Protein kinase domain-containing protein

Overview
NameProtein kinase domain-containing protein
Smed IDSMED30004675
Length (bp)643
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein kinase domain-containing protein (SMED30004675) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein kinase domain-containing protein (SMED30004675) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004675

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30004675SMESG000021290.1 Contig2317newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30004675SMESG000021290.1 Contig2317uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
epidermisSMED30004675SMESG000021290.1 dd_Smed_v4_14212_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: GSK3A (glycogen synthase kinase 3 alpha [Source:HGNC Symbol;Acc:HGNC:4616])

HSP 1 Score: 61.2326 bits (147), Expect = 1.357e-10
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      LT+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:   86 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 179
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: GSK3A (glycogen synthase kinase 3 alpha [Source:HGNC Symbol;Acc:HGNC:4616])

HSP 1 Score: 61.2326 bits (147), Expect = 1.526e-10
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      LT+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  168 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 261
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: GSK3A (glycogen synthase kinase 3 alpha [Source:HGNC Symbol;Acc:HGNC:4616])

HSP 1 Score: 61.2326 bits (147), Expect = 1.526e-10
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      LT+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  168 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 261
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: GSK3B (glycogen synthase kinase 3 beta [Source:HGNC Symbol;Acc:HGNC:4617])

HSP 1 Score: 59.6918 bits (143), Expect = 6.258e-10
Identity = 50/186 (26.88%), Postives = 84/186 (45.16%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIF---------------RKKIAYGVFN**WKE-----SRIGHKKGVVNEQE---RKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +C   Q  S FGS K SR++ G+   +V+                  K I  G F   ++        +   K V+ ++    R++      +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D D  ++K+
Sbjct:   13 SCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: AC006486.1 (novel protein)

HSP 1 Score: 57.3806 bits (137), Expect = 6.776e-10
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVD 566
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      LT+P      +M  L   L Y+H+ G+ HRDIK  N+LVD
Sbjct:   81 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVD 166
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: gsk-3 (Glycogen synthase kinase-3 [Source:UniProtKB/Swiss-Prot;Acc:Q9U2Q9])

HSP 1 Score: 59.3066 bits (142), Expect = 2.148e-10
Identity = 33/94 (35.11%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            NH  I+ LKY  YS  +  D +   LI+E +PE++Y + +    +   +P      +M  LL  L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   85 NHPNIVKLKYFFYSSGEKKDELYLNLILEYVPETVYRVARHYSKQRQQIPMIYVKLYMYQLLRSLAYIHSIGICHRDIKPQNLLIDPESGVLKL 178
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: C44H4.6 (pep chromosome:WBcel235:X:14596476:14599158:-1 gene:WBGene00008095.1 transcript:C44H4.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C44H4.6)

HSP 1 Score: 50.0618 bits (118), Expect = 5.726e-9
Identity = 31/95 (32.63%), Postives = 51/95 (53.68%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGDVEQCL--IMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  II L Y+   +      E CL  +ME MP+ L  + ++  + +  +P+     +M  LL G+ ++H + I HRDIK  N+LVD    ++KI
Sbjct:   86 DHPNIIRLLYYYVMQQ-----ENCLNFVMEFMPKDLAYVHRQFAHNDKQMPAYSIKLYMFQLLRGIGFLHLHNIVHRDIKPKNLLVDESNGILKI 175

HSP 2 Score: 27.7202 bits (60), Expect = 5.726e-9
Identity = 16/55 (29.09%), Postives = 31/55 (56.36%), Query Frame = 1 
Query:  121 NAKVILEVKNIQEIGSGTFAKVYSS---------SVKRLRTEYSIDDGKSQELAI 258
            + +V ++  N+Q IG+G+F  VY +         ++K+++ +   D  KS+EL I
Sbjct:   30 DREVEIQFTNLQLIGTGSFGAVYKAVLRENDEPIAIKKIKVD---DRFKSRELTI 81
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: cdk-2 (Cyclin-dependent kinase 2 [Source:UniProtKB/Swiss-Prot;Acc:O61847])

HSP 1 Score: 50.8322 bits (120), Expect = 1.794e-7
Identity = 34/91 (37.36%), Postives = 45/91 (49.45%), Query Frame = 3 
Query:  363 YGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
            Y + +  ++ E +   L +LL+ L   N  LP      FM  LLS L+Y H   I HRD+K  NILV  D  +IKI    + RN    S N
Sbjct:   82 YANSKLYMVFEFIDRDLKNLLEMLEPTNSVLPPNYVKSFMWQLLSALSYCHLRRIVHRDLKPQNILVS-DSGVIKIADFGLARNFSFPSRN 171
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: cdk-11.2 (Cyclin-Dependent Kinase family [Source:UniProtKB/TrEMBL;Acc:Q23357])

HSP 1 Score: 46.595 bits (109), Expect = 5.583e-6
Identity = 28/94 (29.79%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  285 RKIHSFL-SGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILV 563
            R+I+  L +G H  I+++K            E  + ME +   + SL+ ++R++N    + ++   M  LLSG+ +MH   I HRD+K+ N+L+
Sbjct:  350 REINMLLKAGAHENIVNVKEILVGSTK---TEVYMAMEYVEHDVKSLIDKMRSRNQRFKTGQQKTLMSQLLSGIEHMHKLWILHRDLKTSNLLI 440
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: dyf-5 (Serine/threonine-protein kinase dyf-5 [Source:UniProtKB/Swiss-Prot;Acc:B3WFY8])

HSP 1 Score: 46.595 bits (109), Expect = 5.688e-6
Identity = 35/112 (31.25%), Postives = 54/112 (48.21%), Query Frame = 3 
Query:  285 RKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIK 620
            R++ S    NH  II L+      ND        + E M E+LY L+   ++++   P       +  +L GL +MH NG  HRD+K  NI+ +  E L+KI    + R I+
Sbjct:   56 REVKSLKKLNHPNIIKLR-EVIRENDI----LYFVFEFMQENLYELM---KDRDRYFPESVIRNIIYQVLQGLAFMHKNGFFHRDMKPENIMCNGTE-LVKIADFGLAREIR 158
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: gskt (gene:FBgn0046332 transcript:FBtr0085784)

