Glutathione S-transferase

Overview
NameGlutathione S-transferase
Smed IDSMED30004710
Length (bp)700
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glutathione S-transferase (SMED30004710) t-SNE clustered cells

Violin plots show distribution of expression levels for Glutathione S-transferase (SMED30004710) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30004710

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30004710SMESG000066038.1 SMESG000066037.1 Contig13104newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004710SMESG000066038.1 SMESG000066037.1 Contig13104uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0087005)

HSP 1 Score: 46.2098 bits (108), Expect = 6.846e-6
Identity = 43/174 (24.71%), Postives = 83/174 (47.70%), Query Frame = 3 
Query:  135 YEDIRLSDD-------NTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDK-----NKEM---YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            YED+R++ D           G+ P  +   +GK + +++++A+F AK     G   W+   I+  +  I+   RL  ++++ + E    +K     N E+   YL  +E+ + +N  G L    +T  D+YF  + +Y+ +      LE +P    V + + A  P +K ++EK
Sbjct:   75 YEDVRVTRDEWPALKPTMPMGQMP--VLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDF-RLKIAVVSYEPEDEIKEKKLVTLNAEVIPFYLEKLEQTVKDN-DGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEP-IKAWIEK 243
[Close]
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0100258)

HSP 1 Score: 46.2098 bits (108), Expect = 6.846e-6
Identity = 43/174 (24.71%), Postives = 83/174 (47.70%), Query Frame = 3 
Query:  135 YEDIRLSDD-------NTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDK-----NKEM---YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            YED+R++ D           G+ P  +   +GK + +++++A+F AK     G   W+   I+  +  I+   RL  ++++ + E    +K     N E+   YL  +E+ + +N  G L    +T  D+YF  + +Y+ +      LE +P    V + + A  P +K ++EK
Sbjct:   75 YEDVRVTRDEWPALKPTMPMGQMP--VLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDF-RLKIAVVSYEPEDEIKEKKLVTLNAEVIPFYLEKLEQTVKDN-DGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEP-IKAWIEK 243
[Close]
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0087006)

HSP 1 Score: 46.2098 bits (108), Expect = 6.846e-6
Identity = 43/174 (24.71%), Postives = 83/174 (47.70%), Query Frame = 3 
Query:  135 YEDIRLSDD-------NTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDK-----NKEM---YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            YED+R++ D           G+ P  +   +GK + +++++A+F AK     G   W+   I+  +  I+   RL  ++++ + E    +K     N E+   YL  +E+ + +N  G L    +T  D+YF  + +Y+ +      LE +P    V + + A  P +K ++EK
Sbjct:   75 YEDVRVTRDEWPALKPTMPMGQMP--VLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDF-RLKIAVVSYEPEDEIKEKKLVTLNAEVIPFYLEKLEQTVKDN-DGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEP-IKAWIEK 243
[Close]
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|O18598|GST1_BLAGE (Glutathione S-transferase OS=Blattella germanica OX=6973 PE=1 SV=3)

HSP 1 Score: 63.5438 bits (153), Expect = 5.229e-11
Identity = 43/173 (24.86%), Postives = 83/173 (47.98%), Query Frame = 3 
Query:  135 YEDIRLSDDNTHH-------GKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSI---LNRKCEISEWDKNKE----MYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            +ED R  + +  +       GK P  +   +GK   +++AI+++  K+    GKDDW+   I+  +  IS     +++     +   +  +WD  K+     Y +  ++++  NG G+L    +T  D YF+ +++YL        + N PN   + EK+L  LP +K ++ K
Sbjct:   30 FEDYRFQEGDWPNLKPSMPFGKTP--VLEIDGKQTHQSVAISRYLGKQFGLSGKDDWENLEIDMIVDTISDFRAAIANYHYDADENSKQKKWDPLKKETIPYYTKKFDEVVKANG-GYLAAGKLTWADFYFVAILDYLNHMAKEDLVANQPNLKALREKVLG-LPAIKAWVAK 198
[Close]
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P46428|GST_ANOGA (Glutathione S-transferase OS=Anopheles gambiae OX=7165 GN=GstS1 PE=2 SV=4)

