60S ribosomal protein L27a
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004808 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 174.481 bits (441), Expect = 2.672e-56 Identity = 102/141 (72.34%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGM+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 144
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 130.568 bits (327), Expect = 9.333e-40 Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 M+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 87
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 130.568 bits (327), Expect = 9.333e-40 Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 M+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 87
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 130.568 bits (327), Expect = 9.333e-40 Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 M+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 87
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 130.568 bits (327), Expect = 9.333e-40 Identity = 56/87 (64.37%), Postives = 77/87 (88.51%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 M+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 1 MKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 87
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069681:2070607:-1 gene:WBGene00021350.1 transcript:Y37E3.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8) HSP 1 Score: 154.451 bits (389), Expect = 6.733e-49 Identity = 97/140 (69.29%), Postives = 118/140 (84.29%), Query Frame = 1 Query: 28 VKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 ++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGGQHHHRI+ DK+HPGYFGKVGMR +HL KN+ +CP++N+E+LWSLV E + GK+PVID K GYFK+LGKG+LP+ P+IVKA+FFS +AE+KIK GGAC Sbjct: 5 LRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGQHHHRINRDKYHPGYFGKVGMRVFHLNKNQHYCPTVNVERLWSLVPQ---EVRDKATGGKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGAC 141
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069683:2070209:-1 gene:WBGene00021350.1 transcript:Y37E3.8b.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8) HSP 1 Score: 107.457 bits (267), Expect = 3.918e-31 Identity = 50/87 (57.47%), Postives = 66/87 (75.86%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MR +HL KN+ +CP++N+E+LWSLV E + GK+PVID K GYFK+LGKG+LP+ P+IVKA+FFS +AE+KIK GGAC Sbjct: 1 MRVFHLNKNQHYCPTVNVERLWSLVPQ---EVRDKATGGKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGAC 84
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Match: Y37E3.8 (pep chromosome:WBcel235:I:2069683:2070574:-1 gene:WBGene00021350.1 transcript:Y37E3.8b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y37E3.8) HSP 1 Score: 107.457 bits (267), Expect = 3.918e-31 Identity = 50/87 (57.47%), Postives = 66/87 (75.86%), Query Frame = 1 Query: 187 MRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MR +HL KN+ +CP++N+E+LWSLV E + GK+PVID K GYFK+LGKG+LP+ P+IVKA+FFS +AE+KIK GGAC Sbjct: 1 MRVFHLNKNQHYCPTVNVERLWSLVPQ---EVRDKATGGKSPVIDCTKLGYFKVLGKGLLPETPLIVKARFFSHEAEQKIKKAGGAC 84
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0345285) HSP 1 Score: 155.606 bits (392), Expect = 2.939e-49 Identity = 99/145 (68.28%), Postives = 119/145 (82.07%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMR++HLR+ F P +N++KLWSLV + + ++ KAPVIDL+K GY+KLLG+G LP PVIVKAK+FS++AE+KIK GG C Sbjct: 1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVC 145
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0077452) HSP 1 Score: 155.606 bits (392), Expect = 2.939e-49 Identity = 99/145 (68.28%), Postives = 119/145 (82.07%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMR++HLR+ F P +N++KLWSLV + + ++ KAPVIDL+K GY+KLLG+G LP PVIVKAK+FS++AE+KIK GG C Sbjct: 1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVC 145
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Match: RpL27A (gene:FBgn0285948 transcript:FBtr0335136) HSP 1 Score: 155.606 bits (392), Expect = 2.939e-49 Identity = 99/145 (68.28%), Postives = 119/145 (82.07%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MS IK KKTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMR++HLR+ F P +N++KLWSLV + + ++ KAPVIDL+K GY+KLLG+G LP PVIVKAK+FS++AE+KIK GG C Sbjct: 1 MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRNFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPARPVIVKAKYFSKKAEDKIKKAGGVC 145
