J domain-containing protein

Overview
NameJ domain-containing protein
Smed IDSMED30004737
Length (bp)1089
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of J domain-containing protein (SMED30004737) t-SNE clustered cells

Violin plots show distribution of expression levels for J domain-containing protein (SMED30004737) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of J domain-containing protein (SMED30004737) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for J domain-containing protein (SMED30004737) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30004737

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30004737h1SMcG0020324 dd_Smed_v4_9458_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004737h1SMcG0020324 dd_Smed_v4_9458_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of J domain-containing protein vs. Ensembl Fly
Match: wus (gene:FBgn0030805 transcript:FBtr0074418)

HSP 1 Score: 63.929 bits (154), Expect = 6.511e-11
Identity = 75/336 (22.32%), Postives = 138/336 (41.07%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE-NEMYYSDNFLRT-------------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLAN------KYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIE-GKSTKIRYVLTSDSFLQFLVQLRAIITNSY---RQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS*YQFSEYQ 1037
            R RH F+W  T GG+   GW  ++F I  Y R+ NE      F++              SS R +G++++G  F  +  + +PE        S      +P  V++G+ LV   GR   +  W   + +YL     +   D  T+S++ T  +A           RR P   ++ + G  +       I     W  FL  N  I+ E G    I   + +     +   L+  + ++Y   + +G + T+   ++++ D      + + L + + AS   I    R LS ++HPD+  D+ ++    + +  I+     L K+      +  +YQ
Sbjct:   73 RDRHAFIWWCTLGGYMGIGWMGELFLIPEYVRDANE---DPKFVKMFVAKLQAYPKPPYSSKRFVGQVMIGHLFGQVCSMAIPETLVGGWDLSFL-HWTIPLFVSLGVWLVGNIGREQGV-WWHCLVAAYLAYPARYLIYDE-TYSLLLTGLVAALTFDGLSKQWRRTPP--RRGSPGERTFKLTTAVIIYCAFWGSFLYFNGTISDEDGGEVPIHEAIHNFLASAWWTDLKQALLDTYNYAQHHGWYETWKEVFESM-DVDGERNSYKVLGVSATASQAEITAAYRKLSKEYHPDKVKDEGLRAQAHQRFIEIQQAYSVLSKIKSNRRRKNKQYQ 399
[Close]
BLAST of J domain-containing protein vs. Ensembl Fly
Match: wus (gene:FBgn0030805 transcript:FBtr0342974)

HSP 1 Score: 63.929 bits (154), Expect = 6.511e-11
Identity = 75/336 (22.32%), Postives = 138/336 (41.07%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE-NEMYYSDNFLRT-------------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLAN------KYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIE-GKSTKIRYVLTSDSFLQFLVQLRAIITNSY---RQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS*YQFSEYQ 1037
            R RH F+W  T GG+   GW  ++F I  Y R+ NE      F++              SS R +G++++G  F  +  + +PE        S      +P  V++G+ LV   GR   +  W   + +YL     +   D  T+S++ T  +A           RR P   ++ + G  +       I     W  FL  N  I+ E G    I   + +     +   L+  + ++Y   + +G + T+   ++++ D      + + L + + AS   I    R LS ++HPD+  D+ ++    + +  I+     L K+      +  +YQ
Sbjct:   73 RDRHAFIWWCTLGGYMGIGWMGELFLIPEYVRDANE---DPKFVKMFVAKLQAYPKPPYSSKRFVGQVMIGHLFGQVCSMAIPETLVGGWDLSFL-HWTIPLFVSLGVWLVGNIGREQGV-WWHCLVAAYLAYPARYLIYDE-TYSLLLTGLVAALTFDGLSKQWRRTPP--RRGSPGERTFKLTTAVIIYCAFWGSFLYFNGTISDEDGGEVPIHEAIHNFLASAWWTDLKQALLDTYNYAQHHGWYETWKEVFESM-DVDGERNSYKVLGVSATASQAEITAAYRKLSKEYHPDKVKDEGLRAQAHQRFIEIQQAYSVLSKIKSNRRRKNKQYQ 399
[Close]
BLAST of J domain-containing protein vs. Ensembl Zebrafish
Match: dnajc22 (DnaJ (Hsp40) homolog, subfamily C, member 22 [Source:ZFIN;Acc:ZDB-GENE-090313-196])