HSP 1 Score: 53.1434 bits (126), Expect = 2.409e-7
Identity = 47/175 (26.86%), Postives = 85/175 (48.57%), Query Frame = 3 
Query:  135 YEDIRLSDDN------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEM----------YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            ++D+R++ +       T      P +E  +GK + +++A++++ A +    G DDW+   I+  +  ++   RL  +I+    E  +  K K+M          YL  +  +  EN +G LV    T  D+YF  +++YL + T +  LEN PN   V +K+L     VK Y+ K
Sbjct:   29 FDDVRITREEWPALKPTMPMGQMPVLE-VDGKKVHQSVAMSRYLANQVGLAGADDWENLMIDTVVDTVNDF-RLKIAIV--AYEPDDMVKEKKMVTLNNEVIPFYLTKLNVIAKEN-NGHLVLGKPTWADVYFAGILDYLNYLTKTNLLENFPNLQEVVQKVLDN-ENVKAYIAK 197
[Close]
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P46429|GST2_MANSE (Glutathione S-transferase 2 OS=Manduca sexta OX=7130 GN=GST2 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 2.790e-6
Identity = 50/184 (27.17%), Postives = 88/184 (47.83%), Query Frame = 3 
Query:  105 PITLIATG--------VVYEDIRLSDDNTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSI------LNRKCEISEWDKNKEMY-LRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTL-ENHPNFITVHEKILATLPQVKEYLE 608
            P  L+A G        V YE       NT  G+ P  +   +GK   ++LAI+++  ++    G D  + + I++ +  ++ I    +S+       N++ +  E  KNK  + L  + ++IT+N +GFL    +T  D  F+ M +YLK       L E +P F    E +L+  P++K YL+
Sbjct:   17 PTMLLAYGGQEFEDHRVEYEQWPEFKPNTPFGQMP--VLEIDGKKYAQSLAISRYLGRKYGLAGNDIEEDFEIDQIVDFVNDIRASAASVEYEQDAANKEVKHEENMKNKYPFQLNKLSEIITKN-NGFLALGRLTWADFVFVGMFDYLKKMLRMPDLEEQYPIFKKPIETVLSN-PKLKAYLD 196
[Close]
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P46437|GST_MUSDO (Glutathione S-transferase OS=Musca domestica OX=7370 PE=2 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 5.752e-6
Identity = 43/177 (24.29%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:  126 GVVYEDIRLSDD-------NTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDK-----NKEM---YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            G+ YED+R++ D           G+ P  +    GK + +++++A+F AK     G   W+   ++  +  I+   RL  ++++ + E    +K     N E+   YL  +E+ + +N  G L    +T  D+YF  +++Y+ +      LE +P    V + + A  P +K ++EK
Sbjct:   64 GIEYEDVRVTRDEWPALKPTMPMGQMP--VLEVNGKRVHQSISMARFLAKTVGLCGATPWEDLQVDIVVDTINDF-RLKIAVVSYEPEDEIKEKKLVTLNNEVIPFYLEKLEQTVKDN-DGHLALNKLTWADVYFAGILDYMNYMVKRDILEQYPALRGVVDSVNALEP-IKAWIEK 235
[Close]
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P26624|GST28_SCHJA (Glutathione S-transferase class-mu 28 kDa isozyme (Fragment) OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 8.916e-6
Identity = 46/196 (23.47%), Postives = 87/196 (44.39%), Query Frame = 3 
Query:   66 LYWNPKNPTHQLIPITLIATGVVYEDIRLSDDN------THHGKHPPYIE----NAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISE------WDKNKEMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYL 605
            +Y+N +    + I + L+A GV +ED R+   +      T  G   P ++      + K + ++LAIA+F A++ N MG  D + Y IEK I  +  +       L +  E  E       +    + L+ I + + E+     V   +TL D+  +  ++++           +P      + +LAT P++ +YL
Sbjct:    4 IYFNGRGRA-EPIRMILVAAGVEFEDERIEFQDWPKIKPTIPGGRLPIVKITDKRGDVKTMSESLAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESEYHKTLIKPPEEKEKISKEILNGKVPILLQAICETLKESTGNLTVGDKVTLADVVLIASIDHITDLDKEFLTGKYPEIHKHRKHLLATSPKLAKYL 198
[Close]
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A161MRG7 (Glutathione s-transferase-like protein OS=Triatoma infestans OX=30076 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 2.012e-13
Identity = 55/203 (27.09%), Postives = 96/203 (47.29%), Query Frame = 3 
Query:   45 RSVMSLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDN-------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEM-------YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            R++ + KL + P     + I   L      +EDIR   +N          GK P  +   +G  L ++ AI +++ K     GKD+W+   I+  +  I  + + +SS      E  +  K  E+       YL  +++L+  NG G+L    ++  DLYF+ + +YL         ENHPN  ++ + +LA LP++K ++EK