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552]) HSP 1 Score: 178.333 bits (451), Expect = 5.554e-58 Identity = 106/141 (75.18%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL+KN++FCP++N++KLW+LVS++T + +N EG AP+I+ + AGY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 4 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGAC 144
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552]) HSP 1 Score: 178.333 bits (451), Expect = 5.559e-58 Identity = 106/141 (75.18%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL+KN++FCP++N++KLW+LVS++T + +N EG AP+I+ + AGY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 3 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGAC 143
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Match: rpl27a (ribosomal protein L27a [Source:NCBI gene;Acc:100170552]) HSP 1 Score: 178.333 bits (451), Expect = 6.825e-58 Identity = 106/141 (75.18%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL+KN++FCP++N++KLW+LVS++T + +N EG AP+I+ + AGY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 6 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNPEGPAPIINAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGAC 146
BLAST of 60S ribosomal protein L27a vs. Ensembl Mouse
Match: Rpl27a (ribosomal protein L27A [Source:MGI Symbol;Acc:MGI:1347076]) HSP 1 Score: 174.096 bits (440), Expect = 2.003e-56 Identity = 102/141 (72.34%), Postives = 128/141 (90.78%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIK VGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGVAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGAC 144
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P47830|RL27A_XENLA (60S ribosomal protein L27a OS=Xenopus laevis OX=8355 GN=rpl27a PE=2 SV=2) HSP 1 Score: 179.874 bits (455), Expect = 7.328e-58 Identity = 107/141 (75.89%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 K++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL+KN++FCP++N++KLW+LVS++T + +N EG AP+ID + AGY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 4 KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKKNQSFCPTINLDKLWTLVSEQTRLNHAKNLEGPAPIIDAVHAGYYKVLGKGKLPKQPVIVKAKFFSRKAEEKIKSVGGAC 144
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|Q56K03|RL27A_BOVIN (60S ribosomal protein L27a OS=Bos taurus OX=9913 GN=RPL27A PE=2 SV=3) HSP 1 Score: 174.866 bits (442), Expect = 7.030e-56 Identity = 102/141 (72.34%), Postives = 128/141 (90.78%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIK VGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGAC 144
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P18445|RL27A_RAT (60S ribosomal protein L27a OS=Rattus norvegicus OX=10116 GN=Rpl27a PE=1 SV=3) HSP 1 Score: 174.481 bits (441), Expect = 1.089e-55 Identity = 102/141 (72.34%), Postives = 128/141 (90.78%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGMRHYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIK VGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKNGVAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGAC 144
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|Q5REY2|RL27A_PONAB (60S ribosomal protein L27a OS=Pongo abelii OX=9601 GN=RPL27A PE=2 SV=3) HSP 1 Score: 174.481 bits (441), Expect = 1.283e-55 Identity = 102/141 (72.34%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGM+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 144
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Match: sp|P46776|RL27A_HUMAN (60S ribosomal protein L27a OS=Homo sapiens OX=9606 GN=RPL27A PE=1 SV=2) HSP 1 Score: 174.481 bits (441), Expect = 1.283e-55 Identity = 102/141 (72.34%), Postives = 129/141 (91.49%), Query Frame = 1 Query: 25 KVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 +++KTRKLRGHVSHGHGR+GKHRKHPGGRGNAGG HHHRI+ DK+HPGYFGKVGM+HYHL++N++FCP++N++KLW+LVS++T +N G AP+ID++++GY+K+LGKG LPK PVIVKAKFFSR+AEEKIKSVGGAC Sbjct: 4 RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKSVGGAC 144
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: D3PHZ9 (60S ribosomal protein L27a OS=Lepeophtheirus salmonis OX=72036 GN=RL27A PE=2 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 7.487e-62 Identity = 115/145 (79.31%), Postives = 136/145 (93.79%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSK+K KTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRI+ DK+HPGYFGK+GMRHYHL+KN ++CP++N++++WSLVS+KTY+ Y+++ EGKAPVID +K GYFK+LGKGVLPKVPVIVKA+FFSRQAEEKIK VGGAC Sbjct: 1 MSKLKPTKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRINFDKYHPGYFGKIGMRHYHLKKNVSWCPTVNLDRVWSLVSEKTYQKYRDDKEGKAPVIDCVKHGYFKVLGKGVLPKVPVIVKARFFSRQAEEKIKKVGGAC 145
