CULT domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004559 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of CULT domain-containing protein vs. Ensembl Human
Match: CRBN (cereblon [Source:HGNC Symbol;Acc:HGNC:30185]) HSP 1 Score: 61.6178 bits (148), Expect = 5.135e-10 Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT-----DQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + E SWF Y+W+V C C++H+GW F + +F+G+ S L+P Sbjct: 345 MAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 423
BLAST of CULT domain-containing protein vs. Ensembl Human
Match: CRBN (cereblon [Source:HGNC Symbol;Acc:HGNC:30185]) HSP 1 Score: 61.6178 bits (148), Expect = 5.238e-10 Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT-----DQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + E SWF Y+W+V C C++H+GW F + +F+G+ S L+P Sbjct: 346 MAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 424
BLAST of CULT domain-containing protein vs. Ensembl Human
Match: CRBN (cereblon [Source:HGNC Symbol;Acc:HGNC:30185]) HSP 1 Score: 61.2326 bits (147), Expect = 7.374e-10 Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + E SWF Y+W+V C C++H+GW F + +F+G+ S L+P Sbjct: 346 MAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 424
BLAST of CULT domain-containing protein vs. Ensembl Celegans
Match: R08B4.3 (pep chromosome:WBcel235:X:11118959:11121005:-1 gene:WBGene00011139.1 transcript:R08B4.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R08B4.3) HSP 1 Score: 53.5286 bits (127), Expect = 1.139e-8 Identity = 31/121 (25.62%), Postives = 50/121 (41.32%), Query Frame = 1 Query: 100 KFLCRLCGESIVANQFILN--KYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK-------SYTDQFYGVIFSKLV 435 + LCR CG SI ++N Q + + +T + NPE F V A+ G +W+ Y W++ LC CS H+GW+F+ S F G++ ++ Sbjct: 20 ELLCRQCGASITRQSELINITGVDQSQLQYEYDFPLAGKTTKVNVLTNPENQKFHVFGAKTAHLHFHGTPQSHATWYPGYKWTICLCKSCSRHMGWYFEPEKSTAVSEKKSFVGLVLDNVI 140
BLAST of CULT domain-containing protein vs. Ensembl Fly
Match: ohgt (gene:FBgn0037780 transcript:FBtr0082174) HSP 1 Score: 59.3066 bits (142), Expect = 1.656e-9 Identity = 38/119 (31.93%), Postives = 56/119 (47.06%), Query Frame = 1 Query: 88 TFDDK--FLCRLCGESIV--ANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIG-QGTP--EFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIF 423 TF D+ F CR C S+ ++ F ++K H + + + NPEG + E T Y+ + IG G P +FSWF Y W +ILC C+ H+GW FK+ +F Sbjct: 446 TFKDETLFFCRYCNSSLALCSDLFAMSK---HGVQTQ--------------YCNPEGYIHETNTVYRVISHAIGYSGEPSTKFSWFPGYQWHIILCKFCAQHVGWEFKAVHPNLTPKVF 547
BLAST of CULT domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-51h9.7 (si:ch211-51h9.7 [Source:ZFIN;Acc:ZDB-GENE-081104-235]) HSP 1 Score: 78.1814 bits (191), Expect = 3.418e-17 Identity = 34/96 (35.42%), Postives = 53/96 (55.21%), Query Frame = 1 Query: 103 FLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK 390 LCR CG + + + S+ A+++RN ++ + + +Q F NP G FEV T+ +A+ FSW+ +SW+ CPRC THLGW F+ Sbjct: 25 LLCRSCGHELAEDTDLSFVPSRMALSHRNDTVIGGKRVSVQLFENPHGFQFEVVTFRRADVLKHWPADRHFSWYPGHSWTAATCPRCKTHLGWAFQ 120
BLAST of CULT domain-containing protein vs. Ensembl Zebrafish