HSP 1 Score: 60.4622 bits (145), Expect = 1.474e-10
Identity = 33/93 (35.48%), Postives = 53/93 (56.99%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            H  II+LK+  +S  +  D V   L+ME +PE+LY + ++      TLP      +M  LL  + Y+H+ G  HRDIK  N+L+D +  ++K+
Sbjct:   83 HDNIITLKWFFFSSGEKRDEVYLNLVMEFLPETLYKVERQYARAKQTLPVNFVRLYMYQLLRSMGYLHSLGFCHRDIKPQNMLLDSETGVLKL 175
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: sgg (gene:FBgn0003371 transcript:FBtr0331752)

HSP 1 Score: 56.6102 bits (135), Expect = 3.261e-9
Identity = 32/105 (30.48%), Postives = 55/105 (52.38%), Query Frame = 3 
Query:  279 QERKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + R++       H  I+ L Y  YS  +  D V   L++E +PE++Y + ++      T+P      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  685 KNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKL 789
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: sgg (gene:FBgn0003371 transcript:FBtr0070471)

HSP 1 Score: 56.6102 bits (135), Expect = 3.674e-9
Identity = 32/105 (30.48%), Postives = 55/105 (52.38%), Query Frame = 3 
Query:  279 QERKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + R++       H  I+ L Y  YS  +  D V   L++E +PE++Y + ++      T+P      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  645 KNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKL 749
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: sgg (gene:FBgn0003371 transcript:FBtr0331751)

HSP 1 Score: 56.6102 bits (135), Expect = 3.771e-9
Identity = 32/105 (30.48%), Postives = 55/105 (52.38%), Query Frame = 3 
Query:  279 QERKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + R++       H  I+ L Y  YS  +  D V   L++E +PE++Y + ++      T+P      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  685 KNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKL 789
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: sgg (gene:FBgn0003371 transcript:FBtr0302185)

HSP 1 Score: 54.6842 bits (130), Expect = 1.492e-8
Identity = 30/90 (33.33%), Postives = 50/90 (55.56%), Query Frame = 3 
Query:  324 IISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            I+ L Y  YS  +  D V   L++E +PE++Y + ++      T+P      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:    9 IVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKL 98
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: gsk3ab (glycogen synthase kinase 3 alpha b [Source:ZFIN;Acc:ZDB-GENE-990714-3])

HSP 1 Score: 59.6918 bits (143), Expect = 3.031e-10
Identity = 31/94 (32.98%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       T+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  132 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFNKSKTTIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 225
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: gsk3ba (glycogen synthase kinase 3 beta, genome duplicate a [Source:ZFIN;Acc:ZDB-GENE-990714-4])

HSP 1 Score: 58.9214 bits (141), Expect = 6.548e-10
Identity = 33/94 (35.11%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  D  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D D  ++K+
Sbjct:  105 DHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: gsk3bb (glycogen synthase kinase 3 beta, genome duplicate b [Source:ZFIN;Acc:ZDB-GENE-090312-2])

HSP 1 Score: 55.4546 bits (132), Expect = 1.005e-8
Identity = 32/94 (34.04%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  D  D V   L+M+ +PE++Y + +        LP      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  105 DHCNIVRLRYFFYSSGDKKDEVYLNLVMDYVPENVYRVARHYSKAKQNLPMVYVKLYMYQLFRSLAYIHSYGICHRDIKPQNLLLDPETAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: gsk3aa (glycogen synthase kinase 3 alpha a [Source:ZFIN;Acc:ZDB-GENE-060503-796])

HSP 1 Score: 52.7582 bits (125), Expect = 6.804e-8
Identity = 28/94 (29.79%), Postives = 50/94 (53.19%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  Y   +  D V   L+++ +PE++Y + +        +P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  159 DHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKL 252
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: stk16 (serine/threonine kinase 16 [Source:ZFIN;Acc:ZDB-GENE-050417-102])

HSP 1 Score: 48.521 bits (114), Expect = 1.936e-6
Identity = 33/130 (25.38%), Postives = 63/130 (48.46%), Query Frame = 3 
Query:  255 HKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPE-SLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMNKV 641
            H +    E + ++      NH  ++SL  + +     G  E  L++  + + S++S+L++LR+K   +P  +    +  +  GL  +H+ G  HRD+K  N+L+D ++    +D          GSMNK 
Sbjct:   54 HDREARKEAQTEVEMHRLFNHPNVLSLTAYTFMERS-GKCEAWLLLPYISKGSVWSVLEKLRDKGSFMPESRILHILHGICEGLKAIHDKGYAHRDLKPTNVLLDENDRPFLMD---------LGSMNKA 173
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: cbarp (CACN subunit beta associated regulatory protein [Source:Xenbase;Acc:XB-GENE-6258190])

HSP 1 Score: 62.003 bits (149), Expect = 6.592e-11
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       +LPS     +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:   79 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFAKAKTSLPSIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 172
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: cbarp (CACN subunit beta associated regulatory protein [Source:Xenbase;Acc:XB-GENE-6258190])

HSP 1 Score: 62.003 bits (149), Expect = 7.192e-11
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       +LPS     +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  105 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFAKAKTSLPSIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: cbarp (CACN subunit beta associated regulatory protein [Source:Xenbase;Acc:XB-GENE-6258190])

HSP 1 Score: 62.003 bits (149), Expect = 7.859e-11
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       +LPS     +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:   89 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFAKAKTSLPSIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 182
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: cbarp (CACN subunit beta associated regulatory protein [Source:Xenbase;Acc:XB-GENE-6258190])

HSP 1 Score: 62.003 bits (149), Expect = 9.120e-11
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       +LPS     +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  120 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFAKAKTSLPSIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 213
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: GSK3B (glycogen synthase kinase 3 beta [Source:NCBI gene;Acc:100489648])