HSP 1 Score: 56.225 bits (134), Expect = 1.786e-8
Identity = 41/128 (32.03%), Postives = 59/128 (46.09%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCISRYTRENEMYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASK-----LVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFA 473
            R  H  LW++TFGGF  GW R+ F I  Y  E               R +G++ +G+YF              + L SL+S      LVLP  VA G+ LV   G+  S ++ KT +   +TS I++ 
Sbjct:   27 RDSHALLWILTFGGFGIGWAREFFRIPSYVSEANRDVEKGL---GLIRFVGQICVGVYFG------------SVALISLSSLSFFYFLVLPLSVAAGVHLVSSVGQQTS-NLHKTLITCVITSSIFYG 138
[Close]
BLAST of J domain-containing protein vs. Ensembl Zebrafish
Match: dnajc22 (DnaJ (Hsp40) homolog, subfamily C, member 22 [Source:ZFIN;Acc:ZDB-GENE-090313-196])

HSP 1 Score: 56.225 bits (134), Expect = 2.113e-8
Identity = 42/137 (30.66%), Postives = 61/137 (44.53%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCISRYTRENEMYYSDNFLRTSS---------FRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASK-----LVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFA 473
            R  H  LW++TFGGF  GW R+ F I  Y  E         +R             R +G++ +G+YF              + L SL+S      LVLP  VA G+ LV   G+  S ++ KT +   +TS I++ 
Sbjct:   27 RDSHALLWILTFGGFGIGWAREFFRIPSYVSEANRDVEKGRVRHPGATPPPPVGLIRFVGQICVGVYF------------GSVALISLSSLSFFYFLVLPLSVAAGVHLVSSVGQQTS-NLHKTLITCVITSSIFYG 150
[Close]
BLAST of J domain-containing protein vs. Ensembl Zebrafish
Match: dnajc22 (DnaJ (Hsp40) homolog, subfamily C, member 22 [Source:ZFIN;Acc:ZDB-GENE-090313-196])

HSP 1 Score: 56.225 bits (134), Expect = 2.200e-8
Identity = 42/137 (30.66%), Postives = 61/137 (44.53%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCISRYTRENEMYYSDNFLRTSS---------FRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASK-----LVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFA 473
            R  H  LW++TFGGF  GW R+ F I  Y  E         +R             R +G++ +G+YF              + L SL+S      LVLP  VA G+ LV   G+  S ++ KT +   +TS I++ 
Sbjct:   27 RDSHALLWILTFGGFGIGWAREFFRIPSYVSEANRDVEKGRVRHPGATPPPPVGLIRFVGQICVGVYF------------GSVALISLSSLSFFYFLVLPLSVAAGVHLVSSVGQQTS-NLHKTLITCVITSSIFYG 150
[Close]
BLAST of J domain-containing protein vs. Ensembl Xenopus
Match: mcm2 (minichromosome maintenance complex component 2 [Source:Xenbase;Acc:XB-GENE-999974])