Match: crbn (cereblon [Source:NCBI gene;Acc:445491]) HSP 1 Score: 59.3066 bits (142), Expect = 1.776e-9 Identity = 29/74 (39.19%), Postives = 42/74 (56.76%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 NP G V E T YKA N +IG+ + SWF Y+W++ C CS+H+GW F + +F+G+ S L+P Sbjct: 356 NPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSALLP 429
BLAST of CULT domain-containing protein vs. Ensembl Xenopus
Match: mgc69309 (mgc69309 protein [Source:Xenbase;Acc:XB-GENE-5903348]) HSP 1 Score: 56.9954 bits (136), Expect = 1.263e-8 Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 NP G V E T YKA N +++G+ + E SWF ++W++ C C +H+GW F + +F+G+ S L P Sbjct: 320 NPHGYVHETLTVYKAFNLSLVGRPSTENSWFPGFAWTIAQCRVCGSHMGWKFTAVRKDLSPQKFWGLTRSALQP 393
BLAST of CULT domain-containing protein vs. Ensembl Xenopus
Match: mgc69309 (mgc69309 protein [Source:Xenbase;Acc:XB-GENE-5903348]) HSP 1 Score: 56.9954 bits (136), Expect = 1.287e-8 Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 + + NP G V E T YKA N +++G+ + E SWF ++W++ C C +H+GW F + +F+G+ S L P Sbjct: 349 MAAYVNPHGYVHETLTVYKAFNLSLVGRPSTENSWFPGFAWTIAQCRVCGSHMGWKFTAVRKDLSPQKFWGLTRSALQP 427
BLAST of CULT domain-containing protein vs. Ensembl Xenopus
Match: mgc69309 (mgc69309 protein [Source:Xenbase;Acc:XB-GENE-5903348]) HSP 1 Score: 56.9954 bits (136), Expect = 1.433e-8 Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 + + NP G V E T YKA N +++G+ + E SWF ++W++ C C +H+GW F + +F+G+ S L P Sbjct: 329 MAAYVNPHGYVHETLTVYKAFNLSLVGRPSTENSWFPGFAWTIAQCRVCGSHMGWKFTAVRKDLSPQKFWGLTRSALQP 407
BLAST of CULT domain-containing protein vs. Ensembl Xenopus
Match: mgc69309 (mgc69309 protein [Source:Xenbase;Acc:XB-GENE-5903348]) HSP 1 Score: 56.9954 bits (136), Expect = 1.543e-8 Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 NP G V E T YKA N +++G+ + E SWF ++W++ C C +H+GW F + +F+G+ S L P Sbjct: 342 NPHGYVHETLTVYKAFNLSLVGRPSTENSWFPGFAWTIAQCRVCGSHMGWKFTAVRKDLSPQKFWGLTRSALQP 415
BLAST of CULT domain-containing protein vs. Ensembl Mouse
Match: Crbn (cereblon [Source:MGI Symbol;Acc:MGI:1913277]) HSP 1 Score: 57.7658 bits (138), Expect = 6.065e-9 Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT-----DQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + SWF Y+W++ C C++H+GW F + +F+G+ S L+P Sbjct: 349 MAAYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 427
BLAST of CULT domain-containing protein vs. Ensembl Mouse
Match: Crbn (cereblon [Source:MGI Symbol;Acc:MGI:1913277]) HSP 1 Score: 57.7658 bits (138), Expect = 6.111e-9 Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT-----DQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + SWF Y+W++ C C++H+GW F + +F+G+ S L+P Sbjct: 348 MAAYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 426
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Match: sp|Q96SW2|CRBN_HUMAN (Protein cereblon OS=Homo sapiens OX=9606 GN=CRBN PE=1 SV=1) HSP 1 Score: 61.6178 bits (148), Expect = 2.516e-9 Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT-----DQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + E SWF Y+W+V C C++H+GW F + +F+G+ S L+P Sbjct: 346 MAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 424
