SMED30004749

Overview
NameSMED30004749
Smed IDSMED30004749
Length (bp)817
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004749 (SMED30004749) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004749 (SMED30004749) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004749 (SMED30004749) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004749 (SMED30004749) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004749

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004749SMESG000046853.1 SmedASXL_061260SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30004749SMESG000046853.1 dd_Smed_v4_11984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004749SMESG000046853.1 dd_Smed_v4_11984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30004749SMESG000046853.1 dd_Smed_v4_11984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30004749SMESG000046853.1 dd_Smed_v4_11984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30004749SMESG000046853.1 dd_Smed_v4_11984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30004749SMESG000046853.1 dd_Smed_v6_11984_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30004749 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002824:18657..20053 -3985
Homology
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BLAST of SMED30004749 vs. Planmine SMEST
Match: SMESG000046853.1 (SMESG000046853.1)

HSP 1 Score: 263.077 bits (671), Expect = 2.647e-89
Identity = 156/157 (99.36%), Postives = 156/157 (99.36%), Query Frame = 1 
Query:  112 MSKMEPSASLVDPNLLRSVTNTQFHNIYFHNPIANHSLTPEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTXXXXXXXXXXXXXTSVHGGWNNNRCCLINTLVFNILALXXXXXXXXXXXXXXKYLVDSPSFLYGVYSCC 582
            MSKMEPSASLVDPNLLRSVTNTQFHNIYFHNPIANHSLTPEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTIF GGLMIFIGGITSVHGGWNNNRCCLINTLVFNILALFGFIGGIISFSVSIKYLVDSPSFLYGVYSCC
Sbjct:    1 MSKMEPSASLVDPNLLRSVTNTQFHNIYFHNPIANHSLTPEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTIFVGGLMIFIGGITSVHGGWNNNRCCLINTLVFNILALFGFIGGIISFSVSIKYLVDSPSFLYGVYSCC 157
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BLAST of SMED30004749 vs. Planmine SMEST
Match: SMESG000046804.1 (SMESG000046804.1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.101e-8
Identity = 31/87 (35.63%), Postives = 53/87 (60.92%), Query Frame = 1 
Query:  229 PEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTXXXXXXXXXXXXXTSVHGGWNNNRCCLINTLVFNILAL 489
            P     E      RY+R H+ YG+ ++ +G + + +DP++P +     +++ GGL + I G+ S++G   NN+C LI +L+FNILAL
Sbjct:   61 PPQTTEESQNSMLRYKRMHLIYGIISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILAL 147
[Close]
BLAST of SMED30004749 vs. Planmine SMEST
Match: SMESG000046778.1 (SMESG000046778.1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.492e-8
Identity = 32/80 (40.00%), Postives = 49/80 (61.25%), Query Frame = 1 
Query:  250 DSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTXXXXXXXXXXXXXTSVHGGWNNNRCCLINTLVFNILAL 489
            +S+ T      H R+G+  +F+G LI+  DPL+P     + T  GG + + I GI+S+HG WN++R CLI  +V N LA+
Sbjct:   43 NSKFTLTRPNLHYRFGIIILFLGTLIICFDPLLPFTHTFTNTNSGGTIFMIISGISSIHGSWNHSRGCLITAIVMNSLAV 122
[Close]
BLAST of SMED30004749 vs. Planmine SMEST
Match: SMESG000046815.1 (SMESG000046815.1)

HSP 1 Score: 53.5286 bits (127), Expect = 3.032e-8
Identity = 31/87 (35.63%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  229 PEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTXXXXXXXXXXXXXTSVHGGWNNNRCCLINTLVFNILAL 489
            P     E      RY+R H+ YG  ++ +G + + +DP++P +     +++ GGL + I G+ S++G   NN+C LI +L+FNILAL
Sbjct:   61 PPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILAL 147
[Close]
BLAST of SMED30004749 vs. Planmine SMEST
Match: SMESG000046819.1 (SMESG000046819.1)

HSP 1 Score: 53.5286 bits (127), Expect = 3.332e-8
Identity = 31/87 (35.63%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  229 PEIVISEDSRKTFRYQRHHIRYGVNAMFVGMLIMILDPLMPVLLLSSTTXXXXXXXXXXXXXTSVHGGWNNNRCCLINTLVFNILAL 489
            P     E      RY+R H+ YG  ++ +G + + +DP++P +     +++ GGL + I G+ S++G   NN+C LI +L+FNILAL
Sbjct:   61 PPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILAL 147
The following BLAST results are available for this feature:
BLAST of SMED30004749 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004749 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046853.12.647e-8999.36SMESG000046853.1[more]
SMESG000046804.11.101e-835.63SMESG000046804.1[more]
SMESG000046778.11.492e-840.00SMESG000046778.1[more]
SMESG000046815.13.032e-835.63SMESG000046815.1[more]
SMESG000046819.13.332e-835.63SMESG000046819.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004749 ID=SMED30004749|Name=SMED30004749|organism=Schmidtea mediterranea sexual|type=transcript|length=817bp
TATAATTTAGGGCAAGAAAAATGATTGATTTTTCAAAGTTTTCATTTAAG
TGTTTTTGAATTGTTCACAAACAAATACAACTTTAAGAGAATATCAGAAC
AAGAAATACAAATGAGTAAAATGGAACCTTCCGCTTCATTAGTTGATCCC
AATCTCCTCAGAAGTGTGACTAATACTCAGTTTCACAACATCTATTTTCA
CAATCCTATTGCAAATCATTCGTTGACTCCTGAAATAGTTATTAGTGAAG
ACTCTAGGAAAACCTTTCGTTACCAACGGCATCATATCCGATACGGGGTG
AACGCAATGTTTGTAGGTATGCTTATAATGATACTGGATCCTCTTATGCC
TGTATTACTTCTCTCAAGTACAACAATATTTGGAGGTGGGTTGATGATCT
TTATTGGAGGTATAACGTCGGTTCATGGTGGATGGAATAACAATAGGTGC
TGCTTGATTAATACATTAGTGTTTAATATTTTGGCCTTATTCGGATTCAT
TGGTGGAATCATATCATTTTCCGTATCAATTAAGTATTTAGTTGACAGTC
CGTCTTTTTTATACGGAGTTTACAGTTGTTGCATATCTTCAGTCGAGATT
CTGGTCATATTAATTGACATAATCTTGATCTCAAAGGACATATCACGATA
ATCAGTGACCTCGGGAAACCAATTCTCATCATTGGAAAACTATAATAATT
TACTGTGTTTTATTAGATTTTATTCGCCATTTTTTGTATTAAATTATATT
TACATAAAATGTATTTTATCATTCATTCCAAACCTTTAATAAACATTAAA
TAAATATTTTGAATTGA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0003117parapharyngeal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 87..109
NoneNo IPR availableTMHMMTMhelixcoord: 61..83
NoneNo IPR availableTMHMMTMhelixcoord: 151..173
NoneNo IPR availableTMHMMTMhelixcoord: 114..136