Lipopolysaccharide choline phosphotransferase protein

Overview
NameLipopolysaccharide choline phosphotransferase protein
Smed IDSMED30004616
Length (bp)1416
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Lipopolysaccharide choline phosphotransferase protein (SMED30004616) t-SNE clustered cells

Violin plots show distribution of expression levels for Lipopolysaccharide choline phosphotransferase protein (SMED30004616) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Lipopolysaccharide choline phosphotransferase protein (SMED30004616) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Lipopolysaccharide choline phosphotransferase protein (SMED30004616) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004616

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30004616SMESG000025873.1 dd_Smed_v4_5971_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004616SMESG000043780.1 SMESG000025873.1 Contig45288newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004616SMESG000043780.1 SMESG000025873.1 Contig45288uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A267GME1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030481g1 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 2.727e-23
Identity = 68/187 (36.36%), Postives = 101/187 (54.01%), Query Frame = 1 
Query:  676 LNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCW-----NRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSD-ASACVFK--NHDLKNQC 1212
            L+ +E+  I + +  + SNF Q  LEL L GGS +GSWR+   IP+D DIDF I+  QKP ++  L ++S+  + +F F +TE  NQHW+LGILC        + + CK  +DFFF     G + +  E P+        PL+ RPF   +   P   + ++   Y  D  S C+FK  NH ++  C
Sbjct:  110 LSTDEQSHIYRIVHQLHSNFSQMGLELFLAGGSLLGSWRHLGFIPWDADIDFMIQYNQKPLVRQFLVNLSK--SEQFNFIDTELTNQHWQLGILCGLVGQNLSARKQCKTIVDFFF-GRVKGRVYDRQEAPRKFPIDYVYPLRVRPFGPYLLPAPNQPDQFLTAVYGRDFQSNCLFKYFNH-MRTDC 292
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A267G693 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031085g1 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 2.808e-23
Identity = 68/187 (36.36%), Postives = 101/187 (54.01%), Query Frame = 1 
Query:  676 LNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCW-----NRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSD-ASACVFK--NHDLKNQC 1212
            L+ +E+  I + +  + SNF Q  LEL L GGS +GSWR+   IP+D DIDF I+  QKP ++  L ++S+  + +F F +TE  NQHW+LGILC        + + CK  +DFFF     G + +  E P+        PL+ RPF   +   P   + ++   Y  D  S C+FK  NH ++  C
Sbjct:  110 LSTDEQSHIYRIVHQLHSNFSQMGLELFLAGGSLLGSWRHLGFIPWDADIDFMIQYNQKPLVRQFLVNLSK--SEQFNFIDTELTNQHWQLGILCGLVGQNLSARKQCKTIVDFFF-GRVKGRVYDRQEAPRKFPIDYVYPLRVRPFGPYLLPAPNQPDRFLTAVYGRDFQSNCLFKYFNH-MRTDC 292
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A1I8HJW3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.311e-21
Identity = 68/187 (36.36%), Postives = 101/187 (54.01%), Query Frame = 1 
Query:  676 LNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCW-----NRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSD-ASACVFK--NHDLKNQC 1212
            L+ +E+  I + +  + SNF Q  LEL L GGS +GSWR+   IP+D DIDF I+  QKP ++  L ++S+  + +F F +TE  NQHW+LGILC        + + CK  +DFFF     G + +  E P+        PL+ RPF   +   P   + ++   Y  D  S C+FK  NH ++  C
Sbjct:   40 LSTDEQSHIYRIVHQLHSNFSQMGLELFLAGGSLLGSWRHLGFIPWDADIDFMIQYNQKPLVRQFLVNLSK--SEQFNFIDTELTNQHWQLGILCGLVGQNLSARKQCKTIVDFFFGRVK-GRVYDRQEAPRKFPIDYVYPLRVRPFGPYLLPAPNRPDRFLTAVYGRDFQSNCLFKYFNH-MRTDC 222
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A267E4D8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006412g1 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 5.735e-21
Identity = 71/247 (28.74%), Postives = 121/247 (48.99%), Query Frame = 1 
Query:  652 NSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILC-WNRS--------------METCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDAS-ACVFKNHDLKNQCKMESINCDLLKFKYPYKLRGNFEKFFIEFIVDKIYIKSLFMDFL 1344
            +SCF     +  ++ ++  +L  ++ NF ++ LE+ L  GS +GS R+  +IP+DDDIDF +   QK +++ LL  +S    ++F F + E    HW+L +LC  +RS              +++CKI  D FF    G ++L  +E P  L K D  P++  PF   +   P N +  +  +Y  D   +C F+ H    +C+ + + C+ L   YP          F+  +V +   +S F D L
Sbjct:  147 DSCFN-MSYSMEDKMKVYSALHQLERNFTRRGLEIFLLDGSLLGSRRHFGIIPWDDDIDFMVLREQKFELRQLLIELSH--EDQFRFDDVESVTGHWRLSLLCEADRSKPVQPRYGMSRRFPVKSCKIAADLFFAEIYGDEVLR-VEMPYRLSKRDIYPIQMLPFGPGLMRAPANPQGLLEAEYGRDFEFSCFFEPHSADLRCEEKFLPCEKLSRIYP----------FVRHVVLQKSGRSFFADVL 379
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Match: A0A267DFH5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020560g1 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.100e-19
Identity = 61/164 (37.20%), Postives = 88/164 (53.66%), Query Frame = 1 
Query:  745 NLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCW-----NRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSD-ASACVFK--NHDLKNQC 1212
             LEL L GGS +GSWR+   IP+D DIDF I+  QKP ++  L ++S+  + +F F +TE  NQHW+LGILC        + + CK  +DFFF     G + +  E P+        PL+ RPF   +   P   + ++   Y  D  S C+FK  NH ++  C
Sbjct:    2 GLELFLAGGSLLGSWRHLGFIPWDADIDFMIQYNQKPLVRQFLVNLSK--SEQFNFIDTELTNQHWQLGILCGLVGQNLSARKQCKTIVDFFFG-RVKGRVYDRQEAPRKFPIDYVYPLRVRPFGPYLLPAPNRPDRFLTAVYGRDFQSNCLFKYFNH-MRTDC 161
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO29477 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3I7])