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5R6Y2|CRBN_PONAB (Protein cereblon OS=Pongo abelii OX=9601 GN=CRBN PE=2 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 2.872e-9 Identity = 30/79 (37.97%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSY-----TDQFYGVIFSKLVP 438 + + NP G V E T YKA N +IG+ + E SWF Y+W+V C C++H+GW F + +F+G+ S L+P Sbjct: 333 MAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLP 411
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Match: sp|B4PVI7|CRBN_DROYA (Protein cereblon homolog OS=Drosophila yakuba OX=7245 GN=GE26004 PE=3 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 9.571e-9 Identity = 38/119 (31.93%), Postives = 56/119 (47.06%), Query Frame = 1 Query: 88 TFDDK--FLCRLCGESIV--ANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIG-QGTP--EFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIF 423 TF D+ F CR C S+ A+ F ++K H + + + NP+G + E T Y+ + IG G P +FSWF Y W +ILC C+ H+GW FK+ +F Sbjct: 449 TFKDESLFFCRYCNSSLAHCADLFAMSK---HGVQTQ--------------YCNPDGYIHETNTVYRVMSHAIGYSGEPSTKFSWFPGYQWHIILCKFCAQHVGWEFKAVQPNLTPRVF 550
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Match: sp|B3P4M4|CRBN_DROER (Protein cereblon homolog OS=Drosophila erecta OX=7220 GN=GG17313 PE=3 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 1.254e-8 Identity = 38/119 (31.93%), Postives = 56/119 (47.06%), Query Frame = 1 Query: 88 TFDDK--FLCRLCGESIV--ANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIG-QGTP--EFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIF 423 TF D+ F CR C S+ A+ F ++K H + + + NP+G + E T Y+ + IG G P +FSWF Y W +ILC C+ H+GW FK+ +F Sbjct: 447 TFTDESLFFCRYCNSSLAHCADLFAMSK---HGVQTQ--------------YCNPDGYIHETNTVYRVMSHAIGYSGEPSTKFSWFPGYQWHIILCKFCAQHVGWEFKAVQPNLTPRVF 548
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Match: sp|Q68EH9|CRBN_DANRE (Protein cereblon OS=Danio rerio OX=7955 GN=crbn PE=1 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 1.420e-8 Identity = 29/74 (39.19%), Postives = 42/74 (56.76%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 NP G V E T YKA N +IG+ + SWF Y+W++ C CS+H+GW F + +F+G+ S L+P Sbjct: 341 NPHGYVHETLTVYKASNLNLIGRPSTLHSWFPGYAWTIAQCRTCSSHMGWKFSAVKKDLSPPRFWGLTRSALLP 414
BLAST of CULT domain-containing protein vs. TrEMBL
Match: A0A0L8FMN4 (CULT domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22013893mg PE=4 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 1.777e-20 Identity = 44/119 (36.97%), Postives = 65/119 (54.62%), Query Frame = 1 Query: 103 FLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTDQ--------FYGVIFSKLV 435 +CR CG + +++ S A+ RN +I S ++Q F+NPEG FEV T+ KA + E +WF +SWS+++CP C H+GWFF+S+ FYG+I S LV Sbjct: 48 LICRQCGHEVACIDDLVHIPSSLALRQRNDTIQSKDKTIVQLFQNPEGKYFEVITFSKAEVLKADKPHKEDTWFPGFSWSIVICPTCGVHMGWFFESFDSDYHTGEKKSFYGLILSHLV 166
BLAST of CULT domain-containing protein vs. TrEMBL
Match: A0A1S3HXW9 (uncharacterized protein LOC106159203 OS=Lingula unguis OX=7574 GN=LOC106159203 PE=4 SV=1) HSP 1 Score: 94.3597 bits (233), Expect = 1.740e-19 Identity = 45/127 (35.43%), Postives = 67/127 (52.76%), Query Frame = 1 Query: 91 FDDKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYT------------DQFYGVIFSKLV 435 F+D LCR CG + +L+ S A+ RN +I + +L+Q F+NP+G+ FE+ KA+ + + E SWF +W V +CPRC HLGWFF+ +T D F G+I L+ Sbjct: 61 FEDYLLCRHCGHEVAKASHLLHVPSPLALRQRNDTILGVKEVLIQLFQNPQGSQFEIIAAQKADVYKLNKAFIEHSWFPGLTWRVTVCPRCGHHLGWFFEPHTHDLPETYPEAIKDGFAGLILENLL 187
