SMED30004699

Overview
NameSMED30004699
Smed IDSMED30004699
Length (bp)3046
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004699

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30004699SMESG000019255.1 Contig27491newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004699SMESG000019255.1 Contig27491uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
testis primordiumSMED30004699SMESG000019255.1 PL030002A10A02_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30004699 vs. Ensembl Human
Match: SETMAR (SET domain and mariner transposase fusion gene [Source:HGNC Symbol;Acc:HGNC:10762])

HSP 1 Score: 52.373 bits (124), Expect = 7.932e-15
Identity = 36/99 (36.36%), Postives = 53/99 (53.54%), Query Frame = -3 
Query:  585 STYKILLKTPMKIM----SVYKVHLEHFMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQET 869
            S  ++ L+T MK+M     +  + L  F   RK  A   + N+   FG G  N RT Q  F+K    D SLE++ + GRPS++DN+ L  I+E +P  T
Sbjct:  333 SCKRLTLET-MKMMLDKKQIRAIFLFEFKMGRK--AAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTT 428

HSP 2 Score: 49.6766 bits (117), Expect = 7.932e-15
Identity = 24/77 (31.17%), Postives = 46/77 (59.74%), Query Frame = -2 
Query:  274 WLKNNLSD-----RFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPGTSLATLEK*DIHQQKVLLKV 489
            W+ + L++     RF + SS ++RN+ EPFLD+++T DEK+ LY+N ++   +     +     K  +H +KV++ +
Sbjct:  459 WVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTI 535
[Close]
BLAST of SMED30004699 vs. Ensembl Human
Match: SETMAR (SET domain and mariner transposase fusion gene [Source:HGNC Symbol;Acc:HGNC:10762])

HSP 1 Score: 52.373 bits (124), Expect = 8.109e-15
Identity = 36/99 (36.36%), Postives = 53/99 (53.54%), Query Frame = -3 
Query:  585 STYKILLKTPMKIM----SVYKVHLEHFMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQET 869
            S  ++ L+T MK+M     +  + L  F   RK  A   + N+   FG G  N RT Q  F+K    D SLE++ + GRPS++DN+ L  I+E +P  T
Sbjct:  194 SCKRLTLET-MKMMLDKKQIRAIFLFEFKMGRK--AAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTT 289

HSP 2 Score: 49.2914 bits (116), Expect = 8.109e-15
Identity = 24/78 (30.77%), Postives = 46/78 (58.97%), Query Frame = -2 
Query:  271 WLKNNLSD-----RFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPGTSLATLEK*DIHQQKVLLKVL 489
            W+ + L++     RF + SS ++RN+ EPFLD+++T DEK+ LY+N ++   +     +     K  +H +KV++ + 
Sbjct:  320 WVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTIW 397
[Close]
BLAST of SMED30004699 vs. Ensembl Human
Match: SETMAR (SET domain and mariner transposase fusion gene [Source:HGNC Symbol;Acc:HGNC:10762])

HSP 1 Score: 52.373 bits (124), Expect = 8.637e-7
Identity = 36/99 (36.36%), Postives = 53/99 (53.54%), Query Frame = -3 
Query:  585 STYKILLKTPMKIM----SVYKVHLEHFMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQET 869
            S  ++ L+T MK+M     +  + L  F   RK  A   + N+   FG G  N RT Q  F+K    D SLE++ + GRPS++DN+ L  I+E +P  T
Sbjct:   97 SCKRLTLET-MKMMLDKKQIRAIFLFEFKMGRK--AAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTT 192
[Close]
BLAST of SMED30004699 vs. UniProt/SwissProt
Match: sp|Q53H47|SETMR_HUMAN (Histone-lysine N-methyltransferase SETMAR OS=Homo sapiens OX=9606 GN=SETMAR PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 3.507e-14
Identity = 36/99 (36.36%), Postives = 53/99 (53.54%), Query Frame = -3 
Query:  585 STYKILLKTPMKIM----SVYKVHLEHFMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQET 869
            S  ++ L+T MK+M     +  + L  F   RK  A   + N+   FG G  N RT Q  F+K    D SLE++ + GRPS++DN+ L  I+E +P  T
Sbjct:  333 SCKRLTLET-MKMMLDKKQIRAIFLFEFKMGRK--AAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTT 428