HSP 1 Score: 47.7506 bits (112), Expect = 3.835e-6
Identity = 40/167 (23.95%), Postives = 71/167 (42.51%), Query Frame = 1 
Query:  736 EQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLG----ILCWNRSMETC-------------KIQIDFFFMWDNG-GDLLEFLE-------WPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYS 1161
            ++  +   L  G+ +G+ R+    P+D+DID  I      +++   E + + +     F  TE  +Q++K+     +L   R M +C              + +D   +  N  GD L+F +        P   K +D  P KK  F+   F VP N+E  ++Q Y 
Sbjct:   92 DKHGVRYFLFHGTLLGAVRHQGHNPFDNDIDIAIPEEDFHKLQAAAEELPKGM-----FFQTESTDQYYKIPAYSFLLAKLRDMSSCYTSSMCRGKCHQNGLMVDVSVLPSNSQGDFLDFYKGMKKRFLGPSVHKTTDIYPRKKIKFDGFEFYVPWNYEKMLKQWYG 253
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Match: EDO35854 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SK93])

HSP 1 Score: 46.9802 bits (110), Expect = 7.950e-6
Identity = 40/167 (23.95%), Postives = 71/167 (42.51%), Query Frame = 1 
Query:  736 EQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLG----ILCWNRSMETC-------------KIQIDFFFMWDNG-GDLLEFLE-------WPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYS 1161
            ++  +   L  G+ +G+ R+    P+D+DID  I      +++   E + + +     F  TE  +Q++K+     +L   R M +C              + +D   +  N  GD L+F +        P   K +D  P KK  F+   F VP N+E  ++Q Y 
Sbjct:   92 DKHGVRYFLFHGTLLGAVRHQGHNPFDNDIDIAIPEEDFHKLQAAAEELPKGM-----FFQTESTDQYYKIPAYSFLLAKLRDMSSCYTSLMCRGKCHQNGLMVDVSVLPSNSQGDFLDFYKGMKKRFLGPLVHKTTDIYPRKKIKFDGFEFYVPWNYEKMLKQWYG 253
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000025873.1 (SMESG000025873.1)

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 1 
Query:  418 MRFINQSNDPLSSEIDLKNCILSFFIFYNLQSFLESKNQSMPIYLSFQSSPLRYDILGKECTALFYKGQENLVMKPYDNSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFKNHDLKNQCKMESINCDLLKFKYPYKLRGNFEKFFIEFIVDKIYIKSLFMDFLDSV 1353
            MRFINQSNDPLSSEIDLKNCILSFFIFYNLQSFLESKNQSMPIYLSFQSSPLRYDILGKECTALFYKGQENLVMKPYDNSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFKNHDLKNQCKMESINCDLLKFKYPYKLRGNFEKFFIEFIVDKIYIKSLFMDFLDSV
Sbjct:    1 MRFINQSNDPLSSEIDLKNCILSFFIFYNLQSFLESKNQSMPIYLSFQSSPLRYDILGKECTALFYKGQENLVMKPYDNSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFKNHDLKNQCKMESINCDLLKFKYPYKLRGNFEKFFIEFIVDKIYIKSLFMDFLDSV 312
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000060269.1 (SMESG000060269.1)

HSP 1 Score: 60.077 bits (144), Expect = 9.371e-10
Identity = 54/216 (25.00%), Postives = 100/216 (46.30%), Query Frame = 1 