BLAST of CULT domain-containing protein vs. TrEMBL
Match: A0A0B6Z9X6 (CULT domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF54211 PE=4 SV=1) HSP 1 Score: 89.3521 bits (220), Expect = 9.205e-18 Identity = 43/121 (35.54%), Postives = 64/121 (52.89%), Query Frame = 1 Query: 103 FLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD----------QFYGVIFSKLV 435 LCR CG + Q +++ S+ A+ RN ++N+N +L+Q F NP+G FEV T +A + SWF Y+WS+ CPRC THLGW F + + F G+I K++ Sbjct: 43 LLCRRCGYDVAKVQDLISIPSKSALRQRNDTVNNNNRILIQLFANPQGMYFEVITSSEAEVERSAERYITDSWFPGYTWSIAKCPRCGTHLGWAFNAVAEGAETGVMKVLSFVGLILDKVM 163
BLAST of CULT domain-containing protein vs. TrEMBL
Match: A0A210QMV2 (Protein cereblon OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23493 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 1.870e-17 Identity = 46/138 (33.33%), Postives = 68/138 (49.28%), Query Frame = 1 Query: 97 DKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK---------SYTDQFYGVIFSKLVPMQ----LNHYFQVYS 471 D LCR CG +V ++N S+ A+ RN + +L+Q F+NP FEV T KA+ Q + +WF NYSW + CP C H+GW ++ +QFYG++ L+P Q L H+ + Y Sbjct: 41 DYLLCRKCGHEVVRAANLINIPSKVALRQRNSTFGGVPEILIQQFKNPHDNSFEVITTRKADVLEDLQKYSDDTWFPNYSWWITKCPHCGQHMGWTYRINSQGTSTDGRPEQFYGLVLDNLLPQQEAETLIHFPKAYG 178
BLAST of CULT domain-containing protein vs. TrEMBL
Match: A0A2U9BTY1 (CULT domain-containing protein OS=Scophthalmus maximus OX=52904 GN=SMAX5B_019293 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 2.527e-17 Identity = 38/99 (38.38%), Postives = 57/99 (57.58%), Query Frame = 1 Query: 94 DDKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK 390 D LCR CG + I + S+ A++ RN ++ + + +Q F+NP G FEV T+ +A+ + FSWF +SW+V CPRC+THLGW F+ Sbjct: 49 DAVLLCRACGHELAVGADIRSVPSRRALSGRNDTLVGGRRVDVQLFQNPHGHRFEVITFRQADVRLHWPADQHFSWFPGFSWTVATCPRCATHLGWAFQ 147
BLAST of CULT domain-containing protein vs. Ensembl Cavefish
Match: si:ch211-51h9.7 (si:ch211-51h9.7 [Source:ZFIN;Acc:ZDB-GENE-081104-235]) HSP 1 Score: 72.7886 bits (177), Expect = 1.730e-15 Identity = 34/96 (35.42%), Postives = 53/96 (55.21%), Query Frame = 1 Query: 103 FLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK 390 LCR CG + + + S+ A+++RN ++ + + +Q F NP+G +EV T KA+ F+WF +SW+V CPRC THL W F+ Sbjct: 3 LLCRSCGHEVALERDVSFVPSRLALSHRNNTVLGERRITVQLFENPQGFQYEVLTLKKADVLKHWPADKHFTWFPGFSWTVATCPRCKTHL-WAFQ 97
BLAST of CULT domain-containing protein vs. Ensembl Cavefish
Match: crbn (cereblon [Source:NCBI gene;Acc:103026236]) HSP 1 Score: 57.3806 bits (137), Expect = 6.425e-9 Identity = 27/74 (36.49%), Postives = 41/74 (55.41%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 NP G V E T YKA N ++G+ + SWF Y+W++ C C +H+GW F + +F+G+ S L+P Sbjct: 355 NPHGYVHETLTVYKASNLNLVGRPSTLHSWFPGYAWTIAQCRTCGSHMGWKFSAVKKDLSPPRFWGLTRSALLP 428
BLAST of CULT domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007868.1 (pep scaffold:Pmarinus_7.0:GL481342:3051:16901:1 gene:ENSPMAG00000007114.1 transcript:ENSPMAT00000007868.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 1.443e-10 Identity = 27/79 (34.18%), Postives = 45/79 (56.96%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKANA-TIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 + + NP G V E T +KA++ +IG+ + E+SWF Y+W++ C C +H+GW F + +F+G+ S L P Sbjct: 65 MAAYVNPHGYVHETLTVHKASSLNLIGRPSTEYSWFPGYAWTIAQCRDCGSHMGWKFTATRKDMLPAKFWGLTRSALSP 143