HSP 1 Score: 55.4546 bits (132), Expect = 1.548e-8
Identity = 48/186 (25.81%), Postives = 83/186 (44.62%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIF---------------RKKIAYGVFN**WKE-----SRIGHKKGVVNEQE---RKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +C   Q  S FGS K SR++ G+   +V+                  K I  G F   ++        +   K V+ ++    R++      +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +        LP      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   13 SCKPVQQPSSFGSMKVSRDKDGSKVTTVVATPGQGPDRQQEVSYTDTKVIGNGSFGVVYQAKLCDTGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQALPMIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPETAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gsk3b (glycogen synthase kinase 3 beta [Source:MGI Symbol;Acc:MGI:1861437])

HSP 1 Score: 59.3066 bits (142), Expect = 5.371e-10
Identity = 50/186 (26.88%), Postives = 84/186 (45.16%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIF---------------RKKIAYGVFN**WKE-----SRIGHKKGVVNEQE---RKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +C   Q  S FGS K SR++ G+   +V+                  K I  G F   ++        +   K V+ ++    R++      +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D D  ++K+
Sbjct:   13 SCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gsk3b (glycogen synthase kinase 3 beta [Source:MGI Symbol;Acc:MGI:1861437])

HSP 1 Score: 58.9214 bits (141), Expect = 6.832e-10
Identity = 50/186 (26.88%), Postives = 84/186 (45.16%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIF---------------RKKIAYGVFN**WKE-----SRIGHKKGVVNEQE---RKIHSFLSGNHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +C   Q  S FGS K SR++ G+   +V+                  K I  G F   ++        +   K V+ ++    R++      +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D D  ++K+
Sbjct:   13 SCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gsk3a (glycogen synthase kinase 3 alpha [Source:MGI Symbol;Acc:MGI:2152453])

HSP 1 Score: 57.7658 bits (138), Expect = 2.014e-9
Identity = 31/94 (32.98%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      L  P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  168 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLITPIIYIKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 261
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gsk3a (glycogen synthase kinase 3 alpha [Source:MGI Symbol;Acc:MGI:2152453])

HSP 1 Score: 57.3806 bits (137), Expect = 2.304e-9
Identity = 31/94 (32.98%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      L  P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  168 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLITPIIYIKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 261
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Smok2b (sperm motility kinase 2B [Source:MGI Symbol;Acc:MGI:3037705])