Query:  634 VMKPYDNSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCWNR-SMETCKIQ-IDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFK--NHDLK---NQCKMESINCDLLKFKYPY 1260
            +  PY++  ++   + P ++  +   L++ KS  ++ N+   L GGS IG+ R+   IP+DDDID  + S  K  + + LE ++Q  N   +     ++   WK   L   + S +  +   +D FF+ +    + +       +KK    PL  R F+N+    P   +  +   Y  D + C     NH  +   +    ++++CDLLK  YP+
Sbjct:   50 IKNPYES--YFHHTMTPIQKSGMLDLLALFKSQMDKFNITFSLYGGSLIGAVRHQGFIPWDDDIDLIVWSSFKSNMISALEELNQTHN---LISYDGKETGKWKFYKLDGKKFSHKPFRYPYLDIFFLKEFNETMFKVEIESYFIKKKHMFPLILRNFDNLQMPTPCATDDILEYMY-GDLNKCTSPSWNHSTEYFVSSSNWKTVSCDLLKPFYPF 259
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000065543.1 (SMESG000065543.1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.166e-9
Identity = 49/217 (22.58%), Postives = 95/217 (43.78%), Query Frame = 1 
Query:  652 NSCFYPFKLNP--NERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNL-------NNRFVFKNTEEQNQHWK---LGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFK--NHDLKNQCKMESINCDLLKFKYPY 1260
            NS   PF++    +ERK + + L +     +  N+  ++ GG+ +GS+R+H +IP+DDD+D  I   Q+ ++K + E  S          +++F  K+ ++    W    L +  +N +       +  +  W           +PK +      PL  RP  N     PK+  +++   Y+   S C     +H ++      +I C  +  K+P+
Sbjct:  120 NSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRGKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQW---------YSYPKEI----VFPLHNRPLLNYSLPSPKDTFAFLVWTYNY-GSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPF 322
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000017956.1 (SMESG000017956.1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.471e-9
Identity = 51/223 (22.87%), Postives = 95/223 (42.60%), Query Frame = 1 
Query:  670 FKLNPNERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFKNTEEQNQHWKLGILCWNRSMETCKIQ-----IDFFFMWDNGGDLLE-------FLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYS-SDASACVFKNHDLKN-QCKMESINCDLLKFKYPYKLRGNFEKFFIE 1296
            +++   ER  + + L++     ++  +   +  G+ +GS+R H  IP+DDDID ++   ++ Q++ +    ++N++N  ++        ++       N S+   K       ID  F   NG  L +       +  +PKS+      PL  RP   +    PK+  +Y    Y  SD     + +H  +N   K   + C +   KYPY  R   +   IE
Sbjct:   19 YQITKQERMTMYRLLAVFDKTMKENGIPFFIHSGTLLGSYRNHDFIPWDDDIDVFVSVAERSQLEKVF--YNKNVSNLILYYTPTHDKIYFS------NDSVSGSKYGWRWPYIDISFYNTNGTHLFDLSPTYGQYDTYPKSI----IFPLTNRPLFTMNLPSPKDAYAYCVWSYGFSDDCVMNYYSHITENLMTKYVKVKCKVFANKYPYVKRYRTDNSVIE 229
[Close]
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Match: SMESG000065540.1 (SMESG000065540.1)