BLAST of CULT domain-containing protein vs. Ensembl Nematostella
Match: EDO42848 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0B6]) HSP 1 Score: 85.8853 bits (211), Expect = 2.531e-20 Identity = 41/99 (41.41%), Postives = 51/99 (51.52%), Query Frame = 1 Query: 97 DKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKS 393 D LCR CG I + N S+ A+ RN +I L+Q F+NP G FEV T AN G E SWF Y+W + +CPRC HLGW F+ Sbjct: 34 DALLCRRCGAEITFAGHLKNVGSKMALRQRNDTILGVNECLIQLFKNPNGNYFEVITADAANIKQQGGAFTEHSWFPGYAWRIAVCPRCGAHLGWSFED 132
BLAST of CULT domain-containing protein vs. Ensembl Nematostella
Match: EDO28630 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6W7]) HSP 1 Score: 58.151 bits (139), Expect = 1.165e-9 Identity = 31/79 (39.24%), Postives = 42/79 (53.16%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 L + NP G V E T+YKA + G T E SWF Y+W++ C +CS HLGW F + +F+G+ S L P Sbjct: 314 LGAYVNPGGFVHETLTFYKALGIRLRGHPTAENSWFPGYAWTIAECQQCSNHLGWRFSAIKKGLSPGKFWGLTRSSLKP 392
BLAST of CULT domain-containing protein vs. Ensembl Nematostella
Match: EDO40533 (pep supercontig:ASM20922v1:NEMVEscaffold_84:211683:220625:-1 gene:NEMVEDRAFT_v1g106861 transcript:EDO40533 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 58.151 bits (139), Expect = 1.197e-9 Identity = 31/79 (39.24%), Postives = 42/79 (53.16%), Query Frame = 1 Query: 220 LQYFRNPEGTVFEVATYYKA-NATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 L + NP G V E T+YKA + G T E SWF Y+W++ C +CS HLGW F + +F+G+ S L P Sbjct: 314 LGAYVNPGGFVHETLTFYKALGIRLRGHPTAENSWFPGYAWTIAECQQCSNHLGWRFSAIKKGLSPGKFWGLTRSSLKP 392
BLAST of CULT domain-containing protein vs. Ensembl Medaka
Match: si:ch211-51h9.7 (protein cereblon [Source:NCBI gene;Acc:110015676]) HSP 1 Score: 69.707 bits (169), Expect = 1.748e-14 Identity = 33/91 (36.26%), Postives = 52/91 (57.14%), Query Frame = 1 Query: 118 CGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFK 390 CG+ + + I S+ A++ RN ++ + + + +Q F NP G FEV T+ KA+ T FSWF +SW+V CPRC +H+ W F+ Sbjct: 2 CGQELASGADIRFVPSRLALSTRNDTLVNGRRVNVQLFENPHGVQFEVVTFRKADVTQHWPADERFSWFPGFSWTVATCPRCRSHV-WAFQ 91
BLAST of CULT domain-containing protein vs. Ensembl Medaka
Match: crbn (cereblon [Source:NCBI gene;Acc:101155184]) HSP 1 Score: 55.8398 bits (133), Expect = 1.673e-8 Identity = 26/74 (35.14%), Postives = 41/74 (55.41%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKAN-ATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 NP G V E T YKAN ++G+ + SWF Y+W++ C C +H+GW F + +F+G+ S ++P Sbjct: 355 NPHGYVHETLTVYKANNLNLVGRPSTLHSWFPGYAWTIAQCRTCGSHMGWKFTATKKDLSPPRFWGLTRSAMLP 428
BLAST of CULT domain-containing protein vs. Ensembl Medaka
Match: crbn (cereblon [Source:NCBI gene;Acc:101155184]) HSP 1 Score: 55.8398 bits (133), Expect = 1.673e-8 Identity = 26/74 (35.14%), Postives = 41/74 (55.41%), Query Frame = 1 Query: 235 NPEGTVFEVATYYKAN-ATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTD-----QFYGVIFSKLVP 438 NP G V E T YKAN ++G+ + SWF Y+W++ C C +H+GW F + +F+G+ S ++P Sbjct: 355 NPHGYVHETLTVYKANNLNLVGRPSTLHSWFPGYAWTIAQCRTCGSHMGWKFTATKKDLSPPRFWGLTRSAMLP 428
BLAST of CULT domain-containing protein vs. Planmine SMEST
