SMED30004582
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004582 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1) HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0 Identity = 390/397 (98.24%), Postives = 391/397 (98.49%), Query Frame = 3 Query: 525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK S R F Sbjct: 74 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 470 HSP 2 Score: 355.91 bits (912), Expect = 3.429e-112 Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310 I PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL Sbjct: 461 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 654 HSP 3 Score: 88.5817 bits (218), Expect = 1.110e-20 Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 3 Query: 147 RDYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272 +DYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF Sbjct: 15 QDYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 56 HSP 4 Score: 31.5722 bits (70), Expect = 1.110e-20 Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = 1 Query: 22 MKKNKLITTKSGKKQ 66 MKKNKLITTKSGKKQ Sbjct: 1 MKKNKLITTKSGKKQ 15
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 390/397 (98.24%), Postives = 391/397 (98.49%), Query Frame = 3 Query: 525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK S R F Sbjct: 54 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 450 HSP 2 Score: 355.525 bits (911), Expect = 2.137e-112 Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310 I PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL Sbjct: 441 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 634 HSP 3 Score: 77.7962 bits (190), Expect = 1.569e-14 Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 3 Query: 165 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF Sbjct: 1 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 36
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1) HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0 Identity = 390/400 (97.50%), Postives = 391/400 (97.75%), Query Frame = 3 Query: 525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKN---SQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKN SQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK S R F Sbjct: 54 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNVLNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 453 HSP 2 Score: 355.525 bits (911), Expect = 2.809e-112 Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310 I PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL Sbjct: 444 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 637 HSP 3 Score: 77.7962 bits (190), Expect = 1.549e-14 Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 3 Query: 165 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF Sbjct: 1 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 36 The following BLAST results are available for this feature:
BLAST of SMED30004582 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004582 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004582 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004582 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004582 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004582 ID=SMED30004582|Name=SMED30004582|organism=Schmidtea mediterranea sexual|type=transcript|length=2356bpback to top protein sequence of SMED30004582-orf-1 >SMED30004582-orf-1 ID=SMED30004582-orf-1|Name=SMED30004582-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp MQLLISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSback to top protein sequence of SMED30004582-orf-2 >SMED30004582-orf-2 ID=SMED30004582-orf-2|Name=SMED30004582-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=404bp MFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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