HSP 2 Score: 49.6766 bits (117), Expect = 3.507e-14
Identity = 24/77 (31.17%), Postives = 46/77 (59.74%), Query Frame = -2 
Query:  274 WLKNNLSD-----RFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPGTSLATLEK*DIHQQKVLLKV 489
            W+ + L++     RF + SS ++RN+ EPFLD+++T DEK+ LY+N ++   +     +     K  +H +KV++ +
Sbjct:  459 WVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTI 535
[Close]
BLAST of SMED30004699 vs. TrEMBL
Match: A0A2P6JUP7 (Histone-lysine N-methyltransferase SETMAR OS=Nephila clavipes OX=2585209 GN=NCL1_63062 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.269e-24
Identity = 51/82 (62.20%), Postives = 64/82 (78.05%), Query Frame = -2 
Query:  232 NLSDRFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPGTSLATLEK*DIHQQKVLLKVLRFYETRKNYLSRQ 477
            NLSDR RICSS+L+R NMEPFLDKLITGD+K+ LYENIK++K YCKPGTS AT+ K  IH QKV L +   ++ ++  +S Q
Sbjct:   49 NLSDRVRICSSHLIRLNMEPFLDKLITGDKKWILYENIKRKKSYCKPGTSSATIRKPSIHHQKVQLCLWWEHKRKRATISVQ 130

HSP 2 Score: 39.6614 bits (91), Expect = 1.269e-24
Identity = 15/30 (50.00%), Postives = 24/30 (80.00%), Query Frame = -1 
Query:  479 ISLTVTGDYIKALMFVLKKAVWIPYDLAKK 568
            I  T  GD+IK+L FV+K++VW+P++L +K
Sbjct:   19 IHHTTVGDHIKSLAFVVKRSVWVPHELTEK 48
[Close]
BLAST of SMED30004699 vs. TrEMBL
Match: A0A087SYD3 (Mariner Mos1 transposase (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_01310 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.612e-20
Identity = 42/66 (63.64%), Postives = 55/66 (83.33%), Query Frame = -2 
Query:  280 NLSDRFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPGTSLATLEK*DIHQQKVLL 477
            NLSDR R+CSS+L+R+N+E FL++L+TGDEK+ LYENIK +K YCKPG S AT+ K  IH++KVL 
Sbjct:   49 NLSDRVRMCSSHLIRHNVESFLNRLVTGDEKWILYENIKSKKSYCKPGISSATVPKPSIHERKVLF 114

HSP 2 Score: 35.8094 bits (81), Expect = 1.612e-20
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = -1 
Query:  479 ISLTVTGDYIKALMFVLKKAVWIPYDLAKK 568
            I  T  GD+IK+L FVLK++V +P++L +K
Sbjct:   19 IHHTTVGDHIKSLGFVLKQSVCVPHELTEK 48

HSP 3 Score: 22.3274 bits (46), Expect = 1.612e-20
Identity = 16/59 (27.12%), Postives = 24/59 (40.68%), Query Frame = -3 
Query:  156 FYDFMKQGKTI----YLGNIVIL---------------GLVFSHDTYFAFVSLVLKHSL 275
            +Y+ +KQGKTI    Y   +  L               G+VF HD      ++V +  L
Sbjct:  125 YYELLKQGKTINVDLYCNQLDKLNAAIKEKRPALASRKGIVFHHDNARPPTAMVTQQKL 183
[Close]
BLAST of SMED30004699 vs. TrEMBL
Match: A0A1I7X3J6 (HTH_48 domain-containing protein OS=Heterorhabditis bacteriophora OX=37862 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.531e-20
Identity = 28/48 (58.33%), Postives = 38/48 (79.17%), Query Frame = -2 
Query:  334 NLSDRFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPG 477
            NL DR  IC+S L RN +EPFL+++ITGDEK+  YE+I ++K YC+PG
Sbjct:  111 NLDDRVVICTSLLARNKIEPFLNRMITGDEKWITYESIVRQKAYCEPG 158