HSP 1 Score: 52.373 bits (124), Expect = 1.065e-7
Identity = 35/81 (43.21%), Postives = 43/81 (53.09%), Query Frame = 3 
Query:  384 LIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPG 626
            LIMEL      SL Q +RN       E R  F   LLS +NY HN GI HRD+K  NI+V+ D  +  ID   +   +KPG
Sbjct:   79 LIMELC--EGKSLYQHIRNAGYLQEDEARALFK-QLLSAMNYCHNQGIVHRDLKPDNIMVEKDGKVKIID-FGLGTQVKPG 155
[Close]
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q60EZ2|GSK2_ORYSJ (Shaggy-related protein kinase GSK2 OS=Oryza sativa subsp. japonica OX=39947 GN=GSK2 PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.563e-10
Identity = 37/118 (31.36%), Postives = 62/118 (52.54%), Query Frame = 3 
Query:  285 RKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHN-NGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
            R++    + +H  +ISLK+  +S     ++   L+ME +PE+LY +L+   N N  +P      +M  L  GL Y+H   G+ HRD+K  N+LVD   + +K+      + + PG  N
Sbjct:  103 RELQLMRAMDHPNVISLKHCFFSTTSRDELFLNLVMEYVPETLYRVLKHYSNANHRMPLIYVKLYMYQLFRGLAYIHTVPGVCHRDVKPQNVLVDPLTHQVKLCDFGSAKTLVPGEPN 220
[Close]
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|A8X5H5|GSK3_CAEBR (Glycogen synthase kinase-3 OS=Caenorhabditis briggsae OX=6238 GN=gsk-3 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.558e-10
Identity = 34/94 (36.17%), Postives = 54/94 (57.45%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            NH  I+ LKY  YS  D  D +   LI+E +PE++Y + +    +  ++P      +M  LL  L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   85 NHPNIVKLKYFFYSSGDKKDELYLNLILEYVPETVYRVARHYSKQRQSIPMIYVKLYMYQLLRSLAYIHSIGICHRDIKPQNLLIDPETGILKL 178
[Close]
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P50873|MRK1_YEAST (Serine/threonine-protein kinase MRK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MRK1 PE=1 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 5.548e-10
Identity = 36/108 (33.33%), Postives = 56/108 (51.85%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHN-NGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
            H   + L+Y+ Y +++  +V   L+++ MP+SLY  L+   N  + +P  +  F+   L   LNY+HN   I HRDIK  N+LVD      KI      + +KP   N
Sbjct:  214 HPNTVGLQYYFYEKDEEDEVYLNLVLDYMPQSLYQRLRHFVNLKMQMPRVEIKFYAYQLFKALNYLHNVPRICHRDIKPQNLLVDPTTFSFKICDFGSAKCLKPDQPN 321
[Close]
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P49840|GSK3A_HUMAN (Glycogen synthase kinase-3 alpha OS=Homo sapiens OX=9606 GN=GSK3A PE=1 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 7.328e-10
Identity = 32/94 (34.04%), Postives = 53/94 (56.38%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D +   L++E +PE++Y + +      LT+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  168 DHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLTIPILYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 261
[Close]
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|P83101|GSK3H_DROME (Putative glycogen synthase kinase-3 homolog OS=Drosophila melanogaster OX=7227 GN=gskt PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.478e-9
Identity = 33/93 (35.48%), Postives = 53/93 (56.99%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            H  II+LK+  +S  +  D V   L+ME +PE+LY + ++      TLP      +M  LL  + Y+H+ G  HRDIK  N+L+D +  ++K+
Sbjct:   83 HDNIITLKWFFFSSGEKRDEVYLNLVMEFLPETLYKVERQYARAKQTLPVNFVRLYMYQLLRSMGYLHSLGFCHRDIKPQNMLLDSETGVLKL 175
[Close]
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A1L0BZI5 (CIC11C00000001315 OS=[Candida] intermedia OX=45354 GN=SAMEA4029009_CIC11G00000005842 PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.697e-13
Identity = 51/176 (28.98%), Postives = 85/176 (48.30%), Query Frame = 3 
Query:  108 CGKFQCKSYFGSQKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
             GK +   Y  SQ      FG + ++ L+   +IA           R+   K   N   R++      +HR I+ LKY+ Y+ ND  ++   LI+E +PE+LY       +K L++PS +   +   +   LNY+H+ GI HRDIK  N+L++ D  ++K+      + + P   N
Sbjct:   16 TGKLESIQYTQSQMVGHGSFGVVFQTQLLPSNEIA--------AIKRVLQDKRFKN---RELQIMKLVHHRNIVDLKYYFYTNNDKNELYLNLILEFVPETLYKASHYYVSKRLSMPSLEVKLYTYQMFRALNYIHSQGICHRDIKPQNLLINADTGVLKLCDFGSAKILNPAEPN 180
[Close]
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: Q6BM28 (DEHA2F08756p OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=DEHA2F08756g PE=3 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 2.032e-12
Identity = 50/176 (28.41%), Postives = 85/176 (48.30%), Query Frame = 3 
Query:  108 CGKFQCKSYFGSQKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
             G+ Q   Y  SQ      FG + ++ ++   +IA           R+   K   N   R++      +HR I+ LKY+ Y+ ND  ++   LI+E +PE+LY       +K L++PS +   +   +L  LNY+H+ GI HRDIK  N+L++ +   +K+      + + P   N
Sbjct:   16 TGELQTIQYTQSQMVGHGSFGVVFETQILPSNEIA--------AIKRVLQDKRFKN---RELQIMKLVHHRNIVDLKYYFYTNNDKSELYLNLILEFVPETLYKASHYYVSKRLSMPSLEVKLYTYQMLRALNYIHSQGICHRDIKPQNLLINPETGELKLCDFGSAKILNPAEPN 180
[Close]
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2H0ZU47 (Uncharacterized protein OS=Candida auris OX=498019 GN=B9J08_003732 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.951e-12
Identity = 46/162 (28.40%), Postives = 82/162 (50.62%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + GK +   Y  SQ      FG + ++ L+   +IA           ++   K   N   R++      +HR ++ L+Y+ Y+ ND  ++   LI+E +PE+LY       +K L++PS +   +   +   LNY+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   15 STGKLESIQYTKSQMVGHGSFGVVFQTQLLPSNEIA--------AIKQVLQDKRFKN---RELQIMKLVHHRNVVDLRYYFYTNNDKNELFLNLILEFVPETLYKASHYYVSKRLSMPSLEVKLYTYQMFRALNYIHSQGICHRDIKPQNLLIDPETGVLKL 165
[Close]
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A510NYL9 (Hypothetical_protein OS=Candida auris OX=498019 GN=CAJCM15448_03500 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.951e-12
Identity = 46/162 (28.40%), Postives = 82/162 (50.62%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + GK +   Y  SQ      FG + ++ L+   +IA           ++   K   N   R++      +HR ++ L+Y+ Y+ ND  ++   LI+E +PE+LY       +K L++PS +   +   +   LNY+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   15 STGKLESIQYTKSQMVGHGSFGVVFQTQLLPSNEIA--------AIKQVLQDKRFKN---RELQIMKLVHHRNVVDLRYYFYTNNDKNELFLNLILEFVPETLYKASHYYVSKRLSMPSLEVKLYTYQMFRALNYIHSQGICHRDIKPQNLLIDPETGVLKL 165
[Close]
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A2V1A8W3 (Protein kinase domain-containing protein OS=[Candida] duobushaemulonis OX=1231522 GN=CXQ87_001992 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.038e-12
Identity = 46/162 (28.40%), Postives = 82/162 (50.62%), Query Frame = 3 
Query:  105 NCGKFQCKSYFGSQKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            + GK +   Y  SQ      FG + ++ L+   +IA           ++   K   N   R++      +HR ++ L+Y+ Y+ ND  ++   LI+E +PE+LY       +K L++PS +   +   +   LNY+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:   15 STGKLESIQYTKSQMVGHGSFGVVFQTQLLPSNEIA--------AIKQVLQDKRFKN---RELQIMKLVHHRNVVDLRYYFYTNNDKNELFLNLILEFVPETLYKASHYYVSKRLSMPSLEVKLYTYQMFRALNYIHSQGICHRDIKPQNLLIDPETGVLKL 165
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: gsk3ab (glycogen synthase kinase 3 alpha [Source:NCBI gene;Acc:103036469])

HSP 1 Score: 58.9214 bits (141), Expect = 4.827e-10
Identity = 31/94 (32.98%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       T+P      +M  L   L Y+H+ G+ HRDIK  N+LVD D  ++K+
Sbjct:  132 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHFNKAKTTIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKL 225
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: gsk3ba (glycogen synthase kinase 3 beta [Source:NCBI gene;Acc:103021870])

HSP 1 Score: 56.9954 bits (136), Expect = 2.042e-9
Identity = 32/94 (34.04%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  D  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  105 DHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPETAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: gsk3ba (glycogen synthase kinase 3 beta [Source:NCBI gene;Acc:103021870])

HSP 1 Score: 56.9954 bits (136), Expect = 2.498e-9
Identity = 32/94 (34.04%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  D  D V   L+++ +PE++Y + +       TLP      +M  L   L Y+H+ GI HRDIK  N+L+D +  ++K+
Sbjct:  105 DHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPETAVLKL 198
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: gsk3aa (glycogen synthase kinase-3 alpha-like [Source:NCBI gene;Acc:103032471])

HSP 1 Score: 55.8398 bits (133), Expect = 6.534e-9
Identity = 29/94 (30.85%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +        +P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  126 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFNKAKTIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKL 219
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: gsk3aa (glycogen synthase kinase-3 alpha-like [Source:NCBI gene;Acc:103032471])

HSP 1 Score: 55.4546 bits (132), Expect = 6.876e-9
Identity = 29/94 (30.85%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +        +P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  126 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFNKAKTIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKL 219
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: mak (male germ cell-associated kinase [Source:ZFIN;Acc:ZDB-GENE-030131-7279])

