SMED30004730

Overview
NameSMED30004730
Smed IDSMED30004730
Length (bp)1034
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004730 (SMED30004730) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004730 (SMED30004730) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004730 (SMED30004730) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004730 (SMED30004730) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30004730

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30004730SMESG000047424.1 SMESG000029259.1 Contig14663newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004730SMESG000047424.1 SMESG000029259.1 Contig14663uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30004730 vs. Ensembl Zebrafish
Match: si:dkey-206p8.1 (si:dkey-206p8.1 [Source:ZFIN;Acc:ZDB-GENE-131127-460])

HSP 1 Score: 50.8322 bits (120), Expect = 6.098e-7
Identity = 29/78 (37.18%), Postives = 46/78 (58.97%), Query Frame = 2 
Query:  119 REILFSKAEKLKENGLTEDSAFQCY-KCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDE 349
            RE LF    +L+++G ++ +A  C+  CL  LT  + F SLP CL  +   + +++NY  A+Q I A K +Y+ AL E
Sbjct:    6 REGLFELGRQLQQDGDSQ-AALHCFLSCLLGLTHVQSFHSLPNCLHQIAELYIDEKNYGKALQFIQAEKMFYEVALIE 82
[Close]
BLAST of SMED30004730 vs. TrEMBL
Match: B3SAF1 (Predicted protein OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_61237 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.983e-8
Identity = 33/81 (40.74%), Postives = 48/81 (59.26%), Query Frame = 2 
Query:  104 LSKEEREILFSKAEKLKENGLTEDSAFQCY-KCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSAL 343
            L  EERE LF K  + K+ G   D A +C+  CL+ +  N  F  LP+CL  +   +FE++N+  A+Q I A K YY++AL
Sbjct:  129 LRAEEREELFQKGTRFKDKG-NSDLALRCFMACLKGINNNNAFSLLPQCLHSIATIYFERENFDKAVQFIQAEKLYYETAL 208
[Close]
BLAST of SMED30004730 vs. Planmine SMEST
Match: SMESG000047424.1 (SMESG000047424.1)

HSP 1 Score: 478.789 bits (1231), Expect = 2.366e-172
Identity = 236/236 (100.00%), Postives = 236/236 (100.00%), Query Frame = 2 
Query:   50 MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNELDFDFRYLTSIFSTKFEENEERAEILNQAVSVQSSPDVCMLINNLTAIGDTENLSTVVQEQSSYLDITRDSNMESNVTIENQGDNP 757
            MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNELDFDFRYLTSIFSTKFEENEERAEILNQAVSVQSSPDVCMLINNLTAIGDTENLSTVVQEQSSYLDITRDSNMESNVTIENQGDNP
Sbjct:    1 MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNELDFDFRYLTSIFSTKFEENEERAEILNQAVSVQSSPDVCMLINNLTAIGDTENLSTVVQEQSSYLDITRDSNMESNVTIENQGDNP 236
[Close]
BLAST of SMED30004730 vs. Planmine SMEST
Match: SMESG000047424.1 (SMESG000047424.1)

HSP 1 Score: 303.138 bits (775), Expect = 2.305e-104
Identity = 149/151 (98.68%), Postives = 150/151 (99.34%), Query Frame = 2 
Query:   50 MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNEL 502
            MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHN+ 
Sbjct:    1 MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNDF 151
[Close]
BLAST of SMED30004730 vs. Planmine SMEST
Match: SMESG000019784.1 (SMESG000019784.1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.003e-14
Identity = 38/103 (36.89%), Postives = 64/103 (62.14%), Query Frame = 2 
Query:  104 LSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEYENK 412
            L+K ERE LF +AEK   +G    +      CL++L  +  FPSLP CL+ L+ ++ E+++YM +I  + A + YY+SAL EAA++  K+++ +  +   E+K
Sbjct:   17 LNKSERENLFHEAEKFYNSGDRAKAVSLFMNCLQSLDTHSAFPSLPMCLRDLSSHYLEQKDYMKSIHFVQAERMYYESALVEAAMLQEKLIDLAYHHKSPESK 119
[Close]
BLAST of SMED30004730 vs. Planmine SMEST
Match: SMESG000022965.1 (SMESG000022965.1)

