VITFSIGLLITYGWNTLTLASNSFLEFVWA-PLYICGSVFNLLVVFLILFALYMNHKYRNLFNKCPMENQDILYYFCIPATIFVISTLIITLVKSLYIAECNGKMIVLFIFTSVIYFVVIVVFIAETNSVFDFGESWALNFVNMMVSLIIVSSLVFNIVFNTSLEQMDYFAAVINKSLINQSCIDNLPFIAQRTYYLWVSVRIFLYKSIVLFGLFSTVFIFKSLYRNYLYLQFYKYDGKTNDLKSNDEDVNRNSQKNNLKKLYLKIFSTLLFGILFLAINIAVYI---RSKEYLEHILIGMEMFS-YISMIGLTIFMMKNLIFTDFSISRIYVFNEVIIWLGLCGQLVHCFAVIAAYIYLMYNEVNAIYIAILIKYVLQILSNIVQSVFEAILVEKLWNNKIGKNFSTMAFGAFYIAVNNFVYIFTDLILVYKEFLLPFEGSVKLKLFVCLFFNFGNIFSVLQRIHG 482
BLAST of SMED30004814 vs. Planmine SMEST
Match: SMESG000009259.1 (SMESG000009259.1) HSP 1 Score: 85.5001 bits (210), Expect = 2.304e-17 Identity = 114/464 (24.57%), Postives = 210/464 (45.26%), Query Frame = 2 Query: 83 NKQEIMEICVYGTCILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSALIIANMYHNFNNRNFFEKHKIPNQSILYLICXXXXXXXXXXXXXXXMKEVLLKENSSVGKEXXXXXXXXXXXXXXXXXXEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSM--------AKSETSNLYSALESLFELNSNLVYKXXXXXXXXXXXXXXNPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSXXXXXXXXXXXXYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYSHSAVSGQFHVQLFESGFNHLGMIFSVLH 1450 K+ ++EIC Y + T GILF Y + + L ++ FS+ +++ G +++++ + N Y N NRNFF K+ + I YLIC+P L+L ++ V MK + +SVS IL L IL L E + Y+D+GEKW I +N TT+L T I+++ +I+ ++Q+ I + ++ +++ T + F+ L+ K II+F S F I PL+ ++NL+ + + + L +I++ I +++K + E+ ++++ S M I+ I K + P K S V +++G+ GQ +++ A + ++ SD Y + + F + +I ++W+ N W+ + C + + +N F F Y + S F V+ F G FS+L+ Sbjct: 7 GKRHLLEICSYNLGVYTLVIGILFMYGYNSVFLEQPPLYEPRFSNTVSIFGVTSHIINIFFLFYNWYINIRNRNFFSPIKLDSVLIFYLICVPFLILAILYSIFVTMKLFFSPWCMARADVAYSVSCNILALLILIHLAELSFYRDTGEKWIITYVNFTTLLITFINVMVFVIFQFSLEQINTLISIYDFNISYNPCSMDSRNFTGSFMRNFLFFFKDWEKLMLKGIIVFGTSSSVFFIGPLMRQYRNLKKFNKASIPSIAVRNSRFSKVLAIPFIIIALAVFSVFIFINITQFSTK-NYEI--MMVIQHAFGSCMIIWQI-KTLLHWPSNGKSISSLEVLLYVGVCGQSLKNTALFEQFLHQIVFYSDYIYLINDFHFLLSIFYSFLITSFICLLIGKRWEVTNGNFWFITILCLVLISINNAISSFQDF---YKRNLFSVFFRVRYFNHALFIHGTTFSILY 463
BLAST of SMED30004814 vs. Planmine SMEST
Match: SMESG000035551.1 (SMESG000035551.1) HSP 1 Score: 83.9593 bits (206), Expect = 8.387e-17 Identity = 119/473 (25.16%), Postives = 223/473 (47.15%), Query Frame = 2 Query: 83 NKQEIMEI--CVYGTCILTFSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSALIIANMYHNFNNRNFFEKHKIPNQSILYLICXXXXXXXXXXXXXXXMKEVLLKENSSVGKEXXXXXXXXXXXXXXXXXXEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMAKSETSNLYSALESLFELNSNLVYKXXXXXXXXXXXXXXNPLVYHFKNLQNS--KVSQLVQEE-----IPEDEEQPKLPNSSLILSXXXXXXXXXXXXYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYS---HSAVSGQFHVQLFESGFNHLGMIFSVLHRIHGA 1465 NK +I+ I +Y I+ FS G+ Y +AQ+L + + F PL+V + TN+ +I+ ++Y NF RNF E K IL+LI LP VL ++ +++ +K+ L ++ F+ +S++ F+ L LLIE D+GE ++L+ +T IL+ + ++N +I+ + IDQ++N+ ++N + + + Y L++ + + + I I + L+Y ++ L+ S V + VQ E + +L S S I I+ I + I+ K ++L +I S + +YF ++++ ++ K + I + F+ +GL G I I + I + N I++Y T++ + Q + I + F K + CP+F + S +F+ Y++ +S + F QL E GF + G +F + R H + Sbjct: 20 NKFKILLIIKVIYTIFIIYFSLGLYLTYKHIAQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEILFLIILPLFVLSIIIMALGSIKDT-LSDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVV-------SLIYMYEPLKQSFNIVERFVQYENEFGSLLNTIPPKRLSRFSAGFSLIIIILITLSSIFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIIDHWFITIGLIGSLIHCI----SSIDALAYNLHGWSVFYIIKYVTEIFQIVTQTTYIFMFINLENYGFFKNRLMEII-CPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVEIGFYNFGNMFMIFMRFHAS 479
BLAST of SMED30004814 vs. Planmine SMEST
Match: SMESG000040960.1 (SMESG000040960.1) HSP 1 Score: 59.6918 bits (143), Expect = 3.409e-9 Identity = 107/429 (24.94%), Postives = 200/429 (46.62%), Query Frame = 2 Query: 233 GAFTNVMSSALIIANMYHNFNNRNFFEKHKIPNQSILYLICXXXXXXXXXXXXXXXMKEVLLKENSSVGKEXXXXXXXXXXXXXXXXXXEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVS-MAKSETSNLYSALESLFELNSNLV---YKXXXXXXXXXXXXXXNPLVYHFKNLQNSKVSQLVQEEIPEDEEQPKLPNSSLILSXXXXXXXXXXXXY------DWTSKQSLEL-----INLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYS--FLSCPLFLVCSNTGFMFLSFYLLYSH--SAVSGQFHVQLFESGFNHLGMIFSVLHRIHG 1462 G+ N++S+++II N Y N NRN F K ++ N IL+ C P + I+ L I + ++ L G+ IF+ ++ I +++++ L + + D GE+W IN NM+ I IIS + ++ + ++Q+ + LN++ S +L + +++ L L+ K +IF C LI P ++H+ +L N K+ Q + + + + N SLI++ F + C + WT S+ L I LLI S Y S + C K+ + +W+GL GQF+ S+ I + + + +Y +++I+ Q I + ++E +W+ N+KL + ++ LFL+ +N L+ + + + ++F + M+F VL R+HG Sbjct: 60 GSVMNIVSASIIILNEYLNHLNRNHFYKLRVLNSEILHTFCFPLVSFIIGSLLISTINDIYLIRCIGYGRMIFASTNSIYYITVMIFLAKYHFVCDDGEEWIINFSNMSAIFIIIISQIFYILVSISVEQITLIVPILNITDFDTSCIQHLNTISKTIDNLTGRLLMPMMKAWLIFGTLCCTLLIMPFLHHYSSLNNHKIEQSIIKNSSFKGVKHDISNFSLIVTLAFGIGFLSCKFFFYYYHTYWTMILSVILEINISIILLICSLYATSEAATW------PVCSV--KLFQFNNAIVWIGLIGQFVFSVTMIMNSISHLSTEFSILGLCVLFEYLSKMIQISVQFILESLLLETQWE--NRKLEKNSKLINAFLFLMINNLCLTILNIARINDNILPPLKDPISFKIFFIFTTNFSMVFCVLQRLHG 478 The following BLAST results are available for this feature:
BLAST of SMED30004814 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004814 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004814 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004814 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004814 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004814 ID=SMED30004814|Name=SMED30004814|organism=Schmidtea mediterranea sexual|type=transcript|length=1783bpback to top protein sequence of SMED30004814-orf-1 >SMED30004814-orf-1 ID=SMED30004814-orf-1|Name=SMED30004814-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=500bp MESIELNESITDEVASNKQEIMEICVYGTCILTFSFGILFNYAFVAQSLSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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