HSP 1 Score: 46.2098 bits (108), Expect = 2.536e-6
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 3 
Query:  285 RKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCL--IMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIK 620
            R++ S    NH  +I LK      ND      CL  + E M E+LY L+   +++N   P       M  +L GL ++H +G  HRD+K  N+L  +   L+KI    + R I+
Sbjct:   49 REVKSLKKLNHANVIKLK-EVIREND------CLYFVFEYMKENLYQLM---KDRNKLFPESMIRNIMYQILQGLAFIHKHGFFHRDMKPENLLC-MGPELVKIADFGLAREIR 151
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: MAPK6 (mitogen-activated protein kinase 6 [Source:HGNC Symbol;Acc:HGNC:6879])

HSP 1 Score: 45.8246 bits (107), Expect = 2.843e-6
Identity = 24/71 (33.80%), Postives = 38/71 (53.52%), Query Frame = 3 
Query:  411 LYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKP 623
            L + L R+  +   L  E    FM  LL GL Y+H+  + HRD+K  N+ ++ +E L+KI    + R + P
Sbjct:  116 LETDLARVLEQGSALSEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFINTEELLLKIGDFGLARILDP 186
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009383.1 (pep scaffold:Pmarinus_7.0:GL478512:51:5375:-1 gene:ENSPMAG00000008486.1 transcript:ENSPMAT00000009383.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.5134 bits (101), Expect = 5.204e-6
Identity = 26/82 (31.71%), Postives = 47/82 (57.32%), Query Frame = 3 
Query:  384 LIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGS 629
            L+ E + +++  LL+ L N    +P EK   ++  L   +++ H+N I HRDIK  N+L+ +D +++K+      RN+  GS
Sbjct:   55 LVFEYVEKNMLQLLEELPN---GVPPEKVRSYIYQLAKAIHWCHHNNIIHRDIKPENLLIGLD-DVLKLCDFGFARNLSDGS 132
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: cdk4 (cyclin-dependent kinase 4 [Source:ZFIN;Acc:ZDB-GENE-060929-974])

HSP 1 Score: 44.2838 bits (103), Expect = 8.066e-6
Identity = 28/96 (29.17%), Postives = 49/96 (51.04%), Query Frame = 3 
Query:  285 RKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDID 572
            R++ +F    H  ++ L   C +     +    L+ E + + L + L++     L  P++K    M  LLSGL+++H + + HRD+K  NILV  D
Sbjct:   68 RQLETF---EHPNVVRLFDVCATSRTDRETRLTLVFEHVDQDLSTYLEKAPAPGL--PADKIKDMMHQLLSGLDFLHTHRVVHRDLKPQNILVTSD 158
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: MRK1 (Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication [Source:SGD;Acc:S000002237])

HSP 1 Score: 61.6178 bits (148), Expect = 7.989e-12
Identity = 36/108 (33.33%), Postives = 56/108 (51.85%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHN-NGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
            H   + L+Y+ Y +++  +V   L+++ MP+SLY  L+   N  + +P  +  F+   L   LNY+HN   I HRDIK  N+LVD      KI      + +KP   N
Sbjct:  214 HPNTVGLQYYFYEKDEEDEVYLNLVLDYMPQSLYQRLRHFVNLKMQMPRVEIKFYAYQLFKALNYLHNVPRICHRDIKPQNLLVDPTTFSFKICDFGSAKCLKPDQPN 321
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: RIM11 (Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication [Source:SGD;Acc:S000004747])

HSP 1 Score: 60.4622 bits (145), Expect = 2.142e-11
Identity = 36/109 (33.03%), Postives = 58/109 (53.21%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNG-IGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMN 635
            +H  II LKY  Y R+   ++   LI+E MP+SLY  L+   ++   +   +  ++M  L   LNY+H+   + HRDIK  N+LVD +   +K+      + +KP   N
Sbjct:   88 SHINIIDLKYFFYERDSQDEIYLNLILEYMPQSLYQRLRHFVHQRTPMSRLEIKYYMFQLFKSLNYLHHFANVCHRDIKPQNLLVDPETWSLKLCDFGSAKQLKPTEPN 196
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: MCK1 (Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication [Source:SGD;Acc:S000005251])

HSP 1 Score: 56.9954 bits (136), Expect = 2.566e-10
Identity = 42/152 (27.63%), Postives = 69/152 (45.39%), Query Frame = 3 
Query:  144 QKHSRNRFGNICKSVLIFRKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKY---HCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +K  R  FG + ++ L   KK   G F        I         + R++      +H  I+ L+Y   H   +++   V Q L ME +PE+L   + R     L +P +    +   +  G+ Y+H  G+ HRDIK  N+LVD +  ++KI
Sbjct:   39 RKIGRGAFGTVVQAYLTQDKKNWLGPFA-------IKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHLSPQDN--KVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGVCHRDIKPSNVLVDPETGVLKI 181
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: IME2 (Serine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p; human CDK2 can complement ime2 null mutant [Source:SGD;Acc:S000003642])

HSP 1 Score: 46.595 bits (109), Expect = 1.144e-6
Identity = 20/60 (33.33%), Postives = 38/60 (63.33%), Query Frame = 3 
Query:  384 LIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILV 563
            ++ME M ++LY +++  R +  ++PS K    +  +L+GL ++H +   HRD+K  NIL+
Sbjct:  144 IVMECMEQNLYQMMKHRRRRVFSIPSLKS--ILSQILAGLKHIHEHNFFHRDLKPENILI 201
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: PTK2 (Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication [Source:SGD;Acc:S000003820])

HSP 1 Score: 46.595 bits (109), Expect = 1.256e-6
Identity = 26/90 (28.89%), Postives = 47/90 (52.22%), Query Frame = 3 
Query:  294 HSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILV 563
            H+    N  Y++ +    Y+   +G      IMEL  + L+ L++R   KN+  P  +++     +  G+ + H+NGI HRD+K  N+L+
Sbjct:  314 HNVHITNTFYLLKVPTTTYTTRGWG-----FIMELGVKDLFQLMERTGWKNV--PFNEKYCLFKQVAQGIKFCHDNGIAHRDLKPENVLI 396
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO40290 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7S4])

