SMED30004776
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004776 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30004776 aligns in the following genomic locations:
Homology
BLAST of SMED30004776 vs. Planmine SMEST
Match: SMESG000040928.1 (SMESG000040928.1) HSP 1 Score: 876.7 bits (2264), Expect = 0.000e+0 Identity = 498/502 (99.20%), Postives = 500/502 (99.60%), Query Frame = 2 Query: 107 MVKFYLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKXXXXXXXXXXXXGSQIHLVWHIVDDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAEXXXXXXXXXXXXXMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQFDECSRLRGDSLSHXXXXXXXXXXXXXXXXXXFKIPSFSIILGILFFTMTLILVLIASTLKSSESGKTITVSYAFXXXXXXXXXXXXQMFEMKRWPKKTKIENVVDPALLYLGTSGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESFPLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLIGKYKVQ 1612 MVKFYLFDVIRNWLKLLILFSFTVGIF NYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKILFLFLSIPLFIGSQIHLVWHIV DVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQFDECSRLRGDSLSHENRESIESTNSNISNYYYFKIPSFSIILGILFFTMTLILVLIASTLKSSESGKTITVSYAFSIY+VSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGT+GQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESFPLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLIGKYKVQ Sbjct: 1 MVKFYLFDVIRNWLKLLILFSFTVGIFTNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKILFLFLSIPLFIGSQIHLVWHIVGDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQFDECSRLRGDSLSHENRESIESTNSNISNYYYFKIPSFSIILGILFFTMTLILVLIASTLKSSESGKTITVSYAFSIYIVSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGTAGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESFPLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLIGKYKVQ 502
BLAST of SMED30004776 vs. Planmine SMEST
Match: SMESG000040891.1 (SMESG000040891.1) HSP 1 Score: 219.164 bits (557), Expect = 2.246e-64 Identity = 174/509 (34.18%), Postives = 274/509 (53.83%), Query Frame = 2 Query: 119 YLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKXXXXXXXXXXXXGSQIHLVWHI-VDDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAEXXXXXXXXXXXXXMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKT-----LGDTDTQFDE--CSRLRGDSLSHXXXXXXXXXXXXXXXXXXFKIPSFSIILGILFFTMTLILVLIASTLKSSES----GKTITVSYAFXXXXXXXXXXXXQMFEMKRWPKKTKIENVVDPALLYLGTSGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVF----EIMNNNIVQESF-PLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLI 1594 Y +++ L+L LF F G F YCWAC LD+V+P + FW PIA ++G+++ +L + I + + +LF L PLF+ + + + I V +KL T M + E FIGT L + + F +K+K F++L S IG+ YF+F++ QI +FI +A +Y YS +YNN + + R H ++ Y+QPL I+FC YLI+ FS+T L D T+ E ++ + E SN N F++P S++LGIL T++L+ V I ++L S S G+ ++ + I ++S+ +L +Q+ EM+RWPK K EN+VD L+++G GQI ++ S +LY + ++ +LI+Q LQ L I LSL+R+WI P K+WQE+ICP+ + N+ + +VF +++N + + + LFLK+FGNGF S+ TL ILQR HSS+IF K+I Sbjct: 5 YKYEIFEKLLQLTGLFYFVSGFFTTYCWACKELDKVSPHEQINNFWNPIAVVSGIINCFNFILTIITISQGKRQVQTERNIEGNLLFYVLCAPLFLATIVFTILEITVFSMKLHE-NDLTMLMSIGEMFLFIGTFFLILNINFKFWIFLGSKMKLLNRFVMLTSFIGIINYFMFHMTDQQITNFISIASDYDAYSKYYNNST-KPREGPFHFSRIKKYVQPLVILFCCTCMVYLIKFYFSRTSSQKNLDDNSTELSEETLDKINDSETENLIIRENEVVRSNTKNPPSFRLPPLSLVLGILLITVSLLAVAILNSLLKSTSLYFTGQYLSTIFPIPIDILSLFLLYMQIIEMERWPKIKKNENIVDSTLVWIGAIGQITIAFSQLELYTF-IRDPGLVAAKDCYYFVLILQTILQTLTISLSLDREWISPERKSWQEYICPAVGILSINISFLIINVFILMGDVLNEQMAKNNITELFLKMFGNGFSSLGETLGILQRLHSSLIFIKII 510