HSP 2 Score: 62.3882 bits (150), Expect = 8.531e-20
Identity = 32/82 (39.02%), Postives = 48/82 (58.54%), Query Frame = -3 
Query:  594 MSVYKVHLEH---FMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNP 830
            M+  KVHL H   +   + +NA  A  NL  +FG G V+ RTC+R F +  + D  L +  + GRPS ID++V+  +LE +P
Sbjct:    1 MATAKVHLRHCILYEFQQGRNATEACRNLLKVFGEGSVSDRTCRRWFERFGTGDFDLYDKLRSGRPSLIDDDVVKTMLEQDP 82
[Close]
BLAST of SMED30004699 vs. TrEMBL
Match: A0A088APF9 (HTH_48 domain-containing protein OS=Apis mellifera OX=7460 PE=4 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.402e-19
Identity = 28/50 (56.00%), Postives = 38/50 (76.00%), Query Frame = -2 
Query:  334 KNNLSDRFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPG 483
            + NL DR  IC+S L RN +EPFL+++ITGDEK+  Y NI K++ YC+PG
Sbjct:  122 QKNLDDRVVICTSLLARNKIEPFLNRMITGDEKWITYNNIVKKRAYCEPG 171

HSP 2 Score: 57.3806 bits (137), Expect = 1.402e-19
Identity = 34/95 (35.79%), Postives = 53/95 (55.79%), Query Frame = -3 
Query:  555 MSVYKVHLEH---FMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQETLVQYQNFSNS 830
            M+  K HL H   +   + +NA  A  NL  +FG G+V+  TC+R + K  + D +L  D + GRPS ID++V+  +LE +P  T+ +     NS
Sbjct:    1 MATDKNHLRHCILYEFQQGRNATEACRNLLKVFGEGIVSDMTCRRWYEKFETSDFNL-SDKRSGRPSLIDDDVVKAMLEQDPFLTISEIAQRLNS 94

HSP 3 Score: 23.483 bits (49), Expect = 1.402e-19
Identity = 8/24 (33.33%), Postives = 17/24 (70.83%), Query Frame = -1 
Query:  479 GDYIKALMFVLKKAVWIPYDLAKK 550
             D+I+ +  V K + W+P++L++K
Sbjct:  100 SDHIRKIGLVWKYSRWVPHELSQK 123
[Close]
BLAST of SMED30004699 vs. TrEMBL
Match: A0A088A6P4 (HTH_48 domain-containing protein OS=Apis mellifera OX=7460 PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 2.188e-19
Identity = 28/50 (56.00%), Postives = 38/50 (76.00%), Query Frame = -2 
Query:  334 KNNLSDRFRICSSYLVRNNMEPFLDKLITGDEKYNLYENIKKRKFYCKPG 483
            + NL DR  IC+S L RN +EPFL+++ITGDEK+  Y NI K++ YC+PG
Sbjct:  122 QKNLDDRVVICTSLLARNKIEPFLNRMITGDEKWITYNNIVKKRAYCEPG 171

HSP 2 Score: 57.7658 bits (138), Expect = 2.188e-19
Identity = 34/95 (35.79%), Postives = 53/95 (55.79%), Query Frame = -3 
Query:  555 MSVYKVHLEH---FMRSRKKNALIASENLCAIFGRGVVNVRTCQR*FRKLRSEDLSLEEDTKPGRPSKIDNEVLWFILEHNPQETLVQYQNFSNS 830
            M+  K HL H   +   + +NA  A  NL  +FG G+V+  TC+R + K  + D +L  D + GRPS ID++V+  +LE +P  T+ +     NS
Sbjct:    1 MATDKNHLRHCILYEFQQGRNATEACRNLLKVFGEGIVSDMTCRRWYEKFETSDFNL-SDKRSGRPSLIDDDVVKAMLEQDPFLTISEIAQRLNS 94

HSP 3 Score: 23.483 bits (49), Expect = 2.188e-19
Identity = 8/24 (33.33%), Postives = 17/24 (70.83%), Query Frame = -1 
Query:  479 GDYIKALMFVLKKAVWIPYDLAKK 550
             D+I+ +  V K + W+P++L++K
Sbjct:  100 SDHIRKIGLVWKYSRWVPHELSQK 123
[Close]
BLAST of SMED30004699 vs. Planmine SMEST
Match: SMESG000019255.1 (SMESG000019255.1)