HSP 1 Score: 56.225 bits (134), Expect = 3.159e-8
Identity = 74/325 (22.77%), Postives = 135/325 (41.54%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCIS----RYTREN--EMYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITN----SYRQNGVF---GTFT--YF--WDNIRD------PHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKT 1010
            R  H  LW++T GGF  GW  D + I     +Y R+    +   +     S  R +G++  GIYF ++  + L          S    +VLP  VA+G+ LV   G   S  +  T + ++LTS I++ +   +   +  T  + ++  +R      K++ +        ++ +A      +  +LN  + +   +  I  +L    +L     + A++       YR   VF   G F   YF  W+ I +          E A + L +   ++ME I +  R L    HPD    ++ +    E +  I+   + L++L K+
Sbjct:   27 RDSHALLWMLTLGGFGMGWMWDFWKIPIHVYKYNRQERKNIEVKEGEPPASPIRFIGQVATGIYFGIVAAIGL-------SFLSSFHMVVLPLAVALGVHLVATVGEQTS-DLKNTLIAAFLTSPIFYGRAVSM-IPISLTASITSQKHMRYRLQQEKQEKLSLRLYRIGLVYLAFTGPLAYSALLNTSLTVSYVAGSIGSML---EWLSIFPSISALVERLLLLPYRVWAVFSGGGVFRDHYFKEWEKIYEFVATFQSEKEEMACKVLGVNFKSTMEEINRKYRELVKIWHPDHNRHRLEEA--QEHFLEIQAAYETLMRLRKS 337
[Close]
BLAST of J domain-containing protein vs. UniProt/SwissProt
Match: sp|A9ULE9|DJC22_XENTR (DnaJ homolog subfamily C member 22 OS=Xenopus tropicalis OX=8364 GN=dnajc22 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.674e-7
Identity = 74/325 (22.77%), Postives = 135/325 (41.54%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCIS----RYTREN--EMYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITN----SYRQNGVF---GTFT--YF--WDNIRD------PHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKT 1010
            R  H  LW++T GGF  GW  D + I     +Y R+    +   +     S  R +G++  GIYF ++  + L          S    +VLP  VA+G+ LV   G   S  +  T + ++LTS I++ +   +   +  T  + ++  +R      K++ +        ++ +A      +  +LN  + +   +  I  +L    +L     + A++       YR   VF   G F   YF  W+ I +          E A + L +   ++ME I +  R L    HPD    ++ +    E +  I+   + L++L K+
Sbjct:   27 RDSHALLWMLTLGGFGMGWMWDFWKIPIHVYKYNRQERKNIEVKEGEPPASPIRFIGQVATGIYFGIVAAIGL-------SFLSSFHMVVLPLAVALGVHLVATVGEQTS-DLKNTLIAAFLTSPIFYGRAVSM-IPISLTASITSQKHMRYRLQQEKQEKLSLRLYRIGLVYLAFTGPLAYSALLNTSLTVSYVAGSIGSML---EWLSIFPSISALVERLLLLPYRVWAVFSGGGVFRDHYFKEWEKIYEFVATFQSEKEEMACKVLGVNFKSTMEEINRKYRELVKIWHPDHNRHRLEEA--QEHFLEIQAAYETLMRLRKS 337
[Close]
BLAST of J domain-containing protein vs. TrEMBL
Match: A0A1B6FPN6 (J domain-containing protein OS=Cuerna arida OX=1464854 GN=g.16821 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.350e-18
Identity = 83/317 (26.18%), Postives = 136/317 (42.90%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE--NEMYYSDNFLRT---------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLAS-KLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFS--VIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEI-NIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSY---RQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLK 998
            R    FLW  T GG+F FGW RDIF I  Y  +   +  + + F+ T         SS R  G ++MG ++  +  + +PE    +  +SL    + +P  VA+G+  V   G     S+W   + SY+    Y+      +F+  VIFT F+ +  +      +  +K+        LI G+     W  +   N ++ N EG+   +   +       +    +  + N++   R NG   T+    D + DP     AL+ L L S A+   I  TCR L  K HPD+      K    E++  ++  C+ L K
Sbjct:   28 RDIQAFLWWCTLGGYFGFGWLRDIFYIPSYVADANQDPDFVNKFVTTLRTNPKPPFSSVRFCGMVIMGYFWGSVVCMSIPEE--GVAGYSLQWLHVFIPVAVALGVWAVGNIGN-EEGSLWWPLVASYIVYPFYWHYNSDTSFTALVIFTAFVFDTKAKHWRTRVRPRKSFARRLTVLLICGLLYFSLWGSYFYFNAKLQNSEGEEVPVHEAIRHFFHSPWWADFKTSLDNTWQFARHNGWLATWRLIVD-LGDPFGETHALQVLNLSSSANQTEINTTCRMLKVKFHPDKAKTPADKQTAQEKFYEVQQACEILSK 340
[Close]
BLAST of J domain-containing protein vs. TrEMBL
Match: A0A2A4JJX6 (J domain-containing protein OS=Heliothis virescens OX=7102 GN=B5V51_1186 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.900e-17
Identity = 85/315 (26.98%), Postives = 139/315 (44.13%), Query Frame = 3 
Query:  102 KRPRHGFLWLITFGGFFFGWFRDIFCISRYTR---ENEMYYSDNFLRT--------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLT-SVIYFAKEDYITFSVIF----TCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINI---CWIFFLVLNLEI-NIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNI---RDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRM 977
            +R RH F+W  T GGFF GW  +IF I RY R   E+  +  +   R         S  R  G L++G  +  +    +P +++ + L  L   L++P  VA+G+  V   GR      W   + +YL   V Y+  +D + F+V+       F +     RR P   KKKT   + +  +ILG+   +    W  +L  +  I + EG    I   L       + + ++  +  +Y+     G +   W  +    DPH  + A + L +  DAS + I  T R LS +HHPD+  D+  +    E +  I+ 
