SMED30004582

Overview
NameSMED30004582
Smed IDSMED30004582
Length (bp)2356
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004582 (SMED30004582) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004582 (SMED30004582) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004582 (SMED30004582) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004582 (SMED30004582) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004582

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004582SMESG000035916.1 SmedASXL_017072SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30004582SMESG000035916.1 dd_Smed_v4_13051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004582SMESG000035916.1 dd_Smed_v4_13051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004582SMESG000035916.1 dd_Smed_v4_13051_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30004582SMESG000035916.1 dd_Smed_v6_13051_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30004582SMESG000035916.1 dd_Smed_v4_13051_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1)

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 390/397 (98.24%), Postives = 391/397 (98.49%), Query Frame = 3 
Query:  525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706
            RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK  S  R F
Sbjct:   74 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 470

HSP 2 Score: 355.91 bits (912), Expect = 3.429e-112
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 
Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310
            I  PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL
Sbjct:  461 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 654

HSP 3 Score: 88.5817 bits (218), Expect = 1.110e-20
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 3 
Query:  147 RDYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272
            +DYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF
Sbjct:   15 QDYSVDMSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 56

HSP 4 Score: 31.5722 bits (70), Expect = 1.110e-20
Identity = 15/15 (100.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   22 MKKNKLITTKSGKKQ 66
            MKKNKLITTKSGKKQ
Sbjct:    1 MKKNKLITTKSGKKQ 15
[Close]
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1)

HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0
Identity = 390/397 (98.24%), Postives = 391/397 (98.49%), Query Frame = 3 
Query:  525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706
            RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK  S  R F
Sbjct:   54 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 450

HSP 2 Score: 355.525 bits (911), Expect = 2.137e-112
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 
Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310
            I  PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL
Sbjct:  441 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 634

HSP 3 Score: 77.7962 bits (190), Expect = 1.569e-14
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 3 
Query:  165 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272
            MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF
Sbjct:    1 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 36
[Close]
BLAST of SMED30004582 vs. Planmine SMEST
Match: SMESG000035916.1 (SMESG000035916.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 390/400 (97.50%), Postives = 391/400 (97.75%), Query Frame = 3 
Query:  525 RIDQMFQEFDLISGSNKRIYEGYGHLVGNXXXXXXXXXXFRKTFHSKN---SQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN-DIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK--SKKRIF 1706
            RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKN   SQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMN +IVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIK  S  R F
Sbjct:   54 RIDQMFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNVLNSQTSTQITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFPLEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRSSSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGTLAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNGNIVRNSTLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLHKEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVNESAQILRKIDEQNIKIENLSETLSMILNKTDDIKTPSTNRCF 453

HSP 2 Score: 355.525 bits (911), Expect = 2.809e-112
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 1 
Query: 1729 ISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVXXXXXXXXXXXXXXTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 2310
            I  PSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL
Sbjct:  444 IKTPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFSVNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNKRHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRINYNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL 637