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A4S2LM24 (Ribosomal_L18e/L15P domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007592 PE=3 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 1.891e-61 Identity = 119/145 (82.07%), Postives = 134/145 (92.41%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSKIK KKTRKLRGHVSHGHGRVGKHRKHPGGRGNAG +HHHRI+MDK+HPGY GKVGMRHYH +KN FCP++NIEK+WSLVS TYE YK++A+GKAPVID LK GYFK+LGKGVLPKVPVIVKA+FFSR+AEEKI++VGGAC Sbjct: 32 MSKIKPKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGLEHHHRINMDKYHPGYIGKVGMRHYHYKKNPYFCPAINIEKIWSLVSQDTYEKYKDHADGKAPVIDCLKKGYFKVLGKGVLPKVPVIVKARFFSRRAEEKIRAVGGAC 176
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A068YJK8 (Large subunit ribosomal protein L27Ae OS=Echinococcus multilocularis OX=6211 GN=EmuJ_001015500 PE=3 SV=1) HSP 1 Score: 193.741 bits (491), Expect = 9.502e-61 Identity = 112/145 (77.24%), Postives = 133/145 (91.72%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSKIK KKTRKLRGHVSHG GRVGKHRKHPGGRGNAGG+HHHRI+ +K+HPGYFGK+GM HYHLR+N +CP++N++KLWSLVSD TYE YK + +GKAPVID ++ GYFK+LGKG+LPKVPVIVKA+FFSR+AEE+IK+VGGAC Sbjct: 1 MSKIKAKKTRKLRGHVSHGMGRVGKHRKHPGGRGNAGGEHHHRINFNKYHPGYFGKIGMPHYHLRRNPYYCPTINLDKLWSLVSDATYEKYKNSKDGKAPVIDCVRKGYFKVLGKGILPKVPVIVKARFFSRRAEERIKAVGGAC 145
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A068WCE3 (Large subunit ribosomal protein L27Ae OS=Echinococcus granulosus OX=6210 GN=EgrG_001015500 PE=3 SV=1) HSP 1 Score: 192.2 bits (487), Expect = 4.033e-60 Identity = 111/145 (76.55%), Postives = 133/145 (91.72%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSKIK KKTRKLRGHVSHG GRVGKHRKHPGGRGNAGG+HHHRI+ +K+HPGYFGK+GM HYHL++N +CP++N++KLWSLVSD TYE YK + +GKAPVID ++ GYFK+LGKG+LPKVPVIVKA+FFSR+AEE+IK+VGGAC Sbjct: 1 MSKIKAKKTRKLRGHVSHGMGRVGKHRKHPGGRGNAGGEHHHRINFNKYHPGYFGKIGMPHYHLKRNPYYCPTINLDKLWSLVSDATYEKYKNSKDGKAPVIDCVRKGYFKVLGKGILPKVPVIVKARFFSRRAEERIKAVGGAC 145
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Match: A0A0R3U1D5 (Ribosomal_L18e/L15P domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS167 PE=3 SV=2) HSP 1 Score: 191.43 bits (485), Expect = 6.458e-60 Identity = 111/145 (76.55%), Postives = 133/145 (91.72%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSK K KKTRKLRGHVSHG GRVGKHRKHPGGRGNAGG+HHHRI+ DK+HPGYFGK+GM HYHL++N +CP++N++KLWSLVSD TYE YK++ +GKAPVID ++ GYFK+LGKGVLPK+PVIVKA+FFSR+AEE+IK+VGGAC Sbjct: 1 MSKSKPKKTRKLRGHVSHGMGRVGKHRKHPGGRGNAGGEHHHRINFDKYHPGYFGKIGMPHYHLKRNPYYCPTINLDKLWSLVSDATYEKYKKSTDGKAPVIDCVRKGYFKVLGKGVLPKIPVIVKARFFSRRAEERIKAVGGAC 145
BLAST of 60S ribosomal protein L27a vs. Ensembl Cavefish
Match: RPL27A (ribosomal protein L27a [Source:HGNC Symbol;Acc:HGNC:10329]) HSP 1 Score: 166.392 bits (420), Expect = 5.624e-54 Identity = 74/107 (69.16%), Postives = 93/107 (86.92%), Query Frame = 1 Query: 127 QHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 HHHRI+ DK+HPGYFGKVGMRHYHL++N FCP++N++KLW+LVS++T + + +G APVID+++AGYFK+LGKG LPK PVIVKAKFFSR+AEEKIK VGGAC Sbjct: 1 MHHHRINFDKYHPGYFGKVGMRHYHLKRNTVFCPTINLDKLWTLVSEQTRVNHAKKLDGPAPVIDVVRAGYFKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGAC 107
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Match: RPL28 (Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance [Source:SGD;Acc:S000003071]) HSP 1 Score: 135.576 bits (340), Expect = 3.968e-42 Identity = 65/111 (58.56%), Postives = 85/111 (76.58%), Query Frame = 1 Query: 118 AGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLV-SDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 AGGQHHHRI+MDK+HPGYFGKVGMR++H ++ + P LN++KLW+L+ DK + K ++ APVID L AGY K+LGKG +P VPVIVKA+F S+ AEEKI++ GG Sbjct: 35 AGGQHHHRINMDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVIDTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVV 145
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Match: YGL102C_mRNA (Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein [Source:SGD;Acc:S000003070]) HSP 1 Score: 55.4546 bits (132), Expect = 3.913e-11 Identity = 46/126 (36.51%), Postives = 64/126 (50.79%), Query Frame = -1 Query: 67 STHGFNLFFCLS*KEFRLDDNWNFWQNSFTKQFKVASF*KIDYWXXXXXXXXXXXXXXV-TD*AP*LFNV*RWTKCSIFS*MIMPHTNFTEVTRMEFIHMNSMMMLTTCITTTSRMLPVFTNSTMT 441 +T + FF E DDNWN W ++ T+ V ++ WS F VSLV D P V W + + HTN TE+T M FIH+NS++MLTT T+T+ +L V T++T T Sbjct: 10 TTSSSDFFFSQLGDESSFDDNWNIWNSTLTQDLTVTGCQSVNNWSSFLRSRFQVLVSLVFWDQCPQFVQVQDWLPEMSLLLVEVSHTNLTEITWMVFIHVNSVVMLTTGHTSTTGVLSVLTDTTFT 135