HSP 1 Score: 60.077 bits (144), Expect = 1.515e-9
Identity = 49/217 (22.58%), Postives = 95/217 (43.78%), Query Frame = 1 
Query:  652 NSCFYPFKLNP--NERKEIQKSLSIIKSNFEQQNLELVLTGGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNL-------NNRFVFKNTEEQNQHWK---LGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLKKSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFK--NHDLKNQCKMESINCDLLKFKYPY 1260
            NS   PF++    +ERK + + L +     +  N+  ++ GG+ +GS+R+H +IP+DDD+D  I   Q+ ++K + E  S          +++F  K+ ++    W    L +  +N +       +  +  W           +PK +      PL  RP  N     PK+  +++   Y+   S C     +H ++      +I C  +  K+P+
Sbjct:  114 NSKMKPFQIQMTFDERKMVYRLLELFDQTMKNNNVTYIMYGGTLLGSFRHHDLIPWDDDVDLIIPVKQRVKMKEIFEKFSPEYTFIVVGGHDKFFHKSGKKTRFSWSWPYLDLSYYNENDTHITDIVQTYGQW---------YSYPKEI----VFPLHNRPLLNYSLPSPKDTFAFLVWTYNY-GSPCKMGGYSHMVEGGGPSGNIECKTVSDKFPF 316
The following BLAST results are available for this feature:
BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GME12.727e-2336.36Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G6932.808e-2336.36Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HJW31.311e-2136.36Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267E4D85.735e-2128.74Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DFH51.100e-1937.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO294773.835e-623.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO358547.950e-623.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Lipopolysaccharide choline phosphotransferase protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025873.10.000e+0100.00SMESG000025873.1[more]
SMESG000060269.19.371e-1025.00SMESG000060269.1[more]
SMESG000065543.11.166e-922.58SMESG000065543.1[more]
SMESG000017956.11.471e-922.87SMESG000017956.1[more]
SMESG000065540.11.515e-922.58SMESG000065540.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004616 ID=SMED30004616|Name=Lipopolysaccharide choline phosphotransferase protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1416bp
GACGAAATCAATATTTTACAAACAAAATCAAAATAAATATTTAAATTAAT
GAATTTCAACACCGAGAGATGATAAAAGTTCGAATAAATAGCTGACGAAA
TATCAATAAATCACTTATTTGATTTCCCCAGAATGACAAATTGATGAGGC
ATGCAGCCATATTAATTCCGCCATCTCTGCATTGATTGGTGTATTTTACA