HSP 1 Score: 53.1434 bits (126), Expect = 1.229e-8
Identity = 35/86 (40.70%), Postives = 47/86 (54.65%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDID 572
            H +++ LK   Y R   G +   LIMELM  +LY L+   R K   LP  K   +M  LL  +++ H NGI HRD+K  NIL+  D
Sbjct:   58 HAHVVELKEIIYDRKS-GKL--ALIMELMDMNLYELI---RGKRHYLPEAKVKSYMFQLLKSIDHAHRNGIFHRDVKPENILLKDD 137
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO36371 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIW0])

HSP 1 Score: 51.6026 bits (122), Expect = 5.191e-8
Identity = 28/94 (29.79%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L++  YS  D  + +   L+++ +PE++Y + +       T+P      +M  L   L Y+H  GI HRDIK  N+L+D +  ++K+
Sbjct:  104 DHCNIVRLRWFFYSSGDKKEEIYLNLVLDFIPETVYRVARHYSKAKQTIPMLYIKLYMYQLFRSLTYIHCLGICHRDIKPQNLLLDPETAVLKL 197
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO45693 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RS71])

HSP 1 Score: 49.2914 bits (116), Expect = 3.296e-7
Identity = 28/80 (35.00%), Postives = 45/80 (56.25%), Query Frame = 3 
Query:  384 LIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKP 623
            L+ E+M  +L+++LQ     N +L  +    F+  LL GL Y+H+  + HRDIK  N+LVD +  ++KI      R + P
Sbjct:  101 LVQEVMETNLHTILQ----SNSSLGQDYSKLFLYQLLRGLKYIHSANVLHRDIKPSNLLVDSETLMLKIGDFGQTRVVDP 176
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO30712 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T001])

HSP 1 Score: 48.1358 bits (113), Expect = 9.748e-7
Identity = 19/46 (41.30%), Postives = 30/46 (65.22%), Query Frame = 3 
Query:  444 NLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENL 581
            ++ +P    H +   LL+G+ Y+H  G+ HRDIK  N+L+D D+NL
Sbjct:  106 DIGMPQAMAHKYFKQLLAGVEYLHRQGVTHRDIKPENLLLDSDDNL 151
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO38062 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDX6])

HSP 1 Score: 47.7506 bits (112), Expect = 1.012e-6
Identity = 30/69 (43.48%), Postives = 42/69 (60.87%), Query Frame = 3 
Query:  366 GDVEQCLIMELMPESLYSLLQRLRNK-NLTLPSEKRHFFMCSLLSGLNYMHNN-GIGHRDIKSHNILVD 566
            GDV  C  MELM  SLY L +++ +K +  +P E       S++S L Y+H+N  + HRD+K  NILVD
Sbjct:  128 GDVWIC--MELMKTSLYDLYKQVFSKPDRRIPEEVLGKIAVSVVSALEYLHSNLKVIHRDVKPSNILVD 194
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000016710.2 (glycogen synthase kinase-3 beta [Source:NCBI gene;Acc:101160344])

HSP 1 Score: 58.9214 bits (141), Expect = 5.039e-10
Identity = 30/94 (31.91%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       T+P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  136 DHCNIVRLRYFFYSNGEKKDEVYLNLVLDFVPETVYRVARHFNKAKTTIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAILKL 229
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000027319.1 (glycogen synthase kinase-3 beta [Source:NCBI gene;Acc:101160344])

HSP 1 Score: 58.151 bits (139), Expect = 1.118e-9
Identity = 30/94 (31.91%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       T+P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  132 DHCNIVRLRYFFYSNGEKKDEVYLNLVLDFVPETVYRVARHFNKAKTTIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAILKL 225
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000044063.1 (glycogen synthase kinase-3 beta [Source:NCBI gene;Acc:101160344])

HSP 1 Score: 58.151 bits (139), Expect = 1.137e-9
Identity = 30/94 (31.91%), Postives = 52/94 (55.32%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +       T+P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  172 DHCNIVRLRYFFYSNGEKKDEVYLNLVLDFVPETVYRVARHFNKAKTTIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAILKL 265
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: gsk3aa (glycogen synthase kinase-3 beta [Source:NCBI gene;Acc:101168720])

HSP 1 Score: 55.0694 bits (131), Expect = 1.041e-8
Identity = 29/94 (30.85%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +        +P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:  132 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFNKAKSIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAILKL 225
[Close]
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: gsk3aa (glycogen synthase kinase-3 beta [Source:NCBI gene;Acc:101168720])

HSP 1 Score: 55.0694 bits (131), Expect = 1.103e-8
Identity = 29/94 (30.85%), Postives = 51/94 (54.26%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            +H  I+ L+Y  YS  +  D V   L+++ +PE++Y + +        +P      +M  L   L Y+H+ G+ HRDIK  N+LVD +  ++K+
Sbjct:   98 DHCNIVRLRYFFYSSGEKKDEVYLNLVLDFVPETVYRVARHFNKAKSIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAILKL 191
[Close]
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000021290.1 (SMESG000021290.1)

HSP 1 Score: 243.817 bits (621), Expect = 6.946e-80
Identity = 121/148 (81.76%), Postives = 125/148 (84.46%), Query Frame = 3 
Query:  198 RKKIAYGVFN**WKESRIGHKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKID*LWMRRNIKPGSMNKV 641
            R +  Y + N   K   +  KKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLY LLQRLRNKN+TLPSEKRHFFMCSLLSGLNYMHNN IGHRDIKSHNILVDIDENLIKI        IKPGSMNKV
Sbjct:  103 RLRTEYSIDN--GKSQELAIKKGVVNEQERKIHSFLSGNHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYGLLQRLRNKNVTLPSEKRHFFMCSLLSGLNYMHNNSIGHRDIKSHNILVDIDENLIKIADFGCAEIIKPGSMNKV 248