HSP 1 Score: 1166.37 bits (3016), Expect = 0.000e+0
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 3 
Query: 1209 MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQYIVVTLYDIFQKYRMALRFDEEKATRFESSSDYLKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIEAQMRANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRALYGNDLQRNRKNVEINDKGLDSQRPYYGSPNDVDPNQGNQQRKNCKTPQNSGKSDVSNGGRQYRKNLNVNPNDAGPYNYQGNVKMNNPINPQDSINSEINDTSRQYQRTYKDAQDDVDPYNSQGNRKMKSSVNPQNSTISDLNGSRQKRIQDSVLDDIVRRIENRQAYGKEITGIPFNRRAKSNSEAVGLPNQRDSQSTGLPPQRRARSSNEATGLPDERDFQYNPESRQKPVKTGCGGSGACLKQNNXXXXXXXXXXXXXXXXXXXXAGRVHQHGHKHNNQGKSSSVRGDIXXXXXXXXXXXXVEIDSHTKYLHSASDRNDQVATDNIKLDSLSTSSLKPEELNRRSPAHY 3005
            MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQYIVVTLYDIFQKYRMALRFDEEKATRFESSSDYLKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIEAQMRANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRALYGNDLQRNRKNVEINDKGLDSQRPYYGSPNDVDPNQGNQQRKNCKTPQNSGKSDVSNGGRQYRKNLNVNPNDAGPYNYQGNVKMNNPINPQDSINSEINDTSRQYQRTYKDAQDDVDPYNSQGNRKMKSSVNPQNSTISDLNGSRQKRIQDSVLDDIVRRIENRQAYGKEITGIPFNRRAKSNSEAVGLPNQRDSQSTGLPPQRRARSSNEATGLPDERDFQYNPESRQKPVKTGCGGSGACLKQNNSSRDSSKRGSSVKTSCTSSSAGRVHQHGHKHNNQGKSSSVRGDISKNQNSSKSDKKVEIDSHTKYLHSASDRNDQVATDNIKLDSLSTSSLKPEELNRRSPAHY
Sbjct:    1 MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQYIVVTLYDIFQKYRMALRFDEEKATRFESSSDYLKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIEAQMRANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRALYGNDLQRNRKNVEINDKGLDSQRPYYGSPNDVDPNQGNQQRKNCKTPQNSGKSDVSNGGRQYRKNLNVNPNDAGPYNYQGNVKMNNPINPQDSINSEINDTSRQYQRTYKDAQDDVDPYNSQGNRKMKSSVNPQNSTISDLNGSRQKRIQDSVLDDIVRRIENRQAYGKEITGIPFNRRAKSNSEAVGLPNQRDSQSTGLPPQRRARSSNEATGLPDERDFQYNPESRQKPVKTGCGGSGACLKQNNSSRDSSKRGSSVKTSCTSSSAGRVHQHGHKHNNQGKSSSVRGDISKNQNSSKSDKKVEIDSHTKYLHSASDRNDQVATDNIKLDSLSTSSLKPEELNRRSPAHY 599
[Close]
BLAST of SMED30004699 vs. Planmine SMEST
Match: SMESG000019255.1 (SMESG000019255.1)