GGATTACACACAAGCATCGGCACACACAACATCGATCGTAAGAACTGGTT
CATTTTGATCAATCATATCTATCTGTATATAATCAAGAGAAAACTGAAGC
AGCCCTTCTTTTTCGATGATCAATAAGAAATACTACATATTAATATTATT
ATTCCTCAGCTTAACAGTCGTGTTTATCGTTTATCAATGTGAAAAATTGA
ACCTCTTATATCTATCAATGAGATTCATCAATCAATCCAATGATCCTTTA
TCAAGCGAGATCGATTTAAAAAATTGCATTCTCTCATTCTTTATTTTCTA
CAATCTTCAAAGTTTTTTGGAAAGTAAAAACCAATCAATGCCTATTTATC
TTTCATTTCAATCTAGTCCATTGCGGTATGATATACTTGGAAAGGAATGC
ACTGCACTTTTCTATAAAGGACAAGAGAATCTCGTTATGAAACCATACGA
CAACTCTTGCTTTTATCCATTTAAACTCAATCCAAATGAACGAAAAGAAA
TTCAAAAATCACTCTCAATAATCAAATCGAACTTTGAACAACAAAATCTG
GAGTTAGTCCTAACCGGTGGCAGCTATATCGGTTCCTGGAGATATCATCA
AATGATTCCATACGACGACGACATCGATTTCTATATTCGTTCATATCAGA
AACCACAAATCAAGACCCTCTTGGAAAGCATGAGCCAAAATCTCAACAAT
CGATTCGTATTCAAAAACACCGAAGAACAAAATCAACATTGGAAATTGGG
AATTCTCTGTTGGAATCGTTCGATGGAAACGTGCAAAATCCAAATCGATT
TCTTTTTCATGTGGGACAATGGCGGAGATCTGTTGGAGTTTTTGGAATGG
CCGAAATCCTTGAAGAAATCAGACTATTTACCATTGAAAAAGCGGCCGTT
TGAGAACATAATTTTCAGTGTGCCAAAAAATTTCGAATCCTACATGAGAC
AACAATACAGCAGTGACGCAAGCGCTTGCGTATTTAAAAATCACGATTTG
AAAAACCAGTGCAAAATGGAATCGATAAATTGTGATCTCCTCAAGTTCAA
ATACCCCTATAAACTGAGGGGAAACTTTGAGAAATTCTTTATCGAATTTA
TCGTGGATAAAATCTATATCAAAAGTTTGTTCATGGATTTTTTAGATTCT
GTATAGCGTTCATAAATAATTCCACTTATGAATTTTTTAAAAATATATTT
TATAATTCGAAAAAAA
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protein sequence of SMED30004616-orf-1

>SMED30004616-orf-1 ID=SMED30004616-orf-1|Name=SMED30004616-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp
MINKKYYILILLFLSLTVVFIVYQCEKLNLLYLSMRFINQSNDPLSSEID
LKNCILSFFIFYNLQSFLESKNQSMPIYLSFQSSPLRYDILGKECTALFY
KGQENLVMKPYDNSCFYPFKLNPNERKEIQKSLSIIKSNFEQQNLELVLT
GGSYIGSWRYHQMIPYDDDIDFYIRSYQKPQIKTLLESMSQNLNNRFVFK
NTEEQNQHWKLGILCWNRSMETCKIQIDFFFMWDNGGDLLEFLEWPKSLK
KSDYLPLKKRPFENIIFSVPKNFESYMRQQYSSDASACVFKNHDLKNQCK
MESINCDLLKFKYPYKLRGNFEKFFIEFIVDKIYIKSLFMDFLDSV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000101muscle cell
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR007074LicD_fam
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007074LicD familyPFAMPF04991LicDcoord: 142..207
e-value: 8.3E-6
score: 26.1
NoneNo IPR availablePANTHERPTHR43404FAMILY NOT NAMEDcoord: 125..312
NoneNo IPR availableTMHMMTMhelixcoord: 7..24