Sbjct:   35 RRDRHAFVWWTTLGGFFVGWLGEIFRIPRYVRDANEDPKHMEELANRMIQNKKPPFSMNRFTGMLMVGYSWGQMTMFAIPPDFWGMNLKYL--NLLIPLSVALGVWTVGNIGREQGSLKW-PLIGAYLGYPVRYYVYDDSLWFTVMLLASACAFDSYSKEWRRTP---KKKTHIAKRV--VILGLCATLYLGLWCSYLYFHGTITDSEGDEVPIYEALHHFFTSPWWLDVKQCLLETYQYAQHHGWYE-VWKQVIDMSDPHGEQNAYKVLGVDRDASQQEITSTWRKLSREHHPDKVKDEKERRAAQERFMEIQQ 340
[Close]
BLAST of J domain-containing protein vs. TrEMBL
Match: A0A1B6MKZ6 (J domain-containing protein OS=Graphocephala atropunctata OX=36148 GN=g.33432 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.919e-16
Identity = 84/317 (26.50%), Postives = 138/317 (43.53%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE--NEMYYSDNF---LRT------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLAS-KLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFS--VIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEI-NIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSY---RQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLK 998
            R    FLW  T GG+F FGW RDIF I  Y  +   +  + + F   LRT      SS R  G ++MG ++  +  + +PE    +  ++L    + +P  VA+G+  V   G     S+W   + SYL    Y+      +F+  VIFT F+ +  +      I  +K+        LI G+     W  +   N ++ + +G+   +   +       +    ++ + N++   R NG   T+    D + DP     AL+ L L S A+   I  TCR L  K HPD+      K    E++  ++  C+ L K
Sbjct:   28 RDIQAFLWWCTLGGYFGFGWLRDIFYIPSYVADANQDPEFVNKFVSSLRTNPKPPFSSVRFCGMVIMGYFWGSVVYMSIPEE--GVAGYNLQWLHVFIPLAVALGVWAVGNIGN-EEGSLWWPLVSSYLVYPFYWHYNSDTSFTALVIFTAFVFDSKAKHWRTRIRPRKSFVRRLTVLLICGLLYFSLWGSYFYFNAKLQDSDGEEVPVHEAIRHFFRSPWWADFKSSLDNTWQFARHNGWLATWRLIVD-LGDPFGETHALQVLNLSSSANQTEINTTCRMLKVKFHPDKAKTAADKQTVQEKFYEVQQACEILSK 340
[Close]
BLAST of J domain-containing protein vs. TrEMBL
Match: A0A1B6M2P0 (J domain-containing protein OS=Graphocephala atropunctata OX=36148 GN=g.33434 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 3.021e-16
Identity = 83/317 (26.18%), Postives = 138/317 (43.53%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE--NEMYYSDNF---LRT------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLAS-KLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFS--VIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEI-NIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSY---RQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLK 998
            R    FLW  T GG+F FGW RDIF I  Y  +   +  + + F   LRT      SS R  G ++MG ++  +  + +PE    +  ++L    + +P  VA+G+  V   G     S+W   + SYL    Y+      +F+  VIFT F+ +  +      +  +K+        LI G+     W  +   N ++ + +G+   +   +       +    ++ + N++   R NG   T+    D + DP     AL+ L L S A+   I  TCR L  K HPD+      K    E++  ++  C+ L K
Sbjct:   28 RDIQAFLWWCTLGGYFGFGWLRDIFYIPSYVADANQDPEFVNKFVSSLRTNPKPPFSSVRFCGMVIMGYFWGSVVYMSIPEE--GVAGYNLQWLHVFIPLAVALGVWAVGNIGN-EEGSLWWPLVSSYLVYPFYWHYNSDTSFTALVIFTAFVFDSKAKHWRTRVRPRKSFVRRLTVLLICGLLYFSLWGSYFYFNAKLQDSDGEEVPVHEAIRHFFRSPWWADFKSSLDNTWQFARHNGWLATWRLIVD-LGDPFGETHALQVLNLSSSANQTEINTTCRMLKVKFHPDKAKTAADKQTVQEKFYEVQQACEILSK 340
[Close]
BLAST of J domain-containing protein vs. TrEMBL
Match: A0A2H1VN18 (SFRICE000237.2 OS=Spodoptera frugiperda OX=7108 GN=SFRICE000237.2 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.640e-15
Identity = 82/313 (26.20%), Postives = 129/313 (41.21%), Query Frame = 3 
Query:  102 KRPRHGFLWLITFGGFFFGWFRDIFCISRYTRE--NEMYYSDNFLRT---------SSFRHLGELVMGI-YFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIF----TCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINIC-WIFFLVLNLEI-NIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNI---RDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRM 977
            +R RH F+W  T GGF  GW  +IF I RY RE  N+  Y +   R          S  R  G L++G  +  M    I P++++ I L  L   L++P  +A+G+  V   G       W          + Y+  +D I F+++       F +     RR P   KKKT   + +  L     I +  W  +L  +  I + EG    I   L       + + ++  I  +Y+     G +   W  I    DPH  + A   L +  D+S   I    R LS ++HPD+  D+  +    E +  I+ 
Sbjct:   33 RRDRHAFVWWTTLGGFVIGWLGEIFRIPRYVREANNDPQYVEELARRVIQNKKPPFSMNRFTGMLMVGYSWAQMTMFAIPPDSFWGINLRYL--NLLIPVALALGVWTVGNIGNEEGSLKWPLLGAFAAYPIRYYVFDDSIWFTIMLLASACAFDSYSKRWRRTP---KKKTHNVKRVSILAACACIYLSLWCSYLYFHGTITDSEGDEVPIYEALHHFFTSPWWLDVKQCIAETYQYAQHHGWYE-VWKQIIDLSDPHGEQNAYRVLGVNRDSSQSEITSVWRKLSRENHPDKVKDEAERRAAQERFMEIQQ 339
[Close]
BLAST of J domain-containing protein vs. Ensembl Cavefish
Match: dnajc22 (DnaJ heat shock protein family (Hsp40) member C22 [Source:NCBI gene;Acc:103040633])