Sbjct:   25 RTMSTYKLTYFPVKALAEPIRFILSYMDKEFEDIRFEKENWPTIKPSMPFGKVP--VLEVDGIQLHQSTAITRYFGKEAGIAGKDNWEALQIDIAVDTIHDLRQAISSFWYETDENVKEKKRNEVLKDVVPFYLNKLDELVKTNG-GYLANSALSWGDLYFIAISDYLSHMAGIDIFENHPNLQSLKDTVLA-LPKIKAWIEK 223
[Close]
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A2A3E292 (Glutathione S-transferase OS=Apis cerana cerana OX=94128 GN=APICC_03799 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 6.143e-13
Identity = 53/196 (27.04%), Postives = 95/196 (48.47%), Query Frame = 3 
Query:   63 KLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDN-------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNK------EMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            KL + P     + I   L   G+ +ED R   D+       T  G+ P  + + +GK + +++AI+++ AK+    GKDDW+   I+  +  I  +   L++    + E  +  K K        YL  ++  +  NG G+ V   ++  DL F+ +++YL F   S  +E + N   + EK+L  LP +K +L+K
Sbjct:    5 KLTYFPVKGLGEPIRFLLSYAGISFEDERFDRDDWPKIKPTTPFGQVP--VLDVDGKKIAQSVAISRYLAKKSGLAGKDDWEALEIDSIVDTIHDVRARLAAFHYEENEEIKAAKRKIADEVVPYYLERLDAQVKNNG-GYFVGGALSWADLSFVALLDYLNFMNGSDLIEKYDNLKQLKEKVL-NLPAIKSWLDK 196
[Close]
BLAST of Glutathione S-transferase vs. TrEMBL
Match: V9IJF3 (Glutathione S-transferase OS=Apis cerana OX=7461 GN=ACCB11368 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 6.143e-13
Identity = 53/196 (27.04%), Postives = 95/196 (48.47%), Query Frame = 3 
Query:   63 KLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDN-------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNK------EMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            KL + P     + I   L   G+ +ED R   D+       T  G+ P  + + +GK + +++AI+++ AK+    GKDDW+   I+  +  I  +   L++    + E  +  K K        YL  ++  +  NG G+ V   ++  DL F+ +++YL F   S  +E + N   + EK+L  LP +K +L+K
Sbjct:    5 KLTYFPVKGLGEPIRFLLSYAGISFEDERFDRDDWPKIKPTTPFGQVP--VLDVDGKKIAQSVAISRYLAKKSGLAGKDDWEALEIDSIVDTIHDVRARLAAFHYEENEEIKAAKRKIADEVVPYYLERLDAQVKNNG-GYFVGGALSWADLSFVALLDYLNFMNGSDLIEKYDNLKQLKEKVL-NLPAIKSWLDK 196
[Close]
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A0M3JVD3 (Glutathione S-transferase class-mu 28 kDa isozyme (inferred by orthology to a S. mansoni protein) OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS11649 PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 9.397e-13
Identity = 50/188 (26.60%), Postives = 91/188 (48.40%), Query Frame = 3 
Query:   96 QLIPITLIATGVVYEDIRLSDDN-------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNK----------EMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLE 608
            ++I +      V YED+R++D +          G  P   E + G++L ++ AIA + AK+    G+DDWD   I++   L       ++  L    E +E +K K          E +   I+K++ +NG GF V K +++ DL  L M+     + F  T  N+P  I   +++++  P +K+++E
Sbjct:   16 EVIRLLFAQANVDYEDVRITDSDWPALKPKVPFGHVPVLEEESTGRMLGESTAIALYLAKKFGLNGQDDWDAAKIQE---LFGATTDFVNHALPFYKETNEAEKKKLMVAFEKDHLEPFFTQIDKILQQNGTGFFVGKQLSVADLNMLCMIGLFG-SLFPTTANNYPQLIAFKDRMMSQ-PNIKKWIE 198
[Close]
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A2S2R8M3 (Glutathione S-transferase OS=Sipha flava OX=143950 GN=GST1_2 PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.101e-12
Identity = 48/173 (27.75%), Postives = 88/173 (50.87%), Query Frame = 3 
Query:  135 YEDIRLSDDN-------THHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEM-------YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            +ED R   +           GK P  +   +GKVL+++ AI ++ +K+    G DDW+   I+  +  +  + + ++S      E S+  K + +       Y+   EK++ EN +G+ V   +T  DL+F+ +++YL F      LE  PN   + +K+LA +PQ+K ++EK
Sbjct:   29 FEDFRFDKEQWPSIKPTMPFGKVP--VVEIDGKVLNQSTAICRYLSKKAGLAGSDDWESMLIDIAVDNVHDLRQAIASYKYDPLEESKKTKYEPLIKETIPFYMDKFEKIVGEN-NGYFVNGKLTWADLFFVAILDYLNFMAEIDLLEGRPNMQALKKKVLA-VPQIKTWVEK 197
[Close]
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO45159 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTW9])