BLAST of 60S ribosomal protein L27a vs. Ensembl Nematostella
Match: EDO47596 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLF6]) HSP 1 Score: 170.629 bits (431), Expect = 1.071e-55 Identity = 77/111 (69.37%), Postives = 97/111 (87.39%), Query Frame = 1 Query: 115 NAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 NAGGQHHHRI+ DK+HPGYFGKVGMRH+HL +N PS+N++K+WSLVS++T + YK +G PVID++KAGY+K+LGKG+LPK PVIVKAKFFSR+AE+KIK+VGGAC Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRHFHLTRNAYHKPSINLDKVWSLVSEQTRQNYKNKKDGPVPVIDVVKAGYYKVLGKGLLPKQPVIVKAKFFSRRAEDKIKAVGGAC 130
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Match: RPL27A (ribosomal protein L27a [Source:NCBI gene;Acc:101160900]) HSP 1 Score: 177.563 bits (449), Expect = 7.793e-58 Identity = 79/111 (71.17%), Postives = 98/111 (88.29%), Query Frame = 1 Query: 115 NAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 NAGG HHHRI+ DK+HPGYFGKVGMRHYHL++N T+CP++N++KLW+LVS++T Y + +G APVID ++AGY+K+LGKG LPK PVIVKAKFFSRQAEEKIK+VGGAC Sbjct: 34 NAGGLHHHRINFDKYHPGYFGKVGMRHYHLKRNTTYCPTINLDKLWTLVSEQTRVNYGKKPDGPAPVIDAVRAGYYKVLGKGKLPKQPVIVKAKFFSRQAEEKIKAVGGAC 144
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Match: RPL27A (ribosomal protein L27a [Source:NCBI gene;Acc:101160900]) HSP 1 Score: 153.295 bits (386), Expect = 1.502e-48 Identity = 68/96 (70.83%), Postives = 85/96 (88.54%), Query Frame = 1 Query: 160 HPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 HPGYFGKVGMRHYHL++N T+CP++N++KLW+LVS++T Y + +G APVID ++AGY+K+LGKG LPK PVIVKAKFFSRQAEEKIK+VGGAC Sbjct: 25 HPGYFGKVGMRHYHLKRNTTYCPTINLDKLWTLVSEQTRVNYGKKPDGPAPVIDAVRAGYYKVLGKGKLPKQPVIVKAKFFSRQAEEKIKAVGGAC 120
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Match: SMESG000005086.1 (SMESG000005086.1) HSP 1 Score: 243.432 bits (620), Expect = 4.978e-84 Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC Sbjct: 1 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 145
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Match: SMESG000034631.1 (SMESG000034631.1) HSP 1 Score: 225.713 bits (574), Expect = 5.868e-77 Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 1 Query: 13 MSKIKVKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHMDKFHPGYFGKVGMRHYHLRKNRTFCPSLNIEKLWSLVSDKTYETYKENAEGKAPVIDLLKAGYFKLLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 447 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIH DKFHPGYFGKVGMRHYHLRKNRTFCPS+NIEK+WSLVSDKTY+TYK++ EGKAPVID LKAG FK+LGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC Sbjct: 1 MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSVNIEKIWSLVSDKTYDTYKDHPEGKAPVIDCLKAGIFKVLGKGVLPKVPVIVKAKFFSRQAEEKIKSVGGAC 145 The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L27a vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of 60S ribosomal protein L27a vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of 60S ribosomal protein L27a vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of 60S ribosomal protein L27a vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of 60S ribosomal protein L27a vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 3
BLAST of 60S ribosomal protein L27a vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of 60S ribosomal protein L27a vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 60S ribosomal protein L27a vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 60S ribosomal protein L27a vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of 60S ribosomal protein L27a vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of 60S ribosomal protein L27a vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of 60S ribosomal protein L27a vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of 60S ribosomal protein L27a vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of 60S ribosomal protein L27a vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004808 ID=SMED30004808|Name=60S ribosomal protein L27a|organism=Schmidtea mediterranea sexual|type=transcript|length=447bpback to top protein sequence of SMED30004808-orf-1 >SMED30004808-orf-1 ID=SMED30004808-orf-1|Name=SMED30004808-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MSKIKVKKTRKLRGHVSHGHGRVGKHRKHPGGRGNAGGQHHHRIHMDKFHback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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