SMED30004553
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004553 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Alignments
SMED30004553 aligns in the following genomic locations:
Homology
BLAST of SMED30004553 vs. Planmine SMEST
Match: SMESG000023121.1 (SMESG000023121.1) HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0 Identity = 331/353 (93.77%), Postives = 334/353 (94.62%), Query Frame = 3 Query: 195 MQDLIEECELEVLKKYHNGRRKSSLIAIPKQKRIRILMIHGHPKIQIASSANRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQESADTCKTKNKNQESADICKTKNKIVIPDDTVNLEYVLEFNMEKEVMYCVDSPKDKLQNSIYRTFQDLVKERTNGGQINVDLTDDRKNKYNIALFNGQLWVATVKYSENEKKKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENHNLTFIESKIKWIVTEVNEEFILDVLNTKHKDVIFSSSNKLNLVVAHKKEFVNNIQNPQHSCQI 1253 MQDLIEECELEVLKKYHNGRRKSSLIAIPKQKRIRILMIHGHPKIQIASSA RVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQES TCKT KNKIVIPDD VNLEYVLEFNMEKEVMYCVDSPKDKLQNSIYRTFQDLVKERTNGGQINVDLTDDRK+KYNIALFNGQLWVATVKYSENEK+KLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIEN NLTFIESKIKWIVTEVNEEFILDVLNTKHKDVIFSSS+KLNLVVAHKKEFVNNIQNPQH CQI Sbjct: 1 MQDLIEECELEVLKKYHNGRRKSSLIAIPKQKRIRILMIHGHPKIQIASSAKRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQESTGTCKT-------------KNKIVIPDDKVNLEYVLEFNMEKEVMYCVDSPKDKLQNSIYRTFQDLVKERTNGGQINVDLTDDRKDKYNIALFNGQLWVATVKYSENEKRKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENCNLTFIESKIKWIVTEVNEEFILDVLNTKHKDVIFSSSSKLNLVVAHKKEFVNNIQNPQHFCQI 340
BLAST of SMED30004553 vs. Planmine SMEST
Match: SMESG000023123.1 (SMESG000023123.1) HSP 1 Score: 87.0409 bits (214), Expect = 2.186e-18 Identity = 102/393 (25.95%), Postives = 161/393 (40.97%), Query Frame = 3 Query: 282 KQKRIRILMIHGHPKIQIASSANRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKS-------------------------KVLIFYESSKFHYDQFR--TALKNLIEKFGRNQE--------------------SAD-----------TCKTKNKNQESADICKTKNKIVIPDDTVNLEY-VLEFNMEKEVMYCVDSPKDKLQ-------NSIYRTFQDLVKERTNGGQINVDLTDDRKNKYNIALFNGQLWVATVKYSENEKKKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENHNLTF-IESKI---KWIVTEVNEEFILDV-----LNTKHKDVIFSSSNKLNLVVAHKKEFVNNIQNP 1235 + K +RIL + P IQI S++ ++++IS AAG+F+ + + KL+LK KVL ES K D +R + + RN E SAD T + DI + + DD +Y V E++ + E ++ +K + NS + +E N G + V+L ++ + + + L +LWV V + N KLH GD +V V+G D+F FQ+ N ++ SI ++RLP AKVF +++ SN I + L F I +K KW +TE+N + IL+V N K + S + V H EF N + P Sbjct: 30 RNKFLRILSLSSVPFIQIVSTSKPTIRDVNISSAAGIFISFKSVNSENVLKLLLKEDKIELRFSDINAFNICKTTFDELFRKVLNKRESIKLFSDNYRHSSGFQTNSCPISRNSEFMNDADNSLLITSKIFLDEDSADNDDQTYVTIVGTVDADLSDNGYIDIGSRDSGVPFCDDIFAYDYEVPEYSSQVEPLFGGYESLEKFEKFRSDNTNSSVFLAMKIREEVLNRGGVKVELDEEFLHSFKVKLIENKLWVCHVNINNN--NKLHVGDRIVTVNGRLPKDVFDFQQICHN-SRASIIIQRLPKAKVFAYDMINSNRQGIKLFKEKLYFKINTKFRREKWKITEINLQNILNVDPAILANIKKQ---LSQERPIMTFVTHSAEFANALSKP 416
BLAST of SMED30004553 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1) HSP 1 Score: 84.3445 bits (207), Expect = 1.201e-17 Identity = 84/361 (23.27%), Postives = 157/361 (43.49%), Query Frame = 3 Query: 282 KQKRIRILMIHGHPKIQIASSANRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQESADTCKTK---NKNQESADICKTKNKIVIPDDTVN------LEYVLEFNMEKEVMYCVD------SPKDKLQNSIY-----------RTFQD--------LVKERTNGGQINVDLTDDRKNKYNIALFNGQLWVATVKYSENEKKKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENHNLTFIESK----IKWIVTEVNEEFILDVLNTKHKDVI------FSSSNKLNLVVAHKKEFVNNIQN 1232 K+KRIRI+ I P IQ+ S +RV K+IDIS AA F+ KLIL K L Y + ++Q + ++ ++ + + ++S + + + NQ + N+ P+ L L F + ++ D P+ + N Y TF D + +E N G + + + + + N +LW++ ++ LH GD +++V+ VND+ +F+E +N+ + + V+RLP A+VF+F+ + + I +E +N + + W +TE+N +N +++I SS + V+ H F+ N+ + Sbjct: 21 KEKRIRIMRIQNLPYIQLILSNSRV-KDIDISSAAKCFIKFNTLNFNRSIKLILARKKLQLYFNDVEVFNQCKRSIDTVLTTYHQERQSGSSSHPRTDSDSNQSDKSDSNSLNENNSPNSASQSLKPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRT---KTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSD 377