HSP 1 Score: 312.383 bits (799), Expect = 7.329e-97
Identity = 148/251 (58.96%), Postives = 188/251 (74.90%), Query Frame = 3 
Query: 1209 MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQYIVVTLYDIFQKYRMALRFDEEKATRFESSSDY---LKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIEAQMRANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRAL 1952
            MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQL G+ +K  G ALP  DP R N++  G+AEKLIGQMENE++   N L+LK IQE RLYHQ  ++ +   F +    L+ +E++A+    +S+    L+N R++++  H  LH +VI ++E LN++K  LPA+IE Q+  N Y +NYLEY+LE+FR NK+I+  I PL S  +E+   VPDYR ++
Sbjct:    1 MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNVDTLPDKLRISWFQLIGKSIKTAGQALPEKDPIRSNVITTGRAEKLIGQMENEFAAFCNALLLKPIQEARLYHQESIIGINKEFIQIEKKLKIEEQEASLGNLTSERIQGLENLRNDYQGAHYALHSQVIRIYEVLNQIKLRLPAVIEYQLDMNQYVSNYLEYVLESFRGNKEIKREIKPLKSYHAEIQSKVPDYRESI 251
[Close]
BLAST of SMED30004699 vs. Planmine SMEST
Match: SMESG000016678.1 (SMESG000016678.1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.223e-17
Identity = 75/246 (30.49%), Postives = 117/246 (47.56%), Query Frame = 3 
Query: 1287 EEHLETMKFITQTGKVLEKTVYQ--NFNVDT---LPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQ---YI----VVTLYDIFQK--YRMALRFDEEKATRFESSSDYLKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIE-AQMRANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRALYGNDLQRNR 1979
            ++H   +  + + G++LE  +Y   + N  T   + +K RIS FQ+ GQC+K     LP  DP+RE +   GK EKLIGQ ENE    +N LIL  IQ +R   +   Y+    +  L+D   +   R +   D E   R E     +K    E  N+  +L + V              P IIE A+   N   N  LEY++  F+DN+ +++ +    ++  E T  V D+R  +Y N   +N+
Sbjct:   22 QQHQIGLFHLNKIGRLLESIIYHSGDGNATTFINITNKTRISNFQILGQCMKKISSTLPKGDPFREVLNLCGKIEKLIGQFENELIHTLNYLILAPIQRIRCNQREKIYVYNLELTHLWDHIHRDEIRDSSEEDPELKLRAEFLQGEIKRALKESNNMMIELRNNV-------------YPFIIEHAKCSENIIKN--LEYLIMTFKDNRSMKHIVDISDTISVEST--VEDFRERIYKNMTDQNK 250
[Close]
BLAST of SMED30004699 vs. Planmine SMEST
Match: SMESG000016679.1 (SMESG000016679.1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.062e-15
Identity = 47/131 (35.88%), Postives = 75/131 (57.25%), Query Frame = 3 
Query: 1209 MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQNFNV----DTLPDKL-RISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIGQMENEYSTIINCLILKNIQELRLYHQ 1586
            MGN+E IY  KC V  P+    C + + H   M FI   G+VLEK +Y + NV    + + D+  R S +Q++G CLK  GL +     Y+  ++  G+ EK++GQ+EN     +N L L  IQ++R++++
Sbjct:    1 MGNSE-IYYGKCEVAEPNIYSLCEMLKMHKFKMHFIEYLGEVLEKLIYTSPNVINGINIIDDESGRTSLYQIFGDCLKKAGLMMEDDYSYKSILILVGRTEKMMGQIENNCLYHLNSLFLVPIQKIRMFNR 130
The following BLAST results are available for this feature:
BLAST of SMED30004699 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SETMAR7.932e-1536.36SET domain and mariner transposase fusion gene [So... [more]
SETMAR8.109e-1536.36SET domain and mariner transposase fusion gene [So... [more]
SETMAR8.637e-736.36SET domain and mariner transposase fusion gene [So... [more]
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BLAST of SMED30004699 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q53H47|SETMR_HUMAN3.507e-1436.36Histone-lysine N-methyltransferase SETMAR OS=Homo ... [more]
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BLAST of SMED30004699 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2P6JUP71.269e-2462.20Histone-lysine N-methyltransferase SETMAR OS=Nephi... [more]
A0A087SYD31.612e-2063.64Mariner Mos1 transposase (Fragment) OS=Stegodyphus... [more]
A0A1I7X3J68.531e-2058.33HTH_48 domain-containing protein OS=Heterorhabditi... [more]
A0A088APF91.402e-1956.00HTH_48 domain-containing protein OS=Apis mellifera... [more]
A0A088A6P42.188e-1956.00HTH_48 domain-containing protein OS=Apis mellifera... [more]