BLAST of SMED30004776 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1) HSP 1 Score: 195.667 bits (496), Expect = 1.198e-55 Identity = 181/511 (35.42%), Postives = 290/511 (56.75%), Query Frame = 2 Query: 119 YLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFI-------RVSWNDPTIQVDFYSKXXXXXXXXXXXXGSQIHLVWHIVDDVKL-SSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAEXXXXXXXXXXXXXMENRNLDSHSPD---LEIYLQPLTIIFCFVSFSYL-IRSEFSKTLGDTDTQFDECSRLRGDSLSHXXXXXXXXXXXXXXXXXXFKIPSFSIILGILFFTMTLILVLIASTLKSSE----SGKTITVSYAFXXXXXXXXXXXXQMFEMKRWPKKTKIENVVDPALLYLGTSGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESF-----PLFLKIFGNGFYSISMTLAILQRFHSSIIFYK 1588 Y FD++ L+ L F +G +Y WA LD T + L+FWEPIA I G++++ ++LI +FI + + I + FY LSIPL + S +L+ I+ D ++G F+ F+ E FFI T+ ++ ++ ++ ++ F+ LI+ IGM YF F+IV+ QI +FI VA N S Y + NRN+ + D L Y+QPL I+FC + YL I + + ++ DE +L +S+ ++ S S NSN S ++P S+ILGIL T ++++++I ++ + + SG+ I+ ++A IY++SI++L++Q+FEMKRW K + N+VD L ++G GQ+ ISTA++ + Y+ + S +I+ LIVQI LQ++ + L+L+R+ IPP +KTWQEFICP+ + NLG S+F+++N N Q+SF +F++IFG GF S TL+ILQR HS +IF K Sbjct: 5 YKFDILEKILEFSCLIYFVLGCLTSYSWASKELDYKTLNSRILLFWEPIAAIMGIVNVFHIILIGFFIYKNSKKKIMKKSHKEINILFY------ILSIPLLLVSMSYLILLIIRDFHCQETIGKFSIFLCFGELGFFIATIVFITFINISKLIIPTKQMAQFFRFLKLITIIGMVNYFFFHIVSQQIYNFISVASNTSIYPK------INNRNIPALPFDFDSLRNYMQPLVILFCCICVIYLVIIFRLANANNPSISENDEYIQLI-ESVHNQQNLSQTSKNSNKSFMKNLQLPPISLILGILLITSSILIIIIINSFLNDKLLYKSGQIISATFASFIYLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLM--ISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVINAN-SQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGK 499
BLAST of SMED30004776 vs. Planmine SMEST
Match: SMESG000020517.1 (SMESG000020517.1) HSP 1 Score: 171.785 bits (434), Expect = 8.164e-47 Identity = 151/511 (29.55%), Postives = 249/511 (48.73%), Query Frame = 2 Query: 110 VKFYLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKXXXXXXXXXXXXGSQIHLVWH----IVDDVKLSSVGGFTEFMHVTESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAEXXXXXXXXXXXXXMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQ---FDECSRLRGDSLSHXXXXXXXXXXXXXXXXXXFKIPSFSIILGILFFTMTLILVLIASTLKSSE----SGKTITVSYAFXXXXXXXXXXXXQMFEMKRWPKKTKIENVVDPALLYLGTSGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQ----ESFP-LFLKIFGNGFYSISMTLAILQRFHSSIIFYKLI 1594 +K + D+ LK +L F +G +NY W C LD + L F+ PIA +MD V L+ I W+ + D + L + +V+ I+ ++ S F F + E FI + L + + ++ N + + Y F++L++ G+ +++ QI +FIK+A + S FYN + + +S + +LQP I+FCFV SYLI S +T+ DE + G S S + + + + +P FS+ L ILF T+T ++ IA+ + +S G+ I+ + IY+ SI +L++QM ++ RW K + ++D +L++G +GQ+ ++ + Y Y K S I + IL Q Q LVI L+L+RKW P +++W E++CP + NL + FS+F+I+N + E P LF++IF NGF S + LAILQR HSS+IF K+I Sbjct: 