HSP 1 Score: 46.9802 bits (110), Expect = 1.280e-6
Identity = 53/205 (25.85%), Postives = 85/205 (41.46%), Query Frame = 3 
Query:   57 SLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGK---HPPYIENAEGKV----------LDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGIS-----LISKIVRLLSSILNRKCEISEWDKNKEM--YLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEK 611
            S KLY+       +   +   A G+ +ED R++       K   H P+     G+V          L ++LAI  F A+       +  D+   +  +      L  KI         +K E+ E  +N  +  ++  + K++ ENG GFLVT  +T  D+      N L     S  L+  P     H++I A+ P +K YLEK
Sbjct:    3 SYKLYYFNARGRAEPARLVFAAAGIEFEDNRMAMGEWPKVKKELHAPF-----GQVPLLVIDDKIKLAQSLAIMTFIAREGGLAPSNSLDIAKCDMIVGAEEDFLTKKIQCHFEKDEAKKKELDEAIQNTHLPAFMGALTKILEENGTGFLVTDKMTYADIIVFNACNNLD----SGALDKFPKLKQHHDRI-ASNPGIKAYLEK 197
[Close]
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000066038.1 (SMESG000066038.1)

HSP 1 Score: 385.956 bits (990), Expect = 2.290e-138
Identity = 187/187 (100.00%), Postives = 187/187 (100.00%), Query Frame = 3 
Query:   54 MSLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEKP 614
            MSLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEKP
Sbjct:    1 MSLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEKP 187
[Close]
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000032177.1 (SMESG000032177.1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.276e-15
Identity = 41/177 (23.16%), Postives = 88/177 (49.72%), Query Frame = 3 
Query:   96 QLIPITLIATGVVYEDIRLSDDNTHHGKHP------PYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNK-EMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYL 605
            +++ +   A  + +ED R +++     K        P +EN +G++L +  AI +FY+  +  +G  DW+ Y IEK I    +   +L+ ++ +   + +  ++     L+ + K+   N +GFLV K  +L D+Y LV++++ +     +          V +K+L T P++  ++
Sbjct:   17 EVLRLLFKAANIKFEDKRYTEEEWETAKSAFKIKELPLVENYDGRLLTEPSAICRFYSLNRGLLGSSDWEYYMIEKCIEHADESCHILNDVVFKNITLDDHHEHALSSSLKTLNKVAEVNKNGFLVGKTASLADIYLLVLLDFAEMRAAKEHFTRFKKLHEVKKKLLNTFPEINTWI 193
[Close]
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000003087.1 (SMESG000003087.1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.140e-14
Identity = 52/183 (28.42%), Postives = 92/183 (50.27%), Query Frame = 3 
Query:   96 QLIPITLIATGVVYEDIRLSDD-------NTHHGKHPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSILNRKCEISEWDKNKEMY------LRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEYL 605
            +LI + L A+ + +ED+R++ D        T  GK P  +   EGK L +++AIA+  A++   MG+DD + Y +E+ I  +  ++  L  I     E  E  + + +       L+ +E  I E   GF   + ITL +L+FLV+ +YL      +  ++ P    + E+IL     V+E++
Sbjct:   16 ELIRLVLKASKIEFEDVRITKDKWPEVKPTTPTGKLP--VVEYEGKQLTQSMAIARVVARKHGFMGEDDKEYYLVERAIGQMVDVLEGLYKIYFAPEEKKEELRAEYVATSGRDNLKALEGFIKET--GFFAGEKITLAELFFLVVSDYL--VKLPQLYDDFPKLKELRERILKANTDVEEWV 192
[Close]
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000016846.1 (SMESG000016846.1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.640e-11
Identity = 44/191 (23.04%), Postives = 96/191 (50.26%), Query Frame = 3 
Query:   69 YWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGK------HPPYIENAEGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGI----SLISKIVRLLSSILNRKCEISEWDKNK---EMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNYLKFTTFSKTLENHPNFITVHEKILATLPQVKEY 602