HSP 3 Score: 77.7962 bits (190), Expect = 1.549e-14
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 3 
Query:  165 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 272
            MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF
Sbjct:    1 MSANRSGQVQTSRFYKYLNDIIIRPTQVLIEEESNF 36
The following BLAST results are available for this feature:
BLAST of SMED30004582 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004582 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000035916.13.429e-11298.24SMESG000035916.1[more]
SMESG000035916.12.137e-11298.24SMESG000035916.1[more]
SMESG000035916.12.809e-11297.50SMESG000035916.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004582 ID=SMED30004582|Name=SMED30004582|organism=Schmidtea mediterranea sexual|type=transcript|length=2356bp
ATTTATTTTAGCAGAAACTTTATGAAGAAAAATAAACTAATAACAACTAA
ATCTGGAAAAAAACAGTATTGAAAATTAATAATTTACAGTTTATTGATGC
CTTAATCTCAATAAAAAAAACTAAATAATACAAAGTTTTTATTTCCAGAG
ATTATTCCGTTGATATGAGCGCCAATCGCTCTGGCCAAGTGCAAACGTCT
CGGTTTTACAAATATCTAAATGATATAATAATTAGACCCACTCAAGTGCT
AATTGAAGAAGAAAGTAATTTCTTGTCTTTAGAAAATGATGAACTAAAAA
AGAATAATGAAGAGTTATCAAATCGGTATGAAATTTGATTAATAAAATTA
TTATAATTTCGGAATTTTTCAATCTGTTTCAATTAATTCTAGATTGTGTA
TAGATTTGAGTACTAAGGGGTTAATTTGTTGGTACATTTGCAGTATTATA
TGGCTAATAAAATAGAATTTGTGGGATGCAAATGAAATAAAATTATAAAA
TATATTTCTATTGTAAATTCTTTTAGAATTGACCAAATGTTCCAAGAATT
CGATTTGATATCAGGATCCAACAAACGAATATATGAAGGTTATGGACATT
TAGTCGGGAATTCTAAAAAATCTAAAAAGGACTCTGATAAATTTCGGAAA
ACATTTCATTCAAAAAACTCACAAACGAGCACTCAAATAACAAATGCAAA
GCTCCAATCGTATGATACAGAGAACGATGGCGCTTTGCATGAAATACAGA
AGAAAGAAAATGAGGCACGCTTGGACAATAGAACTACAGTACTACCAATA
AAAAAAACGGAAAATCGTGATTCTGTCAATTTCCCGTTAGAAATCAAATT
GAAAAGCGTTCAAAATCAAAGTGAAATGCTGCAACGAGACTCTGAAACCG
TTTCCAAATCATTGAAAGGATGTCGTAAGGAAAACTCCATGGCACCAGAA
CAAGTGAAAACGCAATCAAAACAATCCGATAGAAGTTCCTCGCCAATTTA
TCTTGACATATTTGACAATTCGGACCAAGCATTAAAATTTAAATCTGAAC
ATGAAAAATGTAACATTCAAAGAAATAGTAAAAACACAAATTCATTAGTT
GCGAAAATTTACTCAGAAATCCCCGTATTGGGGACCCTGGCAGAATTGCC
GAACGAAACCGGATTGGATACTCAAAGCAAATTTATCAATCCGAGTGCGA
ATTACCCTGAAGATCTGCAATACCTACACCCAGAAGAAGATTTCAATAAG
GAAATTGCAGATATGAATGATATCGTTAGGAATTCTACCTTAATATCATT
GAGCAACAAATCCGACAATTTGACGTCAATTGACACAATTGGACTCTCTG
TCCACAATCTTCTAGATTTGTCAGAAACTCAGAAAGCCATCATGAATTTA
ATTTCACAAAATTCCGAAATTATTCAGGATTTACATAAAGAGCAGTCCAT
GTTATCAAAGTCCAGATCGACTGAGTCCCTTGAAAGTCAAATCTACCGCG
GAACAAAAAACTCCAAGACTTGTCAAAATTATCCCGCAAATGAAAACAGT
TCGATGTGCGTTAATCAATCCGTCCACTGCGTAAATGAGAGTGCCCAAAT
TTTACGTAAAATCGATGAGCAAAACATTAAAATAGAAAATTTGTCCGAAA
CTCTGTCAATGATATTAAACAAAACGGATGACATAAAAAGTAAGAAACGG
ATTTTTATTGCGTTTCATGCAGTTACTAATTTCAGCCCCCTCTACTAACC
GTTGCTTTAACAATGAATCAACTAGTAAATTAGAACAAAATTCTCTACTC
AATAAAGAATTACATCATCATAAAGACTCGGATATTGCCAAATATACGCA
ATCTTTAGAGTTTTCTGTAAATAATCATAACGATAATGATACAATTAATG
ACAATTATAACACTGCATCACTTGCATACTCATTTTCAGACCCCAGTGAC
CCCCCCATTCATTTATTTACCGATAGGTTTTCCTCTGAAAATCTTTTCTC
TGAAAAAACAAACAAAAGACATTCAAATGCCAAAAAACAGACAGAAAATC
TGGAAAATATTAATTCCTTTTTGACTAATGAAGAGACAATTGTCAAGACT
GAAACTTTGTCATTGTCACAGAATCAACCAATGCCAAAATCGTGTAAAAT
ACCGAAAAGAATTAATTATAATAATGGAAAACTTCAATCTAAATCTCATG
ATAAATTTGAAATTTTATTTTCAAATGATTTAGAATCATCATATGAAACC
CTTTATGATCCAATACTTAGTGCAAGATCACTTGTCAATGATGAAAATAT
AGCTTATCTTTAGTAAAATAATATATAAATGTTATATGAATAAACATTTG
GGCTGG
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protein sequence of SMED30004582-orf-1

>SMED30004582-orf-1 ID=SMED30004582-orf-1|Name=SMED30004582-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp
MQLLISAPSTNRCFNNESTSKLEQNSLLNKELHHHKDSDIAKYTQSLEFS
VNNHNDNDTINDNYNTASLAYSFSDPSDPPIHLFTDRFSSENLFSEKTNK
RHSNAKKQTENLENINSFLTNEETIVKTETLSLSQNQPMPKSCKIPKRIN
YNNGKLQSKSHDKFEILFSNDLESSYETLYDPILSARSLVNDENIAYL*
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protein sequence of SMED30004582-orf-2

>SMED30004582-orf-2 ID=SMED30004582-orf-2|Name=SMED30004582-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=404bp
MFQEFDLISGSNKRIYEGYGHLVGNSKKSKKDSDKFRKTFHSKNSQTSTQ
ITNAKLQSYDTENDGALHEIQKKENEARLDNRTTVLPIKKTENRDSVNFP
LEIKLKSVQNQSEMLQRDSETVSKSLKGCRKENSMAPEQVKTQSKQSDRS
SSPIYLDIFDNSDQALKFKSEHEKCNIQRNSKNTNSLVAKIYSEIPVLGT
LAELPNETGLDTQSKFINPSANYPEDLQYLHPEEDFNKEIADMNDIVRNS
TLISLSNKSDNLTSIDTIGLSVHNLLDLSETQKAIMNLISQNSEIIQDLH
KEQSMLSKSRSTESLESQIYRGTKNSKTCQNYPANENSSMCVNQSVHCVN
ESAQILRKIDEQNIKIENLSETLSMILNKTDDIKSKKRIFIAFHAVTNFS
PLY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 352..379
NoneNo IPR availableCOILSCoilCoilcoord: 56..76
NoneNo IPR availableCOILSCoilCoilcoord: 403..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..151