HSP 2 Score: 167.548 bits (423), Expect = 3.017e-50
Identity = 84/86 (97.67%), Postives = 85/86 (98.84%), Query Frame = 1 
Query:    1 AHSRRSKSSMAQSQTTAHSHRSKYSPSQNPNLYNSIVVNSNAKVILEVKNIQEIGSGTFAKVYSSSVKRLRTEYSIDDGKSQELAI 258
            AHSRRSKSSMAQSQTTAHSHRSKYS SQNPNLYNSIVVNSNAKVILEVKNIQEIGSGTFAKVYSSSVKRLRTEYSID+GKSQELAI
Sbjct:   35 AHSRRSKSSMAQSQTTAHSHRSKYSASQNPNLYNSIVVNSNAKVILEVKNIQEIGSGTFAKVYSSSVKRLRTEYSIDNGKSQELAI 120
[Close]
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000012496.1 (SMESG000012496.1)

HSP 1 Score: 63.5438 bits (153), Expect = 5.757e-12
Identity = 32/93 (34.41%), Postives = 53/93 (56.99%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
             H  +I+L Y+     +  D    LIME +P+++Y L+++ R     +PS+    F   +L GL YMH   I HRDIK  N+LV+++  ++K+
Sbjct:    3 QHPNVIALLYYFIHNGEKDDTYLNLIMEYIPDTVYDLVRKYRQNRTVIPSQYVKIFSYQMLRGLAYMHFKQIVHRDIKPTNLLVNMETGILKL 95
[Close]
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000012496.1 (SMESG000012496.1)

HSP 1 Score: 63.5438 bits (153), Expect = 6.079e-12
Identity = 32/93 (34.41%), Postives = 53/93 (56.99%), Query Frame = 3 
Query:  312 NHRYIISLKYHCYSRNDYGDVEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
             H  +I+L Y+     +  D    LIME +P+++Y L+++ R     +PS+    F   +L GL YMH   I HRDIK  N+LV+++  ++K+
Sbjct:    5 QHPNVIALLYYFIHNGEKDDTYLNLIMEYIPDTVYDLVRKYRQNRTVIPSQYVKIFSYQMLRGLAYMHFKQIVHRDIKPTNLLVNMETGILKL 97
[Close]
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000023764.1 (SMESG000023764.1)

HSP 1 Score: 58.5362 bits (140), Expect = 4.734e-10
Identity = 33/93 (35.48%), Postives = 52/93 (55.91%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            H+ I+ L +  YS  +  D V   L++E +PE++Y + +       T+P      +M  L+  L Y+HN GI HRDIK  N+LVD D  ++K+
Sbjct:  101 HQNIVELLFFFYSNGEKKDEVYLNLVLEFIPETVYRVARHYHKNKQTIPLLFIKLYMYQLMRSLAYIHNLGICHRDIKPQNLLVDPDTGVLKL 193
[Close]
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000023764.1 (SMESG000023764.1)