1 MKIFKLDIFEKVLKFYVLLYFVMGFLVNYLWICKELDMKSDFKRILEFYNPIAMATSLMDF--VSLVSVLILFKWSVSKKKTDLEKLESNILLYTIVIPTFLATIVYSSVKVILSAMEFSEKNDFILFSVMGEICLFISVIILIQFFLKSKFIVNYFQ-RIYIDFVVLLNIFGIVNLICYHMSVQQINNFIKIASKFPTLSKFYNLSISQPTDNESSLIKINSFLQPFVILFCFVILSYLILFYNSAHYEIQETEKLLIDEINLRTGASEEDIIASSNPTISFSFKVFQSINLPPFSLGLAILFITVTFFIIAIANNMMNSPKFATEGQLISSIFPIPIYLFSIFLLIIQMNDICRWEKLGTDKYLIDRMILWVGATGQVVEIVAQISMNSYQYYPK-LSGWIIAEYAILFFQTMFQTLVISLALDRKWSQPKLESWHEYLCPPVGMLSLNLSFMIFSLFDIINTKSLNVFSVELNPYLFMRIFTNGFQSFGVALAILQRLHSSLIFGKMI 507
BLAST of SMED30004776 vs. Planmine SMEST
Match: SMESG000040922.1 (SMESG000040922.1) HSP 1 Score: 126.716 bits (317), Expect = 6.216e-31 Identity = 152/514 (29.57%), Postives = 247/514 (48.05%), Query Frame = 2 Query: 107 MVKFYLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWEPIAGINGVMDLSIVLLIVYFIRVSWNDPTIQVDFYSKXXXXXXXXXXXXGSQIHLVWHIVDDVK-LSSVGGFTEFMHVTESVFF---IGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAEXXXXXXXXXXXXXMENRNLDSHS-PDLEIYLQPLTIIFCFVS----FSYLIRSEFSKTLGDTDTQFDECSRLRGDSLSHXXXXXXXXXXXXXXXXXXFKIPSFSIILGILFFTMTLILVLIASTLKSSE---SGKTITVSYAFXXXXXXXXXXXXQMFEMKRWPKKTKIENVVDPALLYLGTSGQIFVSISTAKLYMYNYQNKE-FSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYFGFSVFEIMNNNIVQESFP-----LFLKIFGNGFYSISMTLAILQRFHSSIIFYKLI 1594 M + Y D+ L L F +G NY WAC LD + E FW PIA I G+ ++ I +LI+ + V N +I F + L L +P F+G+ + I+ +K + FT + +E + F IG + + M+ K+ E+ + LI +F+ QI +F+KVA N+SFY FY + NR +D ++ ++ YL P+ I+FC + F L+ ++ TL T+ Q D ++ S ++ N P I+ I + ++I S L S + +G+ I+ + Y ++II+L +Q+ E+ W K + E VD +L++G +GQ+ + AKL+M + E FS L I + +L+VQI +Q + + ++L RKW+ P +K+WQE + P +L N+G+ S+ N N + LFL+IFGNGF + LAI R HSS+IF K+I Sbjct: 1 MNESYWSDLFEQILNFFALSYFILGYLTNYLWACNRLDYTSSYKEIFKFWNPIAVITGISNIIIGILIIIALNVRINRVSIPNHFEYQTLIYTLLVPSFLGTVTFMAIRIILTIKSYMNFNDFTMVLSFSEMILFCCGIGLIRKKISMVNPEVNCYLQKLVEFINKSALIGFCNCLIHFM---SAQQIDNFVKVASNFSFYFQFYGKS-LTNRTVDYYNLSNINSYLVPIAILFCCLCVICLFFNLLNNKSQNTL--TEDQNDSQVIVQWTSTTNLLENEPIFDRVRSLNMS----PLLLILALIFLAISIFLTIIINSFLNSYQYNITGQLISTVFPIPFYFLAIILLAIQINEIMEWKKISTNEFTVDKMMLWIGAAGQLVICF--AKLFMEFIERTENFSALIIAKYGLLLVQIFIQTVALNMALNRKWLRPKLKSWQEKLFPPISLLSLNMGFLFISMLNDFNRNTSHSTVKNIDKRLFLRIFGNGFQLPGLILAIFHRMHSSVIFGKMI 502 The following BLAST results are available for this feature:
BLAST of SMED30004776 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004776 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004776 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004776 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004776 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004776 ID=SMED30004776|Name=SMED30004776|organism=Schmidtea mediterranea sexual|type=transcript|length=1667bpback to top protein sequence of SMED30004776-orf-1 >SMED30004776-orf-1 ID=SMED30004776-orf-1|Name=SMED30004776-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=503bp MVKFYLFDVIRNWLKLLILFSFTVGIFMNYCWACVLLDQVTPINETLIFWback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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