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BLAST of SMED30004699 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004699 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000019255.10.000e+0100.00SMESG000019255.1[more]
SMESG000019255.17.329e-9758.96SMESG000019255.1[more]
SMESG000016678.12.223e-1730.49SMESG000016678.1[more]
SMESG000016679.11.062e-1535.88SMESG000016679.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004699 ID=SMED30004699|Name=SMED30004699|organism=Schmidtea mediterranea sexual|type=transcript|length=3046bp
AAAATACCACAAATTTTGATTTTGTTTATTATTGTTTTATTGAGTAAAAA
TAAATTTAATTGAAGATCATTCATTAAATTTTTTTAATTTAATTTTTGTA
CATGTATATTATGTTTTTCCTTTTTTTCTTGGAATCCAATAATTAAGTAG
AGCTACAAACTATGCTTTAAAACCAAACTAACAAACGCAAAGTATGTGTC
ATGACTGAAAACTAGGCCCAGAATTACAATGTTGCCTAGATAGATAGTTT
TTCCTTGTTTCATAAAATCGTAAAACTTTTAGCAAAACTTTCTGCTGATG
AATATCTTACTTTTCAAGAGTTGCTAGTGATGTTCCAGGTTTGCAGTAAA
ATTTTCTCTTCTTTATGTTCTCGTAAAGATTATACTTTTCATCTCCAGTG
ATCAGTTTATCCAGAAACGGCTCCATATTATTTCTAACTAGATATGATGA
ACATATTCTAAAACGGTCAGACAAGTTATTTTTTAGCCAAATCATATGGG
ATCCAAACAGCTTTCTTTAAGACAAACATAAGTGCTTTAATGTAATCTCC
AGTAACTGTTAGAGAAATTCTGATATTGCACCAATGTTTCTTGTGGATTA
TGTTCCAATATAAACCACAAAACTTCATTATCGATCTTGGATGGTCGTCC
AGGTTTAGTATCCTCTTCTAGGCTCAGATCTTCGGAACGTAATTTTCTAA
ATCATCTTTGACAAGTACGAACATTTACAACACCTCTTCCAAATATTGCA
CAAAGGTTTTCACTTGCAATCAACGCGTTTTTTTTTCTTGATCTCATAAA
ATGTTCCAAATGCACTTTATACACTGACATTATTTTCATTGGCGTTTTTA
ACAGAATCTTGTAAGTTGAAGCGTTGTTGAGCAAAATATTGTTGTGGGTT
TTGAAAAAGTGATAGTTAGTTGGCAAGATATGATGTGATATTATTTCTGA
TGTATCTTGTTGATTTATCGAGAGCATTTTTCTGCAGTACCGGTTTCGCC
TAGATTTAGGAGATCATAGTGAATCAGCCCAGCCGTACAACACCAAAACT
TTATCATTACCTTTGGGCTCTGTGAAAAAACAGTCTTTATTTGAAAAAAA
GATTTATAGAACTTCAATAAAAAATCAAATGACCATTATTTTAGTAACAA
TCTAATAAATATAAAATATAAAATCAATTATAAATAATATATTGTCGATC
AAATAATTATGGGAAATGCTGAGACTATATATGACGAGAAATGCTTGGTA
TATTCGCCCGATCAACTCGGAAGCTGTCCCGTCTATGAAGAACATTTGGA
AACGATGAAATTTATCACTCAAACTGGAAAAGTATTAGAGAAAACGGTTT
ACCAGAATTTCAACGTGGACACTTTGCCGGATAAATTACGAATCAGCTGG
TTTCAGTTATATGGACAGTGCCTTAAAAATGGGGGACTAGCCTTGCCAGC
GTCTGATCCTTATCGTGAGAACATCTTAGCAGCTGGGAAGGCTGAGAAAT
TAATCGGCCAGATGGAGAATGAATATTCAACTATTATTAATTGTTTAATT
TTAAAAAACATTCAAGAATTAAGATTGTATCACCAATATATAGTTGTCAC
TTTATATGATATTTTCCAAAAGTATAGAATGGCTCTCAGATTTGACGAAG
AAAAGGCCACAAGATTCGAAAGTAGTTCGGATTACTTGAAAAATCGCAGA
TCAGAATTTAAAAATATTCACAGTGATTTACATGATAAAGTTATCAATGT
CTTTGAGGCCCTTAATAGATTAAAGGACTACCTTCCAGCTATAATAGAAG
CCCAGATGAGAGCAAATACTTATACCAATAATTATTTAGAATATATTTTA
GAAAACTTTCGAGACAATAAAAAGATCCAGAATTACATCATTCCTTTGGC
ATCTGTTGGTTCTGAATTAACCTACGATGTTCCCGATTACCGAAGAGCTT
TATATGGAAATGATCTACAAAGAAATAGAAAAAATGTTGAAATAAATGAT
AAAGGCCTAGATTCTCAAAGACCTTATTATGGTAGCCCAAACGATGTTGA
TCCAAACCAAGGAAATCAACAAAGGAAAAATTGCAAAACCCCACAAAACT
CAGGGAAATCTGATGTAAGCAATGGTGGTCGACAGTATCGAAAAAACCTT
AATGTAAACCCAAATGATGCTGGTCCATACAATTATCAAGGCAATGTAAA
GATGAACAACCCTATTAACCCTCAAGATTCAATAAATTCTGAAATAAATG
ATACTAGTAGACAATACCAAAGAACTTATAAAGATGCCCAAGATGATGTT
GATCCATACAATTCCCAGGGCAATAGAAAAATGAAAAGCTCCGTTAACCC
TCAAAATTCAACAATTTCTGATTTAAACGGAAGTCGCCAAAAACGAATAC
AGGATTCTGTTCTCGATGACATAGTCAGAAGGATAGAAAATAGACAAGCT
TATGGAAAAGAAATTACTGGAATACCTTTTAATCGACGTGCTAAATCTAA
TAGTGAAGCTGTAGGGCTACCCAATCAAAGAGATTCTCAATCTACTGGAT
TACCTCCTCAAAGACGTGCAAGATCTAGTAATGAGGCTACAGGGTTACCT
GATGAACGAGATTTTCAATATAATCCTGAATCTCGCCAAAAACCTGTGAA
AACTGGTTGTGGAGGTTCAGGAGCTTGTCTAAAACAGAATAATTCTTCTC
GTGACTCTTCAAAAAGAGGAAGCTCTGTGAAAACGTCTTGTACATCATCA
TCAGCAGGCCGTGTGCATCAACATGGTCATAAACACAATAATCAAGGTAA
AAGCAGTTCTGTTAGAGGAGATATTTCTAAAAATCAAAATTCCTCAAAAT
CAGATAAAAAAGTTGAGATTGACAGCCATACAAAATATCTGCATTCGGCG
TCCGACAGAAACGATCAAGTAGCAACTGACAACATTAAATTGGACAGTTT
GTCAACGTCCAGCTTGAAGCCTGAAGAATTAAATCGCCGAAGTCCAGCTC
ATTATTAATTGTAGTCAAATGAAAATTAATAGTAAACTGTTCCGTC
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protein sequence of SMED30004699-orf-1