HSP 1 Score: 67.781 bits (164), Expect = 1.469e-12
Identity = 42/100 (42.00%), Postives = 56/100 (56.00%), Query Frame = 2 
Query:  104 LSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLNDEY 403
            L+K ERE LF +AE L   G  E S     +CL+NL  N  F SLPKCL+ L  YF  K+ Y  ++  I A + YY+SAL + +    K+ E S   D+Y
Sbjct:    8 LTKFEREELFQEAEILNAEGHEEASIALYLRCLQNLEINSTFGSLPKCLRALANYFLRKKEYDKSVHFIQAERLYYESALLDTSSFQKKLNELS--GDKY 105
[Close]
BLAST of SMED30004730 vs. Planmine SMEST
Match: SMESG000057093.1 (SMESG000057093.1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.198e-7
Identity = 29/98 (29.59%), Postives = 57/98 (58.16%), Query Frame = 2 
Query:  104 LSKEEREILFSKAEKLKENGLTEDSAFQCYKCLRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDEAALIHSKIVEYSQLND 397
            ++  EREI F KAEK ++ GL + S     +CL++L  +  F ++PKCL+ L  +++ K     ++  + A + YY + L E  ++ +K+ + S+ ++
Sbjct:    1 MTTSEREINFQKAEKYQKIGLLDKSVVYYRRCLQDLEEDTEFSNIPKCLRSLANFYWIKGKTKKSLSFLEAEELYYSNILKEIKILQNKLSKKSKFSN 98
The following BLAST results are available for this feature:
BLAST of SMED30004730 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkey-206p8.16.098e-737.18si:dkey-206p8.1 [Source:ZFIN;Acc:ZDB-GENE-131127-4... [more]
back to top
BLAST of SMED30004730 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
B3SAF16.983e-840.74Predicted protein OS=Trichoplax adhaerens OX=10228... [more]
back to top
BLAST of SMED30004730 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30004730 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047424.12.366e-172100.00SMESG000047424.1[more]
SMESG000047424.12.305e-10498.68SMESG000047424.1[more]
SMESG000019784.11.003e-1436.89SMESG000019784.1[more]
SMESG000022965.11.469e-1242.00SMESG000022965.1[more]
SMESG000057093.11.198e-729.59SMESG000057093.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004730 ID=SMED30004730|Name=SMED30004730|organism=Schmidtea mediterranea sexual|type=transcript|length=1034bp
CCAATTTACCTTGTAAATTGGTATTAAATTTGAAAATCAAAATCCACAGA
TGTCAGACTTTGTGATCCATAAAATTTTGGGTTCCAACGAAATCGAAATT
ACTTTGTCTAAAGAAGAACGCGAAATATTGTTTAGCAAAGCTGAAAAATT
GAAGGAAAATGGTCTCACGGAAGACTCGGCATTTCAATGCTATAAGTGCT
TAAGAAATCTTACTTGCAATGAAGTTTTTCCAAGTCTTCCGAAGTGTTTG
AAATTACTGACCTTATACTTTTTCGAGAAGCAAAACTATATGATTGCGAT
TCAGCTTATCAACGCACTCAAATCTTATTACAAATCTGCACTTGATGAAG
CAGCATTAATTCACAGTAAAATAGTTGAATATTCTCAATTAAATGACGAA
TATGAAAATAAAAATATTAAGCTAAAAACTCCTGGCTCGTCTAAATCATT
TGCTGACCTTCAAAAAGAAAAGAATATAATTCAAAATGATCATAATGAAT
TAGATTTCGATTTTCGTTATTTAACATCTATTTTTTCCACTAAATTTGAA
GAAAACGAAGAAAGAGCGGAAATACTCAATCAAGCGGTCTCTGTTCAATC
CAGTCCAGATGTTTGCATGCTTATAAACAATTTAACTGCAATCGGAGACA
CTGAAAACTTATCAACAGTCGTTCAAGAGCAAAGCAGTTACTTAGACATC
ACTCGAGATTCTAACATGGAATCAAATGTAACAATCGAAAATCAGGGAGA
CAACCCAGTACATGAATCTAATTCAGAAGAAAATTCTACTCAATATATTT
TAGGAAGGTTAGTTTCGATTGCTGGCTACGTGTTTCTTATGGTAGTCGGA
ATCACAATTATTATATACTCCATGGAATTCTGCTTATTGTTTAATGAATC
TAATAAAAGTATTTCAAAACCACTCAATTCAAAAAGTCAAATTTCTTCCA
AATCAAAAATAATTAAATACATTCAAGAAAGAATTTTTCAGTTTCCGAAT
TATTTCTTTAAGAAATCAATGGAGTAAAACCAGT
back to top

protein sequence of SMED30004730-orf-1

>SMED30004730-orf-1 ID=SMED30004730-orf-1|Name=SMED30004730-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=326bp
MSDFVIHKILGSNEIEITLSKEEREILFSKAEKLKENGLTEDSAFQCYKC
LRNLTCNEVFPSLPKCLKLLTLYFFEKQNYMIAIQLINALKSYYKSALDE
AALIHSKIVEYSQLNDEYENKNIKLKTPGSSKSFADLQKEKNIIQNDHNE
LDFDFRYLTSIFSTKFEENEERAEILNQAVSVQSSPDVCMLINNLTAIGD
TENLSTVVQEQSSYLDITRDSNMESNVTIENQGDNPVHESNSEENSTQYI
LGRLVSIAGYVFLMVVGITIIIYSMEFCLLFNESNKSISKPLNSKSQISS
KSKIIKYIQERIFQFPNYFFKKSME*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 258..280