            Y+N K    +LI + L+A+ V +ED R+  ++    K        P ++   G +L+++LAIA+++AK+ +  G  + + Y  ++ +     L +KIV +       K ++   + N+    ++L  +    TE+   F +    +  DL  + +M++ + + +    +N+P  +  ++++L   P+VKEY
Sbjct:    9 YFNAKAKA-ELIRLILVASNVDFEDKRIEFEDWPQLKPSIPSGQLPVVQLTCGTILNESLAIARYFAKKYHLAGSGEIEEYKADRAVCTLDDLFTKIVDIFHEKDVEKSKLLRTELNETHLAIFLNRVSGYFTEHDGDFFLGSKPSYADLQLVNIMDHFEESQY----QNYPKIVKCYQQVLEHFPKVKEY 194
The following BLAST results are available for this feature:
BLAST of Glutathione S-transferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
GstS16.846e-624.71gene:FBgn0010226 transcript:FBtr0087005[more]
GstS16.846e-624.71gene:FBgn0010226 transcript:FBtr0100258[more]
GstS16.846e-624.71gene:FBgn0010226 transcript:FBtr0087006[more]
back to top
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O18598|GST1_BLAGE5.229e-1124.86Glutathione S-transferase OS=Blattella germanica O... [more]
sp|P46428|GST_ANOGA2.409e-726.86Glutathione S-transferase OS=Anopheles gambiae OX=... [more]
sp|P46429|GST2_MANSE2.790e-627.17Glutathione S-transferase 2 OS=Manduca sexta OX=71... [more]
sp|P46437|GST_MUSDO5.752e-624.29Glutathione S-transferase OS=Musca domestica OX=73... [more]
sp|P26624|GST28_SCHJA8.916e-623.47Glutathione S-transferase class-mu 28 kDa isozyme ... [more]
back to top
BLAST of Glutathione S-transferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A161MRG72.012e-1327.09Glutathione s-transferase-like protein OS=Triatoma... [more]
A0A2A3E2926.143e-1327.04Glutathione S-transferase OS=Apis cerana cerana OX... [more]
V9IJF36.143e-1327.04Glutathione S-transferase OS=Apis cerana OX=7461 G... [more]
A0A0M3JVD39.397e-1326.60Glutathione S-transferase class-mu 28 kDa isozyme ... [more]
A0A2S2R8M31.101e-1227.75Glutathione S-transferase OS=Sipha flava OX=143950... [more]
back to top
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO451591.280e-625.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Glutathione S-transferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Glutathione S-transferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000066038.12.290e-138100.00SMESG000066038.1[more]
SMESG000032177.13.276e-1523.16SMESG000032177.1[more]
SMESG000003087.13.140e-1428.42SMESG000003087.1[more]
SMESG000016846.13.640e-1123.04SMESG000016846.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004710 ID=SMED30004710|Name=Glutathione S-transferase|organism=Schmidtea mediterranea sexual|type=transcript|length=700bp
ATTTGGAATAAAATCTATCCGAAATGAAAATTTGATTAAAAATTCGAAGT
GTAATGTCTTTGAAACTGTATTGGAACCCAAAGAATCCTACTCATCAACT
GATTCCCATTACTCTAATTGCAACTGGTGTTGTGTATGAAGATATTAGAT
TATCCGATGACAATACTCATCATGGAAAGCACCCTCCGTACATTGAAAAT
GCAGAAGGGAAAGTCTTGGATAAAACGCTAGCGATAGCCAAATTTTATGC
AAAACGAAAGAATTGCATGGGGAAAGATGATTGGGATCTTTATTGCATTG
AAAAAGGGATAAGTTTGATTAGCAAAATCGTAAGGCTTCTCAGTAGCATA
TTAAACAGAAAATGCGAAATAAGTGAATGGGATAAAAACAAAGAAATGTA
TTTGCGGCATATTGAAAAGTTGATTACCGAAAATGGTCATGGGTTTCTTG
TCACAAAAGGAATAACGCTAACAGATTTATACTTTTTGGTCATGATGAAT
TATTTGAAATTCACCACATTTTCTAAGACTTTGGAAAATCATCCAAATTT
CATTACGGTCCATGAAAAAATACTAGCAACTCTGCCCCAAGTTAAGGAAT
ATTTGGAAAAGCCTTAAAATTTTTCTTTGCCATTAATTTTCTAAATAAAT
AACAACTTTTCATTCTTTTGTTAATAAATTATACATTCGTTTAAAAAAAA
back to top