Match: SMESG000077108.1 (SMESG000077108.1) HSP 1 Score: 251.906 bits (642), Expect = 7.160e-85 Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 1 Query: 82 YLTFDDKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIFSKLVPM 441 YLTFDDKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIFSKLVP+ Sbjct: 16 YLTFDDKFLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYKANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKSYTDQFYGVIFSKLVPV 135
BLAST of CULT domain-containing protein vs. Planmine SMEST
Match: SMESG000077110.1 (SMESG000077110.1) HSP 1 Score: 234.572 bits (597), Expect = 2.591e-78 Identity = 114/115 (99.13%), Postives = 114/115 (99.13%), Query Frame = -3 Query: 561 DYGSKTPTAIKIPFKNNEVGLFLCEATDRELCTKCRKFPPPDVSKIESKLNYNSILLCDRLRDLLEPISDLELHLLCNDNRLVSQHWPQLENEPQIEEFKEIVQNPITYQKPSFC 905 DYGSKTPTAIKIPFKNNEVGLFLCEATDRELCTKCRKFPPPDVSKIESKLNYNSILLCDRLRDLLEPISDLELHLLCNDNRLVSQHWPQLENEPQIEEFKEIVQ PITYQKPSFC Sbjct: 14 DYGSKTPTAIKIPFKNNEVGLFLCEATDRELCTKCRKFPPPDVSKIESKLNYNSILLCDRLRDLLEPISDLELHLLCNDNRLVSQHWPQLENEPQIEEFKEIVQKPITYQKPSFC 128
BLAST of CULT domain-containing protein vs. Planmine SMEST
Match: SMESG000072072.1 (SMESG000072072.1) HSP 1 Score: 64.3142 bits (155), Expect = 2.977e-11 Identity = 37/118 (31.36%), Postives = 59/118 (50.00%), Query Frame = 1 Query: 103 FLCRLCGESIVANQFILNKYSQHAINNRNYSINSNQTLLLQYFRNPEGTVFEVATYYK-ANATIIGQGTPEFSWFENYSWSVILCPRCSTHLGWFFKS-----YTDQFYGVIFSKLVP 438 FLC C + I + Q I+ ++ + LQ F NP G + E+ T +N +IG+ T E+SWF Y+W+++ C C++H GW F + + FYGV S ++P Sbjct: 473 FLCNNCQQQITSKQKIVCLATEGS---------------LQTFVNPHGHLHEMLTVIGVSNVQMIGRPTVEYSWFPGYAWTIMQCRTCASHTGWKFTACKSGLKPNVFYGVCRSSVIP 575 The following BLAST results are available for this feature:
BLAST of CULT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of CULT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of CULT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of CULT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of CULT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of CULT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of CULT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of CULT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of CULT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of CULT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of CULT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of CULT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of CULT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of CULT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004559 ID=SMED30004559|Name=CULT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=907bpback to top protein sequence of SMED30004559-orf-1 >SMED30004559-orf-1 ID=SMED30004559-orf-1|Name=SMED30004559-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=119bp DYGSKTPTAIKIPFKNNEVGLFLCEATDRELCTKCRKFPPPDVSKIESKLback to top protein sequence of SMED30004559-orf-2 >SMED30004559-orf-2 ID=SMED30004559-orf-2|Name=SMED30004559-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=158bp MLILKLIICFLIIGLYLTFDDKFLCRLCGESIVANQFILNKYSQHAINNRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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