HSP 1 Score: 54.6842 bits (130), Expect = 4.913e-8
Identity = 40/131 (30.53%), Postives = 60/131 (45.80%), Query Frame = 3 
Query:  102 KRPRHGFLWLITFGGFFFGWFRDIFCISRYTRENEMYYSDNFLRTSS-------FRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFA 473
            +R  H  LW++T GGF  GW R+ F +  Y  E +    +   R+S+       FR  G++ +GIYF  +           +Q  S    +VLP  V  GI LV   G+  S  + KT     +TS I++ 
Sbjct:   25 RRDSHALLWMLTLGGFGVGWMREFFRLPAYVSEAKK-KPEEVKRSSTTAPPVGLFRFAGQICVGIYFGSV-------ALISLQSLSFFYLIVLPLSVGAGIHLVSCVGQQTS-DLQKTLTTCIITSSIFYG 146
[Close]
BLAST of J domain-containing protein vs. Ensembl Cavefish
Match: dnajc22 (DnaJ heat shock protein family (Hsp40) member C22 [Source:NCBI gene;Acc:103040633])

HSP 1 Score: 54.6842 bits (130), Expect = 4.913e-8
Identity = 40/131 (30.53%), Postives = 60/131 (45.80%), Query Frame = 3 
Query:  102 KRPRHGFLWLITFGGFFFGWFRDIFCISRYTRENEMYYSDNFLRTSS-------FRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFA 473
            +R  H  LW++T GGF  GW R+ F +  Y  E +    +   R+S+       FR  G++ +GIYF  +           +Q  S    +VLP  V  GI LV   G+  S  + KT     +TS I++ 
Sbjct:   25 RRDSHALLWMLTLGGFGVGWMREFFRLPAYVSEAKK-KPEEVKRSSTTAPPVGLFRFAGQICVGIYFGSV-------ALISLQSLSFFYLIVLPLSVGAGIHLVSCVGQQTS-DLQKTLTTCIITSSIFYG 146
[Close]
BLAST of J domain-containing protein vs. Ensembl Nematostella
Match: EDO43846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXH6])