protein sequence of SMED30004710-orf-1

>SMED30004710-orf-1 ID=SMED30004710-orf-1|Name=SMED30004710-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=188bp
MSLKLYWNPKNPTHQLIPITLIATGVVYEDIRLSDDNTHHGKHPPYIENA
EGKVLDKTLAIAKFYAKRKNCMGKDDWDLYCIEKGISLISKIVRLLSSIL
NRKCEISEWDKNKEMYLRHIEKLITENGHGFLVTKGITLTDLYFLVMMNY
LKFTTFSKTLENHPNFITVHEKILATLPQVKEYLEKP*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR036282Glutathione-S-Trfase_C_sf
IPR010987Glutathione-S-Trfase_C-like
IPR012336Thioredoxin-like_fold
IPR004046GST_C
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1050.10coord: 70..176
e-value: 1.6E-21
score: 79.2
NoneNo IPR availablePANTHERPTHR11571:SF206HEMATOPOIETIC PROSTAGLANDIN D SYNTHASEcoord: 15..186
NoneNo IPR availablePANTHERPTHR11571GLUTATHIONE S-TRANSFERASEcoord: 15..186
IPR004046Glutathione S-transferase, C-terminalPFAMPF14497GST_C_3coord: 111..186
e-value: 1.9E-11
score: 44.0
IPR036249Thioredoxin-like superfamilyGENE3DG3DSA:3.40.30.10coord: 16..186
e-value: 1.6E-21
score: 79.2
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILYSSF47616GST C-terminal domain-likecoord: 88..186