BLAST of SMED30004553 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1) HSP 1 Score: 73.1738 bits (178), Expect = 5.988e-14 Identity = 78/354 (22.03%), Postives = 150/354 (42.37%), Query Frame = 3 Query: 303 LMIHGHPKIQIASSANRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQESADTCKTK---NKNQESADICKTKNKIVIPDDTVN------LEYVLEFNMEKEVMYCVD------SPKDKLQNSIY-----------RTFQD--------LVKERTNGGQINVDLTDDRKNKYNIALFNGQLWVATVKYSENEKKKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENHNLTFIESK----IKWIVTEVNEEFILDVLNTKHKDVI------FSSSNKLNLVVAHKKEFVNNIQN 1232 + I P IQ+ S +RV K+IDIS AA F+ KLIL K L Y + ++Q + ++ ++ + + ++S + + + NQ + N+ P+ L L F + ++ D P+ + N Y TF D + +E N G + + + + + N +LW++ ++ LH GD +++V+ VND+ +F+E +N+ + + V+RLP A+VF+F+ + + I +E +N + + W +TE+N +N +++I SS + V+ H F+ N+ + Sbjct: 1 MRIQNLPYIQLILSNSRV-KDIDISSAAKCFIKFNTLNFNRSIKLILARKKLQLYFNDVEVFNQCKRSIDTVLTTYHQERQSGSSSHPRTDSDSNQSDKSDSNSLNENNSPNSASQSLKPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRT---KTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSD 350
BLAST of SMED30004553 vs. Planmine SMEST
Match: SMESG000023122.1 (SMESG000023122.1) HSP 1 Score: 72.4034 bits (176), Expect = 1.118e-13 Identity = 78/357 (21.85%), Postives = 155/357 (43.42%), Query Frame = 3 Query: 303 LMIHGHPKIQIASSANRVFKEIDISEAAGLFLCLEYPGKRSQFKLILKSKVLIFYESSKFHYDQFRTALKNLIEKFGRNQESADTCKTK---NKNQESADICKTKNKIVIPDD-TVNLEYV--------LEFNMEKEVMYCVD------SPKDKLQNSIY-----------RTFQD--------LVKERTNGGQINVDLTDDRKNKYNIALFNGQLWVATVKYSENEKKKLHFGDMVVAVDGISVNDMFSFQEYVRNKTKFSIAVKRLPNAKVFLFNISMSNPPKISIENHNLTFIESK----IKWIVTEVNEEFILDVLNTKHKDVI------FSSSNKLNLVVAHKKEFVNNIQN 1232 + I P IQ+ S +RV K+IDIS AA F+ KLIL K L Y + ++Q + ++ ++ + + ++S + + + NQ + N+ P+ + +L+++ L F + ++ D P+ + N Y TF D + +E N G + + + + + N +LW++ ++ LH GD +++V+ VND+ +F+E +N+ + + V+RLP A+VF+F+ + + I +E +N + + W +TE+N +N +++I SS + V+ H F+ N+ + Sbjct: 1 MRIQNLPYIQLILSNSRV-KDIDISSAAKCFIKFNTLNFNRSIKLILARKKLQLYFNDVEVFNQCKRSIDTVLTTYHQERQSGSSSHPRTDSDSNQSDKSDSNSLNENNSPNSASQSLKFIQPRKLSCQLNFLDGENAIFNKDYEIPEYQPEAQTMNGGYITLETFPFRNKNTFNDSNIFVAMKIREEVCNFGGVTITVGKHLIENIQVKVMNKKLWISDIQNWNEMNASLHVGDRIISVNERPVNDLQTFRECTKNREDYILIVQRLPKARVFVFDKNKLDVKTIKLEKNNFFLTKPTKPKDLDWAITEINRT---KTVNINDQNIISNIQSVISSYIRYLAVIIHPARFIKNLSD 353 The following BLAST results are available for this feature:
BLAST of SMED30004553 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004553 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004553 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004553 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004553 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004553 ID=SMED30004553|Name=SMED30004553|organism=Schmidtea mediterranea sexual|type=transcript|length=1279bpback to top protein sequence of SMED30004553-orf-1 >SMED30004553-orf-1 ID=SMED30004553-orf-1|Name=SMED30004553-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=354bp MQDLIEECELEVLKKYHNGRRKSSLIAIPKQKRIRILMIHGHPKIQIASSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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