HSP 1 Score: 70.8626 bits (172), Expect = 9.586e-14
Identity = 76/293 (25.94%), Postives = 119/293 (40.61%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFF-FGWFRDIFCISRYTRE--NEMYYSDNFLRT---------SSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINIC--WIFFLVLNLEINIE-GKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNIR--DPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQ 932
            R    F+W  TFGG F  GW RD++ I  Y  +   + YY +   R          S+ R  G++++G ++ +L RL +PE+            L +P  VA GI LV   GR      +K  LI  L S        Y + + I                       G   +  L LG  I +C  W  F+  N E+  E G++ K+R  +         ++ + ++   Y +    G    + D ++  DP   + +   L L  DA+ E I+K  + L+ K HPDR  D 
Sbjct:   30 RDIQAFVWWSTFGGVFGLGWLRDLWRIPEYVEDANEDHYYIEELKRKIKLRKEPPFSTTRFAGQMLVGYFYGILTRLAIPES-----APKWTPALWVPLAVASGIYLVGNIGR--ERGDFKYALIGCLVS--------YASITFI------------------NGDEAGNIRVQVLALGGLI-MCALWGSFIYFNAEVTTEDGETIKLRDAVNHFFNSPVWLEFKDVLWQLYDEGQKNGWQNLYDDFVKALDPRGEKNSYRVLGLTEDATQEEIKKRYKKLAMKWHPDRHRDN 288
[Close]
BLAST of J domain-containing protein vs. Ensembl Medaka
Match: dnajc22 (DnaJ heat shock protein family (Hsp40) member C22 [Source:NCBI gene;Acc:101169606])