HSP 1 Score: 58.5362 bits (140), Expect = 5.087e-10
Identity = 33/93 (35.48%), Postives = 52/93 (55.91%), Query Frame = 3 
Query:  315 HRYIISLKYHCYSRNDYGD-VEQCLIMELMPESLYSLLQRLRNKNLTLPSEKRHFFMCSLLSGLNYMHNNGIGHRDIKSHNILVDIDENLIKI 590
            H+ I+ L +  YS  +  D V   L++E +PE++Y + +       T+P      +M  L+  L Y+HN GI HRDIK  N+LVD D  ++K+
Sbjct:  101 HQNIVELLFFFYSNGEKKDEVYLNLVLEFIPETVYRVARHYHKNKQTIPLLFIKLYMYQLMRSLAYIHNLGICHRDIKPQNLLVDPDTGVLKL 193
The following BLAST results are available for this feature:
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GSK3A1.357e-1034.04glycogen synthase kinase 3 alpha [Source:HGNC Symb... [more]
GSK3A1.526e-1034.04glycogen synthase kinase 3 alpha [Source:HGNC Symb... [more]
GSK3A1.526e-1034.04glycogen synthase kinase 3 alpha [Source:HGNC Symb... [more]
GSK3B6.258e-1026.88glycogen synthase kinase 3 beta [Source:HGNC Symbo... [more]
AC006486.16.776e-1034.88novel protein[more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gsk-32.148e-1035.11Glycogen synthase kinase-3 [Source:UniProtKB/Swis... [more]
C44H4.65.726e-932.63pep chromosome:WBcel235:X:14596476:14599158:-1 gen... [more]
cdk-21.794e-737.36Cyclin-dependent kinase 2 [Source:UniProtKB/Swiss... [more]
cdk-11.25.583e-629.79Cyclin-Dependent Kinase family [Source:UniProtKB/... [more]
dyf-55.688e-631.25Serine/threonine-protein kinase dyf-5 [Source:Uni... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gskt1.474e-1035.48gene:FBgn0046332 transcript:FBtr0085784[more]
sgg3.261e-930.48gene:FBgn0003371 transcript:FBtr0331752[more]
sgg3.674e-930.48gene:FBgn0003371 transcript:FBtr0070471[more]
sgg3.771e-930.48gene:FBgn0003371 transcript:FBtr0331751[more]
sgg1.492e-833.33gene:FBgn0003371 transcript:FBtr0302185[more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gsk3ab3.031e-1032.98glycogen synthase kinase 3 alpha b [Source:ZFIN;Ac... [more]
gsk3ba6.548e-1035.11glycogen synthase kinase 3 beta, genome duplicate ... [more]
gsk3bb1.005e-834.04glycogen synthase kinase 3 beta, genome duplicate ... [more]
gsk3aa6.804e-829.79glycogen synthase kinase 3 alpha a [Source:ZFIN;Ac... [more]
stk161.936e-625.38serine/threonine kinase 16 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cbarp6.592e-1134.04CACN subunit beta associated regulatory protein [S... [more]
cbarp7.192e-1134.04CACN subunit beta associated regulatory protein [S... [more]
cbarp7.859e-1134.04CACN subunit beta associated regulatory protein [S... [more]
cbarp9.120e-1134.04CACN subunit beta associated regulatory protein [S... [more]
GSK3B1.548e-825.81glycogen synthase kinase 3 beta [Source:NCBI gene;... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gsk3b5.371e-1026.88glycogen synthase kinase 3 beta [Source:MGI Symbol... [more]
Gsk3b6.832e-1026.88glycogen synthase kinase 3 beta [Source:MGI Symbol... [more]
Gsk3a2.014e-932.98glycogen synthase kinase 3 alpha [Source:MGI Symbo... [more]
Gsk3a2.304e-932.98glycogen synthase kinase 3 alpha [Source:MGI Symbo... [more]
Smok2b1.065e-743.21sperm motility kinase 2B [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q60EZ2|GSK2_ORYSJ1.563e-1031.36Shaggy-related protein kinase GSK2 OS=Oryza sativa... [more]
sp|A8X5H5|GSK3_CAEBR3.558e-1036.17Glycogen synthase kinase-3 OS=Caenorhabditis brigg... [more]
sp|P50873|MRK1_YEAST5.548e-1033.33Serine/threonine-protein kinase MRK1 OS=Saccharomy... [more]
sp|P49840|GSK3A_HUMAN7.328e-1034.04Glycogen synthase kinase-3 alpha OS=Homo sapiens O... [more]
sp|P83101|GSK3H_DROME1.478e-935.48Putative glycogen synthase kinase-3 homolog OS=Dro... [more]
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BLAST of Protein kinase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1L0BZI51.697e-1328.98CIC11C00000001315 OS=[Candida] intermedia OX=45354... [more]
Q6BM282.032e-1228.41DEHA2F08756p OS=Debaryomyces hansenii (strain ATCC... [more]
A0A2H0ZU472.951e-1228.40Uncharacterized protein OS=Candida auris OX=498019... [more]
A0A510NYL92.951e-1228.40Hypothetical_protein OS=Candida auris OX=498019 GN... [more]
A0A2V1A8W33.038e-1228.40Protein kinase domain-containing protein OS=[Candi... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gsk3ab4.827e-1032.98glycogen synthase kinase 3 alpha [Source:NCBI gene... [more]
gsk3ba2.042e-934.04glycogen synthase kinase 3 beta [Source:NCBI gene;... [more]
gsk3ba2.498e-934.04glycogen synthase kinase 3 beta [Source:NCBI gene;... [more]
gsk3aa6.534e-930.85glycogen synthase kinase-3 alpha-like [Source:NCBI... [more]
gsk3aa6.876e-930.85glycogen synthase kinase-3 alpha-like [Source:NCBI... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
mak2.536e-631.58male germ cell-associated kinase [Source:ZFIN;Acc:... [more]
MAPK62.843e-633.80mitogen-activated protein kinase 6 [Source:HGNC Sy... [more]
ENSPMAT00000009383.15.204e-631.71pep scaffold:Pmarinus_7.0:GL478512:51:5375:-1 gene... [more]
cdk48.066e-629.17cyclin-dependent kinase 4 [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
MRK17.989e-1233.33Glycogen synthase kinase 3 (GSK-3) homolog; one of... [more]
RIM112.142e-1133.03Protein kinase; required for signal transduction d... [more]
MCK12.566e-1027.63Dual-specificity ser/thr and tyrosine protein kina... [more]
IME21.144e-633.33Serine/threonine protein kinase involved in activa... [more]
PTK21.256e-628.89Serine/threonine protein kinase; involved in regul... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO402901.229e-840.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO363715.191e-829.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO456933.296e-735.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO307129.748e-741.30Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380621.012e-643.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000016710.25.039e-1031.91glycogen synthase kinase-3 beta [Source:NCBI gene;... [more]
ENSORLT00000027319.11.118e-931.91glycogen synthase kinase-3 beta [Source:NCBI gene;... [more]
ENSORLT00000044063.11.137e-931.91glycogen synthase kinase-3 beta [Source:NCBI gene;... [more]
gsk3aa1.041e-830.85glycogen synthase kinase-3 beta [Source:NCBI gene;... [more]
gsk3aa1.103e-830.85glycogen synthase kinase-3 beta [Source:NCBI gene;... [more]
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BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000021290.16.946e-8081.76SMESG000021290.1[more]
SMESG000012496.15.757e-1234.41SMESG000012496.1[more]
SMESG000012496.16.079e-1234.41SMESG000012496.1[more]
SMESG000023764.14.734e-1035.48SMESG000023764.1[more]
SMESG000023764.15.087e-1035.48SMESG000023764.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004675 ID=SMED30004675|Name=Protein kinase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=643bp
GCACATTCACGTAGATCAAAATCTAGTATGGCGCAAAGTCAAACAACAGC
ACATTCACATAGATCAAAATATAGTCCATCACAAAATCCTAATCTATATA
ATTCAATTGTGGTAAATTCCAATGCAAAAGTTATTTTGGAAGTCAAAAAC
ATTCAAGAAATCGGTTCGGGAACATTTGCAAAAGTGTACTCATCTTCCGT
AAAAAGATTGCGTACGGAGTATTCAATTGATGATGGAAAGAGTCAAGAAT
TGGCCATAAAAAAGGTGTAGTAAACGAACAAGAAAGAAAAATCCATTCAT
TTCTTAGTGGTAATCACAGATATATTATCAGTTTGAAATATCACTGTTAC
TCAAGAAATGATTATGGCGATGTTGAACAATGTCTAATAATGGAATTGAT
GCCAGAATCTTTATATAGCTTGCTTCAAAGACTACGAAATAAAAATCTAA
CCCTACCGAGTGAAAAAAGGCACTTTTTCATGTGCAGTTTGCTTTCTGGT
TTGAATTACATGCACAATAATGGCATTGGACATCGGGACATTAAAAGCCA
TAATATTTTGGTAGACATCGATGAAAATTTGATTAAAATTGACTGACTTT
GGATGCGCAGAAATATAAAGCCAGGTTCAATGAATAAAGTTGA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR017441Protein_kinase_ATP_BS
IPR000719Prot_kinase_dom
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0004672protein kinase activity
GO:0000166nucleotide binding
GO:0004674protein serine/threonine kinase activity
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 54..87