>SMED30004699-orf-1 ID=SMED30004699-orf-1|Name=SMED30004699-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=600bp
MGNAETIYDEKCLVYSPDQLGSCPVYEEHLETMKFITQTGKVLEKTVYQN
FNVDTLPDKLRISWFQLYGQCLKNGGLALPASDPYRENILAAGKAEKLIG
QMENEYSTIINCLILKNIQELRLYHQYIVVTLYDIFQKYRMALRFDEEKA
TRFESSSDYLKNRRSEFKNIHSDLHDKVINVFEALNRLKDYLPAIIEAQM
RANTYTNNYLEYILENFRDNKKIQNYIIPLASVGSELTYDVPDYRRALYG
NDLQRNRKNVEINDKGLDSQRPYYGSPNDVDPNQGNQQRKNCKTPQNSGK
SDVSNGGRQYRKNLNVNPNDAGPYNYQGNVKMNNPINPQDSINSEINDTS
RQYQRTYKDAQDDVDPYNSQGNRKMKSSVNPQNSTISDLNGSRQKRIQDS
VLDDIVRRIENRQAYGKEITGIPFNRRAKSNSEAVGLPNQRDSQSTGLPP
QRRARSSNEATGLPDERDFQYNPESRQKPVKTGCGGSGACLKQNNSSRDS
SKRGSSVKTSCTSSSAGRVHQHGHKHNNQGKSSSVRGDISKNQNSSKSDK
KVEIDSHTKYLHSASDRNDQVATDNIKLDSLSTSSLKPEELNRRSPAHY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002089reproductive organ
PLANA:0003304testis primordium
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 426..599
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 279..320
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 488..518
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 363..395
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 565..590
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..458
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..395