HSP 1 Score: 59.3066 bits (142), Expect = 1.955e-9
Identity = 81/329 (24.62%), Postives = 131/329 (39.82%), Query Frame = 3 
Query:  105 RPRHGFLWLITFGGFFFGWFRDIFCISRYTR------ENEMYYSDN--FLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKK-KTIGYE----SICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQ-LRAIITNSYRQNGVFGTFTY---FWDNIRD------PHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS*YQ 1022
            R  H  LW++T GGF FGW R++  I  Y R      E E    +N         R  G++ +G+YF  +  + L      +  F L   +VLP  V  G+ LV   G   S  + KT     + S +++          +     A+++   + P  T   + +G      ++ +L     I  C IF+   N    +   S  I  +L    FL +L   L  I+   YR         Y    W  I +          ++AL+   L SDAS+E I ++ R L+   HPD    Q  + +    +  ++   + LL+ HK   +Q
Sbjct:   27 RDSHALLWMLTLGGFGFGWIREVIRIPAYVRAANKDPEKERRKENNRVPPPPGPVRFAGQVCVGMYFGAVALIGL----NSLSFFYL---IVLPLSVGAGVHLVSSVGEQTS-DLKKTLTACLMMSPVFYGSTLSPLPISLAASITASQHRRFKPPQTTSSTQKLGPRLYKLALAWLAFSAPIGYC-IFY---NTTATLYYISDSIAALLDIFWFLPWLRGLLEYILLIPYRILCALTGGGYQEEAWRKILEILLKEYTEKEKQALQVFSLKSDASLEEITRSYRELAKTWHPDHNPSQEAEAM----FLKVQEAYEVLLRWHKPQRFQ 339
[Close]
BLAST of J domain-containing protein vs. Planmine SMEST
Match: SMESG000043491.1 (SMESG000043491.1)

HSP 1 Score: 548.51 bits (1412), Expect = 0.000e+0
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 3 
Query:  207 MYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS 1013
            MYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS
Sbjct:    1 MYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQLFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKEDYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWIFFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGTFTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLDQVMKNLHAEEYSVIRMHCDNLLKLHKTS 269
The following BLAST results are available for this feature:
BLAST of J domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of J domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of J domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
wus6.511e-1122.32gene:FBgn0030805 transcript:FBtr0074418[more]
wus6.511e-1122.32gene:FBgn0030805 transcript:FBtr0342974[more]
back to top
BLAST of J domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dnajc221.786e-832.03DnaJ (Hsp40) homolog, subfamily C, member 22 [Sour... [more]
dnajc222.113e-830.66DnaJ (Hsp40) homolog, subfamily C, member 22 [Sour... [more]
dnajc222.200e-830.66DnaJ (Hsp40) homolog, subfamily C, member 22 [Sour... [more]
back to top
BLAST of J domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mcm23.159e-822.77minichromosome maintenance complex component 2 [So... [more]
back to top
BLAST of J domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of J domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|A9ULE9|DJC22_XENTR1.674e-722.77DnaJ homolog subfamily C member 22 OS=Xenopus trop... [more]
back to top
BLAST of J domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B6FPN61.350e-1826.18J domain-containing protein OS=Cuerna arida OX=146... [more]
A0A2A4JJX63.900e-1726.98J domain-containing protein OS=Heliothis virescens... [more]
A0A1B6MKZ61.919e-1626.50J domain-containing protein OS=Graphocephala atrop... [more]
A0A1B6M2P03.021e-1626.18J domain-containing protein OS=Graphocephala atrop... [more]
A0A2H1VN181.640e-1526.20SFRICE000237.2 OS=Spodoptera frugiperda OX=7108 GN... [more]
back to top
BLAST of J domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnajc224.913e-830.53DnaJ heat shock protein family (Hsp40) member C22 ... [more]
dnajc224.913e-830.53DnaJ heat shock protein family (Hsp40) member C22 ... [more]
back to top
BLAST of J domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of J domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of J domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO438469.586e-1425.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of J domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dnajc221.955e-924.62DnaJ heat shock protein family (Hsp40) member C22 ... [more]
back to top
BLAST of J domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000043491.10.000e+0100.00SMESG000043491.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004737 ID=SMED30004737|Name=J domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1089bp
ATCTATATAAATCTTATATGAAAGAGTAAATGAGTAAAATTATTTTTGGA
TATATTATTTGGTTGTTATTTGGTCTATTTGGTTTACATCATTTTTATTT
AAAACGACCAAGACATGGTTTTCTATGGCTTATTACGTTTGGTGGATTCT
TTTTTGGTTGGTTTAGAGATATATTTTGTATATCTCGATACACCAGAGAG
AATGAAATGTATTATAGTGATAATTTTCTAAGAACAAGTTCATTTCGACA
TCTTGGTGAATTAGTGATGGGAATTTATTTCCCAATGCTTTTTCGATTAA
TTTTACCAGAAAACTATTTTGAAATTCAATTATTTTCTTTGGCTTCAAAG
TTGGTTCTTCCGTCACTAGTAGCAATCGGAATATTGTTAGTGGATGGAGC
AGGTCGGTTTCTCTCTCTGAGCATGTGGAAAACTTTCCTTATTTCCTATC
TCACATCAGTTATTTATTTTGCCAAGGAGGATTATATAACATTTTCAGTC
ATTTTCACCTGCTTCCTAGCCAACAAATATAGCCTTCGCCGACTTCCATC
TATTACCAAAAAAAAGACTATTGGTTATGAATCAATCTGCTATCTGATTT
TGGGGATCGCAATAAATATTTGTTGGATCTTTTTCCTTGTTTTGAACCTC
GAAATCAATATCGAAGGAAAATCCACGAAAATTCGATATGTTTTGACTTC
GGATTCCTTTCTGCAATTCTTGGTTCAATTGCGCGCGATAATTACTAACA
GCTATCGTCAGAATGGTGTCTTTGGTACATTCACATATTTCTGGGACAAT
ATTCGCGATCCTCATCATTCCGAAAAGGCCTTAGAATATTTGAAACTACC
GTCAGATGCTTCCATGGAACTGATCCGAAAAACATGTCGTGCTTTGAGCT
CCAAGCATCATCCTGACAGATTTCTTGATCAAGTCATGAAGAATTTGCAT
GCTGAAGAATACAGTGTGATACGGATGCATTGTGATAATTTACTGAAGCT
CCATAAGACTTCATAGTATCAATTCTCTGAATATCAGTCGTTTTGGGTTT
TGGGCTCAATTTTATTGTAATTAAATATATTTGATCATA
back to top

protein sequence of SMED30004737-orf-1

>SMED30004737-orf-1 ID=SMED30004737-orf-1|Name=SMED30004737-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=329bp
MSKIIFGYIIWLLFGLFGLHHFYLKRPRHGFLWLITFGGFFFGWFRDIFC
ISRYTRENEMYYSDNFLRTSSFRHLGELVMGIYFPMLFRLILPENYFEIQ
LFSLASKLVLPSLVAIGILLVDGAGRFLSLSMWKTFLISYLTSVIYFAKE
DYITFSVIFTCFLANKYSLRRLPSITKKKTIGYESICYLILGIAINICWI
FFLVLNLEINIEGKSTKIRYVLTSDSFLQFLVQLRAIITNSYRQNGVFGT
FTYFWDNIRDPHHSEKALEYLKLPSDASMELIRKTCRALSSKHHPDRFLD
QVMKNLHAEEYSVIRMHCDNLLKLHKTS*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
Vocabulary: INTERPRO
TermDefinition
IPR036869J_dom_sf
IPR001623DnaJ_domain
IPR007829TM2
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007829TM2 domainPFAMPF05154TM2coord: 4..49
e-value: 1.2E-10
score: 41.5
NoneNo IPR availablePANTHERPTHR44733FAMILY NOT NAMEDcoord: 3..301
NoneNo IPR availableTMHMMTMhelixcoord: 71..93
NoneNo IPR availableTMHMMTMhelixcoord: 29..51
NoneNo IPR availableTMHMMTMhelixcoord: 108..130
NoneNo IPR availableTMHMMTMhelixcoord: 5..24
NoneNo IPR availableTMHMMTMhelixcoord: 185..207
NoneNo IPR availableTMHMMTMhelixcoord: 137..159
IPR001623DnaJ domainCDDcd06257DnaJcoord: 269..301
e-value: 2.22764E-4
score: 36.7545
IPR036869Chaperone J-domain superfamilySUPERFAMILYSSF46565Chaperone